Dissertations / Theses: 'Polymorfismus'

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Ječmínková, Kateřina. "Vztah genů pro imunitní systém k funkčním vlastnostem (reprodukce a zdraví) u skotu." Doctoral thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-259726.

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In cattle, we observe a decrease trend in the level of reproduction and animal health. This decline is associated with an increase in milk yield. This situation has a negative impact on the farm economy, animal welfare and safety of product. The traditional selection program is in these so-called functional traits ineffective and there is need to find a new ways of animal breeding. In this problematic traits marker assisted selection and the related genomic animal selection is proposed to be a good tool for animal breeding. The present work summarizes the problems of selected functional traits in cattle and provides an overview on genetic markers and possibilities for their use. Further attention is paid to the studied genes and their associations to functional features which have been published in the scientific literature. At the end, the first results are presented describing the genotype frequencies for the first four studied polymorphic regions in Czech Fleckvieh cattle.

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Šalanda, Ondřej. "Strojové učení v úloze predikce vlivu nukleotidového polymorfismu." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234918.

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This thesis brings a new approach to the prediction of the effect of nucleotide polymorphism on human genome. The main goal is to create a new meta-classifier, which combines predictions of several already implemented software classifiers. The novelty of developed tool lies in using machine learning methods to find consensus over those tools, that would enhance accuracy and versatility of prediction. Final experiments show, that compared to the best integrated tool, the meta-classifier increases the area under ROC curve by 3,4 in average and normalized accuracy is improved by up to 7\,\%. The new classifying service is available at http://ll06.sci.muni.cz:6232/snpeffect/.

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Kaňková, Veronika. "Stanovení různých forem kaseinu v mléce." Master's thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-259391.

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Casein proteins are important escpecially for cheese making. In addition to technology and other factors, casein concentration in milk and relative representation of individual casein fractions influence cheese yield. Majority fractions are alfa-casein, beta-casein and kappa-casein. For the determination of proteins in the milk can be used electrophoretic techniques or near infra-red spektrometry. High performance liquid chromatography (HPLC) is going to be used. HPLC is widely used analytical method, which is based on differential affinity of the substances to the mobile and the stationary phase. The thesis has introduced the method of determining the casein fractions by HPLC. The method was partially optimized using cow milk and calibrated to the standard solutions, which were purchased casein fractions derived from the bovine milk. Real samples of cow, sheep and goat milk were analyzed. The goat milk was available in several variants: Sanski goat milk and white shorthair goat from domestic or farm breeding. All milk samples were analyzed for casein and casein fractions proportions. Chromatograms show that the casein formula is different in cow, sheep and goat milk. Different concentrations of the casein fraction in the milk of various animal species have been statistically detected in almost all cases. The exception was the concentration of beta-casein in the milk of white shorthair goat from the domestic and the farm breeding and cow milk. In these types of milk there was no statistically significant difference in the concentration of beta-casein. Analysis of individual samples of white shorthair goat milk from the farm breeding shown that casein content in the milk of different animals reared under the same conditions may be different. Ratios of beta-casein to alfa-casein, beta-casein to kappa-casein and alfa-casein to kappa-casein were evaluated in all samples. Interspecific comparisons proved that ratios were statistically significantly different in all milks. In the milk of Sanski goat, there was found the stable ratio of beta-casein to alfa-casein during lactation. For all goat milk there were typical higher values of the ratio of beta-casein to kappa-casein. The ratio of alfa-casein to kappa-casein was highest in the sheep milk. Despite some shortcomings, it is possible to use the HPLC method possible for these purposes, but it is necessary to continue with its optimization.

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Novák, David. "Studium polymorfie a optimalizace krystalizace farmaceuticky aktivních látek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216442.

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Active pharmaceutical ingredients (APIs) are frequently delivered to the patient in the solid-state as part of an approved dosage form (tablets, capsules, etc.). Understanding and controlling the solid-state chemistry of APIs is therefore an important aspect of the drug development process. APIs can exist in a variety of distinct solid forms, including polymorphs, solvates, hydrates, co-crystals and amorphous solids. Each form displays unique physicochemical properties that can profoundly influence the bioavailability, manufacturability, stability and other performance characteristics of the drug. Most APIs are purified and isolated by crystallisation from an appropriate solvent during the final step in synthetic process. The main objective of a crystallisation process is to produce crystals with desired properties such as particle size distribution (PSD), shape and purity. All pharmaceutical dosage forms must be produced in uniform units, and good content of uniformity is only possible when the size of the active component is carefully controlled. For on-line control of crystallisations of Quetiapine Fumarate to achieve desired PSD and no changed physicochemical purity was used the Lasentec Focus Beam Reflectance Measurement (FBRM) system.

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Crapanzano, Laura. "Polymorfisme du soufre : une étude structurale et dynamique." Université Joseph Fourier (Grenoble), 2006. http://www.theses.fr/2006GRE10054.

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Cette thèse est une contribution à l’étude du polymorphisme du soufre à l’état solide et à l’état liquide avec, en particulier un examen des transitions liquide-liquide. Une introduction sur le polymorphisme dans les matériaux simples conduit à faire le choix d’étudier le soufre et on présente l’état des connaissances actuelles sur le diagramme P T du soufre. Puis on décrit les dispositifs haute pression; enclume diamant et presse à grand volume, mis en place à l’ESRF pour réaliser des études structurales ou dynamiques in situ, ainsi que les techniques d’étude de la structure et de la dynamique ; diffraction X, diffusion Raman, diffusion X inélastique utilisés pour ce travail. La partie suivante expose les résultats obtenus sur la structure des différents allotropes du soufre. Grâce à la possibilité de mesures in situ avec un flux intense et à l’utilisation conjointe de la diffraction X et de la diffusion Raman il a été possible de caractériser la structure de phases à l’équilibre et de construire d’un nouveau diagramme de phases du soufre en pression et température. La dernière partie concerne les résultats obtenus sur la dynamique du soufre liquide autour de la transition de polymérisation. On caractérise ainsi, aux basses températures, un liquide moléculaire classique dont la dynamique est celle des molécules S8. Aux plus hautes températures une solution de polymères présente encore le mode à haute fréquence correspondant aux molécules S8 mais aussi un autre, à basse fréquence, qui correspond à des fragments de chaînes interconnectes
This thesis deals with an investigation of polymorphism in both solid and liquid state of sulphur. Emphasis has been put on the polymer transition of liquid sulphur. From a short introduction to polymorphism, sulphur appears as a convenient system for these studies. The state of the art of the P T diagram of sulphur is discussed. Then the manuscript describes the high pressures devices and introduces the ESRF used techniques in order to get either structural or dynamical in situ information; namely X ray scattering, Raman scattering and inelastic X ray scattering. The following part is devoted to the structural results obtained on several sulphur allotropes. Owing to the possibility of in situ measurements with high flux and by using together X-ray and Raman scattering, the structure of equilibrium phases has been determined. Then a new T P phase diagram of sulphur has been obtained. The last part concerns the dynamical results obtained in the temperature range of the polymerisation transition. Above the transition sulphur behaves as a conventional molecular liquid made of S8 units. At higher temperature it still exhibits S8 molecular motion. However a lower frequency mode shows up. It corresponds to the dynamics of cross linked chain fragments

