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Journal articles on the topic 'Pollen viability'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Costa, Paulo Marks de Araújo, Vênia Camelo de Souza, Ivan Sérgio da Silva Oliveira, Vagner Sousa da Costa, and Emerson Serafim Barros. "Pollen viability and floral biology of Mandacaru (Cereus jamacaru (DC) (Cactaceae))." Research, Society and Development 9, no. 8 (2020): e997986671. http://dx.doi.org/10.33448/rsd-v9i8.6671.

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Pollen fertility is indicated by viability, which influences the reproductive success of plants. Mandacaru is a native tree species with natural distribution in the Caatinga. The objective was to verify the pollen viability, pollen per ovule ratio and the morphological identification of the mandacaru flower (Cereus jamacaru). Ten flower buds were used and one hundred anthers of these buds, which and stained with Alexander dye on checkered glass slide and viable and non-viable pollens were accounted amount of ovule per flower bud was counted the pollen/ovule ratio. Mandacaru presented viability

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2

Aswath, VivekC R., N. S. Bhandari, and S. Gangola. "SEM-based study for palynological and pollen germination of Lilium longiflorum cv. Pavia." Journal of Applied Horticulture 24, no. 03 (2023): 302–5. http://dx.doi.org/10.37855/jah.2022.v24i03.53.

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Pollen is the first choice of germplasm curator, geneticist, breeder and physiologist for conservation and crop improvement programs. A pollen palynological attributes and pollen germination study of Lilium longiflorum cv., 'Pavia' was conducted using scanning electron microscope (SEM). Various morphological characteristics were studied. The results showed that pollens were much alike in ultra-morphology with elliptical to ellipsoidal shape, reticulate exine and single germination ditch. The pollen viability was tested by the wet room method with 3 dyes; Acetocarmine, TTC, Lugol's iodine and 4

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3

Öztürk Çalı, İ. "The effects of fosetyl-Al application on morphology and viability of Lycopersicon esculentum Mill. pollen." Plant, Soil and Environment 54, No. 8 (2008): 336–40. http://dx.doi.org/10.17221/414-pse.

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In the present study, the effects of fungicide Aliette WG 800 [80% fosetyl-Al (aluminium tris-o-ethyl phosphonate)], widely used against <I>Phytophtora infestans</I> on tomatoes grown in greenhouse in Turkey, were studied on the morphology and viability of tomato (<I>Lycopersicon esculentum</I> Mill.) pollens. The fungicide was applied to tomatoes grown in greenhouse at recommended dosage (200 g/100 l water) and at double the recommended dosage (400 g/100 l water). The fungicide caused changes in the morphological structures of tomato pollens. Some pollen morphological

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4

Du, Xiaohua. "Pollen Ultra-Morphology and Pollen Viability Test of Lilium Oriental Hybrids." International Journal of Agriculture and Biology 20, no. 08 (2020): 1903–7. http://dx.doi.org/10.17957/ijab/15.0753.

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Ultra-morphology and pollen viability assessment are essential to understand evolutionary ecology and sterility problem, and to design hybridization program in cross-breeding. The pollen ultra-morphology of two Lilium Oriental hybrid cultivars, „Sorbonne‟ and „Siberia‟ were observed using scanning electron microscope. And the pollen germination condition in vitro and four dye tests methods for pollen viability were determined. The results showed that pollens of two Lilium Oriental hybrid cultivars were much alike in ultra-morphology with ellipsoidal shape, reticulate surface decoration and sin

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5

Shivanna, K. R., H. F. Linskens, and M. Cresti. "Pollen viability and pollen vigor." Theoretical and Applied Genetics 81, no. 1 (1991): 38–42. http://dx.doi.org/10.1007/bf00226109.

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6

Audu, M. A. I., O. A. Falusi, M. L. Muhammad, O. A. Y. Daudu, and A. Abubakar. "Pollen Viability and Germinability of Gamma Irradiated M4 Lines of Sesame." BADEGGI JOURNAL OF AGRICULTURAL RESEARCH AND ENVIRONMENT 3, no. 2 (2021): 11–18. http://dx.doi.org/10.35849/bjare202102002.

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Pollen viability and germinability are indispensable criteria in plant breeding programmes. This research evaluated eleven M4 mutant lines of gamma irradiated sesame (Sesamum indicum) for some pollen parameters. Seeds were obtained from the Department of Plant Biology, Federal University of Technology, Minna and were raised to maturity alongside their respective checks in a randomized complete block design (RCBD). The field experiment was conducted at the experimental field of the Upper Niger River Basin Development Authority, Minna. Pollen viability and diameter were determined using standard

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7

Devrnja, Nina, Jelena Milojević, Ljiljana Tubić, Snežana Zdravković-Korać, Aleksandar Cingel, and Dušica Ćalić. "Pollen Morphology, Viability, and Germination of Tanacetum vulgare L." HortScience 47, no. 3 (2012): 440–42. http://dx.doi.org/10.21273/hortsci.47.3.440.

