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Journal articles on the topic 'Plasmi freddi'

Author: Grafiati

Published: 9 March 2023

Last updated: 31 July 2025

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1

Acosta-Jurado, Sebastián, Cynthia Alías-Villegas, Andrés Almozara, M. Rosario Espuny, José-María Vinardell, and Francisco Pérez-Montaño. "Deciphering the Symbiotic Significance of Quorum Sensing Systems of Sinorhizobium fredii HH103." Microorganisms 8, no. 1 (2020): 68. http://dx.doi.org/10.3390/microorganisms8010068.

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Quorum sensing (QS) is a bacterial cell-to-cell signaling mechanism that collectively regulates and synchronizes behaviors by means of small diffusible chemical molecules. In rhizobia, QS systems usually relies on the synthesis and detection of N-acyl-homoserine lactones (AHLs). In the model bacterium Sinorhizobium meliloti functions regulated by the QS systems TraI-TraR and SinI-SinR(-ExpR) include plasmid transfer, production of surface polysaccharides, motility, growth rate and nodulation. These systems are also present in other bacteria of the Sinorhizobium genus, with variations at the sp

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Vinardell, JoséMaría, Francisco Javier Ollero, Ángeles Hidalgo, et al. "NolR Regulates Diverse Symbiotic Signals of Sinorhizobium fredii HH103." Molecular Plant-Microbe Interactions® 17, no. 6 (2004): 676–85. http://dx.doi.org/10.1094/mpmi.2004.17.6.676.

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We have investigated in Sinorhizobium fredii HH103-1 (=HH103 Strr) the influence of the nolR gene on the production of three different bacterial symbiotic signals: Nod factors, signal responsive (SR) proteins, and exopolysac-charide (EPS). The presence of multiple copies of nolR (in plasmid pMUS675) repressed the transcription of all the flavonoid-inducible genes analyzed: nodA, nodD1, nolO, nolX, noeL, rhcJ, hesB, and y4pF. Inactivation of nolR (mutant SVQ517) or its overexpression (presence of pMUS675) altered the amount of Nod factors detected. Mutant SVQ517 produced Nod factors carrying N-

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3

Dave, Akash, Tanvi Khanna, and Pushpa Robin. "Exploiting Rhizobium for Cadmium Sulphide Nanoparticle Synthesis: Heterologous Expression of an Escherichia coli DH10B Enzyme, YbdK [EC: 6.3.2.2] in Sinorhizobium fredii NGR234." Journal of Pure and Applied Microbiology 16, no. 1 (2022): 593–605. http://dx.doi.org/10.22207/jpam.16.1.59.

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Escherichia coli DH10B has 1.1 kb ybdK gene which is responsible for encoding YbdK enzyme that possess a Gamma glutamyl cysteine synthetase activity. ybdK gene was ligated downstream of a constitutive derepressed lac promoter of a low copy number plasmid vector pBBR1MCS-2, giving rise to a recombinant plasmid pPAT. Sinorhizobium fredii NGR234 transformed with pPAT showed an augmented production of glutathione which in turn increased the production of cadmium sulphide nanoparticles to some extent. Also, a heterologous expression of YbdK in Sinorhizobium fredii NGR234 improved the oxidation stat

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Jiang, Guoqiao, and Hari B. Krishnan. "Sinorhizobium fredii USDA257, a Cultivar-Specific Soybean Symbiont, Carries Two Copies of y4yA and y4yB, Two Open Reading Frames That Are Located in a Region That Encodes the Type III Protein Secretion System." Molecular Plant-Microbe Interactions® 13, no. 9 (2000): 1010–14. http://dx.doi.org/10.1094/mpmi.2000.13.9.1010.

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Sinorhizobium fredii USDA257 forms nitrogen-fixing nodules on primitive soybean (Glycine max) cultivar Peking but fails to nodulate the improved cultivar McCall. Cultivar specificity is governed by a plasmid-borne locus, nolXBTUV. By DNA sequence analysis, we have identified two open reading frames, y4yA and y4yB, immediately downstream of nolX. Northern (RNA) blot analysis indicated that the expression of both y4yA and y4yB is inducible by isoflavonoids, and an intact copy of nolX is required. Two copies each of y4yA and y4yB are present in S. fredii USDA257, one on the sym plasmid (y4yAsp an

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Vinardell, José-María, Sebastián Acosta-Jurado, Susanne Zehner, et al. "The Sinorhizobium fredii HH103 Genome: A Comparative Analysis With S. fredii Strains Differing in Their Symbiotic Behavior With Soybean." Molecular Plant-Microbe Interactions® 28, no. 7 (2015): 811–24. http://dx.doi.org/10.1094/mpmi-12-14-0397-fi.

