Relevant bibliographies by topics / Porcine kidney

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Porcine kidney'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Porcine kidney"

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de Souza, Fernanda Rocha, Maria Aparecida Dalboni, Andreas Kaasi, José Osmar Medina de Abreu Pestana, Adalberto Ramón Vieyra, and Nádia Karina Guimarães de Souza. "Rapid Protocol of Porcine Kidney Decellularization." Journal of Biomimetics, Biomaterials and Biomedical Engineering 38 (August 2018): 67–74. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.38.67.

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Chronic kidney disease is a problem that has grown in recent decades worldwide. The National Kidney Foundation (NKF) estimates that the number of patients will double in the next 10 years. Dialysis and kidney transplantation are the treatments used for chronic kidney disease. There is hope in slowing down chronic kidney disease or even stopping its progression. Bioengineering and cell therapy are the main fields in kidney regeneration research using three-dimensional matrices in which cells are cultured, an ideal solution for scarcity organs for kidney transplantation. The difficulty in re-creating a functional kidney due to the complexity of its three-dimensional structure and its composition of different cell types and that can be incorporated in vivo with low immunogenicity is a very difficult task. Therefore, the aim of the present study was to meet the enormous demand for new treatments, developing strategies of tissue engineering on the basis of the decellularization of the porcine kidney performed through a new cell removal protocol. We determined the effective removal of cells by histologic and immunohistochemical analyses, showing the preservation of type IV collagen and fibronectin. Therefore, this method is a quick way to obtain decellularized porcine kidneys for future recellularization studies.
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Johnson, Blake, Scott Campbell, and Naira Campbell-Kyureghyan. "Characterizing the Material Properties of the Kidney and Liver in Unconfined Compression and Probing Protocols with Special Reference to Varying Strain Rate." Biomechanics 1, no. 2 (September 7, 2021): 264–80. http://dx.doi.org/10.3390/biomechanics1020022.

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The liver and kidneys are the most commonly injured organs due to traumatic impact forces applied to the abdomen and pose a challenge to physicians due to a hard-to-diagnose risk of internal bleeding. A better understanding of the mechanism of injury will improve diagnosis, treatment, forensics, and other fields. Finite element modelling is a tool that can aid in this understanding, but accurate material properties are required including the strain rate dependency and the feasibility of using animal tissue properties instead of human. The elastic modulus in a probing protocol and the elastic modulus, failure stress, and failure strain in a compression protocol were found for both liver and kidney tissue from human and porcine specimens at varying strain rates. Increases in the elastic modulus were seen for both the human kidney and liver, but only for the porcine kidney, when comparing the unconfined compression and probing protocols. A strain rate dependency was found for both the liver and kidney properties and was observed to have a larger saturation effect at higher rates for the failure stress than for the elastic modulus. Overall, the material properties of intact liver and kidney were characterized, and the strain rate dependency was numerically modelled. The study findings suggest that some kidney and liver material properties vary from human to porcine tissue. Therefore, it is not always appropriate to use material properties of porcine tissue in computational or physical models of the human liver and kidney.
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Abolbashari, Mehran, Sigrid M. Agcaoili, Mi-Kyung Lee, In Kap Ko, Tamer Aboushwareb, John D. Jackson, James J. Yoo, and Anthony Atala. "Repopulation of porcine kidney scaffold using porcine primary renal cells." Acta Biomaterialia 29 (January 2016): 52–61. http://dx.doi.org/10.1016/j.actbio.2015.11.026.

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Huang, Jianni, George Bayliss, and Shougang Zhuang. "Porcine models of acute kidney injury." American Journal of Physiology-Renal Physiology 320, no. 6 (June 1, 2021): F1030—F1044. http://dx.doi.org/10.1152/ajprenal.00022.2021.

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Pigs represent a potentially attractive model for medical research. Similar body size and physiological patterns of kidney injury that more closely mimic those described in humans make larger animals attractive for experimentation. Using larger animals, including pigs, to investigate the pathogenesis of acute kidney injury (AKI) also serves as an experimental bridge, narrowing the gap between clinical disease and preclinical discoveries. This article compares the advantages and disadvantages of large versus small AKI animal models and provides a comprehensive overview of the development and application of porcine models of AKI induced by clinically relevant insults, including ischemia-reperfusion, sepsis, and nephrotoxin exposure. The primary focus of this review is to evaluate the use of pigs for AKI studies by current investigators, including areas where more information is needed.
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Guan, Yong, Shuangde Liu, Yuqiang Liu, Chao Sun, Guanghui Cheng, Yun Luan, Kailin Li, Jue Wang, Xiaoshuai Xie, and Shengtian Zhao. "Porcine kidneys as a source of ECM scaffold for kidney regeneration." Materials Science and Engineering: C 56 (November 2015): 451–56. http://dx.doi.org/10.1016/j.msec.2015.07.007.

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Milicevic, Dragan, Zlatan Sinovec, Snezana Saicic, and Dubravka Vukovic. "Occurrence of ochratoxin A in feed and residue in porcine liver and kidney." Zbornik Matice srpske za prirodne nauke, no. 108 (2005): 85–93. http://dx.doi.org/10.2298/zmspn0508085m.