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The pollen morphology of aromatic Tanacetum vulgare L. (Asteraceae), which has wide horticultural and medicinal uses, was examined using light microscopy and scanning electron microscopy. The investigation revealed that pollen grains are radially symmetrical, isopolar, spheroidal, tricolporate, and echinate–perforate. Average pollen length was 21.32 ± 1.12 μm, whereas average pollen width was 20.04 ± 0.98 μm (length:width ratio 0.94). Spine length was 2.72 ± 0.29 μm. Average distance from the spines was 7.15 ± 0.31 μm. Pollen exine consisted of 1–3 pores. Pori are elongated and also with a dis

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8

Lamo, Kunzang, Anil Kumar Sangwan, Nav Prem Singh, and Manveen Kaur Batth. "Optimization of invitro Pollen Storage Conditions in Seeded and Low Seeded Citrus Genotypes." International Journal of Current Microbiology and Applied Sciences 12, no. 9 (2022): 141–58. http://dx.doi.org/10.20546/ijcmas.2023.1209.014.

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In vitro pollen storage study was conducted using pollens of three seeded citrus genotypes viz., ‘Mexican lime’,’ W.Murcott’ and ‘Mosambi’ and five seedless citrus genotypes viz., ‘Lisbon lemon’, ‘Jaffa’, ‘Clementine’, ‘Hamlin’ and ‘Mukaku Kishu’. Pollen viability and germination percentage were evaluated at different storage temperature treatments i.e., at room temperature (in anhydrous calcium chloride) (control), refrigerator 40C, freezer (-200C), freeze drier (-800C). In vitro pollen viability capacity was tested with acetocarmine stain (2%). Among all the tested sucrose concentration (0,

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9

Stern, Raphael A., and Shmuel Gazit. "Pollen Viability in Lychee." Journal of the American Society for Horticultural Science 123, no. 1 (1998): 41–46. http://dx.doi.org/10.21273/jashs.123.1.41.

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The lychee (Litchi chinensis Sonn.) has two types of pollen-releasing flowers—M1 and M2. We compared the morphology and viability of these two pollen types, mainly for the two commercial cultivars in Israel: `Mauritius' and `Floridian'. Observation by scanning electron microscope did not reveal any consistent morphological differences between the two pollen types. However, M2 pollen was found to have a consistent and significant advantage over M1 pollen in in vitro germination tests. M2 pollen from `Mauritius', `Floridian', `No Mai Chee', `Wai Chee', and `Early Large Red' had a much higher ger

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10

Demirel, Mehmet Akif, Kenan Yıldız, and Cevriye Mert. "Pollen Performance and Morphology of Black Mulberry (Morus nigra L.) Genotypes." Akademik Ziraat Dergisi 14, no. 1 (2025): 39–47. https://doi.org/10.29278/azd.1512106.

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Objective: It was aimed to determine the morphology, viability, germination rates, and production quantity of black mulberry (Morus nigra L.) pollens, which play an important role in pollination and fertilization. Materials and Methods: In this research, pollen viability of black mulberry pollens using the TTC method, pollen germination test using the “agar–plate” method, pollen quantity using the Hemocytometric method, pollen sizes and morphologies using light microscope and scanning electron microscope (SEM) were examined. Results: In this study, pollen performance and morphological structur

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11

Zhao, Rui, Linjie Xu, Xiangshuai Xu, Yanmin Li, Shixin Xiao, and Deyi Yuan. "Comparative Study on Pollen Viability of Camellia oleifera at Four Ploidy Levels." Agronomy 12, no. 11 (2022): 2592. http://dx.doi.org/10.3390/agronomy12112592.

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Oil tea (Camellia oleifera Abel.) is one of the most important woody edible oil tree species in China, with intraspecific polyploid. In order to study the variation in pollen size and vigor of C. oleifera at ploidy level, four ploidy covers a total of 32 types of Camellia pollens as the material for the experiment. The results showed that the pollen sizes of diploid, tetraploid, hexaploidy, and octaploid were positively correlated with the ploidy level. Pollen viability of C. oleifera was determined by fluorescein diacetate (FDA) dye solution staining and medium containing 10% sucrose, 0.01% b

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12

Munir, Muhammad. "An appraisal of pollen germination and viability of varied male pollen sources of date palm (Phoenix dactylifera L.)." Journal of Applied Horticulture 23, no. 03 (2021): 254–59. http://dx.doi.org/10.37855/jah.2021.v23i03.45.

