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Journal articles on the topic 'Plasma contraction'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Goodyear, L. J., P. A. King, M. F. Hirshman, C. M. Thompson, E. D. Horton, and E. S. Horton. "Contractile activity increases plasma membrane glucose transporters in absence of insulin." American Journal of Physiology-Endocrinology and Metabolism 258, no. 4 (1990): E667—E672. http://dx.doi.org/10.1152/ajpendo.1990.258.4.e667.

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To study the interactions between insulin and contraction on the skeletal muscle glucose transport system, the hindquarters of male rats were perfused in the absence of insulin, in the presence of insulin (30 mU/ml), during contractions induced by sciatic nerve stimulation, or during contractions plus insulin. Compared with control preparations, rates of glucose uptake in the perfused hindquarter were increased by 2.5- and 2.6-fold in the insulin and insulin plus contraction groups, respectively, but not significantly increased in the contraction only preparations. After perfusion, soleus and

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2

Kelley, C., P. D'Amore, H. B. Hechtman, and D. Shepro. "Microvascular pericyte contractility in vitro: comparison with other cells of the vascular wall." Journal of Cell Biology 104, no. 3 (1987): 483–90. http://dx.doi.org/10.1083/jcb.104.3.483.

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Collagen lattices containing bovine retinal pericytes (RPs), vascular smooth muscle cells (VSMCs), pulmonary microvessel endothelial cells (PMECs), or aortic endothelial cells (AECs) were prepared and contraction was quantitated by measuring the resulting change in lattice area. VSMCs were the most efficient at lattice contraction followed by RPs and then PMECs. AECs did not contract the lattices. To document further that these observations represent contraction, cells were grown on inert silicone rubber sheets. Substratum wrinkling was indicative of tension development and quantitated as perc

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3

Yin, Xiaoyan, Nicholas J. D. Gower, Howard A. Baylis, and Kevin Strange. "Inositol 1,4,5-Trisphosphate Signaling Regulates Rhythmic Contractile Activity of Myoepithelial Sheath Cells in Caenorhabditis elegans." Molecular Biology of the Cell 15, no. 8 (2004): 3938–49. http://dx.doi.org/10.1091/mbc.e04-03-0198.

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Intercellular communication between germ cells and neighboring somatic cells is essential for reproduction. Caenorhabditis elegans oocytes are surrounded by and coupled via gap junctions to smooth muscle-like myoepithelial sheath cells. Rhythmic sheath cell contraction drives ovulation and is triggered by a factor secreted from oocytes undergoing meiotic maturation. We demonstrate for the first time that signaling through the epidermal growth factor-like ligand LIN-3 and the LET-23 tyrosine kinase receptor induces ovulatory contractions of sheath cells. Reduction-of-function mutations in the i

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4

Tsymbalyuk, O. V. "Modulating the mechanokinetics of spontaneous contractions of the myometrium of rats using calix[4]arene C-90 – plasma membrane calcium ATPase inhibitor." Studia Biologica 15, no. 2 (2021): 3–14. http://dx.doi.org/10.30970/sbi.1502.652.

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Introduction. Plasma membrane calcium ATPase is a constitutive structure of cells that functions as a high affinity system of releasing Са2+ ions from the cytoplasm and ensures a long-term maintenance of the basal concentration of these cations in the state of dormancy. Currently, there are no satisfactory means for the pharmacological correction of plasma membrane calcium ATPase function. Thus, elaboration, synthesis, and study of substances with the targeted impact on plasma membrane calcium ATPase are topical issues. Previously, we determined the ability of this calix[4]arene in the concent

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5

Brozinick, J. T., G. J. Etgen, B. B. Yaspelkis, and J. L. Ivy. "The effects of muscle contraction and insulin on glucose-transporter translocation in rat skeletal muscle." Biochemical Journal 297, no. 3 (1994): 539–45. http://dx.doi.org/10.1042/bj2970539.

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The effect of electrically induced muscle contraction, insulin (10 m-units/ml) and electrically-induced muscle contraction in the presence of insulin on insulin-regulatable glucose-transporter (GLUT-4) protein distribution was studied in female Sprague-Dawley rats during hindlimb perfusion. Plasma-membrane cytochalasin B binding increased approximately 2-fold, whereas GLUT-4 protein concentration increased approximately 1.5-fold above control with contractions, insulin, or insulin + contraction. Microsomal-membrane cytochalasin B binding and GLUT-4 protein concentration decreased by approx. 30

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6

Budzyn, Klaudia, Philip D. Marley, and Christopher G. Sobey. "Chronic mevastatin modulates receptor-dependent vascular contraction in eNOS-deficient mice." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 287, no. 2 (2004): R342—R348. http://dx.doi.org/10.1152/ajpregu.00156.2004.

