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Journal articles on the topic 'Pollen activation'

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1

Saporta, M., S. Kamei, L. Persi, J. Bousquet, and B. Arnoux. "Basophil activation during pollen season in patients monosensitized to grass pollens." Allergy 56, no. 5 (2001): 442–45. http://dx.doi.org/10.1034/j.1398-9995.2001.056005442.x.

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2

Gaspar, Ricardo, Mafalda Ramos de Matos, Luísa Cortes, et al. "Pollen Proteases Play Multiple Roles in Allergic Disorders." International Journal of Molecular Sciences 21, no. 10 (2020): 3578. http://dx.doi.org/10.3390/ijms21103578.

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Allergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and inflammatory responses that occur in patients with allergic disorders. In this study, we aimed to identify specific proteolytic activity in a set of pollens with diverse allergenic potential. Diffusates from Chenopodium album, Plantago lanceolata and Eucalyptus globulus were added to a confluent monolayer of Calu-3 cells grown in an air-liquid interface
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3

Breygina, Maria, Ekaterina Klimenko, Alexandra Podolyan, and Alexander Voronkov. "Dynamics of Pollen Activation and the Role of H+-ATPase in Pollen Germination in Blue Spruce (Picea pungens)." Plants 9, no. 12 (2020): 1760. http://dx.doi.org/10.3390/plants9121760.

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Pollen is a highly specialized structure for sexual plant reproduction. Early stages of pollen germination require the transition from dormant state to active metabolism. In particular, an important role during this early phase of angiosperm pollen germination is played by H+-ATPase. Very little is known about pollen activation in gymnosperm species, and information on the involvement of H+-ATPase is lacking. We tracked four indicators characterizing the physiological state of pollen: membrane potential, intracellular pH, anion efflux and oxygen uptake, in order to monitor the dynamics of acti
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4

Özdemir, Seçil Kepil, Deniz Güloğlu, Betül A. Sin, Atilla H. Elhan, Aydan İkincioğulları, and Zeynep Mısırlıgil. "Reliability of Basophil Activation Test Using CD203c Expression in Diagnosis of Pollen Allergy." American Journal of Rhinology & Allergy 25, no. 6 (2011): e225-e231. http://dx.doi.org/10.2500/ajra.2011.25.3723.

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Background CD203c is a basophil surface marker and its expression is rapidly up-regulated after cross-linking of high-affinity immunoglobulin E (IgE) receptor (FcepsilonR1) by an allergen. CD203c basophil activation tests have been studied for the in vitro diagnosis of several allergic conditions. However, there is limited data about its diagnostic usefulness. The optimum allergen concentrations for stimulation and allergen specific cutoff values remain unknown for a number of allergens. This study was designed to investigate the efficacy of basophil activation test via CD203c in the diagnosis
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5

Simons, Meagan A., and Adam R. Smith. "Ovary activation does not correlate with pollen and nectar foraging specialization in the bumblebeeBombus impatiens." PeerJ 6 (February 20, 2018): e4415. http://dx.doi.org/10.7717/peerj.4415.

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Social insect foragers may specialize on certain resource types. Specialization on pollen or nectar among honeybee foragers is hypothesized to result from associations between reproductive physiology and sensory tuning that evolved in ancestral solitary bees (the Reproductive Ground-Plan Hypothesis; RGPH). However, the two non-honeybee species studied showed no association between specialization and ovary activation. Here we investigate the bumblebeeB. impatiensbecause it has the most extensively studied pollen/nectar specialization of any bumblebee. We show that ovary size does not differ bet
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6

HÅkansson, Heinrich, Rak, and Venge. "Activation of B‐lymphocytes during pollen season. Effect of immunotherapy." Clinical & Experimental Allergy 28, no. 7 (1998): 791–98. http://dx.doi.org/10.1046/j.1365-2222.1998.00295.x.

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7

Mahmood, Faiza, Geir Hetland, Ivo Nentwich, Mohammad Reza Mirlashari, Reza Ghiasvand, and Lise Sofie Haug Nissen-Meyer. "Agaricus blazei-Based Mushroom Extract Supplementation to Birch Allergic Blood Donors: A Randomized Clinical Trial." Nutrients 11, no. 10 (2019): 2339. http://dx.doi.org/10.3390/nu11102339.