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The effect of ochratoxin A in feed for pigs, and the incidence of its residue in liver and kidney were investigated. The samples were taken from farms and corresponding slaughterhouse in different areas of Serbia. The criteria for OTA residue examination in the mentioned tissues were macroscopic alterations in kidneys, that is a marked kidney ischemia. 14 feed samples, 12 kidney samples and 12 liver samples in total were examined. The average OTA concentration in feed was 25,24 g/kg (0,0-85 g/kg). The presence of the OTA residue was found in all of examined tissues samples. The average OTA concentration in kidneys was 2,37 g/kg (1,0-8,2 g/kg), in liver was 2,66 g/kg (1,2-5,5 g/kg). The experiment showed that the average OTA concentration in feed of farm A in contrast to farm B was significantly low (p < 0,05), in liver was significantly lower (p < 0,01), while in kidneys was not significantly low (p < 0,05). The correlation between these three findings was postulated and discussed.
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Kumar Kuna, Vijay, Sanchari Paul, Bo Xu, Robert Sjöback, and Suchitra Sumitran-Holgersson. "Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney developmentRunning title: Regeneration of porcine kidneys." AIMS Cell and Tissue Engineering 3, no. 1 (2019): 26–46. http://dx.doi.org/10.3934/celltissue.2019.1.26.

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Milicevic, Dragan, Verica Juric, Aleksandra Dakovic, Miljan Jovanovic, Srdjan Stefanovic, and Zoran Petrovic. "Mycotoxic porcine nephropathy and spontaneous occurrence of ochratoxin A residues in kidneys of slaughtered swine." Zbornik Matice srpske za prirodne nauke, no. 116 (2009): 81–90. http://dx.doi.org/10.2298/zmspn0916081m.

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In order to find information on the occurrence of mycotoxic porcine nephropathy in Serbia, during a six month period (2006/2007) samples of kidney from individual healthy slaughtered pigs were collected (n=90) and analyzed by HPLC for ochratoxin A. In addition, histological examinations were carried out. The incidence of OTA in kidney was 33,3% and varied between 0.17-52.5 ng/g. Histopathological examination of kidneys confirmed tubulopathies with oedema and cell vacuolization. In addition, hemorrhages and necrosis of proximal kidney tubules cells were found. These findings indicate that it is likely that most of the kidney injury is related to ochratoxin A and other nephrotoxic compounds which enhance the toxicity of OTA.
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Gulik, Thomas M. van. "Appraisal of the porcine kidney autotransplantation model." Frontiers in Bioscience E4, no. 4 (2012): 1345–57. http://dx.doi.org/10.2741/e464.

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Bäcker, A., D. Bokemeyer, and H. J. Kramer. "Endothelin synthesis and receptors in porcine kidney." Acta Physiologica Scandinavica 171, no. 1 (January 2001): 105–12. http://dx.doi.org/10.1046/j.1365-201x.2001.00789.x.

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Dissertations / Theses on the topic "Porcine kidney"

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Poornejad, Nafiseh. "Decellularization and Recellularization Processes for Whole Porcine Kidneys." BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6554.

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Concern over kidney disease has increased dramatically in recent years within the medical community. It is estimated that approximately one in fifteen Americans, nearly 20 million people, experience chronic kidney disease with most of those diagnosed progressing to kidney failure. The ultimate treatment available for end stage renal failure is whole kidney transplantation. However, there are very few kidneys available for patients to receive and those patients who are fortunate enough to receive an organ must remain on immunosuppressive medication for the remainder of their lives. The United States Department of Health & Human Services have reported that 18 people die every day while on the waiting list for organ donations. The treatment is fairly successful as 69% of patients who receive a kidney transplant are still alive 5 years after the transplant. Tissue engineered organs could be a promising alternative for whole organ transplantation. The overall objective is to repopulate appropriate decellularized scaffolds from pigs, which are not immunogenic, with a patient's own cells to achieve a functional organ. Therefore, there would be an inexhaustible source of organs ready for transplantation without the risk of immune rejection. The naturally obtained scaffolds devoid of immunogens are a potential matrix to create artificial kidneys. Repopulation of decellularized rat kidneys with renal progenitor cells has been reported in previous studies. This dissertation reports the scale-up of the previous technology and building of partially functional human-sized kidneys. In the first step, we investigated various cell lysing agents and developed an automated decellularization procedure for whole porcine kidney decellularization. We also developed a preservation method for native and decellularized kidneys to avoid spoilage before and after decellularization. We also developed a decontamination procedure for whole porcine kidneys. Finally, we recellularized whole porcine kidney scaffolds with renal epithelial cells and achieved partial repopulation of the renal structure.
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Sedigh, Amir. "Management of Ischemia and Brain Death-Associated Injuries in Porcine Kidney Grafts." Doctoral thesis, Uppsala universitet, Transplantationskirurgi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-222020.

