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1

Bartram, Jamie, Joseph Cotruvo, Al Dufour, Stan Hazan, and Bob Tanner. "Heterotrophic Plate Count." International Journal of Food Microbiology 92, no. 3 (2004): 239–40. http://dx.doi.org/10.1016/j.ijfoodmicro.2003.08.004.

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2

Harwani, Dharmesh. "The Great Plate Count Anomaly and the Unculturable Bacteria." International Journal of Scientific Research 2, no. 9 (2012): 350–51. http://dx.doi.org/10.15373/22778179/sep2013/122.

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3

Day, N. B., B. J. Skura, and W. D. Powrie. "Comparison of three media used for the enumeration of heat-injured Botrytis cinerea." Canadian Journal of Microbiology 34, no. 2 (1988): 194–96. http://dx.doi.org/10.1139/m88-036.

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The efficacy of three media (antibiotic-supplemented plate count agar (3 days, 21 °C); antibiotic–pyruvate–supplemented plate count agar (3 days, 21 °C); and trypticase soy agar (16 h, followed by 2.5 days on antibiotic-supplemented plate count agar, 21 °C)) for recovery of heat-injured Botrytis cinerea spores was compared using hydrophobic grid membrane filters. The filters restrict spreading of fungal colonies and facilitate the transfer of fungal spores from pre-enrichment trypticase soy agar to antibiotic-supplemented plate count agar. Counts obtained from pre-enrichment on trypticase soy
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4

SHAW, B. G., C. D. HARDING, W. H. HUDSON, and L. FARR. "Rapid Estimation of Microbial Numbers on Meat and Poultry by the Direct Epifluorescent Filter Technique." Journal of Food Protection 50, no. 8 (1987): 652–57. http://dx.doi.org/10.4315/0362-028x-50.8.652.

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The direct epifluorescent filter technique (DEFT) for rapid estimation of microbial numbers was evaluated by comparison with the plate count on a variety of uncooked red meat and poultry samples. Good agreement [correlation coefficient (r) = 0.95–0.96] was obtained from samples with plate counts of 5 × 103/g or /cm2 and above from red meat carcasses (surface swabbed), aerobic or vacuum packed chill-stored joints (surface sampled - stomachered) and frozen beef (thawed stomachered). For stored and unstored raw poultry sampled by skin scraping or stomachering of muscle and skin good overall corre
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5

Roth, Jonathan N. "Temperature-Independent Pectin Gel Method for Aerobic Plate Count in Dairy and Nondairy Food Products: Collaborative Study." Journal of AOAC INTERNATIONAL 71, no. 2 (1988): 343–49. http://dx.doi.org/10.1093/jaoac/71.2.343.

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Abstract Ten laboratories participated in a collaborative study to compare the pectin-based plate count (PC) Redigel method with the aerobic plate count and standard plate count agar-based standard methods for the estimation of total bacterial counts in 9 different nondairy food and dairy food products. The foods were cream, homogenized milk, raw milk, cheese, raw chicken, raw oysters, frozen broccoli, flour, and spices. Each laboratory analyzed 6 samples (3 sample pairs) of each food group. Counts obtained by the pectin-based plate count and agarbased plate count methods differed significantl
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6

SMITH, LORRAINE B., TERRANCE L. FOX, and F. F. BUSTA. "Comparison of a Dry Medium Culture Plate (Petrifilm SM Plates) Method to the Aerobic Plate Count Method for Enumeration of Mesophilic Aerobic Colony-Forming Units in Fresh Ground Beef." Journal of Food Protection 48, no. 12 (1985): 1044–45. http://dx.doi.org/10.4315/0362-028x-48.12.1044.

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Mesophilic aerobic microbial populations in fresh ground beef were enumerated with a new system, Petrifilm™ SM Plates (PSM), and with the conventional aerobic plate count (APC) method using standard methods agar (SMA). Total colony-forming units were determined in 119 fresh ground beef samples (29 extra-lean, 30 lean and 60 regular) purchased at nine different retail markets over a period of 6 wk. Linear regression analysis of PSM vs. APC counts gave a slope of 0.963, an intercept of −0.027, and a correlation coefficient of 0.951. Mean log10 counts on PSM were 5.86 compared to 6.11 on SMA (P&a
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7

ELLENDER, RUDOLPH D., SANDRA L. SHARP, PAUL G. COMAR, and ROBERT P. TETTLETON. "Rapid Methods to Evaluate the Bacteriological Quality of Frozen Crabmeat." Journal of Food Protection 56, no. 6 (1993): 545–47. http://dx.doi.org/10.4315/0362-028x-56.6.545.