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Since Agaricus blazei Murill (AbM) extract reduced specific IgE and ameliorated a skewed Th1/Th2 balance in a mouse allergy model, it was tested in blood donors with self-reported, IgE-positive, birch pollen allergy and/or asthma. Sixty recruited donors were randomized in a placebo-controlled, double-blinded study with pre-seasonal, 7-week, oral supplementation with the AbM-based extract AndosanTM. Before and after the pollen season, questionnaires were answered for allergic rhino-conjunctivitis, asthma, and medication; serum IgE was measured, and Bet v 1-induced basophil activation was determ
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8

Zheng, Yang-Yang, Xian-Ju Lin, Hui-Min Liang, Fang-Fei Wang, and Li-Yu Chen. "The Long Journey of Pollen Tube in the Pistil." International Journal of Molecular Sciences 19, no. 11 (2018): 3529. http://dx.doi.org/10.3390/ijms19113529.

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In non-cleistogamous plants, the male gametophyte, the pollen grain is immotile and exploits various agents, such as pollinators, wind, and even water, to arrive to a receptive stigma. The complex process of pollination involves a tubular structure, i.e., the pollen tube, which delivers the two sperm cells to the female gametophyte to enable double fertilization. The pollen tube has to penetrate the stigma, grow in the style tissues, pass through the septum, grow along the funiculus, and navigate to the micropyle of the ovule. It is a long journey for the pollen tube and its two sperm cells be
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9

HESLOP-HARRISON, J., Y. HESLOP-HARRISON, M. CRESTI, A. TIEZZI, and F. CIAMPOLINI. "Actin During Pollen Germination." Journal of Cell Science 86, no. 1 (1986): 1–8. http://dx.doi.org/10.1242/jcs.86.1.1.

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The cytoplasm of the vegetative cell of the ungerminated pollen grain of Endymton non-scriplus and other angiosperm species contains numerous fusiform bodies sometimes exceeding 15μm in length and 2.5 μm in width, which bind fluorescent-labelled phalloidin and are likely therefore to constitute a storage form of actin. The bodies are dispersed during the activation of the pollen, being replaced by aggregates of slender phalloidin-binding fibrils, which converge towards the germination apertures and are present in the emerging pollen tube. The storage bodies appear to be homologous with crystal
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10

Gao, Qi-Fei, Li-Li Gu, Hui-Qin Wang, et al. "Cyclic nucleotide-gated channel 18 is an essential Ca2+ channel in pollen tube tips for pollen tube guidance to ovules in Arabidopsis." Proceedings of the National Academy of Sciences 113, no. 11 (2016): 3096–101. http://dx.doi.org/10.1073/pnas.1524629113.

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In flowering plants, pollen tubes are guided into ovules by multiple attractants from female gametophytes to release paired sperm cells for double fertilization. It has been well-established that Ca2+ gradients in the pollen tube tips are essential for pollen tube guidance and that plasma membrane Ca2+ channels in pollen tube tips are core components that regulate Ca2+ gradients by mediating and regulating external Ca2+ influx. Therefore, Ca2+ channels are the core components for pollen tube guidance. However, there is still no genetic evidence for the identification of the putative Ca2+ chann
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11

Ramanava, I. U., A. Y. Hancharou, and N. I. Dudarava. "Application of basophil activation and degranulation markers for diagnosis of pollen allergy." Proceedings of the National Academy of Sciences of Belarus, Medical series 15, no. 4 (2019): 405–13. http://dx.doi.org/10.29235/1814-6023-2018-15-4-405-413.

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There are several basophil activation and degranulation markers for basophil activation test discovered in recent years. However, only CD63 and CD203c are actively used. The activation and degranulation markers (CD11b, CD13, CD63, CD69, CD107a, CD164, CD203c and CD300a) were characterized from the point of view of the possibility to use for BAT and their clinical efficiency. It has been shown that the degranulation marker CD107a and the activation marker CD11b have high sensitivity and specificity and can be used to diagnose hypersensitivity to pollen allergens.
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12

Fujieda, Shigeharu, Ichiro Noda, Chizuru Sugimoto, Shigehito Mori, and Hitoshi Saito. "Effect of Pretreatment with Ketotifen on Pollinosis: Correlation with Eosinophil Cationic Protein." American Journal of Rhinology 8, no. 1 (1994): 49–54. http://dx.doi.org/10.2500/105065894781882675.

