Relevant bibliographies by topics / PLEX

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'PLEX'

Author: Grafiati
Published: 4 June 2021
Last updated: 2 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'PLEX.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "PLEX"

1

Hammet, Fleur, Khalid Mahmood, Thomas R. Green, et al. "Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening." BioTechniques 67, no. 3 (2019): 118–22. http://dx.doi.org/10.2144/btn-2019-0026.

Full text
Abstract:
We have previously reported Hi-Plex, a multiplex PCR methodology for building targeted DNA sequencing libraries that offers a low-cost protocol compatible with high-throughput processing. Here, we detail an improved protocol, Hi-Plex2, that more effectively enables the robust construction of small-to-medium panel-size libraries while maintaining low cost, simplicity and accuracy benefits of the Hi-Plex platform. Hi-Plex2 was applied to three panels, comprising 291, 740 and 1193 amplicons, targeting genes associated with risk for breast and/or colon cancer. We show substantial reduction of off-target amplification to enable library construction for small-to-medium-sized design panels not possible using the previous Hi-Plex chemistry.
APA, Harvard, Vancouver, ISO, and other styles
2

Subramanyam, Meena, Tatiana Plavina, Bhupendra O. Khatri, Robert J. Fox, and Susan E. Goelz. "The effect of plasma exchange on serum anti-JC virus antibodies." Multiple Sclerosis Journal 19, no. 7 (2012): 912–19. http://dx.doi.org/10.1177/1352458512467502.

Full text
Abstract:
Objective: Natalizumab, a highly effective treatment for multiple sclerosis (MS) and Crohn’s disease, is associated with progressive multifocal leukoencephalopathy (PML). Upon suspicion or diagnosis of PML, plasma exchange (PLEX) is performed to remove natalizumab from the circulation, allowing immune reconstitution of the central nervous system. Since PLEX may also remove other circulating antibodies, we examined the effects of PLEX on serum immunoglobulin (IgG) and anti–JC virus (JCV) antibody levels in MS patients with and without PML. Methods: Serum samples from 12 natalizumab-treated patients without PML collected before, during and after PLEX were tested for IgG isotypes using a commercial assay, and for anti-JCV antibodies using a two-step enzyme-linked immunosorbent assay. Five natalizumab-treated PML patients who underwent PLEX were also tested for anti-JCV antibodies. Results: PLEX produced a two- to three-fold reduction in all IgG isotypes. Among patients without PML, 42% (five of 12 patients) had detectable anti-JCV antibodies before PLEX; in these patients, anti-JCV antibodies were reduced approximately two- to five-fold, with levels returning to 50–100 percent of baseline two weeks after the final PLEX. The five PML patients, all of whom had detectable anti-JCV antibodies before PLEX, experienced similar reductions in anti-JCV antibody levels following PLEX. Conclusions: Our results indicate that PLEX effectively removes circulating antibodies; however, levels of endogenous anti-JCV antibody, unlike exogenously administered natalizumab, were replenished relatively quickly following PLEX. While interpretation of anti-JCV antibody levels during or within two weeks after PLEX may be problematic, humoral JCV immunity is not abolished by PLEX and antibody levels are rapidly restored.
APA, Harvard, Vancouver, ISO, and other styles
3

Malapati, Sindhu, Sunny R. K. Singh, Rohit Kumar, Jason Mouabbi, and Tarik Hadid. "Plasma Exchange in Thrombotic Microangiopathy: Is It Time Sensitive?" Blood 134, Supplement_1 (2019): 2353. http://dx.doi.org/10.1182/blood-2019-129375.

