Dissertations / Theses: 'Plastocyanine'

1

Durell, Stewart Richard. "Biophysical studies of plastocyanin /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487673114114086.

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2

He, Shiping. "Protein engineering of pea plastocyanin." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295349.

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3

Hani, Umama. "Regulation of cyclic and pseudocyclic electron transport." Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPASB044.

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La photosynthèse, principale voie de production d'énergie dans les environnements naturels, repose sur des flux d'électrons intervenant dans plusieurs complexes dans la membrane des thylakoïdes des organismes photosynthétiques. Le flux principal est le transport « linéaire » des électrons qui implique leur transfert de l'eau au NADP⁺, le tout couplé à la synthèse d'ATP. L'oxydation de l'eau photosynthétique est catalysée par les clusters de manganèse (Mn₄CaO₅) au niveau du photosystème II (PSII). Pour assurer un équilibre optimal entre la quantité d'énergie produite et consommée, les organismes photosynthétiques détournent une partie de l'énergie lumineuse récoltée des voies de transport d'électrons "linéaires" vers des voies "alternatives". Parmi ces voies, on trouve les transports cyclique et pseudocyclique des électrons autour du photosystème I (PSI), qui fournit de l'ATP supplémentaire pour répondre aux besoins métaboliques. En outre, des systèmes redox spécialisés appelés "thiorédoxines" sont responsables du maintien de l'état redox et de l'acclimatation rapide des plantes à un environnement changeant. Dans le cas contraire, cela peut conduire à des niveaux toxiques d'espèces réactives de l'oxygène (ROS) dans les cellules. Nous avons étudié les effets de l'excès et de la carence en manganèse (Mn) sur le transport des électrons au cours de la photosynthèse chez l'hépatique Marchantia polymorpha. Nous avons montré que l'homéostasie du Mn a un effet sur le métabolisme mais aussi sur la photosynthèse. De plus, nous avons étudié les changements redox in vivo du P700 et du la plastocyanine (PC) en utilisant le spectrophotomètre KLAS-NIR. Il semble que la carence en Mn permet une augmentation du transport cyclique des électrons (TCE) ce qui indique la présence de supercomplexes contenant le PSI et le complexe du cytochrome b6f. Dans un second temps, nous nous sommes concentrées sur la régulation redox de la réduction de l'oxygène (transport d'électrons pseudocyclique) du côté de l'accepteur du PSI. En utilisant la spectroscopie RPE par piégeage indirect de spin, nous avons montré que des plantes sauvages d'Arabidopsis thaliana génèrent plus de ROS en photopériode de jour court (JC) qu'en photopériode de jour long (JL). En outre, nous avons mis en évidence le rôle de plusieurs acteurs, y compris les thiorédoxines et plusieurs protéines du lumen et du stroma dans la régulation redox. De plus, j'ai découvert que le transfert du pouvoir réducteur du stroma au lumen est médié par une protéine appelée CCDA. Par ailleurs, l'attachement réversible de Trxm à la membrane des thylakoïdes agit comme une force motrice pour l’accumulation des ROS en JC. Dans l'ensemble, les résultats établissent un lien étroit entre le transport cyclique et pseudocyclique des électrons en termes de régulations redox médiées par les thiorédoxines. Une voie est également ouverte quant à une exploration plus approfondie du TCE dans différentes conditions de stress
Photosynthesis acts as the main gateway for energy production in natural environments and relies on the electron flow via several complexes in the thylakoid membrane of photosynthetic organisms. The major flux is “linear” electron transport, which involves the transfer of electrons from water to NADP⁺, coupled with the ATP synthesis. Photosynthetic water oxidation is catalyzed by manganese cluster (Mn₄CaO₅) at photosystem II (PSII). To ensure an optimal balance between the amount of energy produced and consumed, photosynthetic organisms divert part of the harvested light energy from “linear” to “alternative” electron transport pathways. Among those pathways are cyclic and pseudocyclic electron transport around Photosystem I (PSI), which supplies extra ATP to meet metabolic demands. Moreover, specialized redox systems, called " thioredoxins " are responsible for maintaining the redox status and fast acclimation of plants to constantly fluctuating environments, which could otherwise lead to toxic levels of reactive oxygen species (ROS) production. We studied the effects of manganese (Mn) excess and deficiency on photosynthetic electron transport in the liverwort Marchantia polymorpha. We have shown that Mn homeostasis has an effect at both metabolic and photosynthetic levels. Moreover, we have studied the in vivo redox changes of P700 and PC using KLAS-NIR spectrophotometer and have shown that Mn deficiency seems to enhance cyclic electron transport (CET), that may indicate the presence of supercomplexes containing PSI and cytochrome b6f complex. The second part of this PhD focused on the redox regulation of oxygen reduction (pseudocyclic electron transport) at the PSI acceptor side. By using indirect spin trapping EPR spectroscopy, we have shown that Arabidopsis thaliana wild type plants generate more ROS in short day (SD) photoperiod than in long day (LD) photoperiod. Further, the current study highlighted the role of several players in redox regulation; including thioredoxins and several other lumenal and stromal proteins. Moreover, I explored that the transfer of reducing powers from stroma to lumen is mediated by a protein called CCDA and that reversible attachment of Trxm to the thylakoid membrane acts as the driving force for higher ROS under the SD light regime. Overall, this research establishes a strong connection between cyclic and pseudocyclic electron transport in terms of thioredoxins mediated redox regulations and also paves the way to further explore CET under different stress conditions

