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1

Lynch, Daniel E., Lisa C. Thomas, Graham Smith, Karl A. Byriel, and Colin H. L. Kennard. "A New Supramolecular Synthon Using N-Methylaniline. The Crystal Structure of the 1 : 1 Adduct of N-Methylaniline with 5-Nitrofuran-2-carboxylic Acid." Australian Journal of Chemistry 51, no. 9 (1998): 867. http://dx.doi.org/10.1071/c98072.

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The crystal structure of the 1 : 1 adduct of N-methylaniline with 5-nitrofuran-2-carboxylic acid has been determined by single-crystal X-ray diffraction. Crystals are monoclinic, space group P21/c with Z 4 in a cell of dimensions a 8·467(5), b 6·106(2), c 23·95(1) Å, β 94·48(3)°. The molecules associate in a tetrameric, proton-transfer formation which has potential as a new supramolecular synthon.
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2

Ichinose, Yukito, Kaoru Kubota, Giorgio Scagliotti, et al. "Phase III study (MONET1) of motesanib plus carboplatin/paclitaxel (C/P) in patients with advanced nonsquamous non-small cell lung cancer (NSCLC): Asian subgroup analysis." Journal of Clinical Oncology 30, no. 15_suppl (2012): 7549. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.7549.

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7549 Background: MONET1 evaluated overall survival (OS) in patients (pts) with nonsquamous NSCLC receiving motesanib (an oral VEGFR 1, 2 and 3, PDGFR and Kit inhibitor) plus C/P compared with pts receiving placebo plus C/P. Analysis of the total population (N=1090) showed that motesanib + C/P did not significantly improve OS vs C/P alone (primary endpoint). Here we present results of a subgroup analysis of Asian pts. Methods: Asian pts (Japan, S. Korea, Philippines, Hong Kong, Taiwan, Singapore) with stage IIIB/IV or recurrent nonsquamous NSCLC and no prior systemic therapy for advanced NSCLC
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3

Choua, O. "Plaies pénétrantes de l’abdomen en pratique civile : notre attitude à N’djaména." Journal Africain de Chirurgie 1, no. 3 (2012): 155–60. http://dx.doi.org/10.61585/pud-jafrchir-v1n304.

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The management of penetrating abdominal wounds is always debated, especially with regard to the stable and asymptomatic patients. Objectives: To analyze the results of the various behaviors suggested by the literature and adopted in our context, and evaluate the feasibility of the observation known as “selective conservatism” for the abdominal or abdomino-thoracic penetrating wounds. Patients and Methodology: Prospective study, concerning 106 patients (Sex ratio of 20,2 Mid Age of 26,2 years), observed from 01.01.2007 to 01.31.2009 at the Department of General Surgery of the Hôpital de la Libe
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Idrees, Atif, Ziyad Abdul Qadir, Komivi Senyo Akutse, et al. "Effectiveness of Entomopathogenic Fungi on Immature Stages and Feeding Performance of Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae." Insects 12, no. 11 (2021): 1044. http://dx.doi.org/10.3390/insects12111044.

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Maize is a major staple crop in China, and the sustainable productivity of this primary crop has been recently threatened by fall armyworm (FAW), Spodoptera frugiperda, invasion. The five fungal isolates, Aspergillus sp. BM-3 and SE-2-1, Cladosporium tenuissimum SE-10, Penicillium citrinum CTD-24, and Beauveria bassiana ZK-5 were assessed for their efficacy in causing mortality against first to sixth instar eggs and neonate larvae seven days post-treatment, and their effects on the feeding performance of sixth instar S. frugiperda larvae at 48 h post-treatment at three concentrations (1 × 106,
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5

Vasconcelos, G. L., R. Maculan, N. Alves, et al. "102 In Vitro Embryo Production and Oocyte Quality in Bos indicus Beef Cows Selected for Fertility Characteristics." Reproduction, Fertility and Development 30, no. 1 (2018): 190. http://dx.doi.org/10.1071/rdv30n1ab102.

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Embryo production may be enhanced when associated with cows selected on the basis of fertility markers, which should be easy to measure, such as antral follicle count (AFC) and genital tract morphometrics. The objective was to evaluate the effects of AFC class on oocyte 24-h outcome and in vitro embryo production in Bos indicus beef cows. Brahman (n = 151) cows (2-13 years old, 344-803 kg of BW, and 7-9 BCS). Low (LAFC), intermediate (IAFC), and high (HAFC) antral follicle classes were defined as follows: LAFC ≤ 30; IAFC 30-49; and HAFC ≥50 AFC. All follicles ≥3 mm in diameter were aspirated b
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RAJKOVIC, ANDREJA, MIEKE UYTTENDAELE, SYLVIE-ANNE OMBREGT, ELINA JAASKELAINEN, MIRJA SALKINOJA-SALONEN, and JOHAN DEBEVERE. "Influence of Type of Food on the Kinetics and Overall Production of Bacillus cereus Emetic Toxin." Journal of Food Protection 69, no. 4 (2006): 847–52. http://dx.doi.org/10.4315/0362-028x-69.4.847.

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Potato puree and penne pasta were inoculated with cereulide producing B. cereus 5964a and B. cereus NS117. Static incubation at 28°C proved these two foods to be a better substrate for higher cereulide production (4,080 ng/g in puree and 3,200 ng/g in penne were produced by B. cereus 5964a during 48 h of incubation) compared with boiled rice (2,000 ng/g). This difference occurred despite B. cereus counts of more than 108 CFU/g in all three products. Aeration of cultures had a negative effect on cereulide production, causing concentrations more than 10-fold lower than in some statically incubat
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7

Zhang, P. G., and J. C. Sutton. "High temperature, darkness, and drought predispose black spruce seedlings to gray mold." Canadian Journal of Botany 72, no. 2 (1994): 135–42. http://dx.doi.org/10.1139/b94-018.