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Smajlovic, Dzenan. "Bestämning av FTO (Fat mass and obesity associated gene) polymorfism." Thesis, University of Kalmar, School of Pure and Applied Natural Sciences, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-804.

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Vetenskapen har på senare år försökt fastställa de olika orsaker som leder till fetma. Det är känt att högt energiintag och för lite motion för eller senare hos de flesta individer resulterar i fetma. Det som kan konstateras är att ärftlighet i samspel med miljön vi lever i och påverkas av kan vara den huvudsakliga orsaken till en rad sjukdomar inklusive fetma. På senare år har forskare upptäckt olika gener som på ett eller annan sett är involverade i ämnesomsättningen. En sådan gen är ”fat mass and obesity associated gene”, FTO. Denna gen återfinns på kromosom 16 och har en storlek på 410 kilobaspar. Genen består av nio kodande områden, exoner, och 8 icke kodande områden, introner. Genens funktion är inte fastställd men den tycks både reglera ämnesomsättningen och lipolysen i kroppen. Tidigare studier har konstaterat att en specifik polymorfi i nukleotid rs9939609 medför ökad risk för sjuklig fetma. Uppsättningen som förekommer i nukleotiden uttrycks med A och T. Där dubbel uppsättning av A- allelen klassas som ärftlig risk för fetma. Syftet med detta examensprojekt är att bestämma polymorfi hos FTO genen med hjälp av två olika pyrosekvenserings- baserade metoder. Metod 1 bygger på extraktion av DNA från helblod, sedan amplifiering med PCR och slutligen pyrosekvensering. Metod 2, som jämfördes med metod 1, bygger på PCR direkt på helblod och pyrosekvensering. Blod från 97 friska individer analyserades. Med metod 1 konstaterades förekomst av följande genotyper i provmaterialet, 11 A/A homozygota, det vill säga har riskallelen för fetma i dubbeluppsättning, 50 A/T heterozygota och 36 T/T, vildtyp, som står för minskad ärftlig risk för fetma respektive ingen alls. Med metod 2 som skulle testas, visade sig resultatet överensstämma med metod 1. Med metod 2 erhölls följande resultat 11 A/A, 49 A/T och 34 T/T. Med metod 2 kunde inte 3 prov analyseras. Slutsatsen som kan dras utifrån studien i detta projekt är att metod 2 är likvärdig metod 1 ur analyssynpunkt. Metod 2 är arbetsbesparande tidsmässigt och även billigare då DNA extraktionssteget inte behöver genomföras.

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Science has for a long time looked for an answer for obesity. Obesity is often explained as the problem of the energy we eat and don’t use, but obesity might also have hereditary causes, where specific genes might play an important role. One of the recent genes found is the fat mass and obesity associated gene, FTO, which is located on chormosome 16 and has a size of 410 kilobasepairs. The gene is composed of nine exons and eight introns. The function of the gene is not known in detail, but studies has indicated that the gene could play a part in regulating the metabolism and fat cell lipolysis. The purpose with this examination degree project was to compare two methods for analysis of polymorphism in the FTO gene. Method 1 is based on DNA purification from whole blood, amplification with PCR, and finally detection using pyrosequencing. In method 2 PCR is performed on whole blood directly without prior DNA purification. Pyrosequencing was used with this method also to detect the polymorphism. Earlier studies have shown that theSNP (single nucleotid polymorphism) rs9939609, is associated with increased risk for obesity. Results obtained using method 1 were, 11 individuals had the A/A genotype, 50 was heterozygous (A/T), and 36 the wild type form (T/T), that is not associated with an increased risk for obesity. With method 2, the same result as with method 1 was obtained for the 94 samples of blood analyzed; 11 A/A, 49 A/T and 34 T/T were obtained. Remaining three samples of the 97 analyzed, failed in the pyrosequencing with method 2.

The conclusions with this degreeprojcet were that method 1 and 2 gave the same results. Method 2 is recommended as it is faster and less expensive, as no prior DNA purification is needed.

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Novák, Samuel Matyáš. "Implementace součtového datového typu v C++." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2020. http://www.nusl.cz/ntk/nusl-416668.

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Práce se zaobírá problematikou implementace součtového datového typu v jazyce C++. Nejprve navrhuje vlastní abstrakce pro manipulaci s posloupnostmi hodnot a typů, které následně umožňují vyjadřovat komplexní operace deklarativně. V průběhu implementace jsou prezentovány návrhové vzory obvyklé pro oblast metaprogramování. Implementace přichází s rozhodnutími odlišnými od referenční implementace. Pro manipulaci se součtovým typem jsou představeny dvě šablony funkce, lišící se sémantikou. Užití součtového typu je ukázano na příkladu konečného stavového automatu.

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Trýznová, Alena. "Polymorfismus v genu MDR1 u border kolií." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-430483.