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Date palm is a dioecious fruit tree that permits cross pollination for fruit setting. The germination of viable pollens to fertilize ovule is influenced by environmental cues such as temperature. The germination and viability of pollen grains collected for pollination purpose also varied with the male pollinizer source and the male spathe opening time. An in vitro study performed to determine the percentage of pollen germination at different temperatures (10, 15, 20, 25, and 30 °C) and germination times (4, 8, 12, and 24 h) taken from different male sources and toidentify any variations in the

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13

Lamo, Kunzang, Padma Gurmet, and Tsewang Rinchen. "Studies on pollen performance in some endangered medicinal and aromatic plants of cold desert Ladakh." Environment Conservation Journal 25, no. 3 (2024): 717–22. http://dx.doi.org/10.36953/ecj.27132801.

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The study was carried out at the Trans-Himalayan Herbal Garden, located at the National Institute of Sowa Rigpa-Leh, from 2021 to 2023. An assessment of pollen viability was conducted using seven significant medicinal and aromatic plants from the Ladakh region. Analysis of pollen vitality was conducted using the acetocarmine staining technique. The pollen viability of Inula racemosa Hook. f. ranged from 91.08 ± 1.39 % to 82.44 ± 1.48 % in Ephedra gerardiana Wall. Among the genus Inula racemosa, the highest percentage (91.08%) of viable pollens indicates a normal meiosis, whereas Ephedra gerard

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14

Ridhawati, A., W. M. Mahayu, G. Garusti, and N. Asbani. "Viability of sugarcane and Erianthus arundinaceus pollen under marcotting treatment." IOP Conference Series: Earth and Environmental Science 974, no. 1 (2022): 012053. http://dx.doi.org/10.1088/1755-1315/974/1/012053.

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Abstract Most of the sugarcane cultivated today is the hybrid between Saccharum offinarum and its relative, S. spontaneum. The breeding relies mainly on the crossing of male and female parents, which needs the excellent viability of the pollen from the male parent. Marcotting or air layer is a technique to facilitate parents to be crossed outside of the sugarcane fields. The technique allows sugarcane roots to grow from stalk internodes wrapped with soil to maintain the viability of the male and female gametes during the crossing. We investigated the effect of marcotted stalks on pollen viabil

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15

Abdul-Baki, Aref A. "Determination of Pollen Viability in Tomatoes." Journal of the American Society for Horticultural Science 117, no. 3 (1992): 473–76. http://dx.doi.org/10.21273/jashs.117.3.473.

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A procedure is described for determining pollen viability in tomatoes (Lycopersicon esculentum Mill.) by growing pollen in a growth medium containing 0.29 M sucrose, 1.27 mm Ca(NO3)2, 0.16 mm H3 BO3, and 1 mm KNO3 (pH 5.2) to which 0.001% fluorescein diacetate (FDA) was added. Pollen viability can be evaluated within 30 min by determining percent fluorescing pollen in a sample. The procedure further allows the determination of percent germination in vitro and pollen tube growth within 1.5 hours. Neither the germination medium nor FDA has any adverse effects on germination and pollen tube growt

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16

Hebert*, Cary, Jeff Kuehny, Charles Johnson, and Annina Delaune. "Pollen Viability of Clerodendrum." HortScience 39, no. 4 (2004): 808A—808. http://dx.doi.org/10.21273/hortsci.39.4.808a.

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The genus Clerodendrum belongs to the family Verbenaceae of which there are over 400 tree, shrub, and vine species. Species of Clerodendrum vary in leaf size, shape and texture; inflorescence shape; and flower shape, size and color. There is commercial interest in developing hybrids with desirable floricultural attributes. Interspecific hybridization could be used to increase variability in flower color, inflorescence shape, plant vigor, leaf color and shape for selection. Pollen viability among species is in question because of absence of seed set on many selected plants. The need for assessi

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17

Pravallika, Rai Narayana, and Shama Parveen. "Evaluation of Growth and Pollen Viability (Fertility) in Relation to Fruit Set among Five Varieties of Tomato." International Journal of Environment and Climate Change 13, no. 6 (2023): 88–94. http://dx.doi.org/10.9734/ijecc/2023/v13i61801.