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In this study, we examined the effect of pretreatment with ketotifen on pollinosis, and correlations with the concentration of ECP in nasal discharge and the existence of eosinophils in nasal smear. Ketotifen was prophylactically administered to 74 patients with pollen allergy, and the treatment continued to the end of pollen season. An additional 25 patients with the same allergy commenced ketotifen treatment during the pollen season. The results showed that pretreatment with ketotifen reduced symptom scores during the pollen season, but did not suppress the increase in ECP concentration and
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13

Hwang, Jae-Ung, Ying Gu, Yong-Jik Lee, and Zhenbiao Yang. "Oscillatory ROP GTPase Activation Leads the Oscillatory Polarized Growth of Pollen Tubes." Molecular Biology of the Cell 16, no. 11 (2005): 5385–99. http://dx.doi.org/10.1091/mbc.e05-05-0409.

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Oscillation regulates a wide variety of processes ranging from chemotaxis in Dictyostelium through segmentation in vertebrate development to circadian rhythms. Most studies on the molecular mechanisms underlying oscillation have focused on processes requiring a rhythmic change in gene expression, which usually exhibit a periodicity of >10 min. Mechanisms that control oscillation with shorter periods (<10 min), presumably independent of gene expression changes, are poorly understood. Oscillatory pollen tube tip growth provides an excellent model to investigate such mechanisms. It is well
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14

Nakamura, Yuki, Koichi Kobayashi, and Hiroyuki Ohta. "Activation of galactolipid biosynthesis in development of pistils and pollen tubes." Plant Physiology and Biochemistry 47, no. 6 (2009): 535–39. http://dx.doi.org/10.1016/j.plaphy.2008.12.018.

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15

Metz, Martin, Stefanie Gilles, Astrid Geldmacher, Heidrun Behrendt, Claudia Traidl-Hoffmann, and Marcus Maurer. "Evidence for Non-Allergic Mast Cell Activation in Pollen-Associated Inflammation." Journal of Investigative Dermatology 131, no. 4 (2011): 987–90. http://dx.doi.org/10.1038/jid.2010.419.

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16

Eaves, Deborah J., Carlos Flores-Ortiz, Tamanna Haque, Zongcheng Lin, Nianjun Teng, and Vernonica E. Franklin-Tong. "Self-incompatibility in Papaver: advances in integrating the signalling network." Biochemical Society Transactions 42, no. 2 (2014): 370–76. http://dx.doi.org/10.1042/bst20130248.

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Self-fertilization, which results in reduced fitness of offspring, is a common problem in hermaphrodite angiosperms. To prevent this, many plants utilize SI (self-incompatibility), which is determined by the multi-allelic S-locus, that allows discrimination between self (incompatible) and non-self (compatible) pollen by the pistil. In poppy (Papaver rhoeas), the pistil S-determinant (PrsS) is a small secreted protein which interacts with the pollen S-determinant PrpS, a ~20 kDa novel transmembrane protein. Interaction of matching pollen and pistil S-determinants results in self-recognition, in
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17

Pablos, Isabel, Matthias Egger, Eva Vejvar, et al. "Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules." Medicina 55, no. 8 (2019): 504. http://dx.doi.org/10.3390/medicina55080504.

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Background and objectives: Pollens of weeds are relevant elicitors of type I allergies. While many Artemisia species occur worldwide, allergy research so far has only focused on Artemisia vulgaris. We aimed to characterize other prevalent Artemisia species regarding their allergen profiles. Materials and Methods: Aqueous extracts of pollen from seven Artemisia species were characterized by gel electrophoresis and ELISA using sera from mugwort pollen-allergic patients (n = 11). The cDNA sequences of defensin–proline-linked proteins (DPLPs) were obtained, and purified proteins were tested in a c
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18

Skilnyk, Hilary R., and John N. A. Lott. "Mineral analyses of storage reserves of Cucurbita maxima and Cucurbita andreana pollen." Canadian Journal of Botany 70, no. 3 (1992): 491–95. http://dx.doi.org/10.1139/b92-063.

