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Journal articles on the topic 'Plasmid production'

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Published: 4 June 2021

Last updated: 13 February 2022

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1

Trivedi, Ram Narayan, Parvez Akhtar, Jonathan Meade та ін. "High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacBby IntroducingincMutations". Applied and Environmental Microbiology 80, № 23 (2014): 7154–60. http://dx.doi.org/10.1128/aem.02445-14.

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ABSTRACTFor small-copy-number pUC-type plasmids, theinc1andinc2mutations, which deregulate replication, were previously found to increase the plasmid copy number 6- to 7-fold. Because plasmids can exert a growth burden, it was not clear if further amplification of copy number would occur due toincmutations when the starting point for plasmid copy number was orders of magnitude higher. To investigate further the effects of theincmutations and the possible limits of plasmid synthesis, the parent plasmid pNTC8485 was used as a starting point. It lacks an antibiotic resistance gene and has a copy

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Kojic, Milan, Ivana Strahinic, Djordje Fira, Branko Jovcic, and Ljubisa Topisirovic. "Plasmid content and bacteriocin production by five strains ofLactococcus lactisisolated from semi-hard homemade cheese." Canadian Journal of Microbiology 52, no. 11 (2006): 1110–20. http://dx.doi.org/10.1139/w06-072.

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In this study, the plasmid content and bacteriocin production of natural isolates of lactococci were investigated. Five bacteriocin producing lactococcal strains (Lactococcus lactis subsp. lactis BGMN1-2, BGMN1-3, BGMN1-5, BGMN1-6, and BGMN2-7) were isolated as nonstarter microflora of semi-hard homemade cheese and characterized. All isolates contained a number of plasmids. It was shown that lcnB structural genes for bacteriocin lactococcin B were located on large plasmids in all isolates. In the strains BGMN1-3 and BGMN1-5 proteinase prtP genes collocated with lcnB. Furthermore, these strains

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Münch, Karin M., Johannes Müller, Sarah Wienecke, et al. "Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity." Applied and Environmental Microbiology 81, no. 17 (2015): 5976–86. http://dx.doi.org/10.1128/aem.00807-15.

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ABSTRACTDuring the past 2 decades,Bacillus megateriumhas been systematically developed for the gram-per-liter scale production of recombinant proteins. The plasmid-based expression systems employed use a xylose-controlled promoter. Protein production analyses at the single-cell level using green fluorescent protein as a model product revealed cell culture heterogeneity characterized by a significant proportion of less productive bacteria. Due to the enormous size ofB. megaterium, such bistable behavior seen in subpopulations was readily analyzed by time lapse microscopy and flow cytometry. Cel

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Fong, Ryan, Zhihao Hu, C. Richard Hutchinson, Jianqiang Huang, Stanley Cohen, and Camilla Kao. "Characterization of a Large, Stable, High-Copy-Number Streptomyces Plasmid That Requires Stability and Transfer Functions for Heterologous Polyketide Overproduction." Applied and Environmental Microbiology 73, no. 4 (2006): 1296–307. http://dx.doi.org/10.1128/aem.01888-06.

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ABSTRACT A major limitation to improving small-molecule pharmaceutical production in streptomycetes is the inability of high-copy-number plasmids to tolerate large biosynthetic gene cluster inserts. A recent finding has overcome this barrier. In 2003, Hu et al. discovered a stable, high-copy-number, 81-kb plasmid that significantly elevated production of the polyketide precursor to the antibiotic erythromycin in a heterologous Streptomyces host (J. Ind. Microbiol. Biotechnol. 30:516-522, 2003). Here, we have identified mechanisms by which this SCP2*-derived plasmid achieves increased levels of

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Fridjonsson, Olafur, та Ralf Mattes. "Production of Recombinant α-Galactosidases inThermus thermophilus". Applied and Environmental Microbiology 67, № 9 (2001): 4192–98. http://dx.doi.org/10.1128/aem.67.9.4192-4198.2001.

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ABSTRACT A Thermus thermophilus selector strain for production of thermostable and thermoactive α-galactosidase was constructed. For this purpose, the native α-galactosidase gene (agaT) ofT. thermophilus TH125 was inactivated to prevent background activity. In our first attempt, insertional mutagenesis ofagaT by using a cassette carrying a kanamycin resistance gene led to bacterial inability to utilize melibiose (α-galactoside) and galactose as sole carbohydrate sources due to a polar effect of the insertional inactivation. A Gal+ phenotype was assumed to be essential for growth on melibiose.

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Chakrabarty, P. K., Sheo Raj, M. K. Meshram, A. Mahadevan, and D. W. Gabriel. "Plasmid-borne determinants of pigmentation, exopolysaccharide production, and virulence in Xanthomonas campestris pv. malvacearum." Canadian Journal of Microbiology 41, no. 8 (1995): 740–45. http://dx.doi.org/10.1139/m95-101.