Full text
Abstract:
Background: Thrombotic microangiopathy (TMA) has myriad causes with only subtle differences at presentation. Thrombotic thrombocytopenic purpura is one of the etiologies, and traditional teaching is that initiation of treatment with plasmapheresis (PLEX) is time sensitive allowing mitigation of the otherwise high morbidity and mortality of this condition. If TTP is suspected, PLEX is frequently initiated prior to availability of confirmatory lab tests. In contradiction to this, a recent publication by Liu et al (Blood 2017) found that early initiation of PLEX was not associated with improved inpatient mortality. We aim to study inpatient outcomes and predictors of early initiation of PLEX in this population. Methods: This is a retrospective cohort analysis of NIS database (year 2016). Our cohort of interest was adult patients with TMA who underwent plasmapheresis and we identified them by selecting those ≥18 years of age, with ICD-10-CM diagnosis code of TMA and procedure code for PLEX. Early PLEX was defined as initiation of PLEX within 24 hrs of admission and late PLEX as beyond 24 hrs. Primary outcome was inpatient mortality and secondary outcomes were length of stay (LOS), predictors of early initiation of PLEX and mean total charge (TOTCHG). Associated factors were analyzed using a multivariate regression model. We used STATA for statistical analysis. Results: A total of 2064 admissions with thrombotic microangiopathies (TMA) were identified in 2016, of which 1200 (58.1%) received PLEX. Death in the same admission occurred in 120 (5.8%) of all TMA patients, but only in 45 (3.75%) of those who underwent PLEX. Among those who received PLEX for TMA, mean age was 49.2 years, 71.25% (n=855) had early PLEX, 70.4% were female, 38.75% Caucasian and 38.3% African American. The mean time to initiation of PLEX was 2.01 days. Within the cohort of those who received PLEX, inpatient mortality was lower in those with early initiation of PLEX (OR 0.13, p 0.005) and higher with increasing age (OR 1.10, p 0.006) after adjusting for gender and all hospital characteristics (geographical location, urban or rural location of hospital, hospital teaching status, size). LOS was shorter in early PLEX vs late PLEX (by 5.25 days, p 0.0001) and was longer in those admitted to a teaching hospital vs non teaching hospital (by 3.08 days, p 0.038) keeping income quartile of patient's address, gender, Charlson Comorbidity Index (CCI) and other hospital characteristics constant. Likelihood of receiving PLEX within 24 hours of admission was lower in those who were admitted over the weekend (OR 0.36, p 0.006) after adjusting for other factors. There was no association of early initiation of PLEX with age, gender, race, CCI, mean income quartile for patient's address, insurance status, hospital region, urban or rural location of hospital, hospital teaching status. Total charge was approximately $54,759 higher (with a trend to significance, p=0.054) for those receiving late PLEX versus early PLEX after adjusting for patient demographics and hospital characteristics. Conclusion: Among patients admitted with TMA, inpatient mortality, length of stay and mean total charge for hospitalization was lower in those who had initiation of plasmapheresis within 24 hours of admission. Admissions over weekends were associated with lower likelihood of early initiation of PLEX. Given the better inpatient outcomes with early initiation of PLEX, this should be an area of quality improvement interventions in the future. Table Disclosures No relevant conflicts of interest to declare.
APA, Harvard, Vancouver, ISO, and other styles
4

Valente, Guido, Giuseppina Nicotra, Marisa Arrondini, et al. "Co-Expression of Plexin-B1 and Met in Human Breast and Ovary Tumours Enhances the Risk of Progression." Analytical Cellular Pathology 31, no. 6 (2009): 423–36. http://dx.doi.org/10.1155/2009/151392.

Full text
Abstract:
Background: Plex-B1, the receptor of Sema4D, has been implicated in tumour growth, angiogenesis and metastasis. The binding of Sema4D to Plex-B1 can trigger the activation of Met tyrosine kinase, thereby promoting cell dissociation and invasive growth. We tested the hypothesis that the expression of Plex-B1, either alone or in association with Met, can be of predictive value for tumour progression.Methods: The expression and distribution of Plex-B1 and Met were investigated by immunohistochemistry and immunofluorescence in 50 human neoplasias originating in the breast and ovary, and correlated with clinical–pathological data at diagnosis.Results: Plex-B1 and Met were individually expressed in 14% and in 24% of the tumours, respectively. Plex-B1 and Met were co-expressed in 24/50 cases (48%), and in the majority of these (83%) Met was tyrosine phosphorylated. The expression of Plex-B1 or Met alone showed no significant correlation with tumour aggressiveness, whereas advanced stage tumours (III–IV) frequently showed Plex-B1–Met double-positive (9/13). Tumours co-expressing Plex-B1 and Met were characterised by worse grading and higher incidence of lymph node metastases. Out of 22 tumours with lymph node metastases, as many as 19 were Plex-B1 and Met double-positive (p=0.0008), and 17 expressed phosphorylated Met (p=0.002).Conclusions: Plex-B1 assumes a predictive value for unfavourable outcome when co-expressed with Met.
APA, Harvard, Vancouver, ISO, and other styles
5

Bonnan, Mickael, Rudy Valentino, Stéphane Debeugny, et al. "Short delay to initiate plasma exchange is the strongest predictor of outcome in severe attacks of NMO spectrum disorders." Journal of Neurology, Neurosurgery & Psychiatry 89, no. 4 (2017): 346–51. http://dx.doi.org/10.1136/jnnp-2017-316286.

Full text
Abstract:
IntroductionSevere attacks of neuromyelitis optica spectrum disorder (NMO-SD) are improved by plasma exchange (PLEX) given as an adjunctive therapy. Initial studies failed to demonstrate a delay of PLEX treatment influenced clinical outcome; however PLEX was always used late. We examine the clinical consequences of delay in PLEX initiation on severe optic neuritis and spinal cord attacks in NMO-SD.MethodsAll of our patients who suffered attacks of NMO-SD, treated in our centre by PLEX, were retrospectively considered for inclusion. Primary outcome was defined as complete improvement. Secondary poor/good outcomes were respectively defined to be the higher/lower third of Delta-Expanded Disability Status Scale (EDSS) (late minus baseline EDSS). Delays from clinical onset to PLEX initiation were categorised for multivariate analysis.ResultsOf the 60 patients included, NMO-SD criteria (2015) were fulfilled in 92%. One hundred and fifteen attacks were included and received PLEX with a median of 7 days (0–54) after clinical onset. The probability to regain complete improvement continuously decreased from 50% for PLEX given at day 0 to 1%–5% after day 20. Through multivariate analysis, the baseline impairment and PLEX delay were associated with the probability to complete improvement (OR 5.3; 95% CI 1.8 to 15.9). Reducing the PLEX delay also influenced the good secondary outcome but not the poor secondary outcome.ConclusionsThese results confirm an improved clinical benefit of early initiation of PLEX during severe attacks of NMO-SD. Perceiving PLEX as a rescue therapy only after steroid failure could be deleterious.
APA, Harvard, Vancouver, ISO, and other styles
6

Bonnan, Mickael, and Philippe Cabre. "Plasma Exchange in Severe Attacks of Neuromyelitis Optica." Multiple Sclerosis International 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/787630.

