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1

Silveira, Érico Leandro da [UNESP]. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA." Universidade Estadual Paulista (UNESP), 2004. http://hdl.handle.net/11449/94868.

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Made available in DSpace on 2014-06-11T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-04-07Bitstream added on 2014-06-13T19:14:40Z : No. of bitstreams: 1 silveira_el_me_jabo.pdf: 655008 bytes, checksum: ac46de019a2703894b3c1bc37633b43c (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da con
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Silveira, Érico Leandro da. "Identificação de comunidades bacterianas de solo por seqüenciamento do gene 16S rRNA /." Jaboticabal : [s.n.], 2004. http://hdl.handle.net/11449/94868.

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Resumo: Métodos tradicionais de isolamento e cultivo limitam análises da diversidade microbiana no meio ambiente, pois acredita - se que aproximadamente 10% desses microrganismos possam ser cultivados. A ecologia microbiana molecular teve recentes progressos através da construção de bibliotecas metagenômicas, o que constitui uma poderosa abordagem para explorar a diversidade microbiana de solo fornecendo inclusive dados sobre os microrganismos não cultiváveis desse habitat. Este trabalho teve por objetivo comparar e estimar a diversidade de comunidades bacterianas, em solos de duas áreas, send
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3

Pereira, Juliana Vianna. "Comparação por análise molecular da diversidade bacteriana da saliva de pacientes com diferentes índices de higiene bucal." Universidade Federal do Amazonas, 2009. http://tede.ufam.edu.br/handle/tede/3064.

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Made available in DSpace on 2015-04-20T12:31:09Z (GMT). No. of bitstreams: 1 Juliana Vianna Pereira.pdf: 4570056 bytes, checksum: c660fca0452deacfa10d23ee9bc79a2b (MD5) Previous issue date: 2009-08-26<br>The complex microbiota of the oral cavity has been intensively studied and saliva is characterized by microorganisms which colonize different regions of mouth, such as tongue, supragengival and subgingival biofilm. Considering this, the purpose of this study was to evaluate the bacterial diversity of the saliva of patients with different levels of oral hygiene according to the Silness,
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4

Grešová, Katarína. "Klasifikace bakterií do taxonomických kategorií na základě vlastností 16s rRNA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2020. http://www.nusl.cz/ntk/nusl-417246.

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The main goal of this thesis was to design and implement a tool that would be able to classify the sequences of the 16S rRNA gene into taxonomic categories using the properties of the 16S rRNA gene. The created tool analyzes all input sequences simultaneously, which differs from common classification approaches, which classify input sequences individually. This tool relies on the fact that bacteria contain several copies of the 16S rRNA gene, which may differ in sequence. The main contribution of this work is design, implementation and evaluation of the capabilities of this tool. Experiments h
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Kim, Min Seok. "AN INTEGRATED INVESTIGATION OF RUMINAL MICROBIAL COMMUNITIES USING 16S rRNA GENE-BASED TECHNIQUES." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316383870.

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Oliveira, Rosangela Claret de. "Isolamento de ureaplasma e micoplasma do trato reprodutivo de ovinos e caprinos e tipificação genotípica por meio da PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-12012009-105144/.

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Micoplasmas e ureaplasmas têm sido isolados de pequenos ruminantes, mas existem poucos estudos. O Mycoplasma ovine/caprine sorotipo 11, conhecido como cepa 2D, ainda não classificado como espécie, vem sendo relacionado a casos de vulvovaginite e problemas reprodutivos em ovinos e caprinos. Os ureaplasmas apresentam a habilidade em induzirem doenças no trato genital, confirmada por inoculações experimentais de isolados obtidos de animais doentes em animais saudáveis, resultando em moderada granularidade e hiperemia na vulva. Estes ureaplasmas não receberam ainda a designação de espécie, sendo a
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De, Moors Anick. "Comparison of gene organization in the region that surrounds the 16S rRNA gene in seven different Sulfolobales." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32533.pdf.

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8

Thorson, Mary Leah. "Characterization of the structure and function of a Bacteroides thetaiotaomicron 16S rRNA promoter." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/42962.

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The bacteroides group is a subdivision in the <I>Cytophaga-Flavobacterium-Bacteroides</I> phylum. This group is as phylogenetically distinct from other Gram-negative enterics, including <I>Escherichia coli</I>, as they are from Gram-positive organisms. Furthermore, there is no cross expression between genes of <I>E. coli</I> and <I>Bacteroides</I> species. It is thought that this difference in gene expression lies in part at the level of transcription initiation and is due to the sequences within the promoter region itself. A putative consensus sequence for <I>Bacteroides</I> promoters has
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9

Kam, Sin-yee. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971052.