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This thesis is focused on polymorphism of MDR1 gene c.-6-180T>G, which causes phenobarbital resistance. Phenobarbital is one of the most commonly used drug against epilepsy, which is one of the most common neurological disease in dogs with frequency between 0,5-5 % according to breed. This mutation has so far been detected only in the border collie breed, which manifests up to 30 percent resistance to the treatment of epilepsy. In the text is subscribed border collie breed, dog epilepsy and gene MDR1. Methodical part is focused on detection of the polymorphism in model population of 82 dogs. The results agree with previous studies and confirm frequency of mutant alleles 32 % within the population.

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KRÁLOVÁ, Martina. "Polymorfismus mikrosatelitových markerů u kmenů \kur{Beauveria bassiana}." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-79703.

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\kur{Beauveria bassiana} is a entomopathogenic polyphagous fungus commonly found in soil and it is parasite of soil insects, mainly of the stages of insect that occur in soil. At the present time it is used in plant protection against more than 70 species of insects. In the Czech Republic \kur{Beauveria bassiana} has the greatest importance in the fight against bark beetle \kur{Ips typographus} in the NP Šumava in these days. This study was focused on the evaluation of genetic variability \kur{Beauveria bassiana} strains on the basis of microsatellite analysis and the comparison of four separation methods: electrophoresis in 2% agarose gel, electrophoresis in 3% synergel, chip electrophoresis and fluorescent capillary electrophoresis in term of the most precise separation of PCR products. We used 41 strains which were collected in the NP Šumava and 20 strains from long-term collection determined as an exotic in this study. This large geographical scale group contains the strains from whole world and in addition it was upgraded by the strains collected from the NP Krkonoše and South Moravia. For the microsatellite analysis there were used 11 pairs of primers but for inter-comparison of separative methods were chosen only 4 pairs of primers. The population of \kur{Beauveria bassiana} strains collected from the NP Šumava were evaluated by analysis of microsatellites as a conservative and fully closed regardless of the source and the location. The strains from the large geographical scale group showed the great genetic variability. In terms of separation, the best and most suitable separation method was proved, the fluorescent capillary electrophoresis. Despite of its difficult financial aspect, this method was evaluated as the most precise and the most sensitive. Its advantage is in possibility to detect the smallest differences in the length of single allele in the range 1-2 bp, which is for the gel electrophoresis impossible.

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Červinková, Barbora. "Genetický polymorfismus v HFE genu v české populaci." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-309360.

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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Barbora Červinková Supervisor: Doc. PharmDr. Martin Beránek, Ph.D. Title of diploma work: Genetic polymorphism of the HFE gene in Czech population Outline: The main objective of the thesis was to map the incidence of the three mutations (C282Y, H63D, S65C) in the HFE gene in the Czech population. Consequently, the obtained results were compared with the reported occurrence in the world. Methods: The work involved the isolation of DNA from buccal swabs obtained from 167 donors (65 men, 102 women; average age for men was 31, for women 28). Isolated DNA strings were amplified by PCR methods using 5'-CAG ATC CTC ATC TCA CTG-3' and 5'-CTG GAT AAC CTT GGC TGT ACC CCC-3' primers for C282Y mutation, 5'-GCC ACA TCT GGC TTG AAA TT-3' and 5'-ACA TGG TTA AGG CCT GTT GC GCC ACA- 3' primers for H63D and S65C mutations. DNA samples were treated with restriction enzymes Rsa I, Bcl I and Hinf I, for C282Y, H63D and S65C mutations, respectively. Finally, the restriction fragments were separated by gel electrophoresis (2 % agarose gel). Results: C282Y mutation was present in 0 (0 %) and 19 (11,38 %) samples as homozygote or heterozygote, respectively. Moreover, C282Y mutation was present in 3 (1,80 %)...

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Pšikalová, Renata. "Polymorfismus Q192R lidské paraoxonasy (PON1) u hemodialysovaných pacientů." Master's thesis, 2006. http://www.nusl.cz/ntk/nusl-272181.

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Kopečný, Michal. "Polymorfismus vybraných kandidátních genů pro znaky jatečné hodnoty prasat." Doctoral thesis, 2000. http://www.nusl.cz/ntk/nusl-95690.

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Horecká, Eliška. "Polymorfismus genu MATP ve vztahu ke zbarvení u koní." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-169462.

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Těšický, Martin. "Mezidruhový polymorfismus vybraných genů vrozené imunity u sýkor (Paridae)." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-411457.

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Adaptation of host receptor system to optimal detection of infection-related structures is one of the key evolutionary challenges of immunity in host-pathogen interactions. Toll-like receptors (TLRs) are genetically variable molecules of vertebrate innate immunity that recognise danger signals, e.g. pathogenic molecules. Examination of genetic variation in TLRs may reveal mechanisms of host immunity adaptation to pathogenic pressure at molecular level. Trans-species polymorphism (TSP) is a phenomenon which assumes that several identical alleles or allelic lineages are inherited from ascendant to descendant species and these may be subsequently maintained over a long period of time in a polymorphic state. Whereas in adaptive immune genes the concept of TSP is well understood, little is presently known about TSP in innate immune genes such as TLRs. In this thesis I describe genetic polymorphism in functionally-relevant regions of TLR4 and TLR5 in 192 individuals representing 20 species Paridae family (tits, chickadees and titmice). These two receptors bind mainly bacterial ligands (TLR4 detects lipopolysaccharide and TLR5 detects flagellin), being among the first ones to trigger immune response to bacterial pathogens. To differentiate presumed TSP from gene flow among species, intron sequences of six...

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KLOJDA, Martin. "Polymorfismus vybraných enzymů jako faktor ovlivňující složení mléčného tuku." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-395066.

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The aim of this diploma thesis was to determine allele and genotype frequencies for g.10329C>T (A293V) polymorphism in the SCD1 gene in dairy cow populations, establish effects of this locus on milk production traits and fatty acids composition. At first, allele and genotype frequencies were determined. Subsequently the effect of this polymorphism on milk production traits was proved and effects of this polymorphism were demonstrated as well. For the determination allele and genotype frequencies the PCR/RFLP technique was applied. In this diploma thesis the effect of polymorphism g.10329C>T (A293V) on milk production traits was demonstrated as well as the effects of this polymorphism on fatty acids composition. The findings following from this diploma thesis show economical and health benefits of this SCD1 locus.

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Bučková, Jana. "SNP polymorfismus na Y chromozomu u populace afrických Fulbů." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-285213.

