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Journal articles on the topic 'PLC'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Ngoc My, Tran Thi. "ISOLATING THE MONOKARYON COLLECTION OF Pleurotus spp." Vietnam Journal of Science and Technology 55, no. 1A (2018): 73. http://dx.doi.org/10.15625/2525-2518/55/1a/12384.

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Pleurotus spp. is one of the most important cultivated mushrooms in the world by their nutrition and medicinal property. In Vietnam, although many Pleurotus species are popularly cultivated, but most of them are imported. These strains are easily degenerated when they are cultivated in large scale, because they are difficult to adapt to the environment of Vietnam. This research aims to construct the monokaryon collection of Pleurotus spp. with details of taxonomy, genetic diversity, cultivating traits, monokaryotic mating types focused on Pleurotus strains with the high yield, genetic stable a

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2

Cheeseman, Keylon L., Takehiko Ueyama, Tanya M. Michaud та ін. "Targeting of Protein Kinase C-ϵ during Fcγ Receptor-dependent Phagocytosis Requires the ϵC1B Domain and Phospholipase C-γ1". Molecular Biology of the Cell 17, № 2 (2006): 799–813. http://dx.doi.org/10.1091/mbc.e04-12-1100.

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Protein kinase C-ϵ (PKC-ϵ) translocates to phagosomes and promotes uptake of IgG-opsonized targets. To identify the regions responsible for this concentration, green fluorescent protein (GFP)-protein kinase C-ϵ mutants were tracked during phagocytosis and in response to exogenous lipids. Deletion of the diacylglycerol (DAG)-binding ϵC1 and ϵC1B domains, or the ϵC1B point mutant ϵC259G, decreased accumulation at phagosomes and membrane translocation in response to exogenous DAG. Quantitation of GFP revealed that ϵC259G, ϵC1, and ϵC1B accumulation at phagosomes was significantly less than that o

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3

DIJK, Marc C. M. van, Francisco J. G. MURIANA, Paul C. J. van der HOEVEN та ін. "Diacylglycerol generated by exogenous phospholipase C activates the mitogen-activated protein kinase pathway independent of Ras- and phorbol ester-sensitive protein kinase C: dependence on protein kinase C-ζ". Biochemical Journal 323, № 3 (1997): 693–99. http://dx.doi.org/10.1042/bj3230693.

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The role of diacylglycerol (DG) formation from phosphatidylcholine in mitogenic signal transduction is poorly understood. We have generated this lipid at the plasma membrane by treating Rat-1 fibroblasts with bacterial phosphatidylcholine-specific phospholipase C (PC-PLC). This treatment leads to activation of mitogen-activated protein kinase (MAPK). However, unlike platelet-derived growth factor (PDGF) or epidermal growth factor (EGF), PC-PLC fails to activate Ras and to induce DNA synthesis, and activates MAPK only transiently (< 45 min). Down-regulation of protein kinase C (PKC) -α, -Δ a

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4

Xu, X. X., T. G. Tessner, C. O. Rock, and S. Jackowski. "Phosphatidylcholine hydrolysis and c-myc expression are in collaborating mitogenic pathways activated by colony-stimulating factor 1." Molecular and Cellular Biology 13, no. 3 (1993): 1522–33. http://dx.doi.org/10.1128/mcb.13.3.1522-1533.1993.

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Stimulation of diglyceride production via phospholipase C (PLC) hydrolysis of phosphatidylcholine was an early event in the mitogenic action of colony-stimulating factor 1 (CSF-1) in the murine macrophage cell line BAC1.2F5 and was followed by a second phase of diglyceride production that persisted throughout the G1 phase of the cell cycle. Addition of phosphatidylcholine-specific PLC (PC-PLC) from Bacillus cereus to the medium of quiescent cells raised the intracellular diglyceride concentration and stimulated [3H]thymidine incorporation, although PC-PLC did not support continuous proliferati

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Xu, X. X., T. G. Tessner, C. O. Rock, and S. Jackowski. "Phosphatidylcholine hydrolysis and c-myc expression are in collaborating mitogenic pathways activated by colony-stimulating factor 1." Molecular and Cellular Biology 13, no. 3 (1993): 1522–33. http://dx.doi.org/10.1128/mcb.13.3.1522.

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Stimulation of diglyceride production via phospholipase C (PLC) hydrolysis of phosphatidylcholine was an early event in the mitogenic action of colony-stimulating factor 1 (CSF-1) in the murine macrophage cell line BAC1.2F5 and was followed by a second phase of diglyceride production that persisted throughout the G1 phase of the cell cycle. Addition of phosphatidylcholine-specific PLC (PC-PLC) from Bacillus cereus to the medium of quiescent cells raised the intracellular diglyceride concentration and stimulated [3H]thymidine incorporation, although PC-PLC did not support continuous proliferati

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6

Zhao, Han, Jiayu Zhang, Dengliang Li та ін. "miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway". PLOS ONE 20, № 1 (2025): e0316074. https://doi.org/10.1371/journal.pone.0316074.

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Porcine epidemic diarrhea virus (PEDV) is a significant pathogen affecting swine, causing severe economic losses worldwide. This study explores the regulatory role of miRNA-328-3p to ZO-1 expression and its impact on PEDV proliferation via the PLC-β1-PKC pathway in IPEC-J2 cells. We found that miRNA-328-3p can target ZO-1, influencing its expression and subsequently affecting the integrity of tight junctions in the cells. Overexpression of PLC-β1, combined with miRNA-328-3p silencing, enhanced ZO-1 expression, while PLC-β1 knockdown combined with miRNA-328-3p overexpression inhibited ZO-1 expr

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7

FILTZ, Theresa M., Michelle L. CUNNINGHAM, Kara J. STANIG, Andrew PATERSON та T. Kendall HARDEN. "Phosphorylation by protein kinase C decreases catalytic activity of avian phospholipase C-β". Biochemical Journal 338, № 2 (1999): 257–64. http://dx.doi.org/10.1042/bj3380257.