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Three plasmids of 55.0, 31.2, and 7.4 kb, designated as pXCM18-I, pXCM18-II, and pXCM18-III, respectively, were identified and isolated from strain HR13B/2 of Xanthomonas campestris pv. malvacearum. The bacterium was cured of all three plasmids in high frequency (3.1%) with heat (42 °C). The cured strains were nonmucoid and light yellow and were greatly impaired in exopolysaccharide production and virulence. Transformation of a cured strain using a mixture of purified plasmid DNA from HR13B/2 resulted in colonies carrying all six possible combinations of the three plasmids. Of the transformant

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7

McMillan, Elizabeth A., Jamie L. Wasilenko, Kaitlin A. Tagg, et al. "Carriage and Gene Content Variability of the pESI-Like Plasmid Associated with Salmonella Infantis Recently Established in United States Poultry Production." Genes 11, no. 12 (2020): 1516. http://dx.doi.org/10.3390/genes11121516.

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Salmonella Infantis carrying extended spectrum β-lactamase blaCTX-M-65 on a pESI-like megaplasmid has recently emerged in United States poultry. In order to determine the carriage rate and gene content variability of this plasmid in U.S. Salmonella Infantis, whole genome sequences of Salmonella isolates from humans and animals in the U.S. and internationally containing the pESI-like plasmid were analyzed. The U.S. Department of Agriculture Food Safety and Inspection Service (FSIS) identified 654 product sampling isolates containing pESI-like plasmids through hazard analysis and critical contro

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8

Ishibashi, Y. "Genetic Studies into Musty Odor Production by Actinomycetes." Water Science and Technology 25, no. 2 (1992): 171–76. http://dx.doi.org/10.2166/wst.1992.0049.

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Musty taste and odor is a serious problem for drinking water. Much data regarding its occurrence are being accumulated. However, we may be able to solve this problem by inquiring into the nature of secondary metabolism through the mevalonic acid pathway in actinomycetes and/or cyanobacteria that yield musty smelling compounds. Therefore, genetic analysis is applied to look for new solutions. By investigating Streptomyces sp. with the protocol to recover plasmid DNA, it was difficult to get the infinitesimally small covalently closed circular (ccc) plasmid. On the other hand, Streptomyces strai

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9

Hausjell, Johanna, Regina Kutscha, Jeannine D. Gesson, Daniela Reinisch, and Oliver Spadiut. "The Effects of Lactose Induction on a Plasmid-Free E. coli T7 Expression System." Bioengineering 7, no. 1 (2020): 8. http://dx.doi.org/10.3390/bioengineering7010008.

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Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host Escherichia coli presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid loss over prolonged cultivations. To improve production, efforts are made to establish plasmid-free expression, ensuring more stable process conditions. Another strategy to stabilize production processes is lactose induction, leading to increased soluble product formation and cell fitness, as shown

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10

Carnes,, Aaron E. "High-Yield Plasmid DNA Production." Genetic Engineering & Biotechnology News 32, no. 8 (2012): 42–43. http://dx.doi.org/10.1089/gen.32.8.18.

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11

Fernández, Antonio, Nikki Horn, Michael J. Gasson, Helen M. Dodd, and Juan M. Rodríguez. "High-level coproduction of the bacteriocins nisin A and lactococcin A by Lactococcus lactis." Journal of Dairy Research 71, no. 2 (2004): 216–21. http://dx.doi.org/10.1017/s0022029904000123.

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In this study, a two-plasmid system for enhanced and consistent biosynthesis of the model lactococcal bacteriocin lactococcin A in non-producing Lactococcus lactis hosts was developed. The system comprised a plasmid carrying the genes lcnA and lciA under the control of the nisin-inducible nisA promoter, and a second plasmid harbouring the lcnC and lcnD genes. The introduction of both plasmids into two strains containing the nisRK genes required for nisin-controlled expression, Lc. lactis FI5876 (a nisin A-producer strain) and FI7847, resulted in production of extracellular lactococcin A at a h

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12

Kojic, Milan, Ivana Strahinic, and Ljubisa Topisirovic. "Proteinase PI and lactococcin A genes are located on the largest plasmid inLactococcus lactissubsp.lactisbv. diacetylactis S50." Canadian Journal of Microbiology 51, no. 4 (2005): 305–14. http://dx.doi.org/10.1139/w05-009.

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Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 µg·mL–1) and sublethal temperature (40 °C) resulted in a very low yield (0.17%) of Prt–, Bac–, Bacsderivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combinat

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ISOM, LOWELL L., ALI H. AHMED, and SCOTT E. MARTIN. "Influence of Plasmids on Catalase and Superoxide Dismutase Activities in Listeria monocytogenes." Journal of Food Protection 58, no. 9 (1995): 955–59. http://dx.doi.org/10.4315/0362-028x-58.9.955.