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Aiemsum-ang, Porntipa. "Isolation, Systematics and Screening of Members of the Streptomyces violaceusniger 16S rRNA Gene Clade." Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506611.

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11

金倩儀 and Sin-yee Kam. "Application of 16S rRNA gene sequencing in laboratory diagnosis of mycobacteria other than tuberculosis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971052.

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Duangmal, Kannika. "Systematics and comparative genomics of members of the Streptomyces violaceoruber 16S rRNA gene clade." Thesis, University of Newcastle Upon Tyne, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403656.

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Lanyon, Clare. "16S rRNA gene sequence analysis of non-methanogenic Archaea in a hypereutrophic freshwater lake." Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402322.

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Hermansson, Anna. "Ammonia-oxidising bacteria in soil : studies of diversity and abundance using 16S rRNA gene analysis /." Linköping : Univ, 2001. http://www.bibl.liu.se/liupubl/disp/disp2001/tek712s.pdf.

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Del, Valle Luis J., Michael L. Jaramillo, Miguel Talledo, et al. "Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections." Horizon Research Publishing, 2014. http://hdl.handle.net/10757/322342.

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Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic
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Ng, Ho-yin Ricky, and 吳浩然. "Identification of anaerobic, non-sporulating, Gram-positive bacilli from blood cultures by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44670424.

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17

Kumar, Yashawant. "Mapping metabolites to the phylogeny of strains belonging to the 'Streptomyces violaceusniger' 16S rRNA gene clade." Thesis, University of Newcastle Upon Tyne, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442218.

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18

Patuzzi, Ilaria. "16S rRNA gene sequencing sparse count matrices: a count data simulator and optimal pre-processing pipelines." Doctoral thesis, Università degli studi di Padova, 2018. http://hdl.handle.net/11577/3426369.

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The study of microbial communities has deeply changed since it was firstly introduced in the 17th century. In the late 1970s, a breakthrough in the way bacterial communities were studied was brought by the discovery that ribosomal RNA (rRNA) genes could be used as molecular markers to perform organisms classification. Some decades later, the advent of DNA sequencing technology revolutionized the study of microbial communities, permitting a culture-independent view on the overall community contained within a sample. Today, one of the most widely used approaches for microbial communities profili
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Al, Masalma Mouhamad. "Molecular and cultural analysis of the bacterial flora associated with brain abscesses." Thesis, Aix-Marseille 2, 2011. http://www.theses.fr/2011AIX20663/document.

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Les abcès cérébraux sont des infections potentiellement mortelles, entraînant souvent des séquelles graves. La prise en charge médicale en reste empirique en raison d’un manque de connaissance approfondie des microorganismes responsables de cette condition. Dans la plupart des laboratoires microbiologiques, le diagnostic d’abcès cérébral est basé sur la culture du pus recueilli chirurgicalement. Malheureusement, cette procédure a de nombreuses limites et ne permet l’identification que d’une petite partie de la population microbienne en cause. L’amplification par PCR et le séquençage du gène co
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Fisher, Marc Lewis. "Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/27379.

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Termites are known to harbor within their gut a diverse assemblage of symbiotic microorganisms. Little work has been done, however, to describe the diversity and function of the bacteria in the economically important eastern subterranean termite, Reticulitermes flavipes. The first object of this study was to characterize the bacterial diversity in the gut of R. flavipes using amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyz
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Terceti, Mateus de Souza. "Diversidade bacteriana do gene 16S rRNA em carvão pirogênico de Terra Preta Antropogênica da Amazônia Central e Oriental." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-18122009-143646/.

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A Terra Preta Antropogênica (TPA) tem essa denominação porque é encontrada em sítios arqueológicos, onde viveram grupos pré-históricos e é considerada um dos solos mais férteis do mundo. Nela é encontrada grande quantidade de material deixado por grupos indígenas como fragmentos cerâmicos, artefatos líticos, e especialmente carvão pirogênico. Estudos realizados com o carvão pirogênico verificaram que ele aumenta a capacidade de trocas catiônicas nesses solos. Por meio de microscopia de fluorencência, foi observada a presença de microrganismos habitando esse carvão, no entanto, não se sabe quai
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Danckert, Nathan Peter. "Characterising the intestinal microbiome of Australian aquacultured abalone." Thesis, The University of Sydney, 2019. https://hdl.handle.net/2123/21643.

