Relevant bibliographies by topics / Plasmidic DNA

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Plasmidic DNA'

Author: Grafiati
Published: 9 March 2023
Last updated: 10 March 2023

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Journal articles on the topic "Plasmidic DNA"

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Wilbaux, Myriam, Natacha Mine, Anne-Marie Guérout, Didier Mazel, and Laurence Van Melderen. "Functional Interactions between Coexisting Toxin-Antitoxin Systems of the ccd Family in Escherichia coli O157:H7." Journal of Bacteriology 189, no. 7 (2007): 2712–19. http://dx.doi.org/10.1128/jb.01679-06.

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ABSTRACT Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd F) located in the chromosomal backbone of the pathogenic O157:H7 Escherichia coli strain (ccd O157). The ccd F and the ccd O157 systems coexist in O157:H7 isolates, as these pathogenic strains contain an F-related virulence plasmid carrying the ccd F system. We have shown that the chromosomal ccd O157 system encodes functional toxin and a
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Rivard, Sylvain R., Jean-Charles Côté, and Young Sup Chung. "Comparaison des profils plasmidiques de mutants oligosporogènes chez Bacillus thuringiensis var. kurstaki HD-1 après traitement à l'ozone et à la nitrosoguanidine." Genome 32, no. 4 (1989): 660–66. http://dx.doi.org/10.1139/g89-495.

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Two strains of Bacillus thuringiensis were used in order to isolate sporeless and crystalliferous mutants. These strains, B. thuringiensis var. kurstaki HD-1 and var. israelensis Q-2, produce a crystalline inclusion, δ-endotoxin, which is lethal for lepidopteran and dipteran larvae, respectively. Mutants derived from strain HD-1 were easily isolated because of their characteristic morphology. By comparing different plasmidic patterns of the strains, we can confirm the relationship existing between a DNA extrachromosomic element and the production of δ-endotoxin in B. thuringiensis. However, th
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Bauernfeind, A., I. Stemplinger, R. Jungwirth, S. Ernst, and J. M. Casellas. "Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases." Antimicrobial Agents and Chemotherapy 40, no. 2 (1996): 509–13. http://dx.doi.org/10.1128/aac.40.2.509.

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Amino acid sequences determined either by protein sequencing or by DNA sequencing are identical for cefotaximases CTX-M-1 and MEN-1, whereas CTX-M-2 is 84% identical to CTX-M-1/MEN-1. Both beta-lactamases are distantly related to other plasmidic class A enzymes (homology to TEM-1 is 38.1% for CTX-M-1/MEN-1 and 36.5% for CTX-M-2); the closest relationship was with the chromosomal beta-lactamase of Klebsiella oxytoca E23004 (homologies of 74.5% for CTX-M-1/MEN-1 and 77.9% for CTX-M-2). The cefotaximases CTX-M-1/MEN-1 and CTX-M-2 represent two members of a new subgroup of plasmidic class A beta-l
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Gonzalez, M. Micaela, Magali Pellon-Maison, Matias A. Ales-Gandolfo, Maria R. Gonzalez-Baró, Rosa Erra-Balsells та Franco M. Cabrerizo. "Photosensitized cleavage of plasmidic DNA by norharmane, a naturally occurring β-carboline". Organic & Biomolecular Chemistry 8, № 11 (2010): 2543. http://dx.doi.org/10.1039/c002235g.

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Conese, Massimo, Alessandra Biffi, Giorgia Dina, Nicola Marziliano, and Antonello Villa. "Comparison between Cationic Polymer and Lipid in Plasmidic DNA Delivery to the Cell Nucleus." Open Gene Therapy Journal 2, no. 1 (2009): 21–28. http://dx.doi.org/10.2174/1875037000902010021.

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García-Raso, A., J. J. Fiol, A. Tasada, et al. "Ruthenium complexes with purine derivatives: Syntheses, structural characterization and preliminary studies with plasmidic DNA." Inorganic Chemistry Communications 8, no. 9 (2005): 800–804. http://dx.doi.org/10.1016/j.inoche.2005.05.023.

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Llanes, Catherine, Veronique Kirchgesner та Patrick Plesiat. "Propagation of TEM- and PSE-Type β-Lactamases among Amoxicillin-Resistant Salmonella spp. Isolated in France". Antimicrobial Agents and Chemotherapy 43, № 10 (1999): 2430–36. http://dx.doi.org/10.1128/aac.43.10.2430.

