Relevant bibliographies by topics / Polymorphic DNA

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Polymorphic DNA'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Polymorphic DNA"

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Subramanian, V., S. Gurtu, R. C. Nageswara Rao, and S. N. Nigam. "Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay." Genome 43, no. 4 (2000): 656–60. http://dx.doi.org/10.1139/g00-034.

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Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic DNA (RAPD) assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits.Key words: Arachis hypogaea L., RAPD, DNA polymorphism, oligonucleotide, random primers.
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González, Juan Manuel, and Esther Ferrer. "Random amplified polymorphic DNA analysis in Hordeum species." Genome 36, no. 6 (1993): 1029–31. http://dx.doi.org/10.1139/g93-137.

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Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.
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Sakakibara, Stacey M., and John E. Carlson. "DNA FINGERPRINTING IN RHODODENDRONS USING RANDOM AMPLIFIED POLYMORPHIC DNA." HortScience 31, no. 3 (1996): 323g—324. http://dx.doi.org/10.21273/hortsci.31.3.323g.

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Random amplified polymorphic DNA (RAPD) markers were evaluated for use in DNA fingerprinting of commercial Rhododendron cultivars. DNA was isolated from Rhododendron leaves and subjected to PCR amplification with single primers, 10 nucleotides in length, and of arbitrary sequence. Amplification products were visualized by agarose gel electrophoresis and ethidium bromide staining. Fingerprints were readily identifiable for a number of cultivars, and a high level of polymorphism was observed among clones of 10 rhododendron varieties. The technique was consistently reproducible in different trials using the thermocycler, between different thermocyclers, and using different DNA isolation from the same plant. This method will be applied to large-scale fingerprinting of Rhododendron cultivars and for distinguishing material propagated in tissue culture.
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MacPherson, J. M., and A. A. Gajadhar. "Random amplified polymorphic DNA." Parasitology Today 8, no. 7 (1992): 235. http://dx.doi.org/10.1016/0169-4758(92)90120-q.

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Echt, C. S., L. A. Erdahl, and T. J. McCoy. "Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa." Genome 35, no. 1 (1992): 84–87. http://dx.doi.org/10.1139/g92-014.

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Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.Key words: random amplified polymorphic DNA, polymerase chain reaction amplification, genome mapping, restriction fragment length polymorphism, Medicago sativa.
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Khandka, D. K., A. Nejidat, and A. Golan-Goldhirsh. "Polymorphism and DNA markers for asparagus cultivars identified by random amplified polymorphic DNA." Euphytica 87, no. 1 (1996): 39–44. http://dx.doi.org/10.1007/bf00022962.

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Omanwar, S., R. K. Singh, G. Butchaiah, and J. R. Rao. "DNA polymorphism in Tiypanosoma evansi isolates defined by randomly amplified polymorphic DNA-PCR." Veterinary Record 148, no. 8 (2001): 244–46. http://dx.doi.org/10.1136/vr.148.8.244.

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Carvalho, M., M. Matos, and V. Carnide. "Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits." Horticultural Science 41, No. 4 (2014): 175–84. http://dx.doi.org/10.17221/21/2014-hortsci.

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  In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivars using morphological and flavour traits are largely unsuccessful, due to environmental influences. The genetic similarity of ten highbush blueberry cultivars was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers from fruits and leaves. The DNA concentrations obtained in fruits and leaves were very similar and the band profiles observed in the two tissues were analogous with both molecular markers. RAPD analysis generated 144 bands, of which 112 were polymorphic (77.8%) in fruits and 141 bands of which 118 were polymorphic (83.7%) in leaves. In fruits, ISSR analysis produced 151 bands of which 127 were polymorphic (84.1%) and in leaves it produced 148 bands with 127 being polymorphic (85.8%). Dendrogram and principal coordinates analysis (PCO) analysis using the both markers results were concordant and a clear division of the types of highbush blueberry cultivars (northern and southern) into two distinct groups was verified.    
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Galovic, Vladislava, Snezana Mladenovic-Drinic, Drazen Jelovac, and Julijana Navalusic. "Application possibilities of AFLP fingerprinting technique in maize DNA profiling and plant variety protection." Genetika 36, no. 2 (2004): 133–42. http://dx.doi.org/10.2298/gensr0402133g.

