Dissertations / Theses: 'Platelet-rich fibrin'

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Peck, M. Thabit. "The effect of storage time on the platelet concentration of Choukroun's platelet rich fibrin (PRF)." University of the Western Cape, 2011. http://hdl.handle.net/11394/5219.

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Magister Chirurgiae Dentium (MChD)
Wound healing is a complex process characterised by the repair and reconstitution of lost or damaged tissue. By the mid 1990s, several methods were proposed to enhance wound healing of surgical sites by introducing high concentrations of human platelets to these areas. In the early 21st century, Choukroun et al (2006b) introduced a new type of platelet concentrate that was devoid of any additives, and required no specialised equipment for its production. This concentrate was termed Platelet-rich fibrin (PRF) and although various aspects of this biomaterial had been studied, very little is currently known about its storage properties. Aim: To determine whether storage time had a significant effect on the platelet concentration of Choukroun’s PRF Method: A total of 30 patients were enrolled into the study. Three blood samples of 10ml each were drawn from each patient. Two of the blood samples (Group A and Group B) were centrifuged to form PRF. The third sample was used to measure the baseline blood platelet concentration and was therefore not centrifuged. After PRF had formed in both test groups, it was removed from the test tubes at 2 different times i.e. immediately after centrifuge (Group A) or after 60 min of storage in the blood collecting tube (Group B). The remaining blood was then tested for platelet concentration and compared to each other and the baseline reading. Results: 14 males and 16 females participated in the study (average age 41.7 years). A mean blood platelet concentration of 282.8 ± 58.3 × 109/L was recorded for the baseline reading. Group A had a mean blood platelet concentration 7.9 ± 3.03 × 109/L. Group B had a mean blood platelet concentration of 4.0 ± 1.93 × 109/L. A statistically significant difference was seen between Groups A and B (p < 0.0001). Conclusions: Storage time has a significant effect of the platelet concentration of PRF. If stored over a period of 60 min, the platelet concentration of PRF increases. Further research is required to determine whether this finding is clinically significant.

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Dohan, David. "Platelet Rich Fibrin (PRF) : modèle théorique et études préliminaires." Paris 5, 2005. http://www.theses.fr/2005PA05M004.

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Le PRF (Platelet Rich Fibrin) est un adjuvant chirurgical issu des technologies des colles de fibrine et des concentrés plaquettaires autologues de première génération (les PRP). L'objectif de ce travail est de réaliser une synthèse historique et technologique de cette famille d'adjuvants chirurgicaux à base de fibrine, de tenter de déterminer un premier modèle théorique de l'architecture moléculaire du PRF et enfin, à l'aide d'observations cliniques et d'analyses histologiques, d'évaluer le potentiel cicatriciel du PRF au niveau des tissus mous ainsi qu'au cours de la maturation des greffes osseuses
PRF (Platelet Rich Fibrin) is a surgical additive coming from the autologous fibrin adhesives and first generation platelet concentrates (PRP) technologies. The objective of this work is to make a historical and technological synthesis of this family of fibrin related surgical additives, to create a first theoretical model of the PRF molecular architecture and finally, with clinical observations and histological analysis, to evaluate the healing potential of the PRF in soft tissue wounds and during bone graft maturation

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Hasan, Fadi K. "Characterization of Leukocyte-Platelet Rich Fibrin, a Novel Biomaterial." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3749.

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Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and are successfully used in oral surgery. Several commercial systems exist that generate various forms of platelet concentrates including Platelet-rich plasma (PRP) and Platelet-rich fibrin (PRF). The alpha- granules of entrapped platelets release a variety of peptide growth factors that promotes healing. Usually PRP is a suspension that can be injected into the site of injury or used as a gel with the addition of thrombin (PRP-gel). In contrast Choukroun’s L-PRF is a dense fibrin based biomaterial enriched with platelets and growth factors. The physical state of these natural biomaterials especially L-PRF permits manual handling and suturing onto the tissue bead to improve healing. However, our knowledge about the mechanical characteristic of L-PRF is quite limited and a good understanding of material properties will enable expansion of current clinical applications. This study demonstrates the techniques to identify L-PRF’s mechanical properties (uniaxial tensile testing and suture retention strength); morphology (scanning electron microscope); biological stability and cytocompatibility.

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Barona, Intriago Maria Fernanda. "Regulation of gingival fibroblast gene expression by leukocyte and platelet rich fibrin." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62647.

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Objectives: Leukocyte and Platelet-Rich Fibrin (L-PRF), a new clinically used blood product, is rich in leukocytes and platelets embedded in a high-density fibrin network. L-PRF has shown some clinical promise to promote gingival wound healing. In gingival surgeries where L-PRF is being used, it will be in direct contact with human gingival fibroblasts (HGFs) of the surgical flap. However, little is known about the combined effects of the growth factors and cells present in L-PRF on HGFs. We hypothesized that L-PRF regulates expression of key wound healing genes in HGFs. Thus, our aim was to investigate the effects of soluble biological factors released from L-PRF on HGF gene expression. Methods: L-PRF membranes were produced from blood samples of six volunteer donors using standard clinical protocol. The membranes were placed into upper compartments of transwell plate inserts (0.4 µm pore size) with confluent layers of HGFs at the bottom of the wells. Thus, only soluble mediators were able to influence HGF gene expression. HGFs with the L-PRF membranes in the wells were incubated for 48h followed by isolation of total RNA for real-time quantitative PCR of wound healing related genes. The levels of selected proteins produced by the cells were analyzed using Western blotting. Results: Among the 86 genes studied, the expression of 35 genes (41%) was significantly regulated by the L-PRF membranes in HGFs. Replicate membranes from the same donor or between different donors produced similar responses, with membranes from different individuals varying somewhat in magnitude of the response. Among the most regulated genes, matrix metalloproteinase-1 (MMP-1) and MMP-3 showed several-fold increase by L-PRF-treatment, both at the mRNA and protein level. Angiogenesis related genes, VEGF-α and FGF-2 showed also significant up-regulation in HGFs by the L-PRF-treatment. Conclusions: The results demonstrate that the L-PRF membranes have a strong and a specific regulatory effect on HGFs that may modulate several aspects of wound healing.
Dentistry, Faculty of
Graduate

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Peck, Mogammad Thabit. "An analysis of the morphological and biological properties of a novel human leukocyte- and platelet- rich concentrate." University of the Western Cape, 2018. http://hdl.handle.net/11394/6841.

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Philosophiae Doctor - PhD
Wound healing is a complex process that involves several overlapping and interacting biological pathways. The consequences of delayed or abnormal wound healing may result in tissue formation that has impaired function or structural abnormalities. As a result, clinicians have sought ways to enhance this process. Recently, the use of autologous platelet concentrates have become popular in the management of wound healing sites. However, controversy exists as to how these biomaterials should be prepared and applied. We therefore sought to investigate whether a biologically viable and clinically effective platelet concentrate could be prepared using standard laboratory equipment. The findings are presented in a series of articles that have been published in peer-reviewed journals. The results suggest that the experimental platelet concentrate produced, has a morphological structure that consists of a dense fibrin network intermingled with platelets, has the ability to accelerate cellular growth in-vitro, has no adverse effects on cells in-vitro, can concentrate and release a systemically ingested antibiotic over a period of 24 hours in-vitro, can be stored for at least 60 minutes without showing signs of deterioration, and has shown clinical evidence of accelerating wound healing in sinus augmentation and alveolar ridge preservation procedures. The reduced cost of producing such a biomaterial allows it to be available to resource poor settings and to wider range of healthcare providers as compared to standard platelet concentration techniques. Further studies are required to investigate the clinical potential of this promising biomaterial.

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Bhamjee, Feheem. "The in vitro antimicrobial activity of advanced platelet rich fibrin (A-PRF) against microorganisms of the oral cavity." University of the Western Cape, 2017. http://hdl.handle.net/11394/6232.

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Magister Chirurgiae Dentium - MChD (Oral Medicine and Periodontics)
In recent years, the development and use of autologous platelet rich concentrates (PC's) has gained traction within the rapidly progressive, multidisciplinary field of regenerative medicine. A PC subtype, marketed as advanced platelet rich fibrin (A- PRF), is a recent advancement of the original PRF protocol and promoted as a "blood concentrate" containing platelets, leukocytes, circulating stem cells and endothelial cells. A-PRF in the form of membranes, plugs, or even shredded particulates are increasingly being used as surgical adjuncts in areas of previous infection or left exposed within the microbial rich oral environment. Although recent literature has noted the biologic benefits of this material within the context of wound healing and regeneration, the antimicrobial potential of APRF has remained unexplored. The aim of this investigation is to determine if A-PRF displays antimicrobial activity against microbes of the oral cavity with a null hypothesis that its activity is no different to a clot of unprocessed venous blood. Methodology: A-PRF and whole blood samples were obtained from consenting individuals and utilised to conduct an in-vitro agar disk diffusion investigation to determine their antimicrobial activity. Standardised samples of A-PRF, unprocessed clotted blood and 0.2% chlorhexidine gluconate (CHX) were tested against organisms cultured from fresh oral rinse samples and pure cultures of candida albicans, streptococcus mutans, staphylococcus aureus and enterococcus faecalis. The antimicrobial activity was assessed in accordance to the established principles of the agar disk diffusion method and measurement of inhibition zones. Results: A-PRF displayed antimicrobial activity against all of the individual organisms tested within this study following a 24 hour incubation period. However, no significant differences were noted between A-PRF and a natural clot of blood when tested against cultures of the oral rinse sample. Finally, the antimicrobial activity of A-PRF is significantly inferior to an equal volume of the CHX preparation. Conclusion: Although A-PRF displays antimicrobial activity; its strength, spectrum and biologic activity within a polymicrobial environment requires further investigation.

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Zhu, Christina Zhen Chao. "The effect of leucocyte and platelet-rich fibrin (L-PRF) on healing of bone defects in dogs." Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/18851.

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Objective: To evaluate and compare healing between L-PRF, cancellous bone graft(BG) and L-PRF and BG(BOTH) on the gap created during tibial tuberosity advancement procedure(TTA). Study Design: Prospective clinical study involving dogs treated with TTA (n=36). Hypothesis: The first hypothesis was that L-PRF could be used in dog long bones without causing clinically evident complications. The second hypothesis was that bone defects filled with BOTH would heal faster than those filled with BG alone. Material and Methods: Dogs underwent TTA at the University Veterinary Teaching Hospital Sydney via a technique using a tension-band plate. Ethics approval was obtained from the USYD AEC approval number 2013/5993. Consent was established prior to study inclusion however owners were blinded to treatment group (TG). TGs had osteotomy filled with L-PRF, BG or BOTH. A total of 36 stifles from 35 dogs met the inclusion criteria. Post-op radiographs from 2, 4 and 6weeks were graded using a 0-4 point scale. Computed tomography(CT) was also performed at 6weeks to assess bone healing. Logistic regression analysis was done to look at assessment parameters and the effect of TGs on osteotomy healing. Results: There was no effect of TG or time on rate of complications. There was no statistical significance between BG and BOTH groups except the proximal healing for the BOTH group was 2.49 times more likely to have a higher score than BG. BOTH scored significantly higher than L-PRF except for proximal healing (no difference). There was no effect of differing radiologists assessment on osteotomy fill, or TG on the mean Hounsfield Units at location ½ and ⅓ of the way distal to the cage. Conclusion: This study has demonstrated that L-PRF is a viable product for TTA resulting in successful bone healing. Healing at the proximal site using BG is the same as L-PRF based on radiographs. CT analysis suggests that the effect of L-PRF on bone healing is comparable to BG at 6 weeks post TTA.

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Glazier, Thomas F., Thomas C. Waldrop, John C. Gunsolley, and Robert Sabatini. "The Role of Leukocyte-Platelet Rich Fibrin in Human Alveolar Ridge Preservation: A Randomized Clinical Trial." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3735.

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PURPOSE: The aim of this study is to examine the healing of intact extraction sockets grafted with leukocyte-platelet rich fibrin (L-PRF) as compared to sockets grafted with freeze-dried bone allograft (FDBA) and a resorbable collagen barrier membrane (RCM). METHODS: This prospective randomized, examiner blinded pilot study included 17 subjects randomized to two treatment groups. Serum total cholesterol, low density lipoprotein (LDL), 25-hydroxyvitamin-D3, and platelet counts were determined preoperatively in all subjects. The experimental arm consisted of 8 posterior tooth-bounded intact extraction sites receiving L-PRF plugs. The control group consisted of 9 posterior tooth-bounded intact extraction sites receiving FDBA and RCM. An acrylic stent was fabricated to take duplicate clinical and CBCT measurements immediately post-extraction and at time of implant placement. A repeat-measures analysis of variance was utilized for statistical analysis. RESULTS: The study failed to detect a clinical or radiographic difference between treatment groups in horizontal or vertical ridge dimension changes. Serum cholesterol, LDL, 25-hydroxyvitamin-D3, and buccal plate thickness had a non-significant effect on outcome measurements, although there was a high prevalence of hyperlidpidemia and hypovitaminosis in the study population. CONCLUSIONS: The alveolar ridge dimension changes in intact posterior extraction sockets may be similar when either L-PRF or FDBA and RCM are utilized as socket grafting material. Although there was a high prevalence of high cholesterol and low 25-hydroxyvitamin-D3 levels in the population, this study failed to detect a significant correlation between preoperative serum levels and postoperative ridge dimension changes.

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MURGIA, Denise. "NEW GUIDED BONE REGENERATION PROCEDURE USING LEUKOCYTE AND PLATELET RICH FIBRIN (L PRF) IN ORAL SURGERY." Doctoral thesis, Università degli Studi di Palermo, 2021. http://hdl.handle.net/10447/500658.

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Non-transfusional hemocomponents are autogenous products used in several surgical fields obtained by the centrifugation of a blood sample from a patient and able to promote hard and soft tissue regeneration, local haemostasis, and the acceleration of wound healing. The aim of this PhD thesis was the validation of an innovative protocol for the Guided Bone Regeneration (GBR) technique using second-generation autologous platelet concentrates, Leukocytes and Platelet-rich Fibrin (L-PRF), in post-extraction sockets of patients in need of dental avulsions and successive implant prosthetic rehabilitation, evaluating its ability to prevent alveolar bone resorption and promote bone regeneration. The secondary aim was the design and development of a multicomposite loaded with antimicrobic and antioxidant agents, to be applied wrapped in the L-PRF membrane, enhancing its regenerative properties. This project cohering with the National Operational Program (PON) “Research and Innovation” (R&I) 2014-2020 funding by the Italian Ministry of Education, University and Research, aimed to promote the research and the innovation of the country, with a particular interest on the Health Specialization Area.

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Schuldt, Luisa Antonia. "In vitro evaluation of the effects of leukocyte-platelet rich fibrin on disinfection of a rough implant surface." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62613.

