Journal articles: 'Polyl'

1

Ginsburg, Isaac, Ruth Borinski, Milu Sadovnic, Yael Eilam, and Kim Rainsford. "Polyl-histidine." Inflammation 11, no. 3 (September 1987): 253–77. http://dx.doi.org/10.1007/bf00915832.

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Oprisanu, Alexandra, Ioana Georgiana Lazar, Maria Daniela Pop, Eleonora Mihaela Ungureanu, Raluca Isopescu, and Liviu Birzan. "Polyazulenes Based Materials for Heavy Metal Ions Detection." Revista de Chimie 68, no. 10 (November 15, 2017): 2215–18. http://dx.doi.org/10.37358/rc.17.10.5858.

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Azulene is a special monomer used to functionalize electrodes due to its high polarizability, because it makes possible spontaneous electron drift from the five-membered ring to the seven-membered ring. Our study concerns the electrochemical characterization by cyclic voltammetry, differential pulse-voltammetry and rotating disk electrode voltammetry of a new azulene monomer, 4-(5-isopropyl-3,8-dimethylazulen-1-yl)-2,6-bis((E)-2-(thiophen-2-yl)vinyl) pyridine (L). It has been used to obtain complexing modified electrodes by electrochemical polymerization. PolyL films modified electrodes have been characterized by cyclic voltammetry in ferrocene probe solution. The complexing properties of polyL based functional materials have been investigated towards heavy metals (Pb, Cd Hg, Cu) by preconcentration � anodic stripping technique.

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Lazar, Ioana Georgiana, Elena Diacu, George Octavian Buica, Eleonora Mihaela Ungureanu, Georgiana Luiza Arnold, and Liviu Birzan. "The Heavy Metals Sensing Based on 2,6-Bis(-2-(Thiophen-3-yl)Vinyl)-4-(4,6,8-Trimethylazulen-1-yl)Pyrylium Modified Electrodes." Revista de Chimie 68, no. 11 (December 15, 2017): 2509–13. http://dx.doi.org/10.37358/rc.17.11.5917.

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This paper focuses on electrochemical characterization of new azulene compound 2,6-bis((E)-2-(thiophen-3-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyrylium (L) by cyclic voltammetry (CV), differential pulse voltammetry (DPV), rotating disk electrode voltammetry (RDE) on glassy carbon disk working electrodes. The character of redox processes was established by CV and RDE using increasing scan rates at different scan domains. PolyL modified electrodes have been prepared by scanning or controlled potential electrolysis (CPE). The complexing properties of L and polyL have been investigated towards detection of heavy metal ions by anodic stripping technique. The voltammetric curves present well defined peaks for Pb2+, Cd2+, Cu2+ and Hg2+, which can be used as analytical signals for their determination. The performances of the modified electrodes obtained at different potentials and charges have been evaluated. The best results have been obtained for Pb2+ and Cd2+ with the achievement of detection limits of 10-7 M.

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Pop, Maria Daniela, Oana Brincoveanu, Mihaela Cristea, George Octavian Buica, Marius Enachescu, and Eleonora Mihaela Ungureanu. "AFM and SEM Characterization of Chemically Modified Electrodes Based on 5-[(azulen-1-yl) methylene]-2-thioxothiazolidin-4-one." Revista de Chimie 68, no. 12 (January 15, 2018): 2799–803. http://dx.doi.org/10.37358/rc.17.12.5981.

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Preparation and microscopy characterization of polymer modified glassy carbon electrodes based on (5-[(azulen-1-yl) methylene]-2-thioxothiazolidin-4-one (L) were reported. Atomic Force Microscopy was used to investigate the morphological and mechanical properties of the deposited polyL films onto glassy carbon. The topography images of the analyzed samples exhibited the presence of some columnar shape features onto the layer surfaces. The surface roughness of the layers deposited at constant charge calculated from topography images, increased with the more positive applied potential for controlled potential electrolysis. At different charges, the roughness parameter showed the same behavior for the layers obtained applying a constant potential without having a noticeable influence on the adhesion properties on the substrate. Analysis using scanning electron microscopy shows a relatively uniform surface arrangement of the polymer and the presence of some clusters which are disturbing the planarity. PolyL chemically modified electrodes have been used for heavy metal ions detection with best results for lead.

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Li, Qing, Yiling Zhang, Jingbing Li, Hao Wang, Hui Lu, Honghao Fan, and Gang Zheng. "Stimulatory effects of poly-para-dioxanone, poly L-lactic acid, polycaprolactone, and poly(lactic-co-glycolic acid)/PLLA in rats." Materials Express 9, no. 8 (November 1, 2019): 962–69. http://dx.doi.org/10.1166/mex.2019.1582.

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Numerous fillers are increasingly used for augmenting volume loss and ameliorating facial wrinkles. Collagen stimulants are the most recent next-generation dermal fillers capable of inducing neocollagenesis. To investigate biophysical characteristics, the safety and efficacy of the newly developed combination, poly(L-lactide) or polyl-lactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA), were compared with those of poly-para-dioxanone (PPDO), poly-l-lactic acid (PLLA), and polycaprolactone (PCL) fillers. PPDO, PLLA, PCL, or PLGA/PLLA was injected into the dorsal skin of 8-week-old rats. Tissue samples were obtained 0, 2, and 4 weeks post treatment for Sirius Red-staining, polarized light microscopy, enzyme-linked immunosorbent assay (ELISA), real-time reverse transcription PCR (qRT-PCR), and western blotting. The MagBeads Total RNA Extraction Kit was used to isolate total cell RNA. The PLGA/PLLA filler demonstrated similar neocollagenesis and inflammatory response as other collagen stimulants, and showed better biodegradability than PPDO, PLLA and PCL fillers. Our data suggest that the newly developed collagen-stimulating PLGA/PLLA filler may be a safe and effective option for correcting volume loss and rejuvenating photoaging skin.

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Bello, Jake, Edward N. Granados, Steven Lewinski, Helene R. Bello, and Thomas Trueheart. "Methylated Polyl(L-lysine): Conformational Effects and Interactions with Polynucleotides." Journal of Biomolecular Structure and Dynamics 2, no. 5 (February 1985): 899–913. http://dx.doi.org/10.1080/07391102.1985.10507608.

