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Journal articles on the topic 'Plasmid profiles'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Giles, Janice S., Harry Hariharan, and Susan B. Heaney. "The plasmid profiles of fish pathogenic isolates of Aeromonas salmomcida, Vibrio anguillarum, and Vibrio ordalii from the Atlantic and Pacific coasts of Canada." Canadian Journal of Microbiology 41, no. 3 (1995): 209–16. http://dx.doi.org/10.1139/m95-029.

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The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. Bacterial isolates were from disease outbreaks in fish on the Atlantic and Pacific coasts. Resistant isolates were examined when grown in the presence and absence of antibiotic. Alkaline lysis methods were used for plasmid isolation. Vibrio spp. were predominantly plasmidless, except for a 47-kilobase (kb) plasmid. Atlantic coast isolates of A. salmonicida possessed four or six plasmids, with four smaller pla

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2

McDonald, Timothy J., and George W. Pugh. "Plasmid profiles of Moraxella bovis isolates." American Journal of Veterinary Research 47, no. 4 (1986): 964–66. https://doi.org/10.2460/ajvr.1986.47.04.964.

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SUMMARY Two-hundred isolates of Moraxella bovis were selected at random and examined for the presence of plasmid DNA by a rapid alkaline-detergent lysis method. All isolates contained from 1 to 6 plasmids, with varying agarose-gel electrophoretic migration patterns. Most (80%) isolates carried 2 to 4 plasmids, which ranged in molecular weight from 2.6 to 80 megadaltons. Seemingly, plasmid profiles can be used as a simple, reliable epizootiologic tool to establish a strain identification scheme for M bovis.

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3

Pedersen, K., T. Tiainen, and J. L. Larsen. "Plasmid profiles, restriction fragment length polymorphisms and O-serotypes amongVibrio anguillarumisolates." Epidemiology and Infection 117, no. 3 (1996): 471–78. http://dx.doi.org/10.1017/s0950268800059136.

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SummaryA total of 279Vibrio anguillarumstrains were serotyped and examined for plasmid content. Plasmids were subjected to digestion with restriction enzymes. Most strains belonged to serogroup O1 (39%) and O2 (16%). In total 164 strains (53%) carried plasmids. Of the O1 and O2 isolates, 92% and 30%, respectively, carried one or more plasmids. Restriction fragment length polymorphism (RFLP) analysis of plasmid DNA indicated that plasmids belonged to several groups. Each group seemed to be restricted to a single O-serovar. The largest group was the pJM1-like plasmids among most serovar O1 strai

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4

Gebre-Yohannes, A., and B. S. Drasar. "Plasmid profiles of antibiotic-resistantShigella dysenteriaetypes 2, 3, 4, 6 and 7 isolated in Ethiopia during 1976–85." Epidemiology and Infection 105, no. 1 (1990): 65–72. http://dx.doi.org/10.1017/s0950268800047658.

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SUMMARYPlasmid profile analysis by agarose gel electrophoresis was carried out on 37 drug-resistant strains ofShigella dysenteriaetypes 2, 3, 4, 6 and 7. These strains were collected between 1976 and 1985 in Addis Ababa, Ethiopia.The plasmid profile ofS. dysenteriaetype 2 strains with R-type CSSuT did not show middle-sized plasmids likely to code for CSSuT resistance. All strains contained a large plasmid of about 120 megadaltons (MDa), and a cryptic plasmid of about 2·2 MDa. The plasmid profiles ofS. dysenteriaetype 3 with R-types ACSSuT, SSuT and SSu showed a 4·2 MDa SSu-determinant, which w

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5

Cantin, Mario, Josée Harel, Robert Higgins, and Marcelo Gottschalk. "Antimicrobial Resistance Patterns and Plasmid Profiles of Streptococcus Suis Isolates." Journal of Veterinary Diagnostic Investigation 4, no. 2 (1992): 170–74. http://dx.doi.org/10.1177/104063879200400209.

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Streptococcus suis isolates recovered from diseased animals in Quebec and western Canada and from human cases in Europe were tested for their susceptibility to different antimicrobial agents and screened for their plasmid content. Most isolates from Quebec were clindamycin, erythromycin, and tetracycline resistant; animal isolates from western Canada were notably less resistant to clindamycin and erythromycin, whereas human isolates were considerably more susceptible to most antimicrobials tested.1 More than 60% of isolates had plasmids that ranged from 1.5 to 35 kilobases (kb). Of the 7 plasm

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Boosinger, Timothy R., Willard T. Blevins, James V. Heron, and Janet L. Sunter. "Plasmid profiles of six species of Campylobacter from human beings, swine, and sheep." American Journal of Veterinary Research 51, no. 5 (1990): 718–22. http://dx.doi.org/10.2460/ajvr.1990.51.05.718.