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The potential role of protein kinase C (PKC)-promoted phosphorylation has been examined in the G-protein-regulated inositol lipid signalling pathway. Incubation of [32P]Pi-labelled turkey erythrocytes with either the P2Y1 receptor agonist 2-methylthioadenosine triphosphate (2MeSATP) or with PMA resulted in a marked increase in incorporation of 32P into the G-protein-activated phospholipase C PLC-βT. Purified PLC-βT also was phosphorylated by PKC in vitro to a stoichiometry (mean±S.E.M.) of 1.06±0.2 mol of phosphate/mol of PLC-βT. Phosphorylation by PKC was isoenzyme-specific because, under ide

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8

Bhattarai, Mohan, Gerardo Morell, and Ram S. Katiyar. "Rare Earth Doped Lead Zirconate Titanate Thin Films for Memory and Power Device Applications." ECS Meeting Abstracts MA2023-01, no. 30 (2023): 1817. http://dx.doi.org/10.1149/ma2023-01301817mtgabs.

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We fabricated thin films of (PbZr0.53Ti0.47)O3 (PZT) and (PbZr0.53Ti0.47)0.90(LaxSc1-x)0.10O3 where; x= 0, 0.2, 0.4, 0.6 and 0.8 denoted as [abbr. PL10x] PL0, PL2, PL4, PL6, and PL8 respectively on the LSMO buffer layer (bottom electrode) by using a PLD technique. The X-ray diffraction peaks show a single-phase perovskite structure of thin films with a preferred (100) orientation that coincides with the MgO and has tetragonal phase symmetry (P4mm). Pt electrodes were deposited at the top of thin films as top electrodes. We achieved higher dielectric permittivity (ε’) for PL0 and PL6 compared t

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9

LITOSCH, Irene. "G-protein βγ subunits antagonize protein kinase C-dependent phosphorylation and inhibition of phospholipase C-β1". Biochemical Journal 326, № 3 (1997): 701–7. http://dx.doi.org/10.1042/bj3260701.

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Protein kinase C (PKC) isoforms phosphorylated phospholipase C-β1 (PLC-β1) in vitro as follows: PKCα ≫ PKCϵ; not PKCζ. PLC-β3 was not phosphorylated by PKCα. G-protein βγ subunits inhibited the PKCα phosphorylation of PLC-β1 in a concentration-dependent manner. Half-maximal inhibition occurred with 500 nM βγ. G-protein βγ subunits also antagonized the PKCα-mediated inhibition of PLC-β1 enzymic activity. PKCα, in turn, inhibited the stimulation of PLC-β1 activity by βγ. There was little effect of PKCα on the stimulation of PLC-β1 by αq/11–guanosine 5′[γ-thio]triphosphate (GTP[S]). These finding

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10

Huynh, Minh Q., Thomas Wuendisch, Annette Ramaswamy та Andreas Neubauer. "Activation of Phospholipase C γ 2 in Lymphoma Cells." Blood 106, № 11 (2005): 1220. http://dx.doi.org/10.1182/blood.v106.11.1220.1220.

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Abstract The Nf-κB signaling pathway is important for lymphoma survival and cell proliferation. In recent immunohistological studies, the B-cell receptor (BCR), Src kinases and PLC γ 2 were shown to be highly expressed in primary mediastinal B-cell lymphoma and lymphocyte predominant Hodgkin lymphoma, but not in classical Hodgkin lymphoma. Activation of PLC γ 2 is a crucial step in regulating Nf-κB through protein kinase C (PKC). The PLC-PKC cascade also activates the Ras-Raf-Erk signaling pathway by recruiting GRP3 to the cell membrane. These signaling cascades are well studied in physiologic

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VAN DIJK, Marc C. M., Henk HILKMANN та Wim J. VAN BLITTERSWIJK. "Platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase C, protein kinase C-ζ and Raf-1". Biochemical Journal 325, № 2 (1997): 303–7. http://dx.doi.org/10.1042/bj3250303.

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The mechanism of Raf-1 activation by platelet-derived growth factor (PDGF) is poorly defined. We previously reported that, in Rat-1 fibroblasts, PDGF activates a phosphatidylcholine-specific phospholipase C (PC-PLC) and that the product, diacylglycerol, somehow activates protein kinase C-ζ (PKC-ζ). Both PC-PLC and PKC-ζ activities were required for PDGF activation of mitogen-activated protein kinase (MAPK). Now we report that MAPK activation by exogenous PC-PLC depends on Raf-1 activation. PKC-ζ co-immunoprecipitates with, phoshorylates and activates Raf-1, suggesting that in the PDGF- and PC-

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12

Jiang, Quan, Mulan He, Xinyan Wang та Anderson O. L. Wong. "Grass carp somatolactin: II. Pharmacological study on postreceptor signaling mechanisms for PACAP-induced somatolactin-α and -β gene expression". American Journal of Physiology-Endocrinology and Metabolism 295, № 2 (2008): E477—E490. http://dx.doi.org/10.1152/ajpendo.90386.2008.

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Somatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. However, the signal transduction mechanisms responsible for SL expression are still largely unknown. Using grass carp as an animal model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL gene expression at the pituitary level. In primary cultures of grass carp pituitary cells, SLα and SLβ mRNA levels could be elevated by PACAP via activation of PAC-I receptors. With the use of a pharmacological approach, the AC/cAMP/

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13

Yu, Peirong, Qian Chen, Zuoliang Xiao, Karen Harnett, Piero Biancani, and Jose Behar. "Signal transduction pathways mediating CCK-induced gallbladder muscle contraction." American Journal of Physiology-Gastrointestinal and Liver Physiology 275, no. 2 (1998): G203—G211. http://dx.doi.org/10.1152/ajpgi.1998.275.2.g203.

