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Journal articles on the topic 'Plasmide F'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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1

Lin, Mei-Hui, and Shih-Tung Liu. "Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System." Journal of Bacteriology 190, no. 10 (2008): 3681–89. http://dx.doi.org/10.1128/jb.00846-07.

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ABSTRACT Plasmid pSW100 is 1 of the 13 plasmids from Pantoea stewartii subsp. stewartii SW2 which has a replicon that resembles that of ColE1. This work uses a pSW100 derivative, pSW140K, to study how the pSW100 replicon is stably maintained in its hosts. Our results indicate that although pSW140K is stable in Escherichia coli HB101, the plasmid is rapidly lost in another E. coli strain, DH5α, indicating that the genetic background of an E. coli strain affects the stability of pSW140K. Mutagenesis of E. coli HB101 with EZ::TN <DHFR-1> revealed that mutations in traC, traF, traG, traN, an

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2

Matlock, William, Kevin K. Chau, Manal AbuOun, et al. "Genomic network analysis of environmental and livestock F-type plasmid populations." ISME Journal 15, no. 8 (2021): 2322–35. http://dx.doi.org/10.1038/s41396-021-00926-w.

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AbstractF-type plasmids are diverse and of great clinical significance, often carrying genes conferring antimicrobial resistance (AMR) such as extended-spectrum β-lactamases, particularly in Enterobacterales. Organising this plasmid diversity is challenging, and current knowledge is largely based on plasmids from clinical settings. Here, we present a network community analysis of a large survey of F-type plasmids from environmental (influent, effluent and upstream/downstream waterways surrounding wastewater treatment works) and livestock settings. We use a tractable and scalable methodology to

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3

Boyd, E. Fidelma, Charles W. Hill, Stephen M. Rich, and Daniel L. Hartl. "Mosaic Structure of Plasmids From Natural Populations of Escherichia coli." Genetics 143, no. 3 (1996): 1091–100. http://dx.doi.org/10.1093/genetics/143.3.1091.

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Abstract The distribution of plasmids related to the fertility factor F was examined in the ECOR reference collection of Escherichia coli. Probes specific for four F-related genes were isolated and used to survey the collection by DNA hybridization. To estimate the genetic diversity of genes in F-like plasmids, DNA sequences were obtained for four plasmid genes. The phylogenetic relationships among the plasmids in the ECOR strains is very different from that of the strains themselves. This finding supports the view that plasmid transfer has been frequent within and between the major groups of

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4

Gardner, Murray N., Shelly M. Deane, and Douglas E. Rawlings. "Isolation of a New Broad-Host-Range IncQ-Like Plasmid, pTC-F14, from the Acidophilic Bacterium Acidithiobacillus caldus and Analysis of the Plasmid Replicon." Journal of Bacteriology 183, no. 11 (2001): 3303–9. http://dx.doi.org/10.1128/jb.183.11.3303-3309.2001.

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ABSTRACT A moderately thermophilic (45 to 50°C), highly acidophilic (pH 1.5 to 2.5), chemolithotrophic Acidithiobacillus caldusstrain, f, was isolated from a biooxidation process used to treat nickel ore. Trans-alternating field electrophoresis analysis of total DNA from the A. caldus cells revealed two plasmids of approximately 14 and 45 kb. The 14-kb plasmid, designated pTC-F14, was cloned and shown by replacement of the cloning vector with a kanamycin resistance gene to be capable of autonomous replication inEscherichia coli. Autonomous replication was also demonstrated in Pseudomonas putid

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5

Haake, Susan Kinder, Sean C. Yoder, Gwynne Attarian, and Kara Podkaminer. "Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems." Journal of Bacteriology 182, no. 4 (2000): 1176–80. http://dx.doi.org/10.1128/jb.182.4.1176-1180.2000.

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ABSTRACT Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

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6

Hammerl, Jens A., Iris Klein, Erich Lanka, Bernd Appel, and Stefan Hertwig. "Genetic and Functional Properties of the Self-Transmissible Yersinia enterocolitica Plasmid pYE854, Which Mobilizes the Virulence Plasmid pYV." Journal of Bacteriology 190, no. 3 (2007): 991–1010. http://dx.doi.org/10.1128/jb.01467-07.

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ABSTRACT Yersinia strains frequently harbor plasmids, of which the virulence plasmid pYV, indigenous in pathogenic strains, has been thoroughly characterized during the last decades. Yet, it has been unknown whether the nonconjugative pYV can be transferred by helper plasmids naturally occurring in this genus. We have isolated the conjugative plasmids pYE854 (95.5 kb) and pYE966 (70 kb) from a nonpathogenic and a pathogenic Yersinia enterocolitica strain, respectively, and demonstrate that both plasmids are able to mobilize pYV. The complete sequence of pYE854 has been determined. The transfer

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7

Dionisio, Francisco, Ivan Matic, Miroslav Radman, Olivia R. Rodrigues, and François Taddei. "Plasmids Spread Very Fast in Heterogeneous Bacterial Communities." Genetics 162, no. 4 (2002): 1525–32. http://dx.doi.org/10.1093/genetics/162.4.1525.