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7

Tonegawa, Toru, Toshiyuki Ikoma, Guo Ping Chen, and M. Tanaka. "Control of Protein Initial Burst from Zinc-Hydroxyapatite / Poly(L-Lactide) System." Key Engineering Materials 330-332 (February 2007): 857–60. http://dx.doi.org/10.4028/www.scientific.net/kem.330-332.857.

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Control of protein release without the initial burst from zinc containing hydroxyapatite porous microparticles (Zn-HAp) was investigated with a novel formulation method. The formulation method was a coat of polyL(L-lactide; PLA) on the microparticle with nano-thickness, which obviously suppressed the initial burst of protein release compared with the microparticles without the formulation, the coat of PLA. The HAp/PLA microparticle with 1-20μm of the size distribution was available for the drug delivery carrier of proteins without the degradation.

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Khajeheian, Mohammad B., and Ari Rosling. "Preparation and characterization of linear and star-shaped polyL-lactide blends." Journal of Applied Polymer Science 133, no. 2 (April 11, 2015): n/a. http://dx.doi.org/10.1002/app.42231.

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9

Bugean, Ioana-Georgiana, Adinuta Paun, Elena Diacu, Mihaela Cristea, Liviu Birzan, and Eleonora-Mihaela Ungureanu. "Chemically Modified Electrodes Based on 4-(azulen-1-yl)-2,6-bis ((e)-2-(thiophen-2-yl)vinyl) Pyrylium Perchlorate." Revista de Chimie 71, no. 9 (September 5, 2020): 113–24. http://dx.doi.org/10.37358/rc.20.9.8322.

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The electrochemical behavior of 4-(azulen-1-yl)-2,6-bis((E)-2-(thiophen-2-yl)vinyl) pyrylium perchlorate (L) was studied by cyclic voltammetry, differential pulse voltammetry, and rotating disk electrode voltammetry. The electrochemical polymerization of L was studied. Modified electrodes covered by polyL films were obtained by successive scanning or by controlled potential electrolysis (CPE) at different charges and potentials. The new electrodes obtained by CPE were applied for sensing the following heavy metals ions: Cu(II), Pb(II), Cd(II), Hg(II). The best results have been registered for Pb(II) ion with a detection limit of 10-8 M.

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10

Nakamura, Kosuke, Takahiro Ohtsuki, Haruyo Mori, Hiroo Hoshino, Ariful Hoque, Atsushi Oue, Fumie Kano, et al. "Novel anti-HIV-1 activity produced by conjugating unsulfated dextran with polyl-lysine." Antiviral Research 94, no. 1 (April 2012): 89–97. http://dx.doi.org/10.1016/j.antiviral.2012.02.011.

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11

Xiao, Yin, Hui Peng, Xueli Mao, Andrew K. Whittaker, and Ross Crawford. "Novel Synthetic Bio-Mimic Polymers for Cell Delivery." Advanced Materials Research 32 (February 2008): 215–22. http://dx.doi.org/10.4028/www.scientific.net/amr.32.215.

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Cell-based therapy is one of the major potential therapeutic strategies for cardiovascular, neuronal and degenerative diseases in recent years. The aims of this study is to develop a novel biomimic polymeric materials which will facilitate the delivery cells, control cell bioactivities and enhance the focal integration of graft cells with host tissues. We synthesized a novel tri-block copolymer, methoxy-terminated poly (ethylene glycol) (MPEG)-polyL-lactide (PLLA)-polylysine (PLL) via sequential polymerization of PLLA onto MPEG, followed by ring opening polymerization of PLL onto the functionalized chain end. The triblock copolymer (5%) was then mixed with high molecular weight PLLA (95%) to form cell-delivery membranes. The spectra of copolymers were determined by NMR and ATR-FTIR spectroscopy. Human osteoblasts were used for testing biocompatibility and initial cellular reaction. It was noted that no cytotoxicity was detectable in our synthesized copolymers. Compared with pure PLLA and diblock copolymers, the triblock copolymers showed significantly better cell adhesion and proliferation. Interestingly we identified that cellular activity (attachment, proliferation and differentiation) could be regulated by the molecular weight and composition of the triblock copolymers. In conclusion controllable synthetic copolymers can be designed and synthesized to modulate cellular function in facilitating tissue repair and regeneration.

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12

McConnon, Aili. "Investigating thermoelectric properties in conjugated polymer polyl thin films to achieve higher levels of performance." Scilight 2021, no. 38 (September 17, 2021): 381109. http://dx.doi.org/10.1063/10.0006478.

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13

Mishra, Brijeshkunvar J., Ankur Kaul, and Piyush Trivedi. "l-Cysteine conjugated polyl-lactide nanoparticles containing 5-fluorouracil: formulation, characterization, release and uptake by tissuesin vivo." Drug Delivery 22, no. 2 (February 14, 2014): 214–22. http://dx.doi.org/10.3109/10717544.2014.883117.

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14

Esmaeilzadeh, Jaleh, Hossein Nazemiyeh, Maryam Maghsoodi, and Farzaneh Lotfipour. "Evaluation of the Effect of Psyllium on the Viability of Lactobacillus Acidophilus in Alginate-Polyl Lysine Beads." Advanced Pharmaceutical Bulletin 6, no. 3 (September 25, 2016): 337–43. http://dx.doi.org/10.15171/apb.2016.045.

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15

Гриневич, А. А., И. С. Масулис, and Л. В. Якушевич. "МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ ПОВЕДЕНИЯ ТРАНСКРИПЦИОННОГО ПУЗЫРЯ В ПЛАЗМИДЕ PPF1 И ЕЕ МОДИФИКАЦИЯХ. СВЯЗЬ МЕЖДУ ЭНЕРГЕТИЧЕСКИМ ПРОФИЛЕМ ПЛАЗМИДЫ И НАПРАВЛЕНИЕМ ТРАНСКРИПЦИИ." Биофизика 66, no. 2 (2021): 248–58. http://dx.doi.org/10.31857/s0006302921020058.