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The signal transduction that mediates CCK-induced contraction of gallbladder muscle was investigated in the cat. Contraction was measured by scanning micrometry in single muscle cells isolated enzymatically with collagenase. Production ofd- myo-inositol 1,4,5-trisphosphate (IP3) and sn-1,2-diacylglycerol (DAG) was quantitated using HPLC and TLC, respectively. Protein kinase C (PKC) activity was determined by measuring the phosphorylation of a specific substrate peptide from myelin basic protein, Ac-MBP-(4—14). CCK-induced contraction was blocked by incubation in strontium medium, pertussis tox

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14

Norris, Richard, Christian Kopkow, and Michael James McNicholas. "Interpretations of the dial test should be reconsidered. A diagnostic accuracy study reporting sensitivity, specificity, predictive values and likelihood ratios." Journal of ISAKOS: Joint Disorders & Orthopaedic Sports Medicine 3, no. 4 (2018): 198–204. http://dx.doi.org/10.1136/jisakos-2018-000197.

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ObjectivesTo determine the accuracy of the dial test, used alone and in combination with additional clinical tests, in the diagnosis of an isolated posterolateral corner (PLC) injury, combined PLC-posterior cruciate ligament (PCL) injury or medial knee injury.MethodsA retrospective analysis of consecutive patients who underwent arthroscopic and/or open knee ligament reconstruction surgery was conducted. The dial test was performed in an outpatient’s clinic as part of a routine knee examination. Examination under anaesthetic and intraoperative findings were used as the reference standard test t

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15

Son, N. T., D. Shafizadeh, T. Ohshima, and I. G. Ivanov. "Modified divacancies in 4H-SiC." Journal of Applied Physics 132, no. 2 (2022): 025703. http://dx.doi.org/10.1063/5.0099017.

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Divacancies near or at lattice defects in SiC, the PL5–PL7 photoluminescence centers, are known to have more favorable optical and spin properties for applications in quantum technology compared to the usual divacancies. These centers were previously predicted to be divacancies near stacking faults. Using electron paramagnetic resonance, we observe PL5, PL6, and four other divacancy-like centers, labeled PLa–PLd, in electron-irradiated high-purity semi-insulating (HPSI) 4H-SiC. From the observed fine-structure D-tensors, we show that these centers including PL6, which has so far been believed

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16

BAI, Xiao-Chun, Fan DENG, An-Ling LIU та ін. "Phospholipase C-γ1 is required for cell survival in oxidative stress by protein kinase C". Biochemical Journal 363, № 2 (2002): 395–401. http://dx.doi.org/10.1042/bj3630395.

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Phospholipase C-γ1 (PLC-γ1) activation has been reported to enhance cell survival during the cellular response to oxidative stress. We studied the role of protein kinase C (PKC) pathways in mediating PLC-γ1 survival signalling in oxidative stress by using mouse embryonic fibroblasts genetically deficient in PLC-γ1 (Plcg1−/−) and its wild type (Plcg1+/+). PLC-γ1 was activated by H2O2 treatment in a dose- and time-dependent manner. Activation of PKC was also markedly increased in both cell lines treated with H2O2 (1–5mM), but with low doses (50–200μM), PKC activation was considerably decreased i

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17

Moatshe, Gilbert, Jorge Chahla, Alex W. Brady, et al. "The Influence of Graft Tensioning Sequence on Tibiofemoral Orientation During Bicruciate and Posterolateral Corner Knee Ligament Reconstruction: A Biomechanical Study." American Journal of Sports Medicine 46, no. 8 (2018): 1863–69. http://dx.doi.org/10.1177/0363546517751917.

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Background: During multiple knee ligament reconstructions, the graft tensioning order may influence the final tibiofemoral orientation and corresponding knee kinematics. Nonanatomic tibiofemoral orientation may result in residual knee instability, altered joint loading, and an increased propensity for graft failure. Purpose: To biomechanically evaluate the effect of different graft tensioning sequences on knee tibiofemoral orientation after multiple knee ligament reconstructions in a bicruciate ligament (anterior cruciate ligament [ACL] and posterior cruciate ligament [PCL]) with a posterolate

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18

Mangat, Rabban, Tushi Singal, Naranjan S. Dhalla та Paramjit S. Tappia. "Inhibition of phospholipase C-γ1augments the decrease in cardiomyocyte viability by H2O2". American Journal of Physiology-Heart and Circulatory Physiology 291, № 2 (2006): H854—H860. http://dx.doi.org/10.1152/ajpheart.01205.2005.

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The present study was conducted to examine the role of a major cardiac phospholipase C (PLC) isozyme, PLC-γ1, in cardiomyocytes during oxidative stress. Left ventricular cardiomyocytes were isolated by collagenase digestion from adult male Sprague-Dawley rats (250–300 g) and treated with 20, 50, and 100 μM H2O2for 15 min. A concentration-dependent (up to 50 μM) increase in the mRNA level and membrane protein content of PLC-γ1was observed with H2O2treatment. Furthermore, PLC-γ1was activated in response to H2O2, as revealed by an increase in the phosphorylation of its tyrosine residues. There wa

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Wadsworth, Sandra J., and Howard Goldfine. "Mobilization of Protein Kinase C in Macrophages Induced by Listeria monocytogenes Affects Its Internalization and Escape from the Phagosome." Infection and Immunity 70, no. 8 (2002): 4650–60. http://dx.doi.org/10.1128/iai.70.8.4650-4660.2002.