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Gray mold caused by Botrytis cinerea Pers.:Fr. developed in seedlings of black spruce (Picea mariana BSP) that were subjected to high temperatures (30–45 °C) in darkness or to drought conditions immediately before inoculation with the pathogen (106 conidia/mL). Incidence and density of spore production of B. cinerea in needles of the treated seedlings increased, and chlorophyll content decreased, with duration of the preinoculation treatments. Gray mold did not develop in seedlings subjected to preinoculation high temperature in the light, and also failed to develop in seedlings that were kept
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8

Laser, Kai T., Nikolaus A. Haas, Markus Fischer, et al. "Left ventricular rotation and right–left ventricular interaction in congenital heart disease: the acute effects of interventional closure of patent arterial ducts and atrial septal defects." Cardiology in the Young 24, no. 4 (2013): 661–74. http://dx.doi.org/10.1017/s1047951113000978.

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AbstractBackgroundLeft ventricular rotation is physiologically affected by acute changes in preload. We investigated the acute effect of preload changes in chronically underloaded and overloaded left ventricles in children with shunt lesions.MethodsA total of 15 patients with atrial septal defects (Group A: 7.4 ± 4.7 years, 11 females) and 14 patients with patent arterial ducts (Group B: 2.7 ± 3.1 years, 10 females) were investigated using 2D speckle-tracking echocardiography before and after interventional catheterisation. The rotational parameters of the patient group were compared with thos
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9

Kodom, Piyabalo, Antonio J. Aragón-Barroso, Edem K. Koledzi, Kwamivi Segbeaya, Jesús González-López, and Francisco Osorio. "Microwave Treatment of Three Different Types of Sewage Sludge Based on Their Solar Drying Exposure Time: Effect on Microorganisms, Water Content and Agronomic Aspects." Water 16, no. 2 (2024): 321. http://dx.doi.org/10.3390/w16020321.

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This study aimed to treat sewage sludge through microwave irradiation at a laboratory scale. The objective was to investigate the effect of microwave irradiation on microorganisms, water content, organic matter, and agronomic nutrients present in sewage sludge. Three types of sewage sludges obtained from a full-scale wastewater treatment plant were considered: Sludge A (raw sludge), Sludge B (subjected to 15 days of solar exposure, achieving 48% dryness), and Sludge C (exposed to solar conditions and left open to the air for 23 months, reaching 94% dryness). These diverse sludges were exposed
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10

Bi, Feng, Yanqiao Zhang, Yabing Guo, et al. "First-line iparomlimab and tuvonralimab (QL1706) or iparomlimab (QL1604) + bevacizumab (BEV) for unresectable hepatocellular carcinoma (HCC): Updated results from the phase Ib/II DUBHE-H-106 study." Journal of Clinical Oncology 43, no. 4_suppl (2025): 579. https://doi.org/10.1200/jco.2025.43.4_suppl.579.

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579 Background: Novel treatment options for unresectable HCC are needed. Iparomlimab and tuvonralimab are anti-PD-1 and anti-CTLA-4 antibodies, respectively. The DUBHE-H-106 study aims to assess safety and efficacy of first-line QL1706 or QL1604 + BEV for HCC. Preliminary data have been reported on 2023 ASCO Annual Meeting. Here, we report updated results. Methods: This study consists of three cohorts. Systemic therapy-naive adult patients (pts) with HCC, ≥ one measurable untreated lesion per RECIST v1.1, BCLC stage B–C, Child-Pugh score ≤ 7, not amenable to or progression after locoregional t
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11

Alsarahni, Aseel, Zuhair Muhi Eldeen, Elham Al-kaissi, and Hiba Al-malliti. "IN VITRO MICROBIAL TIME-KILLING CURVE FOR NEWLY SYNTHESIZED AMINOACETYLENIC-2-MERCAPTOBENZOTHIAZOLE COMPOUND." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 10 (2017): 125. http://dx.doi.org/10.22159/ijpps.2017v9i11.21245.

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Objective: To determine the time needed for killing different types of microorganisms by a newly synthesized 2-mercapto-1,3-benzothiazole derivative in comparison to ciprofloxacin and fluconazole.Methods: The minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) for 2-{[4-(2,6-dimethylPiperidin-1-yl)but-2-yn-1-yl]Sulfanyl}-1,3-benzothiazole(AZ3) compound were determined, using the broth dilution method. The MBC and MFC dilutions were prepared. Broth cultures of Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Escherichia coli (E. coli), and Pseu
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12

Turner, Dan, Bluma Brenner, Daniela Moisi, et al. "Nucleotide and Amino Acid Polymorphisms at Drug Resistance Sites in Non-B-Subtype Variants of Human Immunodeficiency Virus Type 1." Antimicrobial Agents and Chemotherapy 48, no. 8 (2004): 2993–98. http://dx.doi.org/10.1128/aac.48.8.2993-2998.2004.

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ABSTRACT We have compared nucleotide substitutions and polymorphisms at codons known to confer drug resistance in subtype B strains of human immunodeficiency virus type 1 (HIV-1) with similar substitutions in viruses of other subtypes. Genotypic analysis was performed on viruses from untreated individuals. Nucleotide and amino acid diversity at resistance sites was compared with a consensus subtype B reference virus. Among patients with non-subtype B infections, polymorphisms relative to subtype B were observed at codon 10 in protease (PR). These included silent substitutions (CTC→CTT, CTA, TT
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13

Kurniawan, Muhammad Haris, Berta Putri, and Yeni Elisdiana. "EFEKTIVITAS PEMBERIAN BAKTERI Bacillus polymyxa MELALUI PAKAN TERHADAP IMUNITAS NON SPESIFIK UDANG VANNAMEI (Litopenaeus vannamei)." e-Jurnal Rekayasa dan Teknologi Budidaya Perairan 7, no. 1 (2018): 739. http://dx.doi.org/10.23960/jrtbp.v7i1.p739-750.