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ABSTRACT A survey conducted between 1987 and 1994 at the University Hospital of Besançon, France, demonstrated a dramatic increase (from 0 to 42.5%) in the prevalence of amoxicillin resistance amongSalmonella spp. Of the 96 resistant isolates collected during this period (including 77 Typhimurium), 54 were found to produce TEM-1 β-lactamase, 40 produced PSE-1 (equivalent to CARB-2), one produced PSE-1 plus TEM-2, and one produced OXA-1 in isoelectric focusing and DNA hybridization experiments. Plasmids coding for these β-lactamases were further characterized by (i) profile analysis, (ii) rest
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Mollenkopf, Dixie F., Matthew F. Weeman, Joshua B. Daniels, et al. "Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle." Applied and Environmental Microbiology 78, no. 13 (2012): 4552–60. http://dx.doi.org/10.1128/aem.00373-12.

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ABSTRACTblaCTX-Mbeta-lactamases confer resistance to critically important cephalosporin drugs. Recovered from both hospital- and community-acquired infections,blaCTX-Mwas first reported in U.S. livestock in 2010. It has been hypothesized that veterinary use of cephalosporins in livestock populations may lead to the dissemination of beta-lactamase-encoding genes. Therefore, our objectives were to estimate the frequency and distribution of coliform bacteria harboringblaCTX-Min the fecal flora of Ohio dairy cattle populations. In addition, we characterized the CTX-M alleles carried by the isolate
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Fernández-de-Alba, Carles, Nicholas S. Berrow, Raquel Garcia-Castellanos, Jesús García, and Miquel Pons. "On the Origin of the Selectivity of Plasmidic H-NS towards Horizontally Acquired DNA: Linking H-NS Oligomerization and Cooperative DNA Binding." Journal of Molecular Biology 425, no. 13 (2013): 2347–58. http://dx.doi.org/10.1016/j.jmb.2013.03.006.

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Coton, Emmanuel, Niels Mulder, Monika Coton, Sylvie Pochet, Hein Trip, and Juke S. Lolkema. "Origin of the Putrescine-Producing Ability of the Coagulase-Negative Bacterium Staphylococcus epidermidis 2015B." Applied and Environmental Microbiology 76, no. 16 (2010): 5570–76. http://dx.doi.org/10.1128/aem.00441-10.

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ABSTRACT A multiplex PCR method, aimed at the detection of genes associated with biogenic amine production, identified the odc gene encoding ornithine decarboxylase in 1 of 15 strains of Staphylococcus epidermidis. The ability of the positive strain, S. epidermidis 2015B, to produce putrescine in vitro was demonstrated by high-performance liquid chromatography (HPLC). In this strain, the odc gene was detected on plasmid DNA, suggesting that the ability to form putrescine is carried by a mobile element, which explains the fact that the trait is strain dependent within the S. epidermidis species
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Dissertations / Theses on the topic "Plasmidic DNA"

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BORGO, FRANCESCA CLAUDIA. "Individuazione e tipizzazione polifasica di ecotipi funzionali di batteri lattici coinvolti nelle produzioni casearie italiane." Doctoral thesis, Università degli Studi di Milano, 2008. http://hdl.handle.net/2434/164585.

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This PhD thesis dealt with the phenotypic and genotypic characterization of LAB strains through a polyphasic taxonomic approach, molecular analyses such as PCR, ITS, ARDRA, the study of housekeeping genes, search and classification of plasmidic DNA. The integration of the phenotypic, biotechnological and genotypic results allowed the obtainment of fingerprinting specific at the strain-level and the possible discrimination of the strains in function of their ecological niches.
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Valchová, Michaela. "Biochemická analýza DNA interakčních partnerů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376818.

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This thesis was focused on DNA analysis. The fluorescently labelled oligonucleotides were at first hybridised and subsequently analysed by HRM analysis to determine the melting temperatures of the oligonucleotides depending on the environment. This thesis describes the change of melting temperature of oligonucleotides in environments containing mono and bivalent ions and the influence of protein binding on the stability of these DNA structures. From determined melting points, it was specified whether the ion/protein stabilised or destabilised the oligonucleotide. Furthermore, plasmids were iso
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Higuti, Eliza. "Estudo dos efeitos endócrinos e parácrinos do hormônio de crescimento após administração de DNA plasmidial em modelos animais de terapia gênica." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-05082011-105039/.

