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Journal articles on the topic 'Polymorphic marker'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Shankar C., Abida P. S., Jiji Joseph, Rose Mary Francies, Deepu Mathew, and Beena R. "Evaluation for Parental Polymorphism and Identification of Microsatellites Linked to Drought Tolerance in Rice (Oryza sativa L.)." International Journal of Plant & Soil Science 35, no. 22 (2023): 275–84. http://dx.doi.org/10.9734/ijpss/2023/v35i224134.

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Aims: To identify the ready-to-use polymorphic microsatellite markers associated with drought tolerance for marker-assisted backcross breeding through a polymorphism survey between Jyothi and Chuvannamodan rice varieties. Place and Duration of Study: Centre for plant biotechnology and molecular biology, Thrissur, Kerala, India, during January to May 2023. Methodology: The genomic DNA of Jyothi and Chuvannamodan was isolated by following CTAB (Cetyltrimethyl Ammonium Bromide) method with modification. Isolated DNA from both varieties was subjected to PCR amplification using 208 Simp

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KIANI, Ghaffar, and Camellia KATALANI. "Divergence in hybrid rice parental lines detected by RAPD and ISSR markers." Acta agriculturae Slovenica 111, no. 2 (2018): 369. http://dx.doi.org/10.14720/aas.2018.111.2.12.

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<p>Genetic distance between parental lines used in hybrid rice breeding program was estimated based on information from molecular markers data. Sixteen parents (5 CMS, 5 maintainers and 6 restorers) were analyzed with 15 random amplification polymorphic DNA (RAPD) and 20 inter simple sequence repeat (ISSR) marker. Out of 15 RAPD markers, 9 were polymorphic and 79 bands were generated, of which 28 bands were polymorphic (35 %). By using 10 out of 20 ISSR markers 86 bands were detected, of which 35 bands were polymorphic (41 %). Marker index (RAPD = 1.68; ISSR = 1.88) and percent of polymo

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Xie, Qi, Yongyu Zhao, Yumei Liu, Fengqing Han, Wei Liu, and Zhansheng Li. "Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers." Applied Sciences 12, no. 9 (2022): 4754. http://dx.doi.org/10.3390/app12094754.

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To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative and polymorphic SSR markers were screened using broccoli and rapeseed. Out of the 93 SSR marker pairs, 21 pairs were selected and found to have good polymorphism. Each marker pair generated 1 to 10 polymorphic bands with an average of 4.29. The average polymorphism information content (PIC) was 0.41 with a range from 0.16 to 0.95. Six selected marker pairs established the fingerprinting of the 10 accessions and their

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Singh, Shravan Kumar, Prasanta Kumar Majhi, Annamalai Anandan, et al. "Microsatellites based parental polymorphism survey for moisture stress in Rice (Oryza sativa L.) between the parental genotypes HUR-1309 and CR Dhan 801." emergent Life Sciences Research 08, no. 02 (2022): 248–58. http://dx.doi.org/10.31783/elsr.2022.82248258.

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The present investigation was conducted during Rabi 2018-19. The parental polymorphism research was done at the Molecular Drought Breeding Laboratory (Department of Genetics and Plant Breeding), Institute of Agricultural Sciences, BHU, and the Crop Improvement Division, ICAR-NRRI, Cuttack. The purpose of this research was to identify the parental polymorphism markers, analyze their chromosomal distribution, and determine the repetitive motifs. The polymorphic markers will be helpful to introgress the drought tolerant yield QTLs (qDTY) from the donor CR Dhan 801 into the background of HUR-1309,

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Mackill, D. J., Z. Zhang, E. D. Redoña, and P. M. Colowit. "Level of polymorphism and genetic mapping of AFLP markers in rice." Genome 39, no. 5 (1996): 969–77. http://dx.doi.org/10.1139/g96-121.

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Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for gene mapping in plant species. We compared the levels of polymorphism for AFLP, RAPD, and microsatellite markers on 12 japonica and 2 indica rice cultivars. For AFLPs, seven EcoRI and seven MseI primers used in 18 primer combinations generated a total of 529 bands, of which 147 were clearly polymorphic among the accessions. The 21 RAPD primers produced 103 bands of which 43 were polymorphic. For the microsatellite markers the number of alleles per locus ranged from one (1 locus) to six. All marker types gave

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Yadav, Shailendra Singh, and Ashwini A. Waoo. "Molecular Fingerprinting of Indian Medicinal Tree Sara Asoca using RAPD Markers." Biosciences Biotechnology Research Asia 17, no. 4 (2021): 701–5. http://dx.doi.org/10.13005/bbra/2874.

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Saraca asoca is an important medicinal tree facing a serious problem of reduction from its instinctivetenancy in India.Before formulation of conservation strategies for geographical protection of S. asoca genotypes available in India, it is necessary to characterize them. In the current study, the RAPD markers have been utilized effectively for categorization of S. asoca collected from 15 diverse sites of India. An initial experiment on the amplification suitability of genomic DNA samples of four S. asoca was done with 35RAPD primers. Among them only twenty sixproved their efficiency in two ti

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Panigrahi, Kaushik, Puranjaya Panigrahi, Ayesha Mohanty, Purandar Mandal, and Basudeba Satapathy. "Development of new microsatellite markers for DNA fingerprinting pattern of black gram (Vigna mungo L. Hepper) and green gram (Vigna radiate L. Wilckzek)." Genetika 52, no. 3 (2020): 1161–79. http://dx.doi.org/10.2298/gensr2003161p.