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Sinorhizobium fredii HH103 is a fast-growing rhizobial strain infecting a broad range of legumes including both American and Asiatic soybeans. In this work, we present the sequencing and annotation of the HH103 genome (7.25 Mb), consisting of one chromosome and six plasmids and representing the structurally most complex sinorhizobial genome sequenced so far. Comparative genomic analyses of S. fredii HH103 with strains USDA257 and NGR234 showed that the core genome of these three strains contains 4,212 genes (61.7% of the HH103 genes). Synteny plot analysis revealed that the much larger chromos

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6

Hashem, F. M., L. D. Kuykendall, S. E. Udell, and P. M. Thomas. "Phage susceptibility and plasmid profile analysis of Sinorhizobium fredii." Plant and Soil 186, no. 1 (1996): 127–34. http://dx.doi.org/10.1007/bf00035066.

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7

Krishnan, Hari B., Won-Seok Kim, Jeong Sun-Hyung, Kil Yong Kim, and Guoqiao Jiang. "Citrate Synthase Mutants of Sinorhizobium fredii USDA257 Form Ineffective Nodules with Aberrant Ultrastructure." Applied and Environmental Microbiology 69, no. 6 (2003): 3561–68. http://dx.doi.org/10.1128/aem.69.6.3561-3568.2003.

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ABSTRACT The tricarboxylic acid (TCA) cycle plays an important role in generating the energy required by bacteroids to fix atmospheric nitrogen. Citrate synthase is the first enzyme that controls the entry of carbon into the TCA cycle. We cloned and determined the nucleotide sequence of the gltA gene that encodes citrate synthase in Sinorhizobium fredii USDA257, a symbiont of soybeans (Glycine max [L.] Merr.) and several other legumes. The deduced citrate synthase protein has a molecular weight of 48,198 and exhibits sequence similarity to citrate synthases from several bacterial species, incl

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8

Jiao, Jian, Li Juan Wu, Biliang Zhang, et al. "MucR Is Required for Transcriptional Activation of Conserved Ion Transporters to Support Nitrogen Fixation of Sinorhizobium fredii in Soybean Nodules." Molecular Plant-Microbe Interactions® 29, no. 5 (2016): 352–61. http://dx.doi.org/10.1094/mpmi-01-16-0019-r.

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To achieve effective symbiosis with legume, rhizobia should fine-tune their background regulation network in addition to activating key genes involved in nodulation (nod) and nitrogen fixation (nif). Here, we report that an ancestral zinc finger regulator, MucR1, other than its paralog, MucR2, carrying a frameshift mutation, is essential for supporting nitrogen fixation of Sinorhizobium fredii CCBAU45436 within soybean nodules. In contrast to the chromosomal mucR1, mucR2 is located on symbiosis plasmid, indicating its horizontal transfer potential. A MucR2 homolog lacking the frameshift mutati

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9

Brom, Susana, Lourdes Girard, Alejandro García-de los Santos, Julio M. Sanjuan-Pinilla, José Olivares, and Juan Sanjuan. "Conservation of Plasmid-Encoded Traits among Bean-Nodulating Rhizobium Species." Applied and Environmental Microbiology 68, no. 5 (2002): 2555–61. http://dx.doi.org/10.1128/aem.68.5.2555-2561.2002.

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ABSTRACT Rhizobium etli type strain CFN42 contains six plasmids. We analyzed the distribution of genetic markers from some of these plasmids in bean-nodulating strains belonging to different species (Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum, and Sinorhizobium fredii). Our results indicate that independent of geographic origin, R. etli strains usually share not only the pSym plasmid but also other plasmids containing symbiosis-related genes, with a similar organization. In contrast, strains belonging to other bean-nodulating species seem to have acquired

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Ramakrishnan, Neela, R. K. Prakash, and Alan G. Atherly. "Conservation of IS66 homologue of octopine Ti plasmid DNA in Rhizobium fredii plasmid DNA." Plant Molecular Biology 7, no. 3 (1986): 177–88. http://dx.doi.org/10.1007/bf00021329.