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Organs from deceased donors after brain death (BD) remain the major source of organs for transplantation. The catastrophic event of BD and the inevitable consequences of ischemia reperfusion injury (IRI) are linked to impaired graft quality and transplantation outcome. The aim of this thesis was to create a BD model in pigs to assess early effects on IRI in kidneys preserved with an oxygenated solution and to evaluate the protective effects of coating the renal vessel walls with a heparin conjugate during hypothermic machine perfusion (HMP). Brain death was achieved by raising the intracranial pressure (ICP) through stepwise increasing the volume of an epidurally placed balloon to the point of exceeding the mean arterial pressure (MAP) creating a negative cerebral perfusion pressure (CPP). This reproducible, clinically relevant experimental model makes evaluation of potential targeted methods to protect the organs possible. Kidneys retrieved from brain-dead pigs were preserved either in an oxygenated emulsion composed of 75% histidine-tryptophan-ketoglutarate (HTK) and 25% perfluorohexyloctane F6H8 or HTK alone. After 18h of cold storage the kidneys were transplanted into allogeneic pigs. F6H8 was associated with replenishment of adenosine triphosphate and lower gene expression of hypoxia inducible factor (HIF)-1a, vascular endothelial growth factor (VEGF), interleukin (IL)-1α and tumour necrosis factor (TNF)-α. F6H8 reduced early IRI at both the cellular and molecular level. Kidneys from BD pigs were evaluated for the feasibility of coating the vessel walls with the heparin conjugate CHC (Corline Systems AB, Uppsala, Sweden) to restore glycocalyx. Porcine kidneys were preserved by HMP for 20h with 50 mg biotinylated CHC added to the perfusion solution. CHC was detected on the inner surface of the kidney vessels by immunofluorescence, and its uptake in kidneys was confirmed by reduced content in the perfusate. An ex vivo normothermic perfusion circuit was developed to assess kidney function. Perfusion with CHC during HMP was associated with lower creatinine levels, increased urine volume and reduced tubular injury. Modifying renal vessels walls using CHC during HMP improved early graft function. Preservation with the oxygenated F6H8 solution or CHC could be used to improve graft quality and ameliorate IRI in kidneys retrieved from deceased donors.
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Harper, Simon John Francis. "Investigation of renal ischaemia reperfusion injury using an isolated haemoperfused porcine kidney model." Thesis, University of Leicester, 2008. http://hdl.handle.net/2381/29898.

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The aim of this study was to design and validate an isolated kidney haemoperfusion system while investigating the effects of three key factors influencing early graft injury and function; leucocyte activity, warm ischaemic time (WIT) and perfusion pressure.;Porcine kidneys were perfused with normothermic oxygenated autologous blood on an isolated organ perfusion system (IOPS) designed using cardiopulmonary bypass technology. Physiological and biochemical parameters were measured throughout the 6 hour perfusion period. Interval serum, urine and tissue samples were taken for physiological analysis, histological evaluation and assays measuring oxidative tissue injury, apoptosis and endovascular injury.;Kidneys perfused with leucocyte-depleted blood functioned significantly better than those perfused with whole blood in terms of creatinine clearance, oxygen consumption, acid-base homeostasis and renovascular haemodynamics. Haemoperfused kidneys demonstrated functional deterioration in parallel with increasing periods of warm ischaemia (7, 15, 25 and 40 minutes). Increasing WIT was also associated with elevated serum markers of oxidative protein and lipid injury and these correlated accurately with functional parameters. In contrast, elevated caspase 3 activity was associated with better renal function. A higher perfusion pressure of 95mmHg was associated with significantly improved renal function compared to sub-physiological pressures without increasing endovascular injury.;The IOPS represents a reliable and versatile model of IRI and as such has demonstrated that leucocyte depletion, WIT and perfusion pressure significantly affect early graft injury and function. The system offers extensive scope as a tool for evaluating IRI ameliorating interventions and in clinical organ viability assessment and preservation.
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Maru, Isafumi. "STUDIES ON STRUCTURE, FUNCTIONS, AND INDUSTRIAL UTILIZATION OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY." Kyoto University, 2001. http://hdl.handle.net/2433/150795.

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Bagul, Atul. "The effects of erythropoietin (EPO) and carbon monoxide (CO) on renal ischaemia/reperfusion (I/R) injury in an isolated porcine kidney model." Thesis, University of Leicester, 2009. http://hdl.handle.net/2381/7343.

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Kidney transplantation remains the best modality for renal replacement therapy, the key problem being lack of suitable organs for transplantation. Thus renewed interest in non-heart-beating-donor (NHBD) organs is on the increase to bridge this gap. NHBD organs are subjected to ischaemia-reperfusion (I/R) injury; the underlying pathophysiology, a complex inter-related sequence leading to short and long term renal allograft dysfunction. The aim of the study was to validate normothermic resuscitation perfusion (NP) as a preservation model followed by investigating the use of erythropoietin (EPO) and carbon-monoxide (CO). Porcine kidneys were perfused with normothermic-autologus blood on an isolated-organ perfusion system (IOPS), design based on cardio-pulmonary bypass technology. Renal haemodynamics and functions were then measured during 3hr reperfusion. NP restored renal blood flow and improved renal function, as assessed by % serum creatinine fall, area under curve (AUC) of serum creatinine. EPO when added to NP did not seem to add any major benefit other than marginally improve oxygen consumption. Carbon monoxide delivered as carbon monoxide-releasing molecule-3 (CORM-3) was able to improve urine output, renal blood flow, reduce intrarenal resistance as well as improve renal function reflected by significant improvement in AUC of creatinine clearance. Normothermic resuscitation preservation not only reversed some of the deleterious effects of I/R injury, it also plays an important role as a versatile delivery system to assess various manipulatory agents that have potential in ameliorating I/R injury. This study provides further evidence that CO may be protective in renal perfusion injury and supports the use of low-dose CO releasing molecules as a method of CO delivery. Thus CORM-3 has the potential application in the field of NHBD kidney transplantation, which continues to be an expanding source of transplant kidneys. While EPO did not add any major benefits when used as a manipulating agent, may have its shortfall when applied to a NHBD kidney programme.
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Nemours, Stéphane. "Identification of time- and sex-dependent pathways involved in renal ischemia-reperfusion injury in a porcine model. Link to renal cancer." Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/670696.