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Two of nine strains of Listeria monocytogenes examined were found to contain plasmid DNA. Strain 19112 contained a 31.1 kb plasmid and strain 7644 contained a 49.4 kb plasmid. Each of the strains was cured of its plasmid by heat treatment at 46°C. Both the wild-type and plasmid-negative forms of each strain were screened for cadmium resistance. The 31.1 kb plasmid of L. monocytogenes 19112 was shown to confer resistance to cadmium. Listeria monocytogenes 7644 did not show resistance to cadmium. The plasmids for both strains were isolated and purified by CsCl density-gradient centrifugation. Ea

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Rasko, David A., M. J. Rosovitz, Ole Andreas Økstad, et al. "Complete Sequence Analysis of Novel Plasmids from Emetic and Periodontal Bacillus cereus Isolates Reveals a Common Evolutionary History among the B. cereus-Group Plasmids, Including Bacillus anthracis pXO1." Journal of Bacteriology 189, no. 1 (2006): 52–64. http://dx.doi.org/10.1128/jb.01313-06.

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ABSTRACTThe plasmids of the members of theBacillus cereussensu lato group of organisms are essential in defining the phenotypic traits associated with pathogenesis and ecology. For example,Bacillus anthraciscontains two plasmids, pXO1 and pXO2, encoding toxin production and encapsulation, respectively, that define this species pathogenic potential, whereas the presence of a Bt toxin-encoding plasmid definesBacillus thuringiensisisolates. In this study the plasmids fromB. cereusisolates that produce emetic toxin or are linked to periodontal disease were sequenced and analyzed. Two periodontal i

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Ito, Yoshiyuki, Yasushi Kawai, Kensuke Arakawa, Yoshiko Honme, Takashi Sasaki, and Tadao Saito. "Conjugative Plasmid from Lactobacillus gasseri LA39 That Carries Genes for Production of and Immunity to the Circular Bacteriocin Gassericin A." Applied and Environmental Microbiology 75, no. 19 (2009): 6340–51. http://dx.doi.org/10.1128/aem.00195-09.

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ABSTRACT Gassericin A is a circular bacteriocin produced by Lactobacillus gasseri strain LA39. We found a 33,333-bp plasmid, designated pLgLA39, in this strain. pLgLA39 contained 44 open reading frames, including seven genes related to gassericin A production/immunity (gaa), as well as genes for replication, plasmid maintenance, and conjugative transfer. pLgLA39 was transferred from LA39 to the type strain of L. gasseri (JCM 1131) by filter mating. The transconjugant exhibited >30-fold-higher more resistance to gassericin A and produced antibacterial activity. Lactobacillus reuteri LA6, the

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Prather, Kristala Jones, Sangeetha Sagar, Jason Murphy, and Michel Chartrain. "Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification." Enzyme and Microbial Technology 33, no. 7 (2003): 865–83. http://dx.doi.org/10.1016/s0141-0229(03)00205-9.

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Shubin, F. N., A. V. Rakov, N. A. Kuznetsova, T. V. Yakubich, and I. P. Snetkova. "FORMATION OF POPULATION MORBIDITY WITH SALMONELLOSIS CAUSED BY SALMONELLA ENTERITIDIS IN REGIONS WITH INCOMPLETE SUPPLY OF LOCAL POULTRY PRODUCTS." Journal of microbiology epidemiology immunobiology, no. 1 (February 28, 2017): 61–67. http://dx.doi.org/10.36233/0372-9311-2017-1-61-67.

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Aim. Study plasmid characteristics of S. enteritidis strains in patients and features of epidemiology of the infection in regions with incomplete supply of population with local poultry production. Materials and methods. Plasmid analysis of microbe strains isolated from 382 patients and 8 samples of products was carried out, and significance of plasmid types in population morbidity was evaluated. Identification of salmonella was carried out by conventional methods, plasmid specter - by Kado C.I. and Liu S.T. (1981) method. Results. 98.4% of strains contained virulence plasmid p38, and 80.1% of

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18

Soares, Alexandra, Luciana C. Gomes, Gabriel A. Monteiro, and Filipe J. Mergulhão. "The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression." Applied Sciences 11, no. 18 (2021): 8667. http://dx.doi.org/10.3390/app11188667.

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In the present study, the effects of different nutrient media on the development of Escherichia coli biofilms and the production of a heterologous protein were examined. E. coli JM109(DE3) cells transformed with pFM23 plasmid carrying the gene for enhanced green fluorescent protein (eGFP) expression were used. Cells were grown in two different culture media, Lysogenic Broth (LB) and M9ZB, in a flow cell system for 10 days. Epifluorescence microscopy, fluorimetry, and a high-performance liquid chromatography (HPLC) method based on hydrophobic interaction chromatography (HIC) were used to assess

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Santos, Saritza, Maité Ramírez, Eric Miranda, et al. "Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases." Journal of Immunology Research 2019 (May 22, 2019): 1–15. http://dx.doi.org/10.1155/2019/3409371.