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Nosso grupo tem estudado uma estratégia alternativa de tratamento para a deficiência de hormônio de crescimento (DGH), utilizando a administração in vivo do plasmídeo pUC-UBI-hGH, seguida de eletroporação, no músculo quadríceps de camundongos anões e imunodeficientes (lit/scid), que proporcionou níveis sustentáveis de hGH na circulação durante 60 dias e um aumento significativo de peso dos animais tratados. Visando investigar os efeitos autócrinos/parácrinos e endócrinos derivados deste tipo de tratamento, e compará-los aos da administração diária por via parenteral do hormônio de crescimento
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Zainuddin, Zainul Fadziruddin. "Mycobacterial plasmids and related DNA sequences." Thesis, University of Surrey, 1988. http://epubs.surrey.ac.uk/843201/.

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An investigation into the presence of plasmids in Mycobacterium tuberculosis was conducted. No extrachromosomal DNA was found although some evidence for their presence were detected. Plasmid-related sequences were however found in the genomic DNA of M, tuberculosis. Isolation of plasmid-related sequences from a strain of M, tuberculosis resulted in two different DNA probes which showed high specificity for M, tuberculosis and related species. One of these probes gave banding patterns which suggest the presence of restriction fragment polymorphism in M, tuberculosis. The other probe gave bandin
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Forde, G. M. "Plasmid DNA purification by affinity methods." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599116.

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Two affinity mechanisms were investigated for their suitability to pDNA purification: (1) GSH/GST-ZnF/pTS: a dual affinity fusion protein, comprised of Glutathione-S-Transferase (GST) and a zinc finger transcription factor (ZnF), was utilised to purify target pDNA. The fusion protein was firstly adsorbed to an immobilised glutathione (GSH) ligand via the GST segment followed by specific adsorption of a pUC19 based plasmid (pTS) to the exposed zinc finger; and (2) lac I peptide/pLS3: a lac repressor (lacI) peptide that displays affinity for lac operator (lacO) sequences was used to purify targe
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Henderson, Dorian Travis. "Activity of the long and short forms of the plasmid-encoded primase, MobA and RepB', in vegetative and conjugal replication of the broad-host-rang plasmid R1162 /." Digital version accessible at:, 1998. http://wwwlib.umi.com/cr/utexas/main.

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Atuah, Kwame N. "Development of plasmid dna formulations for application as microspheric dna vaccines." Thesis, Aston University, 2001. http://publications.aston.ac.uk/10942/.

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This thesis describes an investigation into the fabrication of plasmid DNA, the active principle of DNA vaccines, into microspheres, based on the tenet of an increased cellular uptake of microparticulate matter by phagocytic cells. The formulation of plasmid DNA into microspheres using two methods, is presented. Formulation of microspheric plasmid DNA using the double emulsion solvent evaporation method and a spray-drying method was explored. The former approach involves formation of a double emulsion, by homogenisation. This method produced microspheres of uniform size and smooth morphology,
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Mearini, Giulia. "Sequence specific visualization of DNA in live mammalian cells." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974208795.

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Hobson, Philip Stanley. "Optimising the efficacy of plasmid DNA vaccines." Thesis, King's College London (University of London), 2004. https://kclpure.kcl.ac.uk/portal/en/theses/optimising-the-efficacy-of-plasmid-dna-vaccines(fd48c033-ff46-4c9b-b3cb-dc63c02c7824).html.

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Kim, Hong-Yeoul Carleton University Dissertation Biology. "Determinants of the E. coli polymerase I- independent and polymerase I- dependent pathways of replication of an incompatibility N group plasmid replicon." Ottawa, 1993.

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Books on the topic "Plasmidic DNA"

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Friedhelm, Meinhardt, and Klassen Roland, eds. Microbial linear plasmids. Berlin, 2007.

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William, Lowry, Morrey John, and Taped Technologies Production, eds. Plasmid DNA preparation [videorecording]. Taped Technology, 1990.

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M, Schleef, ed. Plasmids for therapy and vaccination. Wiley-VCH, 2001.

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Microbial megaplasmids. Springer, 2009.