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Cucurbita maxima and Cucurbita andreana are so closely related that hybridization is possible. These two species also have been shown to have very different levels of calcium storage in their seeds. Our neutron activation analysis studies have shown that the total amount of P, Ca, K, and Mg in pollen of the two species was similar. Energy dispersive X-ray analysis studies also showed that the composition of electron-dense particles in the tube cells of the two species was similar. Thus the differences in Ca levels in phytate reserves in the seeds of these two Cucurbita species do not appear to
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19

Heslop-Harrison, Y., and J. S. Heslop-Harrison. "The pollen tube activated abscission zone in the stigma of pearl millet: structural and physiological aspects." Canadian Journal of Botany 75, no. 7 (1997): 1200–1207. http://dx.doi.org/10.1139/b97-833.

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Abscission of the stigmas of pearl millet (Pennisetum typhoides (Burman) Stapf et Hubbard) after pollination results from the activation of a specialized zone of tissue near the base. This abscission zone comprises a well-defined stretch of the basal column made up of thin-walled cells lacking a cuticularized epidermis. The penetration of the first few pollen tubes following a compatible pollination results in loss of turgor throughout the tissue, followed by loss of adhesion between the cells and ultimately disruption of the whole zone. Pectolytic enzymes are released concomitantly, but the c
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20

Barycheva, Liudmila Yu, Liudmila V. Dushina, Marina V. Golubeva, Milana M. Minasyan, and Yulia N. Medvedenko. "Dynamics of basophil reactivity in the patients with allergic rhinitis on the background of allergen-specific immunotherapy." Russian Journal of Allergy 17, no. 4 (2021): 75–84. http://dx.doi.org/10.36691/rja1410.

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BACKGROUND: Allergen-specific immunotherapy (ASIT) helps to reduce spontaneous and to induce basophil reactivity, which is one of the major mechanisms of its effectiveness. A number of studies, though, have not confirmed the informative value of the basophil activation test (BAT), in case of ASIT, with house dust mites, meadow grass pollen, and wasp venom. BAT outcomes are likely to be influenced by the properties of the sensitizing allergen, the duration of ASIT, and the BAT markers that are used.
 AIMS: To evaluate the basophils reactivity in the patients sensitized to weed pollen and t
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21

Thomas, Steve, Kim Osman, Barend H. J. de Graaf, et al. "Investigating mechanisms involved in the self–incompatibility response in Papaver rhoeas." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 358, no. 1434 (2003): 1033–36. http://dx.doi.org/10.1098/rstb.2003.1288.

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Sexual reproduction in flowering plants is controlled by recognition mechanisms involving the male gametophyte (the pollen) and the female sporophyte (the pistil). Self–incompatibility (SI) involves the recognition and rejection of self– or incompatible pollen by the pistil. In Papaver rhoeas , SI uses a Ca 2+ –based signalling cascade triggered by the S –protein, which is encoded by the stigmatic component of the S –locus. This results in the rapid inhibition of incompatible pollen tube growth. We have identified several targets of the SI signalling cascade, including protein kinases, the act
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22

Brownfield, Lynette, Sarah Wilson, Ed Newbigin, Antony Bacic, and Steve Read. "Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes." Biochemical Journal 414, no. 1 (2008): 43–52. http://dx.doi.org/10.1042/bj20080693.

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The protein NaGSL1 (Nicotiana alata glucan synthase-like 1) is implicated in the synthesis of callose, the 1,3-β-glucan that is the major polysaccharide in the walls of N. alata (flowering tobacco) pollen tubes. Here we examine the production, intracellular location and post-translational processing of NaGSL1, and relate each of these to the control of pollen-tube callose synthase (CalS). The 220 kDa NaGSL1 polypeptide is produced after pollen-tube germination and accumulates during pollen-tube growth, as does CalS. A combination of membrane fractionation and immunoelectron microscopy revealed
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23

Eady, Colin, Keith Lindsey, and David Twell. "Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen." Plant Journal 5, no. 4 (1994): 543–50. http://dx.doi.org/10.1046/j.1365-313x.1994.5040543.x.