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Immunogenicity of DNA vaccines can be efficiently improved by adding adjuvants into their formulations. In this regard, the application of nano- and microparticles as vaccines adjuvants, or delivery systems, provides a powerful tool in designing modern vaccines. In the present study, we examined the role of “Supramolecular Hacky Sacks” (SHS) particles, made via the hierarchical self-assembly of a guanosine derivative, as a novel immunomodulator for DNA plasmid preparations. These plasmids code for the proteins HIV-1 Gag (pGag), the wild-type vaccinia virus Western Reserve A27 (pA27L), or a cod

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Daniel, Ebakota, Osarueme Osazee, Frances Olisaka, Jocelyn Aibangbee, Panmwa GALAU, and Joseph Osazee. "Antibiotic susceptibility, plasmid isolation and curing of some foodborne pathogens." International Journal of Biological Research 4, no. 2 (2016): 321. http://dx.doi.org/10.14419/ijbr.v4i2.6779.

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The indiscriminate use of antibiotics by individuals as well as in food production has been tagged one of the major reasons for the spread of antibiotic resistance in pathogens. Thus, there is a concern that foodborne bacteria may act as reservoirs of antibiotic resistance genes when distributed in large doses with food. This study aimed at determining the antibiotic susceptibility, plasmid isolation and curing of foodborne bacteria isolated from ready to eat (RTE) foods and salads in eating centers at the Benson Idahosa University, Benin City. Isolates were Enterobacter aerogenes, Escherichia

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Bihannic, Morgan, Marisa Haenni, Eric Oswald, and Jean-Yves Madec. "Divergent Evolution of the repFII Replicon of IncF Plasmids Carrying Cytotoxic Necrotizing Factorcnf2, Cytolethal Distending ToxincdtIII, andf17AeFimbrial Variant Genes in Type 2 Necrotoxigenic Escherichia coli Isolates from Calves." Applied and Environmental Microbiology 82, no. 2 (2015): 510–17. http://dx.doi.org/10.1128/aem.02641-15.

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ABSTRACTAmong the pathovars ofEscherichia coliin cattle, necrotoxigenicE. coli(NTEC) is defined by the production of cytotoxic necrotizing factors (CNFs). In particular, type 2 NTEC (NTEC2) strains are frequent in diarrheic and septicemic calves and usually coproduce CNF type 2 (CNF2), cytolethal distending toxin type III (CDTIII), and fimbrial adhesins of the F17 family, whose genetic determinants have frequently been reported on the same Vir-like plasmid. In this study, we investigated the genetic environment of thecnf2,f17Ae, andcdtIIIgenes in a collection of fecalE. coliisolates recovered

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McClure, Nicholas C., Ali-Reza Ahmadi, and Bruce G. Clare. "Construction of a Range of Derivatives of the Biological Control Strain Agrobacterium rhizogenes K84: a Study of Factors Involved in Biological Control of Crown Gall Disease." Applied and Environmental Microbiology 64, no. 10 (1998): 3977–82. http://dx.doi.org/10.1128/aem.64.10.3977-3982.1998.

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ABSTRACT The biological control strain Agrobacterium rhizogenesK84 is an effective agent in the control of Agrobacteriumpathogens, the causative agents of crown gall disease. A number of factors are thought to play a role in the control process, including production of the specific agrocins 84 and 434, which differ in the spectra of pathogenic strains that they inhibit in vitro. A range of derivatives of strain K84 has been developed with every combination of the three resident plasmids, pAgK84, pAgK434, and pAtK84b, including a plasmid-free strain. These derivatives produced either both, one,

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Parente, Ana Flávia, Ildinete Silva-Pereira, José Ivo Baldani, Victor Hugo da Silva Tibúrcio, Sônia Nair Báo, and Marlene T. De-Souza. "Construction ofBacillus thuringiensiswild-type S76 and Cry–derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria–plant interactions." Canadian Journal of Microbiology 54, no. 9 (2008): 786–90. http://dx.doi.org/10.1139/w08-061.

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Collectively, the species Bacillus thuringiensis , Bacillus cereus , and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance. Although the genetic correlation and pathogenic characteristics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood. Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was

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May, Thithiwat, and Satoshi Okabe. "Escherichia coli Harboring a Natural IncF Conjugative F Plasmid Develops Complex Mature Biofilms by Stimulating Synthesis of Colanic Acid and Curli." Journal of Bacteriology 190, no. 22 (2008): 7479–90. http://dx.doi.org/10.1128/jb.00823-08.