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The standard methods plate count (SMPC) of frozen crabmeat samples was compared with counts of two alternative aerobic plate count methods (Redigel, Petrifilm). The differences in counts were compared after incubation at two temperatures (35°C and room temperature; RT) and three intervals of time (24, 48, and 72 h). No statistical differences were found when the time of analysis or the method of analysis was compared. However, differences were observed within SMPC values and within Petrifilm plate count values when RT was compared to 35°C, Redigel plate counts at RT and 35°C were not significa
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8

ADKINSON, ROBERT W., RONALD H. GOUGH, and JEFFREY J. RYAN. "Use of Individual, Premoistened, Disposable Wipes in Preparing Cow Teats for Milking and Resultant Raw Milk Quality and Production1." Journal of Food Protection 54, no. 12 (1991): 957–59. http://dx.doi.org/10.4315/0362-028x-54.12.957.

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Two methods of preparing cows for milking were compared. One preparation consisted of wiping each teat clean with individual, premoistened, disposable wipes. This method was compared with washing teats with a hand-held water nozzle and drying with individual paper towels. Two groups of eight Holstein cows each were randomly assigned to the two treatments. Aseptically collected weigh jar milk samples from individual cow milkings were analyzed for standard plate count, preliminary incubation count, laboratory pasteurization count, and coliform count. Pretrial bacterial counts were monitored for
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9

CHAIN, VICKI S., and DANIEL Y. C. FUNG. "Comparison of Redigel, Petrifilm, Spiral Plate System, Isogrid, and Aerobic Plate Count for Determining the Numbers of Aerobic Bacteria in Selected Foods." Journal of Food Protection 54, no. 3 (1991): 208–11. http://dx.doi.org/10.4315/0362-028x-54.3.208.

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The numbers of aerobic bacteria from chicken, ground beef, ground pork, shelled pecan, raw milk, thyme, and flour (20 samples from each food) were determined by four alternative viable cell count methods (Redigel, Petrifilm, Spiral Plate System, and Isogrid) to ascertain the effectiveness of these methods in providing viable cell counts compared with the widely used Aerobic Plate Count (APC) method. The results indicated that all five methods were highly comparable (r=0.97 and higher, with the exception of Petrifilm versus Spiral Plate System, which was 0.88) and exhibited a high degree of acc
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10

BONVEHI, JOSEP SERRA, and ROSSEND ESCOLÁ JORDÁ. "The Microbiological Quality of Honey as Determined by Aerobic Colony Counts." Journal of Food Protection 56, no. 4 (1993): 336–37. http://dx.doi.org/10.4315/0362-028x-56.4.336.

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The number of mesophilic aerobic colonies was determined in 72 samples of mono- and multifloral honey from various sources by the plate count and the membrane filter methods. The presence of motile colonies made the plate counts unreliable. The microorganism producing these colonies was identified as Bacillus alvei. Colony counts could only be carried out in 27 of the samples when using the plate count method, while with the membrane filter method the number of colonies was counted in all the samples.
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11

Townsend, David E., and Ali Naqui. "Comparison of SimPlate TotalTM Plate Count Test with Plate Count Agar Method for Detection and Quantitation of Bacteria in Food." Journal of AOAC INTERNATIONAL 81, no. 3 (1998): 563–70. http://dx.doi.org/10.1093/jaoac/81.3.563.

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abstract The SimPlateTM Total Plate Count (TPC) test, developed by IDEXX Laboratories, Inc., detects and quantitates total bacterial concentration in food after 24 h of incubation. The performance of SimPlate TPC was compared with that of the plate count agar (PCA) method for enumerating total bacterial concentration of 255 food samples representing 15 different food matrixes. Total bacterial counts on SimPlate TPC were measured after 24 h of incubation and plotted against values obtained from PCA after 48 h. Simple regression analysis of the data showed strong correlation between the methods
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12

COOK, DAVID W. "Refrigeration of Oyster Shellstock: Conditions Which Minimize the Outgrowth of Vibrio vulnificus." Journal of Food Protection 60, no. 4 (1997): 349–52. http://dx.doi.org/10.4315/0362-028x-60.4.349.