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Summary Twenty-four isolates representing 6 species of Campylobacter were screened for plasmids. A large plasmid with an approximate molecular weight of 38 Mdal was detected in 5 C jejuni isolates originally recovered from diarrheic human beings, in one isolate of C coli recovered from diarrheic pigs, and in 1 isolate of C sputorum ssp mucosalis and 2 isolates of C hyointestinalis recovered from pigs with proliferative enteritis. One isolate of C coli and 1 isolate of C hyointestinalis contained an additional smaller plasmid with an approximate molecular weight of 1.6 Mdal; this plasmid was pa

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7

Shaib, Houssam, Paul Aoun, Ahmad Ghaddar, Hamza Al Labadi, and Youssef Obeid. "Multidrug Resistance and Plasmid Profiles of Escherichia coli Isolated from Lebanese Broiler Farms." International Journal of Microbiology 2023 (June 1, 2023): 1–8. http://dx.doi.org/10.1155/2023/8811675.

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The present study was undertaken to determine the antimicrobial resistance patterns and plasmid fingerprints of commensal Escherichia coli isolated from Lebanese broiler chickens. To that end, a total of 30 E. coli isolates were collected from 15 semi-open broiler farms from North Lebanon and Bekaa Valley. Results showed that all the isolates were resistant to at least nine out of 18 evaluated antimicrobial agents. The best-performing antibiotic families were Carbapenems (Imipenem) and Quinolones (Ciprofloxacin and Norfloxacin) to which only 0.0 and 8.3% of the isolates were resistant, respect

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8

Olsen, J. E., D. J. Brown, D. L. Baggesen, and M. Bisgaard. "Biochemical and molecular characterization ofSalmonella entericaserovarberta, and comparison of methods for typing." Epidemiology and Infection 108, no. 2 (1992): 243–60. http://dx.doi.org/10.1017/s0950268800049724.

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SUMMARYStrains ofSalmonella entericaserovarberta(S. berta) from Denmark and seven other countries have been characterized with the aim of developing a rational typing strategy in connection with outbreak investigations.Biotyping divided the strains into H2S-positive (90%) and H2S-negative (10%) biovars. Six percent of the strains were resistant to one or more antimicrobial agents. Eighty-eight percent of the strains carried plasmids and 52 different plasmid profiles were recognized. Six of the common plasmid sizes in these profiles were shown by restriction enzyme analyses to contain more than

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Rossmanith, Sara Edna Rowe, G. R. Wilt, and Gin Wu. "Characterization and comparison of antimicrobial susceptibilities and outer membrane protein and plasmid DNA profiles of Pasteurella haemolytica and certain other members of the genus Pasteurella." American Journal of Veterinary Research 52, no. 12 (1991): 2016–22. http://dx.doi.org/10.2460/ajvr.1991.52.12.2016.

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SUMMARY The outer membrane protein (omp), plasmid, and antimicrobial resistance profiles of Pasteurella haemolytica serotypes 1 through 12, a bovine isolate of P multocida, a chicken isolate of P multocida, and an unidentified Pasteurella species of bovine origin were examined. Isolates of P haemolytica serotypes belonging to the same biotype possessed similar omp profiles. Biotype A isolates contained 2 prominent omp of 43 kilodaltons (kD) and 29 kD, whereas biotype-T serotypes contained 3 major omp of 43, 36, and 25 kD. The major omp profiles of the 2 P multocida isolates and the unidentifie

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10

Johnson, Timothy J., Yvonne M. Wannemuehler, Sara J. Johnson, et al. "Plasmid Replicon Typing of Commensal and Pathogenic Escherichia coli Isolates." Applied and Environmental Microbiology 73, no. 6 (2007): 1976–83. http://dx.doi.org/10.1128/aem.02171-06.

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ABSTRACT Despite the critical role of plasmids in horizontal gene transfer, few studies have characterized plasmid relatedness among different bacterial populations. Recently, a multiplex PCR replicon typing protocol was developed for classification of plasmids occurring in members of the Enterobacteriaceae. Here, a simplified version of this replicon typing procedure which requires only three multiplex panels to identify 18 plasmid replicons is described. This method was used to screen 1,015 Escherichia coli isolates of avian, human, and poultry meat origin for plasmid replicon types. Additio

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Mayer, L. W. "Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance." Clinical Microbiology Reviews 1, no. 2 (1988): 228–43. http://dx.doi.org/10.1128/cmr.1.2.228.

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Plasmids are circular deoxyribonucleic acid molecules that exist in bacteria, usually independent of the chromosome. The study of plasmids is important to medical microbiology because plasmids can encode genes for antibiotic resistance or virulence factors. Plasmids can also serve as markers of various bacterial strains when a typing system referred to as plasmid profiling, or plasmid fingerprinting is used. In these methods partially purified plasma deoxyribonucleic acid species are separated according to molecular size by agarose gel electrophoresis. In a second procedure, plasmid deoxyribon

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Valentine, Carrie R., Susan H. Yandle, Frederic J. Marsik, Jack R. Ebright, and Mark S. Dawson. "Evaluation of the Variety of Plasmid Profiles inS epidermidisIsolates from Hospital Patients and Staff." Infection Control & Hospital Epidemiology 9, no. 10 (1988): 441–46. http://dx.doi.org/10.1086/645740.

