To see the other types of publications on this topic, follow the link: PMPTS.
Journal articles on the topic 'PMPTS'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'PMPTS.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Noor, Syaharudin Shah Mohd, Muneera Esa, Ernawati Mustafa Kamal, and Aida Azlina Mansor. "Project manager personality traits towards project success: Moderated role of working experience in perspectives of small public construction projects in Malaysia." International Journal of Construction Supply Chain Management 10, no. 4 (December 31, 2020): 234–50. http://dx.doi.org/10.14424/ijcscm104020-234-250.
Full textAbstract:
Project success requires organisations and project managers to change strategies to satisfy stakeholders. Research into project success needs comprehensive analysis and approaches in various contexts, especially touching on non-technical skills (personality). This study aims to focus on examining the association of Project Manager Personality Traits (PMPTs) to the success of Small Public Construction Projects (SPCPs) and moderated by working experience. A survey was carried out to collect data using a structured Big Five Inventory (BFI) and Project Success Achieved Instrument (PSAI) questionnaire. Partial Least Squares Structural Equation Modeling (PLS-SEM) was used on a sample of 137 respondents for hypotheses testing and moderation effect analysis. Results show that PMPTs had a positive impact on the success of SPCPs. Conscientiousness (CT) and Agreeableness (AG) traits influence were more prominent when compared with other traits. While working experience does not moderate the relationship between PMPTs and the success of SPCPs. This study reflected the theoretical research of personality traits and their impact on the construction management industry. There have been limited studies of project success in the relationship with the BFI in the past, especially in the SPCPs context. The present study provides a basis for researchers interested in this area to examine further the use of BFI and PSAI as resources in other industries. Practically these findings may enable government or authorities to better align and suit project managers and their assigned project-based levels, where project managers are located in ministries overseeing small-scale projects. This study contributes theoretically to SPCPs literature by offering insights into project manager personality affecting project success and focus on selected agency in Malaysia.
2
Patkowski, A., J. Gapinski, T. Pakula, and G. Meier. "Physical nature of complex structural relaxation in polysiloxane – PMpTS: α and α′ relaxations." Polymer 47, no. 20 (September 2006): 7231–40. http://dx.doi.org/10.1016/j.polymer.2006.05.065.
Full text
3
Ker, De-Sheng, Sze Lei Pang, Noor Farhan Othman, Sekar Kumaran, Ee Fun Tan, Thiba Krishnan, Kok Gan Chan, Roohaida Othman, Maizom Hassan, and Chyan Leong Ng. "Purification and biochemical characterization of recombinant Persicaria minor β-sesquiphellandrene synthase." PeerJ 5 (February 28, 2017): e2961. http://dx.doi.org/10.7717/peerj.2961.
Full textAbstract:
Background Sesquiterpenes are 15-carbon terpenes synthesized by sesquiterpene synthases using farnesyl diphosphate (FPP) as a substrate. Recently, a sesquiterpene synthase gene that encodes a 65 kDa protein was isolated from the aromatic plant Persicaria minor. Here, we report the expression, purification and characterization of recombinant P. minor sesquiterpene synthase protein (PmSTS). Insights into the catalytic active site were further provided by structural analysis guided by multiple sequence alignment. Methods The enzyme was purified in two steps using affinity and size exclusion chromatography. Enzyme assays were performed using the malachite green assay and enzymatic product was identified using gas chromatography-mass spectrometry (GC-MS) analysis. Sequence analysis of PmSTS was performed using multiple sequence alignment (MSA) against plant sesquiterpene synthase sequences. The homology model of PmSTS was generated using I-TASSER server. Results Our findings suggest that the recombinant PmSTS is mainly expressed as inclusion bodies and soluble aggregate in the E. coli protein expression system. However, addition of 15% (v/v) glycerol to the protein purification buffer and removal of N-terminal 24 amino acids of PmSTS helped to produce homogenous recombinant protein. Enzyme assay showed that recombinant PmSTS is active and specific to the C15 substrate FPP. The optimal temperature and pH for the recombinant PmSTS are 30 °C and pH 8.0, respectively. The GC-MS analysis further showed that PmSTS produces β-sesquiphellandrene as a major product and β-farnesene as a minor product. MSA analysis revealed that PmSTS adopts a modified conserved metal binding motif (NSE/DTE motif). Structural analysis suggests that PmSTS may binds to its substrate similarly to other plant sesquiterpene synthases. Discussion The study has revealed that homogenous PmSTS protein can be obtained with the addition of glycerol in the protein buffer. The N-terminal truncation dramatically improved the homogeneity of PmSTS during protein purification, suggesting that the disordered N-terminal region may have caused the formation of soluble aggregate. We further show that the removal of the N-terminus disordered region of PmSTS does not affect the product specificity. The optimal temperature, optimal pH, Km and kcat values of PmSTS suggests that PmSTS shares similar enzyme characteristics with other plant sesquiterpene synthases. The discovery of an altered conserved metal binding motif in PmSTS through MSA analysis shows that the NSE/DTE motif commonly found in terpene synthases is able to accommodate certain level of plasticity to accept variant amino acids. Finally, the homology structure of PmSTS that allows good fitting of substrate analog into the catalytic active site suggests that PmSTS may adopt a sesquiterpene biosynthesis mechanism similar to other plant sesquiterpene synthases.
4
Yang, Yi, Tian-Ling Ren, Yi-Ping Zhu, Xiao-Ming Wu, Ning-Xin Zhang, and Li-Tian Liu. "PMUTs for Handwriting Recognition." Integrated Ferroelectrics 69, no. 1 (January 1, 2005): 341–47. http://dx.doi.org/10.1080/10584580590899306.
Full text
5
Alasatri, Suresh, Libor Rufer, and Joshua En-Yuan Lee. "AlN-on-Si Square Diaphragm Piezoelectric Micromachined Ultrasonic Transducer with Extended Range of Detection." Proceedings 2, no. 13 (November 27, 2018): 913. http://dx.doi.org/10.3390/proceedings2130913.
Full textAbstract:
We present aluminum nitride (AlN) on silicon (Si) CMOS-compatible piezoelectric micromachined ultrasonic transducers (pMUTs) with an extended detection range of up to 140 cm for touchless sensing applications. The reported performance surpasses the current state-of-art for AlN-based pMUTs in terms of the maximum range of detection using just a pair of pMUTs (as opposed to an array of pMUTs). The extended range of detection has been realized by using a larger diaphragm allowed by fabricating a thicker diaphragm than most other pMUTs reported to date. Using a pair of pMUTs, we experimentally demonstrate the capability of range-finding by correlating the time-of-flight (TOF) between the transmit (TX) and receive (RX) pulse. The results were obtained using an experimental setup where the MEMS chip was interconnected with a customized printed circuit board (PCB) using Al wire bonds.
6
Crainic, Marius, Rui Loja Fernandes, and David Martínez Torres. "Poisson manifolds of compact types (PMCT 1)." Journal für die reine und angewandte Mathematik (Crelles Journal) 2019, no. 756 (November 1, 2019): 101–49. http://dx.doi.org/10.1515/crelle-2017-0006.
Full textAbstract:
AbstractThis is the first in a series of papers dedicated to the study of Poisson manifolds of compact types (PMCTs). This notion encompasses several classes of Poisson manifolds defined via properties of their symplectic integrations. In this first paper we establish some fundamental properties and constructions of PMCTs. For instance, we show that their Poisson cohomology behaves very much like the de Rham cohomology of a compact manifold (Hodge decomposition, non-degenerate Poincaré duality pairing, etc.) and that the Moser trick can be adapted to PMCTs. More important, we find unexpected connections between PMCTs and symplectic topology: PMCTs are related with the theory of Lagrangian fibrations and we exhibit a construction of a non-trivial PMCT related to a classical question on the topology of the orbits of a free symplectic circle action. In subsequent papers, we will establish deep connections between PMCTs and integral affine geometry, Hamiltonian G-spaces, foliation theory, orbifolds, Lie theory and symplectic gerbes.
7
Sun, J. T., and Y. D. Huang. "Synthesis and Properties of Poly(Methylphenylsiloxane) Containing Methacryloyl Groups." Polymers and Polymer Composites 15, no. 6 (September 2007): 463–68. http://dx.doi.org/10.1177/096739110701500605.
Full textAbstract:
Methacryloxypropyltrimethoxysilane (MPTS) was used as the modified monomer to synthesise polymethylphenylsiloxane containing methacryloyl groups (PMPS-M) through co-hydrolysis and polycondensation with other silane monomers. The effects of MPTS on the synthesis and on the thermal and adhesive properties of PMPS-M were investigated by using Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), differential thermogravimetry (DTG), scanning electron microscopy (SEM), and measurements of adhesion using a shear test. The results indicated that PMPS-M with good storage stability was synthesised successfully when the amount of MPTS was less than 8 wt%. PMPS-M had higher thermal stability than PMPS. The onset degradation temperature for PMPS-M containing 8 wt% MPTS was 518 °C, while it was 463 °C for PMPS. The improvement in thermal stability may be due to the protective effect of the methacryloyl groups, as observed under SEM. The shear strength of PMPS-M was 4.2 MPa when containing about 8 wt% MPTS, about 50% higher than that of PMPS. The shear strength of PMPS-M was also higher than that of PMPS at high temperatures.
8
Osumi, Kazuoki, Yasushi Ozeki, Sonoko Saito, Yoshie Nagamura, Hideki Ito, Yukio Kimura, Hiroshi Ogura, and Shosaku Nomura. "Development and Assessment of Enzyme Immunoassay for Platelet-derived Microparticles." Thrombosis and Haemostasis 85, no. 02 (2001): 326–30. http://dx.doi.org/10.1055/s-0037-1615688.