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Abalone are marine molluscs that are highly prized seafood delicacies. Australia produces more than 1,000 metric tonnes of abalone from aquaculture. Abalone aquaculture research priorities are to improve the growth rates and reduce the mortalities of abalone in aquaculture. Currently, little is known about the microbial communities associated with Australian abalone aquaculture; however, pathogens are associated with high mortality rates in the summer months. By utilising the 16S rRNA gene and high-throughput sequencing, the broad aim of this thesis was to characterise the microbiomes of Austr
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Baeza, Laurence. "Identification, caractérisation et purification des protéines chloroplastiques affines pour le promoteur du gène de l'ARNr 16S plastidial de l'épinard." Grenoble 1, 1993. http://www.theses.fr/1993GRE10204.

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Thi, Tuyen Do, Quyen Le Dinh, Thi Quyen Dinh, and Cuong Pham Van. "Isolation and identification of marine bacteria from marine mud in Vietnam with antimicrobial activity." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-99508.

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Seventeen bacterial strains were isolated from 9 marine mud samples from the inshore environments of the East Sea. Four bacterial strains showed an inhibition against all tested microorganisms Staphylococcus aureus ATCC10832, Escherichia coli JM109, and Fusarium oxysporum. 16S rRNA sequences of four bacterial strains were obtained by PCR using specific primers. PCR products were cloned into E. coli DH5a using pJET1.2 blunt vector. The recombinant plasmids were sequenced and the lengths of these 16S rRNA sequences were ~930bp. The 16S rRNA sequence from the four bacterial DB1.2, DB1.2.3, DB4.2
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Pereira, Thays Benites Camargo. "Identificação de diversidade genética em fitoplasmas com base na análise molecular dos gene 16S rRNA, SecY e Proteína Ribossomal." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-07012016-175356/.

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Os fitoplasmas são organismos procariotos sem parede celular, que habitam as células do floema das plantas e são transmitidos, principalmente, por cigarrinhas sugadoras de floema. Este patógeno é responsável por causar doenças em diversas espécies vegetais, provocando a expressão de diversos sintomas, entre os quais podem ser destacados a redução do tamanho foliar, amarelecimento das folhas, superbrotamento de ramos e enfezamento da planta hospedeira. Entre os anos de 2013 e 2014, plantas de três espécies vegetais com sintomas característicos de infecção causada por fitoplasmas foram observada
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PAMPLONA, NETO Christóvam. "Filogenia molecular dos xenarthra (Mammalia): análise do grupo cingulata a partir de sequências nucleotídicas do gene mitocondrial rRNA 16S." Universidade Federal do Pará, 2007. http://repositorio.ufpa.br/jspui/handle/2011/4232.

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Compte, Port Sergi. "Factors affecting the distribution, abundance and diversity of uncultured archaeal groups in freshwater sediments." Doctoral thesis, Universitat de Girona, 2018. http://hdl.handle.net/10803/663927.

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Archaea are abundant in extreme environments but they are also a prevalent component of microbial communities in soils, marine or freshwater plankton and sediments. Many studies highlighted the prominent role of marine sedimentary Archaea in global nutrient cycles. Less information is available, however, on the diversity, abundance and ecological role of Archaea in freshwater sediments. A serious pitfall for the study of Archaea is their low cultivability under laboratory-controlled conditions thus constraining the progress towards the complete understanding of their metabolic capabilities and
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Souza, Roberto Antonio de. "Identificação de linhagens atípicas de Yersinia spp. por métodos moleculares." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-26052009-161112/.

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O gênero Yersinia compreende 12 espécies. Y. enterocolitica, Y. pseudotuberculosis e Y. pestis são patógenos de vários animais, incluindo os humanos. Y. aldovae, Y. bercovieri, Y. frederiksenii, Y. intermedia, Y. kristensenii, Y. aleksiciae, Y. mollaretti e Y. rhodei são encontradas sobretudo no meio ambiente e alimentos e consideradas, usualmente, como bactérias oportunistas não-patogênicas e Y. ruckeri é um importante patógeno de peixes. Usualmente, as linhagens de Yersinia são classificadas em espécies de acordo com suas características bioquímicas. O Laboratório Nacional de Referência em Y
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Costa, Poliene Martins. "Caracterização da comunidade bacteriana associada ao trato intestinal de Spodoptera frugiperda provenientes de diferentes dietas." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-26042016-104030/.