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Objectives: Peri-implantitis is a frequent and serious clinical problem affecting between 1 and 47% of implants. Bacterial contamination of the roughened implant surface plays a major role in the etiology and progression of the disease. Successful treatment of peri-implantitis requires disinfection of the rough implant surface. There is no generally accepted protocol for implant disinfection. Autologous leukocyte and platelet-rich fibrin (L-PRF) membranes can be produced from autologous human blood via a one-step centrifugation procedure. It was hypothesized that the antimicrobial defense system of L-PRF may decontaminate the SLAⓇ implant surface. The objective of this study was to test the efficacy of L-PRF for SLAⓇ implant surface disinfection. Methods: Collagen-coated SLAⓇ (sand blasted, large grit acid etched) titanium discs were inoculated with dispersed dental plaque with a minimum bacterial cell concentration of 3.2 × 10⁷ CFU/ml. After 21 days of anaerobic incubation at 37C, discs were rinsed with 12 ml 0.9% NaCl to remove unattached biofilm, and exposed for 48 hours to Leukocyte-Platelet Rich Fibrin (L-PRF) in DMEM. Disks with or without rinsing with 12 ml of 0.9 % NaCl were fixed for SEM. Bacterial counts and perforations in bacteria were quantified from standardized scanning electron micrographs of the implant surface. The rinsing solution was collected and Western blot analysis was performed. L-PRF disks were compared with the control group (rinse). Results: Difference in presence of bacteria displaying perforation of the cell wall between cell–rich L-PRF treated samples and rinsed control group was statistically significant (p < 0.0001, Fisher's Exact Test). Western blot analysis of the rinse fluid demonstrated presence of Platelet Factor-4. Activated platelets in intimate contact with bacteria were detected on SEM images. SEM analysis demonstrated a statistically significant reduction of residual bacteria in the lacunae of the rough SLAⓇ surface after L-PRF treatment. (p< 0.05, Kruskal-Wallis). Conclusions: Autologous L-PRF may have potential as a biological means to decontaminate rough implant surfaces, possibly by exploiting the antimicrobial effects of platelets.
Dentistry, Faculty of
Graduate

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McCormack, Danielle M. "Profiling Precursor Lipids for Specialized Pro-Resolution Molecules in Platelet-Rich Fibrin Following Fish Oil and Aspirin Intake." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4761.

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Background: Current research has demonstrated that aspirin and fish oil (EFA) increase plasma levels of specialized pro-resolution molecules (SPMs). This study investigates their effects on SPM precursor pools in platelet rich fibrin (PRF). Methods: Twenty healthy volunteers were randomly assigned to take aspirin; EFA or aspirin and EFA. Four hours later, SPM precursor levels were quantified using combined Liquid Chromatography tandem mass spectrometry. The differences between the groups: Aspirin (yes or no), EFA (yes or no), were analyzed by ANCOVA, testing for group differences after covarying out the baseline value. Results: There were 4 significant interactions, 1 with an aspirin effect, 2 with an EFA effect, and 64 with no difference between the groups. The significant interaction effect was found for the following lipidome: LPE(20:4), LPI(16:1), LPI(18:1), and LPI(20:3). Aspirin decreased the LPG(16:4) levels, and EFA decreased the LPE(22:5) and PG(16:0/18:0) lipidomes. Conclusions: Some SPM precursor pools in PRF were increased following supplementation.

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Resende, Margarida Reynolds de Sousa. "Utilização de laser ou de fibrina rica em plaquetas no maneio de feridas do membro distal equino." Master's thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2019. http://hdl.handle.net/10400.5/18819.

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Dissertação de Mestrado Integrado em Medicina Veterinária
As feridas do membro distal equino que cicatrizam por segunda intenção, são potencialmente de cicatrização lenta. O objetivo do estudo consistiu na avaliação do efeito terapêutico do laser ou da aplicação de fibrina rica em plaquetas (PRF) na cicatrização de feridas por segunda intenção de membros distais de equinos e de etiologia traumática, em comparação com o método convencional na prática clínica. Todas as feridas (n=9) possuíram diferentes dimensões iniciais e tempos desde a sua lesão até à primeira observação clínica. Consideraram-se três grupos: o grupo controlo (pomada tópica + penso); o grupo laser (terapia do laser + penso); e o grupo PRF (PRF + penso). As feridas foram fotografadas e medidas, com posterior cálculo das suas áreas diárias (retangular e elítica) e da sua taxa de cicatrização. Na análise estatística utilizou-se o teste de variância unifatorial (one-Way ANOVA) e o teste post-hoc de Duncan, com significância de P<0,05. A taxa de cicatrização foi superior no grupo laser em relação aos grupos controlo e PRF (P<0,05), mas semelhante entre o grupo PRF e o grupo controlo. Em conclusão, este estudo preliminar sugere a continuação de trabalhos de estudo em feridas do membro distal do cavalo utilizando laser e PRF numa maior amostragem e em condições controladas.
ABSTRACT - LASER OR PLATELET-RICH FIBRIN APPLICATION ON MANAGEMENT OF EQUINE DISTAL LIMB WOUNDS - Equine distal limb wounds that are left to heal by second intention are potentially slow healing. This study evaluated the therapeutic effect of laser or the application of platelet-rich fibrin (PRF) in wound healing by second intention of distal limbs of horses and of traumatic etiology, in comparison with the conventional method in clinical practice. All wounds (n = 9) had different initial dimensions and times since their injury until their first clinical observation. Three groups were considered: the control group (topical ointment + dressing); laser group (laser therapy + dressing); and the PRF group (PRF therapy + dressing). The wounds were photographed and measured, with subsequent calculation of their daily areas (rectangular and elliptical) and their healing rate. In the statistical analysis, a one-way ANOVA and a post hoc comparison test (Duncan test) were used, with significance of P < 0.05. The healing rate was higher in the laser group compared to the control and PRF groups (P < 0.05), but similar between the PRF group and the control group. In conclusion, this preliminary study suggests the continuation of studies on wounds of the distal limb of the horse using laser and PRF therapies in a larger sampling and under controlled conditions.
N/A

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Malzoni, Carolina Mendonça de Almeida. "Utilização da fibrina rica em plaquetas e leucócitos (L-PRF) em procedimentos cirúrgicos de elevação do assoalho do seio maxilar /." Araraquara, 2020. http://hdl.handle.net/11449/192532.

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Orientador: Daniela Leal Zandim-Barcelos
Resumo: A fibrina rica em plaquetas e leucócitos (L-PRF) é um derivado plaquetário autógeno capaz de liberar citocinas e fatores de crescimento favoráveis ao reparo tecidual. Por esta razão a L-PRF vem sendo utilizada em diversos procedimentos odontológicos. Uma das aplicabilidades clínicas da L-PRF é em procedimentos de levantamento do seio maxilar, uma técnica já consagrada na odontologia que permite a colocação de implantes dentários em região posterior de maxilas atróficas. Para avaliar a efetividade do uso da L-PRF, dois estudos clínicos independentes foram realizados utilizando a L-PRF em seios maxilares. Um deles teve o propósito de avaliar a influência da L-PRF na regeneração óssea quando associada ao osso mineral bovino desproteinizado (OBD) em procedimentos de elevação do assoalho do seio maxilar, e o outro teve o propósito de avaliar a efetividade da L-PRF no reparo de membranas de Schneider acidentalmente perfuradas durante o procedimento de levantamento do seio maxilar. Para isso, o primeiro trabalho foi um estudo clínico randomizado controlado envolvendo 19 pacientes com edentulismo na região posterior de maxila. De forma aleatória, 24 seios maxilares destes 19 pacientes foram distribuídos entre os grupos teste e controle. O grupo teste abordou 12 seios maxilares que foram enxertados com OBD associado à L-PRF, já o grupo controle abordou outros 12 seios maxilares enxertados apenas com OBD. Após 8 meses de reparo, uma biópsia foi obtida por meio de broca trefina no mesmo... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Platelet and leukocyte-rich fibrin (L-PRF) is an autogenous platelet derivative capable of releasing cytokines and growth factors favorable to tissue repair. For this reason, LPRF has been used in several dental procedures. One of the clinical applicabilities of L-PRF is in maxillary sinus lifting procedures, a technique already established in dentistry that allows the placement of dental implants in the posterior region of atrophic jaws. To assess the effectiveness of using L-PRF, two independent clinical studies were conducted using L-PRF in maxillary sinuses. One of them had the purpose of evaluating the influence of L-PRF in bone regeneration when associated with deproteinized bovine mineral bone (DBB) in maxillary sinus floor elevation procedures, and the other had the purpose of evaluating the effectiveness of L-PRF in the repair of Schneider membranes accidentally perforated during the maxillary sinus lifting procedure. For this, the first study was a randomized controlled clinical study involving 19 patients with edentulism in the posterior region of the maxilla. At random, 24 maxillary sinuses of these 19 patients were distributed between the test and control groups. The test group involved 12 maxillary sinuses that were grafted with DBB associated with L-PRF, whereas the control group addressed 12 other maxillary sinuses grafted only with DBB. After 8 months of repair, a biopsy was obtained using a drill bit on the same axis of insertion of the implants. The biopsie... (Complete abstract click electronic access below)
Mestre

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Calixto, Carlos Alberto [UNIFESP]. "Plasma rico em plaquetas (PRP) por meio de centrífuga de bancada." Universidade Federal de São Paulo (UNIFESP), 2011. http://repositorio.unifesp.br/handle/11600/9936.

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Made available in DSpace on 2015-07-22T20:50:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-05-25
INTRODUÇÃO: O plasma rico em plaquetas (PRP) é a concentração autóloga de plaquetas em pequeno volume de plasma, obtido por centrifugação do sangue total com anticoagulante, sendo considerada importante fonte de fatores de crescimento (FC). A sua utilização enfatiza a regeneração tecidual. OBJETIVO: Obter o plasma rico em plaquetas (PRP) por meio de centrífuga de bancada. MÉTODOS: Foram coletos 40ml de sangue total de oito voluntários, para 72 testes, divididos em três grupos (grupos controle, de uma centrifugação, e de duas centrifugações), com variação da força de centrifugação em 200, 400 e 800g. RESULTADOS: Verificou-se que a concentração, no Grupo Duas Centrifugações, apresentou média inferior ao mensurado no Grupo Controle. O Grupo Uma Centrifugação, com a força centrifuga relativa de 200g, apresentou os maiores valores de contagem (417.750/μl), bem como as maiores concentrações de plaquetas (99,03%), que equivaleu a 1,99 vezes o aumento no número das plaquetas do GCt, enquanto que forças de centrifugação superiores foram insuficientes para o enriquecimento. CONCLUSÃO: Obteve-se o plasma rico em plaquetas (PRP), a partir de centrifuga de bancada, com maior concentração na força de 200g.
BACKGROUND: Platelet-rich plasma is an autologous concentration of autologous platelets in a small volume of plasma, obtained by centrifugation of the whole blood, and have been considered to be a rich source of growth factors. OBJECTIVE: Establish a method to prepare a platelet-rich plasma by centrifugation. METHODS: In this study 40 ml from the whole blood was obtained from eight volunteers, and seventy-two tests were performed in three groups (control, one spin, and two spins), changing the intensity performing from 200, 400 and 800g, to establish the method that achieves the optimal platelet enrichment and concentration. RESULTS: The optimal platelet concentration enrichment obtained with the group of two spins was inferior than values was obtained with the group of one spin. In those groups of one spin the enrichment of 99.03% of the platelet concentration was obtained with the relative centrifuge force of 200g. CONCLUSION: Platelet-rich plasma with high platelets counts can be prepared using the method of centrifugation, with highest concentration with the force of 200g.
TEDE
BV UNIFESP: Teses e dissertações

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Ana, Tadić. "Uticaj preparata koncentrovanih faktora rasta na regeneratorne i reparatorne procese u postekstrakcionim ranama." Phd thesis, Univerzitet u Novom Sadu, Medicinski fakultet u Novom Sadu, 2019. https://www.cris.uns.ac.rs/record.jsf?recordId=110072&source=NDLTD&language=en.

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Ekstrakcija zuba je intervencija prilikom koje se zub vadi iz svog ležišta u zubnoj alveoli. Rana koja nastaje kao posledica ove intervncije je takvog oblika da njene ivice nije moguće približiti jednu drugoj , te zarasta per secundam intentionem. Ekstrakcija zuba ima za posledicu mnogobrojne promene na tvrdim i mekim tkivima alveolarnog nastavka u periodu od nekoliko meseci do godinu dana nakon intervencije. Savremena stomatologija se i dalje intenzivno bavi proučavanjem procesa zarastanja ekstrakcione rane iz potrebe da se što bolje razumeju promene u tkivu koje nastaju po gubitku zuba da bi se mogle prevenirati i/ili usmeriti tako da se omogući kasnija lakša protetska rehabilitacija pacijenata. Mnoge studije su pokazale efikasnost faktora rasta u tokom procesa zaceljivanja tkiva.Opisan je veliki broj tehnika za pripremu autolognih krvnih preparata koji sadrže faktore rasta, ali su njihova praktična primena i efikasnost su dalje nejasni zato što svaka od ovih metoda dovodi do izrade različitog produkta sa različitom biologijom i potencijalnim indikacijama za upotrebu. Ekstrakcije mandibularnog trećeg molara spadaju u jednu od najčešćih intervencija sa kojom se u svom radu svakodnevno sreću oralni i maksilofacijalni hirurzi. Ova hirurška procedura je povezana za postoperativnim efektima koji u velikoj meri utiču na kvalitet života pacijenta kao što su bol, trizmus, edem, infekcija i alveolitis. U literaturi postoje dokazi da aplikacija nekog od autolognog krvnog preparata sa visokim sadržajem faktora rasta u određenoj meri može da poboljša proces zarastanja tkiva i da umanji neželjene propratne pojave hirurške intervencije nakon ekstrakcije mandibularnog trećeg molara. Cilj ovog rada je bio da utvrdimo da li primena autolognih krvnih preparata sa koncentrovanim faktorima rasta ubrzava stvaranje koštanog tkiva u ekstrakcionoj rani, kao i da li utiče na učestalost pojave alveolitisa i pojavu aproksimalinih parodontalnih džepova na susednim zubima nakon hirurške ekstrakcije donjeg trećeg molara. Studija je sprovedena kao prospektivna klinička studija split-mouth dizajna. U studiju je bilo uključeno 30 pacijenata kod kojih je indikovana ekstrakcija oba mandibularna treća molara i kod kojih su ovi zubi bilateralno u približno istom položaju u odnosu na drugi donji molar. Nakon hirurške ekstrakcije u jednu alveolu je aplikovan preparat koncentrovanih faktora rasta. Kontrolnu grupu u istraživanju činilo je 30 zubnih alveola u koje nije aplikovan preparat koncentrovanih faktora rasta. U eksperimentalnu grupu spadalo je 30 alveola u koje su aplikovani preparati koncentrovanih faktora rasta nakon ekstrakcije zuba. Pacijentima je prvi obavezan kontrolni pregled zakazivan za 7 dana nakon intervencije, tokom koga su uklanjane suture, a vršen je i klinički pregled rane i parodontološkom sondom je proveravana dubina parodontalnog džepa na distalnoj površini drugog molara. Druga postoperativna kontrola je zakazivana 4 nedelje nakon intervencije, a treća nakon 8 nedelja i na ovim kontrolama je merena dubina parodontalnog džepa na distalnoj površini drugog molara. Ispitanicima su napravljena tri CBCT snimka operisanih regija i to po sledećoj dinamici- prvi snimak neposredno nakon ekstrakcije zuba, drugi 4 nedelje i treći 8 nedelja po intervenciji. Svaki CBCT snimak je analiziran da bi se prikupili željeni podaci: zapremina šupljine alveole koja je ostala nakon ekstrakcije zuba i gustina novostvorenog koštanog tkiva, što su parametri na osnovu kojih procenjujemo proces zarastanja koštanog tkiva nakon intervencije. Ova metodologija je originalna , obzirom da su do sada korišćene dvodimenzionalne radiografske metode snimanja sa ciljem praćenja koštanog zarastanja nakon ekstrakcije zuba manje precizne i pouzdane. Dobijenu podaci su obrađeni odgovarajućim matematičko-statističkim postupcima. Najznačajniji rezultati istraživanja su zatim tabelarno i grafički prikazani. Na osnovu dobijenih rezultata došli smo do zaključka da iako primena koncentrovanih faktora rasta dovodi do intenziviranja procesa koštanog zarastanja i smanjenja dubine parodontalnog džepa na distalnoj površini susednih zuba, ta razlika nije statistički značajna. Obzirom da ni u jednom slučaju nije došlo do pojave alveolitisa, nismo mogli zaključiti na koji način primena koncentrovanih faktora rasta utiče na učestalost ove komplikacije.
Tooth extraction is an intervention during which a tooth is removed from its socket. A wound that remains after this is of specific size and shape and it heals per secundam intentionem. Where once was a tooth, in following months and years, a large number of changes in composition of hard and soft tissues occure. Haeling of extraction wound in still in focus of contemporary dentistry, since it is imperative to understand all tissue changes in order to prevent and/or gide them and enable prosthodontic rehabilitation of the patient. Many studies confirm a benefitial effect of growth factors douring wound healing. A large number of techniques is developed to prepare autologous blood concentrates containing growth factors, like platelet-rich fibrin (PRF) , but their aplicability and efficancy are still unclear because each of these methods results in product with different biology and physical characteristics, as well as different potential indications. Third mandibular molar extraction is one of the most frequent interventions that oral and maxillofacial surgeon face in their everyday clinical practice. This procedure is usually followed by postoperative effects affecting such as pain, trismus, edema, infection and alveolitis. In contemporary literature there is enough evidence to suport beneficial role of autologous blood preparations in wound healing, and some authors even sugest that they can reduce incidence of postextraction complications afther third molar surgery. The aim of this study was to determin weather concentrated growth factors have beneficial effect on bone healing after tooth extraction, as well as their effect on the incidence of alveolitis and do they reduce pocket depth on distal side of adjacent tooth. This study was conducted as prospective clinical split-mouth designed study. 30 patients with both mandibular third molars indicated for the extraction, in similar position, were included in the study. On the same day surgical removal of both mandibular molars was performed, and in one socket PRF was placed. Patients were scheduled for a check-up and suture removals on the 7th postoperative day. During this visit, as well as after 4 and after 8 weeks, depth of distal pocket of the second molar was measured. CBCT was made on the day of surgery, 4 and 8 weeks afther surgery. On these radiographs volume of the bone defect was measured as well as density of newly formed bone tissue in the socket. This is original methodology, while previous studies used two-dimensional radiography methods in order to evaluate bone healing after tooth extractions, with less precision and liability. We processed and analyzed gained data using appropriate mathematical-statistical methods. According to our data we concluded that application of PRF in the extraction socket improves bone healing and reduces depth of pocket on the adjacent teeth, although this effects are not statistically significant. In our study, alveolitis did not occur neither in control nor in the experimental group, so we could not conclude if the application of PRF has any effect on prevention of this complication.