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11

Mathis, James N., W. Mark Barbour, and Gerald H. Elkan. "Effect of Sym Plasmid Curing on Symbiotic Effectiveness in Rhizobium fredii†." Applied and Environmental Microbiology 49, no. 6 (1985): 1385–88. http://dx.doi.org/10.1128/aem.49.6.1385-1388.1985.

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12

FUDI, LI, CHEN WENLI, and ZHOU JUNCHU. "Variation of Sym Plasmid and Symbiotic Effectiveness in Isolates of Rhizobium fredii." Biodiversity Science 03, Suppl1 (1995): 36–42. http://dx.doi.org/10.17520/biods.1995042.

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13

Barbour, W. M., J. N. Mathis, and G. H. Elkan. "Evidence for plasmid- and chromosome-borne multiple nif genes in Rhizobium fredii." Applied and Environmental Microbiology 50, no. 1 (1985): 41–44. http://dx.doi.org/10.1128/aem.50.1.41-44.1985.

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14

Noreen, Sadaf, Helmi R. M. Schlaman, Ramón A. Bellogín, et al. "Alfalfa nodulation by Sinorhizobium fredii does not require sulfated Nod-factors." Functional Plant Biology 30, no. 12 (2003): 1219. http://dx.doi.org/10.1071/fp03093.

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Rhizobium strain 042B(s) is able to nodulate both soybean and alfalfa cultivars. We have demonstrated, by mass spectrometry, that the nodulation (Nod) factors produced by this strain are characteristic of those produced by Sinorhizobium fredii, which typically nodulates soybean; they have 3–5 N-acetylglucosamine (GlcNAc) residues, a mono-unsaturated or saturated C16, C18 or C20 fatty-acyl chain, and a (methyl)fucosyl residue on C6 of the reducing-terminal GlcNAc. In order to study Rhizobium strain 042B(s) and its nodulation behaviour further, we introduced an insertion mutation in the noeL gen

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15

Cubo, Teresa, Francisco Romero, Jose M. Vinardell, and Jose E. Ruiz-Sainz. "Expression of the Rhizobium leguminosarum biovar phaseoli melA Gene in Other Rhizobia Does Not Require the Presence of the nifA Gene." Functional Plant Biology 24, no. 2 (1997): 195. http://dx.doi.org/10.1071/pp96076.

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Many different Rhizobium strains produce melanin (Mel+) when grown on solid media supplemented with L-tyrosine. The composition of the media and the culture conditions are of great importance for pigment production. Previous reports showed that some Rhizobium leguminosarum biovar phaseoli strains that produce the pigment in complete solid media (TY) failed to produce the pigment in minimal media (SY) supplemented with L-tyrosine or in TY liquid media. In this paper we have investigated different R. fredii, R. meliloti, R. etli and R. leguminosarum bv. trifolii and phaseoli strains (all of them

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García-de los Santos, Alejandro, та Susana Brom. "Characterization of Two Plasmid-borne lpsβ Loci of Rhizobium etli Required for Lipopolysaccharide Synthesis and for Optimal Interaction with Plants". Molecular Plant-Microbe Interactions® 10, № 7 (1997): 891–902. http://dx.doi.org/10.1094/mpmi.1997.10.7.891.

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In Rhizobium etli CFN42, both the symbiotic plasmid (pd) and plasmid b (pb) are required for effective bean nodulation. This is due to the presence on pb of a region (lpsβ) involved in lipopolysaccharide (LPS) biosynthesis. We report here the genetic array and functional features of this plasmid-borne region. The sequence analysis of a 3,595-bp fragment revealed the presence of a transcriptional unit integrated by two open reading frames (lpsβ1 and lpsβ2) essential for LPS biosynthesis and symbiosis. The lpsβ1 encodes a putative 193 amino acid polypeptide that shows strong homology with glucos

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17

Richaume, A., J. S. Angle, and M. J. Sadowsky. "Influence of soil variables on in situ plasmid transfer from Escherichia coli to Rhizobium fredii." Applied and Environmental Microbiology 55, no. 7 (1989): 1730–34. http://dx.doi.org/10.1128/aem.55.7.1730-1734.1989.

