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Journal articles on the topic 'PMN'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2025

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1

Kaslovsky, R. A., L. Gibbs, and A. B. Malik. "Neutrophils from chronic granulomatous disease fail to increase endothelial permeability." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 4 (1991): H1226—H1230. http://dx.doi.org/10.1152/ajpheart.1991.261.4.h1226.

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Studies of neutrophil-dependent endothelial injury were made using normal neutrophils (nl-PMN, i.e., normal polymorphonuclear neutrophils) and PMN obtained from a patient with X-linked chronic granulomatous disease (CGD-PMN). Layering of nl-PMN on bovine pulmonary microvessel endothelial monolayers (ratio 10:1) followed by challenge with phorbol 12-myristate 13-acetate (PMA; 5 x 10(-9) M) resulted in a 190% increase in 125I-labeled albumin permeability across the monolayers. Pretreatment of endothelial monolayers with tumor necrosis factor-alpha (TNF-alpha; 200 or 1,000 U/ml) further enhanced
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2

Chen, X., M. Tzanela, M. K. Baumgartner, J. R. McCormick, and J. D. Catravas. "PMA-activated neutrophils decrease ectoenzyme activities in rabbit aortic endothelial cells in culture." American Journal of Physiology-Lung Cellular and Molecular Physiology 263, no. 6 (1992): L657—L663. http://dx.doi.org/10.1152/ajplung.1992.263.6.l657.

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We have studied the effects of phorbol 12-myristate 13-acetate (PMA)-activated neutrophils [polymorphonuclear leukocytes (PMN)] on endothelial ectoenzyme [angiotensin-converting enzyme (ACE) and 5'-nucleotidase (NCT)] activities in cultured rabbit aortic endothelial cells (EC) with the use of [3H]benzoyl-Phe-Ala-Pro and 14C-labeled AMP as substrates, respectively, under first-order reaction conditions. PMA (1–1,000 ng/ml) or PMN alone had no effect on ACE activity. When PMA was incubated together with PMN (PMN/EC = 1.25:1 or 2.5 x 10(5) neutrophils/ml) for 4 h in Earle's salts, a PMA dose-depe
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3

Luo, Jun, Shujun Zhang, Wesley Hackenberger, and Thomas R. Shrout. "PIN–PMN–PT piezoelectric crystals with increased rhombohedral-to-tetragonal phase transition temperature." Journal of Advanced Dielectrics 04, no. 01 (2014): 1450001. http://dx.doi.org/10.1142/s2010135x14500015.

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In this work, crystal growth and characterization of PIN–PMN–PT (29–59% PIN and 28–35% PT) were conducted to understand how PIN ratio in the PIN–PMN–PT system impacts its phase stability during crystallization. High-quality PIN–PMN–PT crystals with 36% PIN were obtained using the self-seeded Bridgman process, even though the cubic phase In 2 O 3 formed at the very beginning of solidification. The melt became more unstable when the PIN ratio in the PIN–PMN–PT system increased to 49% and above, which affected the composition and quality of the as-grown crystals significantly. By increasing the P
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4

Whitin, J. C., and H. J. Cohen. "Dissociation between aggregation and superoxide production in human granulocytes." Journal of Immunology 134, no. 2 (1985): 1206–11. http://dx.doi.org/10.4049/jimmunol.134.2.1206.

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Abstract Aggregation and the activation of the granulocyte (PMN) superoxide (O2-) generating system occur when certain stimuli are added to resting cells. It had previously been postulated that PMN aggregation is essential for maximal O2- production. This study was undertaken to test the hypothesis that PMN aggregation is required for full expression of PMN O2- production. We examined aggregation and O2- production induced by four stimuli; concanavalin A (Con A), phorbol myristate acetate (PMA), N-formylmethionyl-leucyl-phenylalanine (FMLP), and ionophore A23187. Cytochalasin B enhanced aggreg
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5

GAO, JUNJIE, ZHUO XU, FEI LI, et al. "THE HYDROSTATIC PIEZOELECTRICITY OF RELAXOR-PbTiO3 FERROELECTRIC CERAMICS AND CRYSTALS." Journal of Advanced Dielectrics 02, no. 03 (2012): 1250018. http://dx.doi.org/10.1142/s2010135x1250018x.

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The hydrostatic piezoelectricity of Pb ( Mg 1/3 Nb 2/3)–x PbTiO 3 (PMN–xPT) ceramics and PMN–0.32PT and Pb(Mg1/3Nb2/3)–Pb(In1/2Nb1/2)–PbTiO3 (PMN–PIN–PT) crystals were investigated through a quasi-static method. The results showed that pressure-induced depolarization occurred in PMN–xPT ceramics when pressure was above about 50 MPa, while there was no obvious pressure-induced depolarization for PMN–0.32PT and PMN–PIN–PT crystals in the whole pressure range (0~530 MPa). The hydrostatic piezoelectric coefficient d h of PMN–0.32PT and PMN–PIN–PT crystals was nearly independent of pressure. The d
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6

Nath, J., Y. Ohno, J. I. Gallin, and D. G. Wright. "A novel post-translational incorporation of tyrosine into multiple proteins in activated human neutrophils. Correlation with phagocytosis and activation of the NADPH oxidase-mediated respiratory burst." Journal of Immunology 149, no. 10 (1992): 3360–71. http://dx.doi.org/10.4049/jimmunol.149.10.3360.

