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Dissertations / Theses on the topic 'Pneumatically assisted desorption/ionization'

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Published: 11 March 2023

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1

ZANCANARO, Flavio. "Mass spectrometry pneumatically assisted desorption/ionization in forensic toxicology." Doctoral thesis, Università degli Studi di Verona, 2010. http://hdl.handle.net/11562/342863.

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La spettrometria di massa è una delle tecniche più rilevanti in tossicologia clinica e forense. Il suo sviluppo e il miglioramento si basano sull'invenzione e l'uso di nuove sorgenti di ioni, nuovi metodi di ionizzazione, nuovi analizzatori di massa e nuove tecniche di pre-trattamento dei campioni. Una recente innovazione è la capacità di registrare spettri di massa su campioni reali direttamente nel loro ambiente nativo, senza preparazione del campione o pre-separazione. In questo ambito è stato descritto un nuovo metodo di ionizzazione/desorbimento chiamato DESI (desorbimento Electrospray io

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2

Peng, Ivory Xingyu. "Electrospray-assisted laser desorption ionization mass spectrometry for proteomic studies." Diss., Restricted to subscribing institutions, 2009. http://proquest.umi.com/pqdweb?did=1997571271&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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3

Berhane, Beniam T. "Rapid Characterization of Posttranscriptional Modifications in RNA Using Matrix Assisted Laser Desorption Ionization Mass Spectrometry and Matrix Assisted Laser Desorption Ionization Post Source Decay Mass Spectrometry." University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1052319621.

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4

Dubois, Frédéric Dubois Frédéric. "Ion formation and detection in matrix-assisted laser desorption/ionization mass spectrometry /." [S.l.] : [s.n.], 1999. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=13255.

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5

Dai, Yuqin. "Development of matrix-assisted laser desorption ionization mass spectrometry for biopolymer analysis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0003/NQ39519.pdf.

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6

Tummala, Manorama. "Surfactant-Aided Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (SA-MALDI MS)." University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1100672049.

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7

Dashtiev, Maxim. "Fluorescence spectroscopy of trapped molecular ions produced with matrix-assisted laser desorption/ionization /." Zürich : ETH, 2006. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=16949.

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8

Segu, Mohideen Mohamed Zaneer. "TARGET MODIFICATION FOR ENHANCED PERFORMANCE MATRIX ASSISTED LASER DESORPTION IONIZATION (MALDI) MASS SPECTROMETRY." Available to subscribers only, 2008. http://proquest.umi.com/pqdweb?did=1674093101&sid=1&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Thesis (Ph. D.)--Southern Illinois University Carbondale, 2008.<br>"Department of Chemistry." Keywords: Enhanced MALDI, MALDI-MS, On-probe separation, Protein-surface interactions, Sublayers, Surface binding capacity. Includes bibliographical references (p. 130-148). Also available online.

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9

Akinapalli, Srikanth. "MICROFLUIDIC DYNAMIC ISOELECTRIC FOCUSING COUPLED TO MATRIX ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY." OpenSIUC, 2016. https://opensiuc.lib.siu.edu/dissertations/1289.

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Proteomics is an increasingly important area of biological research and has gathered much attention over recent years. Major challenges that make a proteomic analysis difficult are sample complexity, diversity and dynamic range. Progress in the area of proteomics relies heavily on new analytical tools for the sensitive, selective, and high-throughput studies of target analytes. It is estimated that there are several hundred thousand proteins in a human cell. In order to be able to analyze such a complex sample, an analytical method must be capable of separating and detecting many differen

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10

Xiang, Fan. "Improvement and investigation of sample preparation for matrix-assisted laser desorption/ionization of proteins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25780.pdf.

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11

Wang, Jian. "Analyses of food and feed compounds using matrix-assisted laser desorption/ionization mass spectrometry." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0013/NQ59690.pdf.

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12

Köchling, Heinrich J. (Heinrich Josef) 1962. "Advancements in matrix-assisted laser desorption ionization mass spectrometry of peptides, proteins and polymers." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/10020.

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13

Mowat, Ian A. "Synthetic polymer analysis using matrix assisted laser desorption/ionization time-of-flight mass spectrometry." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/12128.

