Academic literature on the topic 'Plasmide F'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Plasmide F.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Plasmide F"

1

Lin, Mei-Hui, and Shih-Tung Liu. "Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System." Journal of Bacteriology 190, no. 10 (2008): 3681–89. http://dx.doi.org/10.1128/jb.00846-07.

Full text
Abstract:
ABSTRACT Plasmid pSW100 is 1 of the 13 plasmids from Pantoea stewartii subsp. stewartii SW2 which has a replicon that resembles that of ColE1. This work uses a pSW100 derivative, pSW140K, to study how the pSW100 replicon is stably maintained in its hosts. Our results indicate that although pSW140K is stable in Escherichia coli HB101, the plasmid is rapidly lost in another E. coli strain, DH5α, indicating that the genetic background of an E. coli strain affects the stability of pSW140K. Mutagenesis of E. coli HB101 with EZ::TN <DHFR-1> revealed that mutations in traC, traF, traG, traN, an
APA, Harvard, Vancouver, ISO, and other styles
2

Matlock, William, Kevin K. Chau, Manal AbuOun, et al. "Genomic network analysis of environmental and livestock F-type plasmid populations." ISME Journal 15, no. 8 (2021): 2322–35. http://dx.doi.org/10.1038/s41396-021-00926-w.

Full text
Abstract:
AbstractF-type plasmids are diverse and of great clinical significance, often carrying genes conferring antimicrobial resistance (AMR) such as extended-spectrum β-lactamases, particularly in Enterobacterales. Organising this plasmid diversity is challenging, and current knowledge is largely based on plasmids from clinical settings. Here, we present a network community analysis of a large survey of F-type plasmids from environmental (influent, effluent and upstream/downstream waterways surrounding wastewater treatment works) and livestock settings. We use a tractable and scalable methodology to
APA, Harvard, Vancouver, ISO, and other styles
3

Boyd, E. Fidelma, Charles W. Hill, Stephen M. Rich, and Daniel L. Hartl. "Mosaic Structure of Plasmids From Natural Populations of Escherichia coli." Genetics 143, no. 3 (1996): 1091–100. http://dx.doi.org/10.1093/genetics/143.3.1091.

Full text
Abstract:
Abstract The distribution of plasmids related to the fertility factor F was examined in the ECOR reference collection of Escherichia coli. Probes specific for four F-related genes were isolated and used to survey the collection by DNA hybridization. To estimate the genetic diversity of genes in F-like plasmids, DNA sequences were obtained for four plasmid genes. The phylogenetic relationships among the plasmids in the ECOR strains is very different from that of the strains themselves. This finding supports the view that plasmid transfer has been frequent within and between the major groups of
APA, Harvard, Vancouver, ISO, and other styles
4

Gardner, Murray N., Shelly M. Deane, and Douglas E. Rawlings. "Isolation of a New Broad-Host-Range IncQ-Like Plasmid, pTC-F14, from the Acidophilic Bacterium Acidithiobacillus caldus and Analysis of the Plasmid Replicon." Journal of Bacteriology 183, no. 11 (2001): 3303–9. http://dx.doi.org/10.1128/jb.183.11.3303-3309.2001.

Full text
Abstract:
ABSTRACT A moderately thermophilic (45 to 50°C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic Acidithiobacillus caldusstrain, f, was isolated from a biooxidation process used to treat nickel ore. Trans-alternating field electrophoresis analysis of total DNA from the A. caldus cells revealed two plasmids of approximately 14 and 45 kb. The 14-kb plasmid, designated pTC-F14, was cloned and shown by replacement of the cloning vector with a kanamycin resistance gene to be capable of autonomous replication inEscherichia coli. Autonomous replication was also demonstrated in Pseudomonas putid
APA, Harvard, Vancouver, ISO, and other styles
5

Haake, Susan Kinder, Sean C. Yoder, Gwynne Attarian, and Kara Podkaminer. "Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems." Journal of Bacteriology 182, no. 4 (2000): 1176–80. http://dx.doi.org/10.1128/jb.182.4.1176-1180.2000.

Full text
Abstract:
ABSTRACT Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.
APA, Harvard, Vancouver, ISO, and other styles
6

Hammerl, Jens A., Iris Klein, Erich Lanka, Bernd Appel, and Stefan Hertwig. "Genetic and Functional Properties of the Self-Transmissible Yersinia enterocolitica Plasmid pYE854, Which Mobilizes the Virulence Plasmid pYV." Journal of Bacteriology 190, no. 3 (2007): 991–1010. http://dx.doi.org/10.1128/jb.01467-07.