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The multiplication of Vibrio vulnificus in summer harvest oyster shellstock held without refrigeration was followed over a 14 h postharvest period. Mean (n = 7) increases were 0,75, 1.30, 1.74, and 1.94 log units at 3.5 h, 7 h, 10.5 h, and 14 h postharvest, respectively. Aerobic plate counts (spread plates on plate count agar [PCA] containing 1% NaCl, 25°C) but not standard plate counts (pour plates, PCA, 35°C) showed a similar trend in increase. Reducing the time oyster shellstock remains outside refrigeration can decrease consumer exposure to high numbers of V. vulnificus, but shellstock mus
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13

Jackson, R. Wayne, Karen Osborne, Gary Barnes, et al. "Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water." Applied and Environmental Microbiology 66, no. 1 (2000): 453–54. http://dx.doi.org/10.1128/aem.66.1.453-454.2000.

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ABSTRACT A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95;y = 0.99X + 0.06).
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14

Entis, Phyllis, J. Allen, A. Bhatnagar, et al. "Hydrophobic Grid Membrane Filter Method for Aerobic Plate Count in Foods: Collaborative Study." Journal of AOAC INTERNATIONAL 69, no. 4 (1986): 671–76. http://dx.doi.org/10.1093/jaoac/69.4.671.

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Abstract Twenty-one laboratories participated in a collaborative study to validate a hydrophobic grid membrane filter (HGMF) method for aerobic plate count by comparing its performance against the AOAC/APHA pour plate method. Raw milk, raw poultry, whole egg powder, flours, and spices were included in the study. Counts obtained by the HGMF and pour plate methods did not differ significantly, except in the case of whole egg powder, for which the HGMF method produced significantly higher counts. The hydrophobic grid membrane filter method for aerobic plate count in foods has been adopted officia
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15

TING, WEI-TSYI, and GEORGE J. BANWART. "Enumeration of Enterococci and Aerobic Mesophilic Plate Count in Dried Soup Using Three Reconstitution Methods." Journal of Food Protection 48, no. 9 (1985): 770–71. http://dx.doi.org/10.4315/0362-028x-48.9.770.

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A naturally contaminated dried soup sample was reconstituted by three different methods (1:1 swirl, 1:9 soak and 1:9 rapid rehydration) and analyzed for enterococci on m-enterococcus agar and aerobic mesophilic plate count on plate count agar. The enterococcal counts obtained by the 1:1 swirl and the 1:9 soak methods were 41.6% and 26.5%, respectively, higher than that of the commonly used 1:9 rapid rehydration method. The aerobic mesophilic plate counts for the three systems were not significantly different.
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16

Vulindlu, M., A. Charlett, S. Surman, and J. V. Lee. "Comparison of agar-based methods for the isolation and enumeration of heterotrophic bacteria with the new Multidose IDEXX(tm) SimPlate method." Water Science and Technology 50, no. 1 (2004): 277–80. http://dx.doi.org/10.2166/wst.2004.0067.

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Pour and spread plates are the conventional methods of choice for the isolation and enumeration of heterotrophic microorganisms in treated water supplies. The tests are performed at 22°C and 37°C for 72 h and 48 h respectively. Counts at 22°C are associated with pollution of water systems from external sources, while counts at 37°C are used as an indication of treatment plant performance and the deterioration of the general quality of water. Conventional methods using Yeast Extract Agar for a pour plate and R2A agar for a spread plate were compared with the multidose IDEXXTM SimPlate method fo
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17

BAILEY, J. S., and N. A. COX. "Evaluation of the Petrifilm SM and VRB Dry Media Culture Plates for Determining Microbial Quality of Poultry." Journal of Food Protection 50, no. 8 (1987): 643–44. http://dx.doi.org/10.4315/0362-028x-50.8.643.

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A self-contained sample-ready system, Petrifilm, which has been developed as an alternative method to the standard aerobic plate count (SPC) and coliform counts as determined by violet red bile (VRB) pour plates, was evaluated for the first time with poultry samples. Swab samples were taken of 109 broiler carcasses at various degrees of freshness, and SPC and VRB pour plates were compared to Petrifilm counts. The correlation coefficient of log10 SPC and log10 Petrifilm SM count was 0.926 with a regression line slope of 1.009 and an intercept of −0.106. A correlation was not determined with col
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18

Planidin, N. P., and T. E. Reimchen. "Spatial, sexual, and rapid temporal differentiation in neuromast expression on lateral plates of Haida Gwaii threespine stickleback (Gasterosteus aculeatus)." Canadian Journal of Zoology 97, no. 11 (2019): 988–96. http://dx.doi.org/10.1139/cjz-2019-0005.