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18

Miao, Lihong, Kui Zhou, Junchu Zhou, Dasong Chen, and Fuli Xie. "Apparent incompatibility of plasmid pSfrYC4b of Sinorhizobium fredii with two different plasmids in another strain." Archives of Microbiology 183, no. 5 (2005): 359–67. http://dx.doi.org/10.1007/s00203-005-0780-y.

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19

Cervantes, Laura, Fabiola Miranda-Sánchez, Gonzalo Torres Tejerizo, David Romero, and Susana Brom. "Plasmid pSfr64a and the symbiotic plasmid pSfr64b of Sinorhizobium fredii GR64 control each other's conjugative transfer through quorum-sensing elements." Plasmid 106 (November 2019): 102443. http://dx.doi.org/10.1016/j.plasmid.2019.102443.

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20

Machado, Daphne, Steven G. Pueppke, José Maria Vinardel, José E. Ruiz-Sainz, and Hari B. Krishnan. "Expression of nodD1 and nodD2 in Sinorhizobium fredii, a Nitrogen-Fixing Symbiont of Soybean and Other Legumes." Molecular Plant-Microbe Interactions® 11, no. 5 (1998): 375–82. http://dx.doi.org/10.1094/mpmi.1998.11.5.375.

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The ability of Sinorhizobium fredii strains USDA191 and USDA257 to form nitrogen-fixing root nodules on legume plants is regulated by nodD1 and nodD2, which sense flavonoid signals from host roots and then activate the expression of inducible nodulation genes. We assessed the interactions between these two loci with nodD1-negative and nodD2-negative mutants and with strains containing extra copies of these genes. Although both nodD1 and nodD2 are expressed constitutively, levels of nodD1 are much higher, as measured by RNA dot blots. Extra plasmid-borne copies of nodD2 reduced transcription of

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21

Lamrabet, Youssef, Ramón A. Bellogín, Teresa Cubo, et al. "Mutation in GDP-Fucose Synthesis Genes of Sinorhizobium fredii Alters Nod Factors and Significantly Decreases Competitiveness to Nodulate Soybeans." Molecular Plant-Microbe Interactions® 12, no. 3 (1999): 207–17. http://dx.doi.org/10.1094/mpmi.1999.12.3.207.

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We mutagenized Sinorhizobium fredii HH103-1 with Tn5- B20 and screened about 2,000 colonies for increased β-galactosidase activity in the presence of the flavonoid naringenin. One mutant, designated SVQ287, produces lipochitooligosaccharide Nod factors (LCOs) that differ from those of the parental strain. The nonreducing N-acetylglucosamine residues of all of the LCOs of mutant SVQ287 lack fucose and 2-O-methylfucose substituents. In addition, SVQ287 synthesizes an LCO with an unusually long, C20:1 fatty acyl side chain. The transposon insertion of mutant SVQ287 lies within a 1.1-kb HindIII fr

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Buendia-Claveria, Ana M., Francisco Romero, Teresa Cubo, Julio Perez-Silva, and Jose E. Ruiz-Sainz. "Inter and Intraspecific Transfer of a Rhizobium fredii Symbiotic Plasmid: Expression and Incompatibility of Symbiotic Plasmids." Systematic and Applied Microbiology 12, no. 2 (1989): 210–15. http://dx.doi.org/10.1016/s0723-2020(89)80016-5.

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Buendia-Claveria, Ana M., Manuel Chamber, and Jose E. Ruiz-Sainz. "A Comparative Study of the Physiological Characteristics, Plasmid Content and Symbiotic Properties of Different Rhizobium fredii Strains." Systematic and Applied Microbiology 12, no. 2 (1989): 203–9. http://dx.doi.org/10.1016/s0723-2020(89)80015-3.

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Lorio, Julio C., Demosthenis Chronis, and Hari B. Krishnan. "y4xP, an Open Reading Frame Located in a Type III Protein Secretion System Locus of Sinorhizobium fredii USDA257 and USDA191, Encodes Cysteine Synthase." Molecular Plant-Microbe Interactions® 19, no. 6 (2006): 635–43. http://dx.doi.org/10.1094/mpmi-19-0635.