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Abstract Activation of human neutrophils by PMA causes a post-translational incorporation of 14C-labeled tyrosine into multiple neutrophil (PMN) proteins, that is distinctly different from the enzymatic tyrosinolation of tubulin in FMLP-stimulated PMN. Post-translational incorporation of other radiolabeled amino acids, including the structurally similar amino acid phenylalanine, does not occur under identical conditions of neutrophil activation, suggesting an involvement of the phenolic hydroxyl group of tyrosine in the PMA-mediated reaction. Similar to the stimulation of PMN tubulin tyrosinol
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7

Symington, F. W. "CDw17: a neutrophil glycolipid antigen regulated by activation." Journal of Immunology 142, no. 8 (1989): 2784–90. http://dx.doi.org/10.4049/jimmunol.142.8.2784.

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Abstract The plasma membrane and intracellular granules of human polymorphonuclear neutrophils (PMN) contain large amounts of the glycolipid, lactosylceramide (LacCer; Gal beta 1----4Glc beta 1----1Cer). Despite its abundance, novel subcellular distribution, and lineage-restricted expression, nothing of PMN LacCer function is known. We examined the relationship between LacCer and PMN activation by assessing binding of anti-LacCer mAb (T5A7; anti-CDw17) to PMN during and after cell stimulation. CDw17 expression markedly decreased after treatment with PMA, dioctanoylglycerol, calcium ionophore,
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8

Zhang, Weiguang, Jijun Li, Yongming Xing, et al. "Determination of the Mechanical Properties of PIN–PMN–PT Bulk Single Crystals by Nanoindentation." Crystals 10, no. 1 (2020): 28. http://dx.doi.org/10.3390/cryst10010028.

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The present study aimed to experimentally evaluate the mechanical properties of Pb(In1/2Nb1/2)O3–Pb(Mg1/3Nb2/3)O3–PbTiO3 (PIN–PMN–PT) bulk single crystals with different crystallographic directions using the nanoindentation technique. The load–indentation depth curves, elastic and plastic deformations, hardnesses, and Young’s moduli of [100]- and [110]-oriented 0.28PIN–0.43PMN–0.29PT bulk single crystals were investigated. Our results show that with an increase in the maximum indentation depth hmax, the plastic residual percentage increased for both the [100]- and the [110]-oriented single cry
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9

VAN OOSTVELDT, KAAT, CHRISTIAN BURVENICH, FERNANDO MOREIRA DA SILVA, and ANNE-MARIE MASSART-LEËN. "Respiratory burst activity in activated and unstimulated isolated bovine blood neutrophils during experimentally induced Escherichia coli mastitis." Journal of Dairy Research 66, no. 3 (1999): 375–83. http://dx.doi.org/10.1017/s0022029999003623.

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The respiratory burst activity, measured as H2O2 production, of isolated bovine polymorphonuclear leucocytes (PMN) was evaluated during experimentally induced Escherichia coli mastitis by means of flow cytometry in cells activated by phorbol 12-myristate 13-acetate (PMA) and in unstimulated cells. As expected, a significantly reduced respiratory burst activity was observed in PMA-activated PMN 18 h after intramammary inoculation with Escherichia coli. At this time only 75% of the PMA-activated PMN showed a respiratory burst, but with a higher intensity than that measured before and later after
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10

LEE, Ho-Yong. "“Generation III” Piezoelectric Single Crystals Developed by Solid-State Single Crystal Growth Method." Ceramist 24, no. 3 (2021): 273–85. http://dx.doi.org/10.31613/ceramist.2021.24.3.07.

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Crystallographically engineered Relaxor-PT single crystals, specifically PMN-PT (Generation I) and PIN-PMN-PT/PMN-PZT (Generation II), offer much higher piezoelectric and electromechanical coupling coefficients (d33>1,500 pC/N, k33>0.9), when compared to polycrystalline PZT-5H ceramics (d33>600 pC/N, k33>0.75). Recently Ceracomp Co., Ltd. (www.ceracomp.com) has developed the solid-state single crystal growth (SSCG) technique and successfully fabricated Gen III PMN-PZT single crystals modified with acceptors or donors. The piezoelectric constants (d33) of (001) Gen III PMN-PZT singl
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11

Kilpatrick, J. M., and J. E. Volanakis. "Opsonic properties of C-reactive protein. Stimulation by phorbol myristate acetate enables human neutrophils to phagocytize C-reactive protein-coated cells." Journal of Immunology 134, no. 5 (1985): 3364–70. http://dx.doi.org/10.4049/jimmunol.134.5.3364.

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Abstract We examined phagocytosis of sheep erythrocytes passively sensitized with pneumococcal C-polysaccharide (E-PnC) and of E-PnC coated with C-reactive protein (E-PnC-CRP) by human polymorphonuclear leukocytes (PMN). PMN isolated from blood of normal individuals failed to ingest either E-PnC or E-PnC-CRP; however, after stimulation with 12-O-tetradecanoylphorbol-13-acetate (PMA; 2 ng/ml), PMN ingested E-PnC-CRP efficiently with a mean phagocytic index (PI) of 99.5 +/- 4.8 (mean +/- SD, n = 11), and E-PnC to a lesser extent with a mean PI of 33.2 +/- 11.7 (mean +/- SD, n = 11). PMN that had
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12

Tian, Jian, and Pengdi Han. "Crystal growth and property characterization for PIN–PMN–PT ternary piezoelectric crystals." Journal of Advanced Dielectrics 04, no. 01 (2014): 1350027. http://dx.doi.org/10.1142/s2010135x13500276.

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Binary piezoelectric crystal Pb ( Mg 1/3 Nb 2/3) O 3– PbTiO 3 (PMN–PT) has excellent dielectric and piezoelectric properties, which has led to the commercialization for medical ultrasound imaging. Recently, there is a growing demand for piezoelectric crystals with improved thermal and electrical properties. Ternary piezoelectric crystals Pb ( In 1/2 Nb 1/2) O 3– Pb ( Mg 1/3 Nb 2/3) O 3– PbTiO 3 (PIN–PMN–PT) have increased depoling temperature and coercive field than binary PMN–PT. To better understand the ternary crystal system and improve crystal properties, we systematically investigated cry
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13

Kaslovsky, R. A., L. Lai, K. Parker, and A. B. Malik. "Mediation of endothelial injury following neutrophil adherence to extracellular matrix." American Journal of Physiology-Lung Cellular and Molecular Physiology 264, no. 4 (1993): L401—L405. http://dx.doi.org/10.1152/ajplung.1993.264.4.l401.