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We tested the hypothesis that endothelial nitric oxide (NO) synthase (eNOS)-derived NO modulates rho-kinase-mediated vascular contraction. Because 3-hydroxy-3-methylglutaryl (HMG)-CoA-reductase inhibition can both upregulate eNOS expression and inhibit rhoA/rho-kinase function, a second hypothesis tested was that statin treatment modulates rho-kinase-mediated contraction and that this can occur independently of eNOS. Contractile responses to the receptor-dependent agonists serotonin and phenylephrine but not to the receptor-independent agent KCl were greater in aortic rings from eNOS-null (eNO

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7

Katsuta, Yasumi, Takumi Aramaki, Tatsuya Sekiyama, Katsuaki Satomura, and Kidemasa Okumura. "Plasma volume contraction in portal hypertension." Journal of Hepatology 17 (January 1993): S19—S23. http://dx.doi.org/10.1016/s0168-8278(05)80450-7.

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8

Dumke, C. L., J. Kim, E. B. Arias, and G. D. Cartee. "Role of kallikrein-kininogen system in insulin-stimulated glucose transport after muscle contractions." Journal of Applied Physiology 92, no. 2 (2002): 657–64. http://dx.doi.org/10.1152/japplphysiol.00854.2001.

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Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum protein kininogen (MW > 10,000), on this contraction effect. In vitro electrical stimulation of rat epitrochlearis muscles was performed in 1) rat serum ± kallikrein inhibitors; 2) human plasma (normal or kallikrein-deficient); 3) rat serum ± bradykinin receptor-2 inhibitors; or 4

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9

Gao, J., J. Ren, E. A. Gulve, and J. O. Holloszy. "Additive effect of contractions and insulin on GLUT-4 translocation into the sarcolemma." Journal of Applied Physiology 77, no. 4 (1994): 1597–601. http://dx.doi.org/10.1152/jappl.1994.77.4.1597.

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The maximal effects of insulin and muscle contractions on glucose transport are additive. GLUT-4 is the major glucose transporter isoform expressed in skeletal muscle. Muscle contraction and insulin each induce translocation of GLUT-4 from intracellular sites into the plasma membrane. The purpose of this study was to test the hypothesis that the incremental effect of contractions and insulin on glucose transport is mediated by additivity of the maximal effects of these stimuli on GLUT-4 translocation into the sarcolemma. Anesthetized rats were given insulin by intravenous infusion to raise pla

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10

Perry, Melvin G., Mariela M. Molero, Ararat D. Giulumian, et al. "ETB receptor-deficient rats exhibit reduced contraction to ET-1 despite an increase in ETA receptors." American Journal of Physiology-Heart and Circulatory Physiology 281, no. 6 (2001): H2680—H2686. http://dx.doi.org/10.1152/ajpheart.2001.281.6.h2680.

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Several disease states, including hypertension, are associated with elevations in plasma endothelin-1 (ET-1) and variable changes in vascular contraction to ET-1. The spotting lethal ( sl) rat carries a deletion of the endothelin-B (ETB) receptor gene that prevents expression of functional ETB receptors, resulting in elevated plasma ET-1. On a normal diet, these rats are normotensive and thus provide an opportunity to study the vascular effects of chronically elevated ET-1 in the absence of hypertension. Studies were performed in rats homozygous for the ETBdeficiency ( sl/ sl; n = 8) and in tr

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11

Vaz, M., M. V. Shailesh Kumar, R. N. Kulkarni, D. Rodrigues, and P. S. Shetty. "Variability of Cardiovascular and Plasma Noradrenaline Responses to Sustained Isometric Contraction in Normal Human Subjects." Clinical Science 85, no. 1 (1993): 45–49. http://dx.doi.org/10.1042/cs0850045.

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1. Eight healthy adult males underwent three sustained isometric contractions at 30% of maximal voluntary contraction for 3 min, within a 9 day period. 2. The study focuses on the inter-individual and day-to-day intra-individual variability of cardiovascular and plasma noradrenaline responses to sustained isometric contraction. 3. The results of this study indicate that inter-individual variability is generally greater than intra-individual variability. Diastolic blood pressure was the most sensitive as well as the least variable cardiovascular parameter measured. 4. The variability of plasma

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12

Hart, J. L., C. G. Sobey, and O. L. Woodman. "Cholesterol feeding enhances vasoconstrictor effects of products from rabbit polymorphonuclear leukocytes." American Journal of Physiology-Heart and Circulatory Physiology 269, no. 1 (1995): H1—H6. http://dx.doi.org/10.1152/ajpheart.1995.269.1.h1.

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We have studied the vasoactive properties of products released from rabbit polymorphonuclear leukocytes (PMNs) before and after short-term (4 and 8 wk) dietary supplementation with 1% cholesterol. Plasma cholesterol levels were similar after 4 and 8 wk of cholesterol diet, whereas gross atherosclerotic lesions were present at 4 wk but significantly more extensive after 8 wk. PMN products from all rabbits caused endothelium-dependent contraction of isolated, control (nonatherosclerotic) rabbit aorta submaximally contracted with phenylephrine. However, both 4 and 8 wk of cholesterol feeding resu

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13

Gazis, Diana, Geneviève Gonzalez, and Milton Mendlowitz. "In vivo simultaneous comparison of pressor and uterine responses to a single agonist (oxypressin) in estrous rats." Canadian Journal of Physiology and Pharmacology 65, no. 1 (1987): 6–11. http://dx.doi.org/10.1139/y87-002.