Full textAbstract:
SummaryPlatelet-derived microparticles (PMPs) are released from platelets through the platelet activation by high shear stress, collagen, or calcium ionophore (A23187). PMPs are observed in patients with acute myocardial infarction, thrombotic thrombocytopenic purpura, hemolytic uremic syndrome, heparin-induced thrombocytopenia and other thrombotic disorders, but the importance of circulating PMPs in the pathogenesis of these diseases is still debated. Numbers of PMPs are usually determined by flowcytometry (FCM), but easier and reproducible PMP assay systems are needed. To develop a better ELISA for PMPs, we used antibodies against the platelet antigens anti-GPIb (NNKY5-5), anti-GPIIb/IIIa (NNKY2-11, anti-CD41), anti-GPIX (KMP-9), and anti-CD9 (NNKY1-19). PMPs were detected with all combinations of these antibodies, but the ELISA having the highest and most specific absorbance was obtained with a combination of KMP-9 (capture antibody) and NNKY5-5 (detecting antibody). PMPs in blood samples were measured by ELISA and FCM. ELISA correlated with PMPs quantitated by FCM. By shaking ELISA plates during incubation, nonspecific binding of platelets was eliminated. The level of PMPs was not increased in diabetes mellitus, thrombotic thrombocytopenic purpura, anti-phospholipid syndrome, or sepsis. The concentration of PMP was elevated in hemolytic uremic syndrome. Activated PMPs were absorbed to 0.8 m filter, but circulating PMPs were not absorbed. These results suggest that activated PMPs are likely to adhere to leukocytes or endothelial cells at the activation site and that the circulating form of PMPs are likely to be a residue of activated PMPs. To detect only the activated form of PMPs, a new ELISA needs to be developed, and it will likely use a combination of antibodies that detect platelet activation markers such as P-selectin (CD62P) or activated GPIIb/IIIa.
9
Chen, Bao-An, Yue-Jiao Zhong, Cheng-Yin Huang, Feng Gao, Jian Chen, Chong Gao, Yun-Yu Sun, et al. "Platelet-Derived Microparticles Induce Angiogenesis In Vitro and In Vivo." Blood 110, no. 11 (November 16, 2007): 3901. http://dx.doi.org/10.1182/blood.v110.11.3901.3901.
Full textAbstract:
Abstract Objective: An attempt was made to investigate the effect of platelet-derived microparticles (PMPs) on the angiogenesis. Methods: Thrombin was adopted to activate the platelets to release PMPs. Flow cytometry(FCM)was adopted to evaluate the efficiencies of different concentrations of thrombin to produce PMPs and BCA was adopted to evaluate the content of PMPs. By the carrier of human umbilical vein cell line ECV-304 cultivated in vitro, investigate the effect of PMPs on the proliferation and apoptosis of human umbilical vein endothelial cells using MTT and FCM. PMPs were put into CAM and observe the effects of PMPs on angiogenesis in Chick embryo chorioallantoic membrane (CAM). Results: The efficiencies of PMPs activated by 1.0U/ml thrombin were 50.1%; PMPs induced proliferation of human umbilical vein cell line ECV-304 in a dose dependent manner. At the concentration of 40ug/ml PMPs, the proliferation rate of human umbilical vein cell line ECV-304 was as 1.8±0.3 times as control and there was no difference with the group of vascular endothelial growth factor (VEGF),which the proliferation rate was 1.9±0.5 times vs. control, p > 0.05;PMPs inhibited human umbilical vein cell line ECV-304 apoptosis. Compared with control group (apoptosis rate 9.4%±0.5%), apoptosis rate of PMPs (40μg/ml) is 3.9%±0.4%, which was significantly reduced, p<0.05. The addition of VEGF (10μl/ml) did not successfully prevented apoptosis of human umbilical vein cell line ECV-304 (apoptosis rate 8.0%±0.8%);After 72 h of incubation, showing an implant of PMPs by allantoic vessels developing radially towards it (implant) in a ’spoked-wheel’ pattern, at the concentration of 80μg/ml PMPs, number of vessel ramification is 112.5±11.31 and vessel area/CAM area is (6.19±1.29)%, compared with the VEGF(p>0.05). But there are not localized allantoic vessels developing in the NS control group(P<0.05). Conclusion: 1.0U/ml thrombin activated platelet could get the best efficiency of PMPs, which could stimulate proliferation of human umbilical vein cell line ECV-304 and inhibit its apoptosis and PMPs have certain promotive effect on the formation of capillary in chick chorioallantoic membranes.
10
Kawasugi, Kazuo, Mizuho Noguchi, Haruko Tashiro, Moritaka Gotoh, Naoki Shirafuji, Juzo Matsuda, Yukihisa Miyazawa, and Tadatoshi Kinoshita. "Increased Levels of Platelet-Derived Microparticles in Patients with DIC." Blood 106, no. 11 (November 16, 2005): 4083. http://dx.doi.org/10.1182/blood.v106.11.4083.4083.
Full textAbstract:
Abstract Circulating microparticles of various cell types are present in healthy individuals and individuals in various disease states. In these microparticles, platelet-dreived microparticles (PMPs) may be implicated in thrombosis and haemostasis. However, little is known about the role of PMPs in patients with DIC. To investigate the role of PMPs, we measured plasma levels of PMPs in patients with sepsis-associated DIC (n=20) and acute promyelocytic leukemia (APL)-induced DIC (n=5). Plasma samples from those patients groups were assayed for PMPs levels by enzyme-linked immunosorbent assay (ELISA, Japan Immunoresearch Laboratory, Japan). Also, sP-selectin levels were determined by ELISA. The thrombin antithrombin complexes (TAT) levels were higher in both types of DIC patients as reported by others. In the septic patients with DIC, we found significant elevation in PMPs levels as compared with normal controls. However, we did not find any difference in plasma levels of PMPs in the APL patients with DIC. The sP-selectin levels were elevated in the septic patients with DIC. In early phase of septic patients with DIC, plasma levels of PMPs tend to be higher than that in late phase of septic patients with DIC.These results suggest that plasma levels of PMPs may be candidates for a marker of septic DIC. It appears that PMPs may contribute to the processes of septic DIC. Sepsis associated DIC APL with DIC Controls (n=20) *(P<0.05) significantly different controls PMPs 34.1 ± 50.1* 9.6 ± 3.6 6.5 ± 0.9 U/ml TAT 25.4 ± 15.5* 22.5 ± 15.3* <;3 μ g/ml
11
Sacksteder, Katherine A., Jacob M. Jones, Sarah T. South, Xiaoling Li, Yifei Liu, and Stephen J. Gould. "Pex19 Binds Multiple Peroxisomal Membrane Proteins, Is Predominantly Cytoplasmic, and Is Required for Peroxisome Membrane Synthesis." Journal of Cell Biology 148, no. 5 (March 6, 2000): 931–44. http://dx.doi.org/10.1083/jcb.148.5.931.
Full textAbstract:
Peroxisomes are components of virtually all eukaryotic cells. While much is known about peroxisomal matrix protein import, our understanding of how peroxisomal membrane proteins (PMPs) are targeted and inserted into the peroxisome membrane is extremely limited. Here, we show that PEX19 binds a broad spectrum of PMPs, displays saturable PMP binding, and interacts with regions of PMPs required for their targeting to peroxisomes. Furthermore, mislocalization of PEX19 to the nucleus leads to nuclear accumulation of newly synthesized PMPs. At steady state, PEX19 is bimodally distributed between the cytoplasm and peroxisome, with most of the protein in the cytoplasm. We propose that PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane. This hypothesis is supported by the observation that the loss of PEX19 results in degradation of PMPs and/or mislocalization of PMPs to the mitochondrion.
12
Tan, Kiat T., and Gregory Y. H. Lip. "The potential role of platelet microparticles in atherosclerosis." Thrombosis and Haemostasis 94, no. 09 (2005): 488–92. http://dx.doi.org/10.1160/th05-03-0201.
Full textAbstract:
SummaryThe release of microvesicles (‘platelet microparticles’, PMPs) by activated platelets has been shown to be an integral part of the thrombotic process. PMPs are believed to mediate many biological processes as they possess various platelet membrane proteins and bioactive lipids. Of note, there is a growing body of evidence that PMPs are involved in all stages in the pathobiology of atherosclerosis. In addition to their role in thrombosis, PMPs may also have a pro-inflammatory effect, which promotes the development of atherosclerosis. Also, bioactive lipids in PMPs have been shown to have important effects on angiogenesis. This review summarises the various studies on the possible role of PMPs in the progression of atherosclerosis.
13
Chen, Bao-An, Fei Fei, Cheng-Yin Huang, Cui-Ping Li, Xiao-Ping Pei, Chong Gao, Jia-Hua Ding, Yun-Yu Sun, Feng Gao, and Ning-Na Chen. "Platelet-Derived Microparticels Stimulate Proliferation of Colony-Forming Unit Granulocyte-Macrophage of Umbilical Cord Blood Hematopoietic Stem Cells." Blood 106, no. 11 (November 16, 2005): 4191. http://dx.doi.org/10.1182/blood.v106.11.4191.4191.