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24

Griffiths, P. T., J. S. Borlace, P. J. Gallimore, M. Kalberer, M. Herzog, and F. D. Pope. "Hygroscopic growth and cloud activation of pollen: a laboratory and modelling study." Atmospheric Science Letters 13, no. 4 (2012): 289–95. http://dx.doi.org/10.1002/asl.397.

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25

Abdelsamad, Ahmed, and Ales Pecinka. "Pollen-Specific Activation of Arabidopsis Retrogenes Is Associated with Global Transcriptional Reprogramming." Plant Cell 26, no. 8 (2014): 3299–313. http://dx.doi.org/10.1105/tpc.114.126011.

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26

Inuo, Chisato, Yasuto Kondo, Kenichi Tanaka, et al. "Japanese Cedar Pollen-Based Subcutaneous Immunotherapy Decreases Tomato Fruit-Specific Basophil Activation." International Archives of Allergy and Immunology 167, no. 2 (2015): 137–45. http://dx.doi.org/10.1159/000437325.

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27

Czarnobilska, Ewa, Małgorzata Bulanda, Daniel Bulanda, and Marcel Mazur. "The Influence of Air Pollution on the Development of Allergic Inflammation in the Airways in Krakow’s Atopic and Non-Atopic Residents." Journal of Clinical Medicine 10, no. 11 (2021): 2383. http://dx.doi.org/10.3390/jcm10112383.

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Until now, the simultaneous influence of air pollution assessed by measuring the objective marker of exposition (1-hydroxypirene, 1-OHP) and atopy on the development of allergic airway diseases has not been studied. The aim of this study was to determine the pathomechanism of the allergic response to PM2.5 in atopic and non-atopic patients. We investigated the changes in peripheral blood basophil activity of patients after stimulation with the birch pollen allergen alone, the allergen combined with PM2.5 (BP), PM2.5 alone, a concentration of 1-OHP in urine, and a distance of residence from the
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28

Smith, Adam R., Peter Graystock, and William O. H. Hughes. "Specialization on pollen or nectar in bumblebee foragers is not associated with ovary size, lipid reserves or sensory tuning." PeerJ 4 (October 27, 2016): e2599. http://dx.doi.org/10.7717/peerj.2599.

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Foraging specialization allows social insects to more efficiently exploit resources in their environment. Recent research on honeybees suggests that specialization on pollen or nectar among foragers is linked to reproductive physiology and sensory tuning (the Reproductive Ground-Plan Hypothesis; RGPH). However, our understanding of the underlying physiological relationships in non-Apisbees is still limited. Here we show that the bumblebeeBombus terrestrishas specialist pollen and nectar foragers, and test whether foraging specialization inB. terrestrisis linked to reproductive physiology, meas
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29

Heslop-Harrison, J., and Yolande Heslop-Harrison. "Vapour Phase Activation, Intracellular Motility and Germination in the Tripororate Pollen ofEpilobium angustifolium." Botanica Acta 106, no. 4 (1993): 331–37. http://dx.doi.org/10.1111/j.1438-8677.1993.tb00757.x.

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30

Xu, Xiaodong, Ligeng Ma, Ying Sun, and Daye Sun. "Activation effect of extracellular calmodulin on heterotrimeric G protein in pollen plasma membrane." Chinese Science Bulletin 44, no. 2 (1999): 190–91. http://dx.doi.org/10.1007/bf02884751.

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31

Dityatkovska, E. М., and S. V. Biletska. "Dynamics of class E and G immunoglobulins under the influence of combined allergen-specific immunotherapy in patients with allergic rhinitis with combined sensitization to pollen and household allergens." OTORHINOLARYNGOLOGY №5-6(3) 2020, no. 5-6(3) 2020 (November 4, 2020): 42–48. http://dx.doi.org/10.37219/2528-8253-2020-6-42.