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4

Driscoll, P. C. "'1H NMR studies of plastocyanin in solution." Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382519.

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5

Chua, Y. L. "Chromatin structure of the pea plastocyanin gene." Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597674.

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The pea plastocyanin gene (PetE) is a single-copy, nuclear photosynthesis gene. Pea PetE is flanked by an enhancer/5' matrix attachment region (MAR) and a 3' MAR. When linked upstream to uidA directed by the CaMV 35S promoter, the enhancer/5' MAR increased reporter gene expression in transgenic tobacco plants. In contrast, the 3' MAR increased expression only when linked downstream of the reporter gene. The 3' MAR, but not the 5' MAR, decreased variation in reporter gene expression. These results indicate that the two MARs surrounding PetE have different effects on transgene expression. The chromatin structure of PetE was examined at three different transcriptional states by investigating the nuclease accessibility of the gene in pea roots, etiolated shoots and green shoots. Time-course digestions of nuclei with micrococcal nuclease and DNaseI indicated that the enhancer/5' MAR and promoter regions were more resistant to digestion in the inactive gene in pea roots than the same regions in the active gene in shoots, whereas the transcribed region of PetE was digested similarly amongst the tissues. PetE transcription is hence accompanied by changes in the nuclease accessibility of the enhancer/5' MAR and promoter regions only. The acetylation states of histone H3 and H4 proteins associated with PetE were analysed by chromatin immunoprecipitation with antibodies specific for acetylated or non-acetylated histone tails followed by polymerase chain reaction quantification. Comparison of pea tissue indicated that histone acetylation was associated with increased PetE transcription in green shoots. Moreover, acetylation of both histone H3 and H4 proteins was targeted to the enhancer/5' MAR and promoter regions in green shoots, suggesting that only specific nucleosomes along the gene were modified.

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Wagner, Michael Johannes. "Interaction between cyanobacterial plastocyanin and cytochrome f." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627130.

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7

Javadi, Morteza. "Plastocyanin evaluation in wheat (T. aestivum L.)." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu1298400458.

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8

Bottin, Hervé. "Etude du transfert d'electron dans le photosysteme 1 des vegetaux superieurs par spectroscopie d'absorption par eclairs." Paris 6, 1987. http://www.theses.fr/1987PA066147.

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9

Helliwell, Christopher Andrew. "Regulation of expression of the pea plastocyanin gene." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338311.

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10

Last, David Ian. "Structure and expression of the pea plastocyanin gene." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.256631.

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11

Brown, Naomi Jane. "Post-transcriptional regulation of the pea plastocyanin gene (PetE)." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597002.

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Expression of the pea plastocyanin gene (PetE) is regulated both by light and by signals from the chloroplast. Previous work has indicated that the light and chloroplast-controlled regulation operates post-transcriptionally in transgenic tobacco, requiring the correct 5’ terminus of the transcript and elements within the plastocyanin-coding region. The post-transcriptional light and chloroplast-controlled regulation of pea PetE has now been demonstrated to operate in transgenic Arabidopsis plants, indicating that the regulation is conserved in an additional plant species. The overall aim of the research described in this dissertation was to investigate the mechanisms by which light and plastid signals influence the stability of PetE transcripts. PetE constructs containing premature stop codons in the coding region were generated to investigate whether translation has a role in the light or chloroplast-controlled regulation. RNA-gel-blot analysis of transgenic plants containing these constructs was used to examine the effects of light and plastid inhibitors on pea PetE transcript accumulation in 7-day-old tobacco seedlings. The results obtained suggested that translation of the start of the PetE coding region is required for both light and plastid-regulated transcript stability. Constructs containing progressive 3’ deletions of the PetE coding region, fused to the Luc reporter gene, were generated to examine how much of the coding sequence is necessary for the regulation. Luciferase assays and RNA-gel-blot analysis were carried out on transgenic tobacco seedlings containing the constructs, to examine the effects of light and plastid inhibitors on the regulation. The results indicated that an element important in the light and chloroplast-controlled regulation is located in the first 12% of the coding region, corresponding to the first 60 nucleotides. The start of the plastocyanin-coding region therefore appears to contain sequences important in the regulation by light and plastid signals, and these sequences may need to be translated in order for the regulation to operate.