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ABSTRACT Listeriolysin O (LLO) and a phosphatidylinositol-specific phospholipase C (PI-PLC) are known virulence factors of Listeria monocytogenes in both tissue cultures and the murine model of infection. LLO is a member of a family of pore-forming cholesterol-dependent cytotoxins and is known to play an essential role in escape from the primary phagocytic vacuole of macrophages. PI-PLC plays an accessory role, in that PI-PLC mutants are partially defective in escape. We have shown that both of these molecules are essential for initiating rapid increases in the calcium level in the J774 murine

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20

Wang, Zhixiang, Stefan Glück, Lianfeng Zhang та Michael F. Moran. "Requirement for Phospholipase C-γ1 Enzymatic Activity in Growth Factor-Induced Mitogenesis". Molecular and Cellular Biology 18, № 1 (1998): 590–97. http://dx.doi.org/10.1128/mcb.18.1.590.

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ABSTRACT The cytoplasmic regions of the receptors for epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) bind and activate phospholipase C-γ1 (PLC-γ1) and other signaling proteins in response to ligand binding outside the cell. Receptor binding by PLC-γ1 is a function of its SH2 domains and is required for growth factor-induced cell cycle progression into the S phase. Microinjection into MDCK epithelial cells and NIH 3T3 fibroblasts of a polypeptide corresponding to the noncatalytic SH2-SH2-SH3 domains of PLC-γ1 (PLC-γ1 SH2-SH2-SH3) blocked growth factor-induced S-phase en

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Brown, Jessica, Ben Grayson, Joanna C. Neill, Michael Harte, Mark J. Wall, and Richard T. Ngomba. "Oscillatory Deficits in the Sub-Chronic PCP Rat Model for Schizophrenia Are Reversed by mGlu5 Receptor-Positive Allosteric Modulators VU0409551 and VU0360172." Cells 12, no. 6 (2023): 919. http://dx.doi.org/10.3390/cells12060919.

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The cognitive deficits of schizophrenia are linked to imbalanced excitatory and inhibitory signalling in the prefrontal cortex (PFC), disrupting gamma oscillations. We previously demonstrated that two mGlu5 receptor-positive allosteric modulators (PAMs), VU0409551 and VU0360172, restore cognitive deficits in the sub-chronic PCP (scPCP) rodent model for schizophrenia via distinct changes in PFC intracellular signalling molecules. Here, we have assessed ex vivo gamma oscillatory activity in PFC slices from scPCP rats and investigated the effects of VU0409551 and VU0360172 upon oscillatory power.

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22

Martin, K. J., C. L. McConkey, A. K. Jacob, E. A. Gonzalez, M. Khan, and J. J. Baldassare. "Effect of U-73,122, an inhibitor of phospholipase C, on actions of parathyroid hormone in opossum kidney cells." American Journal of Physiology-Renal Physiology 266, no. 2 (1994): F254—F258. http://dx.doi.org/10.1152/ajprenal.1994.266.2.f254.

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The relative roles of the adenylate cyclase-protein kinase A system (AC-PKA), the phospholipase C-protein kinase C system (PLC-PKC), and increases in cytosolic calcium in mediating the final actions of parathyroid hormone (PTH) remain ill defined. Although an important role for the PLC-PKC system in the regulation of phosphate transport in response to PTH has been suggested, previous studies from our laboratory and others, in OK cells, have emphasized the major role of AC-PKA. The present studies were designed to dissociate the second messengers for PTH by using an inhibitor of PLC (U-73,122).

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Barnett, R. L., L. Ruffini, L. Ramsammy, R. Pasmantier, M. M. Friedlaender, and E. P. Nord. "Angiotensin-mediated phosphatidylcholine hydrolysis and protein kinase C activation in mesangial cells." American Journal of Physiology-Cell Physiology 265, no. 4 (1993): C1100—C1108. http://dx.doi.org/10.1152/ajpcell.1993.265.4.c1100.

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Angiotensin II (ANG II) in mesangial cells (MC) promotes phosphatidylinositol (PI) hydrolysis resulting in diacylglycerol (DAG)-mediated increases in protein kinase C (PKC) activity. The paucity of MC inositol lipid prompted us to consider whether phosphatidylcholine (PC) could sustain DAG formation. ANG II released choline and increased phosphatidylethanol (PEt) via PC-phospholipase D (PC-PLD). ANG II also stimulated phosphorylcholine consequent to PC-phospholipase C (PC-PLC) activation. ANG II-mediated PC hydrolysis augmented DAG for 30 min. PC breakdown was influenced by extracellular Ca2+,

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Aryana, I. Gusti Ngurah Wien, and Putu Kermawan. "Posterior cruciate ligament and posterolateral corner reconstruction in patient with multiple ligament injury of the knee: a case report." International Journal of Research in Medical Sciences 8, no. 9 (2020): 3374. http://dx.doi.org/10.18203/2320-6012.ijrms20203696.

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The treatment of posterolateral corner (PLC) knee injuries has always been a challenging topic due to the low healing capacity of PLC injuries. Authors performed posterior cruciate ligament reconstruction using semitendinosus tendon graft and reconstruction procedure of PLC using free gracilis tendon graft with LaPrade technique in a patient with multiple ligament injury of the knee. A 36-years-old male patient complained of pain on his right knee. On physical examination, the posterior drawer test, dial test, and varus stress test were positive. Magnetic resonance imaging (MRI) on right knee

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Poussin, Mathilde A., and Howard Goldfine. "Involvement of Listeria monocytogenes Phosphatidylinositol-Specific Phospholipase C and Host Protein Kinase C in Permeabilization of the Macrophage Phagosome." Infection and Immunity 73, no. 7 (2005): 4410–13. http://dx.doi.org/10.1128/iai.73.7.4410-4413.2005.

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ABSTRACT We have previously shown that phosphatidylinositol-specific phospholipase C (PI-PLC) produced by Listeria monocytogenes activates a host protein kinase C (PKC) cascade which promotes escape of the bacterium from a macrophage-like cell phagosome. Here, we provide evidence linking bacterial PI-PLC and host PKC β to phagosome permeabilization, which precedes escape.