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As a contribution to DUS testing within the system of protection of plant breeders' rights (PBR), the AFLP molecular system has been used in this study to produce DNA fingerprinting profiles. DNA polymorphism and genetic distance of nine agronomicaly important maize genotypes has been investigated using the AFLP technique. Two specific adapters, two preselective primers and twenty selective primers were utilized for DNA amplification. The selective primers were GC rich, each having a 3-mer selective sequence at 3' termini. Ten double stranded primer combinations were made out of the twenty primers but only five of them turned out to be reliable. Out of 253 amplified DNA fragments, 177 were polymorphic (70%). The CGA/GAG (B) primer combination has proved to be the most polymorphic (44 polymorphic fragments have been recorded) revealing the polymorphism rate of 81.5%. Genotypes g1 and g7 were most distinct (GD=55% and GD=79%, respectively) and genotypes g1. g4 and g8 were closest (GD=55% in all cases). The paper discusses possible uses of AFLP DNA profiling technique to achieve a unique fingerprinting pattern of agronomicaly important maize genotypes.
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Levi, Amnon, and Claude E. Thomas. "DNA Markers from Different Linkage Regions of Watermelon Genome Useful in Differentiating among Closely Related Watermelon Genotypes." HortScience 42, no. 2 (2007): 210–14. http://dx.doi.org/10.21273/hortsci.42.2.210.

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A genetic linkage map was previously constructed for watermelon using a wide testcross population [{Plant Accession Griffin 14113; Citrullus lanatus var. citroides (L.H. Baiely) Mansf.} × the watermelon cultivar New Hampshire Midget; NHM {(Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus)} × United States Plant Introduction (PI) 386015 {Citrullus colocynthis (L.) Schrad.}]. One-hundred forty-six markers [randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) markers] unique to NHM and representing different linkage groups on the map were tested for polymorphism among 24 watermelon cultivars limited in genetic diversity. Five (9.4%) of 53 RAPD, six (40.0%) of 15 ISSR, 30 (81.0%) of 37 AFLP, and 33 (80.5%) of 41 SRAP markers tested produced polymorphism among the 24 cultivars. The polymorphic markers used in this study are scattered throughout the watermelon genome. However, a large number (19 of the 30) of AFLP markers clustered on one linkage group on the map. The SRAP markers proved to be most effective in producing polymorphism and in representing different linkage regions of watermelon genome. The polymorphic markers represent all 10 large linkage groups and five of the nine small linkage groups (altogether 15 of 19 linkage groups) of the genetic linkage map constructed so far for watermelon. These polymorphic markers can be useful in DNA fingerprinting of cultivars, in testing seed purity of breeding lines, and in identifying triploid (seedless) hybrid watermelons derived from crosses between closely related tetraploid and diploid lines.
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Dissertations / Theses on the topic "Polymorphic DNA"

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Gray, Ian Christopher. "Polymorphic tandemly repeated sequences in human DNA." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/34415.