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To try to compare receptor compartment kinetics, receptor binding, and binding–response coupling for two smooth muscle types in vivo, pressor and uterine responses to oxypressin, an equipotent analog of oxytocin and vasopressin, were studied simultaneously in urethane-anesthetized, pentolinium–indomethacin treated rats. Access of peptide to the pressor and uterine receptor compartments and peptide–receptor dissociation rate had a negligible effect on the two responses. During both injections and infusions, the blood pressure response seemed to be determined largely by plasma levels of oxypress

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14

VerPlank, Lynn, and Rong Li. "Cell Cycle-regulated Trafficking of Chs2 Controls Actomyosin Ring Stability during Cytokinesis." Molecular Biology of the Cell 16, no. 5 (2005): 2529–43. http://dx.doi.org/10.1091/mbc.e04-12-1090.

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Cytokinesis requires the coordination of many cellular complexes, particularly those involved in the constriction and reconstruction of the plasma membrane in the cleavage furrow. We have investigated the regulation and function of vesicle transport and fusion during cytokinesis in budding yeast. By using time-lapse confocal microscopy, we show that post-Golgi vesicles, as well as the exocyst, a complex required for the tethering and fusion of these vesicles, localize to the bud neck at a precise time just before spindle disassembly and actomyosin ring contraction. Using mutants affecting cycl

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15

Merry, T. L., R. M. Dywer, E. A. Bradley, S. Rattigan, and G. K. McConell. "Local hindlimb antioxidant infusion does not affect muscle glucose uptake during in situ contractions in rat." Journal of Applied Physiology 108, no. 5 (2010): 1275–83. http://dx.doi.org/10.1152/japplphysiol.01335.2009.

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There is evidence that reactive oxygen species (ROS) contribute to the regulation of skeletal muscle glucose uptake during highly fatiguing ex vivo contraction conditions via AMP-activated protein kinase (AMPK). In this study we investigated the role of ROS in the regulation of glucose uptake and AMPK signaling during low-moderate intensity in situ hindlimb muscle contractions in rats, which is a more physiological protocol and preparation. Male hooded Wistar rats were anesthetized, and then N-acetylcysteine (NAC) was infused into the epigastric artery (125 mg·kg−1·h−1) of one hindlimb (contra

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16

Kawai, Yasuaki, Shigeaki Kobayashi та Toshio Ohhashi. "Existence of two types of postjunctional α-adrenoceptors in the isolated canine internal carotid artery". Canadian Journal of Physiology and Pharmacology 66, № 5 (1988): 655–59. http://dx.doi.org/10.1139/y88-102.

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The pharmacological characteristics of postjunctional α-adrenoceptors in isolated canine internal carotid arteries were investigated by the use of selective agonists and antagonists for α1 and α2-adrenoceptors. Norepinephrine, phenylephrine, and xylazine caused concentration-dependent contractions in the helical strips. The contraction induced by 10−4 M xylazine was significantly smaller than that produced by 10−4 M norepinephrine or 10−4 M phenylephrine. The contraction induced by 10−4 M phenylephrine was almost the same value as that induced by 10−4 M norepinephrine. Phentolamine (10−8 and 1

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17

Tonouchi, Mio, Hideo Hatta, and Arend Bonen. "Muscle contraction increases lactate transport while reducing sarcolemmal MCT4, but not MCT1." American Journal of Physiology-Endocrinology and Metabolism 282, no. 5 (2002): E1062—E1069. http://dx.doi.org/10.1152/ajpendo.00358.2001.

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Rates of lactate uptake into giant sarcolemmal vesicles were determined in vesicles collected from rat muscles at rest and immediately after 10 min of intense muscle contraction. This contraction period reduced muscle glycogen rapidly by 37–82% in all muscles examined ( P < 0.05) except the soleus muscle (no change P > 0.05). At an external lactate concentration of 1 mM lactate, uptake into giant sarcolemmal vesicles was not altered ( P > 0.05), whereas at an external lactate concentration of 20 mM, the rate of lactate uptake was increased by 64% ( P < 0.05). Concomitantly, the pla

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18

Takeshima, Takao, Tokio Shimomura, and Kazuro Takahashi. "Platelet Activation in Muscle Contraction Headache and Migraine." Cephalalgia 7, no. 4 (1987): 239–43. http://dx.doi.org/10.1046/j.1468-2982.1987.0704239.x.