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AbstractPlasmid profiling was used to determine the variability of normal flora isolates ofStaphylococcus epidermidisin order to evaluate the usefulness of plasmid profiling for identifying pathogens. Fifteen hospital staff members and patients repeatedly had cultures taken from the hands and nares, and multiple isolates were examined for plasmid profiles.S epidermidisisolated from the nares of 15 neonates were also examined. The total number of isolates examined for plasmid profiles was 726. Repetition of profiles was common among the different isolates from a single sampling (one swab). The

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Peighambari, Seyed Mostafa, Azam Yazdani, Hanieh Taheri, and Fereshteh Shahcheraghi. "Plasmid Profile and Enterobacterial Repetitive Intergenic Consensus-PCR Characterization of Salmonella Infantis Isolates Recovered From Poultry Sources." Iranian Journal of Veterinary Medicine 17, no. 1 (2023): 27–36. http://dx.doi.org/10.32598/ijvm.17.1.1005073.

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Background: Salmonella is known as one of the most important bacterial agents infecting both humans and animals. Salmonella Infantis has been reported as one of the 15 most prevalent serovars worldwide. Despite its clinical importance, there is little information on the molecular characteristics of S. Infantis in Iran. Objectives: This study was conducted to characterize S. Infantis isolates collected from poultry sources in the last decade. The isolates were typified by plasmid profile and enterobacterial repetitive intergenic consensus (ERIC-PCR). Methods: Forty S. Infantis isolates from pou

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YILDIRMAK, T., A. YAZGAN, and G. OZCENGIZ. "Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey." Epidemiology and Infection 121, no. 2 (1998): 303–7. http://dx.doi.org/10.1017/s0950268898001253.

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A total of 259 clinical isolates of nonrepetitive non-typhi salmonellae (NTS) were examined for antibiotic resistance patterns and plasmid content. The antibiotics used were amoxicillin-clavulanic acid (AMC), ampicillin (AM), aztreonam (ATM), carbenicillin (CB), cefixime (CFM), cefotaxime (CTX), cefoxitin (FOX), ceftazidime (CAZ), ceftriaxone (CRO), chloramphenicol (C), ciprofloxacin (CIP), gentamicin (GM), imipenem (IPM), ofloxacin (OFX), tetracycline (TE), trimethoprim-sulfomethoxazole (SXT). Multi-drug resistant (MDR) strains comprised 19·3% of the total isolates (50/259) and almost all wer

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15

G, SUMITHRA T., V. K. CHATURVEDI, P. K. GUPTA, et al. "Plasmid profiling and comparison of the plasmid profiles with virulence of Indian Salmonella Typhimurium strains of varied origin." Indian Journal of Animal Sciences 84, no. 5 (2014): 498–502. http://dx.doi.org/10.56093/ijans.v84i5.40648.

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Among 2,668 Salmonella enterica serovars Salmonella Typhimurium is the most common serovar and discrimination of isolates within this serovar is necessary for the effective epidemiological surveillance and control. Plasmid profiling is an efficient tool for discrimination of bacterial isolates. Hence, 22 strains of S. Typhimurium isolated from different parts of India were analyzed by plasmid profiling to check its differentiation power and to correlate the results with virulence. Nine different sized bands were detected after plasmid isolation with 90.0 kb band shared by most isolates. At lea

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Shears, P., G. Suliman, and C. A. Hart. "Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan." Epidemiology and Infection 103, no. 3 (1989): 487–96. http://dx.doi.org/10.1017/s0950268800030892.

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SUMMARYThe investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes;PstI,EcoR I,HindIII andAraII. Fifteen different digest profiles were

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Chung, Kuang-Ren, Adrian Leuchtmann, and Christopher L. Schardl. "Inheritance of Mitochondrial DNA and Plasmids in the Ascomycetous Fungus, Epichloë typhina." Genetics 142, no. 1 (1996): 259–65. http://dx.doi.org/10.1093/genetics/142.1.259.

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We analyzed the inheritance of mitochondrial DNA (mtDNA) species in matings of the grass symbiont Epichloë typhina. Eighty progeny were analyzed from a cross in which the maternal (stromal) parent possessed three linear plasmids, designated Callan-a (7.5 kb), Aubonne-a (2.1 kb) and Bergell (2.0 kb), and the paternal parent had one plasmid, Aubonne-b (2.1 kb). Maternal transmission of all plasmids was observed in 76 progeny; two progeny possessed Bergell and Callan-a, but had the maternal Aubonne-a replaced with the related paternal plasmid Aubonne-b; two progeny lacked Callan-a, but had the ot

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BICHLER, LOIS A., KAKAMBI V. NAGARAJA, and BENJAMIN S. POMEROY. "Plasmid Diversity in Salmonella enteritidis of Animal, Poultry, and Human Origin." Journal of Food Protection 57, no. 1 (1994): 4–11. http://dx.doi.org/10.4315/0362-028x-57.1.4.

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One hundred thirty-eight isolates of Salmonella enteritidis from human, animal, and avian species were analyzed for the presence of plasmid DNA. Plasmid DNA from S. enteritidis isolates were extracted by a modification of a high alkaline extraction procedure. Comparisons were made between samples based on the number of plasmids present and their molecular weights. There were seven different profiles seen among the 15 human isolates from the Centers for Disease Control. These seven profiles were recognized with the animal isolates from the National Veterinary Services Laboratory, the chicken is

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Nishida, Hiromi. "Comparative Analyses of Base Compositions, DNA Sizes, and Dinucleotide Frequency Profiles in Archaeal and Bacterial Chromosomes and Plasmids." International Journal of Evolutionary Biology 2012 (March 26, 2012): 1–5. http://dx.doi.org/10.1155/2012/342482.