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Tandemly repeated tracts of DNA are a ubiquitous feature of eukaryote genomes. One class of tandem repeats, 'minisatellites', have been shown to be highly variable both in overall length and in the internal arrangement of variant versions of the repeating unit along the array. Consequently, both length and internal variation at these loci can be exploited to generate individual-specific profiles of use in forensic science and the establishment of family relationships. Recently it has been demonstrated that short dinucleotide repeats, or 'microsatellites', and other simple tandem repeat arrays can also show length variation. This work describes the isolation and characterization of simple tandem repeat arrays, and their application in a forensic science case. The evolutionary persistence of variability at tandem repeat loci is also explored. Simple tandem repeats isolated were frequently associated with other tandem repeats and interspersed repetitive elements, a phenomenon previously described for minisatellites and perhaps indicative that certain genomic regions show relaxed fidelity in the maintenance of large-scale DNA structure, allowing tandem array expansion and retroposon insertion. Although variability is low relative to minisatellites, microsatellites, owing to limited length, can readily be amplified from highly degraded DNA using the polymerase chain reaction. Consequently it was possible to identify positively the skeletal remains of a murder victim by comparing microsatellite profiles of the skeleton with those of the presumptive parents. Comparative studies of microsatellite and minisatellite loci between man and other primates indicate that in evolutionary terms, the variable state is reasonably persistent at microsatellite loci, whereas highly variable minisatellites show extreme evolutionary transience. Such transience was also demonstrated for two large 'midisatellite' loci, suggesting that highly variable tandem repeat loci are extremely unstable and transient, whereas lower variability leads to evolutionary persistence of the variable state.
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Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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Papadopoulos, Sarantos. "Untersuchungen genomischer Veränderungen von Mammakarzinomzellen mittels Random amplified polymorphic DNA." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962689114.

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Papadopoulos, Sarantos. "Untersuchungen genomischer Veränderungen von Mammakarzinomzellen mittels Random Amplified Polymorphic DNA." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2001. http://dx.doi.org/10.18452/14631.

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Im ersten Teil der Arbeit wurde die random amplified polymorphic DNA (RAPD) Methode für die Anwendung humaner DNA optimiert, indem die Konzentrationen der einzelnen Agentien und das thermische Profil der PCR-Reaktion verändert wurden. Des weiteren wurde der Einfluss von Polymerasen und PCR-Maschinen auf die RAPD, insbesondere auf die Erweiterung des Spektrums der Amplimere und die Reproduzierbarkeit der Reaktionen untersucht. Unsere Untersuchungen haben gezeigt, dass die RAPD eine sehr robuste und reproduzierbare Methode ist. Im zweiten Teil wurden qualitative und quantitative Unterschiede zwischen DNA von Brustkrebszellen und DNA von Leukozyten detektiert. Die dazu benutzten Primer basieren auf Sequenzen die in den Mechanismen der Tumorgenese involviert sind. Unsere Studie hat gezeigt, dass random priming in der Abschätzung von genomischen Schäden im Brustkrebs sehr nutzbar sein kann.<br>In the first part of this work, we have optimized random amplified polymorphic DNA (RAPD) for the use of human DNA in altering the concentration of the reaction components and the steps of the thermal profile in the polymerase chain reaction, by testing a large number of polymerases and multiple combinations with respect to their ability to increase the spectrum of amplimers and by examining the performance of various thermocyclers. We conclude that RAPD is a robust and reproducible method that could prove very useful for scientists and physicians. In the second part we used primers that were designed by choosing sequences involved in the development of DNA mutations, to successfully detect qualitative and quantitative differences between breast cancer DNA/normal DNA pairs. Our study showed that random priming proves very useful for assessing genomic damage in breast cancer.
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Robert, Florence. "Typage de "Candida albicans" par Random Amplified Polymorphic DNA (RAPD)." Paris 5, 1992. http://www.theses.fr/1992PA05P198.

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Campos, Lázara Pereira. "Genome relationships among Lotus species based on random amplified polymorphic DNA (RAPD)." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56888.

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The usefulness of RAPDs (Random Amplified Polymorphic DNA) to distinguish among different taxa of Lotus was evaluated. The following species were included: L. corniculatus, L. tenuis, L. alpinus, L. japonicus, and L. uliginosus. Several accessions for each species were studied. Following DNA extraction, amplification reactions were performed in a Hybaid DNA Thermal Cycler, and the product visualized according to a standard procedure. Twenty primers were used for each species/accession. Clear bands and several polymorphisms were obtained for all primers. A phenogram was drawn based on the genetic distance among the species. L. alpinus appears as the most distant species from L. corniculatus, followed by L. uliginosus, L. tenuis, and L. japonicus. With the exception of L. alpinus, these findings are in agreement with previous experimental studies in the L. corniculatus group. The use of a greater number of primers and increased number of species may provide a greater resolution of the systematics of these taxa.
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Halldén, Christer. "Characterization and use of a multiplex PCR-based system random amplified polymorphic DNA /." Lund : Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/68945134.html.