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Abstract Conjugative plasmids can mediate gene transfer between bacterial taxa in diverse environments. The ability to donate the F-type conjugative plasmid R1 greatly varies among enteric bacteria due to the interaction of the system that represses sex-pili formations (products of finOP) of plasmids already harbored by a bacterial strain with those of the R1 plasmid. The presence of efficient donors in heterogeneous bacterial populations can accelerate plasmid transfer and can spread by several orders of magnitude. Such donors allow millions of other bacteria to acquire the plasmid in a matte

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8

Haryanto, Aris, Hevi Wihadmadyatami, and Nastiti Wijayanti. "In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system." Indonesian Journal of Biotechnology 25, no. 2 (2020): 69. http://dx.doi.org/10.22146/ijbiotech.54703.

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The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV). The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmid

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9

van Zyl, Leonard J., Shelly M. Deane, Lilly-Ann Louw, and Douglas E. Rawlings. "Presence of a Family of Plasmids (29 to 65 Kilobases) with a 26-Kilobase Common Region in Different Strains of the Sulfur-Oxidizing Bacterium Acidithiobacillus caldus." Applied and Environmental Microbiology 74, no. 14 (2008): 4300–4308. http://dx.doi.org/10.1128/aem.00864-08.

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ABSTRACT Three large cryptic plasmids from different isolates of Acidithiobacillus caldus were rescued by using an in vitro transposition system that delivers a kanamycin-selectable marker and an Escherichia coli plasmid origin of replication. The largest of the plasmids, the 65-kb plasmid pTcM1, was isolated from a South African A. caldus strain, MNG. This plasmid was sequenced and compared to that of pTcF1 (39 kb, from strain “f,” South Africa) and pC-SH12 (29 kb, from strain C-SH12, Australia). With the exception of a 2.7-kb insertion sequence, pC-SH12 appears to represent the DNA common to

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10

Fekete, Richard A., and Laura S. Frost. "Mobilization of Chimeric oriT Plasmids by F and R100-1: Role of Relaxosome Formation in Defining Plasmid Specificity." Journal of Bacteriology 182, no. 14 (2000): 4022–27. http://dx.doi.org/10.1128/jb.182.14.4022-4027.2000.

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ABSTRACT Cleavage at the F plasmid nic site within the origin of transfer (oriT) requires the F-encoded proteins TraY and TraI and the host-encoded protein integration host factor in vitro. We confirm that F TraY, but not F TraM, is required for cleavage atnic in vivo. Chimeric plasmids were constructed which contained either the entire F or R100-1 oriT regions or various combinations of nic, TraY, and TraM binding sites, in addition to the traM gene. The efficiency of cleavage atnic and the frequency of mobilization were assayed in the presence of F or R100-1 plasmids. The ability of these ch

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11

Lorenz, Sandra C., Steven R. Monday, Maria Hoffmann, Markus Fischer, and Julie A. Kase. "Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path." Applied and Environmental Microbiology 82, no. 21 (2016): 6367–77. http://dx.doi.org/10.1128/aem.01839-16.

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ABSTRACTMost Shiga toxin-producingEscherichia coli(STEC) strains associated with severe disease, such as hemolytic-uremic syndrome (HUS), carry large enterohemolysin-encoding (ehxA) plasmids, e.g., pO157 and pO103, that contribute to STEC clinical manifestations. SixehxAsubtypes (A through F) exist that phylogenetically cluster intoeae-positive (B, C, F), a mix ofeae-positive (E) andeae-negative (A), and a third, more distantly related, cluster ofeae-negative (D) STEC strains. While subtype B, C, and F plasmids share a number of virulence traits that are distinct from those of subtype A, seque

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Zienkiewicz, M., I. Kern-Zdanowicz, M. Gołȩbiewski та ін. "Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum β-Lactamase Gene blaSHV-5". Antimicrobial Agents and Chemotherapy 51, № 4 (2007): 1164–71. http://dx.doi.org/10.1128/aac.00772-06.

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ABSTRACT Escherichia coli isolates recovered from patients during a clonal outbreak in a Warsaw, Poland, hospital in 1997 produced different levels of an extended-spectrum β-lactamase (ESBL) of the SHV type. The β-lactamase hyperproduction correlated with the multiplication of ESBL gene copies within a plasmid. Here, we present the complete nucleotide sequence of plasmid p1658/97 carried by the isolates recovered during the outbreak. The plasmid is 125,491 bp and shows a mosaic structure in which all modules constituting the plasmid core are homologous to those found in plasmids F and R100 and

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13

Roig, Francisco J., and Carmen Amaro. "Plasmid diversity in Vibrio vulnificus biotypes." Microbiology 155, no. 2 (2009): 489–97. http://dx.doi.org/10.1099/mic.0.023424-0.