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In the present paper, I compared guanine-cytosine (GC) contents, DNA sizes, and dinucleotide frequency profiles in 109 archaeal chromosomes, 59 archaeal plasmids, 1379 bacterial chromosomes, and 854 bacterial plasmids. In more than 80% of archaeal and bacterial plasmids, the GC content was lower than that of the host chromosome. Furthermore, most of the differences in GC content found between a plasmid and its host chromosome were less than 10%, and the GC content in plasmids and host chromosomes was highly correlated (Pearson’s correlation coefficient in bacteria and 0.917 in archaea). These

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Asensi, Marise Dutra, Arudy Penna Costa, Eliane Moura Falavina dos Reis, and Ernesto Hofer. "Lysotypes and plasmidial profile of Salmonella Serovar Typhimurium isolated from children with enteric processes in the cities of Rio de Janeiro, RJ, and Salvador, BA - Brazil." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 4 (1995): 297–302. http://dx.doi.org/10.1590/s0036-46651995000400003.

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The lysotypes, plasmidial profiles, and profiles of resistance to antimicrobial agents were determined in 111 Salmonella Typhimurium strains isolated from feces and blood of children treated in Rio de Janeiro and in Salvador. Six distinct lysotypes (19, 41, 97, 105, 120 and 193) were recognized, with a predominance of lysotype 193 (59.7%) in Rio de Janeiro and of phage type 105 (38.4) in Salvador. Approximately 86.7% of the lysotype 193 strains presented multiple resistance to more than six antimicrobial agents, whereas 93% of lysotype 105 strains were fully susceptible. More than 90% of the s

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Wilt, G. R., Gin Wu, R. C. Bird, and M. Toivio-Kinnucan. "Plasmid content of piliated and nonpiliated forms of Moraxella bovis." American Journal of Veterinary Research 51, no. 1 (1990): 171–73. http://dx.doi.org/10.2460/ajvr.1990.51.01.171.

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SUMMARY Plasmid profiles were compared between nonpiliated and piliated forms of Moraxella bovis isolates. The piliated form of M bovis isolate IBH64 contained 1 fewer plasmid than did the nonpiliated form. Piliated and nonpiliated cells of IBH64 contained plasmids having molecular size of 45, 32.8, 4.9, and 4.6 kilobases (kb). Single- and double-restriction endonuclease digestion by Ava I and Nde I indicated that the size of the additional plasmid carried by the nonpiliated form of IBH64 was approximately 43.6 kb. The M bovis isolates, Newport and GRS, contained the same number of plasmids in

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Penot, Isabelle, Nathalie Berges, Christine Guinguene, and Jacques Fages. "Characterization of Azospirillum associated with maize (Zea mays) in France, using biochemical tests and plasmid profiles." Canadian Journal of Microbiology 38, no. 8 (1992): 798–803. http://dx.doi.org/10.1139/m92-130.

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Thirty Azospirillum strains were isolated from the rhizosphere of 13 maize (Zea mays L.) cultivars grown in 14 French soils, using a new specific method, which has been given the name ROSEA. Among these strains 26 were Azospirillum lipoferum and 4 were Azospirillum brasilense. Their characterization was achieved using biochemical tests and plasmid profiles. Biochemical patterns allowed clear differentiation between the two species. A large diversity in carbon source metabolism was found among the Azospirillum sp. strains regardless of their origin. The A. brasilense were much more closely rela

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He, Dandan, Yingying Zhu, Ruichao Li, et al. "Emergence of a hybrid plasmid derived from IncN1-F33:A−:B− and mcr-1-bearing plasmids mediated by IS26." Journal of Antimicrobial Chemotherapy 74, no. 11 (2019): 3184–89. http://dx.doi.org/10.1093/jac/dkz327.

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Abstract Objectives To characterize the complete sequences of four plasmids in MCR-1-producing clinical Escherichia coli strain D72, and to depict the formation mechanism and characteristics of the cointegrate plasmid derived from the pD72-mcr1 and pD72-F33 plasmids. Methods The genetic profiles of plasmids in strain D72 and its transconjugant were determined by conjugation, S1-PFGE, Southern hybridization, WGS analysis and PCR. Plasmid sequences were analysed with bioinformatic tools. The traits of the fusion plasmid were characterized by cointegration, stability and conjugation assays. Resul

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Sadeghifard, Nourkhoda, Reza Ranjbar, Javad Zaeimi, et al. "Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria." Asian Biomedicine 4, no. 6 (2010): 901–11. http://dx.doi.org/10.2478/abm-2010-0118.

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Abstract Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing. Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university

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Olukoya, D. K., and O. Oni. "Plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from Nigeria." Epidemiology and Infection 105, no. 1 (1990): 59–64. http://dx.doi.org/10.1017/s0950268800047646.