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Suzuki, M., T. Asplund, H. Yamashita, C. H. Heldin та P. Heldin. "Stimulation of hyaluronan biosynthesis by platelet-derived growth factor-BB and transforming growth factor-β1 involves activation of protein kinase C". Biochemical Journal 307, № 3 (1995): 817–21. http://dx.doi.org/10.1042/bj3070817.

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The intracellular signal transduction pathways that mediate the stimulatory effects of platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-beta on hyaluronan biosynthesis in human fibroblasts were investigated. The stimulatory effects of both PDGF-BB and TGF-beta 1 were dependent on protein kinase C (PKC), since the PKC inhibitor calphostin C inhibited the stimulation by the growth factors. Direct activation of PKC by phorbol 12-myristate 13-acetate (PMA) also stimulated hyaluronan production, and the combination of either PDGF-BB or TGF-beta 1 and PMA gave an increas

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Abu-Mukh, Assala, Seungyup Lee, Hye Chang Rhim, and Ki-Mo Jang. "Exploring the Posterolateral Corner of the Knee Joint: A Detailed Review of Recent Literature." Journal of Clinical Medicine 14, no. 5 (2025): 1549. https://doi.org/10.3390/jcm14051549.

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One of the least understood and most elaborate and neglected knee stabilizers is the posterolateral corner (PLC) complex. PLC injury is associated with a high risk of re-injury, early athletic career termination, instability, progressive osteoarthritis, and a high risk of artificial knee replacement. The growing focus on the PLC, along with various recent anatomical and biomechanical studies, has provided further insights into the anatomy and function of posterolateral structures in knee stabilization and kinematics. The PLC should be considered as a functional unit, not only an anatomical uni

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Chen, P., H. Xie, and A. Wells. "Mitogenic signaling from the egf receptor is attenuated by a phospholipase C-gamma/protein kinase C feedback mechanism." Molecular Biology of the Cell 7, no. 6 (1996): 871–81. http://dx.doi.org/10.1091/mbc.7.6.871.

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We recently demonstrated that epidermal growth factor receptor (EGFR)-mediated signaling of cell motility and mitogenesis diverge at the immediate post-receptor level. How these two mutually exclusive cell responses cross-communicate is not known. We investigated a possible role for a phospholipase C (PLC)-dependent feedback mechanism that attenuates EGF-induced mitogenesis. Inhibition of PLC gamma activation by U73122 (1 microM) augmented the EGF-induced [3H]thymidine incorporation by 23-55% in two transduced NR6 fibroblast lines expressing motility-responsive EGFR; increased cell division an

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Moatshe, Gilbert, Jorge Chahla, Alex Brady, et al. "The Influence of Graft Tensioning Sequence on Tibiofemoral Orientation during Bicruciate and Posterolateral Corner Knee Ligament Reconstruction: A Biomechanical Study." Orthopaedic Journal of Sports Medicine 6, no. 7_suppl4 (2018): 2325967118S0006. http://dx.doi.org/10.1177/2325967118s00066.

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Objectives: During a multiple knee ligament reconstruction, the graft tensioning order may influence the final tibiofemoral orientation and corresponding knee kinematics. Therefore, the objective of this study was to biomechanically evaluate the effect of different graft tensioning sequences on knee tibiofemoral orientation following multiple knee ligament reconstruction in a bicruciate ligament (anterior cruciate ligament [ACL] and posterior cruciate ligament [PCL]) with posterolateral corner [PLC] injured knee. Methods: Ten non-paired, fresh-frozen human cadaveric knees were utilized for thi

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PESO, Luis del, Luisa LUCAS, Pilar ESTEVE, and Juan Carlos LACAL. "Activation of phospholipase D by growth factors and oncogenes in murine fibroblasts follow alternative but cross-talking pathways." Biochemical Journal 322, no. 2 (1997): 519–28. http://dx.doi.org/10.1042/bj3220519.

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Phospholipase D (PLD) is activated by a variety of stimuli, including mitogenic stimulation by growth factors and oncogene transformation. Activation of PLD by growth factors requires protein kinase C (PKC) since depletion of the enzyme by down-regulation or direct inhibition by specific drugs completely abrogates this effect. Transformation by the rasand srconcogenes is also associated with an increase in basal PLD activity. However, this effect is not dependent on PKC, suggesting that growth factors and oncogenes may activate PLD by two independent mechanisms. Here we demonstrate that activa

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Karpovich, V. V., S. V. Churashov, and V. F. Chernysh. "Experimental study of biodegradation of synthetic polymer matrices as promising scaffolds for limbal stem cells." Modern technologies in ophtalmology, no. 4 (September 29, 2023): 15–20. http://dx.doi.org/10.25276/2312-4911-2023-4-15-20.

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Aim. To study in experimental conditions and conduct a comparative assessment of the terms of biodegradation of three different types of synthetic polyester matrices as promising carriers of limbal stem cells. Material and methods. We performed matrices from poly(lactide-glycolide) (PLG), poly(lactide-caprolactone) (PLC) and poly(ε-caprolactone) (PCL). On the basis of the S.M. Kirov Military Medical Academy (St. Petersburg) estimated the timing of the biodegradation of matrices from the PLG, PLC and PCL with a thickness of 5; 10 and 15 microns by binding in vitro to the ocular surface of labor

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Ginnan, Roman, Benjamin J. Guikema, Harold A. Singer та David Jourd'heuil. "PKC-δ mediates activation of ERK1/2 and induction of iNOS by IL-1β in vascular smooth muscle cells". American Journal of Physiology-Cell Physiology 290, № 6 (2006): C1583—C1591. http://dx.doi.org/10.1152/ajpcell.00390.2005.