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12

Hunter, David M. "Studies of electron transfer in plastocyanins by nuclear magnetic resonance." Thesis, University of Newcastle Upon Tyne, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270171.

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13

Fisher, N. E. "The interaction between cytochrome f and plastocyanin from higher plants." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599049.

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The aims of this study were to investigate the nature of the interaction between cytochrome f and plastocyanin isolated from higher plants. The reaction kinetics for these redox partners isolated from two homologous systems (spinach and turnip) were investigated. Limited proteolysis of purified spinach cytochrome f with proteinase K generated a form of the protein that was essentially kinetically identical to soluble, monomeric turnip cytochrome f in the kinetics of its reaction with plastocyanin. The second order rate constant for the reaction between oligomeric, nonproteolysed spinach cytochrome f and spinach plastocyanin (7.19 x 10⁷ M^-1 s^-1) was 1.9-fold less than that observed for the reaction between proteinase K-treated spinach cytochrome f and spinach plastocyanin (13.7 x 10⁷ M^-1 s^-1). The reactivity of cytochrome f when present in isolated cytochrome bf complex towards plastocyanin (k₂ = 7.78 x 10⁷ M^-1 s^-1 for the spinach plastocyanin/spinach bf reaction and 8.68 x 10⁷ M^-1 s^-1 for the turnip plastocyanin/turnip bf reaction) was very similar to that of unproteolysed spinach cytochrome f. The difference in rate between proteolysed and non-proteolysed cytochrome f in its reaction with plastocyanin is likely to be due to the occlusion of a region of the cytochrome involved in interacting with plastocyanin, caused by aggregation of the non-proteolysed form of the protein. This may also explain the decrease in rate observed for the reaction between bf complex and plastocyanin compared to the reaction with soluble cytochrome f. The reaction kinetics for homologous (turnip cytochrome f/ turnip plastocyanin) and heterologous (turnip cytochrome f/spinach plastocyanin) systems were very similar (k₂ = 14.31 x 10⁷ and 12.9x 10⁷ M^-1 s^-1 respectively at pH 6.0. and 100 mM ionic strength). It is concluded that heterologous higher plant systems are valid for examining the reaction between cytochrome f and plastocyanin on the condition that the cytochrome is isolated from a cruciferous source. Mutation of Y83 (a residue suggested to be essential for the electron transfer activity of the protein) to serine, glutamate and tryptophan generated structurally unstable mutants which lost copper during the purification process. Similar instability was observed when the surface exposed copper ligand H87 was mutated to cysteine. Introduction of leucine at position 90 (adjacent to H87), replacing alanine, resulted in a stable mutant.

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14

Sandberg, Anders. "Folding of cupredoxins : a case study of azurin and plastocyanin /." Göteborg : Göteborg university, 2004. http://catalogue.bnf.fr/ark:/12148/cb40111222z.

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15

Lawler, Anne. "Investigations of blue copper proteins and their active site variants." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366595.

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16

Sandhu, Jagdeep Singh. "An A/T-rich enhancer in the pea plastocyanin gene promoter." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627061.

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17

Haddadian, Esmael Jafari. "Brownian dynamics study of cytochrome f / Rieske interactions with cytochrome c₆ and plastocyanin." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1123695434.

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Thesis (Ph. D.)--Ohio State University, 2005.
Title from first page of PDF file. Document formatted into pages; contains xxiii, 184 p.; also includes graphics (some col.). Includes bibliographical references (p. 169-184). Available online via OhioLINK's ETD Center

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18

Jafari, haddadian Esmael. "Brownian dynamics study of cytochrome f / Rieske interactions with cytochrome c6 and plastocyanin." The Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1123695434.

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19

Woo, Edward Samuel. "Nucleotide sequence and phylogeny of a plastocyanin gene in the marine diatom, «Thalassiosira oceanica»." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32418.