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The aim of the work described in this thesis was to assess 'Matrix Assisted Laser Desorption/Ionization' (MALDI) Time-of-Flight mass spectrometry as a possible technique for the analysis of synthetic polymers. A compact home-built time-of-flight mass spectrometer of cylindrically symmetrical geometry was used to carry out all the mass spectrometry detailed described in this thesis. A survey of the literature describing the development of matrix assisted laser desorption/ionization spectrometry and also the previous analysis of polymers by laser mass spectrometry was carried out. Experiments co

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14

Mazarin, Michaël. "L'ionisation MALDI [Matrix assisted laser desorption/ionization] de polymères synthétiques en spectrométrie de masse." Aix-Marseille 1, 2008. http://theses.univ-amu.fr.lama.univ-amu.fr/2008AIX11034.pdf.

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La spectrométrie de masse après ionisation/désorption laser assistée par matrice (MALDI) apparaît comme une technique de choix pour la caractérisation structurale des polymères synthétiques. Néanmoins, parce que les processus fondamentaux qui régissent la technique MALDI sont encore mal connus, l’optimisation des conditions expérimentales reste empirique. L’objectif de ces travaux de thèse est d’utiliser conjointement des techniques de spectrométrie de masse et de résonance magnétique nucléaire (RMN) pour rationaliser les méthodologies d'analyse des polymères synthétiques par MALDI. Une premiè

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15

Garrett, Timothy J. "Imaging small molecules in tissue by matrix-assisted laser desorption/ionization tandem mass spectrometry." [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0013807.

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16

Priyasantha, Kandalama KD. "DEVELOPMENT OF A NOVEL MATRIX ASSISTED LASER DESORPTION / IONIZATION (MALDI) BASED PEPTIDE QUANTITATION APPROACH." OpenSIUC, 2015. https://opensiuc.lib.siu.edu/dissertations/989.

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Matrix Assisted Laser Desorption / Ionization (MALDI) Mass Spectrometry (MS) has emerged as an important tool in the field of proteomics mainly because it is simple, quick and efficient. The identification and quantitation of biomarkers, protein targets for drugs, and metabolites are some of the important fields in proteomics research. Although MALDI MS is an important tool in proteomics research there are drawbacks of the technique that need further development in order for the approach to be used in clinical laboratories. One major limitation of MALDI MS is the generally poor reproducibility

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17

Astorga-Wells, Juan. "Microfluidic electrocapture technology in protein and peptide analysis /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-965-x/.

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18

Erb, William Joseph Owens Kevin G. "Exploration of the fundamentals of matrix assisted laser desorption/ionization time-of- flight mass spectrometry /." Philadelphia, Pa. : Drexel University, 2007. http://hdl.handle.net/1860/1765.

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19

Tang, Ho-wai, and 鄧浩維. "Studies on surface-assisted laser desorption/ionization and its analytical application in imaging mass spectrometry." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47145559.

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Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) is an analytical technique enabling direct chemical analysis of solid samples. Analytes could be desorbed/ionized upon nitrogen laser irradiation from a SALDI substrate-coated sample, then analyzed by MS. The substrate is involved in the transfer of laser energy to the analytes, and eventually assists the desorption/ionization of analytes. The analytical performance of SALDI-MS, such as detection sensitivity, is dependent on different parameters of the substrate, such as size, morphology and form. In this thesis, the ef

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20

Smith, Donna M. "Matrix Assisted Laser Desorption Ionization Quadrupole Time-of-Flight Mass Spectrometry of Poly(2-Vinylpyridine)." University of Akron / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=akron1110337309.

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21

Fiorentino, Michael Armond. "Immediate observation of matrix assisted laser desorption ionization products in a Fourier transform mass spectrometer /." Full text (PDF) from UMI/Dissertation Abstracts International, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p3004264.

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22

Moyer, Susanne Catharine. "Phosphotyrosine peptide fragmentation by atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry." Available to US Hopkins community, 2002. http://wwwlib.umi.com/dissertations/dlnow/3080729.

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23

An, Hyun Joo. "Characterization of protein-bound oligosaccharides using matrix-assisted laser desorption/ionization Fourier transform mass spectrometry /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.