Full text
Abstract:
Background. Neuromyelitis optica (NMO) attacks are poorly controlled by steroids and evolve in stepwise neurological impairments. Assuming the strong humoral response underlying NMO attacks, plasma exchange (PLEX) is an appropriate technique in severe NMO attacks.Objective. Presenting an up-to-date review of the literature of PLEX in NMO.Methods. We summarize the rationale of PLEX in relation with the physiology of NMO, the main technical aspects, and the available studies.Results. PLEX in severe attacks from myelitis or optic neuritis are associated with a better outcome, depending on PLEX delay (“time is cord and eyes”). NMO-IgG status has no influence. Finally, we build up an original concept linking the inner dynamic of the lesion, the timing of PLEX onset and the expected clinical results.Conclusion. PLEX is a safe and efficient add-on therapy in NMO, in synergy with steroids. Large therapeutic trials are required to definitely assess the procedure and define the time opportunity window.
APA, Harvard, Vancouver, ISO, and other styles
7

Vennegoor, A., T. Rispens, BW Van Oosten, et al. "Application of serum natalizumab levels during plasma exchange in MS patients with progressive multifocal leukoencephalopathy." Multiple Sclerosis Journal 21, no. 4 (2014): 481–84. http://dx.doi.org/10.1177/1352458514541507.

Full text
Abstract:
Progressive multifocal leukoencephalopathy (PML) is a severe complication of natalizumab treatment. Restoring immune function by plasmapheresis/immunoadsorption (PLEX/IA) is important for the outcome of PML. We report on four multiple sclerosis (MS) patients whom developed PML during natalizumab treatment, in whom we measured serum natalizumab concentrations before and during PLEX. Depending on the serum natalizumab concentration at the time of PML diagnosis, the number of PLEX treatments necessary to reach subtherapeutic serum natalizumab concentrations is variable. Measuring serum natalizumab concentrations before and during PLEX is helpful to determine the optimum number of PLEX treatments in individual MS patients with PML.
APA, Harvard, Vancouver, ISO, and other styles
8

Skorupka, Nic, Andrei Miclea, Katarzyna Aleksandra Jalowiec, et al. "Visual Outcomes of Plasma Exchange Treatment of Steroid-Refractory Optic Neuritis: A Retrospective Monocentric Analysis." Transfusion Medicine and Hemotherapy 46, no. 6 (2019): 417–22. http://dx.doi.org/10.1159/000504027.

Full text
Abstract:
Introduction: In acute inflammatory optic neuritis (ON) as a typical onset of multiple sclerosis (MS), only few studies have investigated plasma exchange (PLEX) as a sequential treatment after insufficient response to high-dose intravenous glucocorticosteroids. Therefore, we aimed to investigate treatment outcome on visual acuity (VA) with PLEX in patients with steroid-refractory ON. Methods: In our retrospective monocentric study, medical records were screened for patients with acute ON as their first relapse with sequential MS diagnosis or with an established MS diagnosis from the Bern University Hospital (Switzerland) that were treated with PLEX between 2016 and 2018 due to lacking steroid response. VA prior to steroid administration, and before and after PLEX were assessed and compared using the Friedman multiple comparison test. Results: In total, 18 patients were included in the analysis. Interval from symptom onset to PLEX was 20.3 days (mean, 95% CI 14.8–25.9). Relevant functional improvement (VA of ≥0.5, after a mean of 15.9 (13.3–18.5) days after start of PLEX) was detected in 16/18 (88.9%) with a significant amelioration as compared to VA before glucocorticosteroids and before PLEX (p < 0.0001). VA improvement at a later time point (38.1 weeks, 25.2–51.0) was present in 15/16 (93.8%) patients. No serious adverse events were detected. PLEX could be performed via peripheral access in 13/18 patients (72.2%). Conclusion: Our study demonstrates significant improvements of VA with PLEX in a cohort of MS patients with steroid-refractory ON. High response rates may be due to the timely treatment initiation. Despite the small sample size, our data support the early use of PLEX in steroid-refractory ON with a favorable safety profile.
APA, Harvard, Vancouver, ISO, and other styles
9

Bomback, Andrew S., and Gerald B. Appel. "ANCA-associated GN—to PLEX or not to PLEX?" Nature Reviews Nephrology 9, no. 8 (2013): 436–38. http://dx.doi.org/10.1038/nrneph.2013.126.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Komalasari, Ema, Winiati P. Rahayu, and Siti Nurjanah. "Evaluation of multiplex SYBR green real-time PCR assay for detection of pathogenic Escherichia coli." International Journal of Engineering & Technology 9, no. 2 (2020): 448. http://dx.doi.org/10.14419/ijet.v9i2.30389.