Full textAbstract:
Abstract Umbilical Cord blood has become an important source of hematopoietic stem-progenitor cells for transplantation, however hematopoietic recovery after transplantation with umbilical cord blood is slower than with bone marrow or mobilized peripheral blood. Adhesion molecules on hematopoietic cells are involved in hematopoietic cells’ homing, which is considered the most important step of hematological recovery. Some articles indicated that expressions of adhesion molecules on CD34+ cells could predict the time to hematopoietic recovery after transplantation with bone marrow and peripheral blood of many adhesion molecules (such as CD62, CXCR4) are significantly lower on umbilical cord blood than on bone marrow. It is a possible reason for the difficulty in hematopoietic recovery after umbilical cord blood transplant. Platelet -derived microparticles (PMPs) are submicroscopic (<1 μm) membrane vesicles released from platelet if they are stimulated with agonists such as thrombin, collagen, or calcium ionophore A23187 or if exposed to high-stress shear forces. PMPs express several platelet-endothelium attachment receptors on their surface, for example, glycoprotein IIb/IIIa (CD41), Ib and IaIIa, and P-selectin (CD62P) and several other platelet relevant receptors such as CXCR4 and PAR-1. Some articles indicate that PMPs can affect the function of hematopoietic stem cells by increasing the adhesion of hematopoietic cells to fibrinogen, which suggests that PMP-transferred CD41 antigen plays an important role in this process. PMPs can also increase the survival of human hematopoietic cells including human CD34+ clonogenic progenitors. In our research, we observe the function of PMP to affect the cloning efficiency of colony-forming unit granulocyte-macrophage (CFU-GM). We adopt different concentrations of Thrombin (2U/ml, 1.5U/ml, 1.0U/ml and 0.5U/ml) to activate the platelet and acquire PMPs. Then PMPs were evaluated by using flow cytometry. Based on the result that stimulation of platelets by Thrombin (1U/ml) can acquire the best efficiency of PMPs, we used this concentration in all subsequent experiments. Umbilical cord mononuclear cells (MNCs) were obtained from healthy donors and isolate the MNCs by Ficoll-Hypaque density gradient centrifugation. Briefly, MNCs incubated with or without PMPs cultured in 2.7% methylcellulose. CFU-GM growth was stimulated with 30% umbilical cord serum, rhIL-3 and rh GM-CSF. Cultures were incubated at 37°C in a fully humidified atmosphere supplemented with 5% CO2. Colonies were counted under an inverted microscope after 7 or 10 days. The research was divided into four groups: 1. control group; 2. PMPs(10μg/ml); 3. PMPs(50μg/ml); 4. PMPs(100μg/ml). The colony formation was enhanced with PMPs and is dependently stimulated with PMPs. The number of colonies in the group of PMPs(100μg/ml) is more than that of other groups. The number of colonies in control group, PMPs(10μg/ml), PMPs(50μg/ml) and PMPs(100μg/ml) are 57.4±3.2, 65.6±5.6, 77.1±1.7 and 87.8±5.0 per 1×105 respectively. These increases in different groups were statistically significant when compared with control group(p<0.05). To sum up, PMPs can affect the cloning efficiency of CFU-GM of umbilical cord hematopoietic stem cells and the efficiencies are depended on the concentration of PMPs.
14
Miguel, Rafael, Ann M. Kuhn, Alan R. Shons, Patricia Dyches, Mark D. Ebert, Eric S. Peltz, Keoni Nguyen, and Charles E. Cox. "The Effect of Sentinel Node Selective Axillary Lymphadenectomy on the Incidence of Postmastectomy Pain Syndrome." Cancer Control 8, no. 5 (September 2001): 427–30. http://dx.doi.org/10.1177/107327480100800506.
Full textAbstract:
Background Postmastectomy pain syndrome (PMPS) has been reported following procedures involving complete lymph node dissection (CLND). Since the triggering event is probably related to nerve injury, sentinel lymph node dissection (SLND) should decrease the incidence of PMPS. The purpose of this report is to determine the impact of SLND on the number of patients referred to the pain clinic for PMPS treatment. Methods The records of all breast surgical patients with a diagnosis of PMPS referred to the Moffitt Cancer Center pain clinic were reviewed. The criterion for diagnosis of PMPS was a history of postoperative pain in the upper anterior chest wall, upper extremity, axilla, and/or shoulder in the absence of recurrent disease. Results A total of 55 patients with a diagnosis of PMPS were seen in the pain clinic since 1991. Treatments included local anesthetics/corticosteroid injection, stellate ganglion block, and tricyclic antidepressants. A decrease from 15 patients in 1991 to 3 in 1998 was observed. All but one of the 55 patients with PMPS had CLND, and none referred to the pain clinic had undergone SLND. Conclusions PMPS is a complication of CLND. The increased use of SLND in our center has reduced the number of referrals to the pain clinic for treatment of PMPS. This benefit of SLND reduces suffering in the postoperative breast patient.
15
Janowska-Wieczorek, Anna, Marcin Majka, Jacek Kijowski, Monika Baj-Krzyworzeka, Ryan Reca, A. Robert Turner, Janina Ratajczak, Steven G. Emerson, M. Anna Kowalska, and Mariusz Z. Ratajczak. "Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment." Blood 98, no. 10 (November 15, 2001): 3143–49. http://dx.doi.org/10.1182/blood.v98.10.3143.h8003143_3143_3149.
Full textAbstract:
Because human CD34+ and murine Sca-1+hematopoietic stem–progenitor cells (HSPCs) express platelet-binding sialomucin P-selectin (CD162) and integrin Mac-1 (CD11b–CD18) antigen, it was inferred that these cells might interact with platelets. As a result of this interaction, microparticles derived from platelets (PMPs) may transfer many platelet antigens (CD41, CD61, CD62, CXCR4, PAR-1) to the surfaces of HSPCs. To determine the biologic significance of the presence of PMPs on human CD34+ and murine Sca-1+ cells, their expressions on mobilized peripheral blood (mPB) and on nonmobilized PB- and bone marrow (BM)–derived CD34+ cells were compared. In addition, the effects of PMPs on the proliferation of CD34+ and Sca-1+ cells and on adhesion of HSPCs to endothelium and immobilized SDF-1 were studied. Finally, the hematopoietic reconstitution of lethally irradiated mice receiving transplanted BM mononuclear cells covered or not covered with PMPs was examined. It was found that PMPs are more numerous on mPB than on BM CD34+cells, do not affect the clonogenicity of human and murine HSPCs, and increase adhesion of these cells to endothelium and immobilized SDF-1. Moreover, murine BM cells covered with PMPs engrafted lethally irradiated mice significantly faster than those not covered, indicating that PMPs play an important role in the homing of HSPCs. This could explain why in a clinical setting human mPB HSPCs (densely covered with PMPs) engraft more rapidly than BM HSPCs (covered with fewer PMPs). These findings indicate a new role for PMPs in stem cell transplantation and may have clinical implications for the optimization of transplantations.
16
Lehmann, A., A. Britting, W. Eyrich, and F. Uhlig. "Lifetime of MCP-PMTs." Journal of Instrumentation 9, no. 02 (February 11, 2014): C02009. http://dx.doi.org/10.1088/1748-0221/9/02/c02009.
Full text
17
Lehmann, A., A. Britting, W. Eyrich, M. Pfaffinger, F. Uhlig, A. Belias, R. Dzhygadlo, et al. "Lifetime of MCP-PMTs." Journal of Instrumentation 11, no. 05 (May 10, 2016): C05009. http://dx.doi.org/10.1088/1748-0221/11/05/c05009.
Full text
18
Zhou, Qian, Yan Lian, Yan Zhang, Lei Li, Hongyan Li, Di Shen, Yu Zhou, et al. "Platelet-derived microparticles from recurrent miscarriage associated with antiphospholipid antibody syndrome influence behaviours of trophoblast and endothelial cells." Molecular Human Reproduction 25, no. 8 (April 3, 2019): 483–94. http://dx.doi.org/10.1093/molehr/gaz019.
Full textAbstract:
AbstractPlatelet-derived microparticles (PMPs) are a type of microparticle budding from platelets undergoing activation or apoptosis in many autoimmune diseases, including antiphospholipid antibody syndrome (APS). PMPs may also contribute to recurrent miscarriage, although the exact mechanism is unclear. The aim of this study was to determine the potential biological mechanism by which abnormal PMP activation may affect recurrent miscarriage. PMPs were counted by fluorescence-activated cell sorting (FACS) and compared between the healthy control (HC) and recurrent miscarriage/APS groups. Different effects of PMPs isolated by FACS from patients with recurrent miscarriage/APS and HCs were explored. Capillary electrophoresis immunoquantification, RT-qPCR, Luminex xMAP and immunofluorescence staining were performed to investigate all these different effects of PMPs. We found that the difference in the counts of PMP was not significant. However the expression of the inflammatory cytokine tumour necrosis factor-α (TNF-α) and the adhesion molecules intracellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were increased by PMPs derived from the recurrent miscarriage/APS group. PMPs isolated from patients with recurrent miscarriage/APS also more potently stimulated monocyte recruitment, inhibited angiogenesis and promoted human umbilical vein endothelial cell (HUVEC) apoptosis, in comparison to PMPs from HCs matched for gestational week. Moreover, PMPs could be ternalized by HTR-8/SVneo cells and could increase apoptosis of these cells and decrease trophoblastic invasion and migration. To supplement our work, the limited sample size needs to be increased, and further in-vivo work is necessary. Findings from this study indicate that abnormal activation of PMPs contributes to recurrent miscarriage/APS progression and provides potential therapeutic targets.
19
Janowska-Wieczorek, Anna, Marcin Majka, Jacek Kijowski, Monika Baj-Krzyworzeka, Ryan Reca, A. Robert Turner, Janina Ratajczak, Steven G. Emerson, M. Anna Kowalska, and Mariusz Z. Ratajczak. "Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment." Blood 98, no. 10 (November 15, 2001): 3143–49. http://dx.doi.org/10.1182/blood.v98.10.3143.
Full textAbstract:
Abstract Because human CD34+ and murine Sca-1+hematopoietic stem–progenitor cells (HSPCs) express platelet-binding sialomucin P-selectin (CD162) and integrin Mac-1 (CD11b–CD18) antigen, it was inferred that these cells might interact with platelets. As a result of this interaction, microparticles derived from platelets (PMPs) may transfer many platelet antigens (CD41, CD61, CD62, CXCR4, PAR-1) to the surfaces of HSPCs. To determine the biologic significance of the presence of PMPs on human CD34+ and murine Sca-1+ cells, their expressions on mobilized peripheral blood (mPB) and on nonmobilized PB- and bone marrow (BM)–derived CD34+ cells were compared. In addition, the effects of PMPs on the proliferation of CD34+ and Sca-1+ cells and on adhesion of HSPCs to endothelium and immobilized SDF-1 were studied. Finally, the hematopoietic reconstitution of lethally irradiated mice receiving transplanted BM mononuclear cells covered or not covered with PMPs was examined. It was found that PMPs are more numerous on mPB than on BM CD34+cells, do not affect the clonogenicity of human and murine HSPCs, and increase adhesion of these cells to endothelium and immobilized SDF-1. Moreover, murine BM cells covered with PMPs engrafted lethally irradiated mice significantly faster than those not covered, indicating that PMPs play an important role in the homing of HSPCs. This could explain why in a clinical setting human mPB HSPCs (densely covered with PMPs) engraft more rapidly than BM HSPCs (covered with fewer PMPs). These findings indicate a new role for PMPs in stem cell transplantation and may have clinical implications for the optimization of transplantations.