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In migraine, the role of platelets is regarded as an important factor. We investigated plasma beta-thromboglobulin (BTG), platelet factor 4 (PF4), and 5-hydroxytryptamine (5-HT) in migraine patients and muscle contraction headache (MCH) patients during headache-free periods. The mean values of the plasma BTG, PF4, and 5-HT concentrations in the migraine group and the MCH group were significantly higher than those in healthy controls. The mean value of the plasma BTG concentration was significantly higher in the migraine group than in the MCH group, but the differences in the mean plasma PF4 an

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19

Reversi, Alessandra, Eva Loeser, Devaraj Subramanian, Carsten Schultz, and Stefano De Renzis. "Plasma membrane phosphoinositide balance regulates cell shape during Drosophila embryo morphogenesis." Journal of Cell Biology 205, no. 3 (2014): 395–408. http://dx.doi.org/10.1083/jcb.201309079.

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Remodeling of cell shape during morphogenesis is driven by the coordinated expansion and contraction of specific plasma membrane domains. Loss of this coordination results in abnormal cell shape and embryonic lethality. Here, we show that plasma membrane lipid composition plays a key role in coordinating plasma membrane contraction during expansion. We found that an increase in PI(4,5)P2 levels caused premature actomyosin contraction, resulting in the formation of shortened cells. Conversely, acute depletion of PI(4,5)P2 blocked plasma membrane expansion and led to premature actomyosin disasse

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20

Benoit, J. N., D. C. Zawieja, A. H. Goodman, and H. J. Granger. "Characterization of intact mesenteric lymphatic pump and its responsiveness to acute edemagenic stress." American Journal of Physiology-Heart and Circulatory Physiology 257, no. 6 (1989): H2059—H2069. http://dx.doi.org/10.1152/ajpheart.1989.257.6.h2059.

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The contractile properties of the mesenteric collecting lymphatics of the rat were analyzed under control conditions and during periods of enhanced lymph formation using in vivo microscopic techniques. Pressure and diameter were simultaneously monitored in microscopic collecting lymphatics, and lymphatic pump function was analyzed in accordance with basic principles of cardiac mechanics. The lymphatic contractile cycle was divided into two phases of systole and four phases of diastole. Under control conditions, lymphatics contracted with a frequency of 6.4 +/- 0.61 beats/min and ejected approx

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21

Lin, Y. C., C. H. Ho, and F. Grinnell. "Fibroblasts contracting collagen matrices form transient plasma membrane passages through which the cells take up fluorescein isothiocyanate-dextran and Ca2+." Molecular Biology of the Cell 8, no. 1 (1997): 59–71. http://dx.doi.org/10.1091/mbc.8.1.59.

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When fibroblasts contract collagen matrices, the cells activate a Ca(2+)-dependent cyclic AMP signaling pathway. We have found that contraction also stimulates uptake of fluorescein isothiocyanate-dextran molecules from the medium. Our results indicate that fluorescein isothiocyanate-dextran enters directly into the cell cytoplasm through 3- to 5-nm plasma membrane passages. These passages, which reseal in less than 5 s in the presence of divalent cations, also are likely sites of Ca2+ uptake during contraction and the first step in contraction-activated cyclic AMP signaling. The formation of

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22

Xiao, Zuo-Liang, Maria J. Pozo Andrada, Piero Biancani, and Jose Behar. "Reactive oxygen species (H2O2): effects on the gallbladder muscle of guinea pigs." American Journal of Physiology-Gastrointestinal and Liver Physiology 282, no. 2 (2002): G300—G306. http://dx.doi.org/10.1152/ajpgi.00241.2001.

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Reactive oxygen species (ROS) have been implicated in the pathogenesis of muscle dysfunction in acute inflammatory processes. The aim of these studies was to determine the effects of ROS on gallbladder muscle function in vitro. Single muscle cells were obtained by enzymatic digestion. H2O2 (70 μM) caused maximal contraction of up to 14% and blocked the response to CCK-8, ACh, and KCl. It did not affect the contractions induced by guanosine 5′- O-(3-thiotriphosphate), diacylglycerol, and inositol 1,4,5-trisphosphate that circumvent membrane receptors. The contraction induced by H2O2 was inhibit

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23

Finkelstein, D. I., P. Andrianakis, A. R. Luff, and D. W. Walker. "Developmental changes in hindlimb muscles and diaphragm of sheep." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 263, no. 4 (1992): R900—R908. http://dx.doi.org/10.1152/ajpregu.1992.263.4.r900.

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In this study, plasma thyroxine, contractile and histochemical (adenosinetriphosphatase and NADH) characteristics of soleus (SOL), medial gastrocnemius (MG), and extensor digitorum longus (EDL) were examined in 140-day-gestation fetal sheep and in 2-, 5-, and 30-day-old lambs and adult ewes. Electrophoretic separation of myosin heavy chains was also done on all muscles and the diaphragm. There were no differences in the twitch contraction and relaxation times of MG and EDL at the different ages; in contrast SOL contraction times were significantly shorter in the fetus and newborn than in the a

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24

Ferguson, Ken R., Maximilian Bucher, Tais Gorkhover, et al. "Transient lattice contraction in the solid-to-plasma transition." Science Advances 2, no. 1 (2016): e1500837. http://dx.doi.org/10.1126/sciadv.1500837.