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Wei, Ling. "Identification of DNA Markers in Triticum aestivum-Aegilops caudata Additions Lines by Randomly Amplified Polymorphic DNA (RAPD) Technology." DigitalCommons@USU, 1995. https://digitalcommons.usu.edu/etd/4543.

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The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T. aestivum X Ae. caudata and Ae. caudata, which was absent in all six addition lines. Each of these two primers produced a chromosome marker that could be tentatively located to the chromosome CA of Ae. caudata. OPJ-02, OPD-12, OPD-02, OPJ-12, OPD-20, and OPJ-14 produced a marker each for CB, CC, CD, CE, CF, and CG, respectively. OPJ-09 produced C-genome chromosome-specific RAPD markers. Also, OPC-05 and OPJ-19 produced RAPDs from both wheat and Ae. caudata genomes.
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Maufrand, Remy. "Linkage analysis of avirulence in Phytophthora infestans using random applied polymorphic DNA markers." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243431.

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Golembiewski, Robert Craig. "Identification and characterization of creeping bentgrass using randomly amplified polymorphic DNA (RAPD) markers /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487942739809001.

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Books on the topic "Polymorphic DNA"

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Hilton, Anthony Craig. Randomly amplified polymorphic DNA analysis of salmonella & campylobacter. University of Birmingham, 1996.

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Gratton, Wayne Stéphane. Metal analysis and random amplified polymorphic DNA characterization of jack pine (Pinus banksiana) populations from the Sudbury region. Laurentian University, Department of Biology, 1998.

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Munthali, Mathildah T. The use of random amplified polymorphic DNA for identifying natural and tissue-culture induced variation in beet (beta vulgaris L.). University of Birmingham, 1992.

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Mitchell, John Edward. Structural polymorphism in repeated DNA. University of Portsmouth, School of Biological Sciences, 1995.

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Wyandt, Herman E. Human Chromosome Variation: Heteromorphism and Polymorphism. Springer Science+Business Media B.V., 2012.

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Meeting, Arbeitsgemeinschaft für Gen-Diagnostik. DNA-Polymorphism in forensic and medicine: 4th Annual Meeting 1988, Arbeitsgemeinschaft für Gen-Diagnostik e.V. Edited by Driesel Albert J, Henke J, and Kömpf J. Hürtig Buch Verlag, 1990.

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NATO Advanced Research Workshop on DNA Polymorphisms as Disease Markers (1990 London, England). DNA polymorphisms as disease markers. Plenum Press, 1991.

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Single nucleotide polymorphisms: Methods and protocols. 2nd ed. Humana, 2009.

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International Bioethics Seminar (7th 2000 Fukui-shi, Japan). Bioethics and the impact of genomics in the 21st century: Pharmacogenomics, DNA polymorphism and medical genetics services = 21-seiki ni muketa atarashii genomikkusu no j̄idai no rinri : DNA takei to iden igaku sābisu o megutte : proceedings of the seventh International Bioethics Seminar in Fukui. Edited by Fujiki Norio 1928-, Macer, Darryl R. J. 1962-, Sudo Masakatu 1938-, and Eubios Ethics Institute. Eubios Ethics Institute, 2001.

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Sniegowski, Paul D. Transposable elements and polymorphic inversions in Drosophila melanogaster. 1993.

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Book chapters on the topic "Polymorphic DNA"

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Galvão, Lúcia Maria da Cunha, and Eliane Lages-Silva. "Randomly Amplified Polymorphic DNA (RAPD)." In Springer Protocols Handbooks. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_10.

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Dassanayake, Ranil S., and Lakshman P. Samaranayake. "Randomly Amplified Polymorphic DNA Fingerprinting." In PCR Protocols. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_23.

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Micheli, M. R., R. Bova, and E. D’Ambrosio. "Random Amplified Polymorphic DNA Assay." In Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60441-6_9.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology Manual. Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_27.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-017-6951-8_3.