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The evaluation of pollen viability and fruit setting capacity are two essential criteria for pollinator’s characterization. This study was carried out to evaluate pollen Fertility/ viability in five Varieties of Tomatoes. We analyzed the viability of pollen grains of five different varieties of tomatoes grown in ITM University, Gwalior, Madhya Pradesh, India. Aceto- carmine method is used to estimate pollen viability. The germination percentage, pollen viability and Fruit setting rate were recorded at different stages respectively. Pollen viability in relation to fruit set was analyzed while g

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18

Hecker, R. J., and M. McClintock. "Sugarbeet Pollen Viability Indicators." Journal of Sugarbeet Research 26, no. 3&4 (1989): 40–48. http://dx.doi.org/10.5274/jsbr.26.3.40.

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19

França, L. V., W. M. Nascimento, R. Carmona, and R. A. Freitas. "Viability of eggplant pollen." Cropp Breeding and Applied Biotechnology 9, no. 4 (2009): 320–27. http://dx.doi.org/10.12702/1984-7033.v09n04a06.

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Batos, Branislava, and Danijela Miljkovic. "Pollen viability in Quercus robur L." Archives of Biological Sciences 69, no. 1 (2017): 111–17. http://dx.doi.org/10.2298/abs160121084b.

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The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at

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21

Е.В., БАЖИНА, та СЕДАЕВА М.И. "ЖИЗНЕСПОСОБНОСТЬ ПЫЛЬЦЫ PICEA OBOVATA LEDEB. В УСЛОВИЯХ ТЕХНОГЕННОГО СТРЕССА". Сибирский экологический журнал 30, № 5 (2023): 705–14. http://dx.doi.org/10.15372/sej20230511.

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Результаты сравнительного исследования пыльцы ели сибирской в фоновых условиях и при повышенном уровне загрязнения (граница крупного промышленного центра - г. Красноярска) показали, что динамика развития и жизнеспособность пыльцы варьируют в зависимости от погодных условий года развития в периоды формирования пыльцевых зерен и пыления, техногенного загрязнения. В условиях повышения уровня техногенной нагрузки на границе с городом снижаются прорастание пыльцы, длина пыльцевых трубок, накопление основного питательного вещества - крахмала. The Siberian spruce (Picea obovata Ledeb.) pollen viabili

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Fortescue, J. A., and D. W. Turner. "Pollen fertility in Musa: Viability in cultivars grown in Southern Australia." Australian Journal of Agricultural Research 55, no. 10 (2004): 1085. http://dx.doi.org/10.1071/ar04078.

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Pollen viability was examined by recording the number of viable as well as non-viable microspores in anthers just before anthesis, as it could be an efficient and rapid indicator of female fertility. It was thought that competency in meiotic restitution in microspores could indicate similar competency in megaspores. Pollen fertility was compared among seeded diploids and edible triploids of Musa and correlated with ovule fertility. Viability was examined using the Alexander’s pollen stain procedure. The seeded diploid species M. acuminata, M. balbisiana, and M. ornata had 3 times more viable p

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Freeman, Josh H., Stephen M. Olson, and Eileen A. Kabelka. "Pollen Viability of Selected Diploid Watermelon Pollenizer Cultivars." HortScience 43, no. 1 (2008): 274–75. http://dx.doi.org/10.21273/hortsci.43.1.274.

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In the Spring and Fall 2006, the pollen viability of four diploid watermelon pollenizers was evaluated in Quincy, FL. Triploid watermelon plants [Citrullus lanatus (Thunb.) Matsum. & Nakai.] do not produce sufficient viable pollen to pollenize themselves and a diploid cultivar must be interplanted as a pollen source. Recent studies have illustrated differences in triploid watermelon yields as a result of the pollenizer cultivar used. The viability of the pollen produced by pollenizer cultivars may greatly influence the fruit set and fruit quality in the triploid watermelon crop. Pollen sam

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De Freitas, Jéssica Mena Barreto, Andrielle Wouters Kuhn, Viviane Dal-Souto Frescura, Liliana Essi, and Solange Bosio Tedesco. "Differences in stomatal and pollen grain dimensions and pollen viability between Paspalum rawitscheri populations." Ciência e Natura 42 (June 29, 2020): e46. http://dx.doi.org/10.5902/2179460x40749.