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The aim of this study is to assess the dynamics of immunoglobulin E and G under the influence of combined allergen-specific immunotherapy (ASIT) in patients with allergic rhinitis with combined sensitization to pollen and household allergens. Materials and methods: The study involved 49 patients, residents of Dnipro city, of working age (35,5±1,5 years), of whom 24 (49,0%) - women and 25 (51,0%) - men. All patients were diagnosed with allergic rhinitis with average disease duration of 9,2 ±1,1 year, who underwent allergy examination - skin tests - prick test with pollen and household allergens
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32

Breygina, Maria, and Ekaterina Klimenko. "ROS and Ions in Cell Signaling during Sexual Plant Reproduction." International Journal of Molecular Sciences 21, no. 24 (2020): 9476. http://dx.doi.org/10.3390/ijms21249476.

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Pollen grain is a unique haploid organism characterized by two key physiological processes: activation of metabolism upon exiting dormancy and polar tube growth. In gymnosperms and flowering plants, these processes occur in different time frames and exhibit important features; identification of similarities and differences is still in the active phase. In angiosperms, the growth of male gametophyte is directed and controlled by its microenvironment, while in gymnosperms it is relatively autonomous. Recent reviews have detailed aspects of interaction between angiosperm female tissues and pollen
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33

Prisle, Nønne L., Jack J. Lin, Sara Purdue, Haisheng Lin, J. Carson Meredith, and Athanasios Nenes. "Cloud condensation nuclei activity of six pollenkitts and the influence of their surface activity." Atmospheric Chemistry and Physics 19, no. 7 (2019): 4741–61. http://dx.doi.org/10.5194/acp-19-4741-2019.

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Abstract. The role of surfactants in governing water interactions of atmospheric aerosols has been a recurring topic in cloud microphysics for more than two decades. Studies of detailed surface thermodynamics are limited by the availability of aerosol samples for experimental analysis and incomplete validation of various proposed Köhler model frameworks for complex mixtures representative of atmospheric aerosol. Pollenkitt is a viscous material that coats grains of pollen and plays important roles in pollen dispersion and plant reproduction. Previous work suggests that it may also be an import
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Pan, Changtian, Dandan Yang, Xiaolin Zhao, et al. "PIF4 negatively modulates cold tolerance in tomato anthers via temperature-dependent regulation of tapetal cell death." Plant Cell 33, no. 7 (2021): 2320–39. http://dx.doi.org/10.1093/plcell/koab120.

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Abstract Extreme temperature conditions seriously impair male reproductive development in plants; however, the molecular mechanisms underlying the response of anthers to extreme temperatures remain poorly described. The transcription factor phytochrome-interacting factor4 (PIF4) acts as a hub that integrates multiple signaling pathways to regulate thermosensory growth and architectural adaptation in plants. Here, we report that SlPIF4 in tomato (Solanum lycopersicum) plays a pivotal role in regulating cold tolerance in anthers. CRISPR (clustered regularly interspaced short palindromic repeats)
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35

Waldron, Gareth J., Stefan B. Sigurdsson, Ernesto A. Aiello, Andrew J. Halayko, Newman L. Stephens, and William C. Cole. "Delayed rectifier K+ current of dog bronchial myocytes: effect of pollen sensitization and PKC activation." American Journal of Physiology-Lung Cellular and Molecular Physiology 275, no. 2 (1998): L336—L347. http://dx.doi.org/10.1152/ajplung.1998.275.2.l336.

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The properties of delayed rectifier K+ current [ I K(dr)] of canine airway smooth muscle cells isolated from small bronchi and its modulation by protein kinase C (PKC) were studied by whole cell patch clamp. I K(dr) activated positive to −40 mV, with half-maximal activation at −16 ± 1.2 mV ( n = 15) and average current density of 31 ± 2.6 pA/pF ( n = 15) at +30 mV. The capacitive surface area, current density, and voltage dependence of activation of I K(dr) of myocytes of ragweed pollen-sensitized dogs were not different from age-matched control dogs. However, the sensitization reduced the ava
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36

Cheng, Ziyi, Xiaorui Guo, Jiaxue Zhang та ін. "βVPE is involved in tapetal degradation and pollen development by activating proprotease maturation in Arabidopsis thaliana". Journal of Experimental Botany 71, № 6 (2019): 1943–55. http://dx.doi.org/10.1093/jxb/erz560.