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Markers on the non-recombining region of chromosome Y is a useful tool for study of diversity between populations. SNPs are the most commom polymorphisms in human genome. Mutation rate of SNPs is very low and so they may be used as genetic markers in evolutionary and population studies. We have analyzed 205 unrelated men from 11 Sub-Saharan Fulani's subpopulations. Fulani are an ethnic group of people spread over many countries, mainly in West Africa. Our samples are from Tindangou area, Banfora area (Burkina Faso), Bongor area, Linia area (Chad), Diafarabé area (Mali), Tcheboua area (Cameroon), Banfora area, Diffa area, Zinder area, Ader area and Abalak area (Niger). Using kit Signet Y-SNP Identification Systems and Luminex instrument with LabMAP Luminex Technology we detected particular Y chromosome's SNPs. LabMAP Luminex Technology is universal array platform, which as a probe using fluorescent polystyrene microspheres. We have observed 12 different haplogroups. Haplogroup E, which is typical African haplogroups, is determined with derivated allele in polymorfism M96. We have detected haplogroup E in maximum of 89,3% in the Fulani's subpopulations. In 7,8% we have detected haplogroup R, which is characteristic of populations in the Euroasia. Gene pool of Fulani's population is influenced with a...

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Sosna, Ondřej. "Genový polymorfismus Th1/Th2 cytokinů u pacientek s děložní myomatózou." Doctoral thesis, 2011. http://www.nusl.cz/ntk/nusl-311446.

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Background: Uterine fibriod (UF) or leiomyoma is the most frequent benign tumour upon lower genital tract and represents the most frequent indication for hysterectomy. The aetiology remains still unknown. The genetic factors contributing for the development of UF are being intensively investigated. The aim of our study was to look for possible genetic markers which could be used as prognostic tools for evaluation of an increased risk for development of UF. Methods: The study group enrolled 102 patients diagnosed with UF and 145 healthy controls. Ultrasonographic examination of the pelvis was performed and a single blood sample was taken in all women. Histological verification followed the surgery in the patient group. The principal of the cytokine gene polymorphisms detection is based on PCR reaction with sequence-specific primers. Results: A large spectrum of Th1/Th2 cytokine gene polymorphisms in patients with uterine fibroid was compared with control group. The frequencies of the majority of tested cytokine gene SNP in the patient cohort were not statistically different from the cytokine SNP in the control group. However, an intriguing association between polymorphisms of the IL-4 gene promotor at positions -590 C/T and -33 C/T, and the risk of leiomyoma was observed. The CC genotype of IL-4 at position...

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TYLLEROVÁ, Helena. "POLYMORFISMUS GENU PRO SERICIN 2 U BOURCE MORUŠOVÉHO (BOMBYX MORI)." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-53358.

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In our study, we examined the polymorphism of gene Ser2 from domesticated silkmoth Bombyx mori and its closest wild relative B. mandarina. As a starting material for our work, we used the restriction map of allele C isolated from hybrid lineages 200 and 300 of European silkmoth B. mori (Michaille et al. 1990a). We also used the published sequence of allele D which was isolated from {\clq}qDaizo`` p50 strain of B. mori (Kludkiewicz et al 2009). Based on the published sequence, we designed PCR primers and performed sequence analysis of 4 different alleles of gene Ser2, including the almost complete genomic sequence of allele C (except for two short regions containing repetitive DNA). We then compared the sequences and found that the alleles differ significantly not only in intron lengths and the sizes of exon 9, but also in exon arrangements. While the allele C contains 12 exons (1, 2, 3, 4, 5, 6, 7, 8a, 9, 10, 11, 8b), the alelle D and other alleles analysed have 13 exons (1, 2, 3, 4, 5, 6, 7, 8, 9a, 10a, 9b, 10b, 11). Allele C showed very unique arrangement of exons, which suggested that it may represent an ancestral form of Ser2 gene. We analysed the arrangment of exons located at the 3´end of the Ser2 gene by PCR and electrophoresis in 70 available strains of B. mori and B. mandarina. We also tried to find a similar allele to the allele C and we amplified and sequenced 2 kb region from 55 Bombyx strains. Phyllogenetic analysis of gene Ser2 gene suggested that the C allele of now probably extinct European silkmoth hybrids 200 and 300 does not seem to be an ancestral form of Ser2 gene, but it rather seems to be the result of complex secondary rearrangements.

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Kučírek, Martin. "Polymorfismus v genu DGAT1 ve vztahu k marblingu u skotu." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-179347.

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Intramuscular fat occurring in the muscle of cattle is supposed to be prerequisite for high-quality beef. Marbling has effect on juiciness, tenderness and flavor of the meat. Selection of cattle at higher marbling score brings increased meat tenderness. It is influenced by many factors, among which we classify genetic background, age of animal, nutrition, sex and pedigree of animal. The aim is to perform association analysis beetween dinucleotide polymorphism in the gene DGAT1 (diacylglycerol acyltransferase 1) and the marbling score. Part of thesis is elaboration of literature, present state of the problem, genes affecting metabolism of intramuscular fat in cattle, the role of the enzyme DGAT1 in the biosynthesis of triacylglycerols and its physiological function (energy metabolism, glucose metabolism, relation to fat accumulation and obesity). In this study a singificant difference of allelic contribution to intermuscular fat content in tested samples was found. Nevertheless, effect of Q allele was reverse than observed in similar studies.

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ŠVARCOVÁ, Monika. "Polymorfismus kandidátního lokusu a jeho vliv na technologickou jakost mléka." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-375813.

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This thesis was developed to study the influence of polymorphic variants of the milk protein locus betacasein (BCN) on the production and technological quality of the milk. The genotypes of -casein (CSN2 gene) were determined by the PCR-RFLP analysis. The determination of genotypes AA, AB and BB was made in 731 dairy cows. The results of genotype frequencies showed that genotype BB represented roughly five times more frequently than the heterozygotes AB and thirty times more frequently than AA homozygotes in the population under study. The effect of the BCN genotypes on the production and composition of milk was analyzed in 418 samples. No statistically significant effect of genotypes or alleles was found in this section. Subsequent analysis of the influence of genotypes and allele on the coagulation and the determination of titratable ability of the milk was performed on 119 samples. The results did not confirm a statistically significant effect on milk coagulation. The yogurt test showed genotypic effects. This could mean that genotype AA is associated with higher milk acidity and AB genotype with lower. However, the results could be affected by lower frequency of the population and an uneven frequency of genotypes in the population surveyed.