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C использованием нелинейного уравнения синус-Гордона и его решения - кинка - моделировали и изучали динамику транскрипционного пузыря в плазмиде pPF1. На основе рассчитанного энергетического профиля плазмиды pPF1 и ее модификаций было показано, что минимальная потенциальная энергия формирования кинка или нуклеации транскрипционного пузыря соответствует области между генами белков Egfp и mCherry. Вставка в область между Egfp и mCherry однородных последовательностей показала, что кинк с большей вероятностью активируется в polyT или polyC по сравнению с polyA или polyG, что свидетельствует о зависимости нуклеации транскрипционного пузыря от физической массы оснований. В случае вставок в область между Egfp и mCherry небольших фрагментов природной последовательности Escherichia coli модель позволяет выявить нити ДНК с наибольшей вероятностью нуклеации транскрипционного пузыря и соответственно определить направление (в сторону гена Egfp или mCherry) транскрипции, что говорит о связи между направлением транскрипции и энергетическим профилем плазмиды.

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Zhu, Benwei, Limin Ning, Yucui Jiang, and Lin Ge. "Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine Bacterium Isoptericola halotolerans." Marine Drugs 16, no. 8 (July 31, 2018): 258. http://dx.doi.org/10.3390/md16080258.

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Alginate lyases are important tools to prepare oligosaccharides with various physiological activities by degrading alginate. Particularly, the bifunctional alginate lyase can efficiently hydrolyze the polysaccharide into oligosaccharides. Herein, we cloned and identified a novel bifunctional alginate lyase, AlyA, with a high activity and broad substrate specificity from bacterium Isoptericola halotolerans NJ-05 for oligosaccharides preparation. For further applications in industry, the enzyme has been characterized and its action mode has been also elucidated. It exhibited the highest activity (7984.82 U/mg) at pH 7.5 and 55 °C. Additionally, it possessed a broad substrate specificity, showing high activities towards not only polyM (polyβ-d-mannuronate) (7658.63 U/mg), but also polyG (poly α-l-guluronate) (8643.29 U/mg). Furthermore, the Km value of AlyA towards polyG (3.2 mM) was lower than that towards sodium alginate (5.6 mM) and polyM (6.7 mM). TLC (Thin Layer Chromatography) and ESI-MS (Electrospray Ionization Mass Spectrometry) were used to study the action mode of the enzyme, showing that it can hydrolyze the substrates in an endolytic manner to release a series of oligosaccharides such as disaccharide, trisaccharide, and tetrasaccharide. This study provided extended insights into the substrate recognition and degrading pattern of the alginate lyases, with a broad substrate specificity.

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Hearn, T. C., M. Arighi, and J. R. Field. "Investigation of Bioabsorbable Screw Usage for Longbone Fracture Repair in the Horse: Biomechanical Features." Veterinary and Comparative Orthopaedics and Traumatology 06, no. 01 (1993): 42–46. http://dx.doi.org/10.1055/s-0038-1633145.

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SummaryThe purpose of the study was to compare the biomechanical features of bioabsorbable, self-reinforced polyL-lactic acid (SR-PLLA) and stainless steel 4.5 mm ASIF screws. Paired extraction tests were conducted in matched third metacarpal bones and in a synthetic bone material composed of polyurethane foam. The tests were accomplished using a servohydraulic materials testing machine. From these tests the maximum value of tensile force, achieved during extraction, was recorded as the screw “holding strength‘.Comparison of holding strengths in horse cadaver bones revealed a significant difference (p = 0.0002) between screw types. The axial tensile strength of the SR-PLLA screws (878 Newtons) was much lower than that achieved by the ASIF screws (8513 N). Subsequent testing in synthetic material again reflected significant differences (p <0.0001) between the screw types. The results may be accounted for by differences in the thread-to-core ratio between the screw types. Other factors, such as elastic deformation of the bioabsorbable screw, may also contribute through differences in the mechanical interface between screw and media.

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Liao, Yu Bo, Yao Yong Meng, Hao Dong Lei, and Zhao Sheng Lai. "Raman Spectroscopic Study of Nucleobase Pairs and Mispairs - A Potential Method for Analysis of DNA Sequences." Advanced Materials Research 554-556 (July 2012): 1816–21. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1816.

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To exploit sensitive, accurate and convenient detection techniques for nucleic acid sequences can help to overcome the defects of current fluorescence detection method. Raman spectroscopy represents one family of methods that may offer such potential. In this paper, G•C base pairs and C•U mispairs were studied by Raman spectral characterization of single-strand homopolynucleotides, i.e. polyguanylic acid (PolyG), polycytidylic acid (PolyC) and polyuridylic acid (PolyU), and their equimolar complexes PolyG•PolyC and PolyC•PolyU in solution of 0.14 M Na+, pH7.0. Both of the complexes were found to adopt A type conformation. Helix formation between complementary polynucleotides led to marked and characterisctic changes in frequency and intensity of Raman bands of the biopolymers. Spectral changes were also observed upon the formation of C•U mispairs. According to the Raman data, in C•U mispairs the C2=O of U was engaged in a hydrogen bond to the N4H2group of cytosine, while the uracil C4=O group is free. Some of the rC residues transformed from C3-endo/anti to C2-endo/anti conformation due to base mispairing.The results suggest that Raman spectroscopy provides detailed and highly sensitive information about both the structure of nucleic acids and their base composition, and has great potential for analysis of DNA sequences.

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Skálová, Tereza, Jarmila Dušková, Jindřich Hašek, Petr Kolenko, Andrea Štěpánková, and Jan Dohnálek. "Alternative polymer precipitants for protein crystallization." Journal of Applied Crystallography 43, no. 4 (May 22, 2010): 737–42. http://dx.doi.org/10.1107/s0021889810014317.

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A set of 16 inexpensive and commercially available polymer precipitants were tested for protein crystallization. Eight of them were found suitable: polyethylene glycol dimethyl ether of molecular weight (MW) 500, 1000 and 2000; di[poly(ethylene glycol)] adipate, MW 900; poly(ethylene glycol-ran-propylene glycol), MW 2500 and 12000; poly(acrylic acid) sodium salt, MW 2100; and polyethylene glycol methyl ether methacrylate, MW 1100. Two new crystallization screens, PolyA and PolyB, were formulated using these eight polymers, each containing 96 solutions – four polymers in combination with 24 common salts and buffers, covering pH values from 4.5 to 9.0. The screens were tested on 29 proteins, 21 of which were crystallized. The tests confirmed the applicability of the eight polymers as precipitants for protein crystallization.