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ABSTRACT It has been shown that Escherichia coli harboring the derepressed IncFI and IncFII conjugative F plasmids form complex mature biofilms by using their F-pilus connections, whereas a plasmid-free strain forms only patchy biofilms. Therefore, in this study we investigated the contribution of a natural IncF conjugative F plasmid to the formation of E. coli biofilms. Unlike the presence of a derepressed F plasmid, the presence of a natural IncF F plasmid promoted biofilm formation by generating the cell-to-cell mating F pili between pairs of F+ cells (approximately two to four pili per cel

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Mingyar, Erik, Lucas Mühling, Andreas Kulik, et al. "A Regulator Based “Semi-Targeted” Approach to Activate Silent Biosynthetic Gene Clusters." International Journal of Molecular Sciences 22, no. 14 (2021): 7567. http://dx.doi.org/10.3390/ijms22147567.

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By culturing microorganisms under standard laboratory conditions, most biosynthetic gene clusters (BGCs) are not expressed, and thus, the products are not produced. To explore this biosynthetic potential, we developed a novel “semi-targeted” approach focusing on activating “silent” BGCs by concurrently introducing a group of regulator genes into streptomycetes of the Tübingen strain collection. We constructed integrative plasmids containing two classes of regulatory genes under the control of the constitutive promoter ermE*p (cluster situated regulators (CSR) and Streptomyces antibiotic regula

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Paula, Marcia O., Elerson GaettiI-Jardim Jr., and Mario J. Avilla-Campos. "Plasmid profile in oral Fusobacterium nucleatum from humans and Cebus apella monkeys." Revista do Instituto de Medicina Tropical de São Paulo 45, no. 1 (2003): 05–09. http://dx.doi.org/10.1590/s0036-46652003000100002.

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Fusobacterium nucleatum is a strict anaerobe and is indigenous of the human oral cavity. This organism is commonly recovered from different monomicrobial and mixed infections in humans and animals. In this study, the plasmid profile, the plasmid stability and the penicillin-resistance association in oral F. nucleatum isolated from periodontal patients, healthy subjects and Cebus apella monkeys were evaluated. Forty-five F. nucleatum strains from patients, 38 from healthy subjects and seven from C. apella were identified and analyzed. Plasmid extraction was performed in all the isolated strains

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27

Kalousek, S., та W. Lubitz. "High-level poly(β-hydroxybutyrate) production in recombinantEscherichia coliin sugar-free, complex medium". Canadian Journal of Microbiology 41, № 13 (1995): 216–21. http://dx.doi.org/10.1139/m95-190.

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The poly(β-hydroxybutyrate) (PHB) biosynthetic genes of Alcaligenes eutrophus that are organized in a single operon (phbCAB) have been cloned in Escherichia coli, where the expression of the genes in the wild-type phb operon from plasmid p4A leads to the formation of 10 or 50–80% PHB/cell dry mass when the cells are grown in Luria–Bertani medium alone or supplemented with 1% glucose (w/v), respectively. To further stimulate PHB formation independent of additional carbon source in Luria–Bertani medium, molecular methods have been applied to provide efficient E. coli transcription and translatio

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Hansen, Katrine Hartung, Valeria Bortolaia, Christine Ahl Nielsen та ін. "Host-Specific Patterns of Genetic Diversity among IncI1-Iγ and IncK Plasmids Encoding CMY-2 β-Lactamase in Escherichia coli Isolates from Humans, Poultry Meat, Poultry, and Dogs in Denmark". Applied and Environmental Microbiology 82, № 15 (2016): 4705–14. http://dx.doi.org/10.1128/aem.00495-16.

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ABSTRACTCMY-2 is the most common plasmid-mediated AmpC β-lactamase inEscherichia coliisolates of human and animal origin. The aim of this study was to elucidate the epidemiology of CMY-2-producingE. coliin Denmark. Strain and plasmid relatedness was studied in 93 CMY-2-producing clinical and commensalE. coliisolates collected from 2006 to 2012 from humans, retail poultry meat, broilers, and dogs. Multilocus sequence typing (MLST), antimicrobial susceptibility testing, and conjugation were performed in conjunction with plasmid replicon typing, plasmid multilocus sequence typing (pMLST), restric

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Zhao, Jing-yi, Li Zhong, Mei-juan Shen, et al. "Discovery of the Autonomously Replicating Plasmid pMF1 from Myxococcus fulvus and Development of a Gene Cloning System in Myxococcus xanthus." Applied and Environmental Microbiology 74, no. 7 (2008): 1980–87. http://dx.doi.org/10.1128/aem.02143-07.