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Vibrio vulnificus is a heterogeneous bacterial species that can be virulent for humans and fish. Virulence in fish seems to rely on a recently described plasmid that can be transmitted between strains, aided by a conjugative plasmid. The main objective of this work was to analyse the plasmid content of a wide collection of strains from the three biotypes of the species, as well as to identify putative conjugative and virulence plasmids by means of Southern hybridization with specific probes and sequence analysis of selected gene markers. We found 28 different plasmid profiles in a total of 112

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14

Wilbaux, Myriam, Natacha Mine, Anne-Marie Guérout, Didier Mazel, and Laurence Van Melderen. "Functional Interactions between Coexisting Toxin-Antitoxin Systems of the ccd Family in Escherichia coli O157:H7." Journal of Bacteriology 189, no. 7 (2007): 2712–19. http://dx.doi.org/10.1128/jb.01679-06.

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ABSTRACT Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd F) located in the chromosomal backbone of the pathogenic O157:H7 Escherichia coli strain (ccd O157). The ccd F and the ccd O157 systems coexist in O157:H7 isolates, as these pathogenic strains contain an F-related virulence plasmid carrying the ccd F system. We have shown that the chromosomal ccd O157 system encodes functional toxin and a

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15

May, Thithiwat, and Satoshi Okabe. "Escherichia coli Harboring a Natural IncF Conjugative F Plasmid Develops Complex Mature Biofilms by Stimulating Synthesis of Colanic Acid and Curli." Journal of Bacteriology 190, no. 22 (2008): 7479–90. http://dx.doi.org/10.1128/jb.00823-08.

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ABSTRACT It has been shown that Escherichia coli harboring the derepressed IncFI and IncFII conjugative F plasmids form complex mature biofilms by using their F-pilus connections, whereas a plasmid-free strain forms only patchy biofilms. Therefore, in this study we investigated the contribution of a natural IncF conjugative F plasmid to the formation of E. coli biofilms. Unlike the presence of a derepressed F plasmid, the presence of a natural IncF F plasmid promoted biofilm formation by generating the cell-to-cell mating F pili between pairs of F+ cells (approximately two to four pili per cel

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16

Taylor, Diane E., and Elisa C. Brose. "Characterization of incompatibility group HI1 plasmids from Salmonella typhi by restriction endonuclease digestion and hybridization of DNA probes for Tn3, Tn9, and Tn10." Canadian Journal of Microbiology 31, no. 8 (1985): 721–29. http://dx.doi.org/10.1139/m85-136.

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Chloramphenicol resistance in Salmonella typhi is mediated by plasmids of the incompatibility group H, subgroup 1 (IncHI1). Eight IncHI1 plasmids from S. typhi strains originating in Mexico, Vietnam, Thailand, and India were examined by restriction enzyme digestion. The restriction enzymes, ApaI, XbaI, and PstI were found to be most useful for comparison of plasmid DNAs. Four plasmids from S. typhi isolated in Mexico, Vietnam, and Thailand between 1972 and 1974 had identical restriction patterns with all three enzymes. The other IncHI1 plasmids showed only minor differences. However, some sign

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17

Chu, Chishih, Cheng-Hsun Chiu, Chi-Hong Chu, and Jonathan T. Ou. "Nucleotide and Amino Acid Sequences of oriT-traM-traJ-traY-traA-traL Regions and Mobilization of Virulence Plasmids of Salmonella enterica Serovars Enteritidis, Gallinarum-Pullorum, and Typhimurium." Journal of Bacteriology 184, no. 11 (2002): 2857–62. http://dx.doi.org/10.1128/jb.184.11.2857-2862.2002.

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ABSTRACT The virulence plasmid of Salmonella enterica serovar Gallinarum-Pullorum (pSPV) but not those of Salmonella enterica serovars Enteritidis (pSEV) and Typhimurium (pSTV) can be readily mobilized by an F or F-like conjugative plasmid. To investigate the reason for the difference, the oriT-traM-traJ-traY-traA-traL regions of the three salmonella virulence plasmids (pSVs) were cloned and their nucleotide and deduced amino acid sequences were examined. The cloned fragments were generally mobilized more readily than the corresponding full-length pSVs, but the recombinant plasmid containing t

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García-Quintanilla, Meritxell, Ana I. Prieto, Laurent Barnes, Francisco Ramos-Morales, and Josep Casadesús. "Bile-Induced Curing of the Virulence Plasmid in Salmonella enterica Serovar Typhimurium." Journal of Bacteriology 188, no. 22 (2006): 7963–65. http://dx.doi.org/10.1128/jb.00995-06.

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ABSTRACT Exposure to bile induces curing of the virulence plasmid in Salmonella enterica serovar Typhimurium (pSLT). Disruption of the ccdB gene increases pSLT curing, both spontaneous and induced by bile, suggesting that the pSLT ccdAB genes may encode a homolog of the CcdAB addiction module previously described in the F sex factor. Unlike the F sex factor, synthesis of pSLT-encoded pili does not confer bile sensitivity. These observations may provide insights into the evolution of virulence plasmids in Salmonella subspecies I, as well as the causes of virulence plasmid loss in other Salmonel

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Boyd, E. Fidelma, and Daniel L. Hartl. "Salmonella Virulence Plasmid: Modular Acquisition of the spv Virulence Region by an F-Plasmid in Salmonella enterica Subspecies I and Insertion Into the Chromosome of Subspecies II, IIIa, IV and VII Isolates." Genetics 149, no. 3 (1998): 1183–90. http://dx.doi.org/10.1093/genetics/149.3.1183.

