Academic literature on the topic 'Potato dextrose Broth'

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Journal articles on the topic "Potato dextrose Broth"

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Clark, Trevor N., Madison Carroll, Katelyn Ellsworth, et al. "Antibiotic Mycotoxins from an Endophytic Fusarium acuminatum Isolated from the Medicinal Plant Geum macrophyllum." Natural Product Communications 13, no. 10 (2018): 1934578X1801301. http://dx.doi.org/10.1177/1934578x1801301017.

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An extract of the endophytic fungus Fusarium acuminatum (TC2-084) isolated from the Canadian medicinal plant Geum macrophyllum exhibited significant antimycobacterial activity against Mycobacterium tuberculosis H37Ra when fermented in both malt extract and potato dextrose broths. However, significant differences observed in the NMR spectra of the respective extracts led us to further investigate both. Bioassay guided fractionation revealed that lateropyrone was solely responsible for the bioactivity observed when TC2-084 was fermented in malt extract broth, while the antimycobacterial activity of the extract derived from the isolate fermented in potato dextrose broth was augmented by the presence of enniatins B, B1, and B4 in addition to lateropyrone.
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Karthick Raja Namasivayam, S., and P. Vinoth Kumar. "INFLUENCE OF GROWTH MEDIA ON PATHOGENICITY OF METARHIZIUM ANISOPLIAE ( METSCH) SOROKIN AGAINST CHILO PARTELLUS ( SWINHOE)." Journal of Biopesticides 02, no. 01 (2009): 92–93. http://dx.doi.org/10.57182/jbiopestic.2.1.92-93.

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ABSTRACT The present study was undertaken to evaluate various liquid growth media viz sabouraud dextrose, sabouraud sucrose, sabouraud maltose supplemented with yeast extract, potato dextrose and coconut water derived coniospores and blastospores on sorghum stem borer Chilo partellus ( Swinhoe)Among the media, the conidia harvested from sabouraud maltose yeast extract broth ( SMYB),potato dextrose broth and coconut water were found to be pathogenic to all the larval instars of C.partellus. However, blastospores derived from none of the media did cause mortality
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RANI, RITU, VINEET K. SHARMA, JAGJEET S. LORE, and P. P. S. PANNU. "Cultural studies on Ustilaginoidea virens, the incitant of false smut of rice (Oryza sativa)." Indian Journal of Agricultural Sciences 85, no. 7 (2015): 888–91. http://dx.doi.org/10.56093/ijas.v85i7.50113.

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A study was carried out to find out the favourable culture media for mass multiplication and the effect of carbon sources and duration on chlamydospore germination of Ustilaginoidea virens (Cook.) Tak. Different synthetic and semi synthetic solid, liquid media, substrate media were evaluated for mass multiplication of U. virens. Potato sucrose broth was the best medium for fast mycelia growth (104.80 mg) and sporulation (6.78 × 105 spores/ml), potato dextrose broth also favored mycelial growth, whereas corn meal dextrose broth was not suitable. Out of four solid media tested, maximum colony diameter (68.74 mm) and sporulation (6.86 × 105 spores/ml) was produced by potato sucrose agar followed by potato dextrose agar. Maximum sporulation was produced on substrate media based on barley seeds (5.54 × 105 spores/ml) followed by rice seeds (3.72 × 105 spores/ml) whereas rice husk based media produced minimum sporulation. Higher concentration of sucrose and dextrose at 2 per cent supported maximum germination of spores of U. virens and sucrose was found superior in inducing germination than dextrose. The results of this study help to understand the physiological and biochemical requirements for the growth and development of the pathogen, which could serve as an input in disease management to minimize the effect of false smut disease on rice.
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Dass, C., and A. Teyegaga. "Growth Suppression of Some Wood-Decay and Other Fungi by Bacillus subtilis." Australian Journal of Botany 44, no. 6 (1996): 705. http://dx.doi.org/10.1071/bt9960705.