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Pallotta, Rodney Capp. "Avaliação do efeito da radiação laser de baixa potência e do plasma rico em fibrina na reparação óssea em ratos." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42136/tde-25112014-143302/.

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O reparo ósseo é regulado por fases: inflamatória, proliferativa e remodelação, sendo importante para a qualidade do osso. Terapias têm sido estudadas para ajudar neste processo, como Plasma Rico em Fibrina (PRF) e terapia a laser (LBP). No entanto os estudos são controversos. O objetivo deste estudo foi analisar o impacto da LBP com ou sem PRF no reparo após o defeito ósseo crítico em tíbia de ratos. Os animais foram divididos em grupos: hígido, defeito sem tratamento, defeito tratado com 3 doses de LBP, PRF sozinho e associado com LBP. A fim de analisar o processo de reparo, a tíbia foi removida após 1,3,7,14,21,30 e 60 dias após o procedimento, sem qualquer tipo de tratamento para análise histológica e bioquímica do curso de temporal. Após determinação do tempo ideal, analisamos os diferentes tratamentos. Os resultados mostram que o tempo de curso do procedimento de defeito ósseo induz um aumento de IL - 1b e de fosfatase alcalina, sem alterações no TNF-a e IL-10 e diminuição de IL-6. Tratamento LBP induziu a expressão de IL10 e inibir IL-1 b, TNF-a, IL-6 e fosfatase alcalina. O tratamento associado reduziu a fosfatase alcalina, TNF-a, IL-6 e aumento de IL-10. Conclui-se que o tratamento combinado não apresentara melhora significativa no reparo ósseo.
The bone repair is regulated by phases: inflammatory, proliferative and remodeling and is important for bone quality. Therapies have been studied to help in this process, as Platelet Rich Fibrin (PRF) and laser therapy (LLLT). However the studies are controversial. The aim of this study was to analyze the impact of LLLT with or without PRF repair after the critical bone defects in tibias. The animals were divided into groups: healthy male without blemish treatment, defect treated with 3 doses of LBP, PRF alone and associated with LLLT. In order to analyze the repair process , the tibia was removed after 1,3,7,14,21,30 and 60 days after the procedure, without any kind of treatment for histological and biochemical analysis of the course of time. After determining the optimal time, we analyze the different treatments. The results show that the time course of the procedure of defect induces an increase of IL - 1b and alkaline phosphatase, without changes in TNF- a and IL-10 and reduced IL-6. LBP treatment induced the expression of IL -10 and inhibiting IL-1 b, TNF- a, IL-6 and alkaline phosphatase. The combined treatment decreased the alkaline phosphatase, TNF- a , IL-6 and increased IL-10. We conclude that combined treatment had not produced significant improvement in bone repair.

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FERRACIOLLI, E. "Avaliação das Fibras Colágenas de Feridas Dérmicas de Coelhos Tratadas com Diferentes Fontes de Plasma Rico em Plaquetas." Universidade do Oeste Paulista, 2016. http://bdtd.unoeste.br:8080/jspui/handle/jspui/999.

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The platelet-rich plasma (PRP) is a promising resource in treatment of wounds, considered a non-toxic biomaterial, which when injected in the tissue, increases its regeneration capacity by stimulating growth factors and activating angiogenesis and thus to induce synthesis of collagen. Studies confirm the effectiveness of the autologous gel source of PRP, but the homologous and heterologous gel sources are alternatives when there are no conditions for obtaining the animal's own PRP, however, unknown whether the effect of using these sources in the organization of the collagen fibers during the process scar. Therefore, this study aimed to compare the effects on healing produced after performing dermal wound in rabbits treated with different sources of platelet-rich plasma (PRP) gel (autologous, homologous and heterologous). 36 biopsies of dermal wounds have been used, experimentally induced in 18 adult rabbits, clinically healthy, of New Zealand breed (n = 9 males, n = 9 females), treated and not treated with different sources of PRP. The wounds were sanitized and treated on 0,3,7,10,14 days, and, in 17º day, a biopsy of the wound has been made, for histologic evaluation and observation of the initial phase of remodeling. In order to determine the type of collagen, the biopsy slides passed through polarization technique with dye-red Picrosirius F3BA. Results confirmed that the source of homologous PRP gel proved to be as efficient as the source of autologous PRP gel if compared healing. Other important finding in this study was that heterologous PRP gel use provided a healing process as good as those provided by autologous and homologous gel groups. As tough PRP is rich in growth factors, even these being from other animals of the same species and also of different species, it is noteworthy that even when applied serially, the different types of PRPs have not caused negative effects, as rejection, for example. It has been concluded that the heterologous and homologous sources are safe alternatives and their use contributes positively to an adequate healing process.
O plasma rico em plaquetas (PRP) é um recurso promissor no tratamento de feridas, considerado um biomaterial atóxico, que quando injetado no tecido, aumenta a capacidade de regeneração tecidual por estimular fatores de crescimento e ativar a angiogênese, e assim induzir a síntese de colágeno. Estudos comprovam a eficácia do PRP de fonte autóloga gel, porém as fontes homóloga e heteróloga gel constituem alternativas quando não há condições de obtenção do PRP do próprio animal, porém desconhece-se o efeito do uso destas fontes na organização das fibras colágenas durante o processo cicatricial. Sendo assim, o trabalho teve como objetivo comparar os efeitos na cicatrização após a realização de ferida dérmica em coelhos tratados com diferentes fontes de plasma rico em plaquetas (PRP) gel (autólogo, homólogo e heterólogo). Foram utilizadas 36 biópsias de feridas dérmicas, experimentalmente induzidas em 18 coelhos adultos, clinicamente saudáveis, da raça Nova Zelândia (n= 9 machos, n= 9 fêmeas), tratados e não tratados (controle) com diferentes fontes de PRP. As feridas foram higienizadas e tratadas nos dia 0,3,7,10,14 e no 170 dia foi realizada a biópsia da ferida, para avaliação histológica e observação da fase inicial de remodelação. Para a determinação do tipo de colágeno, as lâminas de biópsia passaram pela técnica de polarização com o corante Picrosirius-red F3BA. Os resultados comprovaram que a fonte de PRP homólogo gel mostrou-se tão eficiente quanto a fonte de PRP autólogo gel em comparação a cicatrização. Outro importante achado no presente estudo foi que o uso PRP heterólogo gel proporcionou um processo de cicatrização tão bom quanto àqueles proporcionados pelos grupos autólogo e homólogo gel. Como o PRP é rico em fatores de crescimento, mesmo estes sendo de outros animais da mesma espécie e também de espécies diferentes, vale ressaltar que, mesmo quando aplicadas de forma seriada, as diferentes fontes de PRP não ocasionaram efeito negativo como, por exemplo, complicações clínicas. Conclui-se que as fontes heteróloga e homóloga são alternativas seguras e que seu uso contribui positivamente para um processo cicatricial adequado.

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Marques, Maria Elisa Marin. "Quantificação de colágeno em feridas dérmicas de coelhos tratados com plasma rico em plaquetas heterólogo gel." Universidade do Oeste Paulista, 2016. http://bdtd.unoeste.br:8080/tede/handle/tede/737.

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Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is useful in the event that obtaining the patient s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gel is capable of promoting proper healing with no adverse reactions, increased collagen content, and faster healing. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation and retraction index of the wounds were measured on days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.
O plasma rico em plaquetas (PRP) tem sido amplamente estudado como um biomaterial para o tratamento de feridas, sendo o produto de fonte heteróloga indicado na impossibilidade de obtenção do sangue do próprio paciente. O presente estudo teve como objetivo, avaliar e comparar a cicatrização em coelhos e quantificar o colágeno de feridas experimentalmente induzidas, denominadas controle e tratadas, com PRP heterólogo gel. A hipótese é que este seja capaz de promover cicatrização adequada sem reações adversas, e promova aumento do colágeno e uma cicatrização mais rápida. Os aspectos clínicos cor, edema, hiperemia, exsudato, crosta, granulação e índice de retração das feridas foram avaliados nos dias 7, 14 e 17 após a lesão. A quantificação do colágeno, através da coloração com Picrosirius e avaliação sob luz polarizada, foi realizada no 17º dia. Houve presença de crosta nos dois grupos em todos os momentos avaliados, com ausência dos demais sinais clínicos. O percentual de contração da lesão e quantidade de colágeno não diferiu entre os grupos. Concluiu-se que o PRP heterólogo gel não foi capaz de aumentar a quantidade de colágeno e acelerar o processo de cicatrização tecidual, no entanto, a cicatrização foi eficiente e semelhante entre os dois grupos e não houve nenhum tipo de reação adversa local. Desta forma, apesar da escassez de estudos encontrados na literatura, o PRP heterólogo gel é uma alternativa de tratamento de feridas, na impossibilidade de uso de outras fontes de PRP.

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Moreira, Valéria Cardoso. "Análise do colágeno e angiogênese de feridas dérmicas de coelhos tratados com PRP autólogo gel." Universidade do Oeste Paulista, 2016. http://bdtd.unoeste.br:8080/tede/handle/tede/733.

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Significant scientific advances have been made in the last ten years to ascertain the effects of the use of platelet-rich plasma (PRP) and its contribution not only to the hemostatic process, but also wound healing, by releasing growth factors that have tissue regenerating induction properties. The effects of autologous PRP gel and the quality of collagen fibers produced were evaluated after induction of dermal wounds in 6 New Zealand rabbits, treated or not with autologous PRP gel. The wounds were followed for 17 days. On days 7, 10 and 17 the animals were evaluated for the morphological and morphometric behavior of the wounds. Regarding the percentage of collagen fibers, there was a greater quantity (P <0.05) in the untreated rabbits (85.7%) compared to those receiving autologous PRP gel (75.0%). However, microscopically, increased expression of collagen fibers was observed in the samples treated with autologous PRP gel, displaying a more homogenous layout and better fiber reorganization, demonstrating a dense network involving all attachments and newly formed vessels characterized by high dermal birefringence. It can be concluded from this study that the use of autologous PRP gel on wounds improved the quality and reorganization of collagen fibers.
Avanços científicos significativos nos últimos dez anos foram feitos para verificar os efeitos do uso do plasma rico em plaquetas (PRP) e a sua contribuição não só para o processo hemostático, mas também para cicatrização de feridas através da liberação de fatores de crescimento que têm propriedades de indução de regeneração tecidual. Avaliou-se o efeito do PRP autólogo gel e a qualidade de fibras colágenas produzidas após realização de ferida dérmica em 6 coelhos da raça Nova Zelândia tratados ou não com PRP autólogo gel. As lesões foram acompanhadas durante 17 dias. Nos dias 7, 10, e 17, os animais foram avaliados quanto ao comportamento das feridas morfológica e morfometricamente. Em relação à porcentagem das fibras de colágeno, houve uma quantidade maior (P<0,05) nos coelhos não tratados (85,7%) comparados aos que receberam PRP autólogo em gel (75,0%). Porém, microscopicamente, observou-se aumento de expressão de fibras de colágeno nos coelhos tratados com PRP autólogo gel, mostrando uma disposição mais homogênea com melhor reorganização dessas fibras, evidenciando uma rede densa que envolveu todos os anexos e com vasos neoformados caracterizados pela alta birrefringência da derme. Pode-se concluir neste estudo que o uso do PRP autólogo gel em feridas melhora a qualidade e a reorganização das fibras colágenas.

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Pedrosa, Ana Sofia Fontes Barbosa. "O Uso de PRF (Platelet Rich Fibrin) na Regeneração Periodontal." Master's thesis, 2017. https://repositorio-aberto.up.pt/handle/10216/107254.

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Pedrosa, Ana Sofia Fontes Barbosa. "O Uso de PRF (Platelet Rich Fibrin) na Regeneração Periodontal." Dissertação, 2017. https://repositorio-aberto.up.pt/handle/10216/107254.

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Chien, Chian-Shiang, and 簡千翔. "Comparison of platelet-enriched fibrin glue and platelet rich plasma gel biodegradable fibrin scaffolds for tissue engineering of cartilage." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/86903733837355446754.