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Since polymorphonuclear leukocytes (PMN) rapidly migrate across the endothelial barrier and attach to extracellular matrix components, we tested the hypothesis that adhesion of PMN to matrix proteins can mediate endothelial injury following PMN activation. Studies were made using gelatin- and fibronectin-coated polycarbonate microporous filters (10 microns thick) on which confluent monolayers of bovine pulmonary microvessel endothelial cells were grown. PMN were layered either directly onto endothelial cells (at a ratio of 10:1) (“upright system”) or onto gelatin- and fibronectin-coated filter
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14

Zhang, Qiaozhen, Rufan Du, Baichuan Li, et al. "Piezoelectric acoustic wave characteristics of Pb(In0.5Nb0.5)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 single crystal substrate: A comparative study with and without SiO2 overlay." Journal of Applied Physics 132, no. 2 (2022): 024103. http://dx.doi.org/10.1063/5.0095058.

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This paper investigates the excitation and propagation characteristics of a piezoelectric acoustic wave propagating in a rotated Y-cut X-propagating Pb(In0.5Nb0.5)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 (PIN-PMN-PT) relaxor-based ferroelectric single crystal substrate. Numerical simulations were performed to evaluate the acoustic wave properties by FEM software COMSOL. For comparison, two types of structures are taken into consideration: one is the traditional metal electrode/YX-PIN-PMN-PT and the other is silicon dioxide (SiO2) overlay/metal electrode/YX-PIN-PMN-PT substrate. It is shown that shear-horizo
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15

Bender, J. G., D. E. Van Epps, and D. E. Chenoweth. "Independent regulation of human neutrophil chemotactic receptors after activation." Journal of Immunology 139, no. 9 (1987): 3028–33. http://dx.doi.org/10.4049/jimmunol.139.9.3028.

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Abstract The fluoresceinated chemotactic factors, C5a, formyl-methionyl-leucyl-phenylalanyl-lysine (FMLPL), and casein were used in conjunction with flow cytometry to examine chemotactic factor receptor expression on polymorphonuclear leukocytes (PMN) activated with phorbol myristate acetate (PMA), C5a, or formyl-methionyl-leucyl-phenylalanine. Activation with PMA resulted in a dose-dependent increase in binding of fluorescein-labeled (FL)-casein and (FL-FMLPL) over the range of PMA concentrations from 0.5 to 50 ng/ml. In contrast, activation of PMN with PMA resulted in a dose-dependent decrea
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16

Amer, Johnny, and Eitan Fibach. "Chronic Oxidative Stress Reduces the Oxidative Burst Response of Thalassemic Polymorphonuclear Neutrophils." Blood 104, no. 11 (2004): 3811. http://dx.doi.org/10.1182/blood.v104.11.3811.3811.

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Abstract Bacterial infections are a serious complication in the beta-thalassemia syndrome, but the mechanisms underlying the increased susceptibility to these infections are not fully understood. Factors, likely to be involved are anemia, splenectomy, iron-overload, alterations in immune responses and defective phagocytic cells. Polymorphonuclear neutrophils (PMN) destroy microbial organisms by a burst of reactive oxygen species (ROS) in response to bacterial components as well as to phorbol myristate acetate (PMA). The respiratory burst is mediated by a multi-component enzyme - NADPH oxidase.
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17

Nauseef, William M. "Pin-ing down PMN priming." Blood 116, no. 26 (2010): 5788–89. http://dx.doi.org/10.1182/blood-2010-11-315846.

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18

Kovacs, Iren B., R. H. Meyrick Thomas, A. R. Mackay, M. H. A. Rustin, and J. D. T. Kirby. "Increased chemiluminescence of polymorphonuclear leucocytes from patients with progressive systemic sclerosis." Clinical Science 70, no. 3 (1986): 257–61. http://dx.doi.org/10.1042/cs0700257.

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1. Chemiluminescence (CL) of whole blood and isolated polymorphonuclear leucocytes (PMN) from patients with systemic sclerosis (SS) and from age and sex matched controls was measured. CL was induced by the addition of particles (zymosan, latex beads), or phorbol myristate acetate (PMA) or chemotactic peptide (FMLP). 2. Whole blood CL (induced by PMA, zymosan or latex particles) was significantly greater in SS patients than in normal controls. 3. Isolated PMN CL (induced by PMA, FMLP or latex particles) was also significantly greater in the SS patients compared with controls. 4. Increased CL or
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19

Caimi, G., B. Canino, F. Ferrara, M. Montana, and R. Lo Presti. "Leucocyte Rheology at Baseline and after Activation in Post-Phlebitic Syndrome." Phlebology: The Journal of Venous Disease 14, no. 3 (1999): 100–104. http://dx.doi.org/10.1177/026835559901400303.

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Objective: To evaluate leucocyte rheology, expressed as leucocyte filtration, polymorphonuclear leucocyte (PMN) membrane fluidity and cytosolic Ca2+ concentration in subjects with post-phlebitic leg syndrome (PPS). Methods: In 22 subjects with PPS we determined leucocyte filtration [unfractionated, mononuclear (MN) and PMN cells], employing the St George Filtrometer, PMN membrane fluidity using the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) and PMN cytosolic Ca2+ concentration using the fluorescent probe Fura 2-AM. Subsequently we determined the same PMN
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20

Doerschuk, C. M., R. K. Winn, H. O. Coxson, and J. M. Harlan. "CD18-dependent and -independent mechanisms of neutrophil emigration in the pulmonary and systemic microcirculation of rabbits." Journal of Immunology 144, no. 6 (1990): 2327–33. http://dx.doi.org/10.4049/jimmunol.144.6.2327.