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The addition of Bacillus polymyxa bacteria in the feed as immunostimulant is one of the efforts of disease prevention on vannamei shrimp (Litopenaeus vannamei). The aimed of this research was to know the effectivity of the use of Bacillus polymyxa bacteria in feed towards the non-specific imunity of vannamei shrimp. This research consisted of 4 treatments namely feed with the density of Bacillus polymyxa bacteria 0 cell/ml as control (A), 104 cell/ml (B), 106 cell/ml (C) and 108 cell/ml (D) and each treatment is repeated 3 times. This research has been done in 15 days. Parameters that observed
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14

Fournel, P., A. Vergnenégre, G. Robinet, et al. "Induction (ICT) or consolidation chemotherapy (CT) with cisplatin (C) and paclitaxel (P) plus concurrent chemo-radiation (CT/TRT) with cisplatin and vinorelbine (V) for unresectable non-small cell lung cancer (NSCLC) patients (pts): Randomized phase II trial GFPC-GLOT-IFCT 02–01." Journal of Clinical Oncology 24, no. 18_suppl (2006): 7048. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.7048.

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7048 Background: Concurrent CT/TRT is the standard treatment for unresectable stage III NSCLC, but the optimal sequencing of TRT and CT is not well defined.Consolidation CT with taxane seems to be a good approach (SWOG 95–04). Methods: Unresectable stage III NSCLC pts (weight loss < 10%, ECOG PS 0–1, no supraclavicular lymph node or superior vena cava syndrome) were eligible. in Arm A, pts received 2 cycles of C 80 mg/m2 and P 200 mg/m2 followed by a concurrent CT/TRT including TRT as 66 Gy in 33 fractions and C 80 mg/m2 d1,29 and 57 and V d1,8,29,36,57 and 64. In Arm B, the same CT/TRT beg
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15

Baiswar, P., S. Chandra, R. Kumar, S. V. Ngachan, A. R. Roy, and D. N. Upadhayay. "First Report of Leaf Blight of Chinese Ground Orchid (Phaius tankervilliae) Caused by Botrytis cinerea in India." Plant Disease 92, no. 11 (2008): 1586. http://dx.doi.org/10.1094/pdis-92-11-1586c.

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Chinese ground orchid (Phaius tankervilliae Banks:Blume) is a beautiful, terrestrial orchid, which belongs to the family Orchidaceae. It is used as a cut flower, which lasts for 4 to 5 weeks. This species is considered endangered and rare in nature. In June of 2007, potted plants of P. tankervilliae in Shillong, Meghalaya (northeast India; maximum temperature 24°C, minimum temperature 18°C, and 83.5% relative humidity) exhibited leaf blight. Symptoms included water-soaked lesions and dense, gray mold growing on infected tissues. Thirty-six percent of the plants surveyed were found to have this
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16

Laudanski, Krzysztof, Ahmed Sayed Ahmed, Mohamed A. Mahmoud, Mohamed Antar, and Hossam Gad. "Heterogeneity of the Immunological and Pathogenic Profiles in Patients Hospitalize Early Versus Late During an Acute Vital Illness as Shown in Native SARS-CoV-2 Infection." International Journal of Molecular Sciences 26, no. 5 (2025): 2349. https://doi.org/10.3390/ijms26052349.

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The immune system’s response to an invading pathogen is the critical determinant in recovery from illness. Here, we hypothesize that the immune response will swiftly follow classical activation and a resolution trajectory in patients with the rapid evolution of symptoms if challenged by a viral pathogen for the first time. Alternatively, a dysregulated response will be signified by a protracted clinical trajectory. Consequently, we enrolled 106 patients during the first wave of COVID-19 and collected their blood within 24 h, 48 h, 7 days, and over 28 days from symptoms onset. The pathogenic bu
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Pessoa, G. A., J. M. Trentin, A. P. Martini, et al. "180 SPERM SELECTION OF STALLION PONIES THROUGH GLASS WOOL." Reproduction, Fertility and Development 26, no. 1 (2014): 204. http://dx.doi.org/10.1071/rdv26n1ab180.

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Two techniques of sperm concentration (centrifugation or filtering) and sperm separation technique with glass wool were applied to the sperm samples collected from 3 pony stallions (6 ejaculates; 2 from each stallion). Ejaculates were extended to a final concentration of 50 × 106 spermatozoa mL–1 using a nonfat dry milk-based extender and evaluations occurred at 24, 48, and 72 h after immediate ejaculate dilution and cooling. Each stallion was considered as a block, and semen from each stallion was assigned to 4 treatments: Group A (control): extended semen alone; Group B: extended-centrifuged
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Rodrigues de Santana, Fabiana, Cidéli de Paula Coelho, Thayná Neves Cardoso, et al. "Modulation of inflammation response to murine cutaneous Leishmaniasis by homeopathic medicines: Antimonium crudum 30cH." Homeopathy 103, no. 04 (2014): 264–74. http://dx.doi.org/10.1016/j.homp.2014.08.006.

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Background: Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. The modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.Methods: We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 × 106 Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes prese
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Ross, Lisa L., Marjorie D. Robinson, Giampiero Carosi, et al. "Impact of HIV subtype on response and resistance in antiretroviral-naïve adults comparing treatment with once daily versus twice daily ritonavir boosted fosamprenavir in combination with Abacavir/Lamivudine." Drugs and Therapy Studies 2, no. 1 (2011): 1. http://dx.doi.org/10.4081/dts.2012.e1.