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Sekal, Ladislav. "Testování mikrosatelitu v genu pro visfatin a asociace k užitkovým vlastnostem u přeštického černostrakatého prasete." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-88019.

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Heczková, Jaroslava. "Analýza primární struktury genu MC3R pomocí metody resekvenování DNA." Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-95069.

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Weisz, Filip. "Polymorfizmy DNA v genu PRNP u vybraného souboru skotu." Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-95494.

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Stefek, Tomáš. "Studium polymorfismu genu LDHA a jeho vliv na masnou užitkovost u prasat." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-169853.

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Vašáková, Martina. "Th1/Th2 genový polymorfismus cytokinů u pacientů s idiopatickou plicní fibrózou." Doctoral thesis, 2007. http://www.nusl.cz/ntk/nusl-288909.

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Objectives: Idiopathic pulmonary fibrosis (IPF) is a serious disease characterized with progressive scarring of the lungs in which the genetic background is supposed. The aim of our study was to investigate Th1/Th2 cytokine gene polymorphisms to evaluate their possible influence on IPF development. Then we have correlated selected polymorphisms of IL-1, IL-4 and IL-12 groups ( the selection was based on our previous results) with clinical parameters and high resolution computed tomography (HRCT) as a markers of disease stage and progression. Methods: We investigated 30 patients with IPF and 103 healthy volunteers for the cytokines polymorphisms of the IL-1 alpha, IL-1 beta, IL-1R, IL-1RA, IL-2, IL-4, IL-6, IL-10, IL-12, TNF alpha, IFN gamma, TGF beta, IL-1 beta, IL-2, IL-4 and IL-4RA genes. The PCR-SSP method was used for measurement. Then the correlations of vital capacity(VC) and diffusing capacity for carbon monoxide(DLCO), bronchoalveolar lavage (BAL) fluid cell counts and high resolution computed tomography (HRCT) alveolar and interstitial scores with different genotypes of groups of IL-1, IL-4, and IL-12 cytokines and their receptor antagonists. The HRCT results were evaluated by an experienced viewer using the interstitial and alveolar score scales, which were based on the IPF HRCT description system...

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VERNEROVÁ, Kateřina. "Genetický polymorfismus vybraných kódujících lokusů ve vztahu k technologickým vlastnostem masa." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-137050.

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Within the framework of this diploma, a genotypization of 162 beef cattle of the ČESTR commercial breed from was performed in order to analyze the potential influence of gene coding calpain I (CAPNI) on the resulting beef tenderness. CAPN1 present on the BTA29 chromosome, was selected in previous studies as a candidate gen for QTL. Polymorphism of CAPNI gene was studied using PCR/RFLP method and CAPN530 marker. Genotype identification resulted from this procedure. A 341 bp long fragment was present in homozygotes AA, two fragments of 195 and 146 bp were present in homozygotes GG, and three fragments of 341, 195 and 146 bp were detected in heterozygotes AG. The genotypization output was subsequently statistically evaluated. 11 homozygotes AA, 62 homozygotes GG and 89 heterozygotes AG were detected in the analyzed beef cattle population. More frequent G allele occurred in the set with the frequency of 0,657 and A allele with the frequency of 0,343. Warner-Bratzler shear test was employed to determine beef tenderness based on the shear force attribute. Samples of raw and grilled beef aging 1, 14 and 28 days after the slaughter were analyzed. Statistical methods were used to evaluate relationship between genotype and detected amount of shear force. In case of raw beef, only the samples aging 1 day after the slaughter showed significant difference in the shear force. The most positive results for the given parameter were achieved in case of AG genotype. Samples aging 14 and 28 days after the slaughter showed no difference in shear force and related beef tenderness, indicating no genotype influence. In case of grilled beef, no significant difference in sheer force indicating possible genotype influence was detected at any day of analysis. Genotype influence on grilled beef tenderness within the test animal population is minimal and statistically inconclusive.

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Svobodová, Lucie. "Polymorfismus genu EDNRB ve vztahu ke zbarvení a onemocnění u koní." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-169870.

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Hosnedlová, Božena. "Metodika a asociace polymorfismu IGF1 u drůbeže." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-87586.

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Kadlecová, Jana. "Identifikace odrůd pšenice obecné prolaminovými bílkovinami obilky." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-90098.

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Moravanská, Andrea. "Vztah polymorfismu UASMS1 v genu leptin s ukazateli kvality masa." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-362855.

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The aim of this thesis was to perform association analysis of UASMS1 polymor-phism in leptin gene with parameters of beef quality, studied on a group of Czech Fleckvieh bulls. The group of 236 bulls was genotyped by direct sequencing of the PCR product. Individual genotypes were processed into a database and statistical evaluation of association was performed by using SAS software. The UASMS1 polymorphism had a highly significant effect on palmitoleic acid content (p = 0.00006) and stearic acid content (p = 0.0005) between CT and TT genotypes. There was a significant value in myristoleic acid content (p = 0.0117) between genotypes CT and TT and in linolelaidic acid content between both genotypes CC and CT (p = 0.0340) and CC and TT (p = 0.0417). Values relating to the effect on dry matter content (p = 0.0746) and colour of meat (p = 0.0541) had only approaching evidentiary values. The influence of genotype on intramuscular fat content or other parameters of beef quality has not been demon-strated in this study.

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ČERMÁKOVÁ, Táňa. "Vliv vybraných abiotických faktorů na křídelní polymorfismus hladinatky pobřežní (Microvelia reticulata Burmeister, 1835)." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-47726.