Full text
Abstract:
Pathogenic Escherichia coli (E. coli) has been implicated in a wide range of disease causing infections. It is essential to generate a method for detecting and differentiating each pathotype of E. coli which is more quickly and efficiently by using less reagent. This study aimed to evaluate a SYBR Green multiplex real-time PCR method for detecting four types of pathogenic E. coli. Two of multiplex real-time PCR system, 6-plex and 3-plex, were set to detect six different virulence factors from ETEC, EPEC, EHEC, and EIEC and evaluate the melting curves and specificity compared to simplex method. The results showed that 3-plex rt-PCR method gave more reliable melting curves than 6-plex. The 3-plex rt-PCR also provided similar melting value (Tm) to simplex system. The results of this specificity assay supported the selection of 3-plex rt-PCR conditions for detection of pathogenic E. coli.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "PLEX"

1

Sheridan, Jon-Phillip. "Plex." VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/2491.

Full text
Abstract:
This thesis explores the evolution of my practice as it developed over my two years of graduate school. I entered school interested in the built environment and how structures helped create subjectivity and provided sites for material and phenomenological transformations. Early in graduate school, I developed a photography series that investigated these issues. However, an awareness of a conversation occurring in the larger art world that questioned the efficacy of photography drove me to consider ways to extend my practice into sculpture and installation. Over the next two semesters, I developed my ideas of light, form and structure into video and sculpture installations that extended these ideas out into the space of the viewer. Ultimately, an interest in light and surface led me back to photography and to the realization that a studio practice that focused on photography about photography opened up avenues that allowed a photographic practice not to become stagnant but rather to grow and expand.
APA, Harvard, Vancouver, ISO, and other styles
2

Möller, Johan. "Exekveringsmiljö för Plex-C på JVM." Thesis, Linköping University, Department of Computer and Information Science, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1688.

Full text
Abstract:
<p>The Ericsson AXE-based systems are programmed using an internally developed language called Plex-C. Plex-C is normally compiled to execute on an Ericsson internal processor architecture. A transition to standard processors is currently in progress. This makes it interesting to examine if Plex-C can be compiled to execute on the JVM, which would make it processor independent. </p><p>The purpose of the thesis is to examine if parts of the run-time environment of Plex-C can be translated to Java and if this can be done so that sufficient performance is obtained. It includes how language constructions in Plex-C can be translated to Java. </p><p>The thesis describes how a limited part of the Plex-C run-time environment is implemented in Java. Optimizations are an important part of the implementation. </p><p>It is also described how the JVM system was tested with a benchmark test. </p><p>The test results indicate that the implemented system is a few times faster than the Ericsson internal processor architecture. But this performance is still not sufficient for the JVM system to be an interesting replacement for the currently used processor architecture. It might still be useful as a processor independent test platform.</p>
APA, Harvard, Vancouver, ISO, and other styles
3

Lindhult, Johan. "Operational Semantics for PLEX : A Basis for Safe Parallelization." Licentiate thesis, Västerås : School of Innovation, Design and Engineering, Mälardalen University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-631.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Narmack, Samuel. "Functionalization and Evaluation of Nanoparticle Probes for the Development of a 14-Plex Diagnostic assay." Thesis, KTH, Kemi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-299949.