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In condensed matter systems, strong optical excitations can induce phonon-driven processes that alter their mechanical properties. We report on a new phenomenon where a massive electronic excitation induces a collective change in the bond character that leads to transient lattice contraction. Single large van der Waals clusters were isochorically heated to a nanoplasma state with an intense 10-fs x-ray (pump) pulse. The structural evolution of the nanoplasma was probed with a second intense x-ray (probe) pulse, showing systematic contraction stemming from electron delocalization during the sol

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25

Evtyukhin, N. V., A. D. Margolin, A. V. Savel'ev, and V. M. Shmelev. "Multiple contraction of gas-discharge plasma in crevice channels." Physics Letters A 135, no. 8-9 (1989): 458–60. http://dx.doi.org/10.1016/0375-9601(89)90048-0.

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26

Graham, M. K., J. G. McGeown, and I. Fairweather. "Ionic mechanisms underlying spontaneous muscle contractions in the liver fluke, Fasciola hepatica." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 277, no. 2 (1999): R374—R383. http://dx.doi.org/10.1152/ajpregu.1999.277.2.r374.

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Spontaneous contractions of liver fluke muscle were abolished in Ca2+-free saline and by 100 μM nifedipine and reduced by 5 mM cadmium chloride, suggesting that they are dependent on extracellular Ca2+. Caffeine (5 mM) significantly increased contraction amplitude and frequency. Ryanodine (100 μM) failed to block the caffeine response but significantly reduced spontaneous contraction frequency, suggesting that intracellular stores have a functional role. Cyclopiazonic acid (5 μM) had no effect on the caffeine response or spontaneous activity. 3-Isobutyl-1-methylxanthine (IBMX), forskolin, and

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27

Tutwiler, Valerie, Rustem I. Litvinov, Tatiana Lebedeva, Fazoil I. Ataullakhanov, Douglas B. Cines, and John W. Weisel. "Effects of Platelets and Erythrocytes on the Dynamic Size and Mechanical Properties of Blood Clots during Contraction." Blood 124, no. 21 (2014): 4225. http://dx.doi.org/10.1182/blood.v124.21.4225.4225.

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Abstract Clot contraction is a final step of blood clotting and plays a key role in hemostasis and restoring blood flow past obstructive thrombi. The volume shrinkage of clots is driven by the contractile forces generated by activated platelets and propagated by the platelet-attached viscoelastic fibrin fibers throughout the entire clot. We have recently shown that blood clot contraction results in the formation of compressed. tightly packed, polyhedral erythrocytes (polyhedrocytes) and in the redistribution of platelets and fibrin to the surface of the contracted clot as a result of the compl

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Stebbins, C. L. "Reflex cardiovascular response to exercise is modulated by circulating vasopressin." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 263, no. 5 (1992): R1104—R1109. http://dx.doi.org/10.1152/ajpregu.1992.263.5.r1104.

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Peripheral vasopressin (AVP) can act centrally to sensitize the arterial baroreflex and/or peripherally to attenuate regional blood flow by a direct vascular effect. Because plasma concentrations of AVP increase during exercise, this study examined the possibility that AVP is capable of modulating the reflex cardiovascular response to static muscle contraction. Thus, in anesthetized cats, the pressor [mean arterial pressure (MAP)], myocardial contractile (dP/dt), and heart rate responses to 30-45 s of electrically induced static contraction of the hindlimb muscles were compared before and afte

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Cortright, Ronald N., and G. Lynis Dohm. "Mechanisms by Which Insulin and Muscle Contraction Stimulate Glucose Transport." Canadian Journal of Applied Physiology 22, no. 6 (1997): 519–30. http://dx.doi.org/10.1139/h97-033.

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Insulin binding to its receptor activates a tyrosine kinase that initiates a cascade of signaling events, the initial step being the tyrosine phosphorylation of insulin receptor substrate 1 (IRS-1). Subsequent IRS-1 association and activation of phosphatidylinositiol 3-kinase (PI 3-kinase) is believed to be involved in the events leading to the translocation of glucose transporters (GLUT4) to the plasma membrane resulting in uptake of glucose into the cell. Muscle contractions increase insulin sensitivity, but also stimulate muscle glucose uptake independent of insulin. The contraction signali

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Ploug, T., H. Galbo, T. Ohkuwa, J. Tranum-Jensen, and J. Vinten. "Kinetics of glucose transport in rat skeletal muscle membrane vesicles: effects of insulin and contractions." American Journal of Physiology-Endocrinology and Metabolism 262, no. 5 (1992): E700—E711. http://dx.doi.org/10.1152/ajpendo.1992.262.5.e700.