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Wing polymorphism of semiaquatic bugs is affected by environmental factors as temperature, photoperiod, population density or permanency of habitat. The heritability has its own specific part, too. A lot of field studies suggest that crucial effect on wing polymorphism has temperature, but laboratory experiments often do not confirm this hypothesis. The main aim of this thesis was to describe the effect of environmental factors (photoperiod, temperature and permanency of habitat) on wing polymorphism of Microvelia reticulata, additionally of Gerris lacustris. The partial tasks included description of effect of mentioned abiotic factors on mortality and rate of development. The individuals of both species were kept at combinations of two levels of temperature, photoperiod and permanency of habitat (overall at eight treatments) from first larval instar to adult. All adults of Microvelia reticulata emerged as apterous, so there was no response of wing polymorphism to tested environmental factors. The main influence of heritability is presumable. Only ten individuals of G. lacustris developed into the adult stage, so it was impossible to describe the influence of abiotic factors on wing polymorphism, mortality and lenght of development in this species. Mortality of individuals of M. reticulata was significantly influenced by the type of substrate. Only 3.5% of individuals survived when reared on wet filter paper, while 80.5% on the water surface. Both other factors (temperature, photoperiod) had no effect on total mortality. There was no difference between mortality of males and females, so it is impossible to note sexual dependent mortality. The lenght of development did not differ between sexes either. Lenght of development was significantly affected by temperature, as low temperature reduces development of M. reticulata. The fastest development showed nymphs of second instar and slowest development nymphs of fifth instar at all treatments. Since the effect of abiotic factors on wing polymorphism was not found in M. reticulata, it would be sufficient to study the effect of heritability on development of wings in this species in the future.

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Rataj, Michal. "Genetický polymorfismus v NBS1 genu pro diagnostiku a léčbu osob s cervikálním karcinomem." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-324653.

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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Michal Rataj Supervisor: Doc. PharmDr. Martin Beránek Ph.D. Title of diploma thesis: Genetic polymorphism in the NBS1 gene for diagnosis and treatment of patients with cervical carcinoma The aim of this diploma thesis is to find optimal methods for screening of mutation 657del5 and estimate frequency of heterozygotes and homozygotes for the mutation 657del5 in population of the Czech republic. In the first section of the theoretical part is comprehensively pointed out the effect of factors affecting the integrity of genetic information and the formation of mutations in DNA. On the contrary, the second section devotes to the ability of cells to respond to this damage. In detail, the thesis devotes to the NBS1 gene and its product nibrin. In the complex MRE11/Rad50/NBN nibrin is an important member of the mechanisms of repair of double strand breaks NHEJ (non-homologous end joining) and HR (homologous recombination). The thesis is focused on nibrin and its functions, but also to mutations that prevent these functions and causes genetic disease Nijmegen breakage syndrome. Nibrin is translated from the sequence of the NBS1 gene. Gene NBS1 appears in population with several various...

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Křenková, Leona. "Vztah polymorfismu kandidátních genů k proměnlivosti produkce a kvality masa prasat." Doctoral thesis, 1999. http://www.nusl.cz/ntk/nusl-95324.

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KREMLOVÁ, Markéta. "Vliv abiotických a biotických faktorů na polymorfismus barvy květů u \kur{Dactylorhiza sambucina} (Orchidaceae)." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-50747.

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The European rewardless orchid Dactylorhiza sambucina commonly produces yellow- and purple-flowered individuals in frequencies that in different populations range from balanced to very unbalanced ones (we can find even monochromatic populations). I studied an effect of abiotic factors (soil pH, hydrolimits, slope and altitude of the locality), biotic factors (weight and viability of the seeds obtained from crossing between and within morphs) and the size of the populations on corolla colour of two morphs in European populations (the Czech Republic, Italy, Austria, Germany, France).

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Nguyen, Chi Mai. "Polymorfismus genu kódujícího enzym GBSS I u vybraných planých a kulturních druhů pšenic (Triticeae)." Master's thesis, 2006. http://www.nusl.cz/ntk/nusl-373625.

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Mazalová, Lenka. "Variabilita genu LEPR a jeho asociace s ukazateli produkce vepřového masa." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-169658.

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Knoll, Aleš. "Detekce polymorfismu DNA ve vztahu k mapování QTL u prasat." Doctoral thesis, 1998. http://www.nusl.cz/ntk/nusl-94952.

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Hovhannisyan, Milena. "Polymorfismus rs1801282 v genu PPARγ ve vztahu ke koncentraci a složení mastných kyselin u českých adolescentů." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-436137.

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The prevalence of obesity and type 2 diabetes in the adolescent population has been steadily rising. According to the previous studies, the rs1801282 (Pro12Ala) polymorphism in the PPARγ gene is connected to the development of obesity, hypertension, insulin resistance, hyperlipidaemia, and other metabolic complications of type 2 diabetes. Data collection was undertaken as part of the COPAT project (Childhood Obesity Prevalence and Treatment). A sample of 2246 Czech adolescents aged 13-17 was genotyped using RT-PCR and subjected to further anthropometric and clinical measurements, and biochemical testing. Data on their nutritional intake and dietary habits were collected as well. A randomly selected subgroup of 735 individuals was further tested for serum fatty acids ratios. Our goal was to 1) determine the effect of the rs1801282 polymorphism in the PPARγ gene on the anthropometric, clinical and biochemical parameters, 2) determine the effect of the interaction between the rs1801282 polymorphism in the PPARγ gene and obesity or overweightness on the observed parameters. The frequency of Ala allele in our sample was 15%. Ala allele was associated with lower fasting c-peptide (p=0,006) and fasting insulin levels (p=0,035). In obese Ala boy carriers higher levels of phospholipid and triacylglycerol...

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Bainová, Zuzana. "Polymorfismus heterodimerů TLR2/TLR1 a TLR2/TLR6 u inbredních linií myši domácí odvozených z přirozených populací." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-323626.