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Mischke, D., R. Wanner, and B. P. Korge. "Polymorphic Keratins as Detected by PCR and SSCP." In Methods in DNA Amplification. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2530-1_4.

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Babu, Kantipudi Nirmal, Muliyar Krishna Rajesh, Kukkumgai Samsudeen, et al. "Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-767-9_10.

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Babu, Kantipudi Nirmal, Thotten Elampilay Sheeja, Divakaran Minoo, et al. "Random Amplified Polymorphic DNA (RAPD) and Derived Techniques." In Methods in Molecular Biology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0997-2_13.

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Novick, G. E., T. Gonzalez, J. Garrison, et al. "The use of polymorphic Alu insertions in human DNA fingerprinting." In DNA Fingerprinting: State of the Science. Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-8583-6_26.

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Ott, Jurg. "Estimating Crossover Frequencies and Testing for Numerical Interference with Highly Polymorphic Markers." In Genetic Mapping and DNA Sequencing. Springer New York, 1996. http://dx.doi.org/10.1007/978-1-4612-0751-1_4.

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Conference papers on the topic "Polymorphic DNA"

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Chan, Vivian, V. W. S. Liu, A. C. K. Wong, and T. K. Chan. "DNA POLYMORPHISMS IN OR LINKED TO THE FACTOR VIII GENE IN CHINESE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644049.

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78 unrelated X chromosomes from Southern Chinese (56 normal and 22 haemophiliac) were studied. DNA was restricted by Bel I, Bgl I or Taq I and hybridized to 3' factor VIII:C cDNA probe (5 kb, Chiron) or St 14.1 probe(3 kb, Oberle &amp;Mandel) by standard techniques. The intragenic Bel I polymorphic site was positive in 82%, while Bgl I polymorphic site was positive in all. Thus, 29.5%(2 x×0.82 × 0.18) of Chinese females carried the Bel I polymorphism. Asto the Taq I polymorphism in the closely linked DXS52 DNA segment, the incidences for the various alleles were :System I - allele (3) 10.2%, (4) 2.6%, (5) 2.6%,(6) 17.9%, (7) 21.8% and (8) 44.9% System II - α a allele 56%, 6 allele 44%. Approximately 80% of females were heterozygous for two different alleles. Hence the Bel I and Taq I polymorphisms can be used to track the defective factor VIII gene for carrier detection and prenatal diagnosis. Furthermore, their frequencies in the Chinese are different from those previously reported in other ethnic groups.
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia, and C. Peschle. "POLYMORPHIC SITES IN FACTOR X GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several polymorphic restriction sites after digestion with EcoRI, Hind III and Pvu II.The restriction pattern obtained after Hind III digestion showed two bands of 7.3 and 6.0 Kb, while in two families an additional 7.6 Kb band was observed. Genomic DNA digested with EcoRI showed a 7.1 and 5.1 Kb fragments, and also a 6.6 Kb band with a 10% f requency.After DNA digestion with Pvu II 5.6, 2.7 and ∼1.0 Kb bands were observed. In three unrelated subjects we observed an additional 3.0 Kb fragment, in two other subjects a 3.5 kb band. Interestingly hybridization with a 178 bp cDNA subclone allowed to map the polymorphic sites in a the 3’ region of the gene locus.
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Chelu, Cristina, Carmen Varlam, Gheorghe Titescu, and Gallia Butnaru. "Diversitatea moleculară a două ecotipuri de Datura inoxia provenite din vestul şi estul României." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.03.

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Molecular Diversity of two Ecotypes of Datura inoxia Originating from Western and Eastern Romania. To characterize genomic variation among genotypes, we have performed RAPD analysis using ten random primers. The results yielded 88 bands out of which 39 were polymorphic. The primers US1 and US7 showed 87.71% and 72.72% polymorphism respectively. The least polymorphism was shown by primer US9 (12.50%). The primer US15 did not produce any bands suggesting the absence of matching sequences in the genomic DNA. The dendrogram classified ecotypes into two clusters (A and B); cluster B possess three sub-clusters: B1 - Socodor 2; B2 - Flamura 1 and Flamura 2, and B3 - Flamura 3. Overall, the values of genetic similarity between ecotypes were low pointing out their particular origin and “evolution”.
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Suzuki, N., A. Iizuka, T. Nagao, Y. Nakahori, M. Yamada, and Y. Nakagome. "CARRIER DETECTION OF HEMOPHILIA A BY DNA ANALYSIS IN AFFECTED JAPANESE FAMILIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644008.