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A importância da praga Spodoptera frugiperda (Lepidoptera: Noctuidae) deve-se não somente aos danos provocados às lavouras de milho, mas a capacidade de se alimentar de uma ampla variedade de famílias de plantas. Lagartas desta espécie são capazes de se adaptar a dietas contendo inibidores de peptidase de soja (IPS). Há uma hipótese de que a microbiota intestinal neste inseto poderia estar envolvida com estes mecanismos de adaptação. Neste contexto, um dos objetivos do trabalho foi verificar se estas bactérias poderiam alterar a expressão gênica de serino peptidases e atividade enzimática nest
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Dobbler, Priscila Caroline Thiago. "Baixa diversidade e sucessão microbiana anormal estão associadas à enterocolite necrosante em recém-nascidos prematuros." Universidade Federal do Pampa, 2017. http://dspace.unipampa.edu.br:8080/xmlui/handle/riu/1608.

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Submitted by Ana Damasceno (ana.damasceno@unipampa.edu.br) on 2017-06-07T18:12:48Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Baixa diversidade e sucessão microbiana anormal estão associadas à enterocolite necrosante em recém-nascidos prematuros.pdf: 1587508 bytes, checksum: c407e4cf94f25b2272a7d25213f72873 (MD5)<br>Made available in DSpace on 2017-06-07T18:12:48Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Baixa diversidade e sucessão microbiana anormal estão associadas à enteroc
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Calus, Szymon Tomasz. "Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex microbial communities : method development for accurate 16S rRNA gene amplicon sequencing." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/41086/.

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Nucleic acid sequencing can provide a detailed overview of microbial communities in comparison with standard plate-culture methods. Expansion of high-throughput sequencing (HTS) technologies and reduction in analysis costs has allowed for detailed exploration of various habitats with use of amplicon, metagenomics, and metatranscriptomics approaches. However, due to a capital cost of HTS platforms and requirements for batch analysis, genomics-based studies are still not being used as a standard method for the comprehensive examination of environmental or clinical samples for microbial character
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Yeung, Shiu-yan, and 楊兆恩. "Update and evaluation of 16SpathDB, an automated comprehensive database for identification of medically important bacteria by 16S rRNA gene sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193552.

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Identification of pathogens is one of the important duties of clinical microbiology laboratory. Traditionally, phenotypic tests are used to identify the bacteria. However, due to some limitations of the phenotypic tests, the bacteria may not be identified sometimes and cannot be identified promptly. 16S rRNA gene sequencing is a rapid and accurate method to achieve this target. It is especially useful for identify rare or slow growing bacteria. However, the interpretation of the 16S rRNA gene sequencing result is one of the challenging duties to laboratory technicians and microbiologists. Apar
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Yamaguti, Mauricio. "Isolamento de micoplasma de suínos com problemas respiratórios e tipificação dos isolados pela PFGE e seqüenciamento do gene 16S rRNA." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-29102009-091226/.

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As doenças respiratórias são responsáveis, na suinocultura, por grandes perdas econômicas e entre os agentes destacam-se os micoplasmas. Foram coletadas 126 amostras de muco nasal/tonsilar, e fragmentos de 78 pulmões, 2 de traquéia e 2 de tonsila. No isolamento foi utilizado o meio FRIIS modificado, na identificação, a Multiplex-PCR e na tipificação, a PFGE e sequenciamento do gene 16S rRNA. Foram obtidos 59 isolados identificados como M. hyopneumoniae (1,70%), M. flocculare (3,40%) e M. hyorhinis (94,90%). A PFGE dos isolados de M. hyorhinis, resultou em 10 e 9 perfis com a enzima AvaI e XhoI
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Mestrinelli, Fabiana. "Isolamento e caracterização de microrganismo envolvido na desnitrificação autrófica pela oxidação de sulfeto em reator vertical de leito fixo." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-19112010-150533/.