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Although the inflammatory cytokine interleukin-1β (IL-β) is an important regulator of gene expression in vascular smooth muscle (VSM), the signal transduction pathways leading to transcriptional activation upon IL-1β stimulation are poorly understood. Recent studies have implicated IL-1β-mediated ERK1/2 activation in the upregulation of type II nitric oxide synthase (iNOS) in VSM. We report that these events are mediated in a phospholipase C (PLC)- and protein kinase C (PKC)-δ-dependent manner utilizing a signaling mechanism independent of p21ras (Ras) and Raf1 activation. Stimulation of rat a

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Satoh, Akihiko, Anna S. Gukovskaya, Jose M. Nieto та ін. "PKC-δ and -ε regulate NF-κB activation induced by cholecystokinin and TNF-α in pancreatic acinar cells". American Journal of Physiology-Gastrointestinal and Liver Physiology 287, № 3 (2004): G582—G591. http://dx.doi.org/10.1152/ajpgi.00087.2004.

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Although NF-κB plays an important role in pancreatitis, mechanisms underlying its activation remain unclear. We investigated the signaling pathways mediating NF-κB activation in pancreatic acinar cells induced by high-dose cholecystokinin-8 (CCK-8), which causes pancreatitis in rodent models, and TNF-α, which contributes to inflammatory responses of pancreatitis, especially the role of PKC isoforms. We determined subcellular distribution and kinase activities of PKC isoforms and NF-κB activation in dispersed rat pancreatic acini. We applied isoform-specific, cell-permeable peptide inhibitors t

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Moraru, I. I., Mioara Manciulea, Anca Cǎlugǎru, Gr Ghyka, and L. M. Popescu. "Anti-phospholipase C antibodies inhibit the lectin-induced proliferation of human lymphocytes." Bioscience Reports 7, no. 9 (1987): 731–36. http://dx.doi.org/10.1007/bf01116866.

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A novel approach was used to assess the role of phosphoinositide hydrolysis in the mitogenic action of phytohemagglutinin (PHA) or concanavalin A (ConA). The treatment of human peripheral blood leukocytes (PBL) with monospecific antibodies against phospholipase C (PLC) produced a dose-dependent inhibition (up to 100%) of PHA (10 μg/ml) or ConA (25 μg/ml) proliferative effects. Thus, the activation of membrane-bound PLC is a sine-qua-non condition for lectin-induced proliferation of T lymphocytes. The key-role of PLC versus protein kinase C (PKC) is stressed by the fact that the inhibition of P

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Xu, Xuehua, and Tian Jin. "The Novel Functions of the PLC/PKC/PKD Signaling Axis in G Protein-Coupled Receptor-Mediated Chemotaxis of Neutrophils." Journal of Immunology Research 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/817604.

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Chemotaxis, a directional cell migration guided by extracellular chemoattractant gradients, plays an essential role in the recruitment of neutrophils to sites of inflammation. Chemotaxis is mediated by the G protein-coupled receptor (GPCR) signaling pathway. Extracellular stimuli trigger activation of the PLC/PKC/PKD signaling axis, which controls several signaling pathways. Here, we concentrate on the novel functions of PLC/PKC/PKD signaling in GPCR-mediated chemotaxis of neutrophils.

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Irwan, Purnama. "Aplikasi PLC Menggunakan Mikrokontroler PIC 16F877A." Jurnal Sains dan Ilmu Terapan 1, no. 1 (2018): 11–15. http://dx.doi.org/10.59061/jsit.v1i1.112.

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In this study, the use of the PLC application with the PIC 16F877A microcontroller is described, starting from circuit design, assembly, and its use in several applications in PLCs including: setting railroad crossings, distributing goods on conveyors, and setting traffic lights. Programming the ladder diagram of the application is done with the LDmicro program. From the results of this study it is hoped that a PLC device that is more economical, has high reliability and is easy to operate can be made.

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Cheng, Kai-Chun, Ying-Xiao Li, Akihiro Asakawa та ін. "Characterization of preptin-induced insulin secretion in pancreatic β-cells". Journal of Endocrinology 215, № 1 (2012): 43–49. http://dx.doi.org/10.1530/joe-12-0176.

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We aimed to characterize the effects of preptin on insulin secretion at the single-cell level, as well as the mechanisms underlying these changes, with respect to regulation by intracellular Ca2+[Ca2+]imobilization. This study assessed the effect of preptin on insulin secretion and investigated the link between preptin and the phospholipase C (PLC)/protein kinase C (PKC) pathway at the cellular level using fura-2 pentakis(acetoxymethyl) ester-loaded insulin-producing cells (Min 6 cells). Our results demonstrate that preptin promotes insulin secretion in a concentration-dependent manner. Using

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Karpovich, V. V., S. V. Churashov, and V. F. Chernysh. "Comparative experimental study of transparency, mechanical properties and biodegradation of synthetic polymer matrices as possible carriers for cultured limbal epithelial stem cells." Modern technologies in ophtalmology, no. 2 (May 29, 2023): 24–29. http://dx.doi.org/10.25276/2312-4911-2023-2-24-29.

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Purpose. To investigate the transparency, mechanical properties and terms of biodegradation of certain types of synthetic polyester matrices – poly(lactide-glycolide) (PLG), poly(lactide-caprolactone) (PLC) and poly(ε-caprolactone) (PCL) as possible carriers for cultured limbal epithelial stem cells. Material and methods. In the course of the study, the transparency of all polyester matrices of various thicknesses was evaluated by the width of the scattering angle of a helium-neon laser beam with a wavelength of 632.8 nm. The mechanical properties of the matrices (only 5 microns thick) and amn

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Nugroho, Ari, and Yohanes Galih Adhiyoga. "Sistem Otomasi Precision Air Conditioning (PAC) Berbasis PLC Zelio untuk Optimalisasi Energi di Ruang Server." Electrician : Jurnal Rekayasa dan Teknologi Elektro 18, no. 3 (2024): 336–41. https://doi.org/10.23960/elc.v18n3.2724.

