Academic literature on the topic 'Plasmid cloning vectors'

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Journal articles on the topic "Plasmid cloning vectors"

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Green, Michael R., and Joseph Sambrook. "Cloning in Plasmid Vectors: Directional Cloning." Cold Spring Harbor Protocols 2020, no. 11 (2020): pdb.prot101238. http://dx.doi.org/10.1101/pdb.prot101238.

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Green, Michael R., and Joseph Sambrook. "Cloning in Plasmid Vectors: Blunt-End Cloning." Cold Spring Harbor Protocols 2020, no. 11 (2020): pdb.prot101246. http://dx.doi.org/10.1101/pdb.prot101246.

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Sambrook, Joseph, and David W. Russell. "Directional Cloning into Plasmid Vectors." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot3919. http://dx.doi.org/10.1101/pdb.prot3919.

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Burbank, Lindsey P., and Drake C. Stenger. "Plasmid Vectors for Xylella fastidiosa Utilizing a Toxin-Antitoxin System for Stability in the Absence of Antibiotic Selection." Phytopathology® 106, no. 8 (2016): 928–36. http://dx.doi.org/10.1094/phyto-02-16-0097-r.

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The phytopathogen Xylella fastidiosa causes disease in a variety of important crop and landscape plants. Functional genetic studies have led to a broader understanding of virulence mechanisms used by this pathogen in the grapevine host. Plasmid shuttle vectors are important tools in studies of bacterial genetics but there are only a limited number of plasmid vectors available that replicate in X. fastidiosa, and even fewer that are retained without antibiotic selection. Two plasmids are described here that show stable replication in X. fastidiosa and are effective for gene complementation both
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Farkas, Joel, Daehwan Chung, Megan DeBarry, Michael W. W. Adams, and Janet Westpheling. "Defining Components of the Chromosomal Origin of Replication of the Hyperthermophilic Archaeon Pyrococcus furiosus Needed for Construction of a Stable Replicating Shuttle Vector." Applied and Environmental Microbiology 77, no. 18 (2011): 6343–49. http://dx.doi.org/10.1128/aem.05057-11.

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ABSTRACTWe report the construction of a series of replicating shuttle vectors that consist of a low-copy-number cloning vector forEscherichia coliand functional components of the origin of replication (oriC) of the chromosome of the hyperthermophilic archaeonPyrococcus furiosus. In the process of identifying the minimum replication origin sequence required for autonomous plasmid replication inP. furiosus, we discovered that several features of the origin predicted by bioinformatic analysis andin vitrobinding studies were not essential for stable autonomous plasmid replication. A minimum region
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Dombrecht, Bruno, Jos Vanderleyden, and Jan Michiels. "Stable RK2-Derived Cloning Vectors for the Analysis of Gene Expression and Gene Function in Gram-Negative Bacteria." Molecular Plant-Microbe Interactions® 14, no. 3 (2001): 426–30. http://dx.doi.org/10.1094/mpmi.2001.14.3.426.

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The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoter-less gusA gene are suitable for the study of gene function or regulation in bacteria.
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Sambrook, Joseph, and David W. Russell. "Blunt-ended Cloning into Plasmid Vectors." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot3921. http://dx.doi.org/10.1101/pdb.prot3921.

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King, Kendall W., and Kevin Dybvig. "Mycoplasmal Cloning Vectors Derived from Plasmid pKMK1." Plasmid 31, no. 1 (1994): 49–59. http://dx.doi.org/10.1006/plas.1994.1006.

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Élő, P., Sz Semsey, A. Kereszt, T. Nagy, P. Papp, and L. Orosz. "Integrative promoter cloning plasmid vectors forRhizobium meliloti." FEMS Microbiology Letters 159, no. 1 (1998): 7–13. http://dx.doi.org/10.1111/j.1574-6968.1998.tb12834.x.

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Heeb, Stephan, Yoshifumi Itoh, Takayuki Nishijyo, et al. "Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria." Molecular Plant-Microbe Interactions® 13, no. 2 (2000): 232–37. http://dx.doi.org/10.1094/mpmi.2000.13.2.232.

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The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
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Dissertations / Theses on the topic "Plasmid cloning vectors"

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Jeong, Pyengsoo. "Construction of a Cloning Vector Based upon a Rhizobium Plasmid Origin of Replication and its Application to Genetic Engineering of Rhizobium Strains." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc278189/.

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Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots. Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium species.
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Nampaisansuk, Mongkol. "Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants". Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3123/.

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A cotton PATE cDNA clone has a 1.7-kb insert with an coding region for 410 amino acids, lacking codons for the three N-terminal amino acids. The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The gene spans 3.6 kb with six exons and five introns. The six exons are identical in nucleotide sequence to the open reading frame of the corresponding cDNA, and
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Snyder, William E. "Construction of a hybrid vector which allows for regulation of expression of cloned genes in anacystis nidulans R2 by controlling the iron content of the growth medium." Virtual Press, 1989. http://liblink.bsu.edu/uhtbin/catkey/560278.

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A hybrid vector, pANIC1, was to be constructed which was capable of regulating expression of cloned genes in both Escherichia coli and Anacystis nidulans R2 by controlling the iron content of the growth medium. Plasmid pANIC1 would have origins of replication for E. coli and A. nidulans R2, and a marker gene conferring ampicillin resistance. It would also contain the promoter for the irpA gene which is active only in low iron growth conditions.The first two stages of the construction were successfully completed, but unfortunately the final construction proved to be unstable. Recent information
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Blatch, Laura. "Evaluation of a marker gene operon for plastid transformation and cloning of genes encoding cell wall degrading enzymes in plastid transformation vectors." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702484.