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The objective of this study was to evaluate stomatal and pollen grain size and to estimate pollen viability of individuals from different populations of Paspalum rawitscheri (Parodi) Chase ex G.H. Rua Valls. To analyze stomatal size, slides were made of the adaxial leaf epidermis using the epidermal impression method. The height and width of 100 stomata per population were analyzed. Pollen was obtained from inflorescences to evaluate pollen grain size and pollen viability. Pollen grains were stained with 2% acetic orcein, 2% acetic carmine, or Alexander’s reactive stain. Per population, 1600 g

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El Mardi, M. O., E. Consolacion, O. AI-Manthari, and H. AI-Nabhani. "Factors Influencing Date Pollen Viability." Journal of Agricultural and Marine Sciences [JAMS] 2 (January 1, 1997): 55. http://dx.doi.org/10.24200/jams.vol2iss0pp55-58.

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Pollen of seven date cultivars was stored at 4.5 -5.0'C using desiccator with or without silica gel and in vials outside the desicator as a control. Germination percentage was determined microscopically with a hemacytometer after harvest and at three and six months of Storage, respectively, using a boric acid-sucrosce medium. Cultivar genotype did out influence the viability of pollen. Pollen stored for six months gave a higher germination percentage (45.9%) than three months (40.9%) indicating that pollen requires more than three months at 4.5 - 5C for the reactivation or its growth factors.

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Buchner, Lisa, Anna-Katharina Eisen, Branko Šikoparija, and Susanne Jochner-Oette. "Pollen Viability of Fraxinus excelsior in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport." Forests 13, no. 4 (2022): 600. http://dx.doi.org/10.3390/f13040600.

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Fragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of pollen affects pollen viability was investigated with experiments in a climate chamber using ash pollen samples from a seed orchard in Emmendingen, Germany. Furthermore, experiments with a volumetric pollen trap were conducted. A suitable storage temperature for ash pollen was determined by using four viability tests; TTC test, pollen germination, Alexa

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Barabé, Denis, Karine Lavallée, and Marc Gibernau. "Pollen viability and germination in some neotropical aroids." Botany 86, no. 1 (2008): 98–102. http://dx.doi.org/10.1139/b07-110.

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Pollen viability and germination were observed in six species of neotropical Araceae. In Anaphyllopsis americana (Engl.) A. Hay, 50% of pollen grains remain viable after 70 h following dehiscence, and it takes over 210 h for total loss of viability to occur. In Montrichardia arborescens (L.) Schott, approximately 50% of pollen grains are not viable after 24 h, and no germination occurs after 36 h. Monstera adansonii Schott and Philodendron pedatum (Hook.) Kunth have the lowest initial pollen viability (40%–55%) and lose half of this viability after approximately 30 h. Pollen grains of Monstera

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Impe, Daniela, and Manuela Nagel. "Wheat pollen viability and feasability of pollen storage." Cryobiology 85 (December 2018): 151. http://dx.doi.org/10.1016/j.cryobiol.2018.10.125.

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Pan, Cheng Chen, Lin De Liu, Ha Lin Zhao, et al. "Pollen Biology of Robinia pseudoacacia on Zhen Mountain in Yantai." Advanced Materials Research 356-360 (October 2011): 2748–51. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.2748.

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The pollen–ovule ratios, pollen fertility rate, pollen viability and longevity, pollen histochemistry of Robinia pseudoacacia flowers were determined in the field on Zhen Mountain in Yantai, Shandong province in China. The main results were as follows: Robinia pseudoacacia is completely fertile and has starchy pollen. The pollen longevity was about 80h. The pollen viability was higher than 90% at the beginning of anthesis on sunny days. 80h later, most pollen grains lose their viability. Pollen–ovule ratio (P/O) is 192±19. Accoring to Cruden, R. pseudoacacia is facultative autogamous.

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D’Antonio, Vince, and Carlos F. Quiros. "Viability of Celery Pollen After Collection and Storage." HortScience 22, no. 3 (1987): 479–81. http://dx.doi.org/10.21273/hortsci.22.3.479.

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Abstract A technique for collection and storage of viable celery (Apium graveolens L.) pollen is reported for the cv. Tall Utah 52-70R and the annual strain A143. Umbels at anthesis were dried in an incubator for 14 hr at 31°C, crushed by hand, and passed through a sieve. The pollen released by this operation was collected on a sheet of paper and stored in gelatin capsules. High drying temperatures were detrimental to pollen viability. Pollen collection at different times of the day did not affect viability. After 6 days at 24°, a significant percentage of pollen grains was still viable. The v

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van der Walt, I. David, and Gail M. Littlejohn. "Storage and Viability Testing of Protea Pollen." Journal of the American Society for Horticultural Science 121, no. 5 (1996): 804–9. http://dx.doi.org/10.21273/jashs.121.5.804.