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SSR markers are considered to be the most ideal marker for genetic studies because they are multi-allelic, abundant, randomly and widely distributed throughout the genome, co-dominant that could differentiate plants with homozygous or heterozygous alleles, simple to assay, highly reliable, reproducible. Microsatellite markers are highly polymorphic and informative and could be successfully used for genome analysis in black gram & green gram. Microsatellite markers were used to evaluate genetic diversity in 17 indigenous cultivars of pulse crops (11 cultivars of green gram and 6 cultivars o

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Kafkas, Salih, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker. "Genetic Characterization of Hazelnut (Corylus avellana L.) Cultivars from Turkey Using Molecular Markers." HortScience 44, no. 6 (2009): 1557–61. http://dx.doi.org/10.21273/hortsci.44.6.1557.

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Genetic relationships among 18 Turkish hazelnut (Corylus avellana L.) cultivars were investigated using randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) markers. Twenty-five RAPD primers, 25 ISSR primers, and eight AFLP primer pairs generated a total of 434 polymorphic marker loci. The three marker systems were able to differentiate the cultivars. Genetic similarity index values ranged from a high of 0.96 for ‘Kan’ and ‘UzunMusa’ to a low of 0.73 for ‘Yassi Badem’ and ‘Kalinkara’. The genetic relationships were pre

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Hangloo, Sharmishta, Gazi Muhammad Abdullah Mahdi, Romesh Kumar Salgotra, and Manmohan Sharma. "Screening microsatellite markers for establishing parental polymorphism in Indian rice (Oryza sativa L.)." Archives of Agriculture and Environmental Science 7, no. 4 (2022): 590–94. http://dx.doi.org/10.26832/24566632.2022.0704017.

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The experiment was conducted to investigate the parental diversity along the rice genome and to understand and screen out the SSR markers-indicated polymorphism between two indica rice (Oryza sativa L.) cultivars. Namely K343, the most well-liked rice variety in the hill zone of the Jammu Region, and RML22, a rice line created at IRRI, Philippines. The study is to select polymorphic markers (Simple Sequence Repeat- SSR) associated with hill ecologies rice cultivars and additional research projects like gene pyramiding and background selection to recover the recurrent parent genome (RPG) in bla

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G. Baldaniya, Vipul, Ajay V. Narwade, Pathik B. Patel, and Nilima Karmakar. "Molecular diversity analysis of rice (Oryza sativa L.) genotypes using RAPD and SSR marker." emergent Life Sciences Research 08, no. 02 (2022): 113–23. http://dx.doi.org/10.31783/elsr.2022.82113123.

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Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) molecular markers were used for detecting the genetic variability of 20 rice genotypes using 79 SSRs and 30 RAPD primers. Among primers used, a set of 16 SSR and 14 RAPD markers showed polymorphism, and banding patterns were scored as 1 (present) or 0 (absent) in the datasheet which was further analyzed by using Jaccard’s similarity coefficient and SAHN clustering. The number of alleles, PIC value, and heterozygosity for individual 16 SSR and 14 RAPD markers were used to assess the degree of polymorphism among the rice ge

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Duy, Le Quoc. "GENERAL CHARACTERISTICS OF CANISTEL (POUTERIA CAMPECHIANA) IN TRA VINH PROVINCE BASED ON MORPHOLOGICAL FEATURES." Journal of Southwest Jiaotong University 57, no. 4 (2022): 223–31. http://dx.doi.org/10.35741/issn.0258-2724.57.4.19.

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Canistel is a high-value fruit tree whose flesh is rich in nutrients and flavor. However, the research has not been focused on the management and conservation of the genetic resources of this crop. Molecular markers have been used in many studies to assess the diversity of different crops. However, studies evaluating the genetic diversity of canistel plants are still limited. The study aimed to evaluate the genetic diversity of canistel samples with morphological characteristics and molecular markers (inter-simple sequence repeat marker and random amplified polymorphic DNA marker). The diversi

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Bilous, Svitlana, Larysa Prysiazhniuk, Snizhana Chernii, Serghii Melnyk, Yurii Marchuk, and Artur Likhanov. "Genetic characterisation of centuries-old oak and linden trees using SSR markers." Folia Forestalia Polonica 64, no. 1 (2022): 58–68. http://dx.doi.org/10.2478/ffp-2022-0006.

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Abstract The main goal of this study was to identify the unique gene pool of old and historically valuable Quercus robur L. and Tilia cordata L. to be able to characterise their genetic diversity in order to determine the polymorphism by expressed sequence tag-single sequence repeat (EST-SSR) markers and identify the most valuable specimens. Morphological description, molecular genetic analysis, and statistical analysis were used in studies. The genetic distances between old-value trees of different Quercus L. and Tilia L. were determined based on EST-SSR markers and morphological characterist

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Davis, Clara L., Dawn Field, David Metzgar, et al. "Numerous Length Polymorphisms at Short Tandem Repeats in Human Cytomegalovirus." Journal of Virology 73, no. 8 (1999): 6265–70. http://dx.doi.org/10.1128/jvi.73.8.6265-6270.1999.