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Lateral lines, a major sensory modality in fishes, are diverse among taxa, but their intraspecific variation has received limited attention. We examined numbers of superficial neuromasts on the buttressing lateral plates (LP) of 1910 threespine stickleback (Gasterosteus aculeatus Linnaeus, 1758) from 26 ecologically and morphologically diverse populations on the Haida Gwaii archipelago, western Canada. Extending from previous studies, we predicted that (i) highly stained dystrophic localities would have threespine stickleback with elevated numbers of neuromasts per plate due to a greater relia
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19

Souto, Luís I. M., Clarice Y. Minagawa, Evelise O. Telles, et al. "Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media." Journal of Dairy Research 77, no. 1 (2009): 63–70. http://dx.doi.org/10.1017/s0022029909990409.

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Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards
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20

Medline Maduka, Chinonye, Akuma Oji, Ugochi Queen Fineboy, and Gideon Chijioke Okpokwasili. "Microbial implications of beef fat and pork fat in the environment." Bionatura 5, no. 4 (2020): 1371–74. http://dx.doi.org/10.21931/rb/2020.05.04.15.

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Developing countries are known to dispose of waste indiscriminately into their environment, off which fat is one of them. These fats release awful odor making passersby uncomfortable and also breeds microorganisms. Environmental factors such as rainfall, sunlight, and wind aid the migration of these fats to other sites, thereby leading to contamination. Total heterotrophic plate count of pork fat ranged from 4.0 x 105 cfu/g to 4.2 x 105 cfu/g and its total coliform plate count was from 3.8 x 105 cfu/g to 4.0 x105 cfu/g while the total heterotrophic plate count of beef fat ranged from 3.1 x 105
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KANEKO, Seiichi, Yoshihiro SATO, Hikaru MINEO, Ken-ichi KOHZAKI, and Fumiaki AKAHORI. "Simple-methods of estimation of standard plate count, count of enterobacteriaceae (coliform count) and count of yeasts and molds." JAPAN JOURNAL OF VETERINARY INFORMATICS 1987, no. 19 (1987): 9–14. http://dx.doi.org/10.2743/jve1986.1987.19_9.

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Bird, Patrick, Jonathan Flannery, Erin Crowley, James Agin, David Goins, and Robert Jechorek. "Evaluation of the 3M™ Petrifilm™ Rapid Yeast and Mold Count Plate for the Enumeration of Yeast and Mold in Food: Collaborative Study, First Action 2014.05." Journal of AOAC INTERNATIONAL 98, no. 3 (2015): 767–83. http://dx.doi.org/10.5740/jaoacint.15-006.

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Abstract The 3M™ Petrifilm™ Rapid Yeast and Mold (RYM) Count Plate is a simple, ready-to-use chromogenic culture method for the rapid detection and enumeration of yeast and mold in food products. The 3M Petrifilm RYM Count Plate method was compared to the U. S. Food and Drug Administration Bacteriological Analytical Manual (FDA BAM) Chapter 18, Yeasts, Molds and Mycotoxins and the ISO 21527:2008 Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Enumeration for Yeast and Molds – Part 1: Colony Count Technique in Products with Water Activity Greater Than 0.95 and Part 2: C
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NIEUWENHOF, F. F. J., and J. D. HOOLWERF. "Impedance Measurement as an Alternative to the Plate Count Method for Estimating the Total Count of Bacteria in Raw Milk." Journal of Food Protection 50, no. 8 (1987): 665–68. http://dx.doi.org/10.4315/0362-028x-50.8.665.

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An improved impedance method is described with a good standard deviation of repeatability (sm = 0.05 log unit) and a fair standard deviation of the estimate of the plate count from the detection time [(sy)x = 0.33 log unit]. Compared with the standard deviation of repeatability of the plate count method (0.07 log unit), the standard deviation of repeatability of the impedance method described is a significant improvement. The impedimetric experiments were done with a Bactometer M123. The detection times as measured by this instrument were compared with the plate counts at 30°C for samples of r
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Rygala, Anna, Joanna Berlowska, and Dorota Kregiel. "Heterotrophic Plate Count for Bottled Water Safety Management." Processes 8, no. 6 (2020): 739. http://dx.doi.org/10.3390/pr8060739.

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Heterotrophic bacteria are able to form biofilms in water processing systems, adhering to pipe materials and colonizing surfaces. The aim of our research was to identify the critical points in the process of bottled water production at which controls can be applied to prevent, reduce, or eliminate water safety hazards. Microbiological monitoring was conducted using the plate count method and luminometry. To identify the bacterial isolates, we used polyphasic identification based on biochemical tests and molecular analysis using ribosomal RNA. The heterotrophic plate counts were higher in the w
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O'Toole, George A. "Classic Spotlight: Plate Counting You Can Count On." Journal of Bacteriology 198, no. 23 (2016): 3127. http://dx.doi.org/10.1128/jb.00711-16.