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Sinorhizobium fredii USDA257, a soybean symbiont, exports several nodulation outer proteins (Nops) into the rhizo-sphere. These proteins, which are exported by a type III secretion system (TTSS), have a pivotal role in host-specific nodulation. The entire TTSS of S. fredii lies within a 31-kb region that includes conserved genes that code for secretion machinery proteins, Nops, and several open reading frames (ORF) of unknown function. Identifying the functions of these ORF is essential to understand fully the role of TTSS in nodulation. Here, we report the characterization of y4xP, an ORF of

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Shantharam, S., Kim S. Engwall, and Alan G. Atherly. "Symbiotic Phenotypes of Soybean Root Nodules Associated with Deletions and Rearrangements in the Symbiotic Plasmid of R. fredii USDA191." Journal of Plant Physiology 132, no. 4 (1988): 431–38. http://dx.doi.org/10.1016/s0176-1617(88)80057-9.

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Lorio, Julio C., Won Seok Kim, and Hari B. Krishnan. "NopB, a Soybean Cultivar-Specificity Protein from Sinorhizobium fredii USDA257, Is a Type III Secreted Protein." Molecular Plant-Microbe Interactions® 17, no. 11 (2004): 1259–68. http://dx.doi.org/10.1094/mpmi.2004.17.11.1259.

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The type III secretion system (TTSS) of plant- and animal-pathogenic bacteria is involved in translocation of virulence factors into the host cell cytosol where they modulate cellular processes. Sinorhizobium fredii USDA257 is a gram-negative soil bacterium that forms nitrogen-fixing nodules on specific soybean cultivars (Glycine max (L.) Merr.). This microsymbiont is known to secrete at least five nodulation outer proteins (Nops) in response to flavonoid induction. Some of these Nops have been shown to be secreted by TTSS in this symbiotic bacterium. We have isolated and purified an 18-kDa ex

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Unay, Jovelyn, and Xavier Perret. "A Minimal Genetic Passkey to Unlock Many Legume Doors to Root Nodulation by Rhizobia." Genes 11, no. 5 (2020): 521. http://dx.doi.org/10.3390/genes11050521.

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In legume crops, formation of developmentally mature nodules is a prerequisite for efficient nitrogen fixation by populations of rhizobial bacteroids established inside nodule cells. Development of root nodules, and concomitant microbial colonization of plant cells, are constrained by sets of recognition signals exchanged by infecting rhizobia and their legume hosts, with much of the specificity of symbiotic interactions being determined by the flavonoid cocktails released by legume roots and the strain-specific nodulation factors (NFs) secreted by rhizobia. Hence, much of Sinorhizobium fredii

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Dorigo, Ermes. "Il rapporto di amicizia tra Pietro Metastasio e il conte udinese Daniele Florio attraverso l'Epistolario del «poeta cesareo»." Forum Italicum: A Journal of Italian Studies 46, no. 1 (2012): 110–40. http://dx.doi.org/10.1177/001458581204600105.

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Questo scritto utilizza essenzialmente l' Epistolario di Pietro Metastasio (1698–1782), per analizzare il suo rapporto di amicizia col «ciambellano imperial cesareo» di Maria Teresa, il conte udinese Daniele Florio (1710–1789), «poeta de' Sovrani, Sovrano de' poeti», lungo tre direttrici adeguatamente approfondite. Amicizia come reciproco sostegno e guida, fraterna paterna filiale, a seconda dei casi, che comprende e travalica sincerità e onestà, di cui pur si nutre, per rasentare, con la tenerezza e l'affetto che la plasma, un sentimento di puro amore e disinteressata dedizione. Il tema dell'

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Ausmees, Nora, Hajime Kobayashi, William J. Deakin, et al. "Characterization of NopP, a Type III Secreted Effector of Rhizobium sp. Strain NGR234." Journal of Bacteriology 186, no. 14 (2004): 4774–80. http://dx.doi.org/10.1128/jb.186.14.4774-4780.2004.