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Les malalties renals es deriven de defectes congènits, lesions renals agudes (AKI) o malalties renals cròniques (CKD), entre altres causes. La lesió renal d’isquèmia /reperfusió (IRI), que es troba en moltes situacions clíniques, és una de les causes principals de l’AKI que causen lesions i mort de cèl·lules epitelials del túbul proximal (PTEC). La gravetat de l’AKI i la capacitat de regenerar-se després de la lesió són determinants importants de la morbiditat i mortalitat dels pacients en un entorn hospitalari. Els homes són més propensos a la malaltia renal aguda i crònica i a avançar fins a la malaltia renal en fase final (ESRD) que les dones i actualment s’accepta que els andrògens, i no l’absència d’estrògens, són responsables d’això. S’accepta que la regeneració per PTEC supervivent és el mecanisme predominant de reparació/regeneració després de lesions tubulars isquèmiques al ronyó adult de mamífer. Les PTEC són també el lloc on s’origina el carcinoma de cèl·lules renals de cèl·lules clares (ccRCC) en humans. El ccRCC també presenta diferències de sexe, amb els homes que tenen gairebé el doble de la incidència de les dones a nivell mundial. Això va conduir a la hipòtesi que la regeneració després de lesions renals i el desenvolupament de ccRCC podrien compartir repertoris d’expressió gènica similars. Els andrògens són molt rellevants en el desenvolupament dels ronyons, cosa que suggereix que la regeneració i el càncer en les cèl·lules del túbul proximal poden recapitular, en part, els programes dependents dels andrògens en el desenvolupament del ronyó. En aquest projecte, hem volgut trobar dianes que participin en la regeneració renal i en processos de càncer renal. A més, ens va interessar estudiar la regulació de l’hormona sexual en aquestes vies. Es van realitzar anàlisis detallades de dades transcriptòmiques d’un model porcí d’AKI. Es van determinar gens que expressaven un dimorfisme sexual a tota la IRI i es van validar dianes en mostres humanes. A més, es van determinar els conjunts de gens implicats en la IRI i es van caracteritzar de manera sexual i de temps. Vam trobar que els grups genètics relacionats amb els processos de regeneració eren més actius en les dones que en els homes. A més, la resposta immune a la lesió va ser més gran en homes que en dones. Després, van vincular els processos de regeneració amb ccRCC mitjançant la superposició entre les anàlisis del transcriptomes AKI i ccRCC. A més, hem trobat diferències importants entre els transcriptomes de ronyó de ratolí i de porc després de la lesió renal. Es va establir un model in vitro d’IRI renal i es va permetre validar parcialment les troballes in vivo. Entre d’altres, vam observar que durant la IRI renal, STAT3 està regulat per la fosforilació de diferents residus. Aquest estudi constitueix una caracterització extensiva de les diferències de sexe existents durant la IRI renal. Ofereix una plantilla per caracteritzar més les diferències de sexe en malalties renals a nivell molecular.
Las enfermedades renales se derivan de defectos congénitos, lesiones renales agudas (AKI) o enfermedad renal crónica (CKD), entre otras causas. La lesión renal de isquemia / reperfusión (IRI), que se encuentra en muchas situaciones clínicas, es una de las causas principales de AKI que causan lesiones y muerte de células epiteliales del túbulo proximal (PTEC). La gravedad de AKI y la capacidad de regenerarse después de la lesión son determinantes importantes de la morbilidad y mortalidad de los pacientes en un entorno hospitalario. Los hombres son más propensos a la enfermedad renal aguda y crónica y avanzar hasta la enfermedad renal en fase final (ESRD) que las mujeres y actualmente se acepta que los andrógenos, y no la ausencia de estrógenos, son responsables de esto. Se acepta que la regeneración por PTEC superviviente es el mecanismo predominante de reparación/regeneración después de lesiones tubulares isquémicas en el riñón adulto de mamífero. Las PTEC son también el lugar donde se origina el carcinoma de células renales de células claras (ccRCC) en humanos. El ccRCC también presenta diferencias de sexo, con los hombres que tienen casi el doble de la incidencia de las mujeres a nivel mundial. Esto condujo a la hipótesis de que la regeneración después de lesiones renales y el desarrollo de ccRCC podrían compartir repertorios de expresión génica similares. Los andrógenos son muy relevantes en el desarrollo de los riñones, lo que sugiere que la regeneración y el cáncer en las células del túbulo proximal pueden recapitular, en parte, los programas dependientes de los andrógenos en el desarrollo del riñón. En este proyecto, hemos querido encontrar dianas que participen en la regeneración renal y en procesos de cáncer renal. Además, nos interesó estudiar la regulación de la hormona sexual en estas vías. Se realizaron análisis detallados de datos transcriptómicas de un modelo porcino de AKI. Se determinaron genes que expresaban un dimorfismo sexual en toda la IRI y se validaron dianas en muestras humanas. Además, se determinaron los conjuntos de genes implicados en la IRI y se caracterizaron de forma sexual y de tiempo. Encontramos que los grupos genéticos relacionados con los procesos de regeneración eran más activos en las mujeres que en los hombres. Además, la respuesta inmune a la lesión fue mayor en hombres que en mujeres. Después, hemos vinculado los procesos de regeneración con ccRCC mediante la superposición entre los análisis del transcriptomas AKI y ccRCC. Además, encontramos diferencias importantes entre los transcriptomas de riñón de ratón y de cerdo tras la lesión renal. Se estableció un modelo in vitro de IRI renal y se permitió validar parcialmente los hallazgos in vivo. Entre otros, observamos que durante la IRI renal, STAT3 está regulado por la fosforilación de diferentes residuos. Este estudio constituye una caracterización extensiva de las diferencias de sexo existentes durante la IRI renal. Ofrece una plantilla para caracterizar más las diferencias de sexo en enfermedades renales a nivel molecular.
Kidney diseases arise from congenital defects, acute kidney injury (AKI) or chronic kidney disease (CKD), among other causes. Renal ischemia/reperfusion injury (IRI), which is faced in many clinical situations, is a major cause of AKI leading to injury and death of proximal tubule epithelial cells (PTEC). The severity of AKI and the capacity to regenerate after injury are important determinants of patient morbidity and mortality in the hospital setting. Men are more prone to acute and chronic kidney disease and to progress to end-stage renal disease (ESRD) than women and it is currently accepted that androgens, and not the absence of estrogens, are responsible for that. It is accepted that regeneration by surviving PTEC is the predominant mechanism of repair/regeneration after ischemic tubular injury in the adult mammalian kidney. PTEC are also the site where the clear cell renal cell carcinoma (ccRCC) originates in humans. ccRCC also exhibits sex differences, with males having almost twice the incidence of females globally. This led to the hypothesis that regeneration after kidney injury and ccRCC development might share similar gene expression repertoires. Androgens are very relevant in kidney development, which suggests that regeneration and cancer in proximal tubule cells might recapitulate, in part, androgen-dependent programs in kidney developmental. In this project, we aimed to find targets that participate in renal regeneration and in renal cancer processes. Moreover, we were interested to study the sex hormone regulation of these pathways. Thorough analyses of transcriptomic data from a porcine model of AKI was performed. We determined genes that expressed a sexual dimorphism throughout IRI and we validated theses targets in human samples. Furthermore, we determined the gene sets involved in IRI and characterize them in a time and sex manner. We found that gene sets related to regeneration processes were more active in females than in males. Also, the immune response at injury was higher in males than in females. Afterwards, we linked regeneration processes with ccRCC by the overlap between AKI and ccRCC transcriptome analyses. Besides, we found major differences between the mouse and the pig kidney transcriptomes upon renal injury. An in vitro model of renal IRI was established and allowed to partially validate the in vivo findings. Among others, we observed that during renal IRI, STAT3 is regulated by phosphorylation of different residues. This study constitutes an extensive characterization of the sex differences that exist during renal IRI. It offers a template for further characterization of sex differences in kidney diseases at the molecular level.
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Yates, Phillip James. "The relative effects of leukocyte depletion and nitric oxide modulation in an ex-vivo porcine normothermic perfusion model of donation after cardiac death in the kidney." Thesis, University of Leicester, 2010. http://hdl.handle.net/2381/8608.