20
Usuda, N., T. Kuwabara, R. Ichikawa, T. Hashimoto, and T. Nagata. "Immunoelectron microscopic evidence for organ differences in the composition of peroxisome-specific membrane polypeptides among three rat organs: liver, kidney, and small intestine." Journal of Histochemistry & Cytochemistry 39, no. 10 (October 1991): 1357–66. http://dx.doi.org/10.1177/39.10.1940307.
Full textAbstract:
We examined the distribution of peroxisome-specific membrane polypeptides (PMPs) among peroxisomes of the liver, renal cortex, and jejunal mucosa, using antibodies for 70 KD, 26 KD and 22 KD PMPs. Immunoblot analysis showed signals for 70 KD polypeptide in all three kinds of tissue, but for the other two only in the liver and renal cortex, with neither being detected in jejunal mucosa. The total amounts of PMPs increased in all three organs with DEHP (di-(2-ethylhexyl)phthalate) administration. By immunoelectron microscopic analysis using protein A-gold, the three PMPs were localized along the peroxisomal membrane. Quantitation of the gold particles associated with the peroxisomal membrane showed an increase in the density of 70 KD and 26 KD PMPs but a decrease in 22 KD PMP with the administration of DEHP. The presence of tissue-specific localizations of PMPs suggest the 70 KD PMP is a common constituent of peroxisomes of these three tissues, whereas 26 KD and 22 KD PMPs are absent in microperoxisomes of jejunal mucosal epithelium.
21
Ee, Su-Fang, Zeti-Azura Mohamed-Hussein, Roohaida Othman, Noor Azmi Shaharuddin, Ismanizan Ismail, and Zamri Zainal. "Functional Characterization of Sesquiterpene Synthase fromPolygonum minus." Scientific World Journal 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/840592.
Full textAbstract:
Polygonum minusis an aromatic plant, which contains high abundance of terpenoids, especially the sesquiterpenes C15H24. Sesquiterpenes were believed to contribute to the many useful biological properties in plants. This study aimed to functionally characterize a full length sesquiterpene synthase gene fromP. minus.P. minussesquiterpene synthase (PmSTS) has a complete open reading frame (ORF) of 1689 base pairs encoding a 562 amino acid protein. Similar to other sesquiterpene synthases, PmSTS has two large domains: the N-terminal domain and the C-terminal metal-binding domain. It also consists of three conserved motifs: the DDXXD, NSE/DTE, and RXR. A three-dimensional protein model for PmSTS built clearly distinguished the two main domains, where conserved motifs were highlighted. We also constructed a phylogenetic tree, which showed that PmSTS belongs to the angiosperm sesquiterpene synthase subfamily Tps-a. To examine the function ofPmSTS, we expressed this gene inArabidopsis thaliana. Two transgenic lines, designated asOE3andOE7, were further characterized, both molecularly and functionally. The transgenic plants demonstrated smaller basal rosette leaves, shorter and fewer flowering stems, and fewer seeds compared to wild type plants. Gas chromatography-mass spectrometry analysis of the transgenic plants showed that PmSTS was responsible for the production ofβ-sesquiphellandrene.
22
Kailashiya, Jyotsna. "Biosensor Designs for Platelet-derived Microparticles Analysis." Annals of the National Academy of Medical Sciences (India) 53, no. 04 (October 2017): 234–37. http://dx.doi.org/10.1055/s-0040-1712812.
Full textAbstract:
ABSTRACTPlatelet-derived microparticles (PMPs) are often used as marker of platelet activation and their count in blood has been found to be significantly associated with many diseases like myocardial infarction, stroke, venous thrombo-embolism etc. PMPs have been proposed as potential biomarkers for these conditions. Biosensors are newer analytical tools, being developed for convenient and cost effective analysis. For PMPs analysis, biosensors offer many advantages over conventional analysis techniques. This mini review compiles designs and techniques of reported biosensors based on antibody capturing for analysis of PMPs.
23
Silakul, Pensiri, and Rathanawan Magaraphan. "Gel Polymer Electrolyte from Ozonolysis of Poly(3-(trimethoxysilyl)Propyl Methacrylate) Graft on Natural Rubber Latex for Natural Dye Sensitized Solar Cell Application." Advanced Materials Research 844 (November 2013): 357–60. http://dx.doi.org/10.4028/www.scientific.net/amr.844.357.
Full textAbstract:
The dye sensitized solar cell (DSSC) is devices for converting solar energy to electricity. The gel polymer electrolyte is the main component in the cell. It is used for complexation with liquid electrolyte to generate iodide ions and support the ion transportation. The gel polymer electrolyte of poly(3-(trimethoxysilyl) propyl methacrylate) (PMPS) coat on the surfaces of natural rubber (NR) particles (PMPS-g-NR) was prepared by surface grafting emulsion polymerization method using the redox couple initiator, tetraethylenepentamine (TEPA) and cumenehydroperoxide (CHP). Then, this gel was treated by ozone at different treatment time (5 min, 10 min, 20 min, 25 min, and 30 min) and voltage of ozonolysis (0.7 kV, 1.5 kV, 2.4 kV, and 3.2 kV) on PMPS-g-NR (ozonolysed PMPS-g-NR) to obtain the optimum condition for generating the active species e.g. carbonyl or aldehyde structures. The synthesized materials were characterized by the degree of swelling (%DS), ionic conductivity, and solar cell efficiency, having red cabbage as dye sensitizer. By ozonolysis on PMPS-g-NR, the treatment condition of voltage at 3.2 kV and treatment time at 30 min showed the maximum %DS of 74.2, which is higher than that of PMPS-g-NR without ozonolysis of 65.5. The maximum ion conductivity of PMPS-g-NR and ozonolysed PMPS-g-NR at 2.4 kV and 20 min were 0.240 mS/cm and 0.775 mS/cm, respectively while their maximum solar cell efficiency was 6.738 m% and 10.340 m%, respectively.
24
Shang, Yu Cheng, Shota Yamada, Ya Dong Chai, and Motohiro Tagaya. "Synthesis of Spherical Phosphorus-Containing Mesoporous Silica for Improving their Reaction Behavior in Simulated Body Fluid." Key Engineering Materials 782 (October 2018): 59–64. http://dx.doi.org/10.4028/www.scientific.net/kem.782.59.
Full textAbstract:
Spherical phosphorus–containing mesoporous silica (PMPS) particles were synthesized. In the PMPS particle preparation using the cationic surfactant (cetyltrimethylammonium bromide) as the template, the amphiphilic surfactant (Pluronic P123) and diethyl(2–bromoethyl)phosphonate were used for the particle shape control and the phosphorus source, respectively. Furthermore, we investigated the chemical reactions of the PMPS particles in simulated body fluid (SBF). By the phosphorus–containing, the hydroxyapatite formation and silicate ion dissolution ability on the PMPS particle surfaces were enhanced. These characteristic features will be useful for the biomedical applications such as bone formation.
25
Miao, Di, Tian-Tian Ma, Min Chen, and Ming-Hui Zhao. "Platelets release proinflammatory microparticles in anti-neutrophil cytoplasmic antibody-associated vasculitis." Rheumatology 58, no. 8 (March 6, 2019): 1432–42. http://dx.doi.org/10.1093/rheumatology/kez044.
Full textAbstract:
Abstract Objective The biological functions of the platelets contributing to ANCA-associated vasculitis (AAV) are largely unclear. The current study aimed to investigate the potential role of platelet-derived microparticles (PMPs) in AAV. Methods In the current study, microparticles in AAV patients were analysed by flow cytometry, and PMPs were probed for relative levels of 640 bioactive proteins secreted from patients’ platelets using antibody microarrays. These data were then correlated with clinical and pathological parameters. Results PMPs were significantly increased in 69 AAV patients, predominantly MPO-ANCA positive patients in active stage compared with in remission [4406.8/μl (2135.4, 5485.0) vs 549.7/μl (350, 708.5), P < 0.0001], and 43% of microparticles in active AAV were PMPs. Compared with 15 patients in remission, highly expressed proinflammatory molecules in the microparticles from platelets in 15 AAV patients in active stage revealed that potential functions of PMPs were promotion of the effect of chemotaxis, adhesion, growth and apoptosis (all the patients for array analysis were MPO-ANCA positive). The level of PMPs had a significant association with disease activity, inflammation, and renal damage. Conclusion PMPs may serve as inflammatory propagators through their wide production of proinflammatory cytokines in AAV, potentially providing a novel therapeutic target.
26
Cacic, Daniel, Håkon Reikvam, Oddmund Nordgård, Peter Meyer, and Tor Hervig. "Platelet Microparticles Protect Acute Myelogenous Leukemia Cells against Daunorubicin-Induced Apoptosis." Cancers 13, no. 8 (April 14, 2021): 1870. http://dx.doi.org/10.3390/cancers13081870.
Full textAbstract:
The role of platelets in cancer development and progression is increasingly evident, and several platelet–cancer interactions have been discovered, including the uptake of platelet microparticles (PMPs) by cancer cells. PMPs inherit a myriad of proteins and small RNAs from the parental platelets, which in turn can be transferred to cancer cells following internalization. However, the exact effect this may have in acute myelogenous leukemia (AML) is unknown. In this study, we sought to investigate whether PMPs could transfer their contents to the THP-1 cell line and if this could change the biological behavior of the recipient cells. Using acridine orange stained PMPs, we demonstrated that PMPs were internalized by THP-1 cells, which resulted in increased levels of miR-125a, miR-125b, and miR-199. In addition, co-incubation with PMPs protected THP-1 and primary AML cells against daunorubicin-induced cell death. We also showed that PMPs impaired cell growth, partially inhibited cell cycle progression, decreased mitochondrial membrane potential, and induced differentiation toward macrophages in THP-1 cells. Our results suggest that this altering of cell phenotype, in combination with decrease in cell activity may offer resistance to daunorubicin-induced apoptosis, as serum starvation also yielded a lower frequency of dead and apoptotic cells when treated with daunorubicin.