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To study the mechanism of acceleration of glucose transport in skeletal muscle after stimulation with insulin and contractions, we isolated a subcellular vesicular membrane fraction, highly enriched in the plasma membrane enzyme K(+)-stimulated p-nitrophenylphosphatase and also enriched in some intracellular membranes. Protein recovery, morphology, lipid content, marker enzyme activities, total intravesicular volume, Western blot quantitation of GLUT-1, and glucose-inhibitable cytochalasin B binding were identical in membrane fractions from control, insulin-stimulated, contraction-stimulated,

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31

BUTLER, R. D., and C. R. McCROHAN. "Stimulus-response coupling in the contraction of tentacles of the suctorian protozoon Heliophrya erhardi." Journal of Cell Science 88, no. 1 (1987): 121–27. http://dx.doi.org/10.1242/jcs.88.1.121.

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Experiments were carried out to determine the role of the plasma membrane in the control of tentacle contraction in Heliophrya erhardi. Intracellular recordings gave membrane potentials between −20 and −30 mV. In a Ca2+-containing medium mechanical stimulation induced tentacle contraction but no associated electrical events were recorded. Intracellular stimulation with 50 nA, 100 ms hyperpolarizing current induced contraction, but no significant changes in membrane potential, whereas up to 100 nA, 100 ms depolarizing current had no effect. In a Ca2+-free medium neither mechanical stimulation n

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Jansen, Sepp R., Anna M. Van Ziel, Hoeke A. Baarsma та Reinoud Gosens. "β-Catenin regulates airway smooth muscle contraction". American Journal of Physiology-Lung Cellular and Molecular Physiology 299, № 2 (2010): L204—L214. http://dx.doi.org/10.1152/ajplung.00020.2010.

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β-Catenin is an 88-kDa member of the armadillo family of proteins that is associated with the cadherin-catenin complex in the plasma membrane. This complex interacts dynamically with the actin cytoskeleton to stabilize adherens junctions, which play a central role in force transmission by smooth muscle cells. Therefore, in the present study, we hypothesized a role for β-catenin in the regulation of smooth muscle force production. β-Catenin colocalized with smooth muscle α-actin (sm-α-actin) and N-cadherin in plasma membrane fractions and coimmunoprecipitated with sm-α-actin and N-cadherin in l

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Lopes-Martins, Rodrigo Álvaro B., Rodrigo Labat Marcos, Patrícia Sardinha Leonardo, et al. "Effect of low-level laser (Ga-Al-As 655 nm) on skeletal muscle fatigue induced by electrical stimulation in rats." Journal of Applied Physiology 101, no. 1 (2006): 283–88. http://dx.doi.org/10.1152/japplphysiol.01318.2005.

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We investigated whether low-level laser therapy (LLLT) can reduce muscular fatigue during tetanic contractions in rats. Thirty-two male Wistar rats were divided into four groups receiving either one of three different LLLT doses (0.5, 1.0, and 2.5 J/cm2) or a no-treatment control group. Electrical stimulation was used to induce six tetanic muscle contractions in the tibial anterior muscle. Contractions were stopped when the muscle force fell to 50% of the initial value for each contraction (T50%). There was no significant difference between the 2.5 J/cm2 laser-irradiated group and the control

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Xiao, Zuo-Liang, Qian Chen, Piero Biancani, and Jose Behar. "Abnormalities of gallbladder muscle associated with acute inflammation in guinea pigs." American Journal of Physiology-Gastrointestinal and Liver Physiology 281, no. 2 (2001): G490—G497. http://dx.doi.org/10.1152/ajpgi.2001.281.2.g490.

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Muscle strips from experimental acute cholecystitis (AC) exhibit a defective contraction. The mechanisms responsible for this impaired contraction are not known. The present studies investigated the nature of these abnormalities. AC was induced by ligating the common bile duct of guinea pigs for 3 days. Contraction was studied in enzymatic dissociated muscle cells. Cholecystokinin (CCK) and prostaglandin E2 (PGE2) receptor binding studies were performed by radioreceptor assay. The levels of lipid peroxidation, cholesterol, phospholipid, and H2O2 as well as the catalase and superoxide dismutase

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Chan, M. H. Stanley, Andrew L. Carey, Matthew J. Watt, and Mark A. Febbraio. "Cytokine gene expression in human skeletal muscle during concentric contraction: evidence that IL-8, like IL-6, is influenced by glycogen availability." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 287, no. 2 (2004): R322—R327. http://dx.doi.org/10.1152/ajpregu.00030.2004.

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To determine the expression and induction of cytokines in human skeletal muscle during concentric contractions, eight males performed 60 min of bicycle exercise, with either a normal (Con) or reduced (Lo Gly) preexercise intramuscular glycogen content. Muscle biopsy samples were obtained before and after exercise and analyzed for glycogen and the mRNA expression of 13 cytokines. Resting muscle glycogen was higher ( P < 0.05) in Con compared with Lo Gly and was reduced ( P < 0.05) to 102 ± 32 vs. 17 ± 5 mmol U glycosyl/kg dry mass for Con and Lo Gly, respectively. We detected mRNA levels

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Lau, R., W. D. Blinn, A. Bonen, and D. J. Dyck. "Stimulatory effects of leptin and muscle contraction on fatty acid metabolism are not additive." American Journal of Physiology-Endocrinology and Metabolism 281, no. 1 (2001): E122—E129. http://dx.doi.org/10.1152/ajpendo.2001.281.1.e122.