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Contrary to the classical mouse inbred strains with unnatural genetic variability, wild-derived strains offer a more suitable model for evolutionary immunology. Toll-like receptors (TLRs) belong to initial detectors of invading pathogens. Although TLRs recognise conserved structures they were shown to be polymorphic. This polymorphism is associated with various diseases. In my thesis, I describe variability of Tlr1, 2 and 6 in 24 inbred strains derived from two subspecies of house mouse (Mus m. musculus and M. m. domesticus). These Tlrs exhibit different levels in variability among the strains. In Tlr1 the polymorphic sites are spread along the whole exodomain. Tlr6 is quite conserved (a lower amount of substitutions located far from the binding region and with minor modifications in the amino acid residue properties). Tlr2, on the contrary, contains some substitutions with substantial alternations of residue properties that are located within or nearby the binding region and the subspecies differ at these sites. All alleles of M. m. domesticus and M. m. musculus, except for Tlr1 PWD, Tlr2 STAIL, are phylogenetically separated. The strains and the subspecies vary in the production of IL-1β, IL-12 a NO after stimulation by TLR1, 2 and 6 ligands. This trend is, however, presumably influenced by the effect of...

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Hovhannisyan, Milena. "Polymorfismus rs v genu PPARG ve vztahu ke koncentraci a složení mastných kyselin u českých adolescentů." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-415786.

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The prevalence of obesity and type 2 diabetes in the adolescent population has been steadily rising. According to the previous studies, the rs1801282 (Pro12Ala) polymorphism in the PPARG gene is connected to the development of obesity, hypertension, insulin resistance, hyperlipemia, and other markers of type 2 diabetes. Data collection was undertaken as part of the COPAT project (Childhood Obesity Prevalence and Treatment). A sample of 2246 Czech adolescents aged 13-17 was genotyped using RT-PCR. 735 individuals from this sample were subjected to further anthropometric and clinical measurements, and biochemical testing. Data on their nutritional intake and dietary habits were collected as well. Our goal was to 1) determine the effect of the polymorphism on the anthropometric, clinical and biochemical parameters, 2) determine the effect of the genotype-phenotype interaction on the lipid spectra. We measured the frequency of Ala allele as 15%. Ala allele was associated with lower fasting c-peptide (p=0,006) and fasting insulin levels (p=0,035). In obese Ala carriers we detected higher levels of phospholipid and triacylglycerol ω-3 polyunsaturated fatty acid than in lean Ala carriers or obese noncarriers. Keywords: PPARγ2, Pro12Ala, fatty acids, obesity, COPAT project

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Steinerová, Michala. "Variabilita genů IGFBP2 a MC4R ve vztahu ke kvalitě masa prasat." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-426834.

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The aim of this diploma thesis was association analysis of single nucleotide polymorphism of IGFBP-2 and MC4R genes in a selected group of Czech Large White pigs with indicators of pork quality. The surveyed traits were pH value, color of meat, electrical conductivity, drip loss, contents of intramuscular fat and fatty acids. The polymorphisms were detected by the PCR and RFLP procedures. The allele and genotype frequencies were calculated and results were statistically evaluated using SAS program. In the case of the IGFBP-2 gene was discovered, that the observed set of animals is marked out by the higher percentage of allele B (99,5 %), the relative frequency of the BB genotype was 99 %, AB 1 % and genotype AA has not been found. For this reason, an association analysis could not be performed. The MC4R gene showed a higher frequency of the G allele (74.52%), with the relative frequency of AA genotypes being 2.88%, AG 45.19% and GG 51.92%. On the basis of the association analysis, statistically significant differences were found in the content of the linoleic acid at significance level of P ≤ 0,05 between genotypes AG×GG and to the drip loss where was the difference between the genotypes AA×GG. No statistically significant differences were found for other meat quality indicators, not even value close to significant difference (P ≤ 0,1).

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Ludikovská, Nicole. "Variabilita genu MC1R u psů plemene flat coated retriever." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-430207.

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A MC1R nonsense mutation, R306ter, has been shown to cause a completely yellow coat color in flat coated retriever. A c.916C>T change in the single exon MC1R gene leads to a change from arginine amino acid to a premature stop codon (R306ter). Using DNA sequencing, the polymorphism was found, and genotype and allele frequencies were determined for a selected set of 50 dogs. The frequency of the heterozygous genotype E / e and the undesirable recessive allele e was higher in the selected group in the Czech Republic than we expected.

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VAŇKOVÁ, Adéla. "Vliv polymorfismu kandidátního lokusu na technologické vlastnosti mléka." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-396073.

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The aim of this diploma thesis was to investigate the influence of milk betacasein locus (CSN2) polymorfic variants on production characteristics and milk technological qualities in Czech Simmental cattle and Holstein cattle. DNA extracted from milk of 702 cows was genotyped using PCR and RFLP methods. The genotypes A1A1, A1A2, and A2A2 had the following distribution within the population studied: A1A1 in 11,40 %, A1A2 in 8,46 %, and A2A2 in 50,14 % cows. The studied milk parameters were the milk yield (kg), protein and fat percentage and protein and fat yield (kg). The statistical evaluation was made STATISTICA 12 program. We found no statisticaly significant influence of CSN2 genotype on the selected milk yields. The results might be biased by the relatively low amount of individuals studied. Research will continue within the QJ1510339 and GAJU028/2019/ Z grant.

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Samaké, Kalifa. "Polymorfismus transkripčního faktoru NF-κB a Toll-like receptoru 2 u produkční populace skotu (Bos taurus L.)." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-393170.

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The broader purpose of the work is to find and interpret polymorphism in the genes of natural immunity of cattle to be used to improve disease resistance. The NGS method on the PacBio platform was applied for the resequencing of the gene for the key receptor of innate immunity TLR2 and two genes coding for the components of the downstream transcriptional factor NF-κB. In the population of 149 bulls of the Czech Simmental breed, 22 polymorphisms were found in the gene NFKB1 (5 new), while in the NFKB2 gene 13 SNP were found (10 new). 21 SNP were found in the TLR2 gene (3 new). Of the 56 found polymorphisms, 6 SNPs were nonsynonymous. One SNP leads to a change R474G in the NFKB1 product and five to changes E63D, R152Q, I211V, R563H and H665Q in the protein TLR2. Knowledge of the haplotypes facilitated the development of individual genotyping reactions. In TLR2, a high number of haplotypes was detected, both from the PacBio reads and the statistical reconstruction. In addition, two clusters of haplotypes were ditinguished inTLR2, possibly due to diversifying selection or introgression. The knowledge of genetic diversity in the population allows for the planned association studies with health data. Localization in functional domains allow to define the change with the greatest effect, in particular...