27
Qiang, Tao Tao, Xiang Luo, Long Fang Ren, Xue Chuan Wang, and Bao Yuan He. "Preparation, Characterization and Application of Siloxane Succinate Fatliquor." Advanced Materials Research 233-235 (May 2011): 292–97. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.292.
Full textAbstract:
The silicone succinate surfactant (PMPS) was synthesized by ring-opening reaction using polyether alcohol amine modified polysilosane (PAPS) with maleic anhydride (MA) as the raw materials, and p-toluenesulfonic acid (PTSA) was used as the catalyst. Sulfated castor oil and sulfated rapeseed oil were combinated with PMPS to prepare a new leather fatliqour (PMPSF) composed mainly of PMPS. The molecular structure of PMPS was characterized by FTIR spectra; the thermal properties of PMPS was characterized and analyzed by TGA. PMPSF was used in sheepskin wet blue leather and compared with oxidative sulfited vegetable oils (L-3) and RF-1 fatliquor; the waterproof and mechanical properties of the leather were measured. The results show that the thermal decomposition temperature of PMPS is 192.8°C. Fatliquor PMPSF provids a good level-dyeing property, the leather oiled by it has good flexibility and fullness, the waterproofness and mechanical properties of leather are also good. Moreover, compared with the leather fatliquored by L-3, the thickening rate of leather oiled by PMPSF reaches 13.7%.
28
Grimwood, Jane, Lynn Olinger, and Richard S. Stephens. "Expression of Chlamydia pneumoniaePolymorphic Membrane Protein Family Genes." Infection and Immunity 69, no. 4 (April 1, 2001): 2383–89. http://dx.doi.org/10.1128/iai.69.4.2383-2389.2001.
Full textAbstract:
ABSTRACT The genome of the obligate intracellular bacterium Chlamydia pneumoniae CWL029 encodes a family of 21 proteins with predicted outer membrane localization. These polymorphic membrane proteins (Pmps) are heterogeneous in both amino acid sequence and predicted size but are unified by the conserved amino acid motifs GGAI and FXXN repeated in the N-terminal half of each protein. Reverse transcriptase PCR analysis showed that all pmp genes are transcribed. To determine whether all proteins are expressed, specific antisera were generated by immunization with mutually exclusive synthetic peptides representing each of the 21 predicted Pmps. Each antiserum reacted with, and was typically immunospecific for, the corresponding peptide immunogen by enzyme-linked immunosorbent assay. Western blot analyses of purified elementary bodies showed that 11 of the 21 Pmps were detectable. Attempts to demonstrate by Sarykosyl fractionation that the Pmps were localized to the outer membrane revealed that several of the Pmps were unstable and readily degraded. Analyses of additionalC. pneumoniae strains showed that although some Pmps are conserved, others vary between strains, in both molecular weight and level of expression.
29
Bauml, Joshua, Coby Basal, and Jun J. Mao. "Treatment of Post-Mastectomy Pain Syndrome with Acupuncture: A Case Report." Acupuncture in Medicine 32, no. 2 (April 2014): 183–85. http://dx.doi.org/10.1136/acupmed-2013-010459.
Full textAbstract:
Post-mastectomy pain syndrome (PMPS) is a common and severe neuropathic pain syndrome arising after breast surgery. Since few effective allopathic treatments exist for PMPS, many patients may seek assistance from complementary and alternative medicine. Here, we report a case of a woman with severe and persistent PMPS who was successfully treated with acupuncture.
30
Qin, Fangmei, Shunhua Huang, Zhitao Li, Jieyu Ye, and Jing Sun. "The Platelet-Derived Microparticles Related to the Clinical Phenotype Heterogeneity of Hemophlia a." Blood 124, no. 21 (December 6, 2014): 2828. http://dx.doi.org/10.1182/blood.v124.21.2828.2828.
Full textAbstract:
Abstract Keywords: Hemophlia A, Phenotype; Heterogeneity; Flowcytometry ; Mircoparticle Background: The clinical phenotype for hemophlia A are typically recognized sub-clinical, mild, moderate and severe according to the residue FVIII level of the plasma. For recent years, there are evidences indicate that the residue FVIII level is not the only factor relate to the clinical phenotype of patients with severe hemophlia A. Platelets are an important role in hemostasis and platelet-derived microparticles (PMPs) are known to mediate a prothrombotic state in patients, we hypothesized that PMPs contributes to the clinical phenotype heterogeneity of hemophilia A patients. Methods: Thirty-one patients with severe hemophlia A (FVIII:C<1%, age 18-33 yrs, median 23.5 yrs) treated on demand were enrolled in this study, including 10 patients with mild type(FVIII:C<1%, but annualized bleed rates<6) and 21 patients with severe type (FVIII:C<1% and annualized bleed rates≥24). Seventeen healthy male donors were selected as normal controls. We also compared the baseline PMPs counts from eleven patients on demand treatment to that when they had three months prophylactic treatment. Venous blood was anticoagulated with sodium citrate (1:9). Plasma was isolated (1550g for 20 min at RT) and then cell free supernatant was ultracentrifuged (18,000g for 20 min at 20°C). The supernatant plasma samples were measured for baseline PMPs counts, with phycoerythrin (PE)-conjugated monoclonal antibodies CD41, CD42b, CD61, CD62P, and CD63 specific for PMPs derived from platelets respectively. Analyzed by flow cytometry, Forward scatter was set in scale using fluorescent microspheres of 1.0μm and standard fluorescent microbeads (0-1.0μm) in diameter were used to set the microparticle gate. Statistics were performed using independent-samples T test Results: There was a trend toward higher the mean level of PMPs counts in mild type patients compared to severe type (mean ± SD: 2.06±1.33 vs 0.95±0.64, p = 0.02), the PMPs counts of normal individuals was significantly higher than severe type(mean ± SD: 1.06±1.20 v s0.95±0.64, p = 0.02), significantly lower than mild type (mean ± SD: 1.06±1.20 vs 2.06±1.33, p = 0.02). The PMPs counts decreased after 3 months low dose (10 iu/kg, 2-3/w) prophylacticlly treatment (mean ± SD: 6.50±0.51 vs 0.45±0.50, p = 0.00). Conclusion/Discussion: Platelet-derived Microparticles (PMPs) are generated in response to platelet activation, our observations demonstrate that the PMPs counts differs in patients of severe hemophlia A, the mean lever of PMPs in mild clinical phenotype patients was higher than the severe type, prophylacticlly treated patients has a lower PMPs lever, PMPs is likely to play a role in clincal phenotype heterogeneity of sever hemophilia A. Disclosures No relevant conflicts of interest to declare.
31
Meijuan, Yang, Peng Zhiyou, Tang Yuwen, Feng Ying, and Chen Xinzhong. "A Retrospective Study of Postmastectomy Pain Syndrome: Incidence, Characteristics, Risk Factors, and Influence on Quality of Life." Scientific World Journal 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/159732.
Full textAbstract:
Objective. The underlying cause for postmastectomy pain syndrome (PMPS) and its impact on quality of life remain unclear. The objective of this study aims to determine retrospectively the prevalence of PMPS, its predicting risk factors, and its impact on quality of life.Method. In this survey, 225 women completed a battery of questionnaires. The questionnaires comprised the short form of the McGill Pain Questionnaire (SF-MPQ) exploring the characteristics and the description of the pain, and a Short Form-36 (SF-36) Health Survey evaluating quality of life. Logistic regression analyses were subsequently performed to identify risk factors for PMPS.Results. 62 women (27.6%) reported PMPS as a consequence of surgery, and the pain was generally mild, mostly localized in breast area and intermittent. The pain was mainly described as aching (62.9%). 144 women reported sensory disturbance. We found that only the younger age is the predictive factor for PMPS (P<0.05). Compared to the patients who did not experience PMPS, those who suffered from PMPS had significantly worse scores in role limitations due to physical problems (role physical, RP), body pain (BP), general health (GH), vitality (VT), role limitations due to emotional problems (role emotional, RE), and mental health (MH) (P<0.05).Conclusion. PMPS is a significant problem, and the possible risk factors should be further explored. Patients with PMPS have significant worse quality of life, suggesting that patients should be well informed about the likelihood of experiencing the pain, and they may be afforded greater predictability and higher perceived control to enhance their quality of life.
32
Pejenaute-Ochoa, María Dolores, Carlos Santana-Molina, Damien P. Devos, José Ignacio Ibeas, and Alfonso Fernández-Álvarez. "Structural, Evolutionary, and Functional Analysis of the Protein O-Mannosyltransferase Family in Pathogenic Fungi." Journal of Fungi 7, no. 5 (April 23, 2021): 328. http://dx.doi.org/10.3390/jof7050328.
Full textAbstract:
Protein O-mannosyltransferases (Pmts) comprise a group of proteins that add mannoses to substrate proteins at the endoplasmic reticulum. This post-translational modification is important for the faithful transfer of nascent glycoproteins throughout the secretory pathway. Most fungi genomes encode three O-mannosyltransferases, usually named Pmt1, Pmt2, and Pmt4. In pathogenic fungi, Pmts, especially Pmt4, are key factors for virulence. Although the importance of Pmts for fungal pathogenesis is well established in a wide range of pathogens, questions remain regarding certain features of Pmts. For example, why does the single deletion of each pmt gene have an asymmetrical impact on host colonization? Here, we analyse the origin of Pmts in fungi and review the most important phenotypes associated with Pmt mutants in pathogenic fungi. Hence, we highlight the enormous relevance of these glycotransferases for fungal pathogenic development.
33
Bei, Jun-Jie, Chuan Liu, Song Peng, Cheng-Hai Liu, Wei-Bo Zhao, Xiao-Long Qu, Qiang Chen, et al. "Staphylococcal SSL5-induced platelet microparticles provoke proinflammatory responses via the CD40/TRAF6/NFKB signalling pathway in monocytes." Thrombosis and Haemostasis 115, no. 03 (2016): 632–45. http://dx.doi.org/10.1160/th15-04-0322.