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24

Ochoa, Mariela L. "Forensic and Proteomic Applications of Thermal Desorption Ion Mobility Spectrometry and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry." Ohio University / OhioLINK, 2005. http://www.ohiolink.edu/etd/view.cgi?ohiou1113585811.

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25

Krutchinsky, Andrew. "A collisional damping interface for a time-of-flight mass spectrometer with both electrospray ionization and matrix assisted laser desorption ionization." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape15/PQDD_0009/NQ31999.pdf.

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26

Chen, Yanfeng. "Analysis of Biological Molecules Using Stimulated Desorption Photoionization Mass Spectrometry." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/14620.

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Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) is a novel technique for direct analysis of organic and biological molecules. Amino acids, dipeptides, and organoselenium compounds were successfully detected by SALDI on carbon and silicon surfaces. Surface effects, solvent effects, temperature effects and pH effects were studied. A possible mechanism of SALDI is proposed based on observed results. In general, stimulated desorption results in neutral yields that are much larger than ion yields. Thus, we have exploited and further developed laser desorption single

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27

Zhang, Jinhua. "Characterization and structural elucidation of oligosaccharides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.

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28

SUH, MOO-JIN. "MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY OF BACTERIAL RIBOSOMAL PROTEINS AND RIBOSOMES." University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1106583486.

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29

Abell, Darcy Cameron. "Analysis of potato glycoalkaloids by ELISA and matrix assisted laser desorption/ionization time-of-flight mass spectroscopy /." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq22940.pdf.

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30

Ratcliffe, Lucy Vivien. "Proteomic strategies for protein and biomarker identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)." Thesis, Nottingham Trent University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431885.

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This thesis describes the development of novel strategies for the analysis of peptides by MALDI mass spectrometry. The developed techniques are applied to the identification of protein and proteomic biomarkers for melanoma. A commercial atmospheric pressure (APMALDI) source (MassTechnologies, Burtonsville, MD, USA) was modified to allow operation with a high powered nitrogen laser and independent PC control of the sample stage. A software interface was developed using LabVIEW 6.1 that allows full control of the target position with respect to the laser fibre optic interface, allowing the targe

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31

Eng, Tony Liang. "De novo peptide sequencing from matrix-assisted laser desorption/ionization-time of flight post-source-decay spectra." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/86584.

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32

Hoteling, Andrew J. "MALD/I TOF PSD and CID : understanding precision, resolution, and mass accuracy and MALD/I TOFMS : investigation of discrimination issues related to solubility /." Philadelphia, Pa. : Drexel University, 2004. http://dspace.library.drexel.edu/handle/1860/318.

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33

Holcomb, April M. Owens Kevin G. "Investigation into the ionization mechanism occurring in matrix assisted laser desorption ionization and factors affecting ion flight time in MALDI time-of-flight mass spectrometry /." Philadelphia, Pa. : Drexel University, 2009. http://hdl.handle.net/1860/3161.

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34

Sorensen, Christina M. "ESI-MS and MALDI-TOF-MS for the characterization and analysis of metallo-oligomers and proteins." Laramie, Wyo. : University of Wyoming, 2005. http://proquest.umi.com/pqdweb?did=1031044031&sid=4&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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35

Driedger, Darcy R. "Analysis of potato glycoalkaloids by immunoassay coupled to capillary electrophoresis or matrix-assisted laser desorption/ionization mass spectrometry." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0011/NQ59948.pdf.

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36

Lam, Sze-ki Clare, and 林思琪. "Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification and antifungal susceptibility of penicillium marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206605.

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Penicillium marneffei is the most important thermal dimorphic fungus causing systemic mycosis in HIV-infected patients in Southeast Asia. However, laboratory diagnosis based on microscopic morphology and mycelial-to-yeast conversion is time-consuming and expertise-dependent. The performance of the Bruker MALDI-TOF MS system for identification of mold and yeast cultures of 59 P. marneffei strains were evaluated by using the direct transfer method. Using the Bruker databases, BDAL v4.0.0.1 and Filamentous Fungi Library 1.0, the 59 P. marneffei strains grown in mold and yeast phase were identifie

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37

Dunn, Jamie D. "Polymer-modified plates for enrichment of phosphopeptides prior to analysis by matrix-assisted laser desorption/ionization mass spectrometry." Diss., Connect to online resource - MSU authorized users, 2007.