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Abstract Neutrophil (PMN) migration in the systemic and pulmonary circulation of rabbits was compared by using different inflammatory stimuli to determine the role of the leukocyte adhesion complex, CD11/CD18, in each of these vascular beds. The adhesion complex was blocked by administering the anti-CD18 mAb 60.3. The data show that mAb 60.3 blocks PMN emigration into inflammatory foci in the abdominal wall produced by implanting sponges containing either hydrochloric acid, Streptococcus pneumoniae, Escherichia coli endotoxin, or PMA. mAb 60.3 also inhibited PMN emigration in response to perit
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21

Sun, Xinlei, Fujie Shi, Weiran Wang, et al. "Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury." Biomedicines 10, no. 5 (2022): 1193. http://dx.doi.org/10.3390/biomedicines10051193.

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Infiltration of polymorphonuclear neutrophils (PMNs) plays a central role in acute lung injury (ALI). The mechanisms governing PMN inflammatory responses, however, remain incompletely understood. Based on our recent study showing a non-metabolic role of pyruvate kinase type M2 (PKM2) in controlling PMN degranulation of secondary and tertiary granules and consequent chemotaxis, here we tested a hypothesis that Pkm2-deficient mice may resist ALI due to impaired PMN inflammatory responses. We found that PMN aerobic glycolysis controlled the degranulation of secondary and tertiary granules induced
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22

Zhao, Wei, Zengzhe Xi, Pinyang Fang, et al. "Electric and Optical Properties of Pb(Er1/2Nb1/2)-Pb(Mg1/3Nb1/3)-PbTiO3 Crystals." Crystals 8, no. 11 (2018): 426. http://dx.doi.org/10.3390/cryst8110426.

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New ferroelectric crystals Pb(Er1/2Nb1/2)-Pb(Mg1/3Nb1/3)O-PbTiO3 (PEN-PMN-PT) were grown by using the flux method. Phase structure of the crystals was described by the X-ray diffraction analysis. Dielectric, ferroelectric and optical properties of the PEN-PMN-PT crystals were investigated systematically. Higher Curie temperature (Tc ~ 291 °C) and larger coercive field (Ec ~ 17.6 kV/cm) for the 40PEN-13PMN-47PT can be obtained, respectively, compared with those of the PMN-PT. Moreover, strong green and red emissions can be excited by using the 980 nm laser. The PEN-PMN-PT crystals with these pe
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23

Meng, J. F., Z.-Y. Cheng, Brajesh K. Rai, et al. "Photoluminescence in PbMg1/3Nb2/3O3–PbIn1/2Nb1/2O3 systems." Journal of Materials Research 13, no. 7 (1998): 1861–64. http://dx.doi.org/10.1557/jmr.1998.0264.

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Photoluminescence for PbMg1/3Nb2/3O3 (PMN)– PbIn1/2Nb1/2O3 (PIN) solid solutions in the temperature ranging from 35 to 295 K have been obtained for the first time. An abrupt photoluminescence enhancement of the system has been observed to occur at ∼210 K, which can be attributed to the growing and merging of dynamic polar microregions or to the phase transformation of the material at this temperature. The PIN content has been found to affect the photoluminescence of the PMN-PIN system significantly. The photoluminescence mechanism for PMN-PIN has been studied.
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24

Perry, M. L., S. G. Kayes, J. W. Barnard, and A. E. Taylor. "Effects of phorbol myristate acetate-stimulated human leukocytes on rat lung." Journal of Applied Physiology 68, no. 1 (1990): 235–40. http://dx.doi.org/10.1152/jappl.1990.68.1.235.

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Human blood was separated into polymorphonuclear (PMN) and mononuclear (MN) leukocyte fractions, and 3 x 10(7) cells (PMN or MN) were added to isolated rat lungs perfused with 5% human albumin in buffer and stimulated with phorbol myristate acetate (PMA). Lungs perfused with either albumin alone, PMN, or MN but not stimulated with PMA showed no change in vascular resistance or endothelial permeability measured as the capillary filtration coefficient (Kf,c). Lungs that were stimulated with PMA with no cells showed no change in Kf,c (0.34 +/- 0.07 vs. 0.37 +/- 0.7), but vascular resistance incre
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25

Caldwell, S. E., L. F. Cassidy, and J. S. Abramson. "Alterations in cell protein phosphorylation in human neutrophils exposed to influenza A virus. A possible mechanism for depressed cellular end-stage functions." Journal of Immunology 140, no. 10 (1988): 3560–67. http://dx.doi.org/10.4049/jimmunol.140.10.3560.

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Abstract When polymorphonuclear leukocytes (PMN) are exposed to most harvests of influenza A virus (depressing virus, DV) for 20 min, chemotactic, secretory, and oxidative functions are depressed upon subsequent exposure to soluble or particulate stimuli. Other harvests of influenza A virus (non-DV) do not alter these activities. The DV-induced changes in multiple functions suggest the virus may interfere with steps involved in PMN activation. Because some of these steps may be regulated by protein phosphorylation, we examined the effect of non-DV and DV on cellular protein phosphorylation. PM
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26

MEHRZAD, JALIL, HILDE DOSOGNE, EVELYNE MEYER, ROGER HEYNEMAN, and CHRISTIAN BURVENICH. "Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation." Journal of Dairy Research 68, no. 3 (2001): 399–415. http://dx.doi.org/10.1017/s0022029901005039.