Full textAbstract:
SummaryPathogens-induced platelet activation contributes to inflammation in cardiovascular diseases, but underlying mechanisms remain elusive. Staphylococcal superantigen-like protein 5 (SSL5) is a known activator of platelets. Here we examined whether SSL5 is implicated in Staphylococcus aureus (S. aureus)-induced inflammation and potential mechanisms involved. As expected, we show that SSL5 activates human platelets and induces generation of platelet microparticles (PMPs). Flow cytometry and scanning electron microscopy studies demonstrate that SSL5-induced PMPs (SSL5-PMPs) bind to monocytes, causing aggregate formation. In addition, SSL5-PMPs provoke monocyte expression and release of inflammatory mediators, including interleukin-1β (IL-1β), tumour necrosis factor-α (TNFα), monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in a dose- and time-dependent manner. SSL5-PMPs also enhance MCP-1-induced monocyte migration. Blockade of CD40 and CD40 ligand (CD40L) interactions with neutralising antibodies significantly reduce monocyte release of inflammatory mediators and migration induced by SSL5-PMPs. SiRNA-mediated silencing of CD40 or TNF receptor (TNFR)-associated factor 6 (TRAF6) gene largely abrogates phosphorylation and nuclear translocation of NFkB (p65). In conclusion, SSL5 provokes the release of inflammatory mediators in monocytes, at least in part, via PMPs-mediated activation of the CD40/TRAF6/NFkB signalling pathway, though it normally inhibits leukocyte function. Our findings thus reveal a novel mechanism by which S. aureus induces inflammation.
34
Forlow, Stephen B., Rodger P. McEver, and Matthias U. Nollert. "Leukocyte-leukocyte interactions mediated by platelet microparticles under flow." Blood 95, no. 4 (February 15, 2000): 1317–23. http://dx.doi.org/10.1182/blood.v95.4.1317.004k30_1317_1323.
Full textAbstract:
Platelet microparticles (PMPs) are released from activated platelets and express functional adhesion receptors, including P-selectin, on their surface. PMP concentrations are elevated in many disorders, and their role in accelerating coagulation has been studied. However, their role in leukocyte aggregation has not been defined. We hypothesized that P-selectin–expressing PMPs bridge leukocytes that express P-selectin glycoprotein ligand-1 (PSGL-1), thereby allowing them to interact under flow conditions. PMPs were isolated from platelet-rich plasma or were generated by activating washed platelets with calcium ionophore. PMPs increased transient adhesion of flowing HL-60 cells or neutrophils to HL-60 cells or neutrophils prebound to the surface of a parallel plate flow chamber. Homotypic neutrophil interactions are initiated by the binding of L-selectin to PSGL-1. However, even when L-selectin function was blocked, PMPs allowed flowing neutrophils to aggregate and to interact with PSGL-1–expressing cells prebound to the surface of the flow chamber. The microparticle-mediated cell interactions occurred at lower shear stresses than those mediated by L-selectin. PMPs may enhance leukocyte aggregation and leukocyte accumulation on selectin-expressing substrates, especially in diseases where the concentration of the particles is elevated.
35
Barry, Orla, and Garret FitzGerald. "Mechanisms of Cellular Activation by Platelet Microparticles." Thrombosis and Haemostasis 82, no. 08 (1999): 794–800. http://dx.doi.org/10.1055/s-0037-1615913.
Full textAbstract:
IntroductionEukaryotic cells, after activation, shed components of their plasma membranes into the extracellular space.1,2 Such fragments may include cytoplasmic elements and are known colloquially as microparticles (MPs). Monocytes,3 lymphocytes,4 endothelial cells,5 erythrocytes,6 and granulocytes7 have been shown to vesiculate either in vitro or in vivo. MPs from other sources have also been reported to exist in vivo.8,9 In addition, platelets have been found to vesiculate following activation by agonists.10,11 Platelets activated with collagen and/or thrombin, by the Ca2+ ionophore A23187 or the complement protein C5b-9, induce platelet microparticle (PMP) formation. While the effect of these agonists is to increase platelet cytosolic Ca2+ concentration, it has been suggested that calpain activation,12-14 cytoskeletal reorganization,12,14,15 protein phosphorylation14 and phospholipid translocation16,17 also may have roles in PMP formation.Shear stress has been shown to induce platelet vesiculation. The mechanisms involved include the binding of von Willebrand factor (vWF) to either glycoprotein (GP)-Ib or GP IIb/IIIa.18 These in vitro observations are supported by an ex vivo model of high arterial shear stress. High shear stress, as pertains in the atherosclerotic vasculature, was shown to activate platelets and trigger PMP formation. Meanwhile, under physiological or simulated shear stress conditions in arteries with a minor degree of stenosis, no vesiculation occurred.19 Platelet microparticles contain surface receptors for both factor VIII, a cofactor in the tenase enzyme complex,20 and factor Va (which assembles with factor Xa to form the prothrombinase complex).10 While a transient expression of platelet membrane factor VIII binding has been reported, more stable factor VIII and factor Va has been reported for PMPs.20 High- and low-affinity binding sites for activated factor IX are also present on PMPs.21 Thus, PMPs have the potential to provide procoagulant activity at a distance from the site of platelet activation and for a longer period than activated platelets. Also, PMPs possess anticoagulant properties.22 These PMPs can bind protein S, an anticoagulant plasma protein responsible for degradation of the phospholipid-bound coagulation factor Va and factor VIII and which supports the binding of both protein C and activated protein C (APC). Coupled to the same platelet stimulation reactions, PMPs possess both pro- and anticoagulant properties. The relative distribution of pro- and anticoagulant activity between platelets and PMP remains approximately the same, irrespective of the agonist used, with approximately 25% of both activities associated with PMP. Furthermore, a recent study reported that protein C inhibitor, a member of the serpin family secreted from activated platelets, binds preferentially to the phosphatidylethanolamine (PE) of platelet membranes and PMPs, and efficiently inhibits phospholipid-bound APC.23 Similar to PMPs, MPs released from monocytes, lymphocytes, erythrocytes, and granulocytes demonstrate procoagulant activity, but whether they display anti-coagulant activity remains to be shown. The density of aminophospholipids also has been shown to be greater on PMPs than on remnant platelets.24,25 This may account for the preferential binding to PMPs over platelets of factor VIII,20 factor Va,10 and factor IXa.21 The PMP surface may provide the optimal phosphatidylserine (PS) level required by the binding sites of these blood clotting factors. Preferential binding of these critical factors favor the participation of PMPs in hemostatic protection and may explain the lack of bleeding symptoms in patients with autoimmune thrombocytopenia associated with high levels of PMPs.26 Elevated levels of PMPs in vivo have been reported for patients with activated coagulation and fibrinolysis,27 unstable angina,28 diabetes mellitus,29 sickle cell anemia,30 and human immunodeficiency virus (HIV).31 Recently, it was demonstrated for the first time that PMPs generated in vivo can stimulate coagulation.32 Procoagulant PMPs generated during coronary bypass surgery, especially in pericardial blood, supported coagulation via a tissue factor (TF)/ factor VII-dependent and factor XII-independent pathway.The functional importance of PMPs in human disease has not been well-defined. This is despite their pro- and anticoagulative properties10,16,20,22 and the convincing evidence that the PMP surface possesses the platelet—endothelium attachment receptors, glycoprotein GP IIb/IIIa, Ib, and IaIIa33-35 and P-selectin.34 Despite the association of PMPs with a range of clinical abnormalities,26-31,36 it remains unsolved whether persistent platelet activation, with concomitant formation of PMPs, is merely a consequence of the disease or reflects the influence of previously formed PMPs in the circulation.PMPs have become a popular focus of research, for both clinical and basic investigation. Recently, the possibility that MPs might, themselves, evoke cellular responses in the immediate microenvironment of their formation has been suggested. For example, endothelial cell activation by thrombin results in vesicle shedding, which, in turn, activates neutrophils and enhances their propensity to adhere to endothelial cells.37 Similarly, MPs shed from platelets activated with Staphylococcus aureus α-toxin induce platelet aggregation.38 The role of PMPs in modulating their local environment is the subject of this review. An overview of the mechanism(s) of cellular activation by PMPs will be provided. PMP-induced activation of platelets, human umbilical vein endothelial cells (HUVECs), monocytes, and U-937 (human promonocytic leukemia) cells have been used as models for assessing the possible biological effects of PMPs in vivo.
36
Manohar, Murli, Prem P. Sharma, and Dukjoon Kim. "Intercalated Poly (2-acrylamido-2-methyl-1-propanesulfonic Acid) into Sulfonated Poly (1,4-phenylene ether-ether-sulfone) Based Proton Exchange Membrane: Improved Ionic Conductivity." Molecules 26, no. 1 (December 31, 2020): 161. http://dx.doi.org/10.3390/molecules26010161.
Full textAbstract:
A series of hybrid proton exchange membranes were synthesized via in situ polymerization of poly (2-acrylamido-2-methyl-1-propanesulfonic acid) PMPS with sulfonated poly (1,4-phenylene ether-ether-sulfone) (SPEES). The insertion of poly (2-acrylamido-2-methyl-1-propanesulfonic acid) PMPS, between the rigid skeleton of SPEES plays a reinforcing role to enhance the ionic conductivity. The synthesized polymer was chemically characterized by fourier-transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance 1H NMR spectroscopy to demonstrate the successful grafting of PMPS with the pendent polymer chain of SPEES. A variety of physicochemical properties were also investigated such as ion exchange capacity (IEC), proton conductivity, water uptake and swelling ratio to characterize the suitability of the formed polymer for various electrochemical applications. SP-PMPS-03, having the highest concentration of all PMPS, shows excellent proton conductivity of 0.089 S cm−1 at 80 °C which is much higher than SPEES which is ~0.049 S cm−1. Optimum water uptake and swelling ratio with high conductivity is mainly attributed to a less ordered arrangement polymer chain with high density of the functional group to facilitate ionic transport. The residual weight was 93.35, 92.44 and 89.56%, for SP-PMPS-01, 02 and 03, respectively, in tests with Fenton’s reagent after 24 h. In support of all above properties a good chemical and thermal stability was also achieved by SP-PMPS-03, owing to the durability for electrochemical application.