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碩士
臺北醫學大學
醫學科學研究所
97
Articular cartilage has a limited self-regenerative capacity. Tissue engineering using autologous chondrocytes implantation (ACI) provide an promising strategy on cartilage repair, seeding cell on scaffold to culture healthy chondrocytes in vitro, then re-inject into articular cartilage. Platelet-enriched fibrin (PEF) glue are making from autolous platelet rich plasma (PRP). It is a natural materials that has biocompatible and biodegradable properties, can provide 3D environment allow cells do attachment, secretes multiple cytokines for chondrogenesis differentiation and produce extra cellular matrix (ECM). In this study, we observed that PEF glue containing abundant platelets inside the structure, it has good stiffness, mechanical properties obvious greater than PRP gel and bovine fibrin. Besides, PEF glue also has significant higher TGF-β1 and BMP-2 concentration comparing with PRP gel and bovine fibrin. In cell culture, we seeded different cells into PEF glue, PRP gel and bovine fibrin, the MTT assay and mRNA expression of type II collagen, aggrecan indicating PEF glue is the most suitable scaffold in this experimental group. The PAS and Alcian blue staining showed the cells seeded on PEF glue in vivo can produce more proteoglycan and glycosaminoglycan, indicating it has good ability for chondrogenesis differentiation. PEF glue is made by autologous methods, contained multiple growth factors, and do not have the immune problem. It can decrease bacteria infect by injection, making the smallest wound and avoid the secondary surgery. In the future, PEF glue is superior for the material of articular cartilage with tissue engineering.

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Pascoal, Martim de Almeida Nóbrega Correia. "Leucocyte versus advanced-platelet rich fibrin membranes resistance to traction : a comparative study." Master's thesis, 2019. http://hdl.handle.net/10400.14/28469.

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Objetivos: Este estudo teve por objectivo fazer uma comparação directa da resistência à tração entre membranas produzidas com o protocolo L-PRF (Leucocyte-Platelet Rich Fibrin) e A-PRF (Advanced-Platelet Rich Fibrin). Materiais e Métodos: Após a colheita de sangue de uma pessoa saudável e sem histórico de toma de anticoagulantes, procedeu-se à produção de membranas segundo os protocolos de L-PRF e A-PRF, previamente descritos na literatura. De seguida, as membranas (13 para cada protocolo) foram submetidas a um teste de tração, para os quais foram obtidos valores de tração máxima e de tração média. A análise estatística dos dados foi feita com recurso a teste t não pareado. Resultados: Relativamente à tração média o protocolo A-PRF obteve 0.0288 N.mm-2 e o L-PRF 0.0192 N.mm-2 (p<0.05 utilizando teste-t não pareado; n=13). Para a tração máxima registou-se para o protocolo A-PRF o valor de 0.0752 N.mm-2 e L-PRF 0.0425 N.mm-2 (p<0.05 utilizando teste t não pareado; n=13). Conclusão: Com este estudo foi possível concluir que o protocolo A-PRF permite obter membranas com valores de tração máxima e tração média mais elevados do que os obtidos para o protocolo L-PRF, apontando assim para uma maior resistência quando duas forças opostas são aplicadas sobre a membrana. Este facto, associado à otimização das suas propriedades celulares e biológicas, fazem do protocolo A-PRF uma escolha melhor em detrimento do L-PRF.
Purpose: This study aimed at comparing the resistance traction between membranes produced with the protocol L-PRF (Leucocyte-Platelet Rich Fibrin) versus the protocol A-PRF (Advanced-Platelet Rich Fibrin). Materials and Methods: After blood collection of a healthy individual with no history of anticoagulant usage, we produced fibrin membranes according to the protocols L-PRF and A-PRF, previously described in the literature. Afterwards the membranes (13 for each condition) were submitted to a traction test, assessing the maximal traction and the average traction obtained for each membrane. The data was analyzed using unpaired t-test. Results: Regarding average traction, the A-PRF protocol obtained a value of 0.0288 N.mm-2 and L-PRF 0.0192 N.mm-2 (p<0.05 using unpaired t-test; n=13). For maximal traction A-PRF obtained 0.0752 N.mm-2 and L-PRF 0.0425 N.mm-2 (p<0.05 using unpaired t-test; n=13). Conclusion: With this study, we conclude that the A-PRF protocol generates membranes with higher maximal traction average traction scores, which indicates an increased resistance when two opposing forces are applied to it. This fact, associated with the optimization of the cellular and biological properties, make A-PRF a better protocol for the preparation of fibrin membranes.

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Su, Sheng-Fen, and 蘇聖分. "Effects of Matrine and Platelet-Rich Fibrin on Mouse Embryo Development." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/8j53m4.

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碩士
國立屏東科技大學
生物科技系所
105
Martine (Sophocarpidine ) is a nature alkaloid derived from Traditional Chinese Medicine-Sophora root (the root of sophoraflavescens Ait), and it has been documented Compendium of Materia Medica. Matrine has widely used in the viral infection and skin treatment. Recent studies have demonstrated that matrine induces apoptosis in cancer cells and inhibits the melanoma cell proliferation. The potential toxicity of matrine on mouse embryo development however is still unclear. In this study, blastocysts were cultured in the presence or absence of matrine (0, 25, 50 or 100 M ) for 24 hours. Results showed that matrine ( 25, 50 or 100 M ) has inhibition on the total cell number of blastocysts following 24 hour culture. This inhibition of matrine on embryonic cell growth was mediated by a selective effect of matrine on ICM cells without affecting on TE cells. Platelet-rich fibrin (PRF) is a second generation platelet concentrate prepared without anticoagulants. Extracts derived from PRF contain several growth factors, like platelet-derived growth factors (PDGF), insulin-like growth factors (IGF), vascular endothelium growth factor (VEGF) or transforming growth factors (TGF). To learn the potent of PRF whether can protect the embryonic cells from matrine inhibitory effect, we cultured blastocysts in the presence of matrine (100 M) with 10% of fetal bovine serum (FBS) or matrine with 10% of horse PRF extract for 24 hours. Results showed that PRF significantly increased the number of total embryonic cells compared with control group supplemented with FBS. In matrine treated blastocysts, the number of embryonic cells, either in the total or in the ICM cells has significantly improved by PRF.

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Pedro, Beatriz Mancebo Vieira. "The effect of platelet rich fibrin in oral surgery: a literature review." Master's thesis, 2017. http://hdl.handle.net/10451/29500.

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Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2017
To summarize the relevant literature regarding the clinical efficacy of Platelet Rich Fibrin (PRF) in oral surgery and its applications in clinical practice. Introduction: One of the challenges facing clinical research is the development of bioactive surgical additives regulating inflammation and increasing healing. Platelet Rich Fibrin is a second-generation platelet concentrate developed by Dr. Joseph Choukroun, in 2001. The combined properties of fibrin, platelets, leucocytes, growth factors and cytokines makes Platelet Rich Fibrin a healing biomaterial with tremendous potential for bone and soft tissue regeneration (Dohan, D., et al., 2006). Materials and Methods: The research was based on articles published from March 2006 until April 10th 2017 on the databases Cochrane Library and Medline (PubMed), with the combination of the key-words: Platelet Rich Fibrin, Platelet Concentrates, Oral Surgery, Healing and Tissue Regeneration. The included studies were: Meta-analysis, Systematic Reviews and Randomized Control Trials, which were extended afterwards to include Controlled Clinical Trials, Reviews, Cohorts, Case Reports and In vitro studies. Inclusion criteria consisted of articles published in the last 10 years and available in English. Articles were also retrieved after analyzing the reference list of articles previously obtained. Results: The search yielded 1455 articles, of which 86 were selected for inclusion, and 93 additional articles were obtained from the reference lists of other articles, making a total of 179 articles. Discussion and Conclusion: The scientific literature demonstrated safe and promising results related to the use of Platelet Rich Fibrin, defending that it has several advantages and possible indications to be used both in medicine and dentistry. Although many authors advocate positive results, further research with well-designed randomized controlled trials and with long-term follow-up, is necessary to clarify clinical outcomes.

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26

Monteiro, Ana Margarida de Lima Alegre. "Leukocyte and platelet-rich fibrin in the regeneration of periodontal infrabony defects." Master's thesis, 2017. http://hdl.handle.net/10316/81854.

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Trabalho de Projeto do Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina
Introdução: O crescente conhecimento na área da Periodontologia levou ao desenvolvimento de várias técnicas regenerativas, mas também ao reavivar do uso de concentrados plaquetários no tratamento de defeitos periodontais infraósseos. Objetivos: Esta revisão sistemática pretende perceber se a abordagem com fibrina rica em plaquetas e leucócitos (L-PRF) melhora os resultados clínicos e radiológicos, se promove uma melhor qualidade de vida pós-operatória ao paciente e, também, qual o estado da arte relativo à sua aplicação. Metodologia: Foi definida uma questão PICOT, "Em pacientes com defeitos periodontais profundos, qual a eficácia da aplicação de L-PRF, isolado ou em combinação com outros biomateriais, na regeneração de defeitos periodontais, comparativamente à cirurgia de acesso, após um período de cicatrização mínimo de 6 meses?", seguida de uma pesquisa eletrónica nas bases de dados primárias (PubMed e Cochrane) com as palavras-chave: “platelet rich plasma”, “platelet rich fibrin”, “plasma rich growth factors”, “periodontal defect”, “infrabony”, “intrabony”, “bone regeneration” e “periodontal regeneration”; com os conectores boooleanos “AND” e “OR”. Sempre que possível, foram usados termos MeSH. Os critérios de pesquisa incluíam Meta-Análises, Revisões Sistemáticas e Ensaios Clínicos Randomizados, com o resumo disponível, em humanos, publicados nos últimos 10 anos, em inglês ou português. Foi feita uma série de casos clínicos para demonstrar a aplicabilidade do L-PRF na regeneração minimamente invasiva de defeitos infraósseos com bolsas profundas. Resultados: Sete revisões sistemáticas foram incluídas nesta revisão sistemática. Os parâmetros avaliados foram a redução da profundidade de sondagem, o ganho de inserção clínica e o preenchimento ósseo, em mm e em %. A série de casos clínicos, embora sem follow-up aos seis meses, permitiu verificar a ausência de sintomas pós-operatórios (como a dor ou o edema). Discussão: Em relação aos parâmetros clínicos (redução da profundidade de sondagem e ganho de inserção clínica), o PRF parece fornecer em resultados melhores que outros concentrados plaquetários autólogos (APCs). No que respeita aos parâmetros radiológicos (preenchimento ósseo), ainda que com informação limitada, o PRF parece fornecer melhores resultados que outros APCs, como o plasma rico em plaquetas. Os resultados centrados no paciente não estão devidamente analisados nos estudos incluídos, mas a pouca informação existente sugere que o PRF proporciona uma recuperação pós-operatória melhor. Embora tenha algum grau de fiabilidade, a evidência científica existente relacionada com L-PRF ainda necessita de ser melhorada no futuro. Conclusão: Tendo em conta as limitações desta revisão, é possível concluir que a evidência científica existente demonstra uma melhoria dos parâmetros clínicos quando o L-PRF é utilizado. Ainda assim, são necessários mais ensaios clínicos randomizados multi-centro com qualidade e validade científica para provar a dimensão da eficácia desta técnica.
Introduction: The increasing knowledge in the Periodontology field lead to the development of several new regenerative techniques, but also to the revival of the use of platelet concentrates in the regeneration of infrabony defects. Objective: This systematic review aims to understand if the leukocyte and platelet-rich fibrin (L-PRF) approach improve clinical and radiological outcomes of infrabony defects, and if it promotes a better postoperative quality of life of the patient, but also what is the state of art regarding its clinical application. Methodology: A PICOT based question was elaborated “In patients with periodontal infrabony defects, what is the efficacy of L-PRF alone or in combination with other biomaterials in periodontal regeneration, comparatively to open flap debridement, after at least 6 months of healing?”. An electronic literature research was performed in PubMed database and Cochrane Library with the keywords: “platelet rich plasma”, “platelet rich fibrin”, “plasma rich growth factors”, “periodontal defect”, “infrabony”, “intrabony”, “bone regeneration” and “periodontal regeneration”; and the Boolean connectors “AND” and “OR”. The MeSH Terms were applied when possible. As filter criteria, the type of articles included were Meta-Analysis, Systematic Reviews and Randomized Controlled Trial, with abstract text availability, in humans, published in the last 10 years, in English or Portuguese language. A cases series was made to demonstrate the applicability of L-PRF in the minimally invasive regeneration of deep infrabony defects with deep pockets. Results: Seven systematic reviews were included in this systematic review. The analyzed variables were probing pocket depth reduction, clinical attachment level gain and bone fill (in millimeters and in percentage). The cases series, although without follow-up at six months, suggest an improvement in patient’s quality of life, since no postoperative symptoms (such as pain and swelling) were reported. Discussion: Regarding the clinical parameters (pocket depth reduction and clinical attachment level gain), PRF seems to provide better outcomes than other autologous platelet concentrates (APCs). Concerning radiologic parameters (bone fill), although with limited information, PRF still seems to give better outcomes than other APCs, such as platelet-rich plasma. The patient center outcomes are not properly assessed across the studies included, but the scarce information available suggests that PRF provides a better post-operatory recovery Although with a certain degree of reliability, the existing scientific evidence regarding L-PRF still need to be improved in the future. Conclusions: Within the limitations of this study, it can be concluded that the literature demonstrated a statistically significant improvement when L-PRF is applied. Nonetheless, more multicenter randomized clinical trials with quality and scientific validity are needed to prove the dimension of the effectiveness of this technique.

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Jiing-Huei and 趙璟徽. "The effects of platelet rich fibrin on human osteoblasts and the clinical application." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/79834835772888408365.

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碩士
中山醫學大學
口腔醫學研究所
99
Platelet-rich fibrin (PRF) is a second-generation platelet concentrate which allows one to obtain fibrin membranes enriched with platelets and growth factors, after an anticoagulant-free blood harvest. However, limited information is currently available concerning the biologic effects of PRF on osteoblasts. The aim of this study is to provide clear evidence for the clinical use of PRF, we investigated the biologic effects of PRF on human osteoblasts. PRF was obtained by centrifugation at 2700 rpm for 12 minutes with a PC-O2 table centrifuge. At first, light microscope examination of PRF reveals a homogenous fibrin appearance by hematoxylin and eosin stain. Leucocytes were located within the PRF matrix. According to SEM image of PRF, a matrix can be observed of membrane like appearance, arranged by fibrin network. Platelet aggregates are assembled on the matrix surface occasionally. Then primary cultured human U2OS osteoblast cell line were used to evaluate cell viability and proliferation resulting from PRF according to trypan blue and tetrazolium bromide reduction assays. The result shows that PRF did not interfere with cell viability of U2OS cells. PRF was found to increase osteoblast proliferation during 5 day incubation period (p < 0.05). Besides, osteoblasts were observed to attach at the margins of PRF by phase-contrast microscopy. Afterward, exam of U2OS cultures with PRF for 21 days. After Von Kossa staining, mineralisation nodules were easily detectable. This was a significant indication when considering the favorable effect of PRF on bone mineralization. At the end of this study, we present 3 clinical cases with clinical, histologic, and radiographic finding. All of these examinations revealed new bone formation. Taken together, PRF can stimulate osteoblasts proliferation. And PRF was suggested to with a potential role for new bone formation. The application of PRF may provide the benefit for the bone regeneration.

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Pedro, Mara Simões. "Platelet-rich fibrin membranes resistance to traction comparing A-PRF versus A-PRF+." Master's thesis, 2021. http://hdl.handle.net/10400.14/34572.