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Reasoner, Donald J. "Heterotrophic plate count methodology in the United States." International Journal of Food Microbiology 92, no. 3 (2004): 307–15. http://dx.doi.org/10.1016/j.ijfoodmicro.2003.08.008.

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27

CHAMPAGNE, CLAUDE P., NANCY J. GARDNER, JULIE FONTAINE, and JACQUES RICHARD. "Determination of Viable Bacterial Populations in Raw Milk within 20 Minutes by Using a Direct Epifluorescent Filter Technique." Journal of Food Protection 60, no. 7 (1997): 874–76. http://dx.doi.org/10.4315/0362-028x-60.7.874.

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The results from a shortened procedure for the direct epifluorescent filter technique (DEFT) determination of viable bacterial populations in raw milk were compared to standard plate counts. Shortening the prefiltration trypsin-Triton X-100 incubation period from 10 to 3 min enabled the completion of the analysis within 20 min. The short DEFT method results had a correlation coefficient (r) of 0.81 with plate counts. With respect to precision, the average difference between values of duplicate plate count analyses was 0.16 log units; that of the short DEFT was 0.14 log units. The slopes of the
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ENTIS, PHYLLIS, and PETER BOLESZCZUK. "Use of Fast Green FCF with Tryptic Soy Agar for Aerobic Plate Count by the Hydrophobic Grid Membrane Filter." Journal of Food Protection 49, no. 4 (1986): 278–79. http://dx.doi.org/10.4315/0362-028x-49.4.278.

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A hydrophobic grid membrane filter (HGMF) method for aerobic plate count using Tryptic Soy Agar with fast green FCF was evaluated against a conventional pour plate method on 250 food samples, representing 25 product categories. The HGMF method yielded counts equivalent to or significantly higher than the pour plate method for 24 of the 25 product categories (t-test for paired data).
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PARK, YONG HO, KEUN SEOK SEO, JONG SAM AHN, HAN SANG YOO, and SANG PIL KIM. "Evaluation of the Petrifilm Plate Method for the Enumeration of Aerobic Microorganisms and Coliforms in Retailed Meat Samples." Journal of Food Protection 64, no. 11 (2001): 1841–43. http://dx.doi.org/10.4315/0362-028x-64.11.1841.

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This study was designed to compare the effectiveness and applicability of the Petrifilm plate method with the Association of Official Analytical Chemists' (AOAC) standard aerobic count method and violet red bile agar method for meat products. The comparison was carried out using 303 meat samples collected from various retailers: 110 pork samples, 87 chicken samples, and 107 beef samples. In the comparison of the correlation coefficient (R) between the conventional method and the Petrifilm plate method by a linear regression analysis, the correlation coefficient in total microorganisms was 0.99
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Janssen, Peter H., Penelope S. Yates, Bronwyn E. Grinton, Paul M. Taylor, and Michelle Sait. "Improved Culturability of Soil Bacteria and Isolation in Pure Culture of Novel Members of the Divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia." Applied and Environmental Microbiology 68, no. 5 (2002): 2391–96. http://dx.doi.org/10.1128/aem.68.5.2391-2396.2002.

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ABSTRACT The culturability of bacteria in the bulk soil of an Australian pasture was investigated by using nutrient broth at 1/100 of its normal concentration (dilute nutrient broth [DNB]) as the growth medium. Three-tube most-probable-number serial dilution culture resulted in a mean viable count that was only 1.4% of the mean microscopically determined total cell count. Plate counts with DNB solidified with agar and with gellan gum resulted in viable counts that were 5.2 and 7.5% of the mean microscopically determined total cell count, respectively. Prior homogenization of the soil sample wi
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RACHMAWATI, NOOR AFIFAH, SURANTO SURANTO, and SOLICHATUN SOLICHATUN. "The effect of drying methods variation on saponin content, total plate count, and pathogen bacteria of simplisia of Sesbania grandiflora (L.) Pers. leaf extract." Biofarmasi Journal of Natural Product Biochemistry 4, no. 1 (2006): 4–9. http://dx.doi.org/10.13057/biofar/f040102.