Full text
Abstract:
ABSTRACT Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer
APA, Harvard, Vancouver, ISO, and other styles
7

Dionisio, Francisco, Ivan Matic, Miroslav Radman, Olivia R. Rodrigues, and François Taddei. "Plasmids Spread Very Fast in Heterogeneous Bacterial Communities." Genetics 162, no. 4 (2002): 1525–32. http://dx.doi.org/10.1093/genetics/162.4.1525.

Full text
Abstract:
Abstract Conjugative plasmids can mediate gene transfer between bacterial taxa in diverse environments. The ability to donate the F-type conjugative plasmid R1 greatly varies among enteric bacteria due to the interaction of the system that represses sex-pili formations (products of finOP) of plasmids already harbored by a bacterial strain with those of the R1 plasmid. The presence of efficient donors in heterogeneous bacterial populations can accelerate plasmid transfer and can spread by several orders of magnitude. Such donors allow millions of other bacteria to acquire the plasmid in a matte
APA, Harvard, Vancouver, ISO, and other styles
8

Haryanto, Aris, Hevi Wihadmadyatami, and Nastiti Wijayanti. "In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system." Indonesian Journal of Biotechnology 25, no. 2 (2020): 69. http://dx.doi.org/10.22146/ijbiotech.54703.

Full text
Abstract:
The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV). The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmid
APA, Harvard, Vancouver, ISO, and other styles
9

van Zyl, Leonard J., Shelly M. Deane, Lilly-Ann Louw, and Douglas E. Rawlings. "Presence of a Family of Plasmids (29 to 65 Kilobases) with a 26-Kilobase Common Region in Different Strains of the Sulfur-Oxidizing Bacterium Acidithiobacillus caldus." Applied and Environmental Microbiology 74, no. 14 (2008): 4300–4308. http://dx.doi.org/10.1128/aem.00864-08.

Full text
Abstract:
ABSTRACT Three large cryptic plasmids from different isolates of Acidithiobacillus caldus were rescued by using an in vitro transposition system that delivers a kanamycin-selectable marker and an Escherichia coli plasmid origin of replication. The largest of the plasmids, the 65-kb plasmid pTcM1, was isolated from a South African A. caldus strain, MNG. This plasmid was sequenced and compared to that of pTcF1 (39 kb, from strain “f,” South Africa) and pC-SH12 (29 kb, from strain C-SH12, Australia). With the exception of a 2.7-kb insertion sequence, pC-SH12 appears to represent the DNA common to
APA, Harvard, Vancouver, ISO, and other styles
10

Fekete, Richard A., and Laura S. Frost. "Mobilization of Chimeric oriT Plasmids by F and R100-1: Role of Relaxosome Formation in Defining Plasmid Specificity." Journal of Bacteriology 182, no. 14 (2000): 4022–27. http://dx.doi.org/10.1128/jb.182.14.4022-4027.2000.

Full text
Abstract:
ABSTRACT Cleavage at the F plasmid nic site within the origin of transfer (oriT) requires the F-encoded proteins TraY and TraI and the host-encoded protein integration host factor in vitro. We confirm that F TraY, but not F TraM, is required for cleavage atnic in vivo. Chimeric plasmids were constructed which contained either the entire F or R100-1 oriT regions or various combinations of nic, TraY, and TraM binding sites, in addition to the traM gene. The efficiency of cleavage atnic and the frequency of mobilization were assayed in the presence of F or R100-1 plasmids. The ability of these ch
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Plasmide F"

1

Sommer, Suzanne. "Induction des fonctions SOS par introduction d'ADN exogène." Paris 11, 1987. http://www.theses.fr/1987PA112120.

Full text
Abstract:
Les bactéries réagissent aux traitements qui endommagent l'ADN par l'induction d'un ensemble de gènes dits "SOS". L'induction des gènes SOS résulte de l'inactivation du répresseur LexA par une forme activée de la protéine RecA. Quand la réplication de l'ADN est bloquée, un signal appelé signal SOS permet l'activation de la protéine RecA. 1- Pour déterminer la nature et les mécanismes de formation du signal SOS, nous avons introduit dans des cellules intactes des réplicons endommagés et/ou bloqués dans leur réplication. Nos résultats montrent que le signal SOS est constitué par des portions d'A
APA, Harvard, Vancouver, ISO, and other styles
2

Ah-Seng, Yoan. "La Ségrégation du plasmide F d'Escherichia coli : régulation de l'activité ATPase de la protéine moteur de partition SopA." Toulouse 3, 2010. http://thesesups.ups-tlse.fr/1126/.