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G, Lessie Thomas, and United States. Environmental Protection Agency, eds. Multiple replicons constituting the genome of pseudomonas cepacia 17616. Environmental Protection Agency], 1994.

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Ming, Marin. Induction of CTL responses by plasmid DNA immunization. National Library of Canada, 1999.

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Untersuchungen zur Expression und Funktion des linearen, mitochondrialen Plasmides pC1K1 von Claviceps purpurea. J. Cramer, 1992.

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Brown, Steven A. Screening for plasmid DNA in an exopolymer-producing psychrotrophic bacterium. Laurentian University, 1987.

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Langlois, Lizette. The curing of plasmid DNA from two isolates of Thiobacillus ferrooxidans. Laurentian University, 1992.

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Manocha, Marcus M. S. Isolation and characterization of genomic DNA sequences that enhance the stability of plasmid DNA in mammalian cells. Brock University, Centre for Biotechnology, 2005.

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Book chapters on the topic "Plasmidic DNA"

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Muntean, Cristina, A. Ioachim, and Calina Cornea. "Microwave Absorption in Plasmidic DNA Molecules." In Fifth International Conference on the Spectroscopy of Biological Molecules. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1934-4_22.

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Patrick Higgins, N., and Alexander V. Vologodskii. "Topological Behavior of Plasmid DNA." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch7.

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Tregoning, John S., and Ekaterina Kinnear. "Using Plasmids as DNA Vaccines for Infectious Diseases." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch33.

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Hallet, Bernard, Virginie Vanhooff, and François Cornet. "DNA Site-Specific Resolution Systems." In Plasmid Biology. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817732.ch7.

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Higgins, N. Patrick, and Alexander V. Vologodskii. "Topological Behavior of Plasmid DNA." In Plasmid Biology. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817732.ch8.

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Baek, Chang-Ho, Michael Liss, Kevin Clancy, Jonathan Chesnut, and Federico Katzen. "DNA Assembly Tools and Strategies for the Generation of Plasmids." In Plasmids. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818982.ch30.

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Schleef, Martin, Torsten Schmidt, Karl Friehs, and Erwin Flaschel. "Plasmid DNA Manufacturing." In Manufacturing of Gene Therapeutics. Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-1353-7_8.

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Boffey, Stephen A. "Isolation of Plasmid DNA." In Springer Protocols Handbooks. Humana Press, 1986. http://dx.doi.org/10.1007/978-1-60327-405-0_2.

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Surzycki, Stefan. "Isolation of Plasmid DNA." In Basic Techniques in Molecular Biology. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-56968-5_5.

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Escoffre, Jean-Michel, Marie-Pierre Rols, and David A. Dean. "Electrotransfer of Plasmid DNA." In Clinical Aspects of Electroporation. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-8363-3_13.

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Conference papers on the topic "Plasmidic DNA"

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Yuan, Fan, David Zaharoff, Xiao-Yu Zhang, Frank Lohr, Mark W. Dewhirst, and Chuan-Yuan Li. "Delivery of Plasmid DNA Through Intratumoral Infusion and Electroporation." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2571.

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Abstract We investigated DNA transport in the interstitial space and across cell membrane facilitated by intratumoral infusion and in vivo electroporation, respectively. In the study, a rat fibrosarcoma was perfused ex vivo, and apparent hydraulic conductivity (Kapp) was quantified under different perfusion conditions. In addition, three plasmid DNA vectors were infused into solid tumors. Immediately after infusion, tumors were treated with or without electric pulses. Gene expression and tumor growth delay were determined at different time points after electroporation. We found that Kapp was v
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Yuan, Fan, David Zaharoff, Xiao-Yu Zhang, Frank Lohr, Mark W. Dewhirst, and Chuan-Yuan Li. "Delivery of Plasmid DNA Through Intratumoral Infusion and Electroporation." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2231.

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Abstract We investigated DNA transport in the interstitial space and across cell membrane facilitated by intratumoral infusion and in vivo electroporation, respectively. In the study, a rat fibrosarcoma was perfused ex vivo, and apparent hydraulic conductivity (Kapp) was quantified under different perfusion conditions. In addition, three plasmid DNA vectors were infused into solid tumors. Immediately after infusion, tumors were treated with or without electric pulses. Gene expression and tumor growth delay were determined at different time points after electroporation. We found that Kapp was v
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James, Molly B., and Todd D. Giorgio. "Nuclear-Associated Plasmid, But Not Cell-Associated Plasmid, is Correlated With Transgene Expression in Cultured Mammalian Cells." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2232.