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The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that

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Tan, Zhihao, Jing Yang, Qingyuan Li, et al. "PollenDetect: An Open-Source Pollen Viability Status Recognition System Based on Deep Learning Neural Networks." International Journal of Molecular Sciences 23, no. 21 (2022): 13469. http://dx.doi.org/10.3390/ijms232113469.

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Pollen grains, the male gametophytes for reproduction in higher plants, are vulnerable to various stresses that lead to loss of viability and eventually crop yield. A conventional method for assessing pollen viability is manual counting after staining, which is laborious and hinders high-throughput screening. We developed an automatic detection tool (PollenDetect) to distinguish viable and nonviable pollen based on the YOLOv5 neural network, which is adjusted to adapt to the small target detection task. Compared with manual work, PollenDetect significantly reduced detection time (from approxim

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Mulugeta, Dawit, Bruce D. Maxwell, Peter K. Fay, and William E. Dyer. "Kochia (Kochia scoparia) Pollen Dispersion, Viability and Germination." Weed Science 42, no. 4 (1994): 548–52. http://dx.doi.org/10.1017/s004317450007692x.

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Kochia pollen dispersion was measured during 24 and 48 h periods from a kochia population in an 8- by 10-m area in the center of a 1.6 ha fallow field. Pollen counts from traps at 50- and 100-cm heights declined rapidly with increasing distance from the pollen source. Pollen deposition was highest along the prevailing wind direction: up to 23 pollen grains cm–2were recovered 50 m from the pollen source along the southeast (SE) vector. Nonlinear regression analysis of pollen deposition along the SE vector was used to estimate that 99.9% of shed pollen would be deposited within 154.4 m of the so

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Khan, Irum, Muhammad Kashif Naeem, Armghan Shahzad, Zijin Zhang, Jing Chen, and Muhammad Sajjad. "Optimizing Wheat Pollen Preservation for Enhanced Viability and In Vitro Germination." Agronomy 14, no. 1 (2024): 201. http://dx.doi.org/10.3390/agronomy14010201.

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Wheat pollen, which is characterized by its short lifespan, exhibits rapid germination after anthesis. The preservation of wheat pollen is contingent upon environmental factors including temperature, relative humidity, light, and wind. The aim is to explicate the process for efficiently storing wheat pollen, particularly with regard to breeding. The short longevity of wheat pollen grains renders it impractical to conduct tests for pollen viability and in vitro germination on a large scale. Herein, the impact of storage temperatures and duration was assessed on pollen viability and in vitro ger

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Fariroh, Indri, and Riza Yuli Rusdiana. "METHOD FOR DRYING MAIZE POLLEN." Jurnal Penelitian Pertanian Terapan 20, no. 2 (2020): 98–106. http://dx.doi.org/10.25181/jppt.v20i2.1583.

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Pollen managements consisted of suitable harvest timing, pollen drying to a certain misture content, pollen viability testing, and pollen storage. Maize pollen was sensitive to desiccation. This experiment was aimed to determine preservation technique in reducing pollen water content while maintaining its viability. Maize variety which used in this research was BIMA 3 as male line hybrid maize. Pollen viability testing used potassium iodide (KI) 1%. This experiment used randomized block design with 3 factors and 2 replicates, i.e. boron (H3BO3) fertilizers (0 and 3 kg ha-1 ), drying techniques

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Konwar, Surajeet, Dharitree Deka, Aandrisha Borthakur, and Bijoy Neog. "A Study on Pollen Morphology and Viability of Certain Members of Cucurbitaceae Family." South Asian Journal of Experimental Biology 14, no. 1 (2024): 21–30. http://dx.doi.org/10.38150/sajeb.14(1).p21-30.

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Characterizing the shape of pollen is crucial for plant breeding, taxonomy, and conservation, and studies on pollen viability can help breeding initiatives. This study examined the male fertility and pollen morphology of six Cucurbitaceae species with potential for both economic and nutritional benefit. Pollen grains were acetolyzed for morphological study, and a light compound microscope and an LEICA digital microscope were used to determine the exine stratification as well as other characteristics of pollen grains. The Cucurbitaceae family is eurypalynous, as evidenced by the noticeable diff

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Kusumastuti, Hamiddah Intan, Eny Widajati, and Endah Retno Palupi. "Cytological Deterioration of Bitter Gourd (Momordica charantia L.) Pollen During Storage and Its Impact on Effectiveness for Seed Production." Journal of Tropical Crop Science 12, no. 02 (2025): 303–13. https://doi.org/10.29244/jtcs.12.02.303-313.