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SUMMARYIn an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in Lagos, Nigeria was done. All the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. The commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. All but 4 of 100 isolates screened contained one or more plasmids. Plasmid profile analysis distinguished more strains than did antimicrobial susceptibility patterns. A total of 36 isolates was able to transfer resistanc

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Roig, Francisco J., and Carmen Amaro. "Plasmid diversity in Vibrio vulnificus biotypes." Microbiology 155, no. 2 (2009): 489–97. http://dx.doi.org/10.1099/mic.0.023424-0.

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Vibrio vulnificus is a heterogeneous bacterial species that can be virulent for humans and fish. Virulence in fish seems to rely on a recently described plasmid that can be transmitted between strains, aided by a conjugative plasmid. The main objective of this work was to analyse the plasmid content of a wide collection of strains from the three biotypes of the species, as well as to identify putative conjugative and virulence plasmids by means of Southern hybridization with specific probes and sequence analysis of selected gene markers. We found 28 different plasmid profiles in a total of 112

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Martin, Laura C., Emily K. Weir, Cornelis Poppe, Richard J. Reid-Smith, and Patrick Boerlin. "Characterization ofblaCMY-2Plasmids in Salmonella and Escherichia coli Isolates from Food Animals in Canada." Applied and Environmental Microbiology 78, no. 4 (2011): 1285–87. http://dx.doi.org/10.1128/aem.06498-11.

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ABSTRACTOne hundred thirty-fourblaCMY-2plasmids fromSalmonellaandEscherichia colistrains from animals and food in Canada were characterized. Five plasmid groups were identified based on replicon type and restriction profiles. Three groups containedE. coliplasmids only. IncA/C plasmids included most multiresistant plasmids and all those of bovine origin.

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Wang, Chun Guang, Tie Zhang, and Xing Hua Zhao. "Plasmid Fingerprinting Profiles of Chicken-Derived Pathogenic Strains of Escherichia coli." Advanced Materials Research 178 (December 2010): 355–60. http://dx.doi.org/10.4028/www.scientific.net/amr.178.355.

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Plasmid DNA was extracted from all 38 strains of chicken Escherichia coli by alkaline lysis method. The agarose gel electrophoresis showed 23 plasmid patterns. The strains from the same source had the same, similar, or quite different plasmid profiles, while those from different sources generally did not have the same plasmid profiles. Therefore, the plasmid profiles can provide basic materials for epidemiological investigation on chicken Escherichia coli at molecular level.

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Miljkovic-Selimovic, Biljana, Zorica Lepsanovic, Tatjana Babic, Branislava Kocic, and Gordana Randjelovic. "Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis." Vojnosanitetski pregled 65, no. 4 (2008): 303–7. http://dx.doi.org/10.2298/vsp0804303m.

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Background/Aim. As illness caused by Sallmonella enterica serovar Enteritidis (S. Enteritidis) occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. To determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of S. Enteritidis, recovered from patient's stools and food associated with outbreaks and those isolated from sporadic cases of diarrhea, were investigated. Methods. Investigatio

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Ling, J. M., P. C. Shaw, K. M. Kam, A. F. Cheng, and G. L. French. "Molecular studies of plasmids of multiply-resistantShigellaspp. in Hong Kong." Epidemiology and Infection 110, no. 3 (1993): 437–46. http://dx.doi.org/10.1017/s095026880005086x.

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SUMMARYOne hundred and twoShigellaspp. isolated in two hospitals in Hong Kong were analysed for antibiotic resistances, resistance plasmids and plasmid profiles. Three quarters of the isolates wereS.flexneri. All isolates harboured plasmids, up to a maximum of ten within one strain. Plasmids of 220 kb encoding resistances to tetracycline, chloramphenicol and sulphonamide and probably also associated with invasivenes in the Sereny test were found in 80 strains and were transferable in 18% of cases. Resistance plasmids of 92 and 99 kb were found in 27 and 15 strains respectively and encoded resi

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Zubrzycki, Igor Z., and Horst H. Klump. "Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs." Zeitschrift für Naturforschung C 50, no. 9-10 (1995): 652–55. http://dx.doi.org/10.1515/znc-1995-9-1008.

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Abstract High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

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Hartmann, A., and N. Amarger. "Genotypic diversity of an indigenous Rhizobium meliloti field population assessed by plasmid profiles, DNA fingerprinting, and insertion sequence typing." Canadian Journal of Microbiology 37, no. 8 (1991): 600–608. http://dx.doi.org/10.1139/m91-102.

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One hundred and twenty-five Rhizobium meliloti isolates nodulating field-grown Medicago sativa cv. Europe were evaluated for plasmid content and for symbiotic effectiveness with this host. Relative to the strain used commercially in France (RCR2011), isolates showed a comparable level of symbiotic effectiveness, although two isolates were poorly effective. Twenty-four distinct plasmid profiles were observed among these isolates, with a plasmid number varying between one and five per isolate. All isolates but one harboured a plasmid equivalent in size to that of the R. meliloti 41 megaplasmid.

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Tonin, Patricia N., and Robert B. Grant. "Genetic and physical characterization of trimethoprim resistance plasmids from Shigella sonnei and Shigella flexneri." Canadian Journal of Microbiology 33, no. 10 (1987): 905–13. http://dx.doi.org/10.1139/m87-157.