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Diatoms are thought to have acquired an Fe-containing cytochrome (cyt) c6 to transfer electrons between cyt b6f and photosystem (PS) I of the photosynthetic apparatus like other chlorophyll a/c-containing phytoplankton. Here we report the isolation and cloning of a plastocyanin gene from Thalassiosira oceanica (CCMP 1005). The gene encoded a Cu-containing protein that is known in other organisms to functionally replace the Fe-containing cyt c6. The inferred protein sequence had the highest identity with the green haptophyte, Emiliania huxleyi, and possessed many of the globular properties necessary for function and interaction with upstream and downstream partners. Eleven strains of oceanic and coastal diatoms were screened for the presence of the plastocyanin gene using degenerate primers: one other species was observed to contain the gene; T. oceanica (CCMP 1006). Phylogenetic analysis of the 5.8 rRNA gene of these species showed that both T. oceanica strains with plastocyanin were closely related to each other. The cloned sequence of T. oceanica (CCMP 1006) contained greater than 80% of the protein-coding region and shared 99% nucleotide identity and 100% conserved unique intronic region. The inferred protein sequence of this species had 100% identity with the inferred protein sequence of T. oceanica (CCMP 1005).
Durant l'évolution de la photosynthèse, il est pensé que les diatomées ont acquis cytochrome (cyt) c6, une protéine contenant un atome de Fe, pour le transfert des électrons entre le complexe cyt b6f et le photosystème (PS) I de l'appareil photosynthetique, comme d'autres phytoplanctons ayant les chlorophyll a/c. Ici, nous rapportons l'isolement et clonage du gène de plastocyanin du diatomée Thalassiosira oceanica (CCMP 1005). Le gene codait une protéine contenant un atome de Cu qui est connue de remplacer fonctionellement cyt c6 (contenant un atome de Fe). La sequence inférée de la proteine montrait la plus grande identité avec Emiliania huxleyi, un haptophyte vert (green haptophyte), et possèdait de nombreuses propriétés globulaires nécessaires aux fonctions et intérections avec les protéines partenaires en amont et en aval. Onze espèces de diatomées océanique et côtières ont été examinées pour la présence du gène codant pour plastocyanin en utilisant les amorces dégénérées (degenerate primers): une autre espèce a révélé la présence du gène de plastocyanin; T. ocenica (CCMP 1006). L'analyse phylogénétique des gènes 5.8 rRNA de cet espèce a démontré que la souche de T. oceanica possèdant le plastocyanin sont étroitement reliées entre elles. Les séquences clônées de T. oceanica (CCMP 1006) recelaient plus de 80% de la région cryptant la protéine et partageaient 99% des nucléotides et 100% des régions introniques uniques. La séquence de protéine inférée de ces espèces de phytoplanctons ont démontré 100% d'identité avec celle inférée pour T. oceanica (CCMP 1005).

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20

Varley, Janet Penelope Anne. "Studies of plastocyanin and a putative iron-regulated protein from the cyanobacterium Phormidium laminosum." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318293.

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21

Baranova, Maria V. "Protein-Protein Interactions and Electron Transfer Associated with Cytochrome F and Plastocyanin From the Cyanobacterium Prochlorothrix Hollandica." Bowling Green State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1178243290.

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22

Javadi, Morteza. "Plastocyanin evaluation in wheat (T. aestivum L.) as affected by rates of phosphate and copper /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487590702993016.

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23

Schlarb, Beatrix Gabriele. "The interaction between cytochrome f and plastocyanin : a comparison between higher plants and the cyanobacterium Phormidium laminosum." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621846.

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24

Anderson, George Philip. "An investigation of plastocyanin's binding sites for cytochrome f and Photosystem I by chemical modification /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487268021747963.

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25

Granlund, Irene. "Proteomic analysis of Arabidopsis thaliana." Doctoral thesis, Umeå : Department of Chemistry, Umeå University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1820.

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26

Sanderson, Douglas Grant. "An investigation of the relationship between the structure and function of the blue copper electron transport protein plastocyanin using thin-layer, steady-state spectroelectro-chemistry /." The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487262513407884.

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27

Vorst, Oscar Frederik Jozef. "Regulated expression of the nuclear photosynthesis genes ferredoxin and plastocyanin = De gereguleerde expressie van de nucleaire fotosynthese genen ferredoxine en plastocyanine /." 1992. http://www.gbv.de/dms/bs/toc/131124951.pdf.

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28

Weigel, Martin Christian [Verfasser]. "Molekulargenetische und physiologische Analysen von Plastocyanin und Cytochrom-cx-Mutanten bei Arabidopsis thaliana / vorgelegt von Martin Christian Weigel." 2006. http://d-nb.info/979203767/34.

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29

Sommer, Frederik [Verfasser]. "Reverse genetics of PsaA and PsaB to dissect their function in binding and electron transfer from plastocyanin or cytochrome c6 to the core of photosystem 1 / von Frederik Sommer." 2003. http://d-nb.info/970666071/34.

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Dissertations / Theses on the topic 'Plastocyanine'

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