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38

Chen, Cheng-Tai, and 陳振泰. "Study of TiO2-sol-gel assisted desorption/ionization mass spectrometry." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/11145021475112867836.

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碩士<br>國立交通大學<br>應用化學系碩博士班<br>91<br>Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been widely used in the analysis for various types of analytes. The analysis results mainly rely on the selections of MALDI matrices. The solubility and co-crystallization between analytes and matrices are the major concerns in MALDI analysis. A new matrix system was developed to replace the usage of conventional MALDI matrix to avoid these problems. The TiO2 sol-gel-deposited thin film was employed as the sample substrate to assist the UV laser desorption/ionization of analytes in l

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39

Chiang, Ni-Chen, and 江妮蓁. "Nanomaterials for Bioanalysis in Surface-Assisted Laser Desorption/Ionization Mass Spectrometry." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/72763000084814143996.

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碩士<br>國立臺灣大學<br>化學研究所<br>97<br>This thesis is divided into two parts. In the first part, we have employed two sets of differently sized (average diameters: 3.5 and 14 nm) gold nanoparticles (Au NPs) as selective probes and matrices for the determination of aminothiols in biological samples using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Because of their higher ionization efficiency, the 3.5-nm-diameter Au NPs exhibited greater desorption/ionization efficiency toward the aminothiols than did the larger Au NPs; centrifugation of these small Au NPs was, however,

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40

Wang, Yu-Wen, and 王鈺雯. "Development of Electrode-Assisted Desorption Electrospray Ionization and Its biomedical Applications." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/67738758668009086608.

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碩士<br>臺灣大學<br>化學研究所<br>98<br>Ambient ionization mass spectrometry has recently played an important role in Biological Mass Spectrometry because pre-treatment of samples is not required. It can be used for direct analysis of solid and liquid samples with a simple mass spectrometer. We developed the novel Electrode-Assisted Desorption Electrospray Ionization (EADESI) method for quick biomedical sample analysis. EADESI is a novel ambient ionization method which can be used as detection of samples on nearly any type of substrate. The instrument consists of three major parts: a three-dimensional mo

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41

Chen, Wen-Tsen, and 陳玟岑. "Bioanalysis of Surface-Assisted Laser Desorption/Ionization Mass Spectrometry Using Nanomaterials." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/07053806639731332071.

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博士<br>國立臺灣大學<br>化學研究所<br>101<br>This thesis focuses on synthesizing nanomaterials through surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) for the analysis of captopril and catechins in urines and tea samples, respectively, and for the investigation of protein-protein interaction in addition to the detection of single- and double-stranded oligodeoxynucleotides (ss- and ds-ODNs, respectively). The thesis is divided into five parts. Chapter one introduces the framework and background of basic principles of mass spectrometry, the soft ionization method, MALDI, SALDI, gene

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42

Lee, Chuping, and 李竹平. "Mechanism of Matrix-Assisted Laser Desorption/Ionization (MALDI) and Its Applications." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/3gjhd5.

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博士<br>國立臺灣大學<br>化學研究所<br>106<br>Matrix-assisted laser desorption/ionization (MALDI) is one of the important methods in mass analysis for biomolecules. To improve the application of MALDI in bioanalysis, it is vital to understand the ionization mechanism. Here we study the three topics, which are the mechanism of protonated ion, the mechanism of metal-related ion separately, and then we compare some softer ionization methods to find out the method soft enough for some fragile molecules in MALDI. In the first topic of the protonated ion generation mechanism in MALDI, generating the first ions re

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Kuo, Shan-Jen, and 郭善仁. "Nanoparticle mass spectrometry with electrospray ionization and matrix-assisted laser desorption/ionization as the ion source." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/64788027228962549777.