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The impact of HIV-1 subtype on resistance mutation selection and on virologic response to fosamprenavir in combination with once-daily (QD) versus twice-daily (BID) dosing of ritonavir was examined in a prospective, open label, randomized study in antiretroviral-naïve, HIV-1 infected subjects. We studied APV109141 compared QD fosamprenavir/ritonavir (1400mg/100mg) to BID fosamprenavir/ritonavir (700mg/100mg), administered in combination with a QD fixed-dose abacavir/lamivudine (600 mg/300 mg) combination tablet through 48 weeks in ART-naïve subjects. HIV genotypes were obtained from all subjec
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Crepel, C., S. Inghelbrecht, S. Baeyen, and M. Maes. "First Report of Cochliobolus sativus on Guzmania sp. in Belgium." Plant Disease 90, no. 10 (2006): 1361. http://dx.doi.org/10.1094/pd-90-1361a.

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In 2001 and again in December 2005, an outbreak of leaf spots was observed on Guzmania sp. ‘Gwendolyne’ (Bromeliaceae) in a Belgian nursery. Typical disease symptoms were irregular spots with a grayish center and a narrow red-brown margin. Identification was based on morphological characteristics and molecular techniques. Isolations of diseased leaf tissues previously washed with sterile distilled water on potato dextrose agar (PDA) resulted in mycelial colonies after 7 to 8 days. Fungal mycelium grew at a linear rate of 30.4 mm per 24 h at 21°C in the dark. The pathogen produced aerial myceli
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Rojanaporn, Duangnate, Taweevat Attaseth, Wimwipa Dieosuthichat, et al. "Clinical Presentations and Outcomes of Retinoblastoma Patients in relation to the Advent of New Multimodal Treatments: A 12-Year Report from Single Tertiary Referral Institute in Thailand." Journal of Ophthalmology 2020 (September 10, 2020): 1–13. http://dx.doi.org/10.1155/2020/4231841.

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Purpose. To investigate the clinical presentations and outcomes of retinoblastoma in relation to the advent of new multimodal treatments in Thailand. Patients and Methods. Retrospective case series. We evaluated the clinical presentation, staging, details of treatment, and treatment outcomes of retinoblastoma patients who were treated at Ramathibodi Hospital, Bangkok, Thailand, between January 1, 2007, and December 31, 2018. The log-rank test was used to explore clinical characteristics and treatment modalities that affected globe salvage and survival curves. Results. This study included 124 e
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Najafova, Gulyaz, та Gulshan Aghayeva. "Molecular Mechanics Simulation and Ftir Spectrocopy Optimization of Secondary Structure of Aβ (25-35) Peptide". SCIENTIFIC WORK 18, № 9 (2024): 45–52. http://dx.doi.org/10.36719/2663-4619/106/45-52.

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Abstract In this study, we probed the secondary structure of a soluble Aβ (25-35) peptide in water solution by applying a molecular mechanics method as well as infrared spectroscopy. We have used Fourier Transformed Infrared Spectroscopy (FTIR) to examine the secondary structure of this peptide. The amide I bands of Ab (25-35) obtained from transmission-FTIR spectra consists of one main band at 1658 cm-1, at both concentrations used (200 mM and 1 mM). This band shows us that Ab (25-35) in aqueous solution was mostly organized into a a-helical structure (48 %). The contribution of the unordered
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23

Ivors, K. L., L. W. Lacey, D. C. Milks, et al. "First Report of Boxwood Blight Caused by Cylindrocladium pseudonaviculatum in the United States." Plant Disease 96, no. 7 (2012): 1070. http://dx.doi.org/10.1094/pdis-03-12-0247-pdn.

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In September and October 2011, a new disease was observed on Buxus spp. in North Carolina and Connecticut, respectively. In North Carolina, over 10,000 containerized Buxus sempervirens (American boxwood) were affected at one location. A few weeks later, the disease was found in Connecticut on entire plantings of B. sempervirens ‘Suffruticosa’ (English boxwood) at two residential properties, and shortly thereafter on over 150,000 plants at two production nurseries. Initial foliar symptoms appeared as light to dark brown spots, often with dark borders. Spots enlarged and coalesced, often with a
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24

Peng, Pan-Pan, Liang-Liang Dong, Ya-Fang Sun, et al. "The structure of allophycocyanin B fromSynechocystisPCC 6803 reveals the structural basis for the extreme redshift of the terminal emitter in phycobilisomes." Acta Crystallographica Section D Biological Crystallography 70, no. 10 (2014): 2558–69. http://dx.doi.org/10.1107/s1399004714015776.

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Allophycocyanin B (AP-B) is one of the two terminal emitters in phycobilisomes, the unique light-harvesting complexes of cyanobacteria and red algae. Its low excitation-energy level and the correspondingly redshifted absorption and fluorescence emission play an important role in funnelling excitation energy from the hundreds of chromophores of the extramembraneous phycobilisome to the reaction centres within the photosynthetic membrane. In the absence of crystal structures of these low-abundance terminal emitters, the molecular basis for the extreme redshift and directional energy transfer is
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Palupi, Tantri, Satriyas Ilyas, Muhammad Machmud, and Dan Eny Widajati. "Peningkatan Mutu Fisiologis dan Daya Simpan Benih serta Ketahanan Patogen dan Agen Hayati pada Benih Padi Berpelapis." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 44, no. 3 (2017): 242. http://dx.doi.org/10.24831/jai.v44i3.12755.

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<p><em></em><em>ABSTRACT<br /><br />Xanthomonas oryzae pv. oryzae (Xoo), is a seedborne pathogen causing bacterial leaf blight (BLB) disease, and reduces the quality of seed and rice production. One of the efforts to control the BLB disease and to improve the quality Xoo infected seeds is the seed coating technique enriched with biological agents. The experiment was aimed to study the effect of coating on seed quality and storage life, as well as the Xoo and biological agents resistence (P. diminuta A6 and B. subtilis 5/B) on the seeds. The experiment was ca
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Harari, Alexandre, Pierre-Alexandre Bart, Wolfgang Stöhr, et al. "An HIV-1 clade C DNA prime, NYVAC boost vaccine regimen induces reliable, polyfunctional, and long-lasting T cell responses." Journal of Experimental Medicine 205, no. 1 (2008): 63–77. http://dx.doi.org/10.1084/jem.20071331.