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Objetivos: O presente estudo teve como objetivo comparar as propriedades mecânicas de resistência à tração e estrutura entre as membranas produzidas por A-PRF (Advanced- Platelet Rich Fibrin) e A-PRF+ (Advanced-Platelet Rich Fibrin+). Materiais e métodos: Recorrendo à colheita de sangue de um dador saudável sem história de uso de anticoagulantes ou imunossupressores, realizou-se a preparação das membranas seguindo as indicações protocolares definidas na literatura para A-PRF e A-PRF+. De um N=16/grupo, 13 membranas de A-PRF e 12 de A-PRF+ foram submetidas ao teste de tração, para a obtenção de valores referentes à tração máxima e tração média. Os dados obtidos foram analisados estatisticamente com o teste t não pareado. Após avaliação desta variável, as membranas foram observadas em MEV (Microscopia Eletrónica de Varredura). Resultados: Foram obtidos em relação à tração máxima, 0.0020 N.mm-2 para o A-PRF e 0.0022 N.mm-2 para A-PRF+. Relativamente à tração média, A-PRF obteve 0.0012 N.mm-2 , enquanto o A-PRF+ obteve 0.0015 N.mm-2 (p˂0,01). Nas observações de superfície com MEV, A-PRF+ mostrou ser o concentrado plaquetário mais poroso, com maior abundância de fibras e preservação celular. Conclusão: Este estudo permitiu concluir que o protocolo A-PRF+ foi capaz de produzir membranas com valores de tração máxima superiores aos obtidos pelo A-PRF, sendo os resultados indicativo de que o protocolo apresentou membranas com maior resistência e capacidade elástica ao serem tracionadas por duas forças opostas. A este fenómeno acrescenta-se a arquitetura demonstrada na matriz de A-PRF+ e as propriedades biológicas otimizadas descritas na literatura.
Purpose: This study aimed to formulate a comparison of the mechanical properties of tensile strength and structural organization between membranes produced by A-PRF (Advanced Platelet-Rich Fibrin) and A-PRF+ (Advanced Platelet-Rich Fibrin+). Materials and Methods: Blood was collected from a healthy donor with no history of anticoagulant or immunosuppressant use, the membranes were prepared following the protocol indications defined in the literature for A-PRF and A-PRF+. From an N=16 for each protocol, 13 membranes of A-PRF and 12 of APRF+ were submitted to the traction test, evaluating maximum and average traction. Data was statistically analyzed using the unpaired t test. Membranes were then carefully observed in SEM (Scanning Electron Microscopy). Results: For maximum traction were obtained 0.0020 for A-PRF and 0.0022 for A-PRF+. Regarding the average traction, A-PRF scored 0.0012 while A-PRF+ obtained 0.0015 (p=0.01 unpaired t-test). Surface morphology observations with SEM, A-PRF+ showed to be the most porous platelet concentrate, with greatest fiber abundance and cell preservation. Conclusion: This study allowed to conclude that A-PRF+ protocol was able to produce membranes with higher maximum traction results than those found for A-PRF, indicating that the protocol with low centrifugation time, presented membranes with better viscoelastic strength when they are stretched by two opposed forces. To this phenomenon is added the architecture demonstrated in the A-PRF+ matrix and the optimized biological properties described in literature. A-PRF+, by the view of the developed findings in this work, a better option compared to A-PRF. Keywords: “Platelet-Rich Fibrin”, “Viscoelastic”, “Tensile Strength”, “Rupture”, “Porosity”, “Low-Speed Centrifugation Concept”.

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Ching, Paula Carmela O., and 秦小玫. "Platelet-Rich Fibrin/Decellularized Cartilage Powderin Articular Cartilage Defect Regenerationin a Rabbit Model." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/fb7t94.

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30

Ko, Pai-Hung, and 柯百鴻. "Development of platelet-rich fibrin (PRF) as biodegradation scaffold for application in cartilage engineering." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/88097638314545469537.

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碩士
臺北醫學大學
醫學科學研究所
96
Because of articular cartilage has a limited ability for self- repair. Thus, treatment of cartilage lesions is a challenge. Osteoarthritis (OA), is known as degenerative joint disease and a type of arthritis that is caused by the breakdown and eventual loss of the cartilage of one or more joints. This thesis investigates to incorporate PRF that involves developmental signals into fibrin for use in cell biological studies and as a regeneration matrix employing tissue-engineering (TE). The goal is to explore the fibrin gel that adds PRF scaffold for in vitro culture and in vivo of chondrocytes proliferation and differentiation. Platelet-rich fibrin (PRF) is a new generation of platelet concentration, it’s abundant in platelet cytokines, like platelet-derived growth factors (PDGF-BB), insulin-like growth factor-1 (IGF-1), transforming growth factor (TGF-??1) and bone morphogenetic proteins (BMP-2). They have the ability of chondrocytes proliferation and differentiation. In the thesis, fibrin scaffolds have two types. One is produced from bovine fibrinogen and thrombin that mix to fabricate the fibrin gel, and the other is add PRF from human blood by centrifugation without anticoagulant into fibrin scaffold. And the fibrins are made two forms, the fibrin gel and it is to make a fibrin sponge by freeze-drying. We set up the primary human chondrocytes and SW-1353 . The primary chondrocytes and SW-1353 are embedded and developed in these scaffolds. Additionally, the agarose scaffold is to be the control. Three types of three-dimension (3D) and two-dimension (2D) culture are fibrin, fibrin with PRF and agarose scaffold are evaluated，which is the adaptive one to enable the chondrocytes proliferation and differentiation . We compare SW-1353 and primary human chondrocytes on 2D and in 3D of three kinds of scaffold. In order to carry out a comparative study, we undertake the inner microstructure of fibrin, PRF sponge that has an interconnected pore structure is observed by scanning electron microscope (SEM). And we observe cell morphology by using microscopy. To quantify the concentration of growth factors in the PRF exduates, PDGF-BB, IGF-1, TGF-??1, and BMP-2 are stimulators that correlated with proliferation and differentiation by ELISA. MTT assay and RT-PCR are used for estimate cell survivability and the mRNA expression of type II collagen and aggrecan. To detect the glycosaminoglycan (GAG) from chondrocytes are in different scaffold by PAS and Alician blue staining. The results showed that the chondrocytes on 2D and in 3D fibrin + PRF scaffold structure could provided the more available proliferation and differentiation than cells just on 2D and in 3D fibrin or agarose. It is concluded that the fibrin with PRF gel including abundant cytokines and growth factors is a promising three-dimension scaffold of cells for cartilage tissue engineering.

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Hsieh, Song-Huan, and 謝松桓. "The Effect of Platelet Rich Fibrin on Velvet Cell Proliferation in Formosan Sika Deer." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/5vzwu6.

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碩士
國立屏東科技大學
生物科技系所
106
Recent studies have shown that stem cells can be isolated from various tissues such as bone marrow, adipose tissue. Stem cell therapy is a most efficient means of medical; it can be used as a mammalian organ repair and cell renewing. Embryonic stem cell therapy is ethical controversy, but adult stem cells therapy is noncontroversial. However, the primary problem is that not all tissues have encountered isolated stem cells, and the percentage of stem cells isolated is very low. Deer velvet regeneration in mammalian organs is rare. The key to understand that stem cells is able to accomplish such a rapid tissue formation (up to 1cm/day ) platelet-rich fibrin (PRF), which is a new generation of platelet concentrates derived by platelet rich plasma (PRP). Many studies have reported that PRF contain many cell growth factors, such as platelet derivative growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-I (IGF-I) etc., these growth factors are able to promote cell migration, proliferation, differentiation, angiogenesis or collagen synthesis. However, in vitro culture of deer velvet cell, the growth rate is far behind then the deer velvet cell grow on the deers body. Our object was investigated effect of deer velvet cell culture support rabbit PRF in vitro. Using flow cytometer analysis to determine the velvet cell surface marker, we found that velvet cells expression stem cell specific marker of CD271 (3.7-4.4%). Using CFSE (carboxyfluorescein succinimidyl ester) to analysis antler cells in different cell-line passage growth performance, we can find that cells doubling time is about 3 days (culture condition DMEM+10%FBS, 5%CO2, 37℃) and the more number of cell subculture, the time cell doubling gradually increased. To explore the possibility of stem cell populations within deer antlers, we cultured deer velvet cells in 96 well plate, and using MTT assay to estimate growth performance, we find that velvet cells in low-density (3.03x103 cells/cm2) cellular exerted a better rare compared with high-density (3.03x104 cells/cm2) culture, velvet cells doubling time were about 3 day and 6 day. the data show that deer velvet cell had a better proliferation if cultured on lower density. We using MTT assay to estimate growth performance, we find that velvet cell culture supported 2.5-5% PRF have a best growth performance compare with control group (PRF = 0%) or higher group (PRF = 10%). Based on the above, we can know that Formosan sika deer velvet contains stem cells, and it may have potential as a mammalian organ repair and renew, and for the deer velvet culture in vitro, the best condition is lower cell density (3.03x103 cells/cm2) and support a small amount PRF(2.5-5%).

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32

LIU, KENG-CHANG, and 劉耿彰. "Effects of Platelet-Rich Fibrin (PRF) on Regeneration of Muscle, Bone, and Intervertebral Disc." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/p8z6hd.

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博士
東海大學
畜產與生物科技學系
107
Platelet concentrates including platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are becoming innovative tools of regenerative medicine. Autologous PRP is derived from an individual's whole blood then centrifuged to remove red blood cells and leucocytes. The remaining plasma has a 5- to 10-fold higher concentration of growth factors than whole blood. These growth factors have been found to promote natural healing responses, and hence PRP is widely used in orthopedic and sports medicine to relieve pain in musculoskeletal injuries. Platelet-rich fibrin (PRF) is a newer generation of platelet concentrates and has being used as an adjunctive autologous biomaterial to promote bone and soft tissue healing and regeneration. This thesis included an in vitro study and 3 clinical trials, and the objectives were to investigate the roles of PRF on regeneration of muscle, tendon, bone, and intervertebral disc (IVD). In vitro study, we investigated the biologic effects of pig PRF on the proliferation of different cells, including rat L8 myoblasts, mouse RAW 264.7 monocytic cells which are precursors of osteoclasts, mouse L929 fibroblasts, and porcine endothelial cells (PEC). L8 cells and L929 cells cultured with PRF showed a decrease in cell number when compared to the control group in which horse serum was used as a control. RAW 264.7 cells and PEC cultured with PRF, on the other hand, increased cell number. These results suggested that PRF had negative effect on the proliferation of L8 and L929 cells, however, expressed positive regulation on RAW 264.7 cells and PEC. In clinical trials, we investigated the clinical effects of PRF on bone fusion in spinal fusion surgery and IVD regeneration after endoscopic discectomy. Posterior lumbar decompression and 2 transforaminal interbody fusion (TLIF) is a safe and effective surgery for degenerative spinal diseases. Several options of bone graft are available but autologous iliac crest bone grafting (ICBG) is still the gold standard. However, some morbidity is associated with ICBG harvesting such as infection, fracture, or donor site pain. To avoid donor site morbidity, various allograft materials have been used as a substitute but are still questionable as to the effectiveness and safety. In our study, allograft combined with autogenous bone marrow and with/without PRF was used to compare with autologous ICBG in TLIF surgery. After at least 12 months follow-up, the fusion rate in single level TLIF was 100% in both autograft group and allograft with PRF group, while the fusion rate of allograft without PRF was 77.8%. The results demonstrated positive effect of PRF on bone fusion in TLIF. Discogenic low back pain (DLBP) is associated with degeneration of IVD and usually is a complex problem regrading to diagnosis and treatment. It is suspected that the pain source of DLBP comes from the torn lesion of annulus fibrosus (AF) which is located on the outer surface of IVD. We applied percutaneous endoscope to treat torn AF in patients with DLBP and the satisfactory outcomes were more than 85% in selected patients at two years follow-up. The procedure is effective and minimally invasive because only inflammatory tissues on AF and loose nucleus debris are removed under clear endoscopic vision. To investigate the effect of PRF on regeneration of IVD, we inserted PRF into IVD in 6 patients who were diagnosed with DLBP and had undergone endoscopic treatment. Autogenous PRF was inserted into nucleus at the end of endoscopic procedure. At 12 months follow-up, 5 of 6 patients showed excellent results and MRI T2-weighted signal of involved IVD did not show further degenerative change. This adjunctive biomaterial expressed beneficial effects on clinical outcomes and radiologic results.

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33

Said, Sherif Yousri. "The effect of platelet rich fibrin (PRF) on inter-proximal papillary height around dental implants." Thesis, 2016. https://hdl.handle.net/2144/26402.

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The original study design called for twenty patients. Fourteen patients with 13 implants have thus far been recruited for a prospective proof of principle study to assess the effect of addition of Platelet Rich Fibrin (PRF) on interproximal papillary height (I.P.H.) at the restorative line angles when used during the second stage uncovering procedure for dental implants. The secondary objective of this study was to assess the effect on the height of the direct interproximal tissue thickness (I.T.T.) at 6 weeks following uncovering. Implants placed 3 or more months prior at Boston University School of Dental Medicine Periodontics Department were scheduled to be uncovered by the principal investigator. Second stage uncovering was performed with a conventional mid-crestal incision to place standard healing abutments. PRF membrane(s) were inserted around the implants before flap closure in an attempt to augment or thicken the interproximal papillary area. The I.P.H. was measured at the four line angles of each abutment at the highest aspect of the interproximal tissue immediately following the uncovering procedure and at 2, 4 & 6 week intervals. I.T.T. was also measured prior to surgical exposure and at 6 weeks following the uncovering procedure. Results: The use of PRF membranes around dental implants did not significantly improve I.P.H. However, a positive correlation was observed between the use of P.R.F and I.T.T. Further studies are warranted to assess the effect of PRF on the peri-implant papillary tissue.

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34

Chih-Lung and 吳志隆. "Platelet-rich fibrin increases cell attachment, proliferation, and collagen-related proteins expression of human osteoblasts." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/94277273421683896394.

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博士
中山醫學大學
醫學研究所
100
Background: Platelet-rich fibrin (PRF) by Choukroun’s technique is derived from anautogenous preparation of concentrated platelets without any manipulation. PRF was found to increase osteoblast growth and proliferation. However, the underlying mechanisms are not yet completely understood. This study was to determine the effects of PRF on cell attachment, proliferation, phosphorylated Akt, heat shock protein 47 (HSP47), and lysyl oxidase (LOX) expression on human osteoblasts. Methods: Blood collection was carried out from ten healthy volunteers. Cell attachment and proliferation were measured by colorimetric assay with WST-1 and alamlar blue in human osteoblast cell line U2OS cells, respectively. Western blot was employed to evaluate the expression of p-Akt, HSP47, and LOX. Results: PRF alone was found to stimulate U2OS cell attachment as compared with untreated controls (p<0.05). PRF was found to increase osteoblast proliferation during 5 day incubation period (p<0.05). PRF was found to increase Akt phosphorylation in a time-dependent manner (p<0.05). Collagen-related proteins HSP47 and LOX were significantly elevated by the stimulation with PRF as compared with untreated controls (p<0.05). Conclusions: It is suggested that PRF is capable of increasing osteoblast attachment, proliferation and simultaneously upregulating collagen- related proteins production. These actions in combination would effectively promote bone regeneration.

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35

Ferreira, Helena Sofia Andrade Estrada Soares. "L-PRF no Tratamento de Recessões Gengivais." Master's thesis, 2018. http://hdl.handle.net/10316/81919.