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Several DNA probes have been isolated to detect Factor VIII gene and a DNA segment which locates veryclose to the gene. They have been successfully used to detect carriers and patients of hemophilia A.We analyzed DNA samples of Japanese population to see whether these probesare also useful for carrier detection of hemophilia A in affected Japanese families, since the size and frequency of allelic fragments detected by a DNA probe are sometimes different in various ethnic groups.A probe of St14 (DXS52) is thought to be one of the best probes for such analysis in Caucasian population because it detects very polymorphic DNA fragments containing a minisatellite. When Taq I digests of Japanese DNA samples were hybridized with Stl4, several DNA fragments with a range from 1.7 kb to 5-5 kb were detected, where .at least 6 fragments were polymorphic. A notable difference between Japanese and Caucasian was that a band of 5-5 kb was variable in Japanese while it was constant in Caucasian. We have so far detected 10 alleles, and about 60% of Japanese women were heterozygous. Using these informationsabout Japanese population, we could detect carriers in several families. Other RFLPs data are necessary to increase information content. Similar studies arein progress using different probes i.e. an extragenic probe ; DX13/Bgl II, and two intragenic probes ; exon 14-26/Bcl I and exon 26/Bgl I. We thank Mandel J.L., Strasbourg, Davies K., Oxford and Genetics Institute, Cambridge for probes.
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Graham, J. B., D. B. Lubahn, J. D. Kirshtein, et al. "THE “MALMO“ EPITOPE OF FACTOR IX: PHENOTYPIC EXPRESSION OF THE “VIKING“ GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643566.

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The epitope of a mouse monoclonal AB (9.9) which detects a Factor IX (F.IX) polymorphism in the plasma of normal persons (PNAS 82:3839, 1985) has been related to not more than 6 AA residues of F.IX by recombinant DNA technology. The same 6 residues define Smith’s polymorphic epitope (Am. J. Human Genet. 37:688, 1985 and in press). This region of F.IX contains the alanine:threonine dimorphism at residue 148 first suggested by McGraw et al. (PNAS 82: 2847, 1985) and established by Winship and Brownlee with synthetic DNA oligomers (Lancet in press). Using synthetic DNA probes, we have found that the DNA difference between positive and negative reactors to 9.9 is whether base pair 20422, the first pair in the codon for residue 148, is A:T or G:C. We can conclude that 9.9 reacts with F.IX containing threonine but not alanine at position 148.The F.IX immunologic polymorphism-whose epitope we are referring to as “Malmo”-is, not surprisingly, in strong linkage disequilibrium with two F.IX DNA polymorphisms, TaqI and Xmnl. The highest frequency of the rarer Malmo allele in 6 disparate ethnic groups was in Swedes (32%); a lower frequency (14%) was seen in White Americans whose ancestors came overwhelmingly from the Celtic regions of the British Isles; it was at very low frequency or absent in Black Americans, East Indians, Chinese and Malays. A maximum frequency in Swedes and absence in Africans and Orientals suggest that the transition from A:T to G:C occurred in Scandinavia and spread from there. The history of Europe and America plus the geographical distribution of the rare allele lead us to suggest that this locus might be designated: “the Viking gene”.
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Oclarit, Jose M., and Nathaniel L. Hepowit. "DNA Amplicons using Arbitrary Primers Distinguish Polymorphic Loci Among Mangrove Thraustochytrid Genomes." In OCEANS 2007 - Europe. IEEE, 2007. http://dx.doi.org/10.1109/oceanse.2007.4302194.