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The EuroVacc 02 phase I trial has evaluated the safety and immunogenicity of a prime-boost regimen comprising recombinant DNA and the poxvirus vector NYVAC, both expressing a common immunogen consisting of Env, Gag, Pol, and Nef polypeptide domain from human immunodeficiency virus (HIV)-1 clade C isolate, CN54. 40 volunteers were randomized to receive DNA C or nothing on day 0 and at week 4, followed by NYVAC C at weeks 20 and 24. The primary immunogenicity endpoints were measured at weeks 26 and 28 by the quantification of T cell responses using the interferon γ enzyme-linked immunospot assay
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Muraki, Yasushi, Toshio Murata, Emi Takashita, Yoko Matsuzaki, Kanetsu Sugawara, and Seiji Hongo. "A Mutation on Influenza C Virus M1 Protein Affects Virion Morphology by Altering the Membrane Affinity of the Protein." Journal of Virology 81, no. 16 (2007): 8766–73. http://dx.doi.org/10.1128/jvi.00075-07.

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ABSTRACT Reverse genetics has been documented for influenza A, B, and Thogoto viruses belonging to the family Orthomyxoviridae. We report here the reverse genetics of influenza C virus, another member of this family. The seven viral RNA (vRNA) segments of C/Ann Arbor/1/50 were expressed in 293T cells from cloned cDNAs, together with nine influenza C virus proteins. At 48 h posttransfection, the infectious titer of the culture supernatant was determined to be 2.51 × 103 50% egg infectious doses/ml, which is lower than the number of influenza C virus-like particles (VLPs) (106/ml) generated usin
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Mariotti, E., S. Di Francesco, M. De Blasi, et al. "284 FERTILIZING CAPACITY OF BUFFALO (BUBALUS BUBALIS) SPERM CO-CULTURED WITH OVIDUCT EPITHELIAL CELLS." Reproduction, Fertility and Development 22, no. 1 (2010): 299. http://dx.doi.org/10.1071/rdv22n1ab284.

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The overall in vitro embryo production efficiency in buffalo is hampered by the poor IVF efficiency. The aim of this work was to evaluate whether the fertilizing ability of buffalo sperm is improved by the presence of bovine oviductal cells (BOEC) during IVF. Because of limited availability of buffalo oocytes, this was assessed by heterologous IVF. Bovine oviducts were obtained at a local abattoir from cows that were in the preovulatory phase of a normal estrous cycle. BOEC recovered from 5 oviducts as previously described (Gualtieri and Talevi 2000 Biol. Reprod. 62, 1754-1762) were pooled and
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Ostrowsky, Belinda, Emily Snavely, Sarah Kogut, et al. "491. Working Together: A Tale of Carbapenemase-Producing Organism Investigations in Three New York City Nursing Homes." Open Forum Infectious Diseases 6, Supplement_2 (2019): S239. http://dx.doi.org/10.1093/ofid/ofz360.2510.

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Abstract Background New York State Department of Health (NYSDOH) and Wadsworth Center (WC) participate in the Centers for Disease Control and Prevention’s Antibiotic Resistance Laboratory Network (AR Lab Network), including identification and characterization of specific bla genes in carbapenemase-producing organisms (CPO). Three investigations from November 2018–March 2019 illustrate the findings and challenges investigating CPO in a blaKPC endemic setting. Methods NYSDOH WC testing includes organism identification, drug susceptibility testing, detection of carbapenemase production, detection
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Ramallo, C. J., L. D. Ploper, M. Ontivero, M. P. Filippone, A. Castagnaro, and J. Díaz Ricci. "First Report of Colletotrichum acutatum on Strawberry in Northwestern Argentina." Plant Disease 84, no. 6 (2000): 706. http://dx.doi.org/10.1094/pdis.2000.84.6.706b.

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Isolates were obtained from strawberry tissue with anthracnose symptoms from several locations near Tucumán, Argentina. Isolates were characterized using several criteria. Isolates produced fusiform conidia, tapered to a point at both ends, and averaged 13.5 × 4.9 μm. On potato dextrose agar, colonies produced a white cottony mycelial colony that turned orange in older cultures. Compared with Colletotrichum fragariae, the new isolates produced fewer appressoria. Pathogenicity tests were conducted on detached leaves and plants in the greenhouse and field. Detached immature leaves of cvs. Chandl
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31

Gonzales, R., M. Rosales, F. Perea, et al. "98CONCEPTION RATES USING BRAHMAN BULL SEMEN FROZEN IN MILK BASED EXTENDER CONTAINING EGG YOLK OR SOYBEAN LIPIDS; A FIELD STUDY IN A TROPICAL ENVIRONMENT." Reproduction, Fertility and Development 16, no. 2 (2004): 170. http://dx.doi.org/10.1071/rdv16n1ab98.

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The objective of this study was to examine the substitution of soybean-origin phospholipids for egg yolk in Brahman bull semen extender. Semen was frozen in 3 different low-fat milk (1%) based extenders containing 10mgmL−1 of fructose and supplemented with: 8% of whole egg yolk (Extender 1, control), 8% rectified egg yolk (egg yolk granules were removed by double centrifugation at 3000g for 1h at 5°C; Extender 2), and 7.3mgmL−1 of phospholipids of soybean-origin containing 10% of phosphatidyl choline (Extender 3). All 3 extenders were supplemented with 1000IU of penicillin, 1mgmL−1 streptomyci
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Magalhaes, Renata Ferreira, Luiza Helena Urso Pitassi, Marizete Salvadego, Aparecida Machado Moraes, Maria Lourdes Barjas-Castro, and Paulo Eduardo Ferreira Velho. "Bartonella Henselae Survives after the Storage Period of the Red Blood Cell Units." Blood 110, no. 11 (2007): 2906. http://dx.doi.org/10.1182/blood.v110.11.2906.2906.