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Abstract The spv operon is common to all Salmonella virulence plasmids. DNA hybridization analysis indicates that the spv region is limited in distribution to serovars of Salmonella enterica subspecies I, II, IIIa, IV, and VII and is absent from Salmonella bongori isolates. Among strains of subspecies II, IIIa, and VII, all isolates examined contained sequences that hybridized with the spv region. However, among isolates of subspecies I, DNA sequences capable of hybridizing with the spv region were found in some isolates of certain serovars. Furthermore, in isolates of subspecies I, the virule

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Golubov, Andrey, Heinrich Neubauer, Christina Nölting, Jürgen Heesemann, and Alexander Rakin. "Structural Organization of the pFra Virulence-Associated Plasmid of Rhamnose-Positive Yersinia pestis." Infection and Immunity 72, no. 10 (2004): 5613–21. http://dx.doi.org/10.1128/iai.72.10.5613-5621.2004.

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ABSTRACT The 137,036-bp plasmid pG8786 from rhamnose-positive Yersinia pestis G8786 isolated from the high mountainous Caucasian plague focus in Georgia is an enlarged form of the pFra virulence-associated plasmid containing genes for synthesis of the antigen fraction 1 and phospholipase D. In addition to the completely conserved genes of the pFra backbone, pG8786 contains two large regions consisting of 4,642 and 32,617 bp, designated regions 1 and 2, respectively. Region 1 retains a larger part of Salmonella enterica serovar Typhi plasmid pHCM2 resembling the backbone of pFra replicons, whil

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Kline, Bruce C. "Aspects of plasmid F maintenance in Escherichia coli." Canadian Journal of Microbiology 34, no. 4 (1988): 526–35. http://dx.doi.org/10.1139/m88-090.

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A major class of replicons in procaryotes is typified by low copy number, nonrandom intracellular distribution, and stable inheritance. Included in this class are chromosomes of gram-positive and gram-negative bacteria as well as a number of plasmids from these organisms. Replicons in this major class have remarkable structural and functional similarities in the genes that effect and control replication. In the present work a review of plasmid F is presented as a paradigm for many aspects of this group's maintenance features.

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Slavcev, Roderick A., and Barbara E. Funnell. "Identification and Characterization of a Novel Allele of Escherichia coli dnaB Helicase That Compromises the Stability of Plasmid P1." Journal of Bacteriology 187, no. 4 (2005): 1227–37. http://dx.doi.org/10.1128/jb.187.4.1227-1237.2005.

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ABSTRACT Bacteriophage P1 lysogenizes Escherichia coli cells as a plasmid with approximately the same copy number as the copy number of the host chromosome. Faithful inheritance of the plasmids relies upon proper DNA replication, as well as a partition system that actively segregates plasmids to new daughter cells. We genetically screened for E. coli chromosomal mutations that influenced P1 stability and identified a novel temperature-sensitive allele of the dnaB helicase gene (dnaB277) that replaces serine 277 with a leucine residue (DnaB S277L). This allele conferred a severe temperature-sen

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Johnson, Timothy J., Kylie E. Siek, Sara J. Johnson, and Lisa K. Nolan. "DNA Sequence and Comparative Genomics of pAPEC-O2-R, an Avian Pathogenic Escherichia coli Transmissible R Plasmid." Antimicrobial Agents and Chemotherapy 49, no. 11 (2005): 4681–88. http://dx.doi.org/10.1128/aac.49.11.4681-4688.2005.

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ABSTRACT In this study, a 101-kb IncF plasmid from an avian pathogenic Escherichia coli (APEC) strain (APEC O2) was sequenced and analyzed, providing the first completed APEC plasmid sequence. This plasmid, pAPEC-O2-R, has functional transfer and antimicrobial resistance-encoding regions. The resistance-encoding region encodes resistance to eight groups of antimicrobial agents, including silver and other heavy metals, quaternary ammonium compounds, tetracycline, sulfonamides, aminoglycosides, trimethoprim, and beta-lactam antimicrobial agents. This region of the plasmid is unique among previou

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Sastre, J. Ignacio, Elena Cabezón, and Fernando de la Cruz. "The Carboxyl Terminus of Protein TraD Adds Specificity and Efficiency to F-Plasmid Conjugative Transfer." Journal of Bacteriology 180, no. 22 (1998): 6039–42. http://dx.doi.org/10.1128/jb.180.22.6039-6042.1998.

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ABSTRACT We isolated and characterized traD mutants with an altered specificity of interaction with relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) plasmids. The change in specificity was due to a loss of some amino acids in the carboxyl terminus of TraD that resulted in a broadening of the range of mobilizable relaxosomes at the expense of a decrease in the efficiency of F-plasmid transfer.