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ABSTRACT The type three secretion system (TTSS) encoded by pNGR234a, the symbiotic plasmid of Rhizobium sp. strain NGR234, is responsible for the flavonoid- and NodD1-dependent secretion of n odulation o uter p roteins (Nops). Abolition of secretion of all or specific Nops significantly alters the nodulation ability of NGR234 on many of its hosts. In the closely related strain Rhizobium fredii USDA257, inactivation of the TTSS modifies the host range of the mutant so that it includes the improved Glycine max variety McCall. To assess the impact of individual TTSS-secreted proteins on symbioses

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Meinhardt, L. W., H. B. Krishnan, P. A. Balatti, and S. G. Pueppke. "Molecular cloning and characterization of a sym plasmid locus that regulates cultivar-specific nodulation of soybean by Rhizobium fredii USDA257." Molecular Microbiology 9, no. 1 (1993): 17–29. http://dx.doi.org/10.1111/j.1365-2958.1993.tb01665.x.

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Berck, S., X. Perret, D. Quesada-Vincens, J. C. Promé, W. J. Broughton, and S. Jabbouri. "NolL of Rhizobium sp. Strain NGR234 Is Required for O-Acetyltransferase Activity." Journal of Bacteriology 181, no. 3 (1999): 957–64. http://dx.doi.org/10.1128/jb.181.3.957-964.1999.

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ABSTRACT Following (iso)flavonoid induction, nodulation genes of the symbiotic nitrogen-fixing bacterium Rhizobium sp. strain NGR234 elaborate a large family of lipooligosaccharidic Nod factors (NodNGR factors). When secreted into the rhizosphere of compatible legumes, these signal molecules initiate root hair deformation and nodule development. The nonreducing glucosamine residue of NodNGR factors are N acylated, N methylated, and mono- or biscarbamoylated, while position C-6 of the reducing extremity is fucosylated. This fucose residue is normally 2-O methylated and either sulfated or acetyl

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Krishnan, Hari B., Julio Lorio, Won Seok Kim, et al. "Extracellular Proteins Involved in Soybean Cultivar-Specific Nodulation Are Associated with Pilus-Like Surface Appendages and Exported by a Type III Protein Secretion System in Sinorhizobium fredii USDA257." Molecular Plant-Microbe Interactions® 16, no. 7 (2003): 617–25. http://dx.doi.org/10.1094/mpmi.2003.16.7.617.

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Several gram-negative plant and animal pathogenic bacteria have evolved a type III secretion system (TTSS) to deliver effector proteins directly into the host cell cytosol. Sinorhizobium fredii USDA257, a symbiont of soybean and many other legumes, secretes proteins called Nops (nodulation outer proteins) into the extracellular environment upon flavonoid induction. Mutation analysis and the nucleotide sequence of a 31.2-kb symbiosis (sym) plasmid DNA region of USDA257 revealed the existence of a TTSS locus in this symbiotic bacterium. This locus includes rhc (rhizobia conserved) genes that enc

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Fumeaux, Coralie, Nadia Bakkou, Joanna Kopcińska, et al. "Functional analysis of the nifQdctA1y4vGHIJ operon of Sinorhizobium fredii strain NGR234 using a transposon with a NifA-dependent read-out promoter." Microbiology 157, no. 10 (2011): 2745–58. http://dx.doi.org/10.1099/mic.0.049999-0.

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Rhizobia are a disparate collection of soil bacteria capable of reducing atmospheric nitrogen in symbiosis with legumes (Fix phenotype). Synthesis of the nitrogenase and its accessory components is under the transcriptional control of the key regulator NifA and is generally restricted to the endosymbiotic forms of rhizobia known as bacteroids. Amongst studied rhizobia, Sinorhizobium fredii strain NGR234 has the remarkable ability to fix nitrogen in association with more than 130 species in 73 legume genera that form either determinate, indeterminate or aeschynomenoid nodules. Hence, NGR234 is

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Cervantes, Laura, Patricia Bustos, Lourdes Girard, et al. "The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobium strain." BMC Microbiology 11, no. 1 (2011): 149. http://dx.doi.org/10.1186/1471-2180-11-149.

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35

Krysciak, Dagmar, Jessica Grote, Mariita Rodriguez Orbegoso, et al. "RNA Sequencing Analysis of the Broad-Host-Range Strain Sinorhizobium fredii NGR234 Identifies a Large Set of Genes Linked to Quorum Sensing-Dependent Regulation in the Background of atraIandngrIDeletion Mutant." Applied and Environmental Microbiology 80, no. 18 (2014): 5655–71. http://dx.doi.org/10.1128/aem.01835-14.