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Intisari — Precision Air Conditioning (PAC) dirancang untuk mengontrol suhu dan kelembapan secara presisi di lingkungan kritis seperti ruang server dan laboratorium. Berbeda dari AC konvensional, PAC memfokuskan pada kebutuhan perangkat elektronik sensitif. Penelitian ini mendesain dan mengevaluasi sistem otomatis PAC menggunakan PLC Zelio, dengan metode eksperimen yang meliputi perancangan, pembuatan, pengujian, dan analisis alat. Hasil menunjukkan peningkatan efisiensi energi dan daya tahan mesin, dengan konsumsi daya listrik turun dari 79.1 kW sebelum otomasi menjadi 41.76 kW otomasi pertam

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Stemkowski, Patrick L., Frederick W. Tse, Vera Peuckmann, Christopher P. Ford, William F. Colmers, and Peter A. Smith. "ATP-Inhibition of M Current in Frog Sympathetic Neurons Involves Phospholipase C But Not Ins P3, Ca2+, PKC, orRas." Journal of Neurophysiology 88, no. 1 (2002): 277–88. http://dx.doi.org/10.1152/jn.2002.88.1.277.

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Suppression of the voltage-activated, noninactivating K+ conductance (M conductance; g M) by muscarinic agonists, P2Y agonists or bradykinin increases neuronal excitability. All agonist effects are mediated, at least in part, via the Gq/11 class of G protein. We found, using whole cell or perforated patch recording from bullfrog sympathetic B neurons that ATP-induced suppression of gM was attenuated by the phospholipase C (PLC) inhibitor, U73122 (IC50 ∼0.14 μM) but not by the inactive isomer, U73343 . The ability of extracellularly applied U73122 to inhibit PLC was confirmed by its antagonism

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Zhou, Huiping, and Karnam S. Murthy. "Distinctive G protein-dependent signaling in smooth muscle by sphingosine 1-phosphate receptors S1P1and S1P2." American Journal of Physiology-Cell Physiology 286, no. 5 (2004): C1130—C1138. http://dx.doi.org/10.1152/ajpcell.00429.2003.

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We examined expression of sphingosine 1-phosphate (S1P) receptors and sphingosine kinase (SPK) in gastric smooth muscle cells and characterized signaling pathways mediating S1P-induced 20-kDa myosin light chain (MLC20) phosphorylation and contraction. RT-PCR demonstrated expression of SPK1 and SPK2 and S1P1and S1P2receptors. S1P activated Gq, G13, and all Giisoforms and stimulated PLC-β1, PLC-β3, and Rho kinase activities. PLC-β activity was partially inhibited by pertussis toxin (PTX), Gβ or Gαqantibody, PLC-β1 or PLC-β3 antibody, and by expression of Gαqor Gαiminigene, and was abolished by a

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Tajima, Takuya, Nami Yamaguchi, Yudai Morita, Takuji Yokoe, and Etsuo Chosa. "Novel classification for multi-ligament knee injury including both tibiofemoral joint factor and patellofemoral joint factor (193)." Orthopaedic Journal of Sports Medicine 9, no. 10_suppl5 (2021): 2325967121S0030. http://dx.doi.org/10.1177/2325967121s00304.

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Objectives: Multi-ligament knee injury (MLKI) shows very varied symptoms which was depended on the combination of injured ligaments. Schenck`s knee dislocation classification which was one of useful classifications for surgeon in decision making. However, Schenck`s classification is only referred to the factors of cruciate ligament and collateral ligament. It is well known that knee joint consists of two important structure; tibiofemoral joint and patellofemoral joint. Knee extensor structure is one of important factors of knee function. Dislocation of patella, quadriceps or patella tendon rup

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Chen, Ching-Chow, Jia-Kae Wang та Shwu-Bin Lin. "Antisense Oligonucleotides Targeting Protein Kinase C-α, -βI, or -δ But Not -η Inhibit Lipopolysaccharide-Induced Nitric Oxide Synthase Expression in RAW 264.7 Macrophages: Involvement of a Nuclear Factor κB-Dependent Mechanism". Journal of Immunology 161, № 11 (1998): 6206–14. http://dx.doi.org/10.4049/jimmunol.161.11.6206.

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Abstract The signaling pathway for protein kinase C (PKC) activation and the role of PKC isoforms in LPS-induced nitric oxide (NO) release were studied in RAW 264.7 macrophages. The tyrosine kinase inhibitor genestein attenuated LPS-induced NO release and inducible nitric oxide synthase (iNOS) expression, as did the phosphoinositide-specific phospholipase C (PI-PLC) inhibitor U73122 and the phosphatidylcholine-specific phospholipase C (PC-PLC) inhibitor D609. LPS stimulated phosphatidylinositol (PI) hydrolysis and PKC activity in RAW cells; both were inhibited by genestein. The PKC inhibitors

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Westermann, Robert W., Robert G. Marx, Kurt P. Spindler, et al. "No Difference Between Posterolateral Corner Repair and Reconstruction With Concurrent ACL Surgery: Results From a Prospective Multicenter Cohort." Orthopaedic Journal of Sports Medicine 7, no. 7 (2019): 232596711986106. http://dx.doi.org/10.1177/2325967119861062.

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Background: Injuries to the posterolateral corner (PLC) may occur concurrently with anterior cruciate ligament (ACL) injury. Purpose/Hypothesis: This study evaluated the outcomes of patients who underwent operative management of PLC injuries concurrently with ACL reconstruction in a prospective multicenter cohort. We hypothesized that there would be no differences in outcomes between patients who were treated with PLC repair and PLC reconstruction. Study Design: Cohort study; Level of evidence, 3. Methods: Patients undergoing ACL reconstruction were enrolled into a prospective longitudinal mul

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Hashimoto, Ari, Hidetaka Okada, Aimin Jiang та ін. "Involvement of Guanosine Triphosphatases and Phospholipase C-γ2 in Extracellular Signal–regulated Kinase, c-Jun NH2-terminal Kinase, and p38 Mitogen-activated Protein Kinase Activation by the B Cell Antigen Receptor". Journal of Experimental Medicine 188, № 7 (1998): 1287–95. http://dx.doi.org/10.1084/jem.188.7.1287.