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Leptin has been shown to acutely stimulate fatty acid oxidation and triacylglycerol hydrolysis in skeletal muscle. These effects are similar to those induced by muscle contraction alone. Several studies have demonstrated that, during aerobic exercise, plasma leptin concentrations are well maintained; however, none has examined whether the stimulatory effects of leptin and contraction on muscle lipid metabolism are additive. This is the first study to examine the direct effect of leptin on lipid and carbohydrate (CHO) metabolism in isolated oxidative muscle over a range of contraction intensiti

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Kristiansen, S., J. Jones, A. Handberg, G. L. Dohm, and E. A. Richter. "Eccentric contractions decrease glucose transporter transcription rate, mRNA, and protein in skeletal muscle." American Journal of Physiology-Cell Physiology 272, no. 5 (1997): C1734—C1738. http://dx.doi.org/10.1152/ajpcell.1997.272.5.c1734.

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We have recently shown that eccentric contractions (ECs; forced lengthening of active muscle) elicit a delayed decrease in glucose transporter (GLUT-4) protein content in rat skeletal muscle and a decrease in subsequent contraction-stimulated glucose transport. Here, we investigate whether this decrease in total GLUT-4 protein after prior ECs is due to changes in GLUT-4 gene transcription rate and GLUT-4 mRNA level. Furthermore, the effect of prior ECs on sarcolemmal GLUT-4 protein content in plasma membrane (PM) vesicles isolated from contraction-stimulated muscle was determined. Rat gastrocn

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Viegas, P., L. Vialetto, A. J. Wolf, et al. "Insight into contraction dynamics of microwave plasmas for CO2 conversion from plasma chemistry modelling." Plasma Sources Science and Technology 29, no. 10 (2020): 105014. http://dx.doi.org/10.1088/1361-6595/abb41c.

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Wright, G., S. Wang, G. Bailey, V. Reichenbecher, and G. L. Wright. "Effect of retinoic acid on contractile competence of vascular smooth muscle." American Journal of Physiology-Heart and Circulatory Physiology 270, no. 4 (1996): H1363—H1370. http://dx.doi.org/10.1152/ajpheart.1996.270.4.h1363.

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Rat aortic rings cultured for 24 h in protein-free medium showed a significant reduction in the contractile response to potassium (-60%) and to phorbol 12,13-dibutyrate (-65%). The addition of plasma to the medium attenuated the loss in responsiveness, and the supplementation of the plasma-containing medium with all trans-retinoic acid (RA) returned the response to potassium (85%) and phorbol ester (135%) to near normal or supramaximal compared with fresh tissue. Furthermore, the combined additions of plasma and RA resulted in significant preservation of the contractile response (75%) for at l

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Fox, N. J., M. Lockwood, S. W. H. Cowley, et al. "EISCAT observations of unusual flows in the morning sector associated with weak substorm activity." Annales Geophysicae 12, no. 6 (1994): 541–53. http://dx.doi.org/10.1007/s00585-994-0541-2.

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Abstract. A discussion is given of plasma flows in the dawn and nightside high-latitude ionospheric regions during substorms occurring on a contracted auroral oval, as observed using the EISCAT CP-4-A experiment. Supporting data from the PACE radar, Greenland magnetometer chain, SAMNET magnetometers and geostationary satellites are compared to the EISCAT observations. On 4 October 1989 a weak substorm with initial expansion phase onset signatures at 0030 UT, resulted in the convection reversal boundary observed by EISCAT (at ~0415 MLT) contracting rapidly poleward, causing a band of elevated i

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Davy, K. P., W. G. Herbert, and J. H. Williams. "Effect of indomethacin on the pressor responses to sustained isometric contraction in humans." Journal of Applied Physiology 75, no. 1 (1993): 273–78. http://dx.doi.org/10.1152/jappl.1993.75.1.273.

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The purpose of this study was to test the hypothesis that prostaglandins participate in metaboreceptor stimulation of the pressor response to sustained isometric handgrip contraction in humans. To accomplish this, mean arterial pressure, heart rate (n = 10), and plasma norepinephrine levels (n = 8) were measured in healthy male subjects during sustained isometric handgrip at 40% of maximal voluntary contraction force to exhaustion and during a period of postcontraction muscle ischemia. The subjects were given a double-blind and counterbalanced administration of placebo or a single 100-mg dose

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Stavrou, Irene, and Theresa J. O'Halloran. "The Monomeric Clathrin Assembly Protein, AP180, Regulates Contractile Vacuole Size in Dictyostelium discoideum." Molecular Biology of the Cell 17, no. 12 (2006): 5381–89. http://dx.doi.org/10.1091/mbc.e06-06-0531.