Full text
Abstract:
La ségrégation, ou partition, des chromosomes et des plasmides bactériens est l'étape fondamentale du cycle cellulaire qui assure la transmission de l'ensemble du génome aux cellules filles. C'est l'équivalent procaryote de la mitose. Des systèmes de ségrégation, appelés les loci par, ont été identifiés sur les plasmides à bas nombre de copies, et des homologues de ces systèmes de partition sont présents sur la majorité des chromosomes bactériens. Le système code deux protéines, une ATPase et une protéine qui se fixe spécifiquement sur une région centromérique. Ces deux protéines interagissent
APA, Harvard, Vancouver, ISO, and other styles
3

Castaing, Jean-Philippe. "La ségrégation du plasmide F d'Escherichia coli : étude du rôle de la fixation de l'ATPase Sopa à l'ADN." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/597/.

Full text
Abstract:
La ségrégation de l'ADN, appelée partition chez les procaryotes, permet à tout organisme de transmettre son patrimoine génétique au cours des générations. Il existe des systèmes actifs de partition présents sur la majorité des plasmides et des chromosomes bactériens. Ces systèmes sont essentiels pour la ségrégation active des plasmides à bas nombre de copies tel que le plasmide F d'Escherichia coli, étudié au sein de notre équipe. Son système de partition, appelé sop, est composé de deux gènes, sopA et sopB et d'une séquence centromérique sopC. SopB se fixe à sopC pour former le complexe de pa
APA, Harvard, Vancouver, ISO, and other styles
4

LEMONNIER, MARC. "Partition des plasmides a bas nombre de copies chez escherichia coli : etudes des interactions qui impliquent les elements sopabc du plasmide f." Toulouse 3, 1998. http://www.theses.fr/1998TOU30131.

Full text
Abstract:
Chez les bacteries, la fonction qui assure la distribution equitable des genomes dans chacune des cellules filles issues de la division cellulaire est appelee partition. Les acteurs et les mecanismes de ce processus analogue a la mitose eucaryote sont encore mal connus. Pour etudier la partition, notre modele est le plasmide f d'escherichia coli. Present a bas nombre de copies dans la cellule, il porte ses propres determinants de partition sous forme de deux genes codant pour les proteines sopa et sopb, et d'une sequence centromerique, sopc, avec laquelle sopb forme un complexe qui pourrait et
APA, Harvard, Vancouver, ISO, and other styles
5

Sanchez, Aurore. "La ségrégation du plasmide F d'Escherichia coli : étude des spécificités d'interaction du centromère avec la protéine SopB et organisation du complexe de partition étendu." Toulouse 3, 2014. http://thesesups.ups-tlse.fr/2447/.

Full text
Abstract:
La ségrégation du matériel génétique est une étape fondamentale du cycle cellulaire permettant la transmission du patrimoine génétique au cours des générations. Dans les cellules eucaryotes, la mitose est l'étape qui permet la répartition des chromosomes dupliqués dans chaque cellule fille. Des systèmes actifs, dédiés à la ségrégation de l'ADN sont retrouvés sur la majorité des plasmides et chromosomes bactériens. Ces systèmes ParABS, dits "de partition", sont constitués de deux protéines, ParA et ParB, et d'une séquence centromérique, parS. La protéine ParA est une ATPase capable de positionn
APA, Harvard, Vancouver, ISO, and other styles
6

Bernard, Philippe. "Etude génétique et biochimie du mécanisme CCD du plasmide F et de son interaction avec la gyrase." Doctoral thesis, Universite Libre de Bruxelles, 1993. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212820.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Bernard, Philippe. "Etude génétique et biochimique du mécanisme CCD du plasmide F et de son interaction avec la gyrase." Doctoral thesis, Universite Libre de Bruxelles, 1994. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212690.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Bahassi, El Mustapha. "Etude génétique et biochimique de la protéine CcdB du plasmide F et de son intéraction avec la gyrase." Doctoral thesis, Universite Libre de Bruxelles, 1996. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212373.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Salmon, Michel André. "Etude génétique et biochimique des propriétés 'poison-antidote' et régulatrices des protéines CcdA et CcdB du plasmide F de Escherichia coli." Doctoral thesis, Universite Libre de Bruxelles, 1993. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212783.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Libante, Virginie. "Analyse du système SOP responsable de la partition du plasmide F chez Escherichia coli : sopA, une ATPase essentielle au mécanisme de partition." Toulouse 3, 2002. http://www.theses.fr/2002TOU30209.