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Abstract Vacuolar processing enzyme (VPE) is responsible for the maturation and activation of vacuolar proteins in plants. We found that βVPE was involved in tapetal degradation and pollen development by transforming proproteases into mature protease in Arabidopsis thaliana. βVPE was expressed specifically in the tapetum from stages 5 to 8 of anther development. The βVPE protein first appeared as a proenzyme and was transformed into the mature enzyme before stages 7–8. The recombinant βVPE protein self-cleaved and transformed into a 27 kDa mature protein at pH 5.2. The mature βVPE protein coul
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37

Kodad, Ossama, Rafel Socias i Company, Ana Sánchez, and M. Margarida Oliveira. "The Expression of Self-compatibility in Almond May Not Only Be Due to the Presence of the Sf Allele." Journal of the American Society for Horticultural Science 134, no. 2 (2009): 221–27. http://dx.doi.org/10.21273/jashs.134.2.221.

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The pistil (S-RNase) and the pollen [S-haplotype-specific F-box protein (SFB)] components of the Sf allele, presumably conferring self-compatibility in almond {Prunus amygdalus Batsch [syn. P. dulcis (Mill.) D.A. Webb]}, were identified and sequenced in ‘Ponç’, a local Spanish almond cultivar, confirming their identity with the published sequences of these components. Despite the presence of the Sf allele, the ‘Ponç’ phenotype was self-incompatible as confirmed by different pollination tests, including self pollen tube growth, fruit set after self-pollination, and fruit set in bagged branches.
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38

Shrestha, Ankita, Ajay Kumar Mishra, Jaroslav Matoušek, et al. "Integrated Proteo-Transcriptomic Analyses Reveal Insights into Regulation of Pollen Development Stages and Dynamics of Cellular Response to Apple Fruit Crinkle Viroid (AFCVd)-Infection in Nicotiana tabacum." International Journal of Molecular Sciences 21, no. 22 (2020): 8700. http://dx.doi.org/10.3390/ijms21228700.

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Tobacco (Nicotiana tabacum) pollen is a well-suited model for studying many fundamental biological processes owing to its well-defined and distinct development stages. It is also one of the major agents involved in the transmission of infectious viroids, which is the primary mechanism of viroid pathogenicity in plants. However, some viroids are non-transmissible and may be possibly degraded or eliminated during the gradual process of pollen development maturation. The molecular details behind the response of developing pollen against the apple fruit crinkle viroid (AFCVd) infection and viroid
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Traidl-Hoffmann, Claudia, Valentina Mariani, Hubertus Hochrein, et al. "Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization." Journal of Experimental Medicine 201, no. 4 (2005): 627–36. http://dx.doi.org/10.1084/jem.20041065.

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Pollen grains induce allergies in susceptible individuals by release of allergens upon contact with mucosal membranes of the upper respiratory tract. We recently demonstrated that pollen not only function as allergen carriers but also as rich sources of bioactive lipids that attract cells involved in allergic inflammation such as neutrophils and eosinophils. Here we demonstrate that soluble factors from birch (Betula alba L.) pollen activate human dendritic cells (DCs) as documented by phenotypical and functional maturation and altered cytokine production. Betula alba L. aqueous pollen extract
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Xu, Yuanyuan, Xirong Cao, Haoan Zhao, et al. "Impact of Camellia japonica Bee Pollen Polyphenols on Hyperuricemia and Gut Microbiota in Potassium Oxonate-Induced Mice." Nutrients 13, no. 8 (2021): 2665. http://dx.doi.org/10.3390/nu13082665.

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Camellia japonica bee pollen is one of the major types of bee pollen in China and exhibits antioxidant and anti-inflammatory activities. The aims of our study were to evaluate the effects and the possible mechanism of Camellia japonica bee pollen polyphenols on the treatment of hyperuricemia induced by potassium oxonate (PO). The results showed that Camellia japonica bee pollen ethyl acetate extract (CPE-E) owned abundant phenolic compounds and strong antioxidant capabilities. Administration with CPE-E for two weeks greatly reduced serum uric acid and improved renal function. It inhibited live
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Kasai, Y., C. Leipe, M. Saito, et al. "Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter." Science Advances 7, no. 16 (2021): eabe7327. http://dx.doi.org/10.1126/sciadv.abe7327.