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Leriche, Françoise. "Recherche d'outils, génétiques utilisables chez la bactérie psycrotrophe MFO, étude de leur comportement aux différentes températures de croissance de la souche et construction d'une fusion traductionnelle par génétique réverse." Rouen, 1993. http://www.theses.fr/1993ROUES040.

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Recherche d'outils génétiques utilisables chez la bactérie pseudomonas fluorescens MFO et mise au point des conditions de leur utilisation aux différentes températures de croissance de la souche. Mise au point d'un vecteur de clonage, de conditions d'intégration de gènes étrangers dans le chromosome bactérien, d'une méthode de cartographie par transfert chromosomique et de transposition par génétique réverse
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張超銘. "Isolation of plasmid-free strain in Lactococcus lactis, and Cloning Epstein-Barr virus (EBV) gp25 gene with L.lactis cloning vector." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/55214993448152301700.

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碩士<br>國立清華大學<br>生物技術研究所<br>90<br>Lactic acid bacteria (LAB) are Gram-positive bacteria and generally regarded as safe (GRAS) organisms. LAB could be used for heterologous protein secretion and fused antigen to form fusion protein. They are good potential candidates as antigen delivery vehicles. We developed an efficient secretion system in the Lactococcus lactis model. Staphylococcal nuclease (Nuc protein) is a small, stable, and biochemically well-characterized enzyme secreted by Gram-positive bacteria. It was used as the reporter protein. Plasmid pNuc10 encoding LEISSTCDA-NucT is
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Sun, Yu-Lin, and 孫玉苓. "Construction of Cloning Vector and Isolation of Plasmid-Free Strain in Radioresistant Bacterium Deinococcus radiodurans." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/16494915504796148876.

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碩士<br>國立清華大學<br>輻射生物研究所<br>81<br>Cloning vector construction and a plasmid-free recipient isolation must be accomplished for developing the gene cloning system in Deinococcus. Two radiation-sensitive mutants, D. radiodurasns S101 and D. radiodurans PF3-3, were isolated from MNNG-mutagenized D. radiodurans strain IR. Both also cured the 28 kb pST7 which is the cryptic plasmid of strain IR and the spontaneous reversion frequencies of them to radiation resistance were 1--5 x 10^-8. This indica
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Lin, Chuen Fu, and 林春福. "Molecular Characterization of Antibiotic Resistance Genes and Replication Regions from Indigenous Plasmids of Lactobacillus reuteri and the Construction of Cloning Vectors for Lactobacillus reuteri." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/08048861640042427942.

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博士<br>國立中興大學<br>獸醫學系<br>87<br>Most of cloning vectors used in Lactobacillus reuteri are adapted from systems already available in other microorganism and generally bear disadvantages of low efficiency and instability. Thus, the aim of this study was to search for some parts (i.e., antibiotic selection markers and replication regions) from indigenous plasmids of this microbe with good properties potentially used in constructing efficient cloning vectors for this bacteria. The final goal of these constructions is to provide tools for the molecular studies, the strain improvement, and the develop
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SU, ZHI-MAO, and 蘇旨茂. "Molecular cloning of a replicon from the plasmid of Nocardia italica and construction of a shuttle vector for E. coli, streptomyces lividans and nocardia italica." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/27043165507261283964.

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Book chapters on the topic "Plasmid cloning vectors"

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Julin, Douglas A. "Plasmid Cloning Vectors." In Molecular Life Sciences. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4614-1531-2_86.

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Julin, Douglas. "Plasmid Cloning Vectors." In Molecular Life Sciences. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6436-5_86-1.

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Gaastra, Wim, and Hans E. N. Bergmans. "Plasmid Derived Cloning Vectors." In Techniques in Molecular Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_6.

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Winstanley, Craig, and Ralph Rapley. "Plasmid-Derived Cloning Vectors." In Springer Protocols Handbooks. Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_16.

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Thompson, Charles J., and Julian E. Davies. "Streptomycete Plasmid Cloning Vectors." In Industrial Aspects of Biochemistry and Genetics. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-1227-7_3.

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Lu, Quinn. "Plasmid Vectors for Gene Cloning and Expression." In Plasmid Biology. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817732.ch27.

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Rabinovich, P. M., M. Ya Haykinson, L. S. Arutyunova, Yu V. Yomantas, and A. I. Stepanov. "The Structure and Source of Plasmid DNA Determine the Cloning Properties of Vectors for Bacillus Subtilis." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_44.

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Denhardt, David T., Dylan R. Edwards, Jacek Kowalski, Craig L. J. Parfett, and Paul Waterhouse. "Specialized Plasmid Vectors for Cloning cDNA." In Vectors. Elsevier, 1988. http://dx.doi.org/10.1016/b978-0-409-90042-2.50018-0.

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Thompson, Russell. "8 Plasmid Cloning Vectors." In Methods in Microbiology. Elsevier, 1988. http://dx.doi.org/10.1016/s0580-9517(08)70075-8.

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Mead, David A., and Byron Kemper. "Chimeric Single-Stranded DNA Phage-Plasmid Cloning Vectors." In Vectors. Elsevier, 1988. http://dx.doi.org/10.1016/b978-0-409-90042-2.50010-6.

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Conference papers on the topic "Plasmid cloning vectors"

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Panfilov, A. V., Yu M. Konstantinov, and M. V. Koulintchenko. "THE OPTIMIZATION OF CLONING OF LONG-SIZED DNA FRAGMENTS IN p-GEM VECTOR, CONTAINING TERMINAL INVERTED REPEATS OF LINEAR MITOCHONDRIAL PLASMIDS FROM ZEA MAYS." In The Second All-Russian Scientific Conference with international participation "Regulation Mechanisms of Eukariotic Cell Organelle Functions". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-318-1-84-86.

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