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ABSTRACT Myxobacteria are very important due to their unique characteristics, such as multicellular social behavior and the production of diverse and novel bioactive secondary metabolites. However, the lack of autonomously replicating plasmids has hindered genetic manipulation of myxobacteria for decades. To determine whether indigenous plasmids are present, we screened about 150 myxobacterial strains, and a circular plasmid designated pMF1 was isolated from Myxococcus fulvus 124B02. Sequence analysis showed that this plasmid was 18,634 bp long and had a G+C content of 68.7%. Twenty-three open

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30

Steinmetz, Eric. "Low Endotoxin Plasmid Production from E. coli." Genetic Engineering & Biotechnology News 34, no. 4 (2014): 16–17. http://dx.doi.org/10.1089/gen.34.04.10.

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San Millán, J. L., R. Kolter, and F. Moreno. "Plasmid genes required for microcin B17 production." Journal of Bacteriology 163, no. 3 (1985): 1016–20. http://dx.doi.org/10.1128/jb.163.3.1016-1020.1985.

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32

Kanatani, Kazuo, and Masao Oshimura. "Plasmid-associated Bacteriocin Production by aLactobacillus plantarumStrain." Bioscience, Biotechnology, and Biochemistry 58, no. 11 (1994): 2084–86. http://dx.doi.org/10.1271/bbb.58.2084.

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Vescovo, Marisa, G. L. Scolari, and V. Bottazzi. "Plasmid-encoded ropiness production inLactobacillus casei SSP.casei." Biotechnology Letters 11, no. 10 (1989): 709–12. http://dx.doi.org/10.1007/bf01044102.

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Bergman, L. W., M. C. Stranathan, and L. H. Preis. "Structure of the transcriptionally repressed phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 1 (1986): 38–46. http://dx.doi.org/10.1128/mcb.6.1.38.

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We developed a high-copy-number plasmid system containing the entire structural and regulatory sequences of the phosphate-repressible acid phosphatase (PHO5) gene and the TRP1/ARS1 replicator sequences of the yeast Saccharomyces cerevisiae to investigate the mechanism of repression-derepression of transcription. The resulting plasmid was used to transform either wild-type cells or a number of strains which contain mutations in various trans-acting regulatory loci for the production of acid phosphatase. Results of analysis of mRNA levels isolated from the transformed strains grown under repress

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Bergman, L. W., M. C. Stranathan, and L. H. Preis. "Structure of the transcriptionally repressed phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 1 (1986): 38–46. http://dx.doi.org/10.1128/mcb.6.1.38-46.1986.

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We developed a high-copy-number plasmid system containing the entire structural and regulatory sequences of the phosphate-repressible acid phosphatase (PHO5) gene and the TRP1/ARS1 replicator sequences of the yeast Saccharomyces cerevisiae to investigate the mechanism of repression-derepression of transcription. The resulting plasmid was used to transform either wild-type cells or a number of strains which contain mutations in various trans-acting regulatory loci for the production of acid phosphatase. Results of analysis of mRNA levels isolated from the transformed strains grown under repress

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Wang, Dongguo, Wei Hou, Jiayu Chen, et al. "Characterization of the bla KPC-2 and bla KPC-3 genes and the novel bla KPC-15 gene in Klebsiella pneumoniae." Journal of Medical Microbiology 63, no. 7 (2014): 981–87. http://dx.doi.org/10.1099/jmm.0.073841-0.

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Three Klebsiella pneumoniae isolates exhibiting high-level resistance to carbapenem were analysed by PCR, PFGE, gene mapping, plasmid conjugation and Southern blot hybridization using a bla KPC probe. In addition to the frequently reported bla KPC-2 and bla KPC-3 genes, a novel bla KPC-15 gene was identified in one of the isolates. The results of plasmid analysis and Southern blot hybridization revealed that the three bla KPC genes were located on transferable plasmids exhibiting three different patterns. The patterns A, B and C were observed in the genetic makeup of each individual plasmid, a

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Urthaler, Jochen, Wolfgang Buchinger, and Roman Necina. "Improved downstream process for the production of plasmid DNA for gene therapy." Acta Biochimica Polonica 52, no. 3 (2005): 703–11. http://dx.doi.org/10.18388/abp.2005_3434.

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Gene therapy and genetic vaccines promise to revolutionize the treatment of inherited and acquired diseases. Since viral vectors are generally associated with numerous disadvantages when applied to humans, the administration of naked DNA, or DNA packed into lipo- or polyplexes emerge as viable alternatives. To satisfy the increasing demand for pharmaceutical grade plasmids we developed a novel economic downstream process which overcomes the bottlenecks of common lab-scale techniques and meets all regulatory requirements. After cell lysis by an in-house developed gentle, automated continuous sy

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Zingali, Tiziana, Toni A. Chapman, John Webster, Piklu Roy Chowdhury, and Steven P. Djordjevic. "Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in Escherichia coli ST744 in Australia." Microorganisms 8, no. 6 (2020): 896. http://dx.doi.org/10.3390/microorganisms8060896.