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Particle sorting is a fundamental method in various fields of medical and biological research. However, existing sorting applications are not capable for high-throughput sorting of large-size (>100 micrometers) particles. Here, we present a novel on-chip sorting method using traveling vortices generated by on-demand microjet flows, which locally exceed laminar flow condition, allowing for high-throughput sorting (5 kilohertz) with a record-wide sorting area of 520 micrometers. Using an activation system based on fluorescence detection, the method successfully sorted 160-micrometer microbead
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Chang, Fang, Ying Gu, Hong Ma, and Zhenbiao Yang. "AtPRK2 Promotes ROP1 Activation via RopGEFs in the Control of Polarized Pollen Tube Growth." Molecular Plant 6, no. 4 (2013): 1187–201. http://dx.doi.org/10.1093/mp/sss103.

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Li, H., A. Bacic, and S. M. Read. "Activation of Pollen Tube Callose Synthase by Detergents (Evidence for Different Mechanisms of Action)." Plant Physiology 114, no. 4 (1997): 1255–65. http://dx.doi.org/10.1104/pp.114.4.1255.

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Plötz, Sabine G., Claudia Traidl-Hoffmann, Ivo Feussner, et al. "Chemotaxis and activation of human peripheral blood eosinophils induced by pollen-associated lipid mediators." Journal of Allergy and Clinical Immunology 113, no. 6 (2004): 1152–60. http://dx.doi.org/10.1016/j.jaci.2004.03.011.

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Choi, Seung Wan, Jialiang Tang, Vilas G. Pol, and Ki Bong Lee. "Pollen-derived porous carbon by KOH activation: Effect of physicochemical structure on CO2 adsorption." Journal of CO2 Utilization 29 (January 2019): 146–55. http://dx.doi.org/10.1016/j.jcou.2018.12.005.

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Mikhailov, E. F., O. A. Ivanova, E. Yu Nebosko, S. S. Vlasenko, and T. I. Ryshkevich. "Subpollen particles as atmospheric cloud condensation nuclei." Известия Российской академии наук. Физика атмосферы и океана 55, no. 4 (2019): 64–72. http://dx.doi.org/10.31857/s0002-351555464-72.

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Bioparticles represent a significant fraction of the total atmospheric aerosol. Their size range varies from nanometers (macromolecules) to hundreds of micrometers (plant pollen, vegetation residues) and like other atmospheric aerosol particles, the degree of involvement of bioaerosols in atmospheric processes largely de- pends on their hygroscopic and cloud condensation nuclei properties. In this paper the ability of the pine, birch and rape subpollen particles to act as cloud condensation nuclei are considered. Submicron particles were obtained by aqueous extraction of biological material fr
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Jedrzejczak-Czechowicz, Monika, Anna Lewandowska-Polak, Marzanna Jarzebska, and Marek L. Kowalski. "Mast cell and eosinophil activation during early phase of grass pollen‐induced ocular allergic reaction." Allergy and Asthma Proceedings 32, no. 1 (2011): 43–48. http://dx.doi.org/10.2500/aap.2011.32.3402.

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Breygina, M. A., N. P. Matveyeva, D. S. Andreyuk, and I. P. Yermakov. "Transmembrane transport of K+ and Cl− during pollen grain activation in vivo and in vitro." Russian Journal of Developmental Biology 43, no. 2 (2012): 85–93. http://dx.doi.org/10.1134/s1062360412020038.

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Takaishi, Shinya, Saburo Saito, Tomonori Endo, et al. "T‐cell activation by transgenic rice seeds expressing the genetically modified Japanese cedar pollen allergens." Immunology 158, no. 2 (2019): 94–103. http://dx.doi.org/10.1111/imm.13097.

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Bosch, M., and V. E. Franklin-Tong. "Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen." Proceedings of the National Academy of Sciences 104, no. 46 (2007): 18327–32. http://dx.doi.org/10.1073/pnas.0705826104.

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