Full text
Abstract:
Detta projekt var ett samarbete mellan Aplex Bio AB och Scilifelab. Projektets mål var att utveckla en molekylär diagnostisk panel med förmågan att detektera och diskriminera mellan 14 olika typer av patogener. Projektet innehåller 4 kapitel med fokus på olika mål. I första kapitlet utvecklades en metod för att karakterisera emissioner av fluorescerande nanopartikel kluster. Den första utvärderade metoden utnyttjade klick-kemi för att binda nanopartiklarna till makrostrukturer uppbyggda av amplifierat DNA. Den andra utvärderade metoden skapade aggregerade komplex av nanopartiklar med amplifierat DNA för att utvärdera partiklarnas emissioner. I kapitel 2 av projektet användes azid-funktionaliserade nanopartiklar levererade av Aplex Bio AB för att tvärbinda DBCO modifierade oligonukleotider. Sedan utvecklades en hybridiserings baserad metod för att kvantifiera relativa mängden oligonukleotider på partiklarna. Denna metod användes för att reproducerbart funktionalisera partiklar och utveckla nanopartikel-sonder som kan binda till DNA genom hybridisering. I kapitel 2 utvärderades även hur effektivt och specifikt de utvecklade nanopartikel-sonderna hybridiserar till DNA. I kapitel 3 utvärderades amplifiering av syntetiska ssDNA sekvenser valda från genetiska markörer av 14 patogener, DNA amplifierades med metoden RCA. Målet var att utvärdera specificiteten av amplifieringen. Specifik amplifiering av varje DNA sekvens i panelen var en förutsättning för att möjliggöra detektion och diskriminering av alla patogener i panelen. I kapitel 4 var målet att utveckla en kostnadseffektiv metod för att funktionalisera nanopartiklar med oligonukleotid sekvenser. För att göra detta användes DBCO-NHS-ester reagens och amin-modifierade oligonukleotider. Förverkligande av detta projekt skulle skapa en diagnostisk panel med potentialen att påverka det diagnostiska fältet på en global skala. När detta projekt är fullt utvecklat kan panelen modifieras för detektion av önskade DNA/RNA sekvenser vilket möjliggör ett mångfalt av applikationer, detta skulle göra panelen konkurrerande med dagens diagnostiska metoder då den kan användas i existerande mikroskopiuppsättningar.<br>This work was a collaboration between Aplex Bio AB and Scilifelab with the aim of developing a molecular assay capable of detecting and discriminating between 14 different pathogenic targets. There are 4 chapters with focus on different goals. In chapter one a method of evaluating emissions of fluorescent nanoparticle clusters was developed. The first approach of evaluating nanoparticle emissions was to utilize click chemistry to bind nanoparticles to macroscale structures of amplified DNA targets. The second evaluated approach was the formation of aggregated complexes of nanoparticles and amplified DNA targets. The second chapter of the thesis used azide functionalized nanoparticles supplied by Aplex Bio AB to utilize azide groups as crosslinkers and use them to functionalize the nanoparticles with DBCO oligos. A hybridization-based method was then developed to quantify relative oligo densities on the nanoparticles, enabling reproducible oligo functionalization of nanoparticles, producing nanoparticle probes that can bind to DNA. The final task of chapter 2 was evaluating the binding efficiency and specificity of the developed nanoparticle probes. The third chapter of the thesis evaluated amplification of synthetic ssDNA sequences corresponding to genetic markers of 14 pathogenic targets using RCA. The goal was to confirm specificity of chosen padlock probes and corresponding synthetic targets for each pathogen. Specific amplification of each target was a prerequisite to enable detecting and discriminating between the 14 pathogenic targets. In chapter 4 the goal was to develop a cost-effective method of oligo functionalization for nanoparticles. This chapter evaluated two main approaches of using DBCO-NHS-ester reagents to perform DBCO modification of amine-oligos. The realization of this work would develop an assay that has the potential to impact the field of diagnostics on a global scale. When fully developed, the molecular assay can be modified to detect any RNA/DNA targets which enables numerous applications, making the assay a competitive diagnostic tool which can be implemented in existing microscopy systems.
APA, Harvard, Vancouver, ISO, and other styles
5

Trouchet, Amandine. "PCR digitale pour la détection et la caractérisation de micro-organismes pathogènes au niveau de la cellule unique." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC170/document.

Full text
Abstract:
Nous avons pour but de développer un système microfluidique en gouttes, capable, à l’échelle de la cellule/bactérie unique, de détecter et de co-localiser plusieurs marqueurs génétiques, en utilisant une version digitale et multiplexée de la réaction de polymérisation en chaîne (PCR). Les systèmes de PCR digitale actuellement commercialisés ne le permettent toujours pas. Un tel prototype garantira la présence de multiples marqueurs à l’intérieur d’un même génome, ce qui permettra l’identification du pathogène avec précision et un taux de faux-positifs proche de zéro. Comme première application, nous démontrerons la possibilité de co-localiser quatre gènes de virulence de la souche O157:H7 d’Escherichia coli, un pathogène majeur, qui est détecté dans des échantillons alimentaires ou provenant de fèces cliniques pouvant aussi contenir des E. coli non pathogènes porteuses d’une partie des gènes de virulence. Avant de procéder à des tests TaqMan multicolores en point final, E. coli sera d’abord encapsulée dans des gouttes micrométriques et lysée par la chaleur in situ. Notre objectif est de démontrer que ce test peut être appliqué avec succès à un petit ensemble d’échantillons cliniques ou alimentaires<br>We aim to develop a prototype of droplet-based microfluidic system capable of detecting and colocalizing multiple genetic markers at the single cell/bacteria level, using the Polymerase Chain Reaction (PCR) in a digital multiplexed version. This cannot be achieved using current commercial digital PCR systems, and should increase the sensitivity and reliability of the detection of pathogens. Importantly, the system will guarantee the presence of multiple markers within the same genome and enable accurate identification, and bring the false positive rate close to zero. As a first application, we will demonstrate the possibility to co-localize 3 virulence genes in the E.coli strain O157:H7, a major foodborne pathogen, which has to be detected in clinical feces samples or food samples, which may also contain non pathogenic E. coli carrying only a subset of these virulence genes. E. Coli will be encapsulated in micrometric droplets, lysed by heating in situ prior performing a multicolour end-point Taqman assay. Our objective is to demonstrate that this test can be successfully applied to real clinical or food samples
APA, Harvard, Vancouver, ISO, and other styles
6

Trukhanov, Svyatoslav. "Novel approaches for solving large-scale optimization problems on graphs." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2986.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Sazima, Ricardo. "PLEX MPLS : analise, projeto e implementação de uma plataforma para experimentos com MPLS com suporte a QoS." [s.n.], 2004. http://repositorio.unicamp.br/jspui/handle/REPOSIP/259128.