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Antibiotic resistance genes (ARGs) including those from the blaCTX-M family and mcr-1 that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal E. coli from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sul

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Silva, Filomena, João A. Queiroz, and Fernanda C. Domingues. "Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli." Biotechnology Advances 30, no. 3 (2012): 691–708. http://dx.doi.org/10.1016/j.biotechadv.2011.12.005.

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Zhao, Shuang-Xia, Shanli Tsui, Anthony Cheung, Raymond S. Douglas, Terry J. Smith, and J. Paul Banga. "Orbital fibrosis in a mouse model of Graves' disease induced by genetic immunization of thyrotropin receptor cDNA." Journal of Endocrinology 210, no. 3 (2011): 369–77. http://dx.doi.org/10.1530/joe-11-0162.

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The TSH receptor (TSHR) is the critical target for antibody production in Graves' disease (GD). Insulin-like growth factor 1 receptor (IGF1R) has been proposed as a second autoantigen in complications of GD such as orbitopathy. We attempted to induce orbital tissue remodeling in mice undergoing immunizations with plasmids encoding TSHR and IGF1R delivered byin vivoskeletal muscle electroporation, a procedure known to give a sustained, long-term antibody response. FemaleBALB/cmice were challenged with TSHR A-subunit or IGF1Rα subunit plasmid by injection and electroporation. Mice challenged wit

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Aqua, Mfon S., David Svinarich, Anju Dhar, and Sunil Palchaudhuri. "The stability of O-antigen plasmid is determined by a chromosomal region of Shigella dysenteriae." Canadian Journal of Microbiology 34, no. 1 (1988): 58–62. http://dx.doi.org/10.1139/m88-010.

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It is well established that plasmids are involved in the expression of lipopolysaccharide in certain species of Shigella. In Shigella sonnei, both the biosynthesis of oligosaccharide side chains (O antigen), and cell invasiveness are controlled exclusively by a 120 megadalton (MDa) plasmid. In Shigella dysenteriae 1, a 10 kilobase (kb) plasmid is required for O-antigen production. Shigella dysenteriae 1 strains devoid of this plasmid lose the ability to synthesize O antigen. Interestingly, this 10-kb plasmid is not stably maintained in Escherichia coli K-12 strains, where it is lost spontaneou

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Yamaguchi, Takayuki, Tetsuya Hayashi, Hideto Takami, et al. "Complete Nucleotide Sequence of aStaphylococcus aureus Exfoliative Toxin B Plasmid and Identification of a Novel ADP-Ribosyltransferase, EDIN-C." Infection and Immunity 69, no. 12 (2001): 7760–71. http://dx.doi.org/10.1128/iai.69.12.7760-7771.2001.

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ABSTRACT The complete nucleotide sequence of pETB, a 38.2-kbStaphylococcus aureus plasmid encoding the exfoliative toxin B (ETB), was determined. A total of 50 open reading frames were identified on the plasmid genome and, among these, 32 showed sequence similarity to known proteins. pETB contains three copies of IS257, which divide the pETB genome into three regions: (i) a cadmium resistance operon-containing region, (ii) a lantibiotic production gene-containing region, and (iii) the remaining part where genes for plasmid replication and/or maintenance are dispersed. In the third region, gene

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Deneke, Jan, and George Chaconas. "Purification and Properties of the Plasmid Maintenance Proteins from the Borrelia burgdorferi Linear Plasmid lp17." Journal of Bacteriology 190, no. 11 (2008): 3992–4000. http://dx.doi.org/10.1128/jb.00057-08.

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ABSTRACT The Lyme disease spirochete Borrelia burgdorferi carries more plasmids than any other bacterium, many of which are linear with covalently closed hairpin ends. These plasmids have also been referred to as mini-chromosomes and essential genetic elements and are integral components of its segmented genome. We have investigated two plasmid maintenance proteins, BBD14 (the replication initiator) and BBD21 (a presumptive ParA orthologue), encoded by the linear plasmid lp17; these proteins are representatives of paralogous families 62 and 32, respectively. We have purified recombinant 6-his-

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Schmidt-Eisenlohr, Heike, and Christian Baron. "The Competitiveness of Pseudomonas chlororaphis Carrying pJP4 Is Reduced in the Arabidopsis thaliana Rhizosphere." Applied and Environmental Microbiology 69, no. 3 (2003): 1827–31. http://dx.doi.org/10.1128/aem.69.3.1827-1831.2003.