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Trabalho Final do Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina
Introdução: No recobrimento radicular de recessões gengivais (RG) têm sido procuradas alternativas ao enxerto de tecido conjuntivo (ETC), de modo a evitar a morbilidade induzida na sua recolha. As membranas de fibrina rica em leucócitos e plaquetas (L-PRF) têm sido testadas pela sua facilidade de obtenção e aporte em fatores de crescimento.Objetivo: Este trabalho pretende fazer uma revisão sistematizada sobre a utilização do L-PRF em associação a técnicas cirúrgicas de recobrimento radicular, bem como apresentar um caso clínico exemplificativo do modo de utilização deste material.Materiais e Métodos: Pesquisa efetuada nas bases de dados electrónicos primária PubMed/MEDLINE e secundária Embase, limitada a Meta-análises e Revisões Sistemáticas de estudos clínicos randomizados em humanos, publicadas nos últimos 10 anos, tendo sido selecionados artigos entre Janeiro de 2008 e Janeiro de 2018, em língua portuguesa e inglesa. Complementou-se esta pesquisa electrónica com uma pesquisa manual nas revistas Journal of Clinical Periodontology e Journal of Periodontology.Resultados: Selecionaram-se 4 revisões sistemáticas. O retalho de reposicionamento coronário (CAF) associado a um número variável de membranas de L-PRF foi a técnica mais estudada, apresentando resultados bastante satisfatórios, mas pouco consensuais, em termos de percentagem de recobrimento radicular e de benefícios adicionais no conforto pós-operatório e cicatrização. Conclusão: A utilização das membranas ricas em leucócitos e plaquetas (L-PRF) no tratamento de recessões gengivais (RG) necessita de investigação adicional, com vista à determinação do protocolo cirúrgico mais previsível e à comprovação dos benefícios clínicos e biológicos deste material.
Introduction: For the root coverage of gingival recessions , alternatives to connective tissue graft (CTE) have been sought in order to avoid the morbidity induced by its collection. Fibrin membranes rich in leukocytes and platelets (L-PRF) have been tested for their ease of production and contribution to growth factors.Aims: This work intends to perform a systematic review on the use of L-PRF in association with surgical techniques for root coverage, as well as to present a clinical case illustrative of the use of this material.Materials and Methods: Research was conducted in the primary (PubMed / MEDLINE) and secondary (Embase) electronic databases, limited to Meta-analyzes and Systematic Reviews of randomized clinical trials in humans, published in the last 10 years, selecting articles between January 2008 and January 2018, in Portuguese and English. This electronic research was supplemented with a manual search in the Journal of Clinical Periodontology and Journal of Periodontology.Results: Four systematic reviews were selected. The coronary repositioning flap (CAF) associated with a variable number of L-PRF membranes was the most studied technique, presenting quite satisfactory, but not very consensual, results in terms of percentage of root coverage and additional benefits in postoperative comfort and healing.Conclusion: The use of leukocyte and platelet-rich membranes (L-PRF) in the treatment of gingival recessions requires further investigation, in order to determine the most predictable surgical protocol and to prove the clinical and biological benefits of this material.

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Loureiro, Ana Teresa Gaspar Nunes. "Regeneração periodontal de defeitos de furca com Fibrina Rica em Plaquetas E Leucócitos (L-PRF)." Master's thesis, 2018. http://hdl.handle.net/10316/82191.

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Trabalho Final do Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina
Introdução: Os defeitos de furca representam um desafio na área regenerativa periodontal, tendo sido propostas diversas abordagens na expetativa de diminuir a imprevisibilidade do seu tratamento. Com o desenvolvimento de protocolos otimizados e simplificados, os concentrados plaquetários autólogos (CP) têm ganho maior visibilidade, com uma potencial aplicação na regeneração destas lesões. Objetivos: Este trabalho tem como objetivo avaliar a eficácia e aplicabilidade dos concentrados plaquetários, em particular, da fibrina rica em plaquetas e leucócitos (L-PRF), na regeneração de defeitos de furca, através de uma revisão do tipo sistematizada e apresentação de um relato de caso clínico. Metodologia: Foi estabelecida uma questão PICOT: “Em pacientes com molares com defeitos de furca grau II, qual a eficácia da aplicação isolada de concentrados plaquetários, ou combinada com outros materiais, quando comparada com outros tratamentos conservadores ou regenerativos, após um follow-up mínimo de 6 meses?”. A pesquisa eletrónica foi realizada nas bases de dados PubMed, Embase e Cochrane com palavras-chave previamente definidas e seletivamente conjugadas com os conectores booleanos “AND” e “OR”. Apenas revisões sistemáticas ou ensaios clínicos randomizados, em humanos, foram incluídos, sem restrições temporais. O caso clínico foi realizado mediante técnicas cirúrgicas minimamente invasivas utilizando o L-PRF como monoterapia. Resultados: Foram incluídas 4 revisões sistemáticas e 9 ensaios clínicos randomizados. Os resultados dos CP revelaram-se superiores ao desbridamento cirúrgico simples, com ganhos significativos de CAL. O L-PRF demonstrou resultados superiores, de forma mais consistente, quando comparado com os demais CP. Relativamente ao caso clínico, verificou-se a ausência de complicações pós-operatórias e um ganho significativo de inserção clínica e uma reversão positiva no encerramento da furca com melhoria do prognóstico dentário. Discussão: Relativamente à informação disponível na literatura, os resultados obtidos com os CP, em geral, e com L-PRF, em particular, são sobreponíveis às demais técnicas regenerativas. A fibrina rica em plaquetas e leucócitos parece mostrar resultados superiores e mais previsíveis que o plasma rico em plaquetas. A escassez de estudos que comparam a fibrina rica em plaquetas e leucócitos com outras técnicas convencionalmente utilizadas, apontam para uma proximidade nos resultados obtidos. O encerramento dos defeitos continua a ser o parâmetro mais imprevisível, sobre o qual não existe informação suficiente na literatura. Os parâmetros centrados no paciente foram sumariamente abordados. Conclusão: O L-PRF demonstrou ser eficaz na melhoria dos parâmetros avaliados, tanto em monoterapia como em combinação com outros materiais, comparativamente ao desbridamento cirúrgico e aos demais concentrados plaquetários. Além disso, o uso do L-PRF posiciona-se como uma alternativa às abordagens convencionais sendo uma opção com justificação biológica, clínica e económica, merecendo futura exploração. Há necessidade de realizar mais estudos clínicos controlados e aleatorizados, com amostras representativas, que permitam comprovar a sua validade científica, bem como impulsionar o total potencial desta modalidade na regeneração periodontal de defeitos de furca.
Introduction: Furcation defects represent a challenge in the periodontal regenerative area, and several approaches have been proposed in order to reduce the unpredictability of its treatment. With the development of optimized and simplified protocols, autologous platelet concentrates (CP) have gained greater visibility, with a potential application in the regeneration of these lesions. Objectives: The objective of this study is to evaluate the efficacy and applicability of platelet concentrates, in particular, leucocyte and platelet-rich fibrin (L-PRF), in the regeneration of furcation defects, through a systematic review and presentation of a clinical case report. Methodology: A PICOT question was established: "In patients with molars with grade II furcation defects, what is the efficacy of the isolated application of platelet concentrates, or combined with other materials, when compared to other conservative or regenerative treatments, with a minimum of 6-months' follow-up? ". The electronic search was performed in the PubMed, Embase and Cochrane databases with previously defined and selectively conjugated keywords with the "AND" and "OR" Boolean connectors. Only systematic reviews or randomized controlled trials in humans were included, with no time constraints. The clinical case was performed using minimally invasive surgical techniques using L-PRF as monotherapy. Results: Four systematic reviews and nine randomized clinical trials were included. The CP results were superior to simple surgical debridement, with significant CAL gains. The L-PRF demonstrated superior results, more consistently, when compared to the other CPs. Regarding the clinical case, it was verified the absence of postoperative complications and a significant gain of clinical insertion and a positive reversal in the closure of the furca with improvement of the dental prognosis. Discussion: Regarding the information available in the literature, the results obtained with CP in general and with L-PRF, in particular, are superposable to the other regenerative techniques. Leucocyte and platelet-rich fibrin appears to show higher and more predictable results than platelet rich plasma. The scarcity of studies comparing leucocyte and platelet-rich fibrin with other conventionally used techniques point to a proximity in the results obtained. The closure of defects remains the most unpredictable parameter, for which there is insufficient information in the literature. Patient-centered parameters were summarily addressed. Conclusion: L-PRF has been shown to be effective in improving the parameters evaluated, both in monotherapy and in combination with other materials, compared to surgical debridement and other platelet concentrates. In addition, the use of L-PRF stands as an alternative to conventional approaches, being an option with biological, clinical and economic justification, deserving future exploration. There is a need to carry out more controlled and randomized clinical studies with representative samples, to confirm their scientific validity, as well as to boost the full potential of this modality in the periodontal regeneration of furcation defects.

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Lin, Sheng-Chuan, and 林昇全. "Application of Autologous Mesenchymal Stem Cells, Coenzyme Q10 and Platelet-rich Fibrin for Acute Myocardial Infarct in Porcine." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/66771978083689275034.

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博士
國立屏東科技大學
熱帶農業暨國際合作系所
100
Abstract Student ID: P9822001 Title of Thesis: Application of Autologous Mesenchymal Stem Cells、 Coenzyme Q10 and Platelet-rich Fibrin for Acute Myocardial Infarct in Porcine Total Page: 104 Name of Institute: Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology. Graduate Date: July, 2012 Degree Conferred: Ph.D. Name of Student: Sheng-Chuan Lin Advisor: Professor Yan-Der Hsuuw Professor Tzong-Fu Kuo The Contents of Abstract in This Thesis: Stem cells therapy is the “process of replacing or regenerating cells, tissues or organs to restore or establish normal function”. Stem cells therapy also has the potential to solve the problem of the shortage of organs. Medicine refers to a group of biomedical approaches to clinical therapies that may involve the use of stem cells, including the injection of stem cells or progenitor cells (cell therapies). Stem cell research application has gotten more and more important achievements now. Especially with regard to the methods to cultivate stem cells or to obtain progenitor cells including myocardiocyte, nerve cells, chondrocytes, hepatocytes, renal cells, lung epithelial cells, etc. In this study, we isolated, cultivated and applied the mesenchymal stem cells in middle animals (pig, cat and dog) regenerative medicine. Wish to create the regenerative medicine therapy models of middle animals. CoQ10 is widely distributed in animals and humans. It plays an important role in cellular energy production in the mitochondrial respiratory chain. CoQ10 has been demonstrated to have a potential role in the prevention and treatment of heart diseases by improving cellular bioenergetics. Cardiogenic shock is a state of inadequate tissue perfusion induced by cardiac disorders. The incidence of cardiogenic shock complicating acute myocardial infarction ranges from 5% to 10% in human being. The most frequent cause of acute MI is associated with myocardial plague (occurs over a period of years or decades). Within 30 minutes of an occlusion on the first branch of left coronary artery, irreversible myocardial cells damage was occurred. The main change is death (necrosis or apoptosis) of myocardial infarct tissue. Cell therapy perhaps presents new opportunities to enhance cardiac performance through trans- differentiation of the injected stem cells into cardiomyocytes to replace or repair damaged tissues or cells. Experimental data have demonstrated that cell therapy has the potential to repair the damaged myocardium. This expectation and the clinical need for an effective treatment to reduce the injury sustained during an acute MI and to repair the failing heart have accelerated the performance of clinical trials mainly using mesenchymal stem cells (MSC). Platelet-rich fibrin contains several growth factors mainly include platelet derivative growth factor (PDGF–A,B), transforming growth factor(TGF-β), vascular endothelial growth factor (VEGF) and epidermal growth factor(EGF). Platelet growth factors exhibit chemotactic and mitogenic properties that promote and modulate cellular functions involved in tissue healing, cell regeneration and cell proliferation. Platelet-rich fibrin extracts implantation for acute myocardial infarct show optimally in infarct area decrease and new angiogenesis. Fibrosis formation was unclear in myocardium infarct area. Heart function measurement demonstrated improvement of left ventricular ejection fraction (LVEF) and fraction shortening (FS) in platelet-rich fibrin extract groups. The progression of renal disease is characterized by the development of glomerulosclerosis and interstitial fibrosis. Patient with end-stage renal disease have an increased risk of cardiovascular disease. The reduction of nephron mass leads to hypertrophy, hyperfiltration, systemic hypertension and glomerulosclerosis. The advancement of stem cell biology has helped in designing stem cells as a therapeutic tool to accelerate tissue repair. Stem cell therapy is becoming a more viable therapy for chronic kidney injury. Immune modulation and anti-apoptotic effects have been associated with this improvement, suggesting that MSCs can act through glomerulosclerosis mechanisms. We used intra-renal artery autologous MSCs injection to halt fibrosis in a clinic case of feline CKD. We paid attention to clinic renal function recovery after MSCs transplantation. We demonstrated that these cells could halt the process of fibrosis with an improvement in renal function. Mesenchymal stem cells are a leading candidate for cell-based therapies. We introduce artificial acute myocardial infarct model and spontaneous chronic kidney disease cells therapy .In conclusion, a combination therapy of mesenchymal stem cells transplantation plus CoQ10 supplementation or platelet-rich fibrin extract for acute myocardial infarct showed promising results in vivo. Left ventricular function was notably improved with only small areas of fibrosis formation. Clinical results showed the optimal direction that the patient demonstrated blood urea nitrogen and creatinine improvement after intra-renal artery mesenchymal stem cells transfer. Keywords: Acute myocardial infarction, Chronic glomerulonephritis, Chronic kidney diseases, Ischemic and reperfusion injures, Mesenchymal stem cells, Stem cell therapy, Uremia

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Peer, Fatima. "Comparing clinical outcomes of connective tissue grafts to platelet rich fibrin in gingival recession treatment: an extended case series." Thesis, 2018. https://hdl.handle.net/10539/25233.

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A Research Report submitted to the School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa, in partial fulfilment of the requirements for the degree of Master of Science in Dentistry, February 2018.
Aim This study set out to evaluate the clinical and aesthetic outcomes of connective tissue grafts (CTG) to platelet rich fibrin (PRF) in treating gingival recession. It was hypothesised that PRF could be as effective as CTGs in treating recession with improved aesthetic results. To the best of my knowledge this clinical study is unique in the South African setting in that an objective aesthetic scoring system was used to report on aesthetic changes and also this study was patient based to determine patient satisfaction with aesthetic outcomes. Methods and materials This six month study was an extended case series with a randomised split-mouth design. Six patients with a total of twenty two sites underwent treatment. However, only five patients fulfiled the study’s follow-up requirements. The patient who failed to comply with the follow-up appointments was disqualified from the study. Each site was paired with a similar lesion on the opposite or contralateral side and randomly assigned to the CTG (control) or PRF (test) treatment. Six variables were recorded over the study period. These variables were probing depth, recession depth, recession width, clinical attachment level, keratinised tissue width and gingival thickness. These were measured at the following intervals: 0, 8, 12, 16 and 24 weeks. Photographs were taken at baseline and at 24 weeks to evaluate aesthetics using the Pink Esthetic Score. At the end of the study period, patients were given a questionnaire to assess their satisfaction with treatment outcomes. Results and Conclusions Both treatments improved the clinical outcomes but CTGs demonstrated improvements at a greater number of sites than PRF (60% to 30% respectively). The aesthetic scores improved at four sites for both CTGs and PRF with only one site in each group scoring lower at the end of the study. The aesthetic scores at the remaining sites did not change over the study period. Therefore, both CTGs and PRF demonstrated the potential to improve or maintain aesthetic results. Analyses of the patient questionnaire showed that patients were satisfied with the aesthetic outcomes of both treatments. The results from this study indicate that both CTGs and PRF membranes can be effective in treating gingival recession and both treatments can improve clinical and aesthetic outcomes.
XL2018

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Tzu-ChingYu and 余紫菁. "Polycaprolactone Combined with Platelet-Rich Fibrin and Adipose-Derived Stem Cells for Endothelialized Small Caliber Vascular Graft in Bioreactor System." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/22198699607515836921.