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Cheatham, Thomas E., Jayashree Srinivasan, David A. Case, and Peter A. Kollman. "Molecular Dynamics and Continuum Solvent Studies of the Stability of PolyG-PolyC and PolyA-PolyT DNA Duplexes in Solution." Journal of Biomolecular Structure and Dynamics 16, no. 2 (October 1998): 265–80. http://dx.doi.org/10.1080/07391102.1998.10508245.

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21

Urata, T., K. Arimori, and M. Nakano. "Modification of release rates of cyclosporin A from polyl(L-lactic acid) microspheres by fatty acid esters and in-vivo evaluation of the microspheres." Journal of Controlled Release 58, no. 2 (March 1999): 133–41. http://dx.doi.org/10.1016/s0168-3659(98)00146-1.

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22

Werner, Ralf, Paul-Martin Holterhus, Gerhard Binder, Hans-Peter Schwarz, Michel Morlot, Dagmar Struve, Christine Marschke, and Olaf Hiort. "The A645D Mutation in the Hinge Region of the Human Androgen Receptor (AR) Gene Modulates AR Activity, Depending on the Context of the Polymorphic Glutamine and Glycine Repeats." Journal of Clinical Endocrinology & Metabolism 91, no. 9 (September 1, 2006): 3515–20. http://dx.doi.org/10.1210/jc.2006-0372.

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Abstract Background: Sufficient androgen receptor (AR) activity is crucial for normal male sexual differentiation. Here we report on two unrelated 46, XY patients suffering from undervirilization and genital malformations. Both patients had a short polyglycine (polyG) repeat of 10 residues and a relatively long polyglutamine (polyQ) repeat of 28 and 30 residues within the transactivation domain of the AR. In addition, they also harbor a rare A645D substitution. Objective: We made a set of AR expression plasmid constructs with varying polyQ and polyG tract sizes in context with or without the A645D substitution and analyzed their in vitro transactivation capacity in transfected CHO cells. Results: We found that a short polyG repeat downmodulated AR activity to approximately 60–65% of the wild-type receptor. This effect was aggravated by A645D in context of a long polyQ repeat to less than 50% activity. In contrast, in the context of a short polyQ and a short polyG repeat, the A645D mutation rescues AR activity to almost wild-type levels, demonstrating a contradictory effect of this mutation, depending on the size of the polymorphic repeats. Conclusions: A combination of a short polyG repeat with a long polyQ repeat and an A645D substitution might contribute to the development of virilization disorders and explain the observed phenotypes of our patients as a form of androgen insensitivity. The whole recreation of AR sequence variations including individual polymorphic repeat sizes could unravel possible interference of mutations and variations on AR activity by in vitro transfection.

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Zhang, Fengchao, Zheng Fu, Luyao Tang, Zhelun Zhang, Feng Han, and Wengong Yu. "Biochemical Characterization of a Novel Exo-Type PL7 Alginate Lyase VsAly7D from Marine Vibrio sp. QY108." International Journal of Molecular Sciences 22, no. 16 (August 5, 2021): 8402. http://dx.doi.org/10.3390/ijms22168402.

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Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L-erythro-5-hexoseulose uronate (DEH) by a non-enzyme reaction, which is an important raw material for the preparation of bioethanol. In our research, a novel exo-type alginate lyase, VsAly7D, belonging to the PL7 family was isolated from marine bacterium Vibrio sp. QY108 and recombinantly expressed in Escherichia coli. The purified VsAly7D demonstrated the highest activity at 35 °C, whereas it still maintained 46.5% and 83.1% of its initial activity at 20 °C and 30 °C, respectively. In addition, VsAly7D exhibited the maximum activity under alkaline conditions (pH 8.0), with the simultaneously remaining stability between pH 8.0 and 10.0. Compared with other reported exo-type enzymes, VsAly7D could efficiently degrade alginate, poly-β-D-mannuronate (polyM) and poly-α-L-guluronate (polyG) with highest specific activities (663.0 U/mg, 913.6 U/mg and 894.4 U/mg, respectively). These results showed that recombinant VsAly7D is a suitable tool enzyme for unsaturated alginate monosaccharide preparation and holds great promise for producing bioethanol from brown algae.

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Wise, Nicola. "Expression of concern: Synthesis of poly(ethylene-co-acrylic acid) via a tandem hydrocarboxylation/hydrogenation of poly(butadiene)." Polymer Chemistry 6, no. 2 (2015): 330. http://dx.doi.org/10.1039/c4py90087a.

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Expression of concern for ‘Synthesis of poly(ethylene-co-acrylic acid) via a tandem hydrocarboxylation/hydrogenation of poly(butadiene)’ by Kelly M. Wiggins and Christopher W. Bielawski, Polym. Chem., 2013, 4, 2239–2245.

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Moshe, Hen, Yuval Davizon, Maya Menaker Raskin, and Alejandro Sosnik. "Novel poly(vinyl alcohol)-based amphiphilic nanogels by non-covalent boric acid crosslinking of polymeric micelles." Biomater. Sci. 5, no. 11 (2017): 2295–309. http://dx.doi.org/10.1039/c7bm00675f.

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In this work, we report a new type of poly(vinyl alcohol)-g-poly(N-isopropylacrylamide) (PVA-g-PNiPAAm) amphiphilic nanogel produced by the non-covalent crosslinking of PVA polyol domains in preformed polymeric micelles with boric acid.

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Yang, Jin, Dandan Cui, Diwen Chen, Wenkang Chen, Shuo Ma, and Hong Shen. "Purification and Characterization of a Novel Endolytic Alginate Lyase from Microbulbifer sp. SH-1 and Its Agricultural Application." Marine Drugs 18, no. 4 (March 31, 2020): 184. http://dx.doi.org/10.3390/md18040184.