37
Kireev, Dmitry, Nadezhda Popenko, Aleksei Pichugin, Mikhail Panteleev, Olga Krymskaya, Fazoil Ataullakhanov, and Elena Sinauridze. "Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets." Thrombosis and Haemostasis 97, no. 03 (2007): 425–34. http://dx.doi.org/10.1160/th06-06-0313.
Full textAbstract:
SummaryPlatelet microparticles (PMPs) are small vesicles released from blood platelets upon activation. The procoagulant activity of PMPs has been previously mainly characterized by theirability to bind coagulation factors VIII and Va in reconstructed systems. It can be supposed that PMPs can contribute to the development of thrombotic complications in the pathologic states associated with the increase of their blood concentration. In this study we compared procoagulant properties of calcium ionophore A23187-activated platelets and PMPs using several in-vitro models of hemostasis. Surface densities of phosphatidylserine, CD61, CD62P and factor X bound per surface area unit were determined by flow cytometry. They were 2.7-, 8.4-, 4.3-, and 13-fold higher for PMPs than for activated platelets, respectively. Spatial clot growth rate (Vclot) in the reaction-diffus ion experimental model and endogenous thrombin potential (ETP) were determined in plasma, which was depleted of phospholipid cell surfaces by ultra-centrifugation and supplemented with activated platelets or PMPs at different concentrations. Both Vcllot and ETP rapidly increased with the increase of PMP or platelet concentration until saturation was reached. The plateau values of Vclot and ETP for activated platelets and PMPs were similar. In both assays, the procoagulant activity of one PMP was almost equal to that of one activated platelet despite at least two-orders-of-magnitude difference in their surface areas. This suggests that the PMP surface is approximately 50- to 100-fold more procoagulant than the surface of activated platelets.
38
Britting, A., W. Eyrich, A. Lehmann, and F. Uhlig. "Lifetime-issues of MCP-PMTs." Journal of Instrumentation 6, no. 10 (October 5, 2011): C10001. http://dx.doi.org/10.1088/1748-0221/6/10/c10001.
Full text
39
Rottensteiner, Hanspeter, Achim Kramer, Stephan Lorenzen, Katharina Stein, Christiane Landgraf, Rudolf Volkmer-Engert, and Ralf Erdmann. "Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals." Molecular Biology of the Cell 15, no. 7 (July 2004): 3406–17. http://dx.doi.org/10.1091/mbc.e04-03-0188.
Full textAbstract:
Targeting of peroxisomal membrane proteins (PMPs) is a multistep process that requires not only recognition of PMPs in the cytosol but also their insertion into the peroxisomal membrane. As a consequence, targeting signals of PMPs (mPTS) are rather complex. A candidate protein for the PMP recognition event is Pex19p, which interacts with most PMPs. However, the respective Pex19p-binding sites are ill-defined and it is currently disputed whether these sites are contained within mPTS. By using synthetic peptide scans and yeast two-hybrid analyses, we determined and characterized Pex19p-binding sites in Pex11p and Pex13p, two PMPs from Saccharomyces cerevisiae. The sites turned out to be composed of a short helical motif with a minimal length of 11 amino acids. With the acquired data, it proved possible to predict and experimentally verify Pex19p-binding sites in several other PMPs by applying a pattern search and a prediction matrix. A peroxisomally targeted Pex13p fragment became mislocalized to the endoplasmic reticulum in the absence of its Pex19p-binding site. By adding the heterologous binding site of Pex11p, peroxisomal targeting of the Pex13p fragment was restored. We conclude that Pex19p-binding sites are well-defined entities that represent an essential part of the mPTS.
40
Cacic, Daniel, Oddmund Nordgård, Peter Meyer, and Tor Hervig. "Platelet Microparticles Decrease Daunorubicin-Induced DNA Damage and Modulate Intrinsic Apoptosis in THP-1 Cells." International Journal of Molecular Sciences 22, no. 14 (July 6, 2021): 7264. http://dx.doi.org/10.3390/ijms22147264.
Full textAbstract:
Platelets can modulate cancer through budding of platelet microparticles (PMPs) that can transfer a plethora of bioactive molecules to cancer cells upon internalization. In acute myelogenous leukemia (AML) this can induce chemoresistance, partially through a decrease in cell activity. Here we investigated if the internalization of PMPs protected the monocytic AML cell line, THP-1, from apoptosis by decreasing the initial cellular damage inflicted by treatment with daunorubicin, or via direct modulation of the apoptotic response. We examined whether PMPs could protect against apoptosis after treatment with a selection of inducers, primarily associated with either the intrinsic or the extrinsic apoptotic pathway, and protection was restricted to the agents targeting intrinsic apoptosis. Furthermore, levels of daunorubicin-induced DNA damage, assessed by measuring gH2AX, were reduced in both 2N and 4N cells after PMP co-incubation. Measuring different BCL2-family proteins before and after treatment with daunorubicin revealed that PMPs downregulated the pro-apoptotic PUMA protein. Thus, our findings indicated that PMPs may protect AML cells against apoptosis by reducing DNA damage both dependent and independent of cell cycle phase, and via direct modulation of the intrinsic apoptotic pathway by downregulating PUMA. These findings further support the clinical relevance of platelets and PMPs in AML.
41
Koiou, Ekaterini, Konstantinos Tziomalos, Ilias Katsikis, Emmanuil Kalaitzakis, Eleni A. Kandaraki, Elena A. Tsourdi, Dimitrios Delkos, Efstathios Papadakis, and Dimitrios Panidis. "Circulating platelet-derived microparticles are elevated in women with polycystic ovary syndrome diagnosed with the 1990 criteria and correlate with serum testosterone levels." European Journal of Endocrinology 165, no. 1 (July 2011): 63–68. http://dx.doi.org/10.1530/eje-11-0144.
Full textAbstract:
ObjectiveWomen with polycystic ovary syndrome (PCOS) appear to have higher cardiovascular risk than healthy population. Patients diagnosed with PCOS according to the 1990 criteria have a more adverse metabolic profile than those diagnosed with the 2003 criteria. Platelet-derived microparticles (PMPs) appear to contribute to atherosclerosis but have not been assessed in PCOS. The aim of this study was to determine plasma PMPs in PCOS patients.DesignA cross-sectional study.MethodsWe assessed plasma PMPs in 76 normal weight women with PCOS (39 belonging to the phenotypes 1 and 2 (group I) and 37 belonging to the phenotypes 3 and 4 (group II)) and 21 healthy normal weight women.ResultsMarkers of obesity and insulin resistance did not differ between women with PCOS and controls. Serum testosterone levels and the free androgen index (FAI) were higher in group I than in group II and controls (P<0.001 for all comparisons) but did not differ between the latter two groups. Plasma PMPs were higher in group I than in controls (P=0.018) but did not differ between group II and controls or between groups I and II. In the total study population (n=97), plasma PMPs correlated with serum testosterone levels (r=0.207, P=0.042) and the FAI (r=0.207, P=0.042).ConclusionsPlasma PMPs are elevated in women with phenotypes 1 and 2 of PCOS compared with healthy controls, but not in women with phenotypes 3 and 4. Hyperandrogenemia, which is more pronounced in phenotypes 1 and 2, appears to be implicated in the increase in plasma PMPs.
42
Zahran, Asmaa M., Ismail L. Mohamed, Osama M. El Asheer, Deiaaeldin M. Tamer, Mohamed G. M. Abo-ELela, Mona H. Abdel-Rahim, Omnia H. B. El-Badawy, and Khalid I. Elsayh. "Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus." Clinical and Applied Thrombosis/Hemostasis 25 (January 1, 2019): 107602961882531. http://dx.doi.org/10.1177/1076029618825311.
Full textAbstract:
Background and Aim: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulating endothelial cells (CECs), endothelial and platelet derived micropaticles (EMPs, PMPs) and total microparticles (TMPs), in T1D children in relation to each other and to the metabolic disorders accompanying T1D. Patients and Methods: Thirty T1D patients and 20 age and sex matched healthy volunteers were assessed for HbA1c level and lipid profile. Quantification of CECs, EPCs, TMPs, EMPs and PMPs was done by flow cytometry. Results: The mean levels of EMPs, PMPs, TMPs and CECs were significantly higher in diabetic children compared to controls. Meanwhile, the levels of EPCs were significantly lower in diabetic children compared to controls. Both PMPs and CECs showed the highest significant differences between patients and controls and their levels were directly related to HbA1c, total cholesterol, LDL and triglycerides. A moderate correlation was observed between the frequency of PMPs and CECs. EPCs revealed negative correlations with both LDL and triglycerides. TMPs were only related to LDL, while EMPs were only related to HbA1c. Conclusion: Although there is disturbance in the levels of EMPs, PMPs, TMPs, CECs and EPCs in type 1 diabetic children compared to the controls, only the levels of PMPs and CECs were closely affected by the poor glycemic control and dyslipidemia occurring in T1D; thus may contribute to a higher risk of cardiovascular diseases.
43
Qiang, Tao Tao, Xiang Luo, Long Fang Ren, Xue Chuan Wang, and Bao Yuan He. "Preparation, Characterization of Silicone Succinate Ester and its Application in Leather Industry." Advanced Materials Research 197-198 (February 2011): 409–16. http://dx.doi.org/10.4028/www.scientific.net/amr.197-198.409.