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Introduction: The shortage of donors necessitates the use of organs from donation after cardiac death (DCD) donors. These organs demonstrate a high rate of delayed graft function (DGF) and primary non-function (PNF) when compared to those from non-DCD donors. DGF and PNF are associated with shorter graft survival times. Reperfusion injury (RI) is important in the aetiology of DGF and PNF. The roles of leukocytes and nitric oxide (NO) are pivotal in the generation of RI. The aim of this study was to assess the effect on RI of modulating leukocyte and NO levels at the time of reperfusion. Methods: This study utilised a porcine extra-corporeal normothermic perfusion model of RI. Kidneys were perfused with whole (WB) or leukocyte depleted blood (LDB) alone, or with an inducible nitric oxide (iNOS) inhibitor or NO donor. This model allows the collection of haemodynamic and functional data, as well as plasma and urine for biochemical analysis. Results: Kidneys reperfused with LDB demonstrated improved blood flow and function compared to those reperfused with WB. Initial blood flow and function in the iNOS supplemented groups was worse than in the WB/LDB perfused groups, but improved in the NO donor groups. Late blood flow plateaued in the NO donor groups but improved in the iNOS supplemented WB group. LDB and iNOS supplementation together gave poor blood flow and function throughout reperfusion. Conclusion: Depletion of leukocytes abrogates the no-reflow phenomenon and reduces the oxidative stress caused by white cell infiltration, thereby improving blood flow and function. The effects of nitric oxide and its inhibition on the endothelium, glomerulus and renal tubule during initial reperfusion are dependent upon the phase of reperfusion. Early benefits to blood flow by NO supplementation are offset by the generation of NO free radicals later after reperfusion.
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Mallet, Vanessa. "Effets de la température et d'un transporteur naturel d'oxygène au cours de la conservation en transplantation rénale." Thesis, Poitiers, 2012. http://www.theses.fr/2012POIT1407/document.