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Alginate, an important acidic polysaccharide in marine multicellular algae, has attracted attention as a promising biomass resource for the production of medical and agricultural chemicals. Alginate lyase is critical for saccharification and utilization of alginate. Discovering appropriate and efficient enzymes for depolymerizing alginate into fermentable fractions plays a vital role in alginate commercial exploitation. Herein, a unique alginate lyase, AlgSH7, belonging to polysaccharide lyase 7 family is purified and characterized from an alginate-utilizing bacterium Microbulbifer sp. SH-1. The purified AlgSH7 shows a specific activity of 12,908.26 U/mg, and its molecular weight is approximately 66.4 kDa. The optimal temperature and pH of AlgSH7 are 40 °C and pH 9.0, respectively. The enzyme exhibits stability at temperatures below 30 °C and within an extensive pH range of 5.0–9.0. Metal ions including Na+, K+, Al3+, and Fe3+ considerably enhance the activity of the enzyme. AlgSH7 displays a preference for poly-mannuronic acid (polyM) and a very low activity towards poly-guluronic acid (polyG). TLC and ESI-MS analysis indicated that the enzymatic hydrolysates mainly include disaccharides, trisaccharides, and tetrasaccharides. Noteworthy, the alginate oligosaccharides (AOS) prepared by AlgSH7 have an eliciting activity against chilling stress in Chinese flowering cabbage (Brassica parachinensis L.). These results suggest that AlgSH7 has a great potential to design an effective process for the production of alginate oligomers for agricultural applications.

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Bruggeman, Joost P., Berend-Jan de Bruin, Christopher J. Bettinger, and Robert Langer. "Biodegradable poly(polyol sebacate) polymers." Biomaterials 29, no. 36 (December 2008): 4726–35. http://dx.doi.org/10.1016/j.biomaterials.2008.08.037.

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Sauty, Nicolas F., Lucas Caire da Silva, Caitlyn Gallagher, Robert Graf, and Kenneth B. Wagener. "Correction: Unveiling the hyperbolic thermal behaviour of poly(p-phenylene alkylene)s." Polymer Chemistry 7, no. 2 (2016): 502. http://dx.doi.org/10.1039/c5py90201k.

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Koo, J. M., S. Y. Hwang, W. J. Yoon, Y. Lee, S. H. Kim, and S. S. Im. "Correction: Structural and thermal properties of poly(1,4-cyclohexane dimethylene terephthalate) containing isosorbide." Polymer Chemistry 6, no. 39 (2015): 7069. http://dx.doi.org/10.1039/c5py90144h.

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Smith, Stephanie A., Julie N. Collins, Sharon H. Choi, and James H. Morrissey. "Novel Polyphosphate-Binding Proteins in Human Plasma and Platelet Releasates." Blood 118, no. 21 (November 18, 2011): 2270. http://dx.doi.org/10.1182/blood.v118.21.2270.2270.

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Abstract Abstract 2270 Introduction: Inorganic polyphosphates (polyP) are negatively charged, linear phosphate polymers found throughout biology. PolyP is secreted in substantial amounts by activated human platelets and it also accumulates in many infectious microorganisms. We have shown that polyP is a potent hemostatic regulator, acting at multiple points in the clotting cascade; in particular, polyP triggers the contact pathway, enhances FXI activation by thrombin, accelerates factor V activation, and modulates fibrin clot structure. Since there may be other, as-yet undiscovered (patho)physiologic roles for polyP, we undertook a study to identify the major polyP-binding proteins in human plasma and in releasates from activated human platelets. Methods: PolyP was end-labeled by biotinylation, then bound to streptavidin coated onto magnetic beads (Dynabeads), polystyrene microtiter plates, or surface plasmon resonance sensorchips. PolyP-Dynabeads were exposed to plasma or to releasates from platelets activated by TRAP; the beads were then washed repeatedly, and polyP-binding proteins were eluted with 1 M LiCl. Eluted proteins were separated by 2-D gel electrophoresis and were identified by mass spectrometry. Some additional proteins known or suspected to bind to polyP were also analyzed in parallel. Binding affinities for selected proteins were quantified by microtiter plate assays or by surface plasmon resonance approaches. Results: Candidate poly-P binding proteins identified in plasma pull-downs included parvulin, factor H, complement C1q, complement C1q tumor necrosis factor-related protein, C4 binding protein, and apolipoprotein E. Candidate poly-P binding proteins identified in pull-downs of platelet-releasate included platelet factor 4, multimerin, thrombospondin, and cyclophilin. High affinity binding to polyP was also quantified for thrombin, FXI, FXIa, kallikrien, histones and the isolated polyphosphate-binding domain of E. coli exopolyphosphatase. Conclusions: PolyP binds with high affinity to certain proteins that participate in the coagulation cascade as well as several additional proteins. Some of these are involved in reactions known to be modulated by polyP, but several have yet to be evaluated. Further investigation is indicated into potential roles for polyP in modulation of the complement cascade, prolyl isomerase activity, angiogenesis, and platelet responses. Disclosures: Smith: University of Illinois: Patents & Royalties. Choi:University of Illinois: Patents & Royalties. Morrissey:University of Illinois: Patents & Royalties.

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Somisetti, Varaprasad, Shaik Allauddin, Ramanuj Narayan, and K. V. S. N. Raju. "Synthesis of a novel glycerol based B3-type monomer and its application in hyperbranched polyester urethane–urea coatings." RSC Advances 5, no. 90 (2015): 74003–11. http://dx.doi.org/10.1039/c5ra11712g.

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Crisan, Daniel N., Oliver Creese, Ranadeb Ball, Jose Luis Brioso, Ben Martyn, Javier Montenegro, and Francisco Fernandez-Trillo. "Correction: Poly(acryloyl hydrazide), a versatile scaffold for the preparation of functional polymers: synthesis and post-polymerisation modification." Polymer Chemistry 10, no. 8 (2019): 1026. http://dx.doi.org/10.1039/c9py90016k.

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Correction for ‘Poly(acryloyl hydrazide), a versatile scaffold for the preparation of functional polymers: synthesis and post-polymerisation modification’ by Daniel N. Crisan et al., Polym. Chem., 2017, 8, 4576–4584.

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Fujimura, Atsushi, Seiji Yasui, Kazuyo Igawa, Ai Ueda, Kaori Watanabe, Tadashi Hanafusa, Yasuaki Ichikawa, et al. "In Vitro Studies to Define the Cell-Surface and Intracellular Targets of Polyarginine-Conjugated Sodium Borocaptate as a Potential Delivery Agent for Boron Neutron Capture Therapy." Cells 9, no. 10 (September 23, 2020): 2149. http://dx.doi.org/10.3390/cells9102149.