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The non-stimulated and phorbol 12-myristate 13-acetate (PMA)-stimulated luminol-augmented cellular chemiluminescence (CL) response and viability of milk and blood polymorphonuclear leukocytes (PMN) were determined in lactating dairy cows during different stages of lactation. In the first study, ten healthy cows each in early, mid and late lactation were compared. In a second study, the same measurements as in the first study were evaluated longitudinally in 12 cows during 1 month following parturition. The CL activity and myeloperoxidase (MPO) content of milk PMN and macrophages (M) were also
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27

Mócsai, Attila, Erzsébet Ligeti, Clifford A. Lowell, and Giorgio Berton. "Adhesion-Dependent Degranulation of Neutrophils Requires the Src Family Kinases Fgr and Hck." Journal of Immunology 162, no. 2 (1999): 1120–26. http://dx.doi.org/10.4049/jimmunol.162.2.1120.

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Abstract Polymorphonuclear neutrophils (PMN) adherent to integrin ligands respond to inflammatory mediators by reorganizing their cytoskeleton and releasing reactive oxygen intermediates. As Src family tyrosine kinases are implicated in these responses, we investigated their possible role in regulating degranulation. Human PMN incubated on fibrinogen released lactoferrin in response to TNF-α and this response was inhibited by PP1, a Src family tyrosine kinase inhibitor. This drug had no effect on lactoferrin secretion induced by PMA, an adhesion-independent agonist of PMN degranulation. Howeve
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28

Chen, X., and J. D. Catravas. "Neutrophil-mediated endothelial angiotensin-converting enzyme dysfunction: role of oxygen-derived free radicals." American Journal of Physiology-Lung Cellular and Molecular Physiology 265, no. 3 (1993): L243—L249. http://dx.doi.org/10.1152/ajplung.1993.265.3.l243.

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We examined the mechanisms whereby phorbol 12-myristate 13-acetate (PMA)-activated rabbit peritoneal neutrophils [polymorphonuclear leukocytes (PMN)] altered endothelial-bound angiotensin-converting enzyme (ACE) activity in cultured bovine pulmonary arterial endothelial cells (EC). PMA or PMN alone had no effect on ACE activity. When PMN were coincubated with PMA (10 ng/ml) for 4 h in Earle's salt solution, endothelial ACE activity was decreased by 87%. No EC cytotoxicity was observed at this time as determined by 51Cr release from prelabeled EC. Activated PMN-mediated decreased ACE activity w
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29

King, CH, CA Peck, CS Haimes, et al. "Modulation of human neutrophil effector functions by monoclonal antibodies against surface membrane molecules of 94,000 and 180,000 molecular weight." Blood 67, no. 1 (1986): 188–94. http://dx.doi.org/10.1182/blood.v67.1.188.188.

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Abstract Function-related antigens on the neutrophil (PMN) surface were identified using two newly developed PMN-specific mouse monoclonal antibodies. These IgG antibodies, designated Ab 1–14 and Ab 1–15, were selected for detailed study after initial testing revealed their significant inhibition of PMN superoxide generation in response to N- formyl-Met-Leu-Phe (FMLP) (64% for 1–14 and 64% for 1–15; P less than .05). In further experiments, Ab 1–14 augmented PMN adhesion (by 111%; P less than .01) and degranulation (by 52%; P less than .05) in response to FMLP, while Ab 1–15 inhibited these re
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30

King, CH, CA Peck, CS Haimes, et al. "Modulation of human neutrophil effector functions by monoclonal antibodies against surface membrane molecules of 94,000 and 180,000 molecular weight." Blood 67, no. 1 (1986): 188–94. http://dx.doi.org/10.1182/blood.v67.1.188.bloodjournal671188.

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Function-related antigens on the neutrophil (PMN) surface were identified using two newly developed PMN-specific mouse monoclonal antibodies. These IgG antibodies, designated Ab 1–14 and Ab 1–15, were selected for detailed study after initial testing revealed their significant inhibition of PMN superoxide generation in response to N- formyl-Met-Leu-Phe (FMLP) (64% for 1–14 and 64% for 1–15; P less than .05). In further experiments, Ab 1–14 augmented PMN adhesion (by 111%; P less than .01) and degranulation (by 52%; P less than .05) in response to FMLP, while Ab 1–15 inhibited these responses b
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31

Grabovsky, S., I. Shnaidshtein, J. Przeslawski, Zhenrong Li, Zhuo Xu, and B. Strukov. "Specific heat of a PIN-PMN-PT single-crystal up to 500K." Journal of Advanced Dielectrics 07, no. 05 (2017): 1750032. http://dx.doi.org/10.1142/s2010135x17500321.

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The temperature dependence of the specific heat of the single crystal Pb(In[Formula: see text]Nb[Formula: see text])O3-Pb(Mg[Formula: see text]Nb[Formula: see text])O3-PbTiO3 (PIN-PMN-PT) was obtained at 80–500[Formula: see text]K by means of ac-calorimetry, and comparison with the calorimetric data for PMN was made, allowing one to determine absolute values of the specific heat of PIN-PMN-PT. For the high-temperature region ([Formula: see text][Formula: see text]K), the background heat capacity was calculated, the anomalies of the heat capacity for both compounds were determined, and the hump
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32

Evangelista, V., S. Manarini, S. Rotondo, et al. "Platelet/polymorphonuclear leukocyte interaction in dynamic conditions: evidence of adhesion cascade and cross talk between P-selectin and the beta 2 integrin CD11b/CD18." Blood 88, no. 11 (1996): 4183–94. http://dx.doi.org/10.1182/blood.v88.11.4183.4183.