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Abstract Intracellular plasmid is rapidly incorporated into the nucleus of HeLa cells following cationic lipoplex transfection. CV1 cells are less effective in translocating plasmid to the nucleus and also express less transgene than HeLa cells. Cultured HeLa and CV1 cells and corresponding isolated nuclei were analyzed after transfection of a Cy3 labeled pGreenLantern plasmid (Cy3-pGL). Flow cytometry was used to measure both plasmid delivery and transgene expression from the plasmid encoding a CMV promoter driven green fluorescent protein. During transfection, HeLa cells rapidly incorporated
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Yin, zhixiang, Hua chen, and Song Bosheng. "Plasmid DNA computing model of 0–1 programming problem." In 2010 IEEE Fifth International Conference on Bio-Inspired Computing: Theories and Applications (BIC-TA). IEEE, 2010. http://dx.doi.org/10.1109/bicta.2010.5645341.

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Subhi, Hanan, Fatimah Abdel, and Ihsan Raheem. "Isolation of plasmid DNA from Antibiotics Resistant Staphylococcus aureus." In 2018 International Conference on Pure and Applied Science. Koya University, 2018. http://dx.doi.org/10.14500/icpas2018.mim105.

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Oshin, Edwin, Chunqi Jiang, Richard Heller, and Pavan Cherukuri. "Evaluation of Plasma-Activated Plasmid DNA Delivery to Skin." In 2021 IEEE International Conference on Plasma Science (ICOPS). IEEE, 2021. http://dx.doi.org/10.1109/icops36761.2021.9588608.

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Li, Ming, Tao Niu, Jun-huan Zhang, Zhe Su, and Dong-jun Kong. "High Electroporation Efficiency of Corynebacterium glutamicum with Xenogeneic Plasmid DNA." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5517786.

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O'Connell, Deborah, Laura Cox, Wendy Hyland, et al. "Interactions of cold atmospheric pressure plasma jets with plasmid DNA." In 2010 IEEE 37th International Conference on Plasma Sciences (ICOPS). IEEE, 2010. http://dx.doi.org/10.1109/plasma.2010.5534053.

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Greenland, John, Thomas Rodgers, and Norman Letvin. "Lung-targeted Plasmid DNA Vaccines Induce Potent Cellular Immune Responses." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a3782.

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Wang, Yang, Bill McBride, David M. Goldenberg, and Chien-Hsing Chang. "Abstract 4445: Novel antibody-dendrimer conjugates efficiently complex plasmid DNA." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-4445.

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Reports on the topic "Plasmidic DNA"

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Peak, J. G., T. Ito, M. J. Peak, and F. T. Robb. DNA damage produced by exposure of supercoiled plasmid DNA to high- and low-LET ionizing radiation: Effects of hydroxyl radical quenchers. DNA breakage, neutrons, OH radicals. Office of Scientific and Technical Information (OSTI), 1994. http://dx.doi.org/10.2172/10172487.

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Kralj, Jason, Stephanie Servetas, Monique Hunter, et al. Certification of Standard Reference Material® 2917: Plasmid DNA for Fecal Indicator Detection and Identification. National Institute of Standards and Technology, 2021. http://dx.doi.org/10.6028/nist.sp.260-221.

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Weil, Clifford F., Anne B. Britt, and Avraham Levy. Nonhomologous DNA End-Joining in Plants: Genes and Mechanisms. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585194.bard.

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Repair of DNA breaks is an essential function in plant cells as well as a crucial step in addition of modified DNA to plant cells. In addition, our inability to introduce modified DNA to its appropriate locus in the plant genome remains an important hurdle in genetically engineering crop species.We have taken a combined forward and reverse genetics approach to examining DNA double strand break repair in plants, focusing primarily on nonhomologous DNA end-joining. The forward approach utilizes a gamma-plantlet assay (miniature plants that are metabolically active but do not undergo cell divisio
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DeMartini, James C., Abraham Yaniv, Jonathan O. Carlson, et al. Evaluation of Naked Proviral DNA as a Vaccine for Ovine Lentivirus Infection. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570553.bard.