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Boron neutron capture therapy (BNCT) requires pharmaceutical innovations and molecular-based evidence of effectiveness to become a standard cancer therapeutic in the future. Recently, in Japan, 4-borono-L-phenylalanine (BPA) was approved as a boron agent for BNCT against head and neck (H&N) cancers. H&N cancer appears to be a suitable target for BPA-BNCT, because the expression levels of L-type amino acid transporter 1 (LAT1), one of the amino acid transporters responsible for BPA uptake, are elevated in most cases of H&N cancer. However, in other types of cancer including malignant brain tumors, LAT1 is not always highly expressed. To expand the possibility of BNCT for these cases, we previously developed poly-arginine peptide (polyR)-conjugated mercaptoundecahydrododecaborate (BSH). PolyR confers the cell membrane permeability and tumor selectivity of BSH. However, the molecular determinants for the properties are not fully understood. In this present study, we have identified the cluster of differentiation 44 (CD44) protein and translational machinery proteins as a major cell surface target and intracellular targets of BSH-polyR, respectively. CD44, also known as a stem cell-associated maker in various types of cancer, is required for the cellular uptake of polyR-conjugated molecules. We showed that BSH-polyR was predominantly delivered to a CD44High cell population of cancer cells. Once delivered, BSH-polyR interacted with the translational machinery components, including the initiation factors, termination factors, and poly(A)-biding protein (PABP). As a proof of principle, we performed BSH-polyR-based BNCT against glioma stem-like cells and revealed that BSH-polyR successfully induced BNCT-dependent cell death specifically in CD44High cells. Bioinformatics analysis indicated that BSH-polyR would be suitable for certain types of malignant tumors. Our results shed light on the biochemical properties of BSH-polyR, which may further contribute to the therapeutic optimization of BSH-BNCT in the future.

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Grimm, Oliver, Sarina C. Maßmann, and Felix H. Schacher. "Correction: Synthesis and solution behaviour of dual light- and temperature-responsive poly(triethylene glycol-co-spiropyran) copolymers and block copolymers." Polymer Chemistry 10, no. 29 (2019): 4067–68. http://dx.doi.org/10.1039/c9py90101a.

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Correction for ‘Synthesis and solution behaviour of dual light- and temperature-responsive poly(triethylene glycol-co-spiropyran) copolymers and block copolymers’ by Oliver Grimm et al., Polym. Chem., 2019, 10, 2674–2685.

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Cao, Yi, Min Liu, Guangyue Zu, Ye Kuang, Xiaoyan Tong, Dangsheng Xiong, and Renjun Pei. "Correction: Hyperbranched poly(glycerol) as a T1 contrast agent for tumor-targeted magnetic resonance imaging in vivo." Polymer Chemistry 8, no. 6 (2017): 1114. http://dx.doi.org/10.1039/c7py90018j.

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Ain, Nurul H., Maznee TI Tuan Noor, Mohd Azmil Mohd Noor, Adnan Srihanum, Kosheela PP Devi, Norhisham S. Mohd, Norhayati Mohdnoor, et al. "Structure–property performance of natural palm olein polyol in the viscoelastic polyurethane foam." Journal of Cellular Plastics 53, no. 1 (July 28, 2016): 65–81. http://dx.doi.org/10.1177/0021955x16639031.

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Structure–property behavior of the palm olein-based natural oil polyol (E-135 NOP) was investigated in viscoelastic “memory” foams. In a model viscoelastic foam formulation, the E-135 NOP with pendant hydroxyls was used as a drop-in replacement for the well-defined model polyether polyol with terminal hydroxyls, Poly-G® 76-120. Both polyols have comparable equivalent weight and concentrations of primary and secondary hydroxyls. The data showed that replacing Poly-G® 76-120 polyether polyol with the E-135 NOP did not significantly impact the foaming reactivity. Increasing the E-135 NOP concentration in the VE foams increased the average foam cell size while maintaining the open cell structure. Aging properties of the VE foams were mostly unaffected by the replacement of the Poly-G® 76-120 with the E-135 NOP. Furthermore, addition of E-135 had no impact on foam density; however, it increased the support factor of the viscoelastic foams. Differential scanning calorimetry, dynamic mechanical analyzer, and Fourier transform infrared spectroscopy analyses indicate less defined morphological separation of hard and soft segments in the viscoelastic foams with higher concentration of E-135 NOP. Overall, the results demonstrated the feasibility that natural oil polyols can be used in viscoelastic polyurethane foams to replace a significant portion of the polyether polyols with comparable equivalent weights and concentrations of primary and secondary hydroxyls. In future, the feasibility study of E-135 NOP as a drop-in replacement of combination polyether polyols in viscoelastic foams formulation will be conducted. Furthermore, the effect of palm olein-based natural oil polyol in high resilience foam will be evaluated.

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Kuznetsov, Vyacheslav A., Maria S. Lavlinskaya, and Andrey V. Sorokin. "Синтез графт-сополимеров натриевой соли карбоксиметилцеллюлозы с N-винилимидазолом и исследование свойств их водных дисперсий." Kondensirovannye sredy i mezhfaznye granitsy = Condensed Matter and Interphases 21, no. 2 (June 14, 2019): 231–39. http://dx.doi.org/10.17308/kcmf.2019.21/761.