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Abstract Adhesion between platelets and polymorphonuclear leukocytes (PMN) is a key event in thrombosis and inflammation. Double color fluorescence- activated cell sorter (FACS) analysis was used to determine the extent and kinetics of adhesion of thrombin-activated platelets to resting or activated PMN when mixed cell populations were incubated in dynamic conditions. Activated platelets bound very rapidly to PMN. Mixed cell conjugates reached a maximum at 1 minute and were reversible within 10 minutes. Platelet/PMN adhesion required both Ca2+ and Mg2+ and was markedly increased by the presenc
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33

Evangelista, V., S. Manarini, S. Rotondo, et al. "Platelet/polymorphonuclear leukocyte interaction in dynamic conditions: evidence of adhesion cascade and cross talk between P-selectin and the beta 2 integrin CD11b/CD18." Blood 88, no. 11 (1996): 4183–94. http://dx.doi.org/10.1182/blood.v88.11.4183.bloodjournal88114183.

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Adhesion between platelets and polymorphonuclear leukocytes (PMN) is a key event in thrombosis and inflammation. Double color fluorescence- activated cell sorter (FACS) analysis was used to determine the extent and kinetics of adhesion of thrombin-activated platelets to resting or activated PMN when mixed cell populations were incubated in dynamic conditions. Activated platelets bound very rapidly to PMN. Mixed cell conjugates reached a maximum at 1 minute and were reversible within 10 minutes. Platelet/PMN adhesion required both Ca2+ and Mg2+ and was markedly increased by the presence of Mn2+
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34

Zhang, Yazhao, Xianxin Wu, Wanlin Wang, et al. "Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses." PeerJ 10 (October 14, 2022): e14118. http://dx.doi.org/10.7717/peerj.14118.

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Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (an obligate biotrophic pathogen) is a worldwide threat to wheat production that occurs over a wide geographic area in China. For monitoring genetic variation and virulence structure of Blumeria graminis f. sp. tritici in Liaoning, Heilongjiang, and Sichuan in 2015, 31 wheat lines with known Powdery mildew resistance genes and 2 EST-SSR markers were used to characterize the virulence and genetic diversity. Results indicated that 90% of all isolates were virulent on Pm3c, Pm3e, Pm3f, Pm4a, Pm5, Pm6 (Timgalen), Pm7, Pm16, Pm19, and
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35

Ou, Yun, Yingying Wu, and Jinlin Peng. "Misfit-Strain Phase Diagram, Electromechanical and Electrocaloric Responses in Epitaxial PIN–PMN–PT Thin Films." Materials 15, no. 21 (2022): 7660. http://dx.doi.org/10.3390/ma15217660.

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xPb(In1/2Nb1/2)O3-(1−x−y)Pb(Mg1/3Nb2/3)O3−yPbTiO3 (PIN–PMN–PT) bulks possess excellent electromechanical coupling and dielectric properties, but the corresponding epitaxial PIN–PMN–PT thin films have not yet been explored. This paper adopts a nonlinear thermodynamics analysis to investigate the influences of misfit strains on the phase structures, electromechanical properties, and electrocaloric responses in epitaxial PIN–PMN–PT thin films. The misfit strain–temperature phase diagram was constructed. The results reveal that the PIN–PMN–PT thin films may exist in tetragonal c-, orthorhombic aa-
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36

Wan, Yuhui, Zhenrong Li, Ming Ma, et al. "Dielectric behavior and phase transition in [111]-oriented PIN–PMN–PT single crystals under dc bias." Journal of Advanced Dielectrics 04, no. 01 (2014): 1450004. http://dx.doi.org/10.1142/s2010135x14500040.

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Temperature and electric field dependences of the dielectric behavior and phase transition for [111]-oriented 0.23PIN–0.52PMN–0.25PT (PIN-PMN–0.25PT) and 0.24PIN–0.43PMN–0.33PT (PIN–PMN–0.33PT) single crystals were investigated over a temperature range from -100°C to 250°C using field-heating (FH) dielectric measurements. The transition phenomenon from ferroelectric microdomain to macrodomain was found in rhombohedra (R) phase region in the single crystals under dc bias. This transition temperature Tf of micro-to-macrodomain is sensitive to dc bias and move quickly to lower temperature with in
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37

Sellak, H., E. Franzini, J. Hakim, and C. Pasquier. "Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation." Blood 83, no. 9 (1994): 2669–77. http://dx.doi.org/10.1182/blood.v83.9.2669.2669.

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Abstract We compared the effects of phorbol 12-myristate 13-acetate (PMA) and thrombin with those of nonlytic concentrations of reactive oxygen species (ROS) generated by hypoxanthine (HX)-xanthine oxidase (XO) on the adhesion properties of human umbilical cord vein endothelial cells (HUVEC) to resting polymorphonuclear neutrophils (PMN). PMN adherence to HX-XO-treated HUVEC was increased approximately twofold to 2.5-fold relative to untreated HUVEC, both immediately and after 2 hours. It was not additive to that induced by PMA or thrombin stimulation of HUVEC. ROS-induced adherence was not du
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Sellak, H., E. Franzini, J. Hakim, and C. Pasquier. "Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation." Blood 83, no. 9 (1994): 2669–77. http://dx.doi.org/10.1182/blood.v83.9.2669.bloodjournal8392669.

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We compared the effects of phorbol 12-myristate 13-acetate (PMA) and thrombin with those of nonlytic concentrations of reactive oxygen species (ROS) generated by hypoxanthine (HX)-xanthine oxidase (XO) on the adhesion properties of human umbilical cord vein endothelial cells (HUVEC) to resting polymorphonuclear neutrophils (PMN). PMN adherence to HX-XO-treated HUVEC was increased approximately twofold to 2.5-fold relative to untreated HUVEC, both immediately and after 2 hours. It was not additive to that induced by PMA or thrombin stimulation of HUVEC. ROS-induced adherence was not due to plat
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39

Siflinger-Birnboim, A., D. C. Bode, and A. B. Malik. "Adenosine 3',5'-cyclic monophosphate attenuates neutrophil-mediated increase in endothelial permeability." American Journal of Physiology-Heart and Circulatory Physiology 264, no. 2 (1993): H370—H375. http://dx.doi.org/10.1152/ajpheart.1993.264.2.h370.