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AP180, one of many assembly proteins and adaptors for clathrin, stimulates the assembly of clathrin lattices on membranes, but its unique contribution to clathrin function remains elusive. In this study we identified the Dictyostelium discoideum ortholog of the adaptor protein AP180 and characterized a mutant strain carrying a deletion in this gene. Imaging GFP-labeled AP180 showed that it localized to punctae at the plasma membrane, the contractile vacuole, and the cytoplasm and associated with clathrin. AP180 null cells did not display defects characteristic of clathrin mutants and continued

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Baines, I. C., A. Corigliano-Murphy, and E. D. Korn. "Quantification and localization of phosphorylated myosin I isoforms in Acanthamoeba castellanii." Journal of Cell Biology 130, no. 3 (1995): 591–603. http://dx.doi.org/10.1083/jcb.130.3.591.

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The actin-activated Mg(2+)-ATPase activities of the three myosin I isoforms in Acanthamoeba castellanii are significantly expressed only after phosphorylation of a single site in the myosin I heavy chain. Synthetic phosphorylated and unphosphorylated peptides corresponding to the phosphorylation site sequences, which differ for the three myosin I isoforms, were used to raise isoform-specific antibodies that recognized only the phosphorylated myosin I or the total myosin I isoform (phosphorylated and unphosphorylated), respectively. With these antisera, the amounts of total and phosphorylated i

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Crecelius, Anne R., Brett S. Kirby, Jennifer C. Richards, and Frank A. Dinenno. "Mechanical effects of muscle contraction increase intravascular ATP draining quiescent and active skeletal muscle in humans." Journal of Applied Physiology 114, no. 8 (2013): 1085–93. http://dx.doi.org/10.1152/japplphysiol.01465.2012.

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Intravascular adenosine triphosphate (ATP) evokes vasodilation and is implicated in the regulation of skeletal muscle blood flow during exercise. Mechanical stresses to erythrocytes and endothelial cells stimulate ATP release in vitro. How mechanical effects of muscle contractions contribute to increased plasma ATP during exercise is largely unexplored. We tested the hypothesis that simulated mechanical effects of muscle contractions increase [ATP]venous and ATP effluent in vivo, independent of changes in tissue metabolic demand, and further increase plasma ATP when superimposed with mild-inte

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Rabinovici, Jaron, Talma Rosenthal, Edna Peleg, and Shlomo Mashiach. "Changes in Plasma Norepinephrine in Normal Labor following Isometric Contraction." Gynecologic and Obstetric Investigation 23, no. 4 (1987): 267–70. http://dx.doi.org/10.1159/000298871.

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Kinoshita, K., Akihiko Hayashi, Kohzo Akahide, and Taketoshi Yamazaki. "High power plasma arc melting process for incinerated ash contraction." Pure and Applied Chemistry 66, no. 6 (1994): 1295–300. http://dx.doi.org/10.1351/pac199466061295.

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Kabouzi, Y., M. D. Calzada, M. Moisan, K. C. Tran, and C. Trassy. "Radial contraction of microwave-sustained plasma columns at atmospheric pressure." Journal of Applied Physics 91, no. 3 (2002): 1008–19. http://dx.doi.org/10.1063/1.1425078.

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Lee, Myung Gwon, Sungyoung Choi, Hee-Je Kim, et al. "Inertial blood plasma separation in a contraction–expansion array microchannel." Applied Physics Letters 98, no. 25 (2011): 253702. http://dx.doi.org/10.1063/1.3601745.

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Barroso, Renato, Hamilton Roschel, Carlos Ugrinowitsch, Rubens Araújo, Kazunori Nosaka, and Valmor Tricoli. "Effect of eccentric contraction velocity on muscle damage in repeated bouts of elbow flexor exercise." Applied Physiology, Nutrition, and Metabolism 35, no. 4 (2010): 534–40. http://dx.doi.org/10.1139/h10-042.

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Eccentric exercise induces muscle damage, but controversy exists concerning the effect of contraction velocity on the magnitude of muscle damage, and little is known about the effect of contraction velocity on the repeated-bout effect. This study examined slow (60°·s–1) and fast (180°·s–1) velocity eccentric exercises for changes in indirect markers of muscle damage following 3 exercise bouts that were performed every 2 weeks. Fifteen young men were divided into 2 groups based on the velocity of eccentric exercise: 7 in the Ecc60 (60°·s–1) group, and 8 in the Ecc180 (180°·s–1) group. The exerc

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Stojic, Marija, Joaquín Ródenas-Rochina, María Luisa López-Donaire, et al. "Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering." Polymers 13, no. 13 (2021): 2114. http://dx.doi.org/10.3390/polym13132114.

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Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been extensively studied for skin engineering. However, they showed rapid degradation and contraction over time and low mechanical properties which limit their reproducibility and lifespan. In order to achieve better mechanical properties, elasticity and biological properties, we incorporated a elastin-like recombinamer (ELR) network, based on two types of ELR, one modified with azide (SKS-N3) and other with cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 at three different SKS (serine-lysine-serine sequence)

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