Full text
Abstract:
Orientador: Mauricio Ferreira Magalhães<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica e de Computação<br>Made available in DSpace on 2018-08-04T17:32:51Z (GMT). No. of bitstreams: 1 Sazima_Ricardo_M.pdf: 1107563 bytes, checksum: a1179beaec699c0dcd87b45284e4a99c (MD5) Previous issue date: 2004<br>Resumo: Dados a banda de transmissão atualmente disponível, o protocolo (IP) utilizado para transmitir a maior parte de tráfego e a quantidade de tráfego e seus requisitos de aos, a Engenharia de Tráfego (TE, de Traffic Engineering) tomou-se um componente cada vez mais importante nas redes de comunicações. O padrão do IETF para enncaminhamento roteamento entitulado Multi-Protocol Label Switching (MPLS) preenche lacunas importantes neste cenário e é peça chave das metodologias de TE mais sofisticadas. Nesta dissertação é apresentado um modelo genérico de TE e uma proposta para uma Plataforma para Experimentos com MPLS (PLEX MPLS) que permite que o usuário defina, execute, e analise configurações de Engenharia de Tráfego com MPLS em uma rede IP. Os principais objetivos da PLEX MPLS são: . Estudo da tecnologia MPLS e suporte a outros trabalhos em andamento no contexto do grupo de estudos em MPLS do DCA . Experimentos com engenharia de tráfego com MPLS: validação das tecnologias e conceitos relacionados . Experimentos didáticos em disciplinas de laboratório de redes. Os principais conceitos de TE e MPLS são apresentados como referência, bem como uma descrição do NIST Switch, a plataforma MPLS escolhida para este trabalho. A análise, projeto e implementação da PLEX MPLS são apresentados, pois formam parte significativa do trabalho desenvolvido. A PLEX não só utiliza, como também estende as funcionalidades oferecidas pelo NIST Switch a fim de oferecer um esquema de TE mais completo e eficiente. Na fase de análise, vemos quais os principais requisitos para a implementação desta plataforma, seguindo uma metodologia de Engenharia de Software. Na fase de projeto, vemos as soluções propostas para os problemas identificados na fase de análise e temos uma especificação dos componentes a serem implementados. Na fase seguinte, discutimos a implementação das principais características dos componentes da PLEX, justificando as decisões tomadas. Para validar a implementação da PLEX de acordo com sua proposta, foram realizados alguns experimentos em uma rede de testes com tráfego real. A execução destes experimentos é descrita e seus resultados analisados. Os resultados obtidos assinalam claramente a importância e utilidade de esquemas de TE baseada em MPLS. Uma interessante metodologia para TE, compilada a partir de várias propostas, é apresentada. Finalmente, apontam-se caminhos a seguir em um trabalho futuro de refinamento da PLEX<br>Abstract: Given the bandwidth currently available, the protocol (IP) used to transmit most Internet traffic, the quantity of traffic produced and its QoS requirements, Traffic Engineering (TE) has become an increasingly important component of communications networks. IETF's standard for forwarding/routing, which is entitled Multi-Protocol Label Switching (MPLS), presents important solutions in this scenario playing a major role in more sophisticated TE methodologies. This work presents a generic methodology for TE and a proposal for a Platform for Experiments with MPLS (PLEX MPLS) which allows the user to define, execute and analyze Traffic Engineering configurations with MPLS in an IP network. The main goals of PLEX MPLS are: . Study of the M PLS technology and support of other ongoing works with M PLS in the DCA . Traffic Engineering experiments with MPLS: validation of the related concepts and technologies . Support of didactic experiments in academic disciplines. The main concepts of MPLS and TE are presented as reference, as well as a brief description of NIST Switch, the MPLS software chosen for the PLEX MPLS implementation. The analysis, project and implementation of PLEX MPLS are presented, since are significant part of the developed work. PLEX not only uses, but also extends NIST Switch functionalities to offer a more complete and efficient TE scheme. In the analysis phase the main requirements for the PLEX implementation are specified, following a well-known Software Engineering methodology. The solutions found for the problems identified in the analysis phase are presented in the project phase alongside with a specification of the components that will be implemented. In the next phase, the implementation of PLEX is discussed focusing on the most important characteristics of PLEX components and justifying the implementation ecisions. In order to validate PLEX implementation and its proposal, some experiments were made in a test network with reallive traffic. These experiments are described and its results analyzed. The results obtained clearly indicate the importance and utility of TE schemes based on MPLS. Also an interesting TE methodology compiled from several proposals is presented. Finally, possible improvements and future work on PLEX MPLS are indicated.<br>Mestrado<br>Engenharia de Computação<br>Mestre em Engenharia Elétrica
APA, Harvard, Vancouver, ISO, and other styles
8

Basten, Mirka [Verfasser]. "mRNA-basierte Quantifizierung von Biomarkern zur Diagnostik von zervikalen intraepithelialen Neoplasien und von Zervixkarzinomen : Validierung des QuantiGene® 2.0 Plex Assays / Mirka Basten." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2019. http://d-nb.info/1189138999/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Smårs, Jonathan. "Implementing Digital Fun : Locating success factors in PC games." Thesis, Karlstads universitet, Handelshögskolan, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-39160.