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Paula, Marcia O., Elerson GaettiI-Jardim Jr., and Mario J. Avilla-Campos. "Plasmid profile in oral Fusobacterium nucleatum from humans and Cebus apella monkeys." Revista do Instituto de Medicina Tropical de São Paulo 45, no. 1 (2003): 05–09. http://dx.doi.org/10.1590/s0036-46652003000100002.

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Fusobacterium nucleatum is a strict anaerobe and is indigenous of the human oral cavity. This organism is commonly recovered from different monomicrobial and mixed infections in humans and animals. In this study, the plasmid profile, the plasmid stability and the penicillin-resistance association in oral F. nucleatum isolated from periodontal patients, healthy subjects and Cebus apella monkeys were evaluated. Forty-five F. nucleatum strains from patients, 38 from healthy subjects and seven from C. apella were identified and analyzed. Plasmid extraction was performed in all the isolated strains

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Takai, Shinji, Masato Shoda, Yukako Sasaki, et al. "Restriction Fragment Length Polymorphisms of Virulence Plasmids in Rhodococcus equi." Journal of Clinical Microbiology 37, no. 10 (1999): 3417–20. http://dx.doi.org/10.1128/jcm.37.10.3417-3420.1999.

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Virulent Rhodococcus equi, which is a well-known cause of pyogranulomatous pneumonia in foals, possesses a large plasmid encoding virulence-associated 15- to 17-kDa antigens. Foal and soil isolates from five countries—Argentina, Australia, Canada, France, and Japan—were investigated for the presence of 15- to 17-kDa antigens by colony blotting, using the monoclonal antibody 10G5, and the gene coding for 15- to 17-kDa antigens by PCR. Plasmid DNAs extracted from positive isolates were digested with restriction endonucleasesBamHI, EcoRI, EcoT22I, andHindIII, and the digestion patterns that resul

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Bates, Steven, Annette M. Cashmore, and Brian M. Wilkins. "IncP Plasmids Are Unusually Effective in Mediating Conjugation of Escherichia coli and Saccharomyces cerevisiae: Involvement of the Tra2 Mating System." Journal of Bacteriology 180, no. 24 (1998): 6538–43. http://dx.doi.org/10.1128/jb.180.24.6538-6543.1998.

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ABSTRACT Mobilizable shuttle plasmids containing the origin-of-transfer (oriT) region of plasmids F (IncFI), ColIb-P9 (IncI1), and RP4/RP1 (IncPα) were constructed to test the ability of the cognate conjugation system to mediate gene transfer from Escherichia coli to Saccharomyces cerevisiae. Only the Pα system caused detectable mobilization to yeast, giving peak values of 5 × 10−5 transconjugants per recipient cell in 30 min. Transfer of the shuttle plasmid required carriage oforiT in cis and the provision intrans of the Pα Tra1 core and Tra2 core regions. Genes outside the Tra1 core did not

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Johnson, Timothy J., Sara J. Johnson, and Lisa K. Nolan. "Complete DNA Sequence of a ColBM Plasmid from Avian Pathogenic Escherichia coli Suggests that It Evolved from Closely Related ColV Virulence Plasmids." Journal of Bacteriology 188, no. 16 (2006): 5975–83. http://dx.doi.org/10.1128/jb.00204-06.

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ABSTRACT Avian pathogenic Escherichia coli (APEC), an extraintestinal pathogenic E. coli causing colibacillosis in birds, is responsible for significant economic losses for the poultry industry. Recently, we reported that the APEC pathotype was characterized by possession of a set of genes contained within a 94-kb cluster linked to a ColV plasmid, pAPEC-O2-ColV. These included sitABCD, genes of the aerobactin operon, hlyF, iss, genes of the salmochelin operon, and the 5′ end of cvaB of the ColV operon. However, the results of gene prevalence studies performed among APEC isolates revealed that

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Hu, Bo, Pratick Khara, and Peter J. Christie. "Structural bases for F plasmid conjugation and F pilus biogenesis inEscherichia coli." Proceedings of the National Academy of Sciences 116, no. 28 (2019): 14222–27. http://dx.doi.org/10.1073/pnas.1904428116.

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Bacterial conjugation systems are members of the large type IV secretion system (T4SS) superfamily. Conjugative transfer of F plasmids residing in theEnterobacteriaceaewas first reported in the 1940s, yet the architecture of F plasmid-encoded transfer channel and its physical relationship with the F pilus remain unknown. We visualized F-encoded structures in the native bacterial cell envelope by in situ cryoelectron tomography (CryoET). Remarkably, F plasmids encode four distinct structures, not just the translocation channel or channel-pilus complex predicted by prevailing models. The F1 stru

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Lazdins, Alessandro, Claire Miller, and Christopher M. Thomas. "Plasmids and the spread of antibiotic resistance." Biochemist 37, no. 3 (2015): 12–17. http://dx.doi.org/10.1042/bio03703012.