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碩士
國立成功大學
生物醫學工程學系
104
According to the report published from World Health Organization (WHO), cardiovascular diseases (CVDs) rank number one the cause of death globally. Moreover, there were estimated 17.5 million people died from CVDs in 2012. The patients suffering serious vascular disease have to receive the vascular graft surgery. There are two common vessel substitute resources, one is autologous vessels, and the other is artificial vascular graft. Comparing to autologous vessel, the artificial vessel has some advantages, such as low-cost and easy to manufacture. However, the patency of small caliber vascular graft implantation surgery is only near 50 % within 3 years. It is because of lacking the endothelium layer, that the artificial graft cannot regulate the vascular physiology conditions. Further, it leads to thrombosis, even intimal hyperplasia. In the recent researches of vascular tissue engineering, fabricating an endothelial layer vascular graft is a major issue. In this study, adipose-derived stem cells (ADSCs) were seeded on the biodegradable PCL which was modified with platelet-rich fibrin (PRF) scaffold. And this scaffold was cultured in the dynamic conditions to form an endothelium vascular graft. In the part I of the study, 2 mm of the inner diameter, 4 mm of the outer diameter, and 5 cm of the length PCL scaffold was fabricated by salt-leaching method. The porosity of the scaffold was about 75 %, on the other hand, the pore size was around 150~250um. Then, the scaffold was immersed in the mixed solution of ADSCs suspension and platelet-rich plasma (PRP) overnight. The scaffold would be cultured in the static for 3 weeks. In the result, it showed that adding PRF can increase the cell attachment on the scaffold, which was modified the hydrophobic problem of polycaprolactone (PCL). In addition, the growth factors in the PRF not only enhanced ADSCs proliferation, but also induced the endothelial cells differentiation from ADSCs. In the part II of the study, the ECs containing PRF/PCL scaffold was cultured in the dynamic condition for a week. The dynamic condition was made of the bioreactor system with two different fluid waveform, laminar flow and pulsatile flow. To find out the better culture environment for the vascular scaffold, there were two kinds of designed groups the static culture, and the dynamic culture, which included the laminar flow condition and the pulsatile flow condition. In the result, comparing to the static condition, the scaffold cultured in the dynamic condition showed more type I collagen and higher Young’s modulus. On the other hand, the ECM formation and the burst pressure were increased in the groups of the pulsatile condition. Moreover, the ECs aligned at the luminal side of scaffold after receiving the pulsatile stimulation. In summary, the combination of PRF, PCL vascular scaffold, and ADSCs were successful to form the endothelial layer in the inner surface and the ECM within the scaffold. Finally, the vascular scaffold became structural and functional better through the mechanical stimulation from bioreactor system.

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Liao, Chen-Hao, and 廖政豪. "The Effect of Platelet-rich Fibrin (PRF) Releasate Combined with Bone Marrow Stem Cells on Osteoporosis with a Mouse Model." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/39221159761768569916.

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碩士
臺灣大學
獸醫學研究所
103
Stem cells are a kind of undifferentiated cells which may differentiate into other cell types. And they can also divide indefinitely into stem cell line, but may become cancer cells at about the tenth generation. We collect the bone marrow stem cells (BMSCs) and differentiate BMSCs into osteoblast in vitro after cell sorting by flow cytometer. Platelet-rich fibrin (PRF) is a natural source of growth factors, which contains a large amount of growth factors that can facilitate bone regeneration, Such as: TGFb-1 (Transforming growth factor) , PDGF (platelet-derived growth factor) , VEGF (vascular endothelial growth factor) and Matrix proteins (thrombospondin-1, fibronectin, vitronectin). The growth factors secreted from PRF will cause cell transformation by promoting cell proliferation、matrix formation、bone formation and synthesis of collagen. We co-cultured mouse bone marrow stem cells with platelet-rich fibrin releasate (PRFr), to investigate whether the release would improve stem cell proliferation and promote cell differentiation or not. The results show that proliferation rate of the bone marrow stem cells which co-cultured with releasate were more rapid, and cell viability was much higher. Therefore, we deduced that platelet-rich fiber release solution have significant assistance on the growth and differentiation of bone marrow stem cells. To evaluate therapeutic efficacy of cell-based therapy in osteoporosis: a bone marrow stem cells (BMSCs) and platelet-rich fibrin releasate (PRFr) were used for osteoporosis treatment. An osteoporosis was established with a mouse model by ovariectomy (OVX). Transplantation of BMSCs, PRFr and BMSCs + PRFr into OVX mice for investigating the therapeutic potential for bone regeneration and recovered bone mass loss. OVX or sham operations were performed on virgin ICR mice at 16-weeks old, which were randomLy divided into three parts: Non-surgical, SHAM, and OVX. 6 mice in Non-surgical group was no surgery, 6 mice in SHAM group was subjected to sham surgery, and 30 mice in OVX group mice will accept four different treatments (1)Control group,non injection (2)BMSC-I group, injected BMSCs 3x105 cells/0.6mL PBS once a week for one weeks ; (3)PRF-I group, injected PRFr 0.6mL once a week for one weeks ; (4)BMSCs + PRF-I group, injected BMSCs 3x105 cells combine 0.6 mL PRFr) once a week for one weeks. (5)BMSC-IV group, injected BMSCs 1.2x106 BMSCs/0.6mL PBS once a week for four weeks; (6)PRF-IV group, injected 2.4mL PRFr once a week for four weeks; (7)BMSCs + PRF-IV group, injected 1.2x106 BMSCs / 2.4mL PRFr once a week for four weeks; (8)BMSCs + PRF-IV-i group, intra bone injected 1.2x106 BMSCs/ 2.4 mL PRFr once a week for four weeks. At 8 weeks after implantation, bone mass and its turnover were analyzed by micro CT, We use scanned the left tibia portion of the growth plate at the proximal 0.5-1.5mm, and analyzed bone mineral density, the percentage of bone tissue, trabecular number, trabecular thickness and trabecular separation. Then sacrifice to analyze their efficacy by histomorphometry. A statistically significant difference between the experimental and control groups was observed. BMSCs + PRFr transplants were shown effective in restoring bone mineral density. These findings indicated that the mixture of BMSC and PRF releasate could potentially be an effective agent in the treatment for osteoporosis

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Tseng, Chia-Chuan, and 曾嘉川. "A novel method for rabbit stifle joint cartilage regeneration with injectable platelet-rich fibrin releasate and bone marrow stem cell." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/63171296554476847001.

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碩士
國立臺灣大學
獸醫學研究所
102
Abstracts Osteoarthritis, is a degenerative joint disease accompanied with non-inflammatory articular cartilage lesion, losing of extracellular matrix and develop into cracks, erosion, subchondral sclerosis, osteophytes and loss of joint function eventually. Unlike other connective tissues, articular cartilage does not contain blood vessels so it does not have the ability to regenerate and repair; therefore, materials that promote articular cartilage regeneration are important in tissue engineering studies. Mesenchymal stem cells (MSCs) can be found and isolated in different tissue including bone marrow, peripheral blood, umbilical cord blood, fat, synovial capsule, periodontal ligament, skin or solid mesenchymal tissues. The ability to transform into connective tissue system including hard bone, cartilage, fat and muscle tissue, which were cultured in several conditions that may induced to different tissues. Furthermore, functions of the platelets in medical research are not only coagulation and hemostasis, in the process of tissue repair and regeneration, the platelets can help regulate body metabolism, and promote tissue repair and healing. With the development of technology, we can get the concentrated platelet, platelet-rich fibrin releasate , which contains a variety of growth factor that maybe promote proliferation and differentiation of chondrocytes. In this study, an injectable platelet-rich fibrin releasate combine bone marrow stem cells was used for articular cartilage regeneration. New Zealand white rabbits were divided into four groups for treatment (n=6 per group): (1) control group; (2) injected platelet-rich fibrin releasate 1ml; (3) injected platelet-rich fibrin releasate 0.8 ml combine bone marrow stem cells 3×106 cells/0.2 ml medium; (4) injected bone marrow stem cells 3×106 cells/1 ml medium. After fourteen weeks, the bone appearance of healing was evaluated including coverage, color, cartilage surface defect edge and surface smooth. Histological investigation including cartilage surface, matrix, cell distribution, cell population viability, subchondral bone and cartilage mineralization. A statistically significant difference between the experimental and control groups was observed. The third group closest to the normal cartilage tissue structure, then followed by the second group. It was concluded that the promising experimental data are beginning to emerge in support of the use of platelet-rich fibrin relesate combine bone marrow stem cells for regenerative applications.

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tsung, Lia0 han, and 廖漢聰. "Combination of guided osteogenesis with autologous platelet-rich fibrin glue (PRFG) and mesenchymal stem cell for reconstruction of mandibular defects." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/38747058540196698334.

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碩士
長庚大學
顱顏口腔醫學研究所
95
Background: Mandibular bone defects commonly occur after trauma or cancer surgery. These defects are traditionally treated by autologous bone grafts to restore function and aesthetic appearance. However, harvesting autogenous bone grafts is traumatic and is associated with significant donor site morbidity and unpredictable resorption of the bone grafts. Guided tissue regeneration (GTR) and tissue-engineered bone regeneration are potential alternatives. This study examined whether a combination of autologous patelete-rich fibrin glue (PRFG) with mesenchymal stem cell and GTR with Medpor could act as an injectable osteogenic substitute and.whether this treatment yields faster new bone formation than Medpor alone or PRFG plus mesenchymal stem cell. Material and methods: Mesenchymal stem cells (MSCs) were harvested and isolated from the bone marrow of dog ilium. The cells were expanded in culture and mixed with autologous PRFG. Full thickness bony defects (1.5 x 1.5 cm) were created in the mandible angle of the dog. Admixtures of MSCs/autologous PRFG were placed into the bone defects, which were covered with a sheet of Medpor implant for guided-tissue regeneration in the experiment group. Treatments for bone defects in each group were as follows: Group 1: Medpor sheet + autologous PRFG/MSCs admixtures; Group 2: Autologous PRFG/MSCs admixtures; Group 3: Medpor sheet; Group 4: Control (empty defect). Bone regeneration was evaluated by computer tomography (CT) at 2 and 4 months postoperatively. The volume of regenerated bone was calculated by Analyse 7.0 software. All experimental animals were sacrificed at 4 months postoperatively. The Mandibles were harvested from all specimens, and the grafted sites were evaluated by gross, histological and X-ray examination. Results: In groups I and II, the CT, X-ray and histological examinations revealed new bone regeneration. However, more soft tissue ingrowth was noted in group II without medpor coverage as GTR. In groups III and IV, minimal bone formation was noted at the bone defect margins. The experimental results showed group I had 46.9±10.5% new bone growth at 2 months and 72.6±12.9% at 4 months, group II had 33.8±17.8% new bone growth at 2 months and 53.3±21.0% at 4 months, group III had 17.9±10.9% new bone growth at 2 months and 29.1±17.3% at 4 months and group IV had 11.0±5.07% new bone growth at 2 months and 15.1±5.85% new bone growth at 4 months. Conclusion: Autologous PRFG plus mesenchymal stem cell has good potential for bone regeneration. In combination with Medpor as GTR, bone regeneration is enhanced by preventing soft tissue ingrowth hindering bone regeneration.

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Jong, Yu-Tang, and 鐘毓棠. "The effect of Platelet-rich fibrin (PRF) extract and Adipose-derived stem cells (ADSCs) reconstruction on rabbit corneal damage reconstruction." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/59495347814739468158.

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碩士
國立屏東科技大學
生物科技系所
104
Platelet-rich fibrin (PRF), a biologic material isolated from centrifugal blood contains many cytokines and growth factor. Stem cells is undifferentiated cell which has potential to differentiate into different cell types. In 1986, Schermer found corneal stem cells in the limbus and the reports of stem cells reconstruct on cornea was increased. In our studies, we found PRF that can help stem cells express migration, proliferation, differentiation, angiogenesis, collagen synthesis and even regeneration of new tissue. In this study, cornea damage model were induced by immersion in NaOH. Cornea becomes nontransparent. The cornea’s epithelium and stroma were measured with microscope. Then, PRF was isolated from blood by cardiac puncture. The adipose-derived stem cells (ADSCs) was obtained from rabbit’s visceral fat. The damaged corneas were reconstructed with ADMSCs and PRF extract during 14 days treatment.

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Costa, Isa Andreia Canelas. "Avanço coronário com enxerto do conjuntivo versus membrana de fibrina plaquetária autólogo: revisão narrativa." Master's thesis, 2021. http://hdl.handle.net/10284/10593.

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Objetivos: O objetivo deste trabalho será realizar uma revisão narrativa, pretendendo comparar os resultados de PRF+CAF e PRF+CTG para o recobrimento radicular das revisões gengivais. Metodologia: Foi realizada uma pesquisa bibliográfica eletrónica através de dados Pub MED. A pesquisa foi efetuada até fevereiro de 2021 (256 artigos iniciais), tendo sido aplicados critérios de inclusão e de exclusão, nomeadamente com publicação < 5 anos e estudos relevantes em inglês, resultando um total de 189 artigos. Resultados: Da pesquisa bibliográfica após a aplicação dos critérios de inclusão/exclusão resultaram 5 artigos, que demonstraram ambas as técnicas usadas no recobrimento de recessões gengivais, bem como, os seus resultados clínicos, tendo estes sido analisados à posteriori. Conclusão: Aquando da aplicação da técnica de CAF+PRF obtiveram-se resultados clínicos favoráveis, sendo uma alternativa bastante promissora, nomeadamente, no que concerne ao quadro clínico pós-cirúrgico. No caso da técnica CAF+CTG, esta continua a ser o gold standard no recobrimento radicular propriamente dito, pela sua previsibilidade de resultados finais.
Goals: The objective of this work will be to carry out a narrative review, intending to compare the results of PRF+CAF and PRF+CTG for root coverage of gingival revisions. Methodology: An electronic bibliographic search was carried out using PubMed data, using the following keywords: gingival recession and platelet rich fibrin and connective tissue. The research was carried out until February 2021 (229 initial articles), and inclusion and exclusion criteria were applied, namely with publication <5 years and relevant studies in English, resulting in a total of 164 articles. Results: The literature search after the application of the inclusion/exclusion criteria resulted in 5 articles, which demonstrated both techniques used in the covering of gingival recessions, as well as their clinical results, which were analyzed a posteriori. Conclusion: When applying the CAF+PRF technique, favorable clinical results were obtained, being a very promising alternative, namely, with regard to the postsurgical clinical picture. In the case of the CAF+CTG technique, this continues to be the golden standard in root coverage itself, due to its predictability of final results.

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Lin, Ming-Cheng, and 林明正. "Functionalized bone-graft substitutes with platelet-rich fibrin and bone marrow-derived mesenchymal stem cells for enhanced bone tissue regeneration-with rabbit animal model." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/nnnx72.