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Abstract Introduction: The genus Bartonella comprises a unique group of emerging, Gram-negative, facultative intracellular bacteria, which can cause Carrión’s disease, trench fever, cat scratch disease and bacillary angiomatosis. Man is a reservoir host of Bartonella species. Ten to 15% of the populations in areas that are hyperendemic for Carrión’s disease are asymptomatic carriers of B. bacilliformis. In addition, positive serologic tests for B. henselae were found in 2 to 6% of Americans, 48% of Swiss, 19% of Germans, and 13.7% of Brazilians. Blood donors can be asymptomatic carrier of Ba
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Kope, H. H., S. F. Shamoun, and C. Oleskevich. "First Report of Colletotrichum gloeosporioides on Arceuthobium tsugense subsp. tsugense in Canada." Plant Disease 81, no. 9 (1997): 1095. http://dx.doi.org/10.1094/pdis.1997.81.9.1095b.

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Although reports of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. occurring as a hyperparasite of western hemlock dwarf mistletoe (Arceuthobium tsugense (Rosend.) G.N. Jones subsp. tsugense) have been previously published (1), there are no collection or herbarium records to support this finding (B. Geils, personal communication). Given this ambiguity, this report is the first record of C. gloeosporioides on A. tsugense subsp. tsugense based on voucher collections deposited at the Pacific Forestry Centre (DAVFP #25277). Western hemlock dwarf mistletoe is a parasitic plant th
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34

Raza, S., KK Kundu, and SK Dutta. "Prevalence of asymptomatic pharyngel carriage of B-hemolytic Group A Streptococcus pyogens among school going children of age 5-12 years in Bharatpur, Nepal." Journal of Kathmandu Medical College 2, no. 1 (2014): 18–20. http://dx.doi.org/10.3126/jkmc.v2i1.10537.

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Background: β- haemolytic Group A Streptococcus pyogens infection is a common cause of bacterial pharyngitis among children. Children are the target population for pharyngitis as well as other suppurative and non-suppurative infections.Objectives: The objectives of this study are to find out the rate of asymptomatic throat carriage of Streptococcus pyogens and to study antibiotic susceptibility pattern of the isolates.Methods: Total 106 randomly selected children between five to 12 years were included in this study. Throat swabs collected were inoculated on 5% sheep blood agar and incubated fo
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35

Palmucci, H. E., and P. E. Grijalba. "Leaf Spot and Stem Blight Caused by Botrytis cinerea on Poinsettia in Argentina." Plant Disease 89, no. 12 (2005): 1359. http://dx.doi.org/10.1094/pd-89-1359c.

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Poinsettia (Euphorbia pulcherrima Will. ex. Klotzsch) is a worldwide potted or landscape ornamental plant that belongs to the Euphorbiaceae family. During 2003 and 2004, several symptoms were observed on poinsettia potted plants in nurseries and crops near Buenos Aires. Symptoms included irregular, brown, water-soaked spots on adult plants and leaf spots that extended causing stem blight in seedlings. Small pieces of diseased tissues were surface disinfected for 2 min in 2% sodium hypochlorite, plated on 2% potato dextrose agar (PDA), and incubated at 22°C for 48 h. Dense, whitish mycelia deve
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36

Liu, Tianyu, Nattida Boonpeng, Kang Li, et al. "The Efficacy of a Novel Selection of Bacillus spp. on Reducing Off-Flavor Compounds and Improving Flesh Quality." Water 17, no. 9 (2025): 1357. https://doi.org/10.3390/w17091357.

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Geosmin (GSM) and 2-methylisoborneol (2-MIB), microbial-derived terpenoid compounds prevalent in aquaculture systems, impair fillet quality and disrupt physiological homeostasis in aquatic species by inducing oxidative stress and lipid peroxidation. Despite their significant impact, effective strategies for eliminating these compounds from fish tissues remain underexplored. In this study, we employed primer-mediated PCR amplification to identify strains that produce 2-MIB and GSM and evaluated the efficacy of Bacillus licheniformis strain BL23 (BL23) in suppressing S. thermocarboxydus (ST), a
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Islam, MM, KKI Khalil, MA Islam, KM Sujan, A. Mustari, and MA Miah. "Effects of aloe vera (Aloe barbadensis Miller) gel on growth performance and haemato-biochemical profiles in broiler chickens." Bangladesh Veterinarian 36, no. 1-2 (2019): 25–32. http://dx.doi.org/10.3329/bvet.v36i1-2.55747.

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The study was designed to assess the effects of aloe vera gel (AVG) on broiler growth performance and haemato-biochemical changes. “Lohman” day-old broiler chicks (n=48) were reared for 28 days. At day 13, chicks were randomly divided into four equal groups (12/group). Group A was non-treated control. Group B was supplemented with antibiotic (oxytetracycline hydrochloride) as a growth promoter in water. Group C and D were supplied AVG (1% and 2%) in drinking water. Antibiotic and AVG treated broilers had higher (P<0.05) live weight (1803.8 ± 63.6 gm and 1800.00 ± 64.2 gm) than control (1607
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38

Zhang, C. Q., and B. C. Xu. "First Report of Canker on Pecan (Carya cathayensis) Caused by Botryosphaeria dothidea in China." Plant Disease 95, no. 10 (2011): 1319. http://dx.doi.org/10.1094/pdis-05-11-0457.

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In the late 1990s, sporadic occurrence of Botryosphaeria canker on Carya cathayensis was recorded in Zhejiang Province, China. From 2005 to 2009, nearly 90% of orchards in Zhejiang and Anhui provinces were seriously affected by this disease. Symptoms were similar to those of canker of C. illinoinensis (2); small, elliptical lesions that developed on the bark at points of infection and then enlarged to form large, sunken, elongated cankers. The cankers coalesced, forming large diffuse areas of blighted tissue, which turned black. Tissue samples from the margin of trunk lesions from 35 different
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39

Kay, Neil E., Ann K. Strege, Tait D. Shanafelt, Nancy D. Bone, and Yean K. Lee. "CLL B Cell Interaction with Bone Biopsy Generated Marrow Stromal Elements Enhances Their Apoptosis Resistance in Association with an Angiogenic Switch." Blood 104, no. 11 (2004): 1914. http://dx.doi.org/10.1182/blood.v104.11.1914.1914.