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The term plasmid was first coined in 1952 by Joshua Lederberg to encompass all extra-chromosomal hereditary determinants1. One of the first plasmids discovered allowed the movement of genes from one bacterial strain to another, creating a sort of ‘bacterial sex’ or ‘fertility’ so that the entity responsible was called the ‘fertility factor’ or F-factor2. Plasmids carrying antibiotic resistance were soon discovered and became a topic of intense research which contributed to the development of cloning vectors that underpinned the gene cloning revolution in the 1970s. Although the problems create

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Ho, Pak-Leung, Jane Chan, Wai-U. Lo, et al. "Prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes among blood and urinary Escherichia coli isolates." Journal of Medical Microbiology 62, no. 11 (2013): 1707–13. http://dx.doi.org/10.1099/jmm.0.062653-0.

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A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996–2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistance genes. Eighteen of the 1878 clinical E. coli isolates were fosfomycin resistant, of which six were fosA3 positive and two were positive for another fosA variant (designated fosKP96). No isolates had the fosC2 gene. The clones of the eight isolates were diverse: sequence type (ST

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Ravin, N., and D. Lane. "Partition of the Linear Plasmid N15: Interactions of N15 Partition Functions with the sop Locus of the F Plasmid." Journal of Bacteriology 181, no. 22 (1999): 6898–906. http://dx.doi.org/10.1128/jb.181.22.6898-6906.1999.

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ABSTRACT A locus close to one end of the linear N15 prophage closely resembles the sop operon which governs partition of the F plasmid; the promoter region contains similar operator sites, and the two putative gene products have extensive amino acid identity with the SopA and -B proteins of F. Our aim was to ascertain whether the N15sop homologue functions in partition, to identify the centromere site, and to examine possible interchangeability of function with the F Sop system. When expressed at a moderate level, N15 SopA and -B proteins partly stabilize mini-F which lacks its own sopoperon b

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Alvarez, B., P. Secades, M. J. McBride, and J. A. Guijarro. "Development of Genetic Techniques for the Psychrotrophic Fish Pathogen Flavobacterium psychrophilum." Applied and Environmental Microbiology 70, no. 1 (2004): 581–87. http://dx.doi.org/10.1128/aem.70.1.581-587.2004.

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ABSTRACT Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipulation of F. psychrophilum and the identification of plasmids, selectable markers, a reporter system, and a transposon that function in several isolates of this fish pathogen. The antibiotic resistance genes ermF, cfxA, and tetQ function in F. psychrophilum. Cloning vecto

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Kurittu, Paula, Banafsheh Khakipoor, Michael S. M. Brouwer, and Annamari Heikinheimo. "Plasmids conferring resistance to extended-spectrum beta-lactamases including a rare IncN+IncR multireplicon carrying blaCTX-M-1 in Escherichia coli recovered from migrating barnacle geese (Branta leucopsis)." Open Research Europe 1 (May 4, 2021): 46. http://dx.doi.org/10.12688/openreseurope.13529.1.

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Background: Increasing antimicrobial resistance (AMR) is a global threat and wild migratory birds may act as mediators of resistant bacteria across country borders. Our objective was to study extended-spectrum beta-lactamase (ESBL) and plasmid-encoded AmpC (pAmpC) producing Escherichia coli in barnacle geese using whole genome sequencing (WGS) and to identify plasmids harboring bla genes. Methods: Barnacle geese feces (n=200) were collected during fall 2017 and spring 2018 from an urban area in Helsinki, Finland. ESBL/AmpC-producing E. coli were recovered from nine samples (4.5%) and isolates

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Maier, Tamara M., Andrea Havig, Monika Casey, Francis E. Nano, Dara W. Frank, and Thomas C. Zahrt. "Construction and Characterization of a Highly Efficient Francisella Shuttle Plasmid." Applied and Environmental Microbiology 70, no. 12 (2004): 7511–19. http://dx.doi.org/10.1128/aem.70.12.7511-7519.2004.

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ABSTRACT Francisella tularensis is a facultative intracellular pathogen that infects a wide variety of mammals and causes tularemia in humans. It is recognized as a potential agent of bioterrorism due to its low infectious dose and multiple routes of transmission. To date, genetic manipulation in Francisella spp. has been limited due to the inefficiency of DNA transformation, the relative lack of useful selective markers, and the lack of stably replicating plasmids. Therefore, the goal of this study was to develop an enhanced shuttle plasmid that could be utilized for a variety of genetic proc

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Haft, Rembrandt J. F., Gilberto Palacios, Tran Nguyen, et al. "General Mutagenesis of F Plasmid TraI Reveals Its Role in Conjugative Regulation." Journal of Bacteriology 188, no. 17 (2006): 6346–53. http://dx.doi.org/10.1128/jb.00462-06.