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The purposes of this research were to study the effect of drying methods variation on saponin content, total plate count, and pathogen bacteria of simplicia of Sesbania grandiflora (L.) Pers. leaf extract. The research was conducted by using Completely Random Design with the single factor and three replications. The treatment consisted of four treatments: without drying sample (as control) (P0), direct sunray drying (P1), indirect sunray (under shade place) drying (P2), and vacuum drying oven at temperature 45oC (P3). The observed parameters were: saponin content, total plate count, and pathog
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Bird, Patrick, Jonathan Flannery, Erin Crowley, et al. "Evaluation of the 3M™ Petrifilm™ Rapid Aerobic Count Plate for the Enumeration of Aerobic Bacteria: Collaborative Study, First Action 2015.13." Journal of AOAC INTERNATIONAL 99, no. 3 (2016): 664–75. http://dx.doi.org/10.5740/jaoacint.15-0260.

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Abstract The 3M™ Petrifilm™ Rapid Aerobic Count (RAC) Plate is a sample-ready culture medium system containing dual-sensor indicator technology for the rapid quantification of aerobic bacteria in food products. The 3M Petrifilm RAC Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual (FDA BAM) Chapter 3 (Aerobic Plate Count) for the enumeration of aerobic bacteria in raw easy-peel shrimp and the Standard Methods for the Examination of Dairy Products (SMEDP) Chapter 6 (Standard Plate Count Method) for the enumeration of aerobic bacteria in pasteurized sk
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Crowley, Erin S., Patrick M. Bird, Marianne K. Torontali, et al. "TEMPO® TVC for the Enumeration of Aerobic Mesophilic Flora in Foods: Collaborative Study." Journal of AOAC INTERNATIONAL 92, no. 1 (2009): 165–74. http://dx.doi.org/10.1093/jaoac/92.1.165.

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Abstract The automated system for enumeration of total viable count (TVC) in foods, TEMPO® TVC, uses a dehydrated culture medium and an enumeration card containing 48 wells across 3 different dilutions for the automatic determination of the most probable number (MPN). The alternative method was compared in a multilaboratory collaborative study to AOAC Method 966.23 for determination of aerobic plate count for nondairy products and the Standard Methods for the Examination of Dairy Products (SMEDP) Standard Plate Count for dairy products. Five food types, raw ground beef, raw ground chicken, coo
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KHAYAT, F. A., J. C. BRUHN, and G. H. RICHARDSON. "A Survey of Coliforms and Staphylococcus aureus in Cheese Using Impedimetric and Plate Count Methods1." Journal of Food Protection 51, no. 1 (1988): 53–55. http://dx.doi.org/10.4315/0362-028x-51.1.53.

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A total of 256 cheese samples were analyzed for coliform plate count using violet red bile agar and for an impedance count using BactometerR Coliform Medium with a correlation coefficient between methods of R=−.91. Fifty-four percent of the samples contained 102 to 107 colony forming units/gram (CFU/g). The highest counts were in cream and fresh cheese products. When 27 Cheddar cheese samples were inoculated with from 102 to 107 CFU of Escherichia coli/g a correlation of R=−.97 was found between methods. Two hundred of the cheese samples were analyzed for Staphylococcus aureus using Baird-Park
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35

Tremsin, A. S., J. F. Pearson, G. W. Fraser, W. B. Feller, and P. White. "Microchannel plate operation at high count rates: new results." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 379, no. 1 (1996): 139–51. http://dx.doi.org/10.1016/0168-9002(96)00482-2.

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LeChevallier, M. W., and G. A. McFeters. "Interactions between heterotrophic plate count bacteria and coliform organisms." Applied and Environmental Microbiology 49, no. 5 (1985): 1338–41. http://dx.doi.org/10.1128/aem.49.5.1338-1341.1985.

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37

Potera, Carol. "Siderophores Shed Light on the “Great Plate Count Anomaly”." Microbe Magazine 5, no. 8 (2010): 325–26. http://dx.doi.org/10.1128/microbe.5.325.1.

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Bartram, J., J. Cotruvo, M. Exner, C. Fricker, and Axel Glasmacher. "Heterotrophic plate count measurement in drinking water safety management." International Journal of Food Microbiology 92, no. 3 (2004): 241–47. http://dx.doi.org/10.1016/j.ijfoodmicro.2003.08.005.

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39

Fraser, G. W., M. T. Pain, and J. E. Lees. "Microchannel plate operation at high count rates: further studies." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 327, no. 2-3 (1993): 328–36. http://dx.doi.org/10.1016/0168-9002(93)90698-h.