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ABSTRACT We show the presence of numerous short tandem repeats in the human cytomegalovirus (HCMV) genome and assess their usefulness as molecular markers. The genome is shown to contain at least 24 microsatellite regions that exhibit length polymorphisms. Insertion-deletion polymorphisms at these short tandem repeats are common (80% of repeats examined are polymorphic among two laboratory strains and 10 clinical isolates). This is the first report of widespread microsatellite length polymorphism in a viral genome. Some regions are highly polymorphic: one was revealed by DNA sequencing to cont

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Larekeng, Siti Halimah, Muh Restu, Gusmiaty Gusmiaty, and Rismawati Rismawati. "POLYMORPHISM OF SIMPLE SEQUENCE REPEAT REGIONS OF SULAWESI EBONY (DIOSPHYROS CELEBICA BAKH.) IN EXPERIMENTAL FOREST OF HASANUDDIN UNIVERSITY PROVENANCE." Agrotech Journal 1, no. 1 (2016): 37–44. http://dx.doi.org/10.31327/atj.v1i1.173.

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Polymerase Chain Reaction (PCR)-based molecular techniques have been used to detect the polymorphism in plants. The utilization of molecular markers plays essential role in germplasm characterization and plant breeding since the information of DNA marker technology can be exchanged between laboratories and should have standard method to be reproducible. The molecular aspect has been commonly linked to DNA isolation protocol and polymorphic molecular marker, thus can be used for molecular research recommendation purposes. The objectives of this study were to evaluate the capability of microsate

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Svitlana, Bilous, Prysiazhniuk Larysa, Chernii Snizhana, Melnyk Serghii, Marchuk Yurii, and Likhanov Artur. "Genetic characterisation of centuries-old oak and linden trees using SSR markers." FOLIA FORESTALIA POLONICA, SERIES A – FORESTRY 64, no. 1 (2022): 58–68. https://doi.org/10.2478/ffp-2022-0006.

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The main goal of this study was to identify the unique gene pool of old and historically valuable <em>Quercus robur </em>L. and Tilia cordata L. to be able to characterise their genetic diversity in order to determine the polymorphism by expressed sequence tag-single sequence repeat (EST-SSR) markers and identify the most valuable specimens. Morphological description, molecular genetic analysis, and statistical analysis were used in studies. The genetic distances between old-value trees of different <em>Quercus</em> L. and <em>Tilia</em> L. were determined based on EST-SSR markers and morpholo

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16

Cheon, Kyeong-Seong, Young-Min Jeong, Hyoja Oh, et al. "Development of 454 New Kompetitive Allele-Specific PCR (KASP) Markers for Temperate japonica Rice Varieties." Plants 9, no. 11 (2020): 1531. http://dx.doi.org/10.3390/plants9111531.

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Temperate japonica rice varieties exhibit wide variation in the phenotypes of several important agronomic traits, including disease resistance, pre-harvest sprouting resistance, plant architecture, and grain quality, indicating the presence of genes contributing to favorable agronomic traits. However, gene mapping and molecular breeding has been hampered as a result of the low genetic diversity among cultivars and scarcity of polymorphic DNA markers. Single nucleotide polymorphism (SNP)-based kompetitive allele-specific PCR (KASP) markers allow high-throughput genotyping for marker-assisted se

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Surduse, Bhavana, P. A. Mohanapure, V. C. Khelurkar, et al. "Molecular Characterization of chickpea genotypes and Identification of true hybrids by molecular markers." International Journal of Agricultural and Applied Sciences 2, no. 1 (2021): 41–49. http://dx.doi.org/10.52804/ijaas2021.214.

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Chickpea (Cicer arietinum L.) is the third most important grain legume cultivated in the arid and semi-arid regions of the world. In the present study Six crossing combinations were executed in chickpea comprising Chanoli and PKV Kabuli 4 as female parents and Virat, BDNGK-798 and WR- 315 as resistant male parents. Total 54 markers including 13 SCoT, 31 SSR, 5 STMS, 3 RAPD, 1 SCAR, and 1 ISSR, used for parental polymorphism and polymorphic markers UBC-855, 66 % for TA-59 and 100 % for TA-110, TA-135 and GA-16 were further used to hybridity assessments of F1 plants. The PIC value for polymorphi

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Caranta, Carole, Arnaud Thabuis, and Alain Palloix. "Development of a CAPS marker for the Pvr4 locus: A tool for pyramiding potyvirus resistance genes in pepper." Genome 42, no. 6 (1999): 1111–16. http://dx.doi.org/10.1139/g99-069.

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The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by the bulked segregant analysis method. Eight linked AFLP markers were mapped in an interval from 2.1 ± 0.8 to 13.8 ± 2.9 cM around this locus. The closest codominant AFLP marker was converted into a codomi

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Subramanian, V., S. Gurtu, R. C. Nageswara Rao, and S. N. Nigam. "Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay." Genome 43, no. 4 (2000): 656–60. http://dx.doi.org/10.1139/g00-034.