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O presente estudo teve como objetivo avaliar a comunidade envolvida na desnitrificação autotrófica pela oxidação de sulfetos, aplicada ao pós-tratamento de efluentes anaeróbios. O enriquecimento da comunidade bacteriana e da comunidade desnitrificante autotrófica foi realizado a partir de amostras da biomassa coletadas de três reatores verticais de leito fixo operados em condições distintas, sendo, redução autotrófica de nitrato, redução autotrófica de nitrito e redução autotrófica de nitrato com excesso de sulfeto. Após a determinação da melhor condição de enriquecimento, a cultura foi purifi
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Rezende, Lilian Ribeiro. "Caracterização molecular da comunidade bacteriana em rebanhos leiteiros com mastite subclínica." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-10082016-130206/.

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A mastite bovina é considerada a doença de maior impacto nos rebanhos leiteiros, exercendo efeito econômico negativo sobre a produtividade e perdas significativas à indústria de laticínios. Tendo em vista os impactos na sanidade animal e os prejuízos econômicos acarretados, o objetivo deste estudo foi caracterizar de forma mais abrangente a comunidade microbiana presente em rebanhos leiteiros com mastite subclínica utilizado o sequenciamento parcial do gene 16S ribossomal RNA (rRNA). Especificamente, foram caracterizadas as comunidades bacterianas presentes em amostras de leite vindas de três
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Mulheron, Rebecca. "Microbial Community Assembly found with Sponge Orange Band Disease in Xestospongia muta (Giant Barrel Sponge)." NSUWorks, 2014. http://nsuworks.nova.edu/occ_stuetd/18.

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The giant barrel sponge, Xestospongia muta is an iconic and essential species of the coral reefs in South Florida. The sponge has primary roles providing ecosystem services and creating unique habitats for diverse microbial communities. On April 27, 2012 an outbreak of Sponge Orange Band Disease (SOB) was detected off the coast of South Florida. The disease begins with sponge bleaching, followed by mesohyl or “mesohyl” necrosis and often total mesohyl disintegration. Sampling from two diseased populations at Boynton Beach and Fort Lauderdale, FL took place on May 11th and May 29th, 2012. Each
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Erram, Dinesh. "Characterization of the larval habitat of Culicoides sonorensis (Diptera: Ceratopogonidae) with emphasis on the significance of animal manure and the associated bacterial community." Diss., Kansas State University, 2016. http://hdl.handle.net/2097/32573.

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Doctor of Philosophy<br>Department of Entomology<br>Ludek Zurek<br>The larval stages of Culicoides sonorensis Wirth and Jones, a confirmed vector of bluetongue and epizootic hemorrhagic disease viruses affecting ruminants in North America, have been observed to occur typically in animal waste enhanced muds. In this dissertation, I studied the larval development (first instar to adult stage) and oviposition (four-choice assays) of C. sonorensis on sterilized mud (autoclaved) enriched with manure of different farm animal species (dairy cattle, beef cattle, sheep, goats, pigs, horses, white-taile
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Cersosimo, Laura M., Melissa L. Bainbridge, Jana Kraft, and André-Denis G. Wright. "Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows." BIOMED CENTRAL LTD, 2016. http://hdl.handle.net/10150/614742.

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Background: Enteric methane from rumen methanogens is responsible for 25.9 % of total methane emissions in the United States. Rumen methanogens also contribute to decreased animal feed efficiency. For methane mitigation strategies to be successful, it is important to establish which factors influence the rumen methanogen community and rumen volatile fatty acids (VFA). In the present study, we used next-generation sequencing to determine if dairy breed and/or days in milk (DIM) (high-fiber periparturient versus high-starch postpartum diets) affect the rumen environment and methanogen community
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39

Almeida, Wallynson Eduardo Silva [UNESP]. "Caracterização parcial do gene 16S rRNA de isolados do solo e seus potenciais na solubilização de fostato e influência no crescimento de soja (Glycine max) e milho (Zea mays)." Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/148901.

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Submitted by WALLYNSON EDUARDO SILVA ALMEIDA null (wallynson@yahoo.com.br) on 2017-02-24T16:09:51Z No. of bitstreams: 1 Dissertação_Wallynson_Eduardo_Silva_Almeida.pdf: 1951842 bytes, checksum: 7628e3c85a4699b4530e564dcd6cc11a (MD5)<br>Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-03-07T12:39:08Z (GMT) No. of bitstreams: 1 almeida_wes_me_jabo.pdf: 1951842 bytes, checksum: 7628e3c85a4699b4530e564dcd6cc11a (MD5)<br>Made available in DSpace on 2017-03-07T12:39:08Z (GMT). No. of bitstreams: 1 almeida_wes_me_jabo.pdf: 1951842 bytes, check
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40

Varna, Klaidas. "Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2007~D_20090908_194033-88862.