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40

GOLDBERG, JOHN J., JOSEPH W. PANKEY, PEGGY A. DRECHSLER, PATRICIA A. MURDOUGH, and DIANTHA B. HOWARD. "An Update Survey of Bulk Tank Milk Quality in Vermont." Journal of Food Protection 54, no. 7 (1991): 549–53. http://dx.doi.org/10.4315/0362-028x-54.7.549.

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Quality of Vermont bulk tank milk was first surveyed in 1985 as part of a statewide milk quality enhancement program. In a second survey conducted in 1990, bulk tank milk from 1,971 farms was sampled and tested for standard plate count, bacterial type and species distribution, and somatic cell count. Test results from 1,203 duplicate bulk tank milk samples were compared between five Vermont milk processors and the University of Vermont Quality Milk Research Laboratory. Arithmetic mean standard plate count conducted by processors was 2.3 × 104 CFU/ml in 1990 compared with 3.0 × 104 CFU/ml in 19
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Nelson, Maria T., Robert A. LaBudde, Stephen F. Tomasino, et al. "Comparison of 3M™ Petrifilm™ Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study." Journal of AOAC INTERNATIONAL 96, no. 4 (2013): 717–22. http://dx.doi.org/10.5740/jaoacint.12-469.

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Abstract A multilaboratory study was conducted to determine the equivalence of the 3M™ Petrifilm™ Aerobic Count Plate and standard plating methodology for measuring viable bacteria and spores recovered from hard-surface carriers (stainless steel and porcelain), also known as "control carrier counts," used in AOAC antimicrobial efficacy test methods. Six laboratories participated in the study in which carriers inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, and spores of Bacillus subtilis were evaluated using 3M Petrifilm Aerobic Count (AC) plates and standar
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Ortolani, Maria BT, Gabriela N. Viçosa, Vanerli Beloti, and Luís A. Nero. "Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm™ Aerobic Count plates and conventional pour plate methodology." Journal of Dairy Research 74, no. 4 (2007): 387–91. http://dx.doi.org/10.1017/s002202990700266x.

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This study aimed to compare Petrifilm™ Aerobic Count (AC) plates and the conventional pour plate methodology using de Mann-Rogosa-Sharpe (MRS), Kang-Fung (KF) and Kang-Fung-Sol (KFS) culture media for screening and enumeration of lactic acid bacteria (LAB) in milk. Suspensions of 10 LAB species in reconstituted powder skim milk and 30 raw milk samples, without experimental inoculation, were tested. For selective enumeration, all samples were previously diluted in MRS, KF and KFS broths and then plated in Petrifilm™ AC and conventional pour plate methodology, using the same culture media with a
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Kartika Pratiwi, I. Desak Putu, I. Ketut Suter, Putu Ari Sandhi Widpradnyadewi, and Anak Agung Istri Sri Wiadnyani. "Perubahan Fisiko-Kimiawi dan Mikrobiologis Minuman Tradisional Bali (Loloh) selama Penyimpanan." agriTECH 39, no. 1 (2019): 70. http://dx.doi.org/10.22146/agritech.17261.

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Loloh is a Balinese traditional beverage made from one or a combination of several herbal extracts. The most popular variants of loloh in Bali are loloh tibah and loloh cem-cem, which are continuously produced every day. During distribution, loloh is stored at room temperature. The study was aimed to study the physico-chemical and microbiological characteristics of loloh cem-cem and loloh tibah during storage at room temperature. This study used a purposive random sampling. The samples consisted of 14 loloh sellers in Badung-Denpasar. Type parameters that were observed during a 24 hour-storage
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JAY, J. M. "A Review of Aerobic and Psychrotrophic Plate Count Procedures for Fresh Meat and Poultry Products." Journal of Food Protection 65, no. 7 (2002): 1200–1206. http://dx.doi.org/10.4315/0362-028x-65.7.1200.

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This is a review of reports that employed aerobic plate counts on fresh meat and poultry products since 1985; it lists synopses of 100 applications. A total of 15 different plating media were used, with 48 (48%) being either plate count agar (PCA) or tryptone glucose yeast extract agar. The temperature-time relations ranged from a low temperature of 20°C for 120 h to 37°C for 24 h. Some 29 different temperature-time combinations were used among the total of 109, with 21 (19.3%) being 35°C/48 h, followed by 12 (11.0%) at 32°C/48 h, 11 (10.1%) at 25°C/48 h, and 9 (8.3%) at 25°C/72 h. Fifty-four
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BEUCHAT, L. R., F. COPELAND, M. S. CURIALE, et al. "Comparison of the SimPlate™ Total Plate Count Method with Petrifilm™, Redigel™, and Conventional Pour-Plate Methods for Enumerating Aerobic Microorganisms in Foods." Journal of Food Protection 61, no. 1 (1998): 14–18. http://dx.doi.org/10.4315/0362-028x-61.1.14.