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ABSTRACTThe alphaproteobacteriumSinorhizobium frediiNGR234 has an exceptionally wide host range, as it forms nitrogen-fixing nodules with more legumes than any other known microsymbiont. Within its 6.9-Mbp genome, it encodes twoN-acyl-homoserine-lactone synthase genes (i.e.,traIandngrI) involved in the biosynthesis of two distinct autoinducer I-type molecules. Here, we report on the construction of an NGR234-ΔtraIand an NGR234-ΔngrImutant and their genome-wide transcriptome analysis. A high-resolution RNA sequencing (RNA-seq) analysis of early-stationary-phase cultures in the NGR234-ΔtraIbackg

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Rasmussen, Hans Christian, Maiken Stilling, Johanne Gade Lilleøre, et al. "BONE AND SOFT-TISSUE CONCENTRATIONS OF PENICILLIN: IS ORAL PENICILLIN V NON-INFERIOR TO INTRAVENOUS PENICILLIN G?" Orthopaedic Proceedings 105-B, SUPP_17 (2023): 43. http://dx.doi.org/10.1302/1358-992x.2023.17.043.

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AimThe β-lactam penicillin is often used in the treatment of soft tissue infections and osteomyelitis caused by penicillin susceptible Staphylococcus aureus. Oral antibiotic treatment has been shown to be non-inferior to intravenous (IV) therapy when used during the first 6 weeks in complex orthopedic infections (OVIVA trial). However, the use of oral β-lactams in osteomyelitis treatment remains a topic of debate due to low and variable bioavailability. The aim was to assess the time for which the unbound penicillin concentration exceeded targeted minimum inhibitory concentrations (fT>MIC)

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Sun, Yan-Wei, Yan Li, Yue Hu, Wen-Xin Chen, and Chang-Fu Tian. "Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234." Applied and Environmental Microbiology 85, no. 19 (2019). http://dx.doi.org/10.1128/aem.00717-19.

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ABSTRACT The exact roles of various granule-associated proteins (GAPs) of polyhydroxybutyrate (PHB) are poorly investigated, particularly for bacteria associated with plants. In this study, four structural GAPs, named phasins PhaP1 to PhaP4, were identified and demonstrated as true phasins colocalized with PHB granules in Sinorhizobium fredii NGR234, a facultative microsymbiont of Vigna unguiculata and many other legumes. The conserved PhaP2 dominated in regulation of granule size under both free-living and symbiotic conditions. PhaP1, another conserved phasin, made a higher contribution than

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Shi, Wen-Tao, Biliang Zhang, Meng-Lin Li, Ke-Han Liu, Jian Jiao та Chang-Fu Tian. "The convergent xenogeneic silencer MucR predisposes α-proteobacteria to integrate AT-rich symbiosis genes". Nucleic Acids Research, 4 серпня 2022. http://dx.doi.org/10.1093/nar/gkac664.

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Abstract Bacterial adaptation is largely shaped by horizontal gene transfer, xenogeneic silencing mediated by lineage-specific DNA bridgers (H-NS, Lsr2, MvaT and Rok), and various anti-silencing mechanisms. No xenogeneic silencing DNA bridger is known for α-proteobacteria, from which mitochondria evolved. By investigating α-proteobacterium Sinorhizobium fredii, a facultative legume microsymbiont, here we report the conserved zinc-finger bearing MucR as a novel xenogeneic silencing DNA bridger. Self-association mediated by its N-terminal domain (NTD) is required for DNA–MucR–DNA bridging comple

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Barbour, W. Mark, and G. H. Elkan. "Physiological characteristics and competitive ability of plasmid-cured derivatives of Rhizobium fredii USDA 206." Archives of Microbiology 154, no. 1 (1990). http://dx.doi.org/10.1007/bf00249169.

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Grote, Jessica, Dagmar Krysciak, Katrin Petersen, et al. "The Absence of the N-acyl-homoserine-lactone Autoinducer Synthase Genes traI and ngrI Increases the Copy Number of the Symbiotic Plasmid in Sinorhizobium fredii NGR234." Frontiers in Microbiology 7 (November 18, 2016). http://dx.doi.org/10.3389/fmicb.2016.01858.

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