Full text
Abstract:
Le maintien des réplicons bactériens (partition) présente des similitudes avec la mitose eukaryote. Dans l'équipe du Dr Lane nous étudions le plasmide F d'Escherichia coli qui possède un système actif de partition. Ce dernier est très répandu parmi les réplicons bactériens. Il est composé de deux protéines, SopA et SopB produites à partir d'un opéron dont la transcription est réprimée par SopA. En aval se trouve le site sopC sur lequel SopB se fixe pour former le complexe de partition. Nous avons centrés nos recherches sur l'activité ATPase de SopA au moyen d'une mutagenèse du site actif de fi
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Plasmide F"

1

Schindler, Thomas E. A Hidden Legacy. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780197531679.001.0001.

Full text
Abstract:
This biography of Esther Zimmer Lederberg highlights the importance of her research work, which revealed the unique features of bacterial sex, essential for our understanding of molecular biology and evolution. A Hidden Legacy relates how, she and her husband Joshua Lederberg established the new field of bacterial genetics together, in the decade leading up to the discovery of the DNA double helix. Their impressive series of achievements include: the discovery of λ‎ bacteriophage and of the first plasmid, known as the F-factor; the demonstration that viruses carry bacterial genes between bacte
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Plasmide F"

1

Gooch, Jan W. "F Plasmid." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13787.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Gooch, Jan W. "F´ Plasmid." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13790.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Mullineaux, Phil, and Neil Willetts. "Promoters in the Transfer Region of Plasmid F." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_42.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Austin, Stuart, and Ann Abeles. "The Partition Functions of P1, P7, and F Miniplasmids." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_18.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Hiraga, Sota, Teru Ogura, Hirotada Mori, and Masafumi Tanaka. "Mechanisms Essential for Stable Inheritance of Mini-F Plasmid." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Laine, Susan, Deanna Moore, Pushpa Kathir, and Karin Ippen-Ihler. "Genes and Gene Products Involved in the Synthesis of F-Pili." In Plasmids in Bacteria. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2447-8_38.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Birge, Edward A. "Plasmids and Conjugation Systems Other Than F." In Bacterial and Bacteriophage Genetics. Springer New York, 2000. http://dx.doi.org/10.1007/978-1-4757-3258-0_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Dempsey, Walter B. "Key Regulatory Aspects of Transfer of F-Related Plasmids." In Bacterial Conjugation. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Hamm, Naomi C., Andrew N. Stammers, Shanel E. Susser, et al. "Regulation of Cardiac Sarco(endo)plasmic Reticulum Calcium-ATPases (SERCA2a) in Response to Exercise." In Regulation of Ca2+-ATPases,V-ATPases and F-ATPases. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-24780-9_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Ippen-Ihler, Karin, and Ronald A. Skurray. "Genetic Organization of Transfer-Related Determinants on the Sex Factor F and Related Plasmids." In Bacterial Conjugation. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4757-9357-4_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Plasmide F"

1

KUROSO, K., S. IKEMATSU, M. HADA, M. FUJIMAKI, and K. FUKUTAKE. "DEVELOPMENT OF A NEW ASSAY METHOD FOR THE DETECTION OF DD/E COMPLEX." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643130.

Full text
Abstract:
A new assay method for the detection of DD/E complex derived from crosslinked fibrin is developed. This assay is performed on a microtitre plate using capture/tag antibody technique, in which the monoclonal antibody against D dimer fragment (DD-3B6, MAbCO) is coated and anti-E fragment polyclonal F(ab)’2 conjugated with horse radish peroxidase is for a tag-anti body. Antigen dilution curve is drawn in the range of 0.01-1.0 pg/ml of purified DD/E complex. DD/E complex can be measured specifically and other high molecular weight derivatives from crosslinked fibrin show a little crossreaction, th
APA, Harvard, Vancouver, ISO, and other styles
2

Seitz, R., M. Wolf, R. Egbring, and K. Havemann. "Neutrophil Elastase, Thrombin and Plasmin in Septic Shock: Influence on Prognosis." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643894.

Full text
Abstract:
The prognosis of septicaemia depends on the occurrence of disseminated disturbances of the microcirculation impairing organ function and haemorrhagic complications due to consumption of coagulation factors. The intravasal appearance of three potentially involved proteinases in active form can be detected by immunologic determination of their complexes with inhibitors: thrombin-antithrombin III (TAT), according to PELZER et al. (Thrombos. Haemostas. 54:24,1985); plasmin- antiplasmin (PAP), ldlE, antiserum donated by KARGES; human neutrophil elastase (HNE)- antitrypsin, ELISA, Merck, DarmstadtIn
APA, Harvard, Vancouver, ISO, and other styles
3

Murayama, H., and N. U. Bang. "INCORPORATION OF PLASMINOGEN ACTIVATOR INHIBITOR INTO FIBRIN, AN ALTERNATIVE REGULATORY PATHWAY OF FIBRINOLYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644442.