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Pollen storage plays an important role in the hybrid seed production of bitter gourd, but a primary challenge is the rapid decline in pollen viability. This research aimed to investigate the mechanisms of bitter gourd pollen deterioration during storage and to assess the effectiveness of stored pollen for hybrid seed production. The study was conducted at the Leuwikopo Research Station, IPB University, from September 2018 to June 2021. Bitter gourd pollen was collected at anthesis and stored in a deep freezer at -21±3ºC. Pollen viability was observed at 0, 2, 4, 6, 8, 10, and 12 weeks after st

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Reid, Scott, Judy Harrington, and Harrison Hughes. "090 Pollen Viability in Inland Saltgrass." HortScience 34, no. 3 (1999): 457A—457. http://dx.doi.org/10.21273/hortsci.34.3.457a.

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Distichlis spicata var. stricta (Torrey) Beetle is a native grass that tolerates salt, high pH, and some heavy metals. It has been proposed for use in several challenging environments, including mine spoils and salt-impacted areas of golf courses. But, its widespread use has been hindered by several factors, one of which is poor seed set. Because chromosome numbers are variable and some genotypes are aneuploids, there was concern that pollen viability in some genotypes was low. Pollen from several genotypes failed to germinate in vitro on four artificial media prepared with various levels of o

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S, PAVITHRA, DHANANJAYA M. V, SUJATHA A. NAIR, et al. "Studies on pollen viability in Jasminum spp." Indian Journal of Agricultural Sciences 88, no. 12 (2018): 1864–68. http://dx.doi.org/10.56093/ijas.v88i12.85438.

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In vitro studies on pollen viability of six jasmine species, viz. Jasminum sambac, J. auriculatum, J. grandiflorum, J. multiflorum, J. flexile and J. malabaricum, was conducted at ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru during 2016-17. Results revealed that species Jasminum malabaricum recorded highest pollen viability (96.57%) as well as pollen germination (91.58%) followed by J. flexile (84.92% and 81.86%, respectively) and J. auriculatum (71.32% and 71.08%, respectively) under in vitro condition. The species J. sambac significantly recorded lowest

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Zeki, Kara, Mohammednoori Fakhar Mohammed Salah, and Yazar Kevser. "Pollen Characteristics of Diploid and Tetraploid Grape Cultivars." Global Journal of Fertility and Research 9, no. 1 (2024): 001–7. http://dx.doi.org/10.17352/gjfr.000025.

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For a successful grapevine breeding program, pollen viability, germination ability, and cultivar compatibility information are of vital importance. In this study, pollen viability, germination rates, pollen tube lengths, and pollen tube diameters of three grape cultivars [Kyoho (4n), Ekşi Kara (2n), and Gök Üzüm (2n)] were investigated. Pollen viability rates were determined using the IKI (iodine potassium iodide) staining test. In vitro, pollen germination rates were determined using a semi-solid medium containing 20% sucrose and 1% agar. As a result, significant differences were detected i

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Monteiro, Carlos Eduardo da Silva, Telma Nair Santana Pereira, and Karina Pereira de Campos. "Reproductive characterization of interspecific hybrids among Capsicum species." Crop Breeding and Applied Biotechnology 11, no. 3 (2011): 241–49. http://dx.doi.org/10.1590/s1984-70332011000300006.

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The objective of this study was the reproductive characterization of Capsicum accessions as well as of interspecific hybrids, based on pollen viability. Hybrids were obtained between Capsicum species. Pollen viability was high in most accessions, indicating that meiosis is normal, resulting in viable pollen grains. The pollen viability of species C. pubescens was the lowest (27 %). The interspecific hybrids had varying degrees of pollen viability, from fertile combinations (C. chinense x C. frutescens and C. annuum x C. baccatum) to male sterile combinations. Pollen viability also varied withi

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Đorđević, Milena, Tatjana Vujović, Radosav Cerović, et al. "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods." Horticulturae 8, no. 7 (2022): 616. http://dx.doi.org/10.3390/horticulturae8070616.

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A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196 °C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pol

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Deng, Zhanao, and Brent K. Harbaugh. "Technique for In Vitro Pollen Germination and Short-term Pollen Storage in Caladium." HortScience 39, no. 2 (2004): 365–67. http://dx.doi.org/10.21273/hortsci.39.2.365.

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The sporadic nature of inflorescence production and flower protogyny in caladium (Caladium ×hortulanum Birdsey) makes it desirable to store pollen and to rapidly assess its viability for cross-pollinations in breeding programs. This study was conducted to develop a procedure to determine caladium pollen viability and to use that procedure to evaluate the effect of short-term storage conditions on pollen viability. The sucrose level in the culture medium was found to have a significant impact on the in vitro germination of caladium pollen; a concentration of 6.8% was determined to be optimal fo

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Beyhan, N., and U. Serdar. "Assessment of pollen viability and germinability in some European chestnut genotypes (Castanea sativa L.)." Horticultural Science 35, No. 4 (2008): 171–78. http://dx.doi.org/10.17221/23/2008-hortsci.