Full textAbstract:
The silicone succinate ester surfactant (PMPS) was synthesized by the raw materials of polyether alcohol amine modified polysilosane (PAPS) and maleic anhydride (MA) at 90°C for 4.5h, and p-toluenesulfonic acid (PTSA) was used as the catalyst. Sulfated neatsfoot oil, oxidized-sulfited fish oil was mixed with PMPS to prepare a new leather fatliqour (F-1). The structure of the materials and PMPS were characterized by FTIR. The biodegradability of F-1 was analyzed by BOD5 and CODCr and the tissue slices of the leather samples oiled by PMPS were observed by Multimedia Microscope to study the fatliquoring effect. The waterproof properties and the fatliquored leather were determined. All the results indicated that the F-1 fatliquor has good fatliquoring effect.
44
Wei, Yanze, Jiming Chen, Yaoheng Zhang, and Zaijun Lu. "Color changing Pickering emulsions stabilized by polysiloxane microspheres bearing phenolphthalein groups." RSC Advances 5, no. 88 (2015): 71824–29. http://dx.doi.org/10.1039/c5ra12453k.
Full textAbstract:
PMPs were synthesized via benzoxazine chemistry. Toluene-in-water Pickering emulsions stabilized by PMPs exhibit color changing behavior and two emulsification/demulsification processes occurred at pH 9 and 12, respectively.
45
Ifelebuegu, Augustine, Habibath Salauh, Yihuai Zhang, and Daniel Lynch. "Adsorptive Properties of Poly(1-methylpyrrol-2-ylsquaraine) Particles for the Removal of Endocrine-Disrupting Chemicals from Aqueous Solutions: Batch and Fixed-Bed Column Studies." Processes 6, no. 9 (September 4, 2018): 155. http://dx.doi.org/10.3390/pr6090155.
Full textAbstract:
The adsorptive properties of poly(1-methylpyrrol-2-ylsquaraine) (PMPS) particles were investigated in batch and column adsorption experiments as alternative adsorbent for the treatment of endocrine-disrupting chemicals in water. The PMPS particles were synthesised by condensing 3,4-dihydroxycyclobut-3-ene-1,2-dione (squaric acid) with 1-methylpyrrole in butanol. The results demonstrated that PMPS particles are effective in the removal of endocrine disrupting chemicals (EDCs) in water with adsorption being more favourable at an acidic pH, and a superior sorption capacity being achieved at pH 4. The results also showed that the removal of EDCs by the PMPS particles was a complex process involving multiple rate-limiting steps and physicochemical interactions between the EDCs and the particles. Gibbs free energy of −8.32 kJ/mole and −6.6 kJ/mol, and enthalpies of 68 kJ/mol and 43 kJ/mol, were achieved for the adsorption E2 and EE2 respectively The removal efficiencies of the EDCs by PMPS particles were comparable to those of activated carbon, and hence can be applied as an alternative adsorbent in water treatment applications.
46
van der Zee, P. Marc, Éva Biró, Yung Ko, Robbert J. de Winter, C. Erik Hack, Augueste Sturk, and Rienk Nieuwland. "P-Selectin- and CD63-Exposing Platelet Microparticles Reflect Platelet Activation in Peripheral Arterial Disease and Myocardial Infarction." Clinical Chemistry 52, no. 4 (April 1, 2006): 657–64. http://dx.doi.org/10.1373/clinchem.2005.057414.
Full textAbstract:
Abstract Background: Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. We studied the extent to which PMP subpopulations parallel platelet activation in vitro and in vivo. Methods: Using flow cytometry, we analyzed PMP subpopulations from resting and activated platelets in vitro (n = 6) as well as from plasma samples of patients with stable angina, peripheral arterial disease, or myocardial infarction [non-ST-elevation (NSTEMI) and ST-elevation (STEMI)] and from older, age- and sex-matched and young healthy individuals [n = 10 for all groups except NSTEMI (n = 11)]. Coagulation markers prothrombin fragment F1 + 2 and thrombin-antithrombin complexes were determined by ELISA. The PMP-associated fraction of soluble (s)P-selectin was estimated by ELISA. Results: In vitro, stimulation of platelets with thrombin receptor–activating peptide (15 μmol/L) or the calcium ionophore A23187 (2.5 μmol/L) increased fractions of both platelets and PMPs exposing P-selectin or CD63 (P <0.001 for all). Whereas the number of PMPs released by A23187-stimulated platelets increased significantly (P <0.001), the number of PMPs released from thrombin receptor-activating peptide–stimulated platelets remained constant (P >0.05). Ex vivo, numbers of circulating PMPs were comparable in all groups. Compared with young persons, P-selectin–exposing PMPs were increased in older persons (P = 0.02) and were further increased in patients with NSTEMI (P = 0.007) and STEMI (P = 0.045). CD63-exposing PMPs were increased in patients with peripheral arterial disease (P = 0.041), NSTEMI (P = 0.001), and STEMI (P = 0.049). Subpopulations exposing P-selectin or CD63 correlated with each other (r = 0.581; P <0.001), but neither correlated with the plasma concentrations of F1 + 2 or thrombin–antithrombin complexes. The PMP-associated fraction of sP-selectin constituted only 2.2 (4.7)% [mean (SD)] of total sP-selectin. Conclusions: PMP subpopulations reflect platelet activation status better than the total number of PMPs. Increased concentrations of circulating PMP subpopulations are found in aging, and further increases are encountered in peripheral arterial disease and myocardial infarction.
47
Gladović, Pavle, Vladimir Popović, and Velibor Peulić. "Expenditure Model of Line Ranking in the Public Mass Passengers Transportation System." PROMET - Traffic&Transportation 23, no. 6 (February 21, 2012): 503–9. http://dx.doi.org/10.7307/ptt.v23i6.185.
Full textAbstract:
In all the cities of Serbia that have organized systems of public mass passenger transport (PMPT), over the past several years more and more private transport companies are being included in the network of city and suburban transportation lines. The inclusion of private transport companies in the PMPT system via public tenders should be preceded by the procedure of calculation of the realistic income and system functioning expenses in order to establish possible balances of needs for City Budget subsidies, as well as the elements on Contract of entrusting this vital communal activity of every city. The new principle for determining the total cost of PMPT system functioning, based on line difficulty ranking, has been presented in this paper. KEY WORDS: expenditure model, price, PMPT system, line, exploitation parameters, evaluation, line rank
48
Shapkin, Nikolai P., L. B. Leontiev, I. G. Khal'chenko, and V. N. Makarov. "The Influence of Physicochemical and Structural Properties of Polymetallorganosiloxanes on their Tribotechnical Characteristics." Materials Science Forum 992 (May 2020): 724–32. http://dx.doi.org/10.4028/www.scientific.net/msf.992.724.
Full textAbstract:
The effect of molecular structure and physicochemical parameters of polymetalphenylsiloxanes in a solid state on their tribotechnical properties has been investigated. Characteristics of polymetalphenylsiloxanes (PMPS) have been determined by means of physicochemical methods. Inner structure of a solid phase of PMPS has been examined by means of positron annihilation spectroscopy (PAS). Minimal sizes of ordered (matrix) and disordered (voids) regions were calculated based on the obtained PAS data. Specific polarizing potentials responsible for the stability of PMPS spherical particles have been calculated based on physicochemical parameters.
49
Matsumoto, Yoshihiro, Shingo Baba, Makoto Endo, Nokitaka Setsu, Keiichiro Iida, Jun-Ichi Fukushi, Kenichi Kawaguchi, et al. "Metabolic Tumor Volume by 18F-FDG PET/CT Can Predict the Clinical Outcome of Primary Malignant Spine/Spinal Tumors." BioMed Research International 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/8132676.
Full textAbstract:
Background and Purpose. Primary malignant spine/spinal tumors (PMSTs) are rare and life-threatening diseases. In this study, we demonstrated the advantage of volume-based 18F-FDG PET/CT metabolic parameter, metabolic tumor volume (MTV), for assessing the aggressiveness of PMSTs. Materials and Methods. We retrospectively reviewed 27 patients with PMSTs and calculated SUVmax, MTV, and total lesion glycolysis (TLG) to compare their accuracy in predicting progression-free survival (PFS) and overall survival (OS) by receiver operating characteristic (ROC) curve analysis. Univariate and multivariate analyses were used to compare the reliability of the metabolic parameters and various clinical factors. Results. MTV exhibited greater accuracy than SUVmax or TLG. The cut-off values for PFS and OS derived from the AUC data were MTV 45 ml and 83 ml and TLG 250 SUV⁎ml and 257 SUV⁎ml, respectively. MTV above cut-off value, but not TLG, was identified as significant prognostic factor for PFS by log-lank test (p=0.04). In addition, MTV was the only significant predictive factors for PFS and OS in the multivariate analysis. Conclusions. MTV was a more accurate predictor of PFS and OS in PMSTs compared to TLG or SUVmax and helped decision-making for guiding rational treatment options.
50
Ashfaq Ahmad, Muhammad Bilal, Khalid Latif, and Zainab. "A Study of Project Management Processes for Sustainable and Successful Projects in Software Industry: Expectations vs Perceptions of Managers." Journal of Accounting and Finance in Emerging Economies 7, no. 1 (January 26, 2021): 103–15. http://dx.doi.org/10.26710/jafee.v7i1.1569.
Full textAbstract:
Project Management Processes (PMPs) are essential to avoid project failures due to the complexity and nature of projects in the software industry, particularly in emerging economies. The software industry is growing rapidly in Pakistan with an increasing number of local, regional and international clients. The project managers who are familiar with PMPs are therefore needed for the proper implementation of these processes, which will lead to sustainable and successful software projects. However, very limited studies have analyzed the expectations and perceptions of the project managers of these PMPs. In order to fill this gap, therefore, this study examined the role of PMPs in the sustainable development and success of software projects by documenting the expectations and perceptions of managers. A structured questionnaire is designed to collect data from 143 participants working in software houses. SPSS is used for the processing and analysis of data using selected statistical tools. The results show a clear difference in expectations and perceptions for PMPs, which means that project managers are of a less rational, sentimental and emotional nature. The findings of this study also show that the male segment is dominant in the software industry which may be due to of Pakistan’s specific social and cultural environment. There is however no significant difference between expectations and perceptions of both male and female project managers for PMPs. The findings of this study will help researchers, practitioners, academics and other stakeholders in the software industry.