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ABSTRACT Bacteria commonly exchange genetic information by the horizontal transfer of conjugative plasmids. In gram-negative conjugation, a relaxase enzyme is absolutely required to prepare plasmid DNA for transit into the recipient via a type IV secretion system. Here we report a mutagenesis of the F plasmid relaxase gene traI using in-frame, 31-codon insertions. Phenotypic analysis of our mutant library revealed that several mutant proteins are functional in conjugation, highlighting regions of TraI that can tolerate insertions of a moderate size. We also demonstrate that wild-type TraI, whe

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Venderbure, Claude, Arnaud Chastanet, François Boudsocq, Suzanne Sommer, and Adriana Bailone. "Inhibition of Homologous Recombination by the Plasmid MucA′B Complex." Journal of Bacteriology 181, no. 4 (1999): 1249–55. http://dx.doi.org/10.1128/jb.181.4.1249-1255.1999.

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ABSTRACT By its functional interaction with a RecA polymer, the mutagenic UmuD′C complex possesses an antirecombination activity. We show here that MucA′B, a functional homolog of the UmuD′C complex, inhibits homologous recombination as well. In F− recipients expressing MucA′B from a P tac promoter, Hfr × F−recombination decreased with increasing MucA′B concentrations down to 50-fold. In damage-induced pKM101-containing cells expressing MucA′B from the native promoter, recombination between a UV-damaged F lac plasmid and homologous chromosomal DNA decreased 10-fold. Overexpression of MucA′B to

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Yoshioka, Y., H. Ohtsubo, and E. Ohtsubo. "Repressor gene finO in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO." Journal of Bacteriology 169, no. 2 (1987): 619–23. http://dx.doi.org/10.1128/jb.169.2.619-623.1987.

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DuBow, Michael S., and Manon Lalumière. "The proximity of mini-Mu termini can affect the products of DNA transposition." Canadian Journal of Microbiology 40, no. 7 (1994): 567–75. http://dx.doi.org/10.1139/m94-091.

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Temperate bacteriophage Mu's 37 kilobase pair (kb) linear, double-stranded DNA requires DNA sequences at both termini in cis to transpose in a duplicative manner and thus propagate its genome during the lytic cycle. To define any spatial or orientation parameters between these sequences, we constructed "isomer" plasmids pMD186 and pMD861 containing identical Mu termini in close (or inverse) orientation 407 base pairs apart, or separated by 9.3 kb of plasmid pSC 101 DNA, respectively. Both mini-Mu plasmids transposed at equal frequencies onto an F′ episome in vivo, in the presence of an induced

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Bachrach, Gilad, Susan Kinder Haake, Alon Glick, et al. "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System." Applied and Environmental Microbiology 70, no. 12 (2004): 6957–62. http://dx.doi.org/10.1128/aem.70.12.6957-6962.2004.

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ABSTRACT Fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. Studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. Plasmids were isolated from eight strains of F. nucleatum. The smallest plasmid, pKH9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. DNA sequence analysis of pKH9 revealed an open reading frame (ORF) encoding a putative autonomous rolling circle replication protein

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Anthony, Karen G., William A. Klimke, Jan Manchak, and Laura S. Frost. "Comparison of Proteins Involved in Pilus Synthesis and Mating Pair Stabilization from the Related Plasmids F and R100-1: Insights into the Mechanism of Conjugation." Journal of Bacteriology 181, no. 17 (1999): 5149–59. http://dx.doi.org/10.1128/jb.181.17.5149-5159.1999.

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ABSTRACT F and R100-1 are closely related, derepressed, conjugative plasmids from the IncFI and IncFII incompatibility groups, respectively. Heteroduplex mapping and genetic analyses have revealed that the transfer regions are extremely similar between the two plasmids. Plasmid specificity can occur at the level of relaxosome formation, regulation, and surface exclusion between the two transfer systems. There are also differences in pilus serology, pilus-specific phage sensitivity, and requirements for OmpA and lipopolysaccharide components in the recipient cell. These phenotypic differences w

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Laurenzio, Laura, Laura S. Frost, and William Paranchych. "The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids." Molecular Microbiology 6, no. 20 (1992): 2951–59. http://dx.doi.org/10.1111/j.1365-2958.1992.tb01754.x.

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Sengupta, Manjistha, Henrik Jorck Nielsen, Brenda Youngren, and Stuart Austin. "P1 Plasmid Segregation: Accurate Redistribution by Dynamic Plasmid Pairing and Separation." Journal of Bacteriology 192, no. 5 (2009): 1175–83. http://dx.doi.org/10.1128/jb.01245-09.

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ABSTRACT Low-copy-number plasmids, such as P1 and F, encode a type Ia partition system (P1par or Fsop) for active segregation of copies to daughter cells. Typical descriptions show a single central plasmid focus dividing and the products moving to the cell quarter regions, ensuring segregation. However, using improved optical and analytical tools and large cell populations, we show that P1 plasmid foci are very broadly distributed. Moreover, under most growth conditions, more than two foci are frequently present. Each focus contains either one or two plasmid copies. Replication and focus split

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Neitz, M., and J. Carbon. "Characterization of a centromere-linked recombination hot spot in Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 11 (1987): 3871–79. http://dx.doi.org/10.1128/mcb.7.11.3871.