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We studied the effects of 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) and cholera toxin (CT) on 125I-labeled albumin flux across confluent monolayers of bovine pulmonary microvessel endothelial cells (BMVEC) grown on polycarbonate filters (10(5) BMVEC/filter). 8-BrcAMP and CT increased endothelial adenosine 3',5'-cyclic monophosphate (cAMP) concentrations about twofold. Polymorphonuclear leukocytes (PMN) were layered on BMVEC monolayers (ratio of 10:1) and activated with phorbol 12-myristate 13 acetate (PMA; 5 x 10(-9) M). Transendothelial 125I-labeled albumin clearance rate was mea
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40

Combadiere, C., J. el Benna, E. Pedruzzi, J. Hakim, and A. Perianin. "Stimulation of the human neutrophil respiratory burst by formyl peptides is primed by a protein kinase inhibitor, staurosporine." Blood 82, no. 9 (1993): 2890–98. http://dx.doi.org/10.1182/blood.v82.9.2890.2890.

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Abstract Stimulation of polymorphonuclear neutrophils (PMN) by phorbol esters or formyl peptides (fMLP) generates large quantities of superoxide anion, the so-called respiratory burst (RB), a phenomenon associated with intense phosphorylation of a 47-kD protein (p47 phox). Staurosporine, a potent protein kinase C (PKC) antagonist, inhibits both responses when PMN are stimulated by phorbol myristate acetate (PMA), suggesting a positive role of PKC. In this study, we reassessed these PMN responses in fMLP-stimulated cells and found that staurosporine had opposite effects depending on the duratio
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41

Combadiere, C., J. el Benna, E. Pedruzzi, J. Hakim, and A. Perianin. "Stimulation of the human neutrophil respiratory burst by formyl peptides is primed by a protein kinase inhibitor, staurosporine." Blood 82, no. 9 (1993): 2890–98. http://dx.doi.org/10.1182/blood.v82.9.2890.bloodjournal8292890.

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Stimulation of polymorphonuclear neutrophils (PMN) by phorbol esters or formyl peptides (fMLP) generates large quantities of superoxide anion, the so-called respiratory burst (RB), a phenomenon associated with intense phosphorylation of a 47-kD protein (p47 phox). Staurosporine, a potent protein kinase C (PKC) antagonist, inhibits both responses when PMN are stimulated by phorbol myristate acetate (PMA), suggesting a positive role of PKC. In this study, we reassessed these PMN responses in fMLP-stimulated cells and found that staurosporine had opposite effects depending on the duration of PMN
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42

Skulski, Ryszard, Paweł Wawrzała, Dariusz Bochenek, Jan Kulawik, Dorota Szwagierczak, and Przemysław Niemiec. "Multilayer Ceramic Capacitors Based on the PMN-PT-PFN Solid Solution." Advances in Science and Technology 77 (September 2012): 41–46. http://dx.doi.org/10.4028/www.scientific.net/ast.77.41.

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We present the technology of obtaining and the main properties of multilayered ceramic capacitors (MLCC) based on the PMN-PT-PFN solid solution. PMN-PT-PFN is the abbreviation of the material with general formula (1-y)[(1-x)Pb(Mg1/3Nb2/3)O3-xPbTiO3]-yPb(Fe1/2Nb1/2)O3. In our work the investigated material was PMN-PT-PFN with x=0.25, y=0.1 i.e. 0.9(0.75PMN-0.25PT)-0.1PFN. The powder of 0.9(0.75PMN-0.25PT)-0.1PFN has been obtained in three steps. In first step we obtained MgNb2O6. In second step FeNbO4 was obtained. In final third step the 0.9(0.75PMN-0.25PT)-0.1PFN was obtained from mixed powde
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43

Kuroishi, Toshinobu, Ken-ichi Komine, Ken-ichi Asai, et al. "Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells." Clinical Diagnostic Laboratory Immunology 10, no. 6 (2003): 1011–18. http://dx.doi.org/10.1128/cdli.10.6.1011-1018.2003.

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ABSTRACT To elucidate the pathological roles of staphylococcal enterotoxin C (SEC) in bovine staphylococcal mastitis, a histopathological analysis of SEC-inoculated mammary glands was performed. SEC-inoculated mammary glands exhibited interstitial inflammation, and the leukocytes that migrated into the gland were predominantly polymorphonuclear neutrophils (PMN). In the gland cistern tissues dissected from SEC-inoculated mammary glands, epithelial cellular degeneration was observed. We also investigated the physiological effects of SEC on PMN in vitro. PMN migration was induced by culture supe
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44

Hill, J., and J. E. Samuel. "Coxiella burnetiiAcid Phosphatase Inhibits the Release of Reactive Oxygen Intermediates in Polymorphonuclear Leukocytes." Infection and Immunity 79, no. 1 (2010): 414–20. http://dx.doi.org/10.1128/iai.01011-10.