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Analysis of six Shigella flexneri and four S. sonnei isolates with trimethoprim (Tp) resistance from clinical cases in Ontario has shown that, in all isolates, the Tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. The physical and genetic characterization of these plasmids revealed that there are three different Tp resistance plasmids. One group, composed of all six S. flexneri plasmids, consists of plasmids which are about 70 megadaltons (MDa) and inhibit the fertility of an Escherichia coli Hfr strain (Fi+). A representative member of this group, p

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Elmagdub, Fathea E., Nuria A. Elamri, and Abdunabi M. Abughania. "Plasmid Profiles of Pseudomonas syringae pv. savastanoi." Journal of Misurata University for Agricultural Sciences, no. 01 (October 6, 2019): 354–78. http://dx.doi.org/10.36602/jmuas.2019.v01.01.28.

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Pseudomonas syringae pv. savastanoi (Psv) is the causal agent of olive knot disease. Forty nine bacterial isolates of Psv were isolated from knots on several hosts at the western area of Libya: 31 isolates from olive Olea europaea, 17 isolates from athel Tamarix aphylla (on which the disease is documented for the first time) and one isolate from retem Retama raetam. The isolates were identified on the basis of their morphological characteristics and LOPAT profile. They produced round, white creamy colonies on selective media (PAB and KB), from which 15 isolates produced fluorescent pigments. W

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Hynes, Michael F., and Michael P. O'Connell. "Host plant effect on competition among strains of Rhizobium leguminosarum." Canadian Journal of Microbiology 36, no. 12 (1990): 864–69. http://dx.doi.org/10.1139/m90-150.

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Analysis of plasmid profiles was used to type Rhizobium leguminosarum biovar viciae strains isolated from nodules of peas, lentils and faba beans grown in two different soils. One soil was from a native pasture with no previous history of cultivation, the other was from a plot in a rotation study which included lentils every 2 years. The results indicated a strong preference of both peas and faba beans for strains having certain specific plasmid profiles. Strains belonging to one plasmid profile group (group 2) formed over half the nodules on peas grown in soil from the rotation plot but were

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Lin, Yii-Lih, Tsegaye Sewunet, Sriram KK, Christian G. Giske, and Fredrik Westerlund. "Optical maps of plasmids as a proxy for clonal spread of MDR bacteria: a case study of an outbreak in a rural Ethiopian hospital." Journal of Antimicrobial Chemotherapy 75, no. 10 (2020): 2804–11. http://dx.doi.org/10.1093/jac/dkaa258.

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Abstract Objectives MDR bacteria have become a prevailing health threat worldwide. We here aimed to use optical DNA mapping (ODM) as a rapid method to trace nosocomial spread of bacterial clones and gene elements. We believe that this method has the potential to be a tool of pivotal importance for MDR control. Methods Twenty-four Escherichia coli samples of ST410 from three different wards were collected at an Ethiopian hospital and their plasmids were analysed by ODM. Plasmids were specifically digested with Cas9 targeting the antibiotic resistance genes, stained by competitive binding and co

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Sørum, Henning, Egil Myhr, Betty M. Zwicker, and Atle Lillehaug. "Comparison by Plasmid Profiling of Vibrio salmonicida Strains Isolated from Diseased Fish from Different North European and Canadian Coastal Areas of the Atlantic Ocean." Canadian Journal of Fisheries and Aquatic Sciences 50, no. 2 (1993): 247–50. http://dx.doi.org/10.1139/f93-028.

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Strains of Vibrio salmonicida from four different geographical areas, Norway, the Shetland Islands, the Faroe Islands, and eastern Canada, with coastlines bordering the North Atlantic, were subjected to plasmid DNA profiling after morphological, physiological, and serological classification. The strains of V. salmonicida from all these areas had common plasmid profiles, with the three plasmids of 21, 3.4, and 2.8 MDa as the most prevalent. The country of origin did not seem to be related to differences among the strains. Plasmid DNA profiling differentiates V. salmonicida isolates originating

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Lavollay, M., K. Mamlouk, T. Frank та ін. "Clonal Dissemination of a CTX-M-15 β-Lactamase-Producing Escherichia coli Strain in the Paris Area, Tunis, and Bangui". Antimicrobial Agents and Chemotherapy 50, № 7 (2006): 2433–38. http://dx.doi.org/10.1128/aac.00150-06.

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ABSTRACT One hundred twenty CTX-M-15-producing Escherichia coli strains isolated in 10 different hospitals from Paris (France), in the Hospital Charles Nicolle in Tunis (Tunisia), and in the Pasteur Institute in Bangui, Central African Republic (CAR), between 2000 and 2004 were studied. Eighty isolates, recovered from the three countries, were clonally related by repetitive extragenic palindromic PCR and pulsed-field gel electrophoresis. Various resistance profiles were identified among these clonal strains. After conjugation or electroporation of plasmids from E. coli strains representative o

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DD Moro. "Antibiotic susceptibility pattern and plasmid profiles of nasal staphylococci from apparently healthy Nigerians." World Journal of Biology Pharmacy and Health Sciences 6, no. 1 (2021): 019–25. http://dx.doi.org/10.30574/wjbphs.2021.6.1.0035.