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Pienarūgščių bakterijų paieška ir jų identifikavimas migruojančių didžiųjų ančių (Anas platyrhynchos) žarnyne naudojant dalinių 16S rRNR geno sekų analizę ir kultivavimu paremtus metodus Klaidas VARNA Vilniaus Universiteto Ekologijos Institutas, Hidrobiontų Ekologijos ir Fiziologijos Laboratorija bei Populiacinės Genetikos Laboratorija, Akademijos-2, Vilnius-21, 08412, Lietuva. Šiame tyrime pavasarinių ir rudeninių didžiųjų ančių (Anas platyrhynchos) migrantų iš Nemuno deltos virškinamojo trakto pieno rūgšties bakterijų įvairovė buvo ištirta naudojant molekulinius metodus (polimerazės grandini
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Renvoisé, Aurélie. "Applicabilité de la PCR "universelle" 16S comme outil d'identification et de détection bactérienne en laboratoire hospitalier de bactériologie." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM5016/document.

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La PCR universelle ciblant le gène codant pour l'ARNr 16S à l'aide d'amorces universelles, a d'abord été développée pour des études phylogénétiques. En effet ce gène est universellement retrouvé chez les bactéries et sa fonction est conservée. Ainsi, il peut servir d'« horloge moléculaire » pour mesurer les distances phylogénétiques entre les différentes espèces bactériennes. La PCR universelle a ensuite été appliquée en microbiologie clinique dans deux domaines distincts : la détection et l'identification bactériennes. Dans ce travail, nous avons évalué l'applicabilité de la PCR « universelle
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Lira, Évelyn Costa, 991286357, and https://orcid org/0000-0003-1863-7416. "Diversidade microbiana do trato genital feminino." Universidade Federal do Amazonas, 2017. https://tede.ufam.edu.br/handle/tede/6756.

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Submitted by Évelyn Lira (costa_eve@yahoo.com.br) on 2018-11-03T15:34:17Z No. of bitstreams: 3 TESE_Évelyn Costa Lira.pdf: 5199119 bytes, checksum: 3542649866fd36a1d250872b70b7f22e (MD5) Carta de Encaminhamento.pdf: 120410 bytes, checksum: d948e19f5bae8bee2cb485c166efda6d (MD5) Diploma de Doutorado ou Ata de Defesa.pdf: 129558 bytes, checksum: 3cdafbe7ae427bf6a30e4dd767a402e5 (MD5)<br>Approved for entry into archive by PPGBIOTEC Biotecnologia (ppg_biotec.ufam@yahoo.com.br) on 2018-11-06T20:43:46Z (GMT) No. of bitstreams: 3 TESE_Évelyn Costa Lira.pdf: 5199119 bytes, checksum: 3542649866fd3
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Araujo, Gilmara Henriques. "Caracterização bacteriológica da água do mar e diversidade de bactérias cultiváveis associadas ao coral Siderastrea stellata nos recifes costeiros de Cabo Branco, João Pessoa-PB." Universidade Federal da Paraí­ba, 2013. http://tede.biblioteca.ufpb.br:8080/handle/tede/3648.

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Made available in DSpace on 2015-04-01T14:16:02Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 1597259 bytes, checksum: eaba4f2d4108bede1b543c3806717952 (MD5) Previous issue date: 2013-05-22<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>Bacteria play a fundamental role in the health of corals. The interest in the study of microorganisms associated with corals has increased since the confirmation that they can be pathogenic or mutualistic. In the coastal reefs of the State of Paraiba the cases of the pigmentation changes of scleractinian Siderastrea stellata, tha
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Dellias, Marina de Toledo Ferraz. "Comunidade bacteriana dos biofilmes da fermentação alcoólica: estrutura, composição, suscetibilidade aos antimicrobianos e formação de biofilme em culturas puras." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-01042016-091529/.

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A produção de etanol nas destilarias brasileiras é baseada na atividade fermentativa da levedura Saccharomyces cerevisiae que utiliza o caldo da cana-de-açúcar e/ou o melaço como substrato. Bactérias contaminantes da fermentação alcoólica competem com as leveduras pelos açúcares, afetando o rendimento do sistema produtivo e, consequentemente, causando perdas econômicas significativas às usinas. Biofilmes formados nos tanques de fermentação alcoólica agem como reservatórios de bactérias, contribuindo para contaminações persistentes e de difícil controle. Os biofilmes proporcionam aos seus habit
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45

Venturini, Andressa Monteiro. "Efeito do uso do solo e da rizosfera de cana-de-açúcar na estrutura e abundância de comunidades de Bacteria e do filo Verrucomicrobia." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-07082018-102457/.