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Радикальной полимеризацией в присутствии пероксида водорода получены графт-сополимеры натриевой соли карбоксиметилцеллюлозы и N-винилимидазола. Структура полученных продуктов подтверждена методом ИК-спектроскопии. Методами просвечивающей электронной микроскопии, динамического светорассеяния и лазерного допплеровского микроэлектрофореза установлено, что частицы дисперсий сополимеров имеют несферическую форму и отрицательный электрокинетический потенциал. Изучено влияние присутствие хлорида натрия на гидродинамический радиус частиц полимеров REFERENCES Madruga E. From classical to living/controlled statistical free-radical copolymerization // Prog. Polym. Sci., 2002, v. 27, pp. 1879–1924. https://doi.org/10.1016/S0079-6700(02)00023-0 Barouti G., Jaffredo C. G., Guillaume S. M. Advances in drug delivery systems based on synthetic poly(hydroxybutyrate) (co)polymers // Polym. Sci., 2017, v. 73, pp. 1–31. https://doi.org/10.1016/j.progpolymsci. 2017.05.002 Kabanov A. V., Vinogradov S. V. Nanogels as pharmaceutical carriers: fi nite networks of infi nite capabilities // Chem. Int. Ed., 2009, v. 48, pp. 5418–5429. https://doi.org/10.1002/anie.200900441 Oh J. K., Drumright R., Siegwart D., Matyjaszewski K. The development of microgels/nanogels for drug delivery applications // Polym. Sci., 2008, v. 33, pp. 448–477. https://doi.org/10.1016/j.progpolymsci.2008.01.002 Kuznetsov V. A., Lavlinskaya M. S., Ostankova I. V. Synthesis of N-vinylformamide and 1-vinyl-(1-methacryloyl)-3,5-dimethylpyrazole copolymers and their extraction ability in relation to histidine in watersalt media // Bull., 2018, v. 75, pp. 1237–1251. https://doi.org/10.1007/s00289-017-2091-2 Bhattacharya A., Misra B. Grafting: a versatile means to modify polymersTechniques, factors and applications // Polym. Sci., 2004, v. 29, pp. 767–814. https://doi.org/10.1016/j. progpolymsci.2004.05.002 Rasoulzadeh M., Namaz H. Carboxymethyl cellulose/graphene oxide bio-nanocomposite hydrogel beads as anticancer drug carrier agent // Polym., 2017, v. 168, pp. 320–326. https://doi.org/10.1016/j.carbpol.2017.03.014 Worzakowska M. Chemical modifi cation of potato starch by graft copolymerization with citronellyl methacrylate // Polym. Environ., 2018, v. 26, pp. 1613–1624. https://doi.org/10.1007/s10924-017-1062-x Kuznetsov V. A., Kushchev P. O., Blagodat skikh I. V. Aqueous dispersions of cross-linked poly-N-vinylcaprolactam stabilized with hydrophobically modified polyacrylamide: synthesis, colloidal stability, and thermosensitive properties // Polym. Sci., 2016, v. 294, pp. 889–899. https://doi.org/10.1007/s00396-016-3843-5 Gen, Uzun C., Güven O. Quaternized poly(1-vinylimidazole) hydrogel for anion adsorption // Polym. Bull., 2016, v. 73, pp. 179–190. https://doi.org/10.1007/s00289-015-1479-0 Jakubiak-Marcinkowska A, Legan M, Jezierska J. Molecularly imprinted polymeric Cu(II) catalysts with modified active centres mimicking oxidation enzymes // Polym. Res., 2013, v. 20(12), pp. 317–328. https://doi.org/10.1007/ s10965-013-0317-z

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Guptill, David M., Bhavani Shankar Chinta, Trinadh Kaicharla, Shu Xu, and Thomas R. Hoye. "Correction: β-Methyl-δ-valerolactone-containing thermoplastic poly(ester-amide)s: synthesis, mechanical properties, and degradation behavior." Polymer Chemistry 12, no. 21 (2021): 3210. http://dx.doi.org/10.1039/d1py90069b.

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Correction for ‘β-Methyl-δ-valerolactone-containing thermoplastic poly(ester-amide)s: synthesis, mechanical properties, and degradation behavior’ by David M. Guptill et al., Polym. Chem., 2021, 12, 1310–1316, DOI: 10.1039/D1PY00040C.

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Gabirondo, Elena, Ainara Sangroniz, Agustin Etxeberria, Sergio Torres-Giner, and Haritz Sardon. "Correction: Poly(hydroxy acids) derived from the self-condensation of hydroxy acids: from polymerization to end-of-life options." Polymer Chemistry 11, no. 34 (2020): 5548. http://dx.doi.org/10.1039/d0py90135k.

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Correction for ‘Poly(hydroxy acids) derived from the self-condensation of hydroxy acids: from polymerization to end-of-life options’ by Elena Gabirondo et al., Polym. Chem., 2020, 11, 4861–4874, DOI: 10.1039/D0PY00088D.

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Kostić, Marija, Suzana Cakić, Ivan Ristić, Milena Marinović-Cincović, Ljubiša Nikolić, and Suzana Samaržija-Jovanović. "Synthesis and characterization of pH-sensitive saccharide modified Polyurethane hydrogels: Effect of polyol, crosslinker and acid chain extender." Advanced Technologies 10, no. 1 (2021): 29–36. http://dx.doi.org/10.5937/savteh2101029k.

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Biodegradable polyurethanes can be the basis for drug delivery systems that are sensitive to external changes. pH-sensitive polyurethanes (PUs) have been used successfully as intravaginal rings and specific drug delivery systems for the colon. In this study, a series of pH-sensitive polyurethane hydrogels with a change of the polyol component (poly(ethylene glycol) 400/poly(propylene glycol) 2000/poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) 1100), saccharides as crosslinkers (melibiose/raffinose/starch) and a-hydroxy carboxylic acids as chain extenders (dimethylol propionic acid/lactic acid) were synthesized. Structural characterization of the synthesized polyurethane hydrogels was performed using Fourier transform infrared spectroscopy (FTIR), which showed that the polyurethane synthesis reaction was achieved with successful crosslinking with saccharides and, despite the change of starting components, FTIR spectra for all investigated samples are almost identical. The degree of swelling of the hydrogels was monitored at 25 °C in solutions of pH values 4.5 and 7.4. In samples with the polyol component block1100, the degree of swelling at pH 7.4 (16.09%) was up to 9 times higher than at pH 4.5 (1.82%). The effects of variable parameters on the thermal properties and phase transitions of PUs hydrogels were investigated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The results showed that by varying the saccharide as a crosslinker, the acid chain extender and the chain length of the polyols, the stated properties of polyurethane hydrogels as potential drug delivery carriers can be influenced.

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Habich, Malgorzata, Sergej Djuranovic, and Pawel Szczesny. "PATACSDB—the database of polyA translational attenuators in coding sequences." PeerJ Computer Science 2 (February 3, 2016): e45. http://dx.doi.org/10.7717/peerj-cs.45.

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Recent additions to the repertoire of gene expression regulatory mechanisms are polyadenylate (polyA) tracks encoding for poly-lysine runs in protein sequences. Such tracks stall the translation apparatus and induce frameshifting independently of the effects of charged nascent poly-lysine sequence on the ribosome exit channel. As such, they substantially influence the stability of mRNA and the amount of protein produced from a given transcript. Single base changes in these regions are enough to exert a measurable response on both protein and mRNA abundance; this makes each of these sequences a potentially interesting case study for the effects of synonymous mutation, gene dosage balance and natural frameshifting. Here we present PATACSDB, a resource that contain a comprehensive list of polyA tracks from over 250 eukaryotic genomes. Our data is based on the Ensembl genomic database of coding sequences and filtered with algorithm of 12A-1 which selects sequences of polyA tracks with a minimal length of 12 A’s allowing for one mismatched base. The PATACSDB database is accessible at:http://sysbio.ibb.waw.pl/patacsdb. The source code is available athttp://github.com/habich/PATACSDB, and it includes the scripts with which the database can be recreated.