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Abstract CLL B cells have significant opportunity to interact with multiple tissue sites that are critical to the leukemic cell’s ability to survive. The marrow stromal elements (MSE) in B-chronic lymphocytic leukemia (B-CLL) are very likely one of the most frequently involved in this important cell-cell interaction. We have utilized a novel bone marrow biopsy technique (Alvi S, Leuk Res, 2001) that consistently generates robust and long-lived marrow stromal elements from B-CLL patients in order to study CLL B cell apoptosis status in relation to angiogenic factors. The latter angiogenic param
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40

Farias, Christyan Paiva, Flávia Machado Croisfelt, and Milla Alves Baffi. "Qualidade microbiológica do leite cru in natura, leite cru refrigerado e leite pasteurizado comercializados na região de Uberlândia, MG." Revista Verde de Agroecologia e Desenvolvimento Sustentável 9, no. 4 (2014): 250. http://dx.doi.org/10.18378/rvads.v9i4.3002.

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Neste trabalho investigou-se a qualidade microbiológica do leite proveniente de três diferentes fornecedores (duas propriedades rurais e um laticínio de Uberlândia-MG). Foram realizadas estimativas do número de bactérias aeróbias mesófilas por Contagem Padrão em Placas (CPP) em meio PCA (Agar Padrão para Contagem) a 30 oC e do Número Mais Provável (NMP) de coliformes totais em Caldo Lactosado (Teste Presuntivo) a 35 oC por 48 h. Após esse período, as amostras positivas (turbidez no meio e produção de gás) foram inoculadas em Caldo Verde Bile Brilhante (VBB, Teste Confirmativo) a 35 oC por 48 h
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41

Norouzi, Roghayeh, Abolghasem Siadatpanah, Farzaneh Mirzaei, Ruhollah Fateh, Mohammad Khalifeh Gholi, and Seyyed Jafar Adnani Sadati. "Evaluation of Antileishmania Effect of Methanolic Extract of Dandelion Root (Taraxacum Officinale) on Leishmanaia Major Promastigotes in Vitro Techniques." Qom Univ Med Sci J 15, no. 9 (2021): 590–97. http://dx.doi.org/10.32598/qums.15.9.377.2.

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Background and Objectives: Cutaneous leishmaniasis is one of the common diseases between humans and animals that is considered a health problem in Iran and many countries around the world. Currently, glucantime is used to treat cutaneous leishmaniasis. Due to its high side effects and resistance, the use of alternative therapies, especially plants and natural compounds, has been highly recommended by researchers. The aim of this study is to investigate the effect of the methanolic extract of dandelion root (Taraxacum officinale) on Leishmania major in vitro techniques. Methods: In this experim
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42

Palmucci, H. E., and E. R. Wright. "Occurrence of Botrytis cinerea on Pelargonium spp. in Argentina." Plant Disease 90, no. 8 (2006): 1107. http://dx.doi.org/10.1094/pd-90-1107c.

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Pelargonium spp. are perennial ornamental plants used as potted plants or bedding plants in gardens. During the spring and summer of 2003, symptoms of an unknown disease appeared on florists' geranium (Pelargonium inquinans (L.) L'Herit) and ivy geranium (P. peltatum (L.) L'Herit.) adult plants growing in gardens in the suburbs of Buenos Aires. Flowers showed small, water-soaked spots that expanded and eventually blighted the petals. Brown, circular to irregular, water-soaked spots developed in leaves and advanced into the peduncules. A fungus was isolated from diseased leaf tissue on potato d
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43

Stock, Eveline O., Bela F. Asztalos, John M. Miller, et al. "High-Density Lipoprotein Particles, Inflammation, and Coronary Heart Disease Risk." Nutrients 17, no. 7 (2025): 1182. https://doi.org/10.3390/nu17071182.

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Background: Coronary heart disease (CHD) remains a leading cause of death and has been associated with alterations in plasma lipoprotein particles and inflammation markers. This study aimed to evaluate and compare standard and advanced lipid parameters and inflammatory biomarkers in CHD cases and matched control subjects. We hypothesized that incorporating advanced lipid and inflammatory biomarkers into risk models would improve CHD risk prediction beyond the standard lipid measures. Methods: CHD cases (n = 227, mean age 61 years, 47% female) and matched controls (n = 526) underwent fasting bl
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Malinowski, Konrad, Michał Ebisz, Robert F. LaPrade, and Marcin Mostowy. "Platelet-Rich Plasma in Anterior Cruciate Ligament Quadriceps Tendon Bone Reconstruction—Impact of PRP Administration on Pain, Range of Motion Restoration, Knee Stability, Tibial Tunnel Widening and Functional Results." Applied Sciences 11, no. 9 (2021): 3993. http://dx.doi.org/10.3390/app11093993.

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Background: Using Platetet-Rich Plasma (PRP) in anterior cruciate ligament reconstruction (ACLR) has been suggested to improve patient outcomes. The aim of this study was to assess the impact of PRP administration on pain, range of motion (ROM) restoration and the functional results of ACLR performed with quadriceps tendon bone (QTB) autografts. Methods: A total of 106 patients were included in this multicenter study. Fifty-two patients underwent single-bundle QTB ACLR and 54 patients underwent the same procedure with additional PRP administration. Results: Mean time of need for on-demand anal
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45

M Abu Darwish, Moayad, Mohd Zul Helmi Rozaini, Hassan I. Sheikh, and Laith A. Abdul Razzak. "The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules." Malaysian Journal of Fundamental and Applied Sciences 20, no. 1 (2024): 21–39. http://dx.doi.org/10.11113/mjfas.v20n1.3187.