Full text
Abstract:
A plasminogen activator inhibitor (PAI-1) Mj, 50 kd is normally found in plasma at low concentrations. Plasma levels increase sharply upon stimulation of endothelial cells with endotoxin or monokines and activated platelets secrete significant quantities of PAI-1. It is possible that high levels of PAI-1 may be achieved at the local sites of intravascular thrombi. Semipurified PAI-1 was therefore prepared from human platelets to study its affinity for fibrin (F). Approximately 50% PAI-1 adsorbed to F monomer immobilized on sepharose and desorbed under conditions of acidic pH and high ionic str
APA, Harvard, Vancouver, ISO, and other styles
4

Verheijen, J. M., M. P. M. Caspers, G. A. W. de Munk, B. E. Enger-Valk, G. T. G. Chang, and P. H. Pouwels. "SITES IN TISSUE-TYPE PLASMINOGEN ACTIVATOR INVOLVED IN THE INTERACTION WITH FIBRIN, PLASMINOGEN AND LOW MOLECULAR WEIGHT LIGANDS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644613.

Full text
Abstract:
Tissue-type plasminogen activator (t-PA) activates the proenzyme plasminogen to the active protease plasmin which degrades fibrin. The unique properties of t-PA, fibrin binding and stimulation of activity by fibrin make it an interesting molecule for specific thrombolysis. t-PA is thought to consist of five structural regions designated finger (F), growth factor (G), kringle 1 (Kl), kringle 2 (K2) and protease (P). Previous studies have shown that the interaction of t-PA with fibrin is mediated by the F and K2 regions.Mutated t-PA cDNA molecules were expressed in Chinese hamster ovary cells an
APA, Harvard, Vancouver, ISO, and other styles
5

Goodwin, C. A., M. F. Scully, V. Ellis, and V. V. Kakkar. "THROMBIN BINDING FRAGMENT E GENERATED DURING FIBRINOGENOLYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642937.

Full text
Abstract:
Binding of fibrinogen by thrombin was measured by inhibition of amidolysis of S2238 and found to be 12 μM. Upon digestion of fibrinogen with plasmin (0.16ugs/mg fibrinogen) for 4 hours at 37°C, thrombin binding activity remained in the supernatants upon heat treatment. The thrombin binding activity in the dialyzed supernatant reached a maximum after two hours coinciding with maximal release of B 1-42 and 45-39 KDa chain fragments. Measured immunologically, levels of fragment E at this time were 45% of the maximum generated after 4 hours digestion. FPA levels in the dialyzed supernatant (measur
APA, Harvard, Vancouver, ISO, and other styles
6

Ibragimov, A. E., D. Yu Garshina, An Kh Baymiev, and O. V. Lastochkina. "Modulation of Triticum aestivum L. tolerance to combined abiotic/biotic stresses by endophytic plant growth promoting bacteria Bacillus subtilis." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.11.

Full text
Abstract:
Wheat (Triticum aestivum L.) is one of the most important cereal food crops worldwide. Various abiotic and biotic stresses or their combinations lead to crop losses (up to 50-82%) and pose a serious threat to the agricultural industry and food security. Plant growth-promoting endophytic bacteria Bacillus subtilis are considered as a bioactive and eco-friendly strategy for plant protection. Earlier, we have shown B. subtilis 10-4 has a growth-promoting and anti-stress effect on wheat under water deficiency. Here, we investigated the effect of B. subtilis 10-4 and B. subtilis 10-4+salicylic acid
APA, Harvard, Vancouver, ISO, and other styles
7

Liu, Chung Y., Per Wallen, Dean Handley, and Jena Smith. "FIERIN POTENTIATING THE ACTIVATION OF FIBRINOLYTIC SYSTEM ON THE ENDOTHELIAL CELL SURFACE: FORMATION OF THE SURFACE-BOUND TRIMOLECULAR COMPLEX OF FIBRIN, PLASMINOGEN, AND PLASMINOGEN ACTIVATOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643318.

Full text
Abstract:
Previous reports have shown that f ibrin (Fn), plasminogen (Pig), and tissue plasminogen activator (tPA) can bind to bovine aortic endothelial cell (BAEC) respectively. The present studies are to examine the formation of the trimolecular complex of Fn, Pig, and tPA on the BAEC surface. BAEC monolayers were first incubated with one of the three components in buffer (pH 7.5, 25 C) and washed, and then incubated with the second component and washed. Finally, the BAEC monolayers after the first and second incubations were incubated with the third component in the presence of plasmin substrate S-22
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!