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The SimPlate™ Total Plate Count (TPC) method, developed by IDEXX Laboratories, Inc., is designed to determine the most probable number of aerobic microorganisms in foods. The 24-h test was compared to the conventional plate count agar (PCA) method, the Petrifilm™ Aerobic Count plates, and the Redigel™ Total Count procedure for enumerating microflora in 751 food samples. Results using the SimPlate™ TPC method were highly correlated (r ≥ 0.96) with results from other test methods. Slopes (0.96–0.97) were not significantly different from 1, and y intercepts (−0.03–0.08) were not different from 0.
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Mutiarasari, Nonie Olivia Adia, Nenny Harijani, Fedik Abdul Rantam, Dadik Raharjo, Agnes Theresia Soelih Estoepangestie, and Didik Handijatno. "Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya." Journal of Basic Medical Veterinary 9, no. 2 (2021): 63. http://dx.doi.org/10.20473/jbmv.v9i2.28584.

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This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identificatio
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Schade, M., and H. Lemmer. "Counting Bacteria of Selected Metabolic Groups in Activated Sludge – An Assessment of Methods." Water Science and Technology 29, no. 7 (1994): 75–79. http://dx.doi.org/10.2166/wst.1994.0312.

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For counting bacteria of selected metabolic groups in activated sludge several methods such as the MPN-method, the pour plate method, the surface plate method, and the membrane filter technique are available. Population densities of heterotrophic saprophytes were assessed with the MPN-method, the pour plate and the surface plate method. The surface plate method yielded higher bacterial counts compared to the pour plate method. The MPN-method is not suitable because of the ambiguity of results leading to large statistical errors. The membrane filter technique yielded significantly higher counts
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48

Gibbs, R. A., J. E. Scutt, and B. T. Croll. "Assimilable Organic Carbon Concentrations and Bacterial Numbers in a Water Distribution System." Water Science and Technology 27, no. 3-4 (1993): 159–66. http://dx.doi.org/10.2166/wst.1993.0340.

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A three year study was conducted to investigate bacterial growth in a drinking water distribution system in the UK. Bacterial numbers were estimated using Yeast Extract Agar plate counts. Plate counts in the distribution system showed patterns of spatial and seasonal variation. The spatial pattern was that plate counts increased through the distribution system until approximately 30 to 40 hours retention time and remained constant further through the distribution system. The seasonal pattern was that plate counts were low in the winter and had large peaks in the summer and autumn. Assimi able
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RIVAS, TERESA, JUAN A. VIZCAÍNO, and FRANCISCO J. HERRERA. "Microbial Contamination of Carcasses and Equipment from an Iberian Pig Slaughterhouse." Journal of Food Protection 63, no. 12 (2000): 1670–75. http://dx.doi.org/10.4315/0362-028x-63.12.1670.

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The microbial contamination of carcasses and equipment has been studied in an industrial slaughterhouse of Iberian pigs. Samples of the surface of carcasses were taken at different stages of the process and aerobic plate count at 37°C (APC), Enterobacteriaceae-count (E-count) and Escherichia coli-count (EC-count) were determined. It was demonstrated that in scalding and singeing the APC decreased (P < 0.01), while in the dehairing it increased (P < 0.01). The E-count and EC-count decreased in the scalding but increased in the evisceration (P < 0.001). The implementation of
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Reichert-Schwillinsky, Franziska, Carmen Pin, Monika Dzieciol, Martin Wagner, and Ingeborg Hein. "Stress- and Growth Rate-Related Differences between Plate Count and Real-Time PCR Data during Growth of Listeria monocytogenes." Applied and Environmental Microbiology 75, no. 7 (2009): 2132–38. http://dx.doi.org/10.1128/aem.01796-08.

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ABSTRACT To assess the overestimation of bacterial cell counts in real-time PCR in relation to stress and growth phase, four different strains of L. monocytogenes were exposed to combinations of osmotic stress (0.5 to 8% [vol/vol] NaCl) and acid stress (pH 5 to 7) in a culture model at a growth temperature of 10°C or were grown under optimal conditions. Growth curves obtained from real-time PCR, optical density, and viable count data were compared. As expected, optical density data revealed entirely different growth curves. Good to moderate growth conditions yielded good correlation of real-ti
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