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碩士
臺北醫學大學
醫療器材產業碩士專班
105
Functionalized bone-graft substitutes with platelet-rich fibrin and bone marrow-derived mesenchymal stem cells for enhanced bone tissue regeneration-with rabbit animal model. Porous bone grafting materials, which possess excellent bioactivity, biocompatibility and osteoconductivity, have played an important role in bone tissue regeneration. This study examined whether a combination of bone graft extender (BG), beta-tricalcium phosphate/hydroxyapatite (40/60) composite, with platelet-rich fibrin (PRF), bone marrow-derived mesenchymal stem cells (BMSCs) and/or gelatin-chondroitin-hyaluronan tri-copolymer scaffold could improve bone regeneration; and whether these treatments yield faster new bone formation following bone injury among the four treated groups that include (I) BG+PRF, (II) BG+PRF+BMSCs, (III) BG+scaffold, (IV) BG+scaffold+BMSCs. MSCs were harvested and isolated from the bone marrow of rabbits. Full-thickness bony defects (diameter 5.0 mm) were created in the bilateral mandible angles of the rabbit. Treatments for bone defect in each group were as follows: group I (n=3), BG 1.7 g and 0.75 cm3 of PRF; group II (n=4), BG 1.7 g and 0.75 cm3 of PRF/ 2x106 BMSCs admixtures; group III (n=3), BG 1.7 g and approximately 0.78 cm3 of scaffold; and group IV (n=4), BG 1.7 g and 0.78 cm3 of scaffold/ 2x106 BMSCs admixtures. The mandibles were harvested from all specimens at 3 months, and the grafted sites were evaluated by gross, histologic, and X-ray examination. Histologic examination revealed that the defect was repaired by typical bone tissue in groups II and IV, whereas only minimal bone formation with fibrous connection was observed in the groups I and III group. The best results were obtained with BG+PRF+BMSCs transplantation that had better bone union, formation and new bone position than BG+scaffold+BMSCs in the second, BG+Scaffold in the third, and BG+PRF in the last group. A statistically significant difference between the experimental and control groups was observed. Evidently, PRF plus BMSCs have good potential for bone regeneration and the technique describes a new alternative to bone grafting procedures for the treatment of critical sized bony lesions of the mandible.

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Chan, Ming-Yun, and 詹明澐. "The Effect of Platelet-rich Fibrin （PRF） Releasate Combined with Bone Marrow Stem Cells on Acute Kidney Injury by ischemia reperfusion with a Rat Model." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/27715562812479594001.

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碩士
國立臺灣大學
獸醫學研究所
104
Kidney disease affects millions of people in the world because of increasing incidence and prevalence and the high costs of treatment. Renal injury is mainly caused by toxic and ischemic injury. Renal ischemia-reperfusion occurs in different clinical situations including kidney transplantation, cardiopulmonary bypass, aortic bypass surgery, accidental trauma, sepsis, and hydronephrosis. Post ischemia-reperfusion kidney is characterized by the accumulation of extracellular matrix and usually results in a loss of function when normal parenchyma is replaced by fibrotic tissue. Abnormal kidney function will accumulate waste, toxins and useless moisture in the kidney tissue, may cause renal tubular dilation, glomerular sclerosis, proteinuria, or renal interstitial fibrosis, and finally lead to acute kidney injury (AKI). Stem cells are a kind of undifferentiated cells which may differentiate into other cell types. And they can also divide indefinitely into stem cell line, but may become cancer cells at about the tenth generation. We collect the bone marrow stem cells (BMSCs) for our research. Platelet-rich fibrin (PRF) is a natural source of growth factors, which contains a large amount of growth factors that can facilitate bone regeneration, Such as: TGFb-1 (Transforming growth factor), PDGF (platelet-derived growth factor) , VEGF (vascular endothelial growth factor) and Matrix proteins (thrombospondin-1, fibronectin, vitronectin). The growth factors secreted from PRF will cause cell transformation by promoting cell proliferation, matrix formation, bone formation and synthesis of collagen. We co-cultured rat bone marrow stem cells with platelet-rich fibrin releasate (PRFr), to investigate whether the release would improve stem cell proliferation in vitro or not. The results show that proliferation rate of the bone marrow stem cells which co-cultured with releasate were more rapid, and cell viability was much higher. Therefore, we deduced that platelet-rich fiber release solution have significant assistance on the growth and differentiation of bone marrow stem cells. To evaluate therapeutic efficacy of cell-based therapy in AKI: a bone marrow stem cells (BMSCs) and platelet-rich fibrin releasate (PRFr) were used for the treatment. An AKI was established with a rat model by ischemia reperfusion (IR). Transplantation of BMSCs, PRFr, and BMSCs + PRFr into IR rats for investigating the therapeutic potential for recovery of renal function. IR or sham operations were performed on SD rats at 10-weeks old, which were randomly divided into two parts: SHAM, and IR.6 rats in SHAM group was subjected to sham surgery, and 24 rats in IR group rats will accept four different treatments (1)Control group, injected 0.8ml PBS; (2)PRFr group, injected PRFr 0.8ml; (3)BMSCs group, injected BMSCs 2106 cells combine 0.8 ml PBS; (4)BMSCs + PRFr group, injected BMSCs 2106 cells combine 0.8 ml PRFr once a week for four weeks. At the firth month, urine was collected in one time a week; at the following three months, urine was collected in one time a month. At four months after surgery, the blood was taken for checking the value of blood urea nitrogen (BUN) and creatinine; the kidney was made into a Paraffin section for checking the effectiveness of treatments. Estimates based on preliminary data, not only the urinalysis (urine test) but also the serum analysis results indicated that BMSCs combined with PRFr group was significant lower than other groups. By three months after injection, the results of BMSCs combined with PRFr group were also measured in normal values, which performed better than those other groups. It was concluded that the promising experimental data are beginning to emerge in support of the use of MSCs combine PRFr for regenerative applications.

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Liu, Yu-Hao, and 劉宇豪. "Combination of Bone Marrow Derived Stem Cells and Dental Pulp Derived Stem Cells with Platelet-rich Fibrin Releasate (PRFr) to Repair and Regenerate Sciatic Nerves Injury." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/583eg3.

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碩士
國立臺灣大學
分子暨比較病理生物學研究所
104
The injury of sciatic nerves was a serious problem to the patients, such as costing money for treatment and even worse to change their life. However, there were many limitations in the traditional therapy and as a result a better treatment should be found out. Recently, the cell-based therapy has been focused to repair the broken-down nerves. In the mean times, using Platelet-rich fibrin releasate (PRFr) to repair damaged organs was an insight in Regenerative Medicine. Bone marrow derived stem cells (BMSCs) and Dental pulp derived stem cells (DPSCs) and PRFr were combined to treat with the animals. The 8 week old- injured nerve rats were separated into 6 groups. (1.) Control group, without any treatment; (2.) PRFr group,0.1 ml PRFr was injected; (3.) BMSCs group, 1x106 / 0.1 ml BMSCs were injected; (4.) DPSCs group, 1x106 / 0.1 ml DPSCs were injected; (5.) BMSC and PRFr group; (6.) DPSC and PRFr group, the rats were treated with 0.1 ml BMSCs and PRFr. Twelve weeks later, rats were sacrificed and the sciatic nerves regeneration was evaluated by gross and microscope. In gross, we measured the diameters of the regeneration sciatic nerves of every rats, then average every group and compared each other; in microscope, we calculate the axon density in the regeneration part of nerve fiber and average every group and compared. The result demonstrated that the combined treatment has significant impact than other groups. Following that was stem cells (BMSCs, DPSCs) treatment. However, the PRFr group was not efficiency as our expect but it still reached significant than the control group. In conclusion, cell based therapy may provide a new way to the nerve regeneration.

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Cardoso, Cláudia Sofia Pinto e. Silva. "Coágulo A+-PRF convencional versus coágulo A+-PRF com protocolo PomPac: estudo comparativo do comprimento." Master's thesis, 2021. http://hdl.handle.net/10284/10377.

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Objetivo: Este estudo teve por objetivo compreender se a temperatura a que a colheita sanguínea é acondicionada no protocolo Advanced Plus-Platelet Rich Fibrin (A+-PRF), interfere nos resultados dimensionais dos coágulos. Materiais e Métodos: Dez voluntários, sem histórico de toma de anti-coagulantes e com intervenções cirúrgicas com tratamento com A+-PRF, foram submetidos a colheita de quatro tubos de sangue. Dois tubos colhidos sob protocolo convencional e outros dois sob protocolo PomPac. Após colheita procedeu-se à produção de coágulos segundo o protocolo de A+-PRF, previamente descrito na literatura (Ghanaati et al, 2014). Os coágulos (4x10 = 40) foram submetidos a registo de dimensões de comprimento. Posteriormente foi realizada análise estatística dos dados. Resultados: A dimensão média dos coágulos provenientes do protocolo A+-PRF convencional foi de 29,10 milímetros (mm) e os provenientes do protocolo com Pompac foi de 34,35 mm (p<0,001; n=20). Para a dimensão máxima registou-se para o protocolo A+-PRF o valor de 35 mm e para o Protocolo A+-PRF com Pompac 41mm (p<0,001; n=20). Conclusão: Este estudo permitiu concluir que o protocolo A+-PRF com Pompac permite obter coágulos com comprimento máximo e médio mais elevados que os obtidos com o protocolo A+-PRF convencional. Estes fatos fazem do protocolo A+-PRF com Pompac uma melhor escolha em detrimento do protocolo A+-PRF convencional no que respeita à quantidade de biomaterial disponível.
Purpose: This study aimed to understand the temperature at which the blood collection is stored in the Advanced Plus-Platelet Rich Fibrin (A+ -PRF) protocol, interferes with the dimensional results of clots. Materials and Methods: Ten volunteers, with no history of anticoagulants medication and undergoing surgical interventions with treatment with A+-PRF, underwent collection of four tubes of blood. Two tubes were collected under conventional protocol and another two under PomPac protocol. After collection, clots were produced according to the A+-PRF protocol, previously described in the literature (Ghanaati et al, 2014). The clots (4x10 = 40) were submitted to length dimension recording. Subsequently, statistical analysis of the data was performed. Results: The mean dimension of clots from the conventional A+-PRF protocol was 29,10 millimeter (mm) and those from the Pompac protocol was 34,35 mm (p<0.001; n=20). For the maximum dimension, the value of 35 mm was recorded for the A+-PRF protocol and 41 mm for the A+-PRF protocol with Pompac (p<0.001; n=20). Conclusion: This study allowed us to conclude that the A+-PRF protocol with Pompac allows to obtain clots with higher maximum and average length than those obtained with the conventional A+-PRF protocol. These facts make the A+-PRF protocol with Pompac a better choice over the conventional A+-PRF protocol in terms of the amount of biomaterial available.

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Otero, Ada Isis Pelaez. "Sinus lift associated with second generation of autologous platelet concentrates : a systematic review." Master's thesis, 2021. http://hdl.handle.net/10400.14/34807.

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Introduction. The purpose of this systematic review was lift to analyze the available literature of clinical studies involving sinus lifting and comparing the efficiency of 2 nd autologous platelet concentrates (APC) generation related to its effects in bone gain and to clarify the regenerative efficacy of APC in sinus lift procedure, whether alone or as a coadjutant to other bone graft materials. Material and methods. This systematic review was conducted following the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) guidelines (1) with the focused question being determined according to the Population, Intervention, Comparison and Outcome, Follow-up (PICO) strategy.⁠ The protocol for this systematic review was registered on PROSPERO. The focused question for the present review was as follows: “In clinical studies with patients needing a maxillary sinus lift (P), does the use of ACPs either alone (I) or in conjunction with other biomaterials (C) improve the clinical outcome associated with bone gain and density (O), with at least 3 months of follow-up? An electronic search was conducted by two reviewers (A.I.P. and G.V.O.F.), independently, either electronic as manual search, through MEDLINE (PubMed), Science Direct, and Scopus databases with a platformspecific search strategy combining terms such as “sinus floor elevation”, “sinus augmentation”, “platelet-rich fibrin”, “second generation”, or “PRF”, and variables. Results. A total of 443 articles were obtained from the electronic database search, 67 on PubMed, 129 on Science Direct, and 247 on Scopus. An additional publication was considered from the manual search through the references of the included articles. After a full-text review, a total of 16 articles met all the criteria and were included in this review. Conclusion. Through the use of APCs in sinus augmentation the time required for bone graft maturation may be significantly reduced thus allowing sooner implant insertion, however overall long-term bone development did not show any significant difference.
Introdução. O objetivo desta revisão sistemática é analisar a literatura disponível em estudos clínicos envolvendo levantamento do seio e comparar a eficiência da 2ª geração de concentrados autogolos plaquetarios APC relacionada aos seus efeitos no ganho ósseo e esclarecer sua eficácia regenerativa no procedimento de levantamento do seio, seja sozinho ou como coadjuvante de outros biomateriais. Material e métodos. A questão em foco para a presente revisão foi a seguinte: “Em estudos clínicos com pacientes que precisam de elevação do seio maxilar (P), o uso de ACPs, isoladamente (I) ou em conjunto com outros biomateriais (C), melhora o resultado clínico associado com ganho e densidade óssea (O), com no mínimo 3 meses de seguimento ? Uma busca eletrónica foi conduzida por dois revisores (A.I.P e G.V.O.F), de forma independente, tanto electrónica quanto manual, por meio de bancos de dados MEDLINE (PubMed), ScienceDirect e Scopus com uma estratégia de busca específica da plataforma combinando termos como "elevação do assoalho do seio", “Aumento do seio”, “fibrina rica em plaquetas”, “segunda geração” ou “PRF” e variáveis. Resultados. Um total de 443 artigos foi obtido a partir da busca em banco de dados eletrónico, 67 no PubMed, 129 no Science Direct e 247 no Scopus. Uma publicação adicional foi considerada a partir da pesquisa manual nas referências dos artigos incluídos. Após a revisão do texto completo, um total de 16 artigos preencheram todos os critérios e foram incluídos nesta revisão. Conclusão. Através do uso de APCs no aumento do seio, o tempo necessário para a maturação do enxerto ósseo pode ser significativamente reduzido, permitindo a inserção mais rápida do implante, no entanto, o desenvolvimento ósseo geral a longo prazo não mostrou nenhuma diferença significativa.

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Vilela, Beatriz Figueira. "Retalho de avanço coronal utilizando membrana de PRF versus enxerto de tecido conjuntivo: revisão narrativa." Master's thesis, 2021. http://hdl.handle.net/10284/10825.

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Esta revisão tem como principal objetivo avaliar o prognóstico de um retalho de avanço coronal utilizando uma membrana PRF comparativamente a um enxerto de tecido conjuntivo. Assim, são analisadas diversas variáveis, tais como profundidade de recessão, profundidade de sondagem, índice de placa, largura da recessão, nível de inserção clínica, altura de tecido queratinizado, espessura de tecido queratinizado, recobrimento radicular parcial e completo e desconforto pós operatório. Foram incluídos artigos em inglês, publicados entre 2015 e 2020 foram procurados em três bases de dados distintas (PubMed, Science Direct e Scielo). Apenas foram considerados artigos de revisão sistemática, meta-análises ou ensaios clínicos randomizados que fossem realizados na espécie humana.
The main objective of this review is to evaluate the prognosis of coronal advanced flap using a PRF membrane comparared to a connective tissue graft through the analysis of several variables: recession depth, probing depht, plaque índex, recession width, clinical attachment level, keratinised tissue width, keratinised tissue thickness, parcial and complete root coverage and postoperative discomfort. Articles in english, published between 2015 and 2020 being searched in three databases (PubMed, Science Direct, Scielo) with only systematic reviews, meta-analysis and random clinical trials performed on humans were considered.

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Dissertations / Theses on the topic 'Platelet-rich fibrin'

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