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The antagonistic activity of an isolate of Bacillus subtilis was examined against five wood-decay fungi (Coriolus spp., Poria sp., Stereum sp., Hexagonia discopoda, and Schizophyllum commune) and five other fungi (Candida sp., Fusarium sp., Curvularia geniculata, Penicillium digitatum, and Trichoderma viride). The effect of the bacterium on the fungi was tested using aerated growth on potato dextrose agar, non-aerated (partially anaerobic) growth on potato dextrose agar, and growth in potato dextrose broth supplemented with cell-free filtrate of broth in which B. subtilis was previously cultured. All 10 fungal species were inhibited by water-soluble factors produced by the bacterium. Nine of the 10 species were adversely affected by volatiles synthesised by the bacterium. Varying degrees of inhibition were observed in the fungi in response to the presence of cell-free filtrate of B. subtilis in broth medium. The results of this study confirm the dual nature (production of antifungal volatile compounds in addition to water-soluble agents) of antagonism manifested by B. subtilis and the thermostable property of the antifungal factors it synthesises. It also broadens the range of fungi proven to be inhibited by the bacterium.
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Ferdous, Akter, Giush Uddin Ahmed Md., Firoz Alam M., Sultana Surovi, and Ahsanur Rahman M. "Evaluation of organic culture media for mass production of Trichoderma harzianum (Rifai)." International journal of Microbiology and Mycology (IJMM) 4, no. 2 (2016): 1–7. https://doi.org/10.5281/zenodo.8366228.

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Environmental contamination for excessive use of chemical protectant increasing the interest in integrated pest management and organic farming. Trichoderma harzianum (Rifai) a promising biocontrol agent fairly acceptable globally used against wide array of plant pathogens. Culture establishment and easy utilization technique of those isolates may not evident for commercialization in all level because of costly production. To cultivate the inoculums in the cheapest suitable media of organic source present study was undertaken. Mycelial growth, conidial production and biomass yield of Trichoderma harzianum were examined on four different culture media including potato dextrose agar, modified potato dextrose agar, carrot Agar, pulse sucrose agar. The medium had a significant effect on growth rate and population of the Trichoderma species. Carrot Agar was the best medium in terms of quick growth rate and spore production with at low cost. Average linear growth rate was measured after three days of inoculation and highest linear growth has found on potato dextrose agar medium followed by carrot agar. The Biomass yield also recorded as fresh weight and dry weight of inoculums from liquid culture medium and the maximum yielding of inoculums has found in potato dextrose broth and minimum in carrot broth. Published by the&nbsp;<strong>International journal of Microbiology and Mycology (IJMM)</strong>
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6

Senthamizhlselvan, P., J. Alice, R. P. Sujeetha, and C. Jeyalakshmi. "GROWTH, SPORULATION AND BIOMASS PRODUCTION OF NATIVE ENTOMOPATHOGENIC FUNGAL ISOLATES ON A SUITABLE MEDIUM." Journal of Biopesticides 03, no. 02 (2010): 466–69. http://dx.doi.org/10.57182/jbiopestic.3.2.466-469.

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ABSTRACT The two culture media tested both Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar ( SDA) recorded maximum mycelial growth in Beauveria bassiana isolates. Maximum growth was observed in the isolates of VpNlKKL 2121 (65.22mm), BbMtKKL 2107 (65.16mm), BbMdKKL 2106 (63.44mm) and FmNvKKL 2121 (62.78mm) and minimum growth was noticed in VpPmKKL 2120 (37.33mm) in PDA media. With SDA media maximum growth was observed in BbMtKKL 2107 (68.02mm) and BbMdKKL 2106 (66.91). Slowest growth was noticed in VpPmKKL 2120 (32.18 mm) .Highest spore count was noticed in B. bassiana isolates BbMtKKL 2107 (8.90 X 108 spores /ml) and BbMdKKL 2106 (8.77 X 108 spores / ml) isolates and are on par with FmNvKKL 2124 and VpPmKKL 2120 with PDA media. All other isolates had similar effect. Similarly, BbMdKKL 2106 isolate of B. bassiana in SDA media showed maximum spore count of 8.95 X 108 spores / ml and other isolates were on par with each other, except FpEvKKL 2119 which recorded least spore count of 0.52 X 108 spores / ml. Of the various fungi tested in (Potato Dextrose Broth)PDB and(Sabouraud Dextrose Broth) SDB (liquid broths) revealed that isolate BbMtKKL 2107 produced highest yield of (1.87g) followed by FpCmKKL 1526 (1.66g) and BbMdKKL 2106 (1.40g) and they were on a per with each other and remaining cultures four cultures were on par with each other. With reference to SDB media tested, highest yield was noticed in FpCmKKL 1526 (2.10g).
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Pérez-Anzúrez, Gustavo, Pedro Mendoza-de Gives, Agustín Olmedo-Juárez, et al. "First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity." Journal of Fungi 10, no. 1 (2024): 56. http://dx.doi.org/10.3390/jof10010056.