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ABSTRACTCoxiella burnetii, the etiological agent of Q fever, is a small, Gram-negative, obligate intracellular bacterium. Replication ofC. burnetiiduring infection has been shown to be increased by decreasing oxidative stress using p47phox −/−and iNOS−/−micein vivoand by pharmacologic inhibitorsin vitro. Building upon this model, we investigated the role polymorphonuclear leukocytes (PMN) play in the control of infection, since NADPH oxidase-mediated release of reactive oxygen intermediates (ROI) is a primary bactericidal mechanism for these cells that is critical for early innate clearance. E
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45

Berton, G., L. Fumagalli, C. Laudanna, and C. Sorio. "Beta 2 integrin-dependent protein tyrosine phosphorylation and activation of the FGR protein tyrosine kinase in human neutrophils." Journal of Cell Biology 126, no. 4 (1994): 1111–21. http://dx.doi.org/10.1083/jcb.126.4.1111.

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Stimulation of adherent human neutrophils (PMN) with tumor necrosis factor (TNF) triggers protein tyrosine phosphorylation (Fuortes, M., W. W. Jin, and C. Nathan. 1993. J. Cell Biol. 120:777-784). We investigated the dependence of this response on beta 2 integrins by using PMN isolated from a leukocyte adhesion deficiency (LAD) patient, which do not express beta 2 integrins, and by plating PMN on surface bound anti-beta 2 (CD18) antibodies. Protein tyrosine phosphorylation increased in PMN plated on fibrinogen and this phosphorylation was enhanced by TNF. Triggering of protein tyrosine phospho
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46

Schnur, R. A., and S. L. Newman. "The respiratory burst response to Histoplasma capsulatum by human neutrophils. Evidence for intracellular trapping of superoxide anion." Journal of Immunology 144, no. 12 (1990): 4765–72. http://dx.doi.org/10.4049/jimmunol.144.12.4765.

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Abstract Human neutrophils (PMN) have received little attention as to the role they play in host defense against Histoplasma capsulatum (Hc). We have characterized the binding and phagocytosis of Hc yeasts by human PMN and quantified the PMN respiratory burst in response to this organism. mAb specific for CD11a, CD11b, and CD11c all partially blocked the attachment of unopsonized yeasts to PMN; a mAb to CD18 inhibited attachment by greater than 90%. Thus, human PMN recognize and bind Hc yeasts via CD18 adhesion receptors as has been found for human cultured macrophages and alveolar macrophages
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47

Di Carlo, Andrea L., Max J. Paape, and Robert H. Miller. "Reactivity of purified complement component 3b with bovine neutrophils and modulation of complement receptor 1." American Journal of Veterinary Research 57, no. 2 (1996): 151–56. http://dx.doi.org/10.2460/ajvr.1996.57.02.151.

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Abstract Objective To study binding of purified complement component C3b to bovine blood and mammary neutrophils (PMN) after various treatments and determine their ability to modulate receptor numbers. Design Cell isolation, activation, and flow cytometric studies. Animals Healthy lactating Holstein cattle. Procedure Complement component C3b (18,300 kd) was isolated from bovine serum by column chromatography, and flow cytometric assays using fluorescein isothiocyanate-labeled C3b were developed to evaluate binding to PMN complement receptor 1. Multiple substances were tested to determine their
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48

Colotta, F., J. M. Wang, N. Polentarutti, and A. Mantovani. "Expression of c-fos protooncogene in normal human peripheral blood granulocytes." Journal of Experimental Medicine 165, no. 4 (1987): 1224–29. http://dx.doi.org/10.1084/jem.165.4.1224.

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We have investigated by Northern blot analysis the expression of c-fos protooncogene in human peripheral blood polymorphonuclear leukocytes (PMN). Freshly isolated PMN, unlike highly purified circulating lymphoid cells, showed high levels of c-fos transcripts. Appreciable c-fos mRNA was detected in monocytes, but in lesser amounts compared with PMN. Upon exposure to a series of agents that functionally stimulate granulocytes (PMA, inactivated streptococci, TNF, granulocyte and granulocyte/macrophage colony-stimulating factor), a considerable increase in c-fos transcripts was detected. Expressi
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49

van Kessel, K. P., J. A. van Strijp, and J. Verhoef. "Inactivation of recombinant human tumor necrosis factor-alpha by proteolytic enzymes released from stimulated human neutrophils." Journal of Immunology 147, no. 11 (1991): 3862–68. http://dx.doi.org/10.4049/jimmunol.147.11.3862.

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Abstract Activated human neutrophils (PMN) degrade rTNF-alpha resulting in a loss of cytotoxic activity against murine L-929 cells (L cells). This inactivation is mediated through proteases released from activated PMN. Exposure of TNF to H2O2, glucose oxidase, xanthine oxidase, or myeloper-oxidase-H2O2-halide did not affect TNF cytotoxicity for L cells. Exposure to trypsin, chymotrypsin, pronase E, or elastase, however, did diminish TNF bioactivity. FMLP-stimulated PMN in the presence, but not in the absence, of cytochalasin B reduced TNF activity, whereas PMA-stimulated PMN did not affect TNF
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50

Barnett, Carlton C., Ernest E. Moore, Gary W. Mierau, et al. "ICAM-1-CD18 interaction mediates neutrophil cytotoxicity through protease release." American Journal of Physiology-Cell Physiology 274, no. 6 (1998): C1634—C1644. http://dx.doi.org/10.1152/ajpcell.1998.274.6.c1634.

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Interaction of the β2-integrin complex on the polymorphonuclear neutrophil (PMN) with intercellular adhesion molecule-1 (ICAM-1) has been implicated in PMN-mediated cytotoxicity. This study examined interaction of the CD11a, CD11b, and CD18 subunits of the β2-integrin with ICAM-1, transfected into Chinese hamster ovarian (CHO) cells to avoid effects of other adhesion molecules. Incubation of quiescent PMNs with wild-type and ICAM-1-transfected CHO cells produced nominal cell lysis. Similarly, when phorbol myristate acetate (PMA)-activated PMNs were incubated with wild-type CHO cells, minimal c
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