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JANDOVÁ, Linda. "Možná asociace polymorfismů v genu pro dopaminový receptor D2 (\kur{DRD2}) s lidským chováním." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-394613.

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This study is focused on the dopamine D2 receptor and the linkage between TaqIA and - 141C Ins/Del polymorphisms in DRD2 gene and novelty seeking behaviour, as well as the linkage between Val158Met polymorphism in COMT gene and novelty seeking behaviour. Novelty seeking behaviour is characterized as a personality trait with a tendency to look for novel stimulation and extravagances at any cost of legal, physical and social risk to reward cues. DRD2 and COMT genes are associated with the function of dopamine, which is essential for motoric function and reward-motivated behaviour. Therefore, the aim of this study is to ascertain the potential linkage among the three studied polymorphisms and novelty seeking behaviour.

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Máčalík, Tomáš. "Problematika rekrystalizace účinných látek v léčivých přípravcích." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-396984.

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1 Abstract Charles University in Prague, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Consultant: PharmDr. Ondřej Holas, Ph.D. Student: Tomáš Máčalík Title of Thesis: Problematics of active compounds recrystalisation in pharmaceutical preparations The topic of the thesis is the recrystallization of active substances in pharmaceutical preparations. The aim of this work is to demonstrate this phenomenon on a real particular pharmaceutical product, in which it has occurred. It includes a detailed description of the manufacturing process including all mandatory conditions, possible critical points, investigations and measures taken. The thesis consists of two parts. The first describes in details the current state of knowledge. It is based on the findings of the available literature on the subject. The basic concepts of crystallization and polymorphism are explained in detail, which form the basis for the subsequent case study. The case study includes a detailed description of a specific medicinal product. Subsequently, it's production, good manufacturing practice and gradual investigation of the discovered technological incompatibility are explained step by step. The result of the work points out that the issue of recrystallization and polymorphism is very closely related to...

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Dolnáková, Lenka. "Štúdium variability sekvencie v géne MC1R u rôznych druhov zvierat." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-427667.

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Colouration is an important phenotypic trait of animals and plays a substantial role in the characteristics of individual animal species. The MC1R gene has an essential role in colouration. This thesis is focused on the study of sequence variability in this gene and subsequent phylogram formation in selected species, which are: Bos Primigenius Taurus, Sus Scrofa, Equus Caballus and Canis Lupus Familiaris. There were custom primers designed in Oligo for the selected exon sequence. Based on se-quencing, known polymorphisms in Canis Lupus Familiaris and Equus Caballus were detected. In Canis Lupus Familiaris sequence, there was a 790 A>G polymorphism. All of N sequenced samples (N = 10) carried the E (790AA) allele. In Equus Caballus se-quence, there was a 901 C>T polymorphism, with representatives of all genotypes nEE(901CC) = 2, nEe(901CT) = 2, nee(901TT) = 6. The thesis also provides a comprehensive overview of the phylogenetic tree for-mation process. For phylogenetic analysis, there were added sequences from other ani-mal species, gained from freely available genomic databases. The reconstruction took place in the MEGA X program with selected distance and sign methods. Only nodes with bootstrap support ≥ 70 could be interpreted. JC69 and HKY85 models were cho-sen as substitution models. The choice of models was statistically supported by testing likelihood in the Model Generator with P < 0,0001 support. The trees obtained by par-ticular methods were compared by the Pearson correlation coefficient.

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Hronová, Karolína. "Polymorfismus genů účastnících se v metabolismu léčiv jako potencionální cíl prevence závažných komplikací léčby u novorozenců a dětí." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-389807.

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Univerzita Karlova 1. lékařská fakulta Studijní program: Biomedicína Studijní obor: Preventivní medicína MUDr. Karolína Hronová Polymorfismus genů účastnících se v metabolismu léčiv jako potenciální cíl prevence závažných komplikací léčby u novorozenců a dětí Polymorphism of drug metabolizing enzymes as a potential target of prevention of serious treatment complications in neonates and infants Disertační práce- ABSTRAKT V ANGLICKÉM JAZYCE Školitel: prof. MUDr. Ondřej Slanař, Ph.D. Konzultant: MUDr. Pavla Pokorná, PhD. Praha, 2018 Abstract Background and aims: The safety of analgosedative drugs includes drug interactions, adverse effects, withdrawal syndrome and drug dependence are factors that significantly affect morbidity and mortality. Its prevention is critical for quality improvement of care in paediatric patients. The aim of the thesis was to evaluate the prediction of efficacy and safety of analgosedative drugs sufentanil, midazolam, tramadol and valproic acid in neonates and children based on the occurrence of selected pharmacogenetic biomarkers. The incidence of drug interactions of phenobarbital with other analgosedative drugs has also been evaluated. Methodology: The thesis is based on two studies conducted on Intensive and Resuscitation Care Unit of the Clinic of Paediatric and Adolescent...

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Staroveská, Marieta. "Detekce polymorfismu v genu MDR1 u ovčáckých a honáckých psů." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-362299.

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This thesis is focused on polymorphism of MDR1 gene and related drug resistance. Resistance is caused by deletion of four nucleotids, that resulting in a frame shift and synthesis of nonfunctional transport of P-glycoprotein. The text describes a polymorphism of MDR1 (ABCB1) gene, which results in reduced resistance to drugs belonging to the group of macrocyclic lactones. It also describes inheritance of this phenomenon and it deals with the detection of mutation using PCR (polymerase chain reaction) and by fragmentation analyses. A review of literature study is a form of research solely from scientific publications. 128 dogs were included into the own analysis. The results confirmed that Collies had the highest presence of deletions (29,73 %) with a high number of carriers in the study population of dogs (54,05 %). The percentage of affected individuals in the breed of Australian Shepherd and Sheltie was significantly lower (7,32 % and 6 %), but the percentage of carriers were also high in both Australian Shepherds (34,14 %) and the breed Sheltie (48 %).

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Civáňová, Kristina. "Methods of detection and analysis of single nucleotide polymorphisms in genes influencing the meat performance in pigs." Doctoral thesis, 2005. http://www.nusl.cz/ntk/nusl-92790.

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Dissertations / Theses on the topic 'Polymorfismus'

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