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Pollen viability and germinability in some European chestnut genotypes was assessed in this study. In 10 chestnut genotypes, percentages of pollen viability were generally high and often around or over 80%. The pollen germination percentages of the genotypes were significantly affected by media sucrose concentrations. At optimum sucrose concentrations pollen germination percentages varied between 21.97 and 43.68% in 2004, 3.95 and 31.97% in 2005 and 6.79 and 31.03% in 2006, across all genotypes. The highest pollen germination percentage was obtained from 10% sucrose concentration in all years.

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McGregor, Cecilia E., and Vickie Waters. "Pollen Viability of F1 Hybrids between Watermelon Cultivars and Disease-resistant, Infraspecific Crop Wild Relatives." HortScience 48, no. 12 (2013): 1428–32. http://dx.doi.org/10.21273/hortsci.48.12.1428.

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Crop wild relatives (CWRs) are important sources of variation for domesticated crops like watermelon (Citrullus lanatus) where cultivated varieties have a very narrow genetic base. The use of CWRs in plant breeding can be hampered by low fertility, chromosomal rearrangements, marker distortion, and linkage drag in the progeny. Pollen viability can be a quick and easy way to estimate male fertility, which can be a cause of marker distortion and an indicator of chromosomal rearrangements. Pollen viability was determined for F1 hybrids between cultivars and resistant citron and egusi types and th

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Corrêa, Bruno Jan Schramm, Alexandra Cristina Schatz Sá, Luciana Magda de Oliveira, Adelar Mantovani, and Roseli Lopes da Costa Bortoluzzi. "Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen." Revista de Ciências Agroveterinárias 23, no. 2 (2024): 203–10. http://dx.doi.org/10.5965/223811712322024203.

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This study evaluated aspects of the pollen viability of the species Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae), both native trees with ornamental, medicinal and industrial potential. The study used different concentrations of sucrose to determine pollen viability in vitro and through colorimetric tests with specific dyes (Lugol, carmine acetic, and tetrazolium chloride – TTC – 0.1 and 0.05%). The two species also had their pollen stored for 30 and 90 days in freezers, refrigerators, and natural environments to check the best method for preserving th

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Kovács, G. "Estimation of pollen viability in einkorn (Triticum monococcum ssp. monococcum) accessions of different geographical origin." Acta Agronomica Hungarica 51, no. 4 (2003): 405–11. http://dx.doi.org/10.1556/aagr.51.2003.4.5.

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In the present experiment the pollen viability of einkorn accessions of different origin was tested using four vital dyes (TTC, Baker's procedure, MTT and FDA) to determine the potential of the dyes to differentiate fresh living pollen from pollen heated for 12 hours at 80ºC (killed pollen). It was found that two of the four dyes previously employed to determine pollen viability also stained killed pollen in the case of several einkorn accessions, while FDA and MTT did not. It is thus suggested that the two latter should be used to test einkorn pollen viability, since they do not normally stai

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Radovic, Aleksandar, Dragan Nikolic, Dragan Milatovic, Vera Rakonjac, and Ivana Bakic. "POLLEN VIABILITY IN QUINCE CULTIVARS." Journal of Agricultural, Food and Environmental Sciences 72, no. 1 (2018): 68–71. http://dx.doi.org/10.55302/jafes18721068r.

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Cohen, E., U. Lavi, and P. Spiegel-Roy. "Papaya pollen viability and storage." Scientia Horticulturae 40, no. 4 (1989): 317–24. http://dx.doi.org/10.1016/0304-4238(89)90105-2.

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Sparks, Darrell, and I. E. Yates. "Pecan Pollen Stored Over a Decade Retains Viability." HortScience 37, no. 1 (2002): 176–77. http://dx.doi.org/10.21273/hortsci.37.1.176.

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In vitro germination of freshly collected pollen and pollen stored 1, 10, 11, 12, and 13 years in liquid nitrogen was examined for `Desirable' pecan [Carya illinoinensis (Wangenh.) C. Koch]. Viability of pollen stored in liquid nitrogen for 1, 10, 11, 12, and 13 years was not diminished in comparison to that of fresh pollen. Morphology of stored pollen grains and the germ tube was normal. Thus, liquid nitrogen may offer a means of haploid preservation of pecan.

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