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碩士<br>國立臺灣大學<br>化學研究所<br>90<br>The quadrupole ion trap (QIT) was first invented by Paul and Steinwedel in 1960. It has been used in various fields, for instance, mass analysis of atomic, molecular, and cluster ions. In commercially available ion trap mass spectrometer (ITMS), a radio-frequency (RF) ac field (typically 1MHz)is applied between the ring and the end-cap electrodes of the trap. This frequency limits the mass analysis range of ITMS. Traditionally, most of the ITMS are combined with microchannel plates (MCP) or channeltron electron multiplier (CEM) detectors. These kinds of mass spec

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44

Luehr, Teesha Crystal. "Multiplexed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) biomarker discovery." Thesis, 2017. https://dspace.library.uvic.ca//handle/1828/8913.

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The work presented herein is a method optimization for biomolecule detection and identification using Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI). MALDI-MSI is a unique form of mass spectrometry that is highly multiplexed; it can simultaneously retain location information of the mass of multiple ions, allowing for correlation of morphology or pathology to reconstructed ion heat maps. There were three main objectives for the research - 1) A method optimization of sample preparation techniques for bottom-up proteomic MALDI-MSI was performed. This included th

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Huang, Yu-Hui, and 黃郁惠. "Nanomaterials Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Oligodeoxynucleotide-Drug Complexes." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/43683345685577175283.

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碩士<br>國立臺灣大學<br>化學研究所<br>103<br>A surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using HgTe nanostructures as the matrix has been employed for the detection of four 15-base oligodeoxynucleotides (ODNs) and their complexes with mitoxantrone (MTX) that is a common drug for treatment of acute myeloid leukemia (AML) patients. The major peaks for the four tested ODNs are genes found in AML patients at m/z values of 4571, 4586, 4610, and 4635, while they are at m/z values of 5017, 5031, 5055, and 5079 for their corresponding complexes with MTX. The ODN with m/z value of 46

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Griffin, Timothy J. "Genetic analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry." 1999. http://catalog.hathitrust.org/api/volumes/oclc/43651475.html.

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47

Hsieh, Yi-Ting, and 謝依婷. "Detection of Monophosphate Nucleotides ThroughSurface-Assisted Laser Desorption/Ionization MassSpectrometry Using Gold Nanoparticles." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/73621759268878091646.

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碩士<br>臺灣大學<br>化學研究所<br>98<br>This thesis is that we demonstrated the determination of monophosphate nucleotides through the surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using hexadecyltrimethylammonium bromide (CTAB) adsorbed gold nanoparticles (CTAB-Au NPs). The binding of four monophosphate nucleotides — adenosine 5’-monophosphate (5’-AMP), guanosine 5’-monophosphate (5’-GMP),uridine 5’-monophosphate (5’-UMP) and cytidine 5’-monophosphate (5’-CMP) — to the surfaces of these CTAB-Au NPs induced their aggregation, which allowed selective concentration of the four a

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48

Wu, Chen-I., and 吳偵憶. "Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry for the Rapid Diagnosis of Occult Blood in Feces by Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/21585363718662964254.

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碩士<br>國立中山大學<br>化學系研究所<br>94<br>Abstract In this paper we demonstrate that matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry can be used to rapidly detect occult blood in human feces. The water-soluble hemoglobins, which are the most predominant proteins found in blood, were used as protein biomarkers for the presence of occult blood. The proteins were best extracted from feces using a small amount of pure water and then separated from the feces through centrifugation. We found that saturated 4-hydroxy-α-cyanocinnamic acid (α-CHC) solution was the best MA

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Fitzgerald, Michael C. "The analysis of nucleic acids by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry." 1994. http://catalog.hathitrust.org/api/volumes/oclc/31943244.html.

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Wu, Hsin-pin, and 吳欣頻. "The applications of metal nanoparticles : Biosensor and surface assisted laser desorption/ionization mass spectrometry." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/5dm8kk.

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碩士<br>國立中山大學<br>化學系研究所<br>96<br>The thesis is divided into four sections. 1. Sample-first preparation: A method for surface-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cyclic oligosaccharides：In this work, we report the application of a sample first preparation method for the analysis of cyclic oligosaccharides when using bare AuNPs as the assisted matrix. In the sample first method, the analyte is deposited first and then followed by the bare AuNPs. The cyclic oligosaccharides, including α-, β-, andγ-cycodextrin (CD), are difficult to ionize by MALDI-MS,

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