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A total of 480 nasal samples from apparently healthy Nigerian students were collected aseptically and analyzed bacteriologically. Staphylococci were recovered from 432 (90%) of the subjects, constituting 288 (66.7%) and 144 (33.3%) of S. aureus and S. epidermidis respectively. The in-vitro antibiotic susceptibility testing using the disc diffusion technique showed high multiple resistance to the most commonly used antibiotics by Staphylococcus aureus such as penicillin (98.6%), ampicillin (97.2%), tetracycline (95.8%) and streptomycin (84.7%), but less resistance to erythromycin (9.7%), roceph

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Baggesen, D. L., D. Sandvang, and F. M. Aarestrup. "Characterization of Salmonella entericaSerovar Typhimurium DT104 Isolated from Denmark and Comparison with Isolates from Europe and the United States." Journal of Clinical Microbiology 38, no. 4 (2000): 1581–86. http://dx.doi.org/10.1128/jcm.38.4.1581-1586.2000.

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A total of 136 isolates of Salmonella enterica serovar Typhimurium DT104 from Denmark (n = 93), Germany (n = 10), Italy (n = 4), Spain (n = 5), and the United Kingdom (n = 9) were characterized by antimicrobial resistance analysis, plasmid profiling, pulsed-field gel electrophoresis (PFGE) with the restriction enzymes XbaI and BlnI, and analysis for the presence of integrons and antibiotic resistance genes. The isolates from Denmark were from nine pig herds, while the isolates from other countries were both of animal and of human origin. All but 10 isolates were resistant to ampicillin, chlora

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Isichei-Ukah, O. B., P. Ajuebor, and B. A. Omogbai. "Bacteriological Analysis and Plasmid Profiles of Surfaces of Some Hospital Kitchen Equipment in Benin City, Nigeria." Journal of Applied Sciences and Environmental Management 28, no. 3 (2024): 795–801. http://dx.doi.org/10.4314/jasem.v28i3.20.

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The surfaces of hospital kitchen equipment could be a major source of transmission of resistant pathogens to patients. Hence, the objective of this paper was to assess the bacteriological and plasmid profiles of surfaces of tables, sinks, chopping-boards, gas cookers and freezer handles hospital kitchen equipment in Benin City, Nigeria using appropriate standard microbiological techniques. Bacterial plasmids were isolated and separated using the agarose gel electrophoresis. Plasmid curing was performed using acridine orange. The antimicrobial sensitivity pattern showed that the bacterial isola

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Rastogi, V. K., E. S. P. Bromfield, S. T. Whitwill, and L. R. Barran. "A cryptic plasmid of indigenous Rhizobium meliloti possesses reiterated nodC and nifE genes and undergoes DNA rearrangement." Canadian Journal of Microbiology 38, no. 6 (1992): 563–68. http://dx.doi.org/10.1139/m92-092.

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Indigenous Rhizobium meliloti were previously characterized on the basis of plasmid profiles and phage sensitivity patterns (phage types). Rhizobium meliloti 1076, which contained two cryptic plasmids, was one of four isolates comprising phage type 23. In this study, the large cryptic plasmid pVS1(size >500 b) was transferred from isolate 1076 into the plasmid-free strain of Agrobacterium tumefaciens UBAPF1. This plasmid contained nucleotide sequences homologous to genes for nodulation (nodB, nodC) and nitrogen fixation (nifH, nifD, nifK, nifE). Cosmid clones possessing the nod and nif homo

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BEN AISSA, R., and N. AL-GALLAS. "Molecular typing ofSalmonella entericaserovars Enteritidis, Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001–2004." Epidemiology and Infection 136, no. 4 (2007): 468–75. http://dx.doi.org/10.1017/s0950268807008916.

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SUMMARYDuring the period from 2001 to 2004, a total of 72 isolates ofSalmonella entericaserovars: Anatum (n=40), Enteritidis (n=18), Corvallis (n=8), and Typhimurium (n=6), of various origins (mainly food and diarrhoeagenic stool samples), were collected and further characterized by antibiotic resistance, plasmid analysis, and pulsed-field gel electrophoresis (PFGE). Forty-five isolates presented multidrug resistance to antibiotics. Among which oneS. entericaserovar Anatum isolate was resistant to 11 antibiotics, and oneS. entericaserovar Typhimurium DT104 isolate was resistant to eight antibi

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Herchline, Thomas, and William Maher. "Plasmid Analysis of 26 Staphylococcal Species by a Rapid Microscale Technique." Infection Control & Hospital Epidemiology 17, no. 10 (1996): 687–90. http://dx.doi.org/10.1017/s0195941700003040.

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AbstractObjective:Evaluate a plasmid typing technique for a diverse group of staphylococci.Design:In vitro testing on known isolates.Setting:University hospital.Intervention:The plasmid content of 195 isolates representing 26 staphylococcal species was analyzed by agarose gel electrophoresis following lysostaphin-alkaline-SDS lysis, with and without acetone treatment.Results:Isolates yielded plasmid profiles with 0 to 7 extra-chromosomal bands (median, 1; 1.5); 171 (88%) had a profile with at least 1 band. Species with more than one isolate available for testing showed considerable diversity o

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Ling, J. M., I. C. Koo, K. M. Kam, and A. F. Cheng. "Antimicrobial Susceptibilities and Molecular Epidemiology of Salmonella enterica Serotype Enteritidis Strains Isolated in Hong Kong from 1986 to 1996." Journal of Clinical Microbiology 36, no. 6 (1998): 1693–99. http://dx.doi.org/10.1128/jcm.36.6.1693-1699.1998.