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Eriksson, John, Charlotta Löfström, and Peter Rådström. "Randomly amplified polymorphic DNA (RAPD) typing of Salmonella Senftenberg in animal feed production." In Fifth International Symposium on the Epidemiology and Control of Foodborn Pathogens in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-538.

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Liu, Haiying, Guie Wang, Xiangying Meng, and Xiuli Wang. "Genetic Variation in Six Oratosquilla oratoria Populations Revealed by Random Amplified Polymorphic DNA (RAPD) Markers." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5516354.

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Golovatskaya, A. V., and S. Z. Guchetl. "THE CERTIFICATION OF SUNFLOWER LINES FROM THE COLLECTION OF THE DON EXPERIMENTAL STATION OF V.S. PUSTOVOIT ALL-RUSSIAN RESEARCH INSTITUTE OF OIL CROPS BY USING DNA MARKERS." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-39-43.

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The aim of this research was to develop molecular genetic passports of sunflower lines from the collection of the Don experimental station of V.S. Pustovoit All-Russian Research Institute of Oil Crops based on polymorphic fractions of microsatellite DNA. We used 17 lines as a research material. We used 12 pairs of primers for genotyping. We found that the ORS 559 locus was monomorphic for these samples. The rest of the loci had from 2 to 4 alleles. The average number of alleles per locus was 2.75, PIC – 0.49, the effective number of alleles – 2.16. The analysis of the DNA profiles of the lines showed the individuality of the allelic composition of each of them. The analysis of the genetic relations between the lines showed that the studied lines were divided into two groups, with a genetic distance between them of 5.9.
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Ifah, Arini Al, Endang Yuniastuti, and Parjanto. "Analysis of breadfruit plant diversity (Artocarpus altilis P.) by random amplified polymorphic DNA (RAPD) in DIY." In THE 8TH ANNUAL BASIC SCIENCE INTERNATIONAL CONFERENCE: Coverage of Basic Sciences toward the World’s Sustainability Challanges. Author(s), 2018. http://dx.doi.org/10.1063/1.5062802.

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Reports on the topic "Polymorphic DNA"

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Cai, H., K. Kommander, P. S. White, and J. P. Nolan. Flow cytometry-based DNA hybridization and polymorphism analysis. Office of Scientific and Technical Information (OSTI), 1998. http://dx.doi.org/10.2172/663513.

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Amzeri, Achmad, Kaswan Badami, and Gita Pawana. Inheritance of resistance to downy mildew (Peronosclerospora maydis) in crossing of Madura Maize Plant (Zea mays L.). Innovative Scientific Information & Services Network, 2019. http://dx.doi.org/10.21107/amzeri.2019.1.

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Hybridization of Back cross is one method to get varieties that are resistant to downy mildew. The purpose of this study was to obtain information on inheritance characteristics of downy mildew resistance. This research was conducted at the experiment center of Agro-Technology Study Program of Agriculture Faculty, University of Trunojoyo Madura. Research of Assessment of resistance to Downy Mildew used a randomized block design with 18 treatments (P1, P2, F1, F2, BC1P1 and BC1P2 in three sets of crosses, namely LGL x Mdr-3, T12 x Mdr-1 and E02 x Mdr-2) and three replications so there were 54 experimental units. Identification of polymorphic RAPD markers for endurance to downy mildew through Bulk Segregant Analysis (BSA) was done by amplifying the DNA in the resistant pool and susceptible pool. The random primers used were 120 primers from 6 operon groups, namely OPA, OPB, OPC, OPD, OPF and OPG. The results showed that the inheritance pattern of maize genetic resistance to downy mildew followed a segregation pattern of 3:1 with a degree of dominance between -1 and 0, and was controlled by incomplete partially negative dominant gene. OPC-07 was a marker that was linkage close to the resistance to downy mildew with a genetic distance of 1.9 cM.
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Lee, K. Y., and M. Chan. The two polymorphs of N-DNAT, a high nitrogen molecule. Office of Scientific and Technical Information (OSTI), 1995. http://dx.doi.org/10.2172/102145.

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