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A abundância e diversidade dos microrganismos do solo podem ser influenciadas por grande número de fatores, associados, principalmente, ao tipo de solo, sua cobertura e uso. A microbiota do solo apresenta grande importância pelos processos desempenhados pelos seus organismos, essenciais para todos os ecossistemas terrestres. Apesar da grande complexidade associada a esses estudos, os fatores que influenciam as comunidades microbianas podem ser melhor elucidados pela pesquisa com grupos específicos. Nesse sentido, o filo bacteriano Verrucomicrobia, grupo ubíquo em solos, apresenta elevada abund
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46

Garcia, Carlos Eduardo. "Isolamento e identificação de actinobacterias em solos de terra preta antropogenica (TPA) da Amazonia Central por ARDRA e sequenciamento do gene 16S rRNA." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256616.

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Orientadores: Fumio Yokota, Tasi Siu Mui<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos<br>Made available in DSpace on 2018-08-05T16:40:59Z (GMT). No. of bitstreams: 1 Garcia_CarlosEduardo_D.pdf: 3424911 bytes, checksum: 6338d2ede1846761521ec0cfb4c5e1ec (MD5) Previous issue date: 2006<br>Doutorado<br>Doutor em Ciência de Alimentos
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Nobrega, Letícia Maria Menezes 1983. "Estudo da diversidade bacteriana de canais radiculares infectados em casos de abscesso apical agudo por cultura, clonagem e sequenciamento do gene 16S rRNA." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290434.

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Orientador: Brenda Paula Figueiredo de Almeida Gomes<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba<br>Made available in DSpace on 2018-08-22T06:43:53Z (GMT). No. of bitstreams: 1 Nobrega_LeticiaMariaMenezes_D.pdf: 2252800 bytes, checksum: 31910f0e934110c7e863efa8d0e293da (MD5) Previous issue date: 2012<br>Resumo: Abscessos apicais agudos são condições frequentes na urgência endodôntica. São infecções polimicrobianas que apresentam variações entre indivíduos, e afetam o sistema de canais radiculares e tecidos periapicais. Associações bacterian
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Bauer, Franziska Romana [Verfasser], Jürgen [Akademischer Betreuer] Geist, Jürgen [Gutachter] Geist, and Tanja [Gutachter] Gschlößl. "Characterization of cyanobacterial communities of Bavarian lakes based on the 16S rRNA Gene / Franziska Romana Bauer ; Gutachter: Jürgen Geist, Tanja Gschlößl ; Betreuer: Jürgen Geist." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1172415072/34.

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49

Sjöholm, Billie, and Babak Bahrami. "Prevalence and Identification of Lactobacillus Species Isolated from Infected Root Canals by MALDI-TOF Mass Spectrometry, 16S rRNA Gene Sequencing and API 50 CHL." Thesis, Umeå universitet, Tandläkarutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-97871.

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Lactobacillus, a part of the commensal oral microflora, is frequently found in infected root canals but is not considered to be an endodontic pathogen. Lactobacilli have proven to be difficult to identify on species level with biochemical and gene sequencing methods. MALDI-TOF is a new identification method and to our knowledge it has not been used on lactobacilli from infected root canals. The aims of this study were to evaluate the prevalence of lactobacilli in infected root canals and to examine if MALDI-TOF is a suitable method for identifying lactobacilli species. In the retrospective stu
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Hussein, Khwam Reissan. "Source tracking of faecal indicator bacteria of human pathogens in bathing waters : an evaluation and development." Thesis, University of Plymouth, 2014. http://hdl.handle.net/10026.1/3011.

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Bacterial water pollution is a significant problem because it is associated with reduction in the ‘quality’ of water systems with a potential impact on human health. Faecal indicator bacteria (FIB) are usually used to monitor the quality of water, and to indicate the presence of pathogens in water bodies. However, enumeration alone does not enable identification of the precise origin of these pathogens. This study aimed to monitor the quality of bathing water and associated fresh water in and out of the ‘bathing season’ in the UK, and to evaluate the use of microbial source tracking (MST) such
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