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Sundararajan, G., V. Vasudevan, and K. Audisesha Reddy. "Synthesis of triblock copolymers?(polyA-poly butadiene-polyA)?via metathesis polymerization." Journal of Polymer Science Part A: Polymer Chemistry 36, no. 14 (October 1998): 2601–10. http://dx.doi.org/10.1002/(sici)1099-0518(199810)36:14<2601::aid-pola20>3.0.co;2-l.

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Sorokin, V. A., V. A. Valeev, and E. L. Usenko. "Phase diagram of Ni2+ ions complexes with polyU·polyA·polyU." Biopolymers and Cell 25, no. 3 (May 20, 2009): 218–25. http://dx.doi.org/10.7124/bc.0007df.

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Konopka, Catherine A., Melissa N. Locke, Pamela S. Gallagher, Ngan Pham, Michael P. Hart, Claire J. Walker, Aaron D. Gitler, and Richard G. Gardner. "A yeast model for polyalanine-expansion aggregation and toxicity." Molecular Biology of the Cell 22, no. 12 (June 15, 2011): 1971–84. http://dx.doi.org/10.1091/mbc.e11-01-0037.

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Nine human disorders result from the toxic accumulation and aggregation of proteins with expansions in their endogenous polyalanine (polyA) tracts. Given the prevalence of polyA tracts in eukaryotic proteomes, we wanted to understand the generality of polyA-expansion cytotoxicity by using yeast as a model organism. In our initial case, we expanded the polyA tract within the native yeast poly(Adenine)-binding protein Pab1 from 8A to 13A, 15A, 17A, and 20A. These expansions resulted in increasing formation of Pab1 inclusions, insolubility, and cytotoxicity that correlated with the length of the polyA expansion. Pab1 binds mRNA as part of its normal function, and disrupting RNA binding or altering cytoplasmic mRNA levels suppressed the cytotoxicity of 17A-expanded Pab1, indicating a requisite role for mRNA in Pab1 polyA-expansion toxicity. Surprisingly, neither manipulation suppressed the cytotoxicity of 20A-expanded Pab1. Thus longer expansions may have a different mechanism for toxicity. We think that this difference underscores the potential need to examine the cytotoxic mechanisms of both long and short expansions in models of expansion disorders.

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Tinajero-Perez, Enrique J., Jesus Ezequiel Molinar-Solis, Rodolfo Z. Garcia-Lozano, Pedro Rosales-Quintero, Jose M. Rocha-Perez, Alejandro Diaz-Sanchez, and Arturo Morales-Acevedo. "Fowler-Nordheim Tunneling Characterization on Poly1-Poly2 Capacitors for the Implementation of Analog Memories in CMOS 0.5 μmTechnology." Advances in Condensed Matter Physics 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/632785.

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The experimental results of the Fowler-Nordheim characterization using poly1-poly2 capacitors on CMOS ON Semi 0.5 μm technology are presented. This characterization allows the development, design, and characterization of a new current-mode analog nonvolatile memory. Experimental results of the memory cell architecture are presented and demonstrate the usefulness of the proposed architecture.

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Arthur, Laura L., and Sergej Djuranovic. "PolyA tracks, polybasic peptides, poly-translational hurdles." Wiley Interdisciplinary Reviews: RNA 9, no. 5 (June 5, 2018): e1486. http://dx.doi.org/10.1002/wrna.1486.

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Su, Ye, and Wang Wan Jiang. "Thermal stability of poly(oxypropylene-ether) polyol." Thermochimica Acta 123 (January 1988): 221–31. http://dx.doi.org/10.1016/0040-6031(88)80026-1.

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Aoyanagi, Ousuke, Nobuhiro Muramatsu, Hiroyuki Ohshima, and Tamotsu Kondo. "Electrophoretic Behavior of PolyA-graft-PolyB-Type Microcapsules." Journal of Colloid and Interface Science 162, no. 1 (January 1994): 222–26. http://dx.doi.org/10.1006/jcis.1994.1029.

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Sorokin, V. A., V. A. Valeev, and E. L. Usenko. "Ni2+ ion effect on conformational equilibrium of polynucleotides: polyA·polyU, polyA and polyU under conditions close to physiological ones." Biopolymers and Cell 24, no. 2 (March 20, 2008): 158–70. http://dx.doi.org/10.7124/bc.00079d.

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Lee, Sang-Hyub, Cho-Rong Oh, and Dai-Soo Lee. "Large Improvement in the Mechanical Properties of Polyurethane Nanocomposites Based on a Highly Concentrated Graphite Nanoplate/Polyol Masterbatch." Nanomaterials 9, no. 3 (March 7, 2019): 389. http://dx.doi.org/10.3390/nano9030389.

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In this study, a highly concentrated graphite nanoplate (GNP)/polyol masterbatch was prepared by the exfoliation of natural graphite in an aqueous system using cetyltrimethylammonium bromide and the replacement of aqueous solution with a polyol, viz. poly(tetramethylene ether glycol), and it was subsequently used to prepare polyurethane (PU) nanocomposites by simple dilution. The polyol in the masterbatch efficiently prevented the aggregation of GNPs during the preparation of PU nanocomposite. In addition, the dispersed GNPs in the masterbatch exhibited rheological behavior of lyotropic liquid crystalline materials. In this study, the manufacture and application methods of the GNP/polyol masterbatch were discussed, enabling the facile manufacture of the PU/GNP nanocomposites with excellent mechanical properties. In addition, the manner in which the GNP alignment affected the microphase separation of PU in the nanocomposites was investigated, which determined the improvement in the mechanical properties of the nanocomposites. High-performance PU/GNP nanocomposites are thought to be manufactured from the GNP/polyol masterbatch by the simple dilution to 0.1 wt% GNP in the nanocomposite.

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Journal articles on the topic 'Polyl'

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