Full text
Abstract:
The purpose of this paper is to explore the technical implementation of common game design theory in successful PC games today. The study uses a quantitative study to analyze 23 modern successful PC games to identify common success factors which are connected to Arrasvuors et al. (2009) theory of the Playful Experiences Framework, Sutton-Smiths (2001) seven rhetorics of play and Max-Neefs (1991) human needs matrix. The results is a practical checklist of 63 success factors for use in game development. These success factors are present in the successful games and described for implementation in game design for the PC platform. These success factors are then divided into the 7 categories: freedom, immersion, challenge, multiplayer, personal, preference and human needs to provide a better overview of the success factor checklist and connect them to proven game design theory.
APA, Harvard, Vancouver, ISO, and other styles
10

Cha, Wonhee. "Application of multiplex branched DNA method for the detection and study of avian inlfuenza [i.e. influenza] virus." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1211475165.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "PLEX"

1

Zuravicky, Orli. Fun with Plex. Simon Spotlight, 2009.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Zuravicky, Orli. Fun with Plex. Simon Spotlight, 2009.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Zuravicky, Orli. Fun with Plex. Simon Spotlight, 2009.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

ill, Zdanowicz Paul, ed. Play with Plex. Simon Spotlight, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Riva, Richard de La. Les " PLEX", une tradition renouvelée. Société canadienne d'hypothèques et de logement, 1997.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Riva, Richard de La. Plex housing: A renewed tradition. CMHC, 2000.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

In the plex: How Google thinks, works, and shapes our lives. Simon & Schuster, 2011.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Weigend, Thomas. Absprachen in ausländischen Strafverfahren: Eine rechtsvergleichende Untersuchung zu konsensualen Elementen im Strafprozess. Max-Planck-Institut für Ausländisches und Internationales Strafrecht, 1990.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Beernaert, Marie-Aude. Repentis et collaborateurs de justice dans le système pénal: Analyse comparée et critique. Bruylant, 2002.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Plen. A. Shneer, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "PLEX"

1

Okubo, Yoshiaki, Masanobu Matsudaira, and Makoto Haraguchi. "Detecting Maximum k-Plex with Iterative Proper ℓ-Plex Search." In Discovery Science. Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-11812-3_21.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Hamilton, Robert G. "Single-Plex Immunoassays and Autoanalyzers." In Encyclopedia of Medical Immunology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-9194-1_300.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Wright, Susan J. "PLEX - A Structured Line Diagram Editor." In Automating Systems Development. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1033-4_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Bunke, H., and B. Haller. "A parser for context free plex grammars." In Graph-Theoretic Concepts in Computer Science. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/3-540-52292-1_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Kozarewa, Iwanka, and Daniel J. Turner. "96-Plex Molecular Barcoding for the Illumina Genome Analyzer." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-089-8_20.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Bunke, Horst, and Bernhard Haller. "Syntactic Analysis of Context—Free Plex Languages for Pattern Recognition." In Structured Document Image Analysis. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77281-8_24.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Okubo, Yoshiaki, Makoto Haraguchi, and Etsuji Tomita. "Structural Change Pattern Mining Based on Constrained Maximal k-Plex Search." In Discovery Science. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-33492-4_23.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Karlin-Neumann, George, Bin Zhang, and Claudia Litterst. "Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_24.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

van Asch, Barbara, and António Amorim. "Capillary Electrophoresis Analysis of a 9-plex STR Assay for Canine Genotyping." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-461-2_16.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Phillips, C., M. Fondevila, and Maria Victoria Lareau. "A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry Investigations." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-461-2_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "PLEX"

1

Yoon, Sang Ho, Ke Huo, and Karthik Ramani. "Plex." In UbiComp '14: The 2014 ACM Conference on Ubiquitous Computing. ACM, 2014. http://dx.doi.org/10.1145/2638728.2638746.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Lucero, Andrés, and Juha Arrasvuori. "PLEX Cards." In the 3rd International Conference. ACM Press, 2010. http://dx.doi.org/10.1145/1823818.1823821.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Morton, Richard D., Imelda C. Stambaugh, Gregg S. Weaver, Michael K. Ewert, and Kathryn M. Hurlbert. "BIO-Plex Thermal Control System Design." In 31st International Conference On Environmental Systems. SAE International, 2001. http://dx.doi.org/10.4271/2001-01-2324.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Jones, Harry W. "BIO-Plex Operations Planning and Scheduling." In International Conference On Environmental Systems. SAE International, 1998. http://dx.doi.org/10.4271/981750.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Overland, David. "Advanced Control System Architecture for BIO-Plex." In International Conference On Environmental Systems. SAE International, 2003. http://dx.doi.org/10.4271/2003-01-2576.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Theodoracatos, Vassilios E., and Xiaogang Guan. "Computer-Aided Design Synthesis Using Syntactic Solid Modeling." In ASME 1994 Design Technical Conferences collocated with the ASME 1994 International Computers in Engineering Conference and Exhibition and the ASME 1994 8th Annual Database Symposium. American Society of Mechanical Engineers, 1994. http://dx.doi.org/10.1115/detc1994-0083.