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Nile tilapia is one of the major farmed fish that is often infected with Streptococcus. Oral vaccination is the most preferable technique, but the antigen is usually degraded by gastric juice. Encapsulation by alginate could be effective in protecting the antigen. The aim of this study was to assess the effectiveness of vaccinating Nile tilapia using formalin-killed Streptococcus agalactiae encapsulated in alginate. The average diameter of fabricated microcapsules was 500±20 μm, with 86% encapsulation efficacy. Fish were then divided into four groups (30 fish per group): group A: fish vaccinat
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46

Alcaráz, L., M. Hidalgo, M. J. Galvez, et al. "Erratum to: 56 SINGLE LAYER CENTRIFUGATION THROUGH PURESPERM® 80 IMPROVES QUALITY OF CRYOPRESERVED DOG SPERMATOZOA." Reproduction, Fertility and Development 25, no. 1 (2013): 175. http://dx.doi.org/10.1071/rdv25n1ab56.

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Density gradient centrifugation with PureSperm® (PureSperm® 40 + PureSperm® 80; Nidacon International, Mölndal, Sweden) has been satisfactorily used to enhance the quality of dog semen samples; however, no studies have been performed on the effect of single layer centrifugation (SLC) with PureSperm® on frozen–thawed dog semen. The aim of this study was to investigate if SLC with PureSperm® 80 can improve the post-thaw semen quality of dog. Semen from 5 dogs was collected by digital manipulation. Two ejaculates from each dog were centrifuged with Tris-based extender, supernatant was removed, an
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47

Quispe Gutierrez, Ulises Sandro, Teodosio Huanca Mamani, and Luis Vicente Olivera Marocho. "INFLUENCIA DE LAS HORMONAS FOLÍCULO ESTIMULANTE, LUTEINIZANTE Y GONADOTROPINA CORIÓNICA EQUINA EN LA MADURACIÓN IN VITRO DE OVOCITOS Y CLIVAJE DE EMBRIONES DE ALPACA." Revista de Investigaciones 8, no. 1 (2019): 974–85. http://dx.doi.org/10.26788/riepg.v8i1.774.

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La producción de embriones in vitro de alpacas está aún en etapas iniciales. El objetivo fue evaluar la influencia de hormonas folículo estimulante (FSH), luteinizante (LH) y gonadotropina coriónica equina (eCG) en la maduración in vitro de ovocitos y clivaje de embriones de alpacas. Realizado en el Laboratorio de Fertilización in vitro del CIP Quimsachata, Estación Experimental ILLPA Puno. Los ovocitos se recuperaron de ovarios de alpaca faenadas, los de categoría A y B fueron madurados en medio TCM – 199 (Incubadora a 6.1% de CO2; 5% de O2; 38.5 °C con alta humedad), en grupos de tratamiento
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48

Calixto, Rodolfo, Fabiana Ostronoff, Mauricio Ostronoff, et al. "Primed-GCSF BMT After Reduced Intensity Conditioning (RIC) In Association with Rituximab for Patients (pts) with Refractory CD20+ Aggressive Lymphomas.." Blood 116, no. 21 (2010): 1313. http://dx.doi.org/10.1182/blood.v116.21.1313.1313.

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Abstract Abstract 1313 Auto-SCT for pts with refractory aggressive lymphomas is often not effective and most pts die of progression of disease. RIC has been used to treat these pts, but there is still a high relapse rate especially within the first year after the transplant. Administration of Rituximab during the first year of allo-BMT may help avoid relapse in the first year while allowing sufficient time for optimal GVL effect. Primed-GCSF BMT has shown to accelerate the neutrophil recovery and decrease the incidence of chronic GVHD when compared to PPBSCT without affecting the graft-versus-
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49

Cirvilleri, G., A. Bonaccorsi, A. Vitale, I. Castello, G. Polizzi, and S. Stefani. "First Report of Leaf Spot and Blight of Strelitzia reginae Caused by Burkholderia gladioli in Italy." Plant Disease 90, no. 12 (2006): 1553. http://dx.doi.org/10.1094/pd-90-1553b.

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During the summer of 2005, a new disease of bird of paradise (Strelitzia reginae Aiton) was observed on young seedlings (2 to 3 months old) in a nursery located in Giarre (Catania) in eastern Sicily. Symptoms included brown, water-soaked leaf spots that first appeared after seedling emergence and then gradually enlarged and became necrotic. Occasionally, during wet conditions, seedlings were completely blighted, resulting in total loss. The disease was observed on 10% of the 3,000 plants present in one nursery. A single bacterial colony was consistently isolated on King's medium B (KB) supplem
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50

Becker, F., W. Kanitz, G. Nurnberg, and D. Rath. "25 TIMED ARTIFICIAL INSEMINATION ENABLES HIGH FERTILIZATION RATES IN NORMAL CYCLING AND IN SUPEROVULATED CATTLE WITH REDUCED DOSAGES OF UNSORTED SPERMATOZOA AND WITH SEXED SPERMATOZOA." Reproduction, Fertility and Development 25, no. 1 (2013): 159. http://dx.doi.org/10.1071/rdv25n1ab25.

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Different factors determine the effectiveness of the use of sires in AI. Most important factors are the number of inseminated spermatozoa, the quality of spermatozoa, and the time of insemination. Especially in superovulated animals, the insemination scheme plays in important role to cover the whole ovulation period. The influence of 3 different dosages of spermatozoa (15 × 106, 5 × 106, and 1 × 106) on fertilization rate was examined in experiment A. In experiment B, one dosage of female and male spermatozoa of 3 different bulls was used for timed AI in 31 heifers. Timed AI in normal-cycling
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