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La méthode de préservation d’organes la plus utilisée actuellement en transplantation rénale est la conservation statique en hypothermie. Cependant, ce mode de conservation induit des dommages inhérents aux lésions du syndrome d’ischémie/reperfusion (I/R). Cette étude a eu pour objectif d’identifier de nouvelles conditions de préservation des greffons, afin de limiter les lésions d’I/R, en modulant la température de conservation ou par ajout d’un transporteur d’oxygène. Nous avons utilisé deux modèles : in vitro avec des cellules endothéliales et in vivo en autotransplantation rénale chez le porc.Les résultats ont confirmé les effets délétères de la conservation à 4°C contrairement à des conservations à 19°C, 27°C et surtout 32°C, permettant d’obtenir une activité métabolique, une viabilité et une intégrité cellulaire supérieures ainsi qu’une diminution des marqueurs de l’inflammation et du stress oxydant. Nous avons aussi démontré les bénéfices d'un nouveau transporteur d’oxygène, M101, dans deux des solutions de conservation les plus utilisés, UW et HTK. L'utilisation de M101 en conservation statique permet une meilleure reprise de fonction à court terme et une réduction de la fibrose, cause principale de la perte du greffon. Enfin, nous avons montré une conservation des bénéfices de M101 à des doses réduites et déterminé que cette protection était due à une multifonctionnalité de la molécule, combinant un transporteur d’oxygène, une activité superoxyde dismutase et une taille importante (permettant de réguler la pression oncotique). Ce travail a montré de nouvelles pistes de réflexion vers une préservation, et donc une qualité, supérieure des organes à transplanter
The most used preservation method in renal transplantation is hypothermic cold storage (CS). However, this method induces damages inherent to the ischemia/ reperfusion (I /R) syndrome.My study was aimed at identifying new grafts preservation conditions, to limit I/R damage, by varying storage temperature or by adding an oxygen carrier.We used two models: in vitro with endothelial cells and in vivo in pig renal autotransplantation. The results confirmed the deleterious effects of 4°C storage in contrast to conservations at 19°C, 27°C and above 32°C, resulting in improved metabolic activity, cellular viability and integrity as well as a significant reduction in markers of inflammation and oxidative stress. Then we demonstrated the benefits of a new oxygen carrier, M101, in the two most used preservation solutions, UW and HTK. Indeed, use of M101 in CS protocols improved short-term function recovery and reduced fibrosis development, main cause of graft loss. Finally, we have shown that the benefits of M101 were preserved at lower doses and we determined that this protection was due to a multifunctionality of the molecule, combining oxygen transport, superoxyde dismutase activity and a large size (regulating oncotic pressure). This work permitted the uncovering of new concepts towards improved organ preservation and quality for transplantation
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Knight, Amanda J. "The influence of ischaemic injury and manipulation of the nitric oxide synthesis pathway on pulsatile machine perfused porcine kidneys." Thesis, University of Leicester, 2006. http://hdl.handle.net/2381/29512.

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Aims of the study: 1. To assess the use of machine perfusion in predicting the severity of injury in porcine kidneys subjected to warm ischaemic damage. 2. To assess the use of machine perfusion in predicting the severity of injury in porcine kidneys subjected to warm plus cold ischaemic damage. 3. To assess the potential therapeutic effect of nitric oxide donors and nitric oxide synthase inhibitors on porcine kidneys subjected to prolonged periods of warm plus cold ischaemia. Results: In study 1, a significant relationship between the length of WIT and IRR was found. During pulsatile MP, the IRR reduced in all WIT groups. Nitrate concentration and eNOS expression tended to become higher as the WIT increased. In study 2, the kidneys subjected to the shorter WIT demonstrated a significant linear relationship between the CIT and the IRR. This relationship was not found in the kidneys subjected to a longer WIT. As the CIT increased the nitrate concentration tended to decrease, however the eNOS expression decreased. In study 3, manipulation of the NOS pathway with NO donors and NOS inhibitors did not significantly affect the IRR or eNOS expression. Conclusions: Of the indices measured, IRR had the closest and most convincing relationship with WIT. It would seem possible that this could be used as a viability assessment measure. The fact that in all studies IRR decreased over the six hours of pulsatile MP provides positive evidence of the potential benefit of MP in minimising the deleterious effects of warm and cold ischaemia. The relationship between CIT and IRR was not so clear, but certainly when the kidneys investigated were relatively ischaemically undamaged there was a linear relationship between the two. This study did not find any evidence of an association between ischaemic damage and the NO pathway.
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Tillet, Solenne. "Effets de l'inhibition des protéases de la coagulation dans un modèle porcin d'ischémie reperfusion rénale." Thesis, Poitiers, 2014. http://www.theses.fr/2014POIT1412.

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La pénurie d’organes est un problème majeur en transplantation, aussi, en France, depuis 2005 sont autorisés les prélèvements sur donneurs décédés après arrêt cardiaque (DDAC). Cependant, ces organes sont soumis à d’importantes lésions d’ischémie reperfusion, en particulier liés à la coagulation induite par la stase. La formation de microthrombi entraîne à la reperfusion un défaut d’irrigation qui aboutit à la mort celullaire. Parallèlement, l’endothélium rénal amplifie la coagulation par l’expression du facteur tissulaire. Ce processus engendre une synthèse accrue de molécules pro-inflammatoires via les PAR (Protease-Activated-Receptors) activés par les facteurs IIa et Xa. Dans le cadre de ce travail, nous avons évalué in vivo, dans un modèle porcin d’autotransplantation rénale, l’effet de deux molécules anticoagulantes de synthèse, l’une anti-Xa et l’autre combinant un effet anti-Xa et IIa. Le modèle préclinique était composé d’une période d’ischémie chaude suivie d’une conservation de 24h à 4°C en UW, modèle sévère mimant les lésions observées chez le DDAC. L’utilisation de ces anticoagulants en périconservation, a permis de réduire les lésions de fibrose et le l’inflammation responsables de la perte de greffon rénal à long terme. In vitro, les effets bénéfiques de la molécule anti-Xa+IIa, seraient dus à une limitation de l’activation endothéliale et une réduction de l’inflammation. En conclusion, l’ajout de ces anticoagulants durant la conservation des greffons a montré un bénéfice sur la reprise de fonction et sur la réduction des dysfonctions chroniques des greffons
Organ shortage is a major limitation for transplantation, then since 2005 the use of deceased after cardiac arrest donors (DDAC) became legal in France. However these organs undergo severe ischemia-reperfusion injury, partly due to stasis activated coagulation. Micro-thrombi impair a correct reperfusion of the implanted organ. Conversely activated renal endothelium is the cause of an amplification of coagulation. This leads to increased production of proinflammatory molecules via the PAR (protease-activated receptors) activation by coagulation factors IIa and Xa. In this work we have used a severe in vivo ischemia-reperfusion model and tested the effect of inhibitors of Xa and IIa on the outcome of renal autotransplantation. One of these synthetic molecules was an anti-Xa heparinoid, while the other was acombined of direct anti-IIa + anti-Xa heparinoid. The pre-clinical model included a sequence of warm ischemia followed by a cold storage 24 h at 4°C in UW, mimicking what happens in DDAC. The use of both molecules during peri-preservation was followed by a reduction of fibrosis and inflammation, known to cause long term kidney loss. In an in vitro model, we have shown that beneficial effects of the combined anti IIa-Xa could be the consequence of a reduction in endothelial activation and subsequent inflammation. We conclude that anti Xa, and anti Xa-IIa, use during organ conservation, is beneficial for kidney function and survival and that they may be used as protectors against chronic renal dysfunction
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Book chapters on the topic "Porcine kidney"