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Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic DNA (RAPD) assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yield

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Žiarovská, Jana, Katarína Ražná, Eloy C. Fernandéz, Danka Bošeľová, and Matúš Kyseľ. "Habitat-related specifity of iPBS fingerprint in European populations of Hedera helix L." Folia Oecologica 46, no. 1 (2019): 30–36. http://dx.doi.org/10.2478/foecol-2019-0005.

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AbstractInter primer binding site (iPBS) polymorphism was investigated for common ivy (Hedera helix, L.) to obtain the knowledge on genetic diversity in this species. Actually, a very limited information exists about application of DNA markers in Hedera helix. Natural and planted European populations of ivy were analysed using an iPBS marker 5′ACCTGGCGTGCCA3′ with a total number of 238 fragments generated. Of these, 86% were polymorphic. There were determined certain attributes of this marker such as the diversity index (DI) and polymorphism information content (PIC). The value of the diversit

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Vyas, Priyanka, Sunil K Jaiswal, Vinod Joshi, et al. "Analysis of Genetic Diversity among Populations of an Endangered Medicinal Plant Commiphora wightii (Arn.) Bhandari (Guggul) using Molecular Markers." Journal of Plant Science Research 39, no. 2 (2023): 141–52. http://dx.doi.org/10.32381/jpsr.2023.39.02.15.

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Commiphora wightii has massive economic value and a wide array of medicinal uses in both ancient and modern therapeutics. It has been observed that the number of C. wightii populations in Rajasthan are declining substantially because of anthropogenic activities in the form of over-exploitation for resin and fuel. Very limited molecular studies have been reported on the molecular characterization of Commiphora wightii at national and international level. The present investigation aims to assess the genetic diversity within and among different populations to determine the relationship of genetic

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Malysheva, I. E., L. V. Topchieva, E. L. Tikhonovich, I. V. Kurbatova, and O. V. Balan. "Association of FOXP3 gene -3279 C>A polymorphism with the risk of pulmonary sarcoidosis." Terapevticheskii arkhiv 89, no. 12 (2017): 64–67. http://dx.doi.org/10.17116/terarkh2017891264-67.

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Aim. To investigate the association of the polymorphic marker -3279 C>A of the FOXP3 gene with the risk of pulmonary sarcoidosis (PS) and to estimate the transcription level of this gene in the carriers of different genotypes of this polymorphic marker. Subjects and methods. The investigation included 99 patients of Russian ethnicity (mean age, 45.41±1.31 years) living in the Republic of Karelia, who were diagnosed with persistent PS, and 116 healthy donors (mean age, 42.06±1.30 years) in the control group. The alleles and genotypes of the polymorphic marker -3279 C>A of the FOXP3 gene w

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Saleh, Basel. "Phylogenetic Assessment Of Arthrocnemum Macrostachyum (Chenopodiaceae) Genotypes, Using Ramp Markers." Polish Botanical Journal 60, no. 2 (2015): 293–99. http://dx.doi.org/10.1515/pbj-2015-0022.

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AbstractRandom amplified microsatellite polymorphism (RAMP) marker technique was employed to test its usefulness for assessing phylogenetic relationships in three genotypes of Arthrocnemum macrostachyum (Moric.) Moris. & Delponte from Syria. PCR reactions with 21 RAMP primer combinations (PCs) distinguished 145 loci, 139 of which (95.862%) were polymorphic. The (AG)8TC/OPE18 primer combination generated the highest number of fragments (11 amplicons), and the (AC)8T/OPE04 primer combination the fewest (4 amplicons). Average estimated polymorphic information content (PIC) was 0.431, with an

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Nakkuntod, Maliwan, and Sunisa Luanglue. "Genetic Diversity of Nelumbo nucifera Gaertn. Cultivars Using DNA markers and Morphological traits." Chiang Mai Journal of Science 51, no. 6 (2024): 1–16. http://dx.doi.org/10.12982/cmjs.2024.087.

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Lotus (Nelumbo nucifera Gaertn.) is a species of aquatic plants in the Nelumbonaceae family. Currently, cultivation of ornamental flowers and breeding is widespread resulting in numerous hybrids and significant genetic diversity. Consequently, classification based on morphology becomes challenging and confusing. This research purposed to evaluate genetic diversity among nine samples in Phitsanulok province using sixteen qualitative and nine quantitative characters, along with three molecular markers: sequence-related amplified polymorphism (SRAP), random amplified polymorphic DNA (RAPD) and hi

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Gavrilova, T. V., A. R. Kinkulkina, A. S. Avagyan, and O. A. Svitich. "The investigation of the association of alleles and genotypes of the polymorphic marker T786C of the eNOS gene at various stages of primary open-angle glaucoma." Russian Ophthalmological Journal 17, no. 1 (2024): 28–31. http://dx.doi.org/10.21516/2072-0076-2024-17-1-28-31.

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Purpose: to study the association of the polymorphic marker T786C of the eNOS gene with various stages of primary open-angle glaucoma (POAG).Materials and methods. Peripheral blood of 90 patients aged 56 to 89 yrs (ave. 71 yrs) with POAG stages I, II, III was tested. DNA was isolated using a set of ribosorbents (Synthol company); subsequently, a real-time PCR reaction was performed on a DT-96 amplifier using the set to determine the polymorphic marker T786C in the eNOS gene.Results. For all groups of patients, the frequency of occurrence of alleles and genotypes was calculated, and the relatio

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Weir, B. J., R. G. St Pierre, and R. N. Chibbar. "RAPD Marker Polymorphism among Saskatoon Cultivars, Clones, and Seedlings." HortScience 32, no. 6 (1997): 1109–13. http://dx.doi.org/10.21273/hortsci.32.6.1109.

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Randomly amplified polymorphic DNA (RAPD) markers were used to distinguish among 16 cultivars of saskatoon (Amelanchier spp.). Eight 9-base, oligonucleotide primers amplified a total of 98 DNA fragments, of which 29 were useful as reproducible polymorphic markers. Twelve cultivars and two pairs of cultivars were uniquely characterized by these 29 markers. Polymorphism was not detected among five sources of the cv. Thiessen, whereas variability was found among seedlings from self-pollinated `Thiessen'. Samples of the cvs. Regent and Parkhill were indistinguishable from one of two sources, sugge

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Zhang, Yiping, and John R. Stommel. "049 Development of Molecular Markers Linked to High Pigment (hp) and Dark Green (dg) Loci in Tomato." HortScience 34, no. 3 (1999): 449D—449. http://dx.doi.org/10.21273/hortsci.34.3.449d.

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The carotenoids have an important influence on tomato fruit quality and enhance the fruit contribution to human nutrition. Expression of the high pigment (hp) locus in tomato results in increased total carotenoids and increased efficiency of utilization of the polyenes. A similar mutant, dark green (dg), contains higher level of chlorophyll in immature fruit and results in darker red pigmentation, both externally and internally in ripe fruit. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analyses were performed using two pairs of near isogenic lines

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Singh, Gurbir, Monika Yadav, Sibani Das, et al. "Marker Assisted Selection: A Novel Approach for Crop Improvement." International Journal of Plant & Soil Science 36, no. 12 (2024): 100–115. https://doi.org/10.9734/ijpss/2024/v36i125187.

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The process of marker-assisted selection, or marker-aided selection (MAS), selects a trait of interest indirectly by considering a marker linked to the trait (e.g., quality, productivity, disease resistance, and biotic stress tolerance) rather than the trait itself. This integration of marker data with traditional selection approaches has become a widely studied and recommended method for advancing breeding programs. This technique has been extensively researched and recommended for animal and plant breeding. Here, we combine marker data with conventional selection to choose the best candidate

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Jahnke, Gizella, József Smidla, and Peter Poczai. "MolMarker: A Simple Tool for DNA Fingerprinting Studies and Polymorphic Information Content Calculation." Diversity 14, no. 6 (2022): 497. http://dx.doi.org/10.3390/d14060497.

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Molecular markers and mapping are used to analyze an organism’s genes. They allow the selection of target genetic areas based on marker genotype (and not trait phenotype), facilitate the study of genetic variability and diversity, create linkage maps, and follow individuals or lines carrying certain genes. They may be used to select parental genotypes, remove linkage drag in back-crossing, and choose difficult-to-measure characteristics. Due to a lack of genetic variety in crops, the gene pools of wild crop relatives for future agricultural production have been examined. The invention of RFLP

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Woeste, Keith, Gale H. McGranahan, and Robert Bernatzky. "Randomly Amplified Polymorphic DNA Loci from a Walnut Backcross [(Juglans hindsii × J. regia) × J. regia]." Journal of the American Society for Horticultural Science 121, no. 3 (1996): 358–61. http://dx.doi.org/10.21273/jashs.121.3.358.

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Twenty-five random decamer primers were used to evaluate the level of polymorphism between Persian walnut and the Northern California black walnut. Sixty-six randomly amplified polymorphic DNA (RAPD) markers were identified using an interspecific walnut backcross population [(Juglans hindsii × J. regia) × J. regia]. Segregation data from these polymorphisms were joined to a previously published set of restriction fragment-length polymorphism (RFLP) marker data to expand the genetic map of walnut to 107 markers in 15 linkage groups.

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Simic, Domagoj, Tatjana Ledencan, Antun Jambrovic, et al. "SNP and SSR marker analysis and mapping of a maize population." Genetika 41, no. 3 (2009): 237–46. http://dx.doi.org/10.2298/gensr0903237s.

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Although highly polymorphic SSRs are currently the marker of choice worldwide in maize breeding, single nucleotide polymorphisms (SNPs) as a newer marker system are recently used more extensively. The objective of this study was investigate the utility of SSR and SNP markers for mapping of a maize population adapted to conditions of Southeast Europe. Total of 294 F2:3 lines derived from a biparental mapping population were genotyped using 121 polymorphic SNP and SSR markers. The SNP markers were analyzed using the SNPlex technology. 56 of the 142 tested SNPs (39%) were polymorphic between the

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Nagl, Nevena, Ksenija Taski-Ajdukovic, Andrea Popovic, Zivko Curcic, Dario Danojevic, and Lazar Kovacev. "Estimation of genetic variation among related sugar beet genotypes by using RAPD." Genetika 43, no. 3 (2011): 575–82. http://dx.doi.org/10.2298/gensr1103575n.

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In marker assisted breeding programs, determination of genome polymorphism and development of suitable molecular markers is of the greatest importance. The aim of this research was development of RAPD markers, which will enable quick and cost efficient DNA polymorphism analysis among closely related sugar beet genotypes. The research was conducted on twelve sugar beet genotypes from population of closely related genotypes. Reactions with eight RAPD primers and five primer mixtures resulted in stable and reproducible bands in all samples, with 44 polymorphic and 14 monomorphic loci, and average

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Guasmi, Ferdaous, Walid Elfalleh, Hédia Hannachi, et al. "The Use of ISSR and RAPD Markers for Genetic Diversity among South Tunisian Barley." ISRN Agronomy 2012 (January 3, 2012): 1–10. http://dx.doi.org/10.5402/2012/952196.

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Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) were assayed to determine the genetic diversity of 80 barley specimens from South Tunisia. The ISSR primers showed variation in the percentage of polymorphism, band informativeness (Ib), and resolving power (Rp). The percentage of polymorphism is 66.67%, the average Ib ranged from 0.24 to 0.39, while Rp ranged from 0.74 to 1.16. In RAPD analysis, three primers yielded a total of 17 scorable bands, which are all polymorphic. The three polymorphic primers exhibited variation with regard to average band informativeness

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Zhang, Xinyi, Li Liao, Zhiyong Wang, Changjun Bai, and Jianxiu Liu. "Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers." HortScience 51, no. 8 (2016): 972–79. http://dx.doi.org/10.21273/hortsci.51.8.972.

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Molecular genetic diversity and relationships among 86 Chrysopogon aciculatus (Retz.) Trin. accessions were assessed using intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Twenty-five ISSR markers generated 283 amplification bands, of which 266 were polymorphic. In addition, 576 polymorphic bands were detected from 627 bands amplified using 30 SRAP primers. Both marker types revealed a high level of genetic diversity, with ISSR markers showing a higher proportion of polymorphic loci (PPL; 94%) than SRAP markers (91.87%). The ISSR and SRAP data were

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Rushdi, H. E., R. E. A. Moghaieb, H. Abdel-Shafy, and M. A. M. Ibrahim. "Association between microsatellite markers and milk production traits in Egyptian buffaloes." Czech Journal of Animal Science 62, No. 9 (2017): 384–91. http://dx.doi.org/10.17221/80/2016-cjas.

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The objectives were to evaluate polymorphism in ten microsatellite markers and to demonstrate their association with milk production traits in the Egyptian buffaloes, based on the cross-species transferability of microsatellites from cattle to buffalo. A total of 17 439 daily milk records from the first five lactations were subjected to analyses, in which records from 5 to 290 days in milk were only included. The analysis revealed that eight out of the ten bovine markers analyzed were polymorphic. The means of the number of alleles, effective number of alleles, and fixation index within marker

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Jiang, Shuang, Haishan An, Xiaoqing Wang, Chunhui Shi, Jun Luo, and Yuanwen Teng. "The Genotypes of Polymorphic Simple Sequence Repeat Loci Revealed by Whole-genome Resequencing Data of 30 Pyrus Accessions." Journal of the American Society for Horticultural Science 144, no. 5 (2019): 321–28. http://dx.doi.org/10.21273/jashs04713-19.

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Simple sequence repeats (SSRs) are widely used in cultivar identification, genetic relationship analysis, and quantitative trait locus mapping. Currently, the selection of hybrid progeny plants in molecular marker-assisted breeding mostly relies on SSR markers because of their ease of operation. In Pyrus, a large number of SSR markers have been developed previously. The method to identify polymorphic SSRs quickly is still lacking in cultivated as well as wild pear species. We present a large number of polymorphic SSRs identified using a quick in silico approach applied across 30 cultivated and

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Dossett, Michael, Jill M. Bushakra, Barbara Gilmore, et al. "Development and Transferability of Black and Red Raspberry Microsatellite Markers from Short-Read Sequences." Journal of the American Society for Horticultural Science 140, no. 3 (2015): 243–52. http://dx.doi.org/10.21273/jashs.140.3.243.

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The advent of next-generation, or massively parallel sequencing technologies has been a boon to the cost-effective development of molecular markers, particularly in nonmodel species. Here, we demonstrate the efficiency of microsatellite or simple sequence repeat (SSR) marker development from short-read sequences in black and red raspberry (Rubus occidentalis L. and R. idaeus L., respectively), compare transferability of markers across species, and test whether the rate of polymorphism in the recovered markers can be improved upon by how marker sequences are chosen. From 28,536,412 black raspbe

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Mariutsa, А., N. Borysenko, V. Gushchin, and I. Grytsynyak. "Use of ISSR markers for genotyping an experimental group of largemouth bass Micropterus salmoides (LACEPEDE, 1802), reared in ponds of Polissia of Ukraine." Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva, no. 1 (186) (May 24, 2024): 145–50. http://dx.doi.org/10.33245/2310-9289-2024-186-1-145-150.

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The aim of this study was to investigate the genetic structure of the experimental group of largemouth bass reared in the ponds of Polissia of Ukraine using ISSR markers. To accomplish these tasks, ISSR genotyping of the genetic structure of largemouth bass was performed using four fragments of trinucleotide loci. The genetic structure of the experimental group of largemouth bass of the pond fsh farm "Nyvka" was characterised using 4 primers B – (GAG)6C; C – (AGC)6G; D – (ACC)6G and E – (AGC)6C. Fins fragments were used for the study. In the course of the work, the optimal conditions for ISSR-

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Anggraheni, Yuliana Galih Dyan, Enung Sri Mulyaningsih, Dody Priadi, et al. "Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice." Jurnal Biodjati 5, no. 1 (2020): 50–62. http://dx.doi.org/10.15575/biodjati.v5i1.7990.

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SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic marke

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Çakır, Esra. "Evaluation of Genetic Diversity in Wild Emmer Wheat (Triticum dicoccoides) and Durum Wheat (Triticum durum) Accessions Using CAAT and SCoT Markers." Agronomy 15, no. 2 (2025): 284. https://doi.org/10.3390/agronomy15020284.

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Wild emmer is one of the progenitors of wheat, with a high genetic potential for breeding. Continuous evaluations of emmer and other progenitor species are necessary for long-term improvement in yield, agronomic, and stress-related traits. For this purpose, genetic diversity and relationships among 43 wild emmer (Triticum dicoccoides) and 5 durum wheat (Triticum durum) accessions were determined using two DNA marker systems, CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) markers. CAAT and SCoT markers generated 63 and 76 polymorphic bands, averaging 9 and 7.6 bands per pr

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Tanhuanpää, Pirjo, Ruslan Kalendar, Jaana Laurila, Alan H. Schulman, Outi Manninen, and Elina Kiviharju. "Generation of SNP markers for short straw in oat (Avena sativa L.)." Genome 49, no. 3 (2006): 282–87. http://dx.doi.org/10.1139/g05-100.

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Short straw is a desired trait in oat germplasm (Avena sativa L.). Marker-assisted selection, a key tool for achieving this objective, is limited by the presence and number of available markers. Here, we have attempted to develop markers sufficiently linked to a gene specifying short straw so that marker-assisted selection could be applied. Bulked-segregant analysis was used to identify anonymous PCR-based markers associated with the dwarfing gene Dw6 in an F2 population from the cross between A. sativa 'Aslak' and A. sativa 'Kontant'. One random amplified polymorphic DNA (RAPD) and 1 retrotra

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Wen, Sulin, Hong Zhao, Manying Zhang, Guang Qiao, and Xiaohui Shen. "IRAPs in Combination with Highly Informative ISSRs Confer Effective Potentials for Genetic Diversity and Fidelity Assessment in Rhododendron." International Journal of Molecular Sciences 24, no. 8 (2023): 6902. http://dx.doi.org/10.3390/ijms24086902.

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The species belonging to the Rhododendron genus are well-known for their colorful corolla. Molecular marker systems have the potential to elucidate genetic diversity as well as to assess genetic fidelity in rhododendrons. In the present study, the reverse transcription domains of long terminal repeat retrotransposons were cloned from rhododendrons and used to develop an inter-retrotransposon amplified polymorphism (IRAP) marker system. Subsequently, 198 polymorphic loci were generated from the IRAP and inter-simple sequence repeat (ISSR) markers, of which 119 were derived from the IRAP markers

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Sebko, Tat'yana Vasil'evna, Yu E. Dobrokhotova, T. A. Ivanova, et al. "Genetic markers for insulin resistance in gestational diabetes mellitus." Russian Medicine 17, no. 1 (2011): 19–23. http://dx.doi.org/10.17816/rmj37883.

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KCNJ, TCF7L2, PPARG2, ADIPOQ, ADIPOR, and ADIPOR2 gene polymorphisms and hormonal and metabolic markers (immunoreactive insulin, proinsulin, C-peptide, glycemia, lipid spectrum, leptin, glycated hemoglobin) were determined in 185 patients with gestational diabetes mellitus (GDM); of them 80 patients were diagnosed as having GDM during current pregnancy; GDM was diagnosed in 105 patients 4-19 years (14.6±3.8 years) ago. Analysis of the distribution of the frequencies of alleles and genotypes of polymorphic markers in the study genes in healthy versus GDM pregnant women showed a significant incr

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Lee, Tong Geon, Reza Shekasteband, Naama Menda, Lukas A. Mueller, and Samuel F. Hutton. "Molecular Markers to Select for the j-2–mediated Jointless Pedicel in Tomato." HortScience 53, no. 2 (2018): 153–58. http://dx.doi.org/10.21273/hortsci12628-17.

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The jointless pedicel trait of tomato conferred by the j-2 gene is widely used in processing markets for stem-free removal of fruit to accommodate mechanized harvest. Although current utilization of j-2 for fresh-market tomato breeding is limited, interest in this trait may increase as breeders seek to address high labor costs through the development of mechanically harvestable cultivars for the fresh market. Yet, the introduction of this trait into new market classes heavily relies on phenotypic selection because there are presently no high-throughput methods available to genotype j-2. Reliab

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Singh, Vivek Kumar, Brahma Deo Singh, Amit Kumar, et al. "Marker-Assisted Introgression of Saltol QTL Enhances Seedling Stage Salt Tolerance in the Rice Variety “Pusa Basmati 1”." International Journal of Genomics 2018 (2018): 1–12. http://dx.doi.org/10.1155/2018/8319879.

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Marker-assisted selection is an unequivocal translational research tool for crop improvement in the genomics era. Pusa Basmati 1 (PB1) is an elite Indian Basmati rice cultivar sensitive to salinity. Here, we report enhanced seedling stage salt tolerance in improved PB1 genotypes developed through marker-assisted transfer of a major QTL, Saltol. A highly salt tolerant line, FL478, was used as the Saltol donor. Parental polymorphism survey using 456 microsatellite (SSR)/QTL-linked markers revealed 14.3% polymorphism between PB1 and FL478. Foreground selection was carried out using three Saltol-l

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Zavarykina, T. M., P. K. Lomskova, M. A. Kapralova, et al. "Association of polymorphic markers of the <i>XRCC1</i>, <i>ERCC5</i>, <i>TP53</i>, <i>CDKN1A1</i> genes with the survival of patients after platinum-based chemotherapy for triple negative breast cancer." Tumors of female reproductive system 18, no. 4 (2023): 69–80. http://dx.doi.org/10.17650/1994-4098-2022-18-4-69-80.

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Background. Breast cancer is the most common cancer among women. Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, in which there are no special targets for therapy. Therefore chemotherapy is still leading treatment for TNBC including the regiments with platinum drugs.Aim. To study the association of polymorphic markers of the genes XRCC1 (rs25487), ERCC5 (rs17655), TP53 (rs1042522), CDKN1A1 (rs1801270) with progression-free survival (PFS) and overall survival (OS) of TNBC patients after platinum-based neoadjuvant chemotherapy.Materials and methods. Polymorp

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Demirel, Ufuk, İlknur Tındaş, Caner Yavuz, Faheem Shehzad Baloch, and Mehmet Emin Çalışkan. "Assessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system." Plant Genetic Resources: Characterization and Utilization 16, no. 2 (2017): 137–45. http://dx.doi.org/10.1017/s1479262117000041.

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AbstractHaving knowledge on genetic similarity and DNA profile of potato genotypes facilitates a breeder's decision for parent selection and provides accurate variety identification. Fingerprinting and identification of genetic similarity among 151 potato genotypes were achieved using an inter-primer-binding sites (iPBS) retrotransposon marker system. Our study is the first application of iPBS markers for fingerprinting and distinguishing large numbers of Solanum tuberosum genotypes. Initially, 16 potato genotypes were screened using 45 iPBS retrotransposon markers to identify polymorphisms. S

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Saikrishna, Madastu, C. L. Maurya, C. N. Neeraja, et al. "Simple Sequence Repeat (SSR) Marker-Based Parental Polymorphism Analysis between Improved Samba Mahsuri and Karuppunel for Grain Iron and Zinc Content in Rice (Oryza sativa L.)." Journal of Advances in Biology & Biotechnology 28, no. 5 (2025): 737–51. https://doi.org/10.9734/jabb/2025/v28i52335.

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Aim: The present study aimed to assess parental polymorphism between two genetically contrasting rice genotypes using microsatellite (SSR) markers, with a focus on identifying markers associated with grain micronutrient traits, particularly iron and zinc content. Place and Duration of Study: The study was conducted at the Biotechnology Section, ICAR–Indian Institute of Rice Research (IIRR), Rajendranagar, Hyderabad, Telangana, India, during the period from August 2023 to September 2024. Methodology: Genomic DNA was extracted from young leaf tissues of two contrasting parents, Improved Samba Ma

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Ferguson, Moira M., and Roy G. Danzmann. "Role of genetic markers in fisheries and aquaculture: useful tools or stamp collecting?" Canadian Journal of Fisheries and Aquatic Sciences 55, no. 7 (1998): 1553–63. http://dx.doi.org/10.1139/f98-096.

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We comment on the role of genetic markers in fisheries and aquaculture with a view to the future. Our goal is to encourage researchers to evaluate the molecular markers they need to deploy and shift their thinking away from analyses of stock structure towards more aggressive pursuit of questions related to genome structure and function. Examples illustrate that no one marker type is appropriate for all applications. Choice should be based on the evolutionary genetic attributes of both the species and the marker loci themselves. We evaluate three relatively new marker types: mitochondrial DNA (

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Dalbó, M. A., G. N. Ye, N. F. Weeden, W. F. Wilcox, and B. I. Reisch. "Marker-assisted Selection for Powdery Mildew Resistance in Grapes." Journal of the American Society for Horticultural Science 126, no. 1 (2001): 83–89. http://dx.doi.org/10.21273/jashs.126.1.83.

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The efficiency of marker-assisted selection for powdery mildew (Uncinula necator (Schw.) Burr) resistance in grapes (Vitis L. sp.) was studied using molecular markers associated with a major QTL (quantitative trait loci) for this trait. Initially, genetic maps were constructed from a segregating population of the cross `Horizon' × Illinois 547-1 (a hybrid between V. rupestris Scheele and V. cinerea Engelm.). A major QTL from Ill. 547-1, the resistant parent, explained 41% of the variation. One RAPD (randomly amplified polymorphic DNA) marker and one AFLP (amplified fragment length polymorphism

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