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ABSTRACT The effect of the large catabolic IncP plasmid pJP4 on the competitiveness of Pseudomonas chlororaphis SPR044 and on its derivatives SPR244 (GacS deficient), SPR344 (phenazine-1-carboxamide overproducer), and SPR644 (phenazine-1-carboxamide deficient) in the Arabidopsis thaliana rhizosphere was assessed. Solitary rhizosphere colonization by the wild type, SPR244, and SPR644 was not affected by the plasmid. The size of the population of SPR344 carrying pJP4, however, was significantly reduced compared to the size of the population of the plasmid-free derivative. The abiotic stress caus

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Draths, K. M., and John W. Frost. "Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production." Journal of the American Chemical Society 112, no. 4 (1990): 1657–59. http://dx.doi.org/10.1021/ja00160a071.

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Agodi, A., C. Jones, E. J. Threlfall, M. D'Angelo, and M. Marranzano. "Molecular characterization of trimethoprim resistance inShigella sonneiin Sicily." Epidemiology and Infection 105, no. 1 (1990): 29–40. http://dx.doi.org/10.1017/s0950268800047610.

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SUMMARYDuring the 3-year period 1985–7, all strains ofShigella sonneiisolated in Catania, Sicily, showed a high level of resistance to trimethoprim (Tp) which was invariably associated with resistance to other antibiotics.Plasmid analysis showed 18 different electropherotypes: 35 of 37 strains harboured a plasmid of 70 Megadaltons (MDa), and 29 of 37 strains a plasmid of 130 MDa.Restriction endonuclease fingerprinting of purified 70 MDa plasmid DNA from different strains demonstrated that these plasmids were similar but not identical.In some strains with transferable Tp resistance, DNA hybridi

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Li, Huaiyu, Saumya Bhaduri, and Wayne E. Magee. "Maximizing Plasmid Stability and Production of Released Proteins in Yersinia enterocolitica." Applied and Environmental Microbiology 64, no. 5 (1998): 1812–15. http://dx.doi.org/10.1128/aem.64.5.1812-1815.1998.

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ABSTRACT Virulent serotypes of Yersinia enterocolitica carry a plasmid (pYV) encoding a family of proteins that are released into the medium and whose expression is temperature and calcium regulated. The plasmid is easily lost from cells during their growth in the laboratory. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a monoclonal antibody (3.2C) that is specific for a 25-kDa released protein to show that 32Ã¯Â¿Â½C is the lowest temperature at which plasmid-encoded proteins are expressed in quantity. The highest calcium concentration allowi

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Lee, Sang Yup, and Young Lee. "Metabolic Engineering of Escherichia coli for Production of Enantiomerically Pure (R)-(−)-Hydroxycarboxylic Acids." Applied and Environmental Microbiology 69, no. 6 (2003): 3421–26. http://dx.doi.org/10.1128/aem.69.6.3421-3426.2003.

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ABSTRACT A heterologous metabolism of polyhydroxyalkanoate (PHA) biosynthesis and degradation was established in Escherichia coli by introducing the Ralstonia eutropha PHA biosynthesis operon along with the R. eutropha intracellular PHA depolymerase gene. By with this metabolically engineered E. coli, enantiomerically pure (R)-3-hydroxybutyric acid (R3HB) could be efficiently produced from glucose. By employing a two-plasmid system, developed as the PHA biosynthesis operon on a medium-copy-number plasmid and the PHA depolymerase gene on a high-copy-number plasmid, R3HB could be produced with a

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Inglis, R. Fredrik, Bihter Bayramoglu, Osnat Gillor, and Martin Ackermann. "The role of bacteriocins as selfish genetic elements." Biology Letters 9, no. 3 (2013): 20121173. http://dx.doi.org/10.1098/rsbl.2012.1173.

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Bacteria produce a wide arsenal of toxic compounds in order to kill competing species. Bacteriocins, protein-based toxins produced by nearly all bacteria, have generally been considered a ubiquitous anti-competitor strategy, used to kill competing bacterial strains. Some of these bacteriocins are encoded on plasmids, which also code for closely linked immunity compounds (thereby rendering toxin producing cells immune to their own toxin). However, the production of bacteriocins can also be interpreted as a means to promote plasmid stability by preferentially selecting for cells carrying the pla

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de la Cruz, Mitzi, Elisa A. Ramírez, Juan-Carlos Sigala, José Utrilla, and Alvaro R. Lara. "Plasmid DNA Production in Proteome-Reduced Escherichia coli." Microorganisms 8, no. 9 (2020): 1444. http://dx.doi.org/10.3390/microorganisms8091444.

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The design of optimal cell factories requires engineering resource allocation for maximizing product synthesis. A recently developed method to maximize the saving in cell resources released 0.5% of the proteome of Escherichia coli by deleting only three transcription factors. We assessed the capacity for plasmid DNA (pDNA) production in the proteome-reduced strain in a mineral medium, lysogeny, and terrific broths. In all three cases, the pDNA yield from biomass was between 33 and 53% higher in the proteome-reduced than in its wild type strain. When cultured in fed-batch mode in shake-flask, t

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