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Wingenfeld, Peter, Ute Gehrmann, Stefan Strübind, Thomas Minor, Wolf Isselhard, and Dietrich Volker Michalkl. "Long-Lasting Hypoxic Preservation of Porcine Kidney Cells." In Advances in Experimental Medicine and Biology, 203–12. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0182-8_22.

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van der Meer, Robert A., Pieter D. van Wassenaar, Johannes H. van Brouwershaven, and Johannis A. Duine. "Primary Structure of a PQQ Containing Peptide from Porcine Kidney Diamine Oxidase." In PQQ and Quinoproteins, 348–50. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-0957-1_51.

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Potter, Timothy M., and Stephan T. Stern. "Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells." In Methods in Molecular Biology, 157–65. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60327-198-1_16.

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Westlund, Pär. "Purification to Homogenity of an NAD Dependent 11-Hydroxythromboxane B2 Dehydrogenase from Porcine Kidney." In Prostaglandins in the Cardiovascular System, 92–98. Basel: Birkhäuser Basel, 1992. http://dx.doi.org/10.1007/978-3-0348-7262-1_14.

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Schnur, G. "Image-Guided Volume-Selective Magnetic Resonance Spectroscopy and Relaxometry: Application to the Porcine Kidney Perfused with Perfluorocarbons." In Magnetic Resonance in Nephrourology, 93–102. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78066-0_9.

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Vamvakas, S., W. Dekant, D. Schiffmann, and D. Henschler. "Characterization of an Unscheduled DNA Synthesis Assay with a Cultured Line of Porcine Kidney Cells (LLC-PK1)." In Nephrotoxicity, 749–54. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4757-2040-2_115.

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"MOLECULAR CHARACTERIZATION OF THE IRON-SULFUR PROTEIN FROM THE PORCINE KIDNEY MITOCHONDRIAL HYDROXYLASE SYSTEM." In Vitamin D, 168–69. De Gruyter, 1988. http://dx.doi.org/10.1515/9783110846713.168.

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Yasukawa, Kazuyuki, Nobuhiro Kawahara, Fumihiro Motojima, Shogo Nakano, and Yasuhisa Asano. "Porcine kidney d-amino acid oxidase-derived R-amine oxidases with new substrate specificities." In Flavin-Dependent Enzymes: Mechanisms, Structures and Applications, 117–36. Elsevier, 2020. http://dx.doi.org/10.1016/bs.enz.2020.06.007.

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"13C- and 15N-NMR Studies of Medium-Chain Acyl-CoA Dehydrogenase from Porcine Kidney." In Flavins and Flavoproteins 1993, 293–302. De Gruyter, 1994. http://dx.doi.org/10.1515/9783110885774-051.

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Nueangaudom, Arthit, Kiattisak Lugsanangarm, Somsak Pianwanit, Sirirat Kokpol, Nadtanet Nunthaboot, Fumio Tanaka, Seiji Taniguchi, and Haik Chosrowjan. "New Aspects of the Structure of d-Amino Acid Oxidase from Porcine Kidney in Solution: Molecular Dynamics Simulation and Photoinduced Electron Transfer." In Amino Acid - New Insights and Roles in Plant and Animal. InTech, 2017. http://dx.doi.org/10.5772/intechopen.68645.

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Conference papers on the topic "Porcine kidney"

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He, Xiaoming, Shawn Mcgee, James E. Coad, Paul A. Iaizzo, David J. Swanlund, Stan Kluge, Eric Rudie, and John C. Bischof. "Investigation of the Thermal and Injury Behavior During Microwave Thermal Therapy of Porcine Kidney." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32048.