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A 1.5-kilobase-pair SalI-HindIII (SH) restriction fragment from the region of Saccharomyces cerevisiae chromosome XIV immediately adjacent to the centromere appears to contain sequences that act as a hot spot for mitotic recombination. The presence of SH DNA on an autonomously replicating plasmid stimulates homologous genetic exchange between yeast genomic sequences and those present on the plasmid. In all recombinants characterized, exchange occurs in plasmid yeast sequences adjacent to rather than within the SH DNA. Hybridization analyses reveal that SH-containing plasmids are present in lin

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Neitz, M., and J. Carbon. "Characterization of a centromere-linked recombination hot spot in Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 11 (1987): 3871–79. http://dx.doi.org/10.1128/mcb.7.11.3871-3879.1987.

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A 1.5-kilobase-pair SalI-HindIII (SH) restriction fragment from the region of Saccharomyces cerevisiae chromosome XIV immediately adjacent to the centromere appears to contain sequences that act as a hot spot for mitotic recombination. The presence of SH DNA on an autonomously replicating plasmid stimulates homologous genetic exchange between yeast genomic sequences and those present on the plasmid. In all recombinants characterized, exchange occurs in plasmid yeast sequences adjacent to rather than within the SH DNA. Hybridization analyses reveal that SH-containing plasmids are present in lin

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LIU, Y., L. G. WAN, Q. DENG, X. W. CAO, Y. YU, and Q. F. XU. "First description of NDM-1-, KPC-2-, VIM-2- and IMP-4-producing Klebsiella pneumoniae strains in a single Chinese teaching hospital." Epidemiology and Infection 143, no. 2 (2014): 376–84. http://dx.doi.org/10.1017/s0950268814000995.

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SUMMARYA total of 180 non-duplicate carbapenem-resistant Klebsiella pneumoniae isolates were recovered from patients hospitalized between December 2010 and January 2012 at a Chinese hospital. Eight KPC-2, four NDM-1, one VIM-2, and five KPC-2 plus IMP-4 producers were identified and all were multidrug resistant due to the presence of other resistance determinants, including extended-spectrum β-lactamases (CTX-M-15, SHV-12), 16S rRNA methylases (armA, rmtB) and plasmid-mediated quinolone-resistance determinants (qnrA, B, S, aac(6′)-Ib-cr). Nine K. pneumoniae clones (Kpn-A1/ST395, Kpn-A3/ST11, K

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Dostál, Lubomír, Sichen Shao, and Joel F. Schildbach. "Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer." Nucleic Acids Research 39, no. 7 (2010): 2658–70. http://dx.doi.org/10.1093/nar/gkq1137.

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Saavedra De Bast, Manuel, Natacha Mine, and Laurence Van Melderen. "Chromosomal Toxin-Antitoxin Systems May Act as Antiaddiction Modules." Journal of Bacteriology 190, no. 13 (2008): 4603–9. http://dx.doi.org/10.1128/jb.00357-08.

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ABSTRACT Toxin-antitoxin (TA) systems are widespread among bacterial chromosomes and mobile genetic elements. Although in plasmids TA systems have a clear role in their vertical inheritance by selectively killing plasmid-free daughter cells (postsegregational killing or addiction phenomenon), the physiological role of chromosomally encoded ones remains under debate. The assumption that chromosomally encoded TA systems are part of stress response networks and/or programmed cell death machinery has been called into question recently by the observation that none of the five canonical chromosomall

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El-Hage, Nazira, and Brian Stevenson. "Simultaneous Coexpression of Borrelia burgdorferi Erp Proteins Occurs through a Specific, erp Locus-Directed Regulatory Mechanism." Journal of Bacteriology 184, no. 16 (2002): 4536–43. http://dx.doi.org/10.1128/jb.184.16.4536-4543.2002.

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ABSTRACT An individual Borrelia burgdorferi bacterium can encode as many as 13 different Erp (OspE/F-related) proteins from mono-and bicistronic loci that are carried on up to 10 separate plasmids. We demonstrate through multilabel immunofluorescence analyses that individual bacteria simultaneously coexpress their entire Erp protein repertoire. While it has been proposed that B. burgdorferi controls expression of Erp and other plasmid-encoded proteins through changes in DNA topology, we observed regulated Erp expression in the absence of detectable differences in DNA supercoiling. Likewise, in

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Weber, Peter C., and Sunil Palchaudhuri. "Characterization of the stability functions and inverted repeat structure X3 of the IncFI plasmid ColV2-K94." Canadian Journal of Microbiology 32, no. 10 (1986): 806–13. http://dx.doi.org/10.1139/m86-148.

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A region of the IncFI plasmid ColV2-K94 which showed homology to the sop partitioning genes of F was cloned and characterized in an attempt to study the stability functions of this element. The sop region contained the incD incompatibility determinant common to many IncFI plasmids, but could not confer on ColV2-K94 miniplasmids the same stable inheritance found in the intact ColV2-K94; thus, other functions appear to be required for efficient plasmid maintenance. Adjacent to the area of sop homology was the X3 region, which was found to contain three inverted IS1-like sequences. The X3 region

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