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Mitogen-activated protein (MAP) kinase family members, including extracellular signal–regulated kinase (ERK), c-Jun NH2-terminal kinase ( JNK), and p38 MAP kinase, have been implicated in coupling the B cell antigen receptor (BCR) to transcriptional responses. However, the mechanisms that lead to the activation of these MAP kinase family members have been poorly elucidated. Here we demonstrate that the BCR-induced ERK activation is reduced by loss of Grb2 or expression of a dominant-negative form of Ras, RasN17, whereas this response is not affected by loss of Shc. The inhibition of the ERK re

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Barnett, R. L., L. Ruffini, L. Ramsammy, R. Pasmantier, M. M. Friedlaender, and E. P. Nord. "cGMP antagonizes angiotensin-mediated phosphatidylcholine hydrolysis and C kinase activation in mesangial cells." American Journal of Physiology-Cell Physiology 268, no. 2 (1995): C376—C381. http://dx.doi.org/10.1152/ajpcell.1995.268.2.c376.

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Previous studies from this laboratory have shown that in cultured rat mesangial cells (MC), angiotensin II (ANG II) mediates its effects via activation of phosphatidylinositol-specific phospholipase C (PI-PLC) and phosphatidylcholine-specific phospholipase C (PC-PLC) and phospholipase D (PC-PLD). In addition, guanosine 3',5'-cyclic monophosphate (cGMP)-elevating maneuvers that stimulate particulate and soluble guanylate cyclase [atrial natriuretic factor (ANF) and sodium nitroprusside (SNP), respectively] antagonize ANG II-mediated PI-PLC activation. The current study explored whether cGMP imp

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Banday, Anees Ahmad, та Mustafa F. Lokhandwala. "Oxidative stress reduces renal dopamine D1 receptor-Gq/11α G protein-phospholipase C signaling involving G protein-coupled receptor kinase 2". American Journal of Physiology-Renal Physiology 293, № 1 (2007): F306—F315. http://dx.doi.org/10.1152/ajprenal.00108.2007.

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The dopamine D1 receptors (D1R), expressed in renal proximal tubules, participate in the regulation of sodium transport. A defect in the coupling of the D1R to its G protein/effector complex in renal tubules has been reported in various conditions associated with oxidative stress. Because G protein-coupled receptor kinases (GRKs) are known to play an important role in D1R desensitization, we tested the hypothesis that increased oxidative stress in obese Zucker rats may cause GRK2 upregulation and, subsequently, D1R dysfunction. Lean and obese rats were given normal diet or diet supplemented wi

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Gomes, Pedro, and P. Soares-da-Silva. "Role of cAMP-PKA-PLC signaling cascade on dopamine-induced PKC-mediated inhibition of renal Na+-K+-ATPase activity." American Journal of Physiology-Renal Physiology 282, no. 6 (2002): F1084—F1096. http://dx.doi.org/10.1152/ajprenal.00318.2001.

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We studied the molecular events set into motion by stimulation of D1-like receptors downstream of Na+-K+-ATPase, while measuring apical-to-basal ouabain-sensitive, amphotericin B-induced increases in short-circuit current in opossum kidney (OK) cells. The D1-like receptor agonist SKF-38393 decreased Na+-K+-ATPase activity (IC50, 130 nM). This effect was prevented by the D1-like receptor antagonist SKF-83566, overnight cholera toxin treatment, the protein kinase A (PKA) antagonist H-89, or the PKC antagonist chelerythrine, but not the mitogen-activated PK inhibitor PD-098059 or phosphatidylinos

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Heo, Jung Sun, Yun Jung Lee, and Ho Jae Han. "EGF stimulates proliferation of mouse embryonic stem cells: involvement of Ca2+ influx and p44/42 MAPKs." American Journal of Physiology-Cell Physiology 290, no. 1 (2006): C123—C133. http://dx.doi.org/10.1152/ajpcell.00142.2005.

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We examined the effect of EGF on the proliferation of mouse embryonic stem (ES) cells and their related signal pathways. EGF increased [3H]thymidine and 5-bromo-2′-deoxyuridine incorporation in a time- and dose-dependent manner. EGF stimulated the phosphorylation of EGF receptor (EGFR). Inhibition of EGFR tyrosine kinase with AG-1478 or herbimycin A, inhibition of PLC with neomycin or U-73122, inhibition of PKC with bisindolylmaleimide I or staurosporine, and inhibition of L-type Ca2+ channels with nifedipine or methoxyverapamil prevented EGF-induced [3H]thymidine incorporation. PKC-α, -βI, -γ

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Monick, Martha M., Aaron Brent Carter, Gunnar Gudmundsson, Rama Mallampalli, Linda S. Powers, and Gary W. Hunninghake. "A Phosphatidylcholine-Specific Phospholipase C Regulates Activation of p42/44 Mitogen-Activated Protein Kinases in Lipopolysaccharide-Stimulated Human Alveolar Macrophages." Journal of Immunology 162, no. 5 (1999): 3005–12. http://dx.doi.org/10.4049/jimmunol.162.5.3005.

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Abstract This study uses human alveolar macrophages to determine whether activation of a phosphatidylcholine (PC)-specific phospholipase C (PC-PLC) is linked to activation of the p42/44 (ERK) kinases by LPS. LPS-induced ERK kinase activation was inhibited by tricyclodecan-9-yl xanthogenate (D609), a relatively specific inhibitor of PC-PLC. LPS also increased amounts of diacylglycerol (DAG), and this increase in DAG was inhibited by D609. LPS induction of DAG was, at least in part, derived from PC hydrolysis. Ceramide was also increased in LPS-treated alveolar macrophages, and this increase in

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