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The incidence of salmonellosis has been increasing in Hong Kong since 1989. The most common Salmonella enterica serotype isolated in 1994 was S. enteritidis. The antimicrobial susceptibilities and molecular epidemiology of 275 S. enteritidis strains isolated in this locality between 1986 and 1996 were studied. Over 99% of the isolates were susceptible to 17 of the 19 antimicrobial agents tested. One isolate harbored an autotransferring plasmid that confers resistance to tetracycline and trimethoprim-sulfamethoxazole. Another isolate harbored a mobilizable plasmid that confers resistance to amp

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DD, Moro. "Antibiotic susceptibility pattern and plasmid profiles of nasal staphylococci from apparently healthy Nigerians." World Journal of Biology Pharmacy and Health Sciences 6, no. 1 (2021): 019–25. https://doi.org/10.5281/zenodo.4767477.

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A total of 480 nasal samples from apparently healthy Nigerian students were collected aseptically and analyzed bacteriologically. Staphylococci were recovered from 432 (90%) of the subjects, constituting 288 (66.7%) and 144 (33.3%) of <em>S. aureus </em>and <em>S. epidermidis </em>respectively. The <em>in-vitro </em>antibiotic susceptibility testing using the disc diffusion technique showed high multiple resistance to the most commonly used antibiotics by <em>Staphylococcus aureus </em>such as penicillin (98.6%), ampicillin (97.2%), tetracycline (95.8%)

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47

Arturo, M., Z. Tamanai-Shacoori, C. Mamez, M. Pommepuy, and M. Cormier. "Two-dimensional electrophoresis method used for determination of plasmid profiles of Escherichia coli isolated from a sewage treatment plant." Canadian Journal of Microbiology 39, no. 10 (1993): 990–93. http://dx.doi.org/10.1139/m93-150.

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The plasmid contents of 306 strains of Escherichia coli isolated from raw and treated sewage from a treatment plant as well as from the water 1 km downstream were determined. The number and molecular weight of plasmids isolated from a representative sample of these strains were also measured. It was observed that biological treatment did not significantly alter these parameters. In contrast, statistically significant differences in plasmid number and size were observed when strains from sewage (raw and treated) were compared with strains isolated from brackish water. In raw and treated sewage,

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Hazen, Tracy H., LiCheng Zhao, Mallory A. Boutin, et al. "Comparative Genomics of an IncA/C Multidrug Resistance Plasmid from Escherichia coli and Klebsiella Isolates from Intensive Care Unit Patients and the Utility of Whole-Genome Sequencing in Health Care Settings." Antimicrobial Agents and Chemotherapy 58, no. 8 (2014): 4814–25. http://dx.doi.org/10.1128/aac.02573-14.

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ABSTRACTThe IncA/C plasmids have been implicated for their role in the dissemination of β-lactamases, including gene variants that confer resistance to expanded-spectrum cephalosporins, which are often the treatment of last resort against multidrug-resistant, hospital-associated pathogens. AblaFOX-5gene was detected in 14Escherichia coliand 16Klebsiellaisolates that were cultured from perianal swabs of patients admitted to an intensive care unit (ICU) of the University of Maryland Medical Center (UMMC) in Baltimore, MD, over a span of 3 years. Four of the FOX-encoding isolates were obtained fr

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Udo, Edet E., and Eiman Sarkhoo. "Genetic analysis of high-level mupirocin resistance in the ST80 clone of community-associated meticillin-resistant Staphylococcus aureus." Journal of Medical Microbiology 59, no. 2 (2010): 193–99. http://dx.doi.org/10.1099/jmm.0.013268-0.

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Four community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) isolates expressing high-level mupirocin resistance (MIC >1024 mg l−1) were isolated from four sites of a diabetic patient and characterized for the genetic location of their resistance determinants and typed using PFGE, staphylococcal cassette chromosome mec (SCCmec), the coagulase gene and multilocus sequence typing to ascertain their relatedness. The presence of genes for resistance to high-level mupirocin (mupA), tetracycline (tetK) and fusidic acid (far1), Panton–Valentine leukocidin (PVL), accessory gene re

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Boyce, John R., and Raymond L. Morter. "Plasmid profile analysis of bovine isolates of Pasteurella haemolytica." American Journal of Veterinary Research 47, no. 6 (1986): 1204–6. https://doi.org/10.2460/ajvr.1986.47.06.1204.

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SUMMARY Pasteurella haemolytica isolates, obtained from cattle with respiratory tract disease, were characterized as to serotype, antimicrobial susceptibility, and plasmid content. Strains isolated from 2 groups of cattle were compared. Remarkable similarity was seen in the plasmid profiles of isolates of the same serotype. In contrast, isolates of 2 different serotypes had totally different plasmid profiles.

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