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Ovine lentivirus (OvLV) infection is widespread in sheep of the United States and Israel and is responsible for substantial economic losses. The primary goal of this project was to evaluate naked proviral DNA as a vaccine to induce protective immunity in sheep in endemic areas. Contrary to expectations, inoculation of sheep with proviral DNA derived from the full length OvLV molecular clone pkv72 did not result in detectable OvLV infection, but infectious virus was recovered from transfected ovine cells. Kv72 virus produced by these cells infected sheep and induced antibody responses, and was
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Tzfira, Tzvi, Michael Elbaum, and Sharon Wolf. DNA transfer by Agrobacterium: a cooperative interaction of ssDNA, virulence proteins, and plant host factors. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7695881.bard.

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Agrobacteriumtumefaciensmediates genetic transformation of plants. The possibility of exchanging the natural genes for other DNA has led to Agrobacterium’s emergence as the primary vector for genetic modification of plants. The similarity among eukaryotic mechanisms of nuclear import also suggests use of its active elements as media for non-viral genetic therapy in animals. These considerations motivate the present study of the process that carries DNA of bacterial origin into the host nucleus. The infective pathway of Agrobacterium involves excision of a single-stranded DNA molecule (T-strand
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Barefoot, Susan, Benjamin Juven, Thomas Hughes, et al. Characterization of Bacteriocins Produced by Food Bioprocessing Propionobacteria. United States Department of Agriculture, 1992. http://dx.doi.org/10.32747/1992.7561061.bard.

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Objectives were to further characterize activity spectra of dairy propionibacteria bacteriocins, jenseniin G and propionicin PLG-1, purify them, examine the role of cell walls in resistance, examine their interactions with cytoplasmic membrane, explain producer immunity, and clone the responsible genes. Inhibitory spectra of both bacteriocins were further characterized. Propionicin was most effective in controlling Gram-positive, rather than Gram-negative organisms; it controlled growth of sensitive cells both in a culture medium and a model food system. Jenseniin inhibited yogurt cultures and
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Bostock, Richard M., Dov Prusky, and Martin Dickman. Redox Climate in Quiescence and Pathogenicity of Postharvest Fungal Pathogens. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586466.bard.

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Monilinia fructicola causes brown rot blossom blight and fruit rot in stone fruits. Immature fruit are highly resistant to brown rot but can become infected. These infections typically remain superficial and quiescent until they become active upon maturation of the fruit. High levels of chlorogenic acid (CGA) and related compounds occur in the peel of immature fruit but these levels decline during ripening. CGA inhibits cutinase expression, a putative virulence factor, with little or no effect on spore germination or hyphal growth. To better understand the regulation of cutinase expression by
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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate
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Freeman, Stanley, and Russell J. Rodriguez. The Interaction Between Nonpathogenic Mutants of Colletotrichum and Fusarium, and the Plant Host Defense System. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573069.bard.

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The intent of this proposal was to study the interaction between nonpathogenic mutants of Colletotrichum magna and Fusarium oxysporum, and the cucurbit host defense system. We had shown previously that a nonpathogenic endophytic mutant path- 1 of C. magna, caused no visible disease symptoms but protected watermelon seedlings from disease caused by the wildtype isolate and F. o. niveum. Objectives were: 1) Determine the microscopic, biochemical and molecular genetic interaction between "protected" (path- 1 colonized) cucurbit hosts and wildtype isolates of C. magna; 2) Isolate non-pathogenic mu
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Droby, Samir, Joseph W. Eckert, Shulamit Manulis, and Rajesh K. Mehra. Ecology, Population Dynamics and Genetic Diversity of Epiphytic Yeast Antagonists of Postharvest Diseases of Fruits. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568777.bard.

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One of the emerging technologies is the use of microbial agents for the control of postharvest diseases of fruits and vegetables. A number of antagonistic microorganisms have been discovered which have the potential to effectively control postharvest diseases. Some of this technology has been patented and commercial products such as AspireTM (Ecogen Corporatin, Langhorne, PA, USA), Biosave 10TM and Biosave 11TM (Ecoscience Inc., Worchester, MA, USA) have been registered for commercial use. The principal investigator of this project was involved in developing the yeast-based biofungicide-Aspire
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