Full text
Abstract:
Abstract This paper presents a new Computer-Aided Design (CAD) synthesis model which uses Plex Grammar as structural relationship descriptors and NURBS surface representation for constructing standard and non-standard solid entities. Here, the designer uses a syntactic design methodology for early topological and geometrical definition of the structure of concept alternatives resulting from the design process. This syntactic scheme provides the capability of describing a large set of complex structures by using a small set of simple entities. The recursive nature of the grammar and the hierarchical representation of the structure makes the description of complex structures simple and under the direct control of the designer. An object structure constructive tree is generated and subsequently translated into Plex Grammar production rules in order to form an Interconnection Matrix (ICM) expressing. The resulting Plex structure defined in the ICM expresses the topological information among entities which form the specific types of objects. By modifying the Plex grammar rules, various objects with different geometry and topology can easily be reconstructed. Compared to conventional solid modeling techniques, this approach provides more systematic object generation, easy manipulation and modification, control over congruity and the ability to represent sculptured shapes. Several examples of syntactic solid modeling applied in design synthesis will be presented for further usage in downstream applications.
APA, Harvard, Vancouver, ISO, and other styles
7

Rodriguez, Luis F., Sukwon Kang, John A. Hogan, and Cory Finn. "Modeling of a Composting System within BIO-Plex." In 31st International Conference On Environmental Systems. SAE International, 2001. http://dx.doi.org/10.4271/2001-01-2323.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Jones, Harry, Cory Finn, Xianmin Kwauk, and Charles Blackwell. "Modeling Separate and Combined Atmospheres in BIO-Plex." In 31st International Conference On Environmental Systems. SAE International, 2001. http://dx.doi.org/10.4271/2001-01-2361.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Garland, J. L., R. Fortson, N. Packham, and J. Sager. "Testing Bioregenerative Waste Processing Approaches in BIO-Plex." In International Conference On Environmental Systems. SAE International, 1999. http://dx.doi.org/10.4271/1999-01-2189.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Arrasvuori, Juha, Marion Boberg, Jussi Holopainen, Hannu Korhonen, Andrés Lucero, and Markus Montola. "Applying the PLEX framework in designing for playfulness." In the 2011 Conference. ACM Press, 2011. http://dx.doi.org/10.1145/2347504.2347531.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "PLEX"

1

Kotongan, Victoria Hazel. 14-plex Feasibility Report. Office of Scientific and Technical Information (OSTI), 2013. http://dx.doi.org/10.2172/1084235.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Latkowski, J. F., and S. Reyes. Plan for PLEX X-Ray Ablation Experiments and Analysis. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/15013365.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Duffin, Matthew L. Plea-Bargaining in International Criminal Tribunals: A Legitimate and Necessary Tool. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada370523.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Peterson, Dominic S., Claudine E. Armenta, and Jung H. Rim. Deliverable for FαST project: Ln Resin based PLE. Office of Scientific and Technical Information (OSTI), 2012. http://dx.doi.org/10.2172/1040014.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Langendorf, Samuel. Magneto-Inertial Fusion and the Plasma Liner Experiment (PLX) [Slides]. Office of Scientific and Technical Information (OSTI), 2021. http://dx.doi.org/10.2172/1810490.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Farr, Kathryn. Negotiating the guilty plea: a study of the process of felony case disposition in one urban court system. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.875.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Stark, Jon. Testing a report. Crossref, 2020. http://dx.doi.org/10.5555/kjafioakljhj098u89.

Full text
Abstract:
Dum mia juneco mi vivis en la komunisma Orienta Eŭropo kaj tie oni instruis, ke estas du mondrigardoj: la religia kaj la scienca darvinisma. Oni instruis, ke la darvinisma koncepto estas materiisma kaj pro tio oni kutime pensas, ke Darwin estis ateisto. Sed tio tute ne estas vera. Li skribis la venontajn frazojn en sia verko „La Origino de Specioj per Natura Selektado, aŭ konservado de la plej vivkapablaj rasoj en la batalo por vivo”:
APA, Harvard, Vancouver, ISO, and other styles
8

van der Geest, Matthijs, Erik Meesters, and Sander Mücher. Impact of terrestrial erosion on coral reef health at Bonaire: a plea for nature-inclusive "watershed-to-reef" based coastal management. Wageningen Marine Research, 2020. http://dx.doi.org/10.18174/524688.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'PLEX' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography