Academic literature on the topic 'Polyspermy'

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Journal articles on the topic "Polyspermy"

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Iwao, Yasuhiro. "Egg activation in physiological polyspermy." REPRODUCTION 144, no. 1 (2012): 11–22. http://dx.doi.org/10.1530/rep-12-0104.

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Fertilization is indispensable not only for restoring diploid genomes but also for the initiation of early embryonic cell cycles in sexual reproduction. While most animals exhibit monospermy, which is ensured by polyspermy blocks to prevent the entry of extra sperm into the egg at fertilization, several animals exhibit physiological polyspermy, in which the entry of several sperm is permitted but only one sperm nucleus participates in the formation of a zygote nucleus. Polyspermy requires that the sperm transmit the egg activation signal more slowly, thus allowing the egg to accept several spe
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Funahashi, Hiroaki. "Polyspermic penetration in porcine IVM - IVF systems." Reproduction, Fertility and Development 15, no. 3 (2003): 167. http://dx.doi.org/10.1071/rd02076.

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Although techniques for in vitro production of porcine embryos have proceeded very rapidly during the past decade, polyspermic penetration still remains a persistent obstacle to porcine in vitro fertilization (IVF) systems. Considerable research on in vitro polyspermic penetration in porcine in vitro-matured (IVM) oocytes has been undertaken to try to solve this problem. In the current paper, recent advancements in overcoming the problems of polyspermy in porcine IVF systems are reviewed. Partial induction of the acrosome reaction of boar spermatozoa in IVF media that contain caffeine is likel
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Oterino, J., G. Sánchez Toranzo, L. Zelarayán, J. N. Valz-Gianinet, and M. I. Bühler. "Cortical granule exocytosis in Bufo arenarum oocytes matured in vitro." Zygote 9, no. 3 (2001): 251–59. http://dx.doi.org/10.1017/s0967199401001277.

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Denuded Bufo arenarum oocytes matured in vitro by progesterone treatment exhibited abnormal segmentation due to the penetration of more than one sperm. These oocytes were able to respond to activation stimuli and exhibited the external signs characteristic of activation. However, the prevention of polyspermy was not effective in these oocytes, which exhibited numerous sperm in their cytoplasm. The aim of this work was to analyse the cortical reaction in polyspermic Bufo arenarum oocytes matured in vitro. The result indicate that the cortical reaction of these oocytes seems to occur with a chro
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Galli, C., G. Crotti, P. Turini, I. Lagutina, and G. Lazzari. "295 INFLUENCE OF FERTILIZATION MEDIUM ON THE INCIDENCE OF POLYSPERMY IN THE PIG." Reproduction, Fertility and Development 19, no. 1 (2007): 263. http://dx.doi.org/10.1071/rdv19n1ab295.

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The in vitro production of embryos is well established in most domestic species including cattle, pigs, sheep, and goats. However, a major problem of IVF in the pig is the high incidence of polyspermy. In our laboratory, we investigated the effect of 2 different media, TALP and SOFaa, on the rate of fertilization and polyspermy of pig oocytes. Preliminary experiments indicated that TALP provided the highest fertilization but also the highest polyspermy rates, as reported in the literature (Coy et al. 2002 Reproduction 124, 279–288). By contrast, much lower polyspermy rates but also much lower
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Schmidt, K., A. Clark, A. Mello, et al. "The effects of glucuronic acid and N-acetyl-D-glucosamine on in vitro fertilisation of porcine oocytes." Reproduction, Fertility and Development 28, no. 8 (2016): 1223. http://dx.doi.org/10.1071/rd14226.

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High incidences of polyspermic penetration continue to challenge researchers during porcine in vitro fertilisation (IVF). The aim of this study was to reduce the incidence of polyspermy by increasing the perivitelline space thickness with glucuronic acid and N-acetyl-D-glucosamine (GlcNAc) supplementation during oocyte maturation. After maturation, zona pellucida and perivitelline space thicknesses, intracellular glutathione concentrations and fertilisation kinetics were measured, in addition to embryonic cleavage and blastocyst formation at 48 h and 144 h after IVF, respectively. There were n
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Gonçalves, Paulo Bayard, and Charles Graves. "Fetuin: a tool to study the block to polyspermy." Ciência Rural 27, no. 1 (1997): 127–31. http://dx.doi.org/10.1590/s0103-84781997000100022.

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The effect of fetuin, an a1-glycoprotein that has protease inhibitor activity, on the biock to potyspermy was determined. Cumulus-oocyte complexes from eCG primed mice were matured in vitro in the presence of 0, 0.01, 0.1, l and 10mg/ml of fetuin in modifica TCM 199. Both in vivo and in vitro matured oocytes were fertilized in the presence of fetuin and incubated for 6 and 24h. Fetuin present in a concentration of 1 mg/ml in the ferlilization but not in the maturation medium was able to induce polyspermy in 52.4% of the eggs. There was a positive relationship between concentration of fetuin in
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Snook, Rhonda R., David J. Hosken, and Timothy L. Karr. "The biology and evolution of polyspermy: insights from cellular and functional studies of sperm and centrosomal behavior in the fertilized egg." REPRODUCTION 142, no. 6 (2011): 779–92. http://dx.doi.org/10.1530/rep-11-0255.

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Recent studies of centrosome biogenesis, microtubule dynamics, and their management point to their role in mediating conditions such as aging and cancer. Centrosome dysfunction is also a hallmark of pathological polyspermy. Polyspermy occurs when the oocyte is penetrated by more than one sperm and can be pathological because an excess of centrosomes compromises development. However, in some taxa, multiple sperm enter the egg with no apparent adverse effect on zygote viability. Thus, some taxa can manage excess centrosomes and represent cases of non-pathological polyspermy. While these two form
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Park, C. H., B. S. Koo, J. I. Yun, et al. "281REDUCTION OF POLYSPERMY IN PORCINE IN VITRO FERTILIZATION BY MODIFIED SWIM-UP METHOD." Reproduction, Fertility and Development 16, no. 2 (2004): 260. http://dx.doi.org/10.1071/rdv16n1ab281.

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In vitro production (IVP) of porcine embryos facilitates research related to biotechnology and biomedicine. Even though many attempts have been made to optimize the IVP of porcine embryos, the outcome is still unsatisfactory compared to other species, such as mouse and cattle. The high incidence of polyspermic fertilization is one of the major causes lowering the overall efficiency of porcine IVF. The common procedure for fertilization in vitro involves the co-culture of both gametes in the medium drop, which increases sperm concentration and incidence of polyspermy. Therefore, the present stu
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Oterino, J., G. Sánchez Toranzo, L. Zelarayán, and M. I. Bühler. "Polyspermy in Bufo arenarum oocytes matured in vitro." Zygote 5, no. 3 (1997): 267–71. http://dx.doi.org/10.1017/s0967199400003713.

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SummaryFull-grown ovarian oocytes of the amphibian Bufo arenarum were induced to mature in vitro by removing the follicular layers (spontaneous maturation) or by treatment with progesterone (hormone-induced maturation). These oocytes were then treated with trypsin and inseminated with homologous spermatozoa. Oocytes matured in vivo that had not undergone any influence of the oviducts (coelomic oocytes), inseminated under the same experimental conditions, were used as controls. The results show that oocytes induced to mature in vitro and exhibiting apparently normal signs of activation were pol
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WEBSTER, BOBBY W., ANNE COLSTON WENTZ, KEVIN G. OSTEEN, B. JANE ROGERS, and WILLIAM K. VAUGHN. "Hormonal Correlates with Polyspermy." Annals of the New York Academy of Sciences 442, no. 1 In Vitro Fert (1985): 332–35. http://dx.doi.org/10.1111/j.1749-6632.1985.tb37537.x.

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Dissertations / Theses on the topic "Polyspermy"

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Wong, Julian Ling-Chao. "Molecular mechanisms of the animal block to polyspermy /." View online version; access limited to Brown University users, 2005. http://wwwlib.umi.com/dissertations/fullcit/3174518.

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Schmidt, Victor T. "The effects of polyspermy, sexual conflict, and gene introgression on gamete incompatibility." View electronic thesis (PDF), 2009. http://dl.uncw.edu/etd/2009-2/schmidtv/victorschmidt.pdf.

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Grullón, Yunén Luis Alberto. "Función del sistema plasminógeno-plasmina en la fecundación de ovocitos bovinos y porcinos." Doctoral thesis, Universidad de Murcia, 2010. http://hdl.handle.net/10803/32113.

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El objetivo de este trabajo consistió en describir el papel del sistema plasminógeno/plasmina (PLG/PLA) en la fecundación bovina y porcina. Mediante fecundación in vitro, demostramos que la presencia de PLG ó PLA en el medio de coincubación de los gametos disminuía la penetración de los espermatozoides en los ovocitos y su unión a la zona pelúcida (ZP). Esta disminución no se debía a alteraciones de la funcionalidad espermática ni a cambios en la resistencia de la ZP a la proteolisis, sino a que la PLA provocaba la liberación de los espermatozoides adheridos a la ZP. Mediante inmunofluorescenc
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Coy, Fuster Pilar. "Fecundación "in vitro" en la especie porcina: influencia de diferentes condiciones de cocultivo." Doctoral thesis, Universidad de Murcia, 1991. http://hdl.handle.net/10803/10858.

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Con el presente trabajo se ha pretendido investigar la influencia de diversos factores relacionados con el cocultivo de los gametos porcinos, sobre los resultados de la fecundación" in vitro" (AV), fundamentalmente con la intención de mejorar la eficacia actual del sistema de AV en cuanto a la consecución de embriones viables (fecundaciones monospérmicas). Para ello, se han utilizado 129 hembras porcinas prepúberes a las que se indujo la ovulación mediante un tratamiento con 1250 U.I. de PMSG y 750 U.I. de HCG. El tratameinto empleado resultó eficaz para los fines perseguidos en un 80'62% de l
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Cánovas, Bernabé Sebastián. "Interacciones homólogas y heterólogas in vitro de gametos porcinos, bovinos y humanos y sus aplicaciones en el estudio de la fecundación." Doctoral thesis, Universidad de Murcia, 2007. http://hdl.handle.net/10803/10851.

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La interacción entre gametos es crucial para la fecundación. La zona pelúcida (ZP) se considera responsable de bloquear la polispermia, pero in vitro estas funciones no son totalmente eficientes. La polispermia es frecuente en fecundación in vitro (FIV) en porcino y bovino, mientras que la interacción heteróloga espermatozoide-ovocito ha sido demostrada. Los objetivos fueron estudiar el bloqueo de la polispermia para mejorar los resultados de FIV e investigar las interacciones heterólogas entre espermatozoide humano y ovocito porcino. Los resultados demuestran que se produce endurecimiento d
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Gil, Corbalán María Antonia. "Influencia de diferentes condiciones de cocultivo sobre la fecundación y la producción in vitro de embriones porcinos." Doctoral thesis, Universidad de Murcia, 2001. http://hdl.handle.net/10803/10979.

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Para reducir la incidencia de las penetraciones polispérmicas en los sistemas de fecundación in vitro porcina (FIV), los objetivos del presente trabajo fueron estudiar: 1) el efecto de tres volúmenes de medio de coincubación (2, 1 y 0'1 ml) y tres números de ovocitos (50, 30 y 15), inseminados con 6 x 105 espermatozoides /ml (primera experiencia) y 2000:1 espermatozoides:ovocito (segunda experiencia); 2) el efecto de la presencia de células del cumulus durante la FIV de ovocitos inseminados con diferentes ratios espermatozoides:ovocito (2000:1; 3000:1; 4000:1, 6000:1 y 8000:1), en 0'1 ml de me
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Francis, Matthew D. "Interspecific Competition Between Hygrophila polysperma and Ludwigia repens, Two Species of Importance in the Comal River, Texas." Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc278133/.

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Hygrophila polysperma is a plant native to Asia that has been introduced into the Comal River, TX and is thriving while Ludwigia repens, a species native to the river appears to be declining. Both plants have similar morphologies and occupy similar habitats in the river. Two plant competition experiments were conducted to examine the competitive interactions between the two species. First, an experimental design was developed in which established Ludwigia plants were 'invaded' by sprigs of Hygrophila to determine if established Ludwigia populations would be negatively impacted by invasion. The
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Wang, Shun-Chang, and 王舜璋. "The Purification and Characterization of Cystatin and Its Role in Preventing Polyspermy during Fertilization in Carp(Cyprinus carpio)." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/43999463299425414376.

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博士<br>國立臺灣大學<br>生化科學研究所<br>90<br>A sperm-agglutinating factor was purified from carp eggs and the conditioned medium of cortical reacted eggs (CM). It was identified to be the carp ovarian cystatin. Three cystatin isoforms were found. The cystatin isolated from the CM had a higher sperm-agglutinating activity than that isolated from eggs although they have identical N-terminal amino acid sequence, mass, and positive charges. Differences in sperm-agglutinating activity between the cystatins of the CM and eggs may be caused by the different conformations because they differ in circular dichroism
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IEGOROVA, Viktoriia Barativna. "Polyspermie u jeseterů dává vzniknout životaschopným mozaikám." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-390127.

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Chapter 1 (General Introduction), Chapter 2 (Polyspermy produces viable haploid/diploid mosaics in sturgeon), Chapter 3 (First evidence of viable progeny from three interspecific parents in sturgeon), Chapter 4 (General discussion, English Summary, Czech Summary, Acknowledgements, List of Publications, Training and Supervision Plan during Study, Curriculum Vitae). The thesis is written in English, contains 71 pages. The results were published in two scientific journals. The actuality of work: The majority of sturgeons are critically endangered and at the same time they are producers of the mos
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Book chapters on the topic "Polyspermy"

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Brawley, Susan H. "Polyspermy Blocks in Fucoid Algae and the Occurrence of Polyspermy in Nature." In Mechanism of Fertilization: Plants to Humans. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-83965-8_28.

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Brawley, Susan H. "The polyspermy block in fucoid algae." In Cell Walls and Surfaces, Reproduction, Photosynthesis. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-48652-4_10.

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Longo, Frank J. "Blocks to polyspermy and the cortical granule reaction." In Fertilization. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4899-3264-8_6.

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Santella, L. "The Cytoskeleton and Polyspermy in Sea Urchin Eggs." In Mechanism of Fertilization: Plants to Humans. Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-83965-8_47.

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Iwao, Yasuhiro. "Egg Activation in Polyspermy: Its Molecular Mechanisms and Evolution in Vertebrates." In Sexual Reproduction in Animals and Plants. Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54589-7_15.

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Dean, Jurrien. "A ZP2 Cleavage Model of Gamete Recognition and the Postfertilization Block to Polyspermy." In Sexual Reproduction in Animals and Plants. Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54589-7_33.

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Iwao, Yasuhiro, and Kenta Izaki. "Universality and Diversity of a Fast, Electrical Block to Polyspermy During Fertilization in Animals." In Diversity and Commonality in Animals. Springer Japan, 2018. http://dx.doi.org/10.1007/978-4-431-56609-0_24.

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Eisenhut, Markus, and Thomas G. Honegger. "Identification of Phallusia mammillata Egg β-N-Acetylhexosaminidase with a Potential Role in Prevention of Polyspermy." In The Biology of Ascidians. Springer Japan, 2001. http://dx.doi.org/10.1007/978-4-431-66982-1_16.

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Mizushima, Shusei. "Fertilization 2: Polyspermic Fertilization." In Advances in Experimental Medicine and Biology. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3975-1_7.

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Dale, Brian. "Polyspermy." In Encyclopedia of Reproduction. Elsevier, 2018. http://dx.doi.org/10.1016/b978-0-12-801238-3.64472-0.

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