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In this paper, we report on the characterization of microwave therapy of normal porcine kidneys both in vitro and in vivo. This technology is being developed for eventual use in the treatment of small renal cell carcinoma (RCC) by minimally invasive procedures. During experiments, microwave energy was applied through an interstitial microwave probe (Urologix, Plymouth, MN) to the kidney cortex with occasional involvement of the kidney medulla. The thermal histories at several locations were recorded. After treatment, the kidneys were bisected and small tissue slices were cut out at approximately the same depth as the thermal probes. The tissue slices were further processed for histological study. Both cellular injury and the area of microvascular stasis were quantitatively evaluated by histology. Absolute rate kinetic models of cellular injury and vascular stasis were developed and fit to this data. A 3-D finite element thermal model based on the Pennes Bioheat equation was developed and solved using a commercial software package (ANSYS, V5.7). The Specific Absorption Rate (SAR) of the microwave probe was measured experimentally in tissue equivalent gel-like solution. The thermal model was first validated by the measured in vitro thermal histories. It was then used to determine the blood perfusion term in vivo.
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Schneider, Caitlin, Mohammad Honarvar, Julio Lobo, Robert Rohling, Tim Salcudean, Samir Bidnur, and Christopher Nguan. "Blood pressure dependent elasticity measurements of porcine kidney ex-vivo." In 2016 IEEE International Ultrasonics Symposium (IUS). IEEE, 2016. http://dx.doi.org/10.1109/ultsym.2016.7728830.

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Amini, Rouzbeh, Alina Oltean, Vincent Barnett, Yoav Segal, and Victor H. Barocas. "Mechanical Properties of the Porcine Lens Capsule." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192664.

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Basement membranes are ubiquitous. In humans, genetic disorders in basement membranes can lead to many complications including kidney disease, skeletal muscle myopathy, hearing loss, and ocular abnormalities[1]. We hypothesize that genetic mutation of the microstructure of the lens capsule basement membrane will alter its mechanical properties. Because of its unique thickness and anatomically distinct margins, the lens capsule is the only site in the body where large-scale mechanical tests on the basement membrane can be made.
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Jiaqiu Wang, Xu Xiao, Robyn Duncan, Ioannis Karakitsios, Zhihong Huang, Helen Mcleod, and Andreas Melzer. "Thiel soft embalmed Porcine Kidney Perfusion Model for focused ultrasound therapy." In 2015 IEEE International Ultrasonics Symposium (IUS). IEEE, 2015. http://dx.doi.org/10.1109/ultsym.2015.0506.

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Shen, Ruilin, Guangtao Xu, Bo Hu, and Yuhong Wu. "A model for hypothermic perfusion of porcine kidney in vivo: Morphologic characteristics." In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6028043.

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Theisen-Kunde, Dirk, Sönke Tedsen, Veit Danicke, and Ralf Brinkmann. "Partial porcine kidney resection in vivo using a 1.92 µm fiber laser system." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/ecbo.2009.7373_1b.

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Theisen-Kunde, Dirk, Sönke Tedsen, Veit Danicke, and Ralf Brinkmann. "Partial porcine kidney resection in vivo using a 1.92 μm fiber laser system." In European Conferences on Biomedical Optics, edited by Ronald Sroka and Lothar D. Lilge. SPIE, 2009. http://dx.doi.org/10.1117/12.831886.

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He, Xiaoming, Shawn Mcgee, James E. Coad, Franz R. Schmidlin, Paul Iaizzo, David J. Swanlund, Eric Rudie, Stan Kluge, and John C. Bischof. "Investigation of the thermal and tissue injury behavior in microwave thermal therapy of the porcine kidney." In Biomedical Optics 2003, edited by Thomas P. Ryan. SPIE, 2003. http://dx.doi.org/10.1117/12.476545.

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Barnett, Andrew, Patrick W. McLaughlin, Haojun Zheng, and Jason Z. Moore. "Novel Instant Vacuum Biopsy Needle System." In ASME 2013 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/detc2013-13028.

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This paper explores the benefits of vacuum assistance to 18 gauge needle biopsy. Current biopsy methods are inefficient or have a high rate of failure, causing the need for further painful insertions. A novel needle insertion device was developed to create the vacuum in the needle. Using multiple pneumatic cylinders, a vacuum is created inside the end-cut needle by retracting the trocar while inserting the needle. Calculations were done to determine the force caused by the vacuum. Experiments inserting the needle into porcine kidney have shown that the vacuum assistance increases cutting efficiency.
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Wasley, Louise C., Andrew J. Dorner, and Randal C. Kaufman. "SYNTHESIS. PROCESSING AND SECRETION OF HUMAN FACTOR VIII IN MAMMALIAN CELLS: REQUIREMENT FOR VON WILLEBRAND FACTOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643874.

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In the plasma factor VIII exists as a complex with von Willebrand factor (vWF). The cloning of the cDNA for factor VIII has provided the ability to develop mammalian cell lines which express high levels of factor VIII by using appropriatate expression plasmids and DNA cotransformation with selectable markers. We have studied the synthesis, processing, and secretion of factor VIII expressed in baby hamster kidney cells and in Chinese hamster ovary cells by 35S-methionine pulse and chase labeling and analysis by immunoprecipitation with specific antibodies which recognize the light and heavy chains of factor VIII. In both mammalian cell lines, factor VIII is synthesized as a primary translation product of 230 kDa. A significant amount remains within the endoplasmic reticulum in a stable complex with a glucose regulated protein of 78 kDa. The remainder traverses into the Golgi compartment where it is cleaved to the heavy and light chain forms. Very shortly thereafter the mature factor VIII appears in the conditioned media as the mature heavy and light chain species. Very little single chain factor VIII is secreted into the conditioned media. The accumulation of factor VIII in the conditioned media requires the presence of vWF factor. In the absence of vWF, the factor VIII appears as unassociated heavy and light chains which are rapidly degraded. Bovine, porcine, or human 3WF all effectively stabilize human factor VIII expressed in these rodent cell lines. These results suggest the presence of vWF promotes factor VIII chain association which stabilizes the factor VIII to proteolysis.
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