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This is the first record of the fungus Flavocillium subprimulinum in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against Haemonchus contortus L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against H. contortus, which is considered the most pathogenic parasite affecting lambs.
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Zhou, Ting, R. D. Reeleder, and S. A. Sparace. "Influence of nutrients on growth ofEpicoccum nigrum." Canadian Journal of Microbiology 42, no. 7 (1996): 647–54. http://dx.doi.org/10.1139/m96-089.

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Epicoccum nigrum is a potential biological control agent for certain plant diseases, such as white mold of bean caused by Sclerotinia sclerotiorum. To provide information that could be used to improve the production and efficacy of E. nigrum, the effects of nutrients, including specific carbohydrate sources and amino acids, on mycelial growth, sporulation, germination of conidia, and elongation of germ tubes were determined. In dual cultures of E. nigrum and S. sclerotiorum, the effects of nutrients on widths of inhibition zones between the two fungi were assessed. Standard mycological media supported faster radial growth than media with single carbohydrate sources and individual amino acids, but glutamic acid combined with maltose or dextrose was similar with respect to stimulation of sporulation when compared with media such as V8 juice and yeast extract agars. Dual culture inhibition zones were greater in certain simple media (dextrose and lysine, sucrose and lysine, and maltose and lysine) than in standard media. For germination and germ tube elongation, sucrose and maltose were superior to most other carbohydrate sources tested, and lysine and glutamic acid were superior amino acid sources. When standard broth media were compared for production of antifungal compounds by E. nigrum, both potato dextrose broth and malt extract broth were superior to Czapek solution. Culture filtrates of E. nigrum grown in potato dextrose broth were more inhibitory towards S. sclerotiorum than filtrates from malt extract cultures.Key words: biological control, white mold, Epicoccum purpurascens, antibiosis.
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Singh, Rajendra, Vishal Raghuvansi, Ajay Kumar, Kartik Rathi, and Joginder Singh. "Cost effective production of Metarhizium anisopliae (Metchnikoff) on different agricultural products." International Journal of Agricultural Invention 7, no. 1 (2022): 14–18. https://doi.org/10.46492/ijai/2022.7.1.3.

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The present investigation entitled “Cost effective production of Metarhizium anisopliae (Metchnikoff) on different agricultural products” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2020-21. In all there were eight treatments including control replicated thrice in a randomized block design. The cost effective production of Metarhizium anisopliae spore/ml (1x106) production incurred for the production of spores significantly superior of various treatments in showed the treatments Savoured dextrose broth (SDB), significantly superior was the best treatment by bringing down the Metarhizium anisopliae cost effective production on substrate for cost effective of Metarhizium anisopliae spore/ml (1x106) cost incurred for the production of spores T10 Savoured dextrose broth (SDB) was the best low cost substrate and high production showed in the table 1 (Rs 11.49 and 156.40 spore/ml), T11 Potato dextrose broth (PDB) (Rs 6.96 and 133.50 spore/ml), and solid substrate T8 Vermicompost + 1% YE + 1.0 g Dextrose (Rs 12.20 and 110.55 spore/ml).
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T. Khalaf, Kawther. "Isolation and Identification of two bioactive compounds from basidiomycetes fungus Coprinus sp." University of Thi-Qar Journal of Science 5, no. 2 (2015): 54–58. http://dx.doi.org/10.32792/utq/utjsci/v5i2.122.

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The fungus Coprinus sp. was isolated and cultivation in laboratory on two PDA medium with some growthinduction .Two bioactive chemical compounds were isolated and purified from mycelial culture of Coprinus sp. byusing potato dextrose agar and potato dextrose broth . The two compounds were identified by using GC-mass technique .The molecular weight of purified compounds 1 and 2 were 352 KD and 388 KD respectively and chemical formula of compound 1 is C21H24N2O3 which isolated from solid medium while compound 2 is C24H26O4 which isolated from broth medium . The antibacterial activity of the purified compounds against three bacterial species E. coli , Proteus sp. and S. aureus were tested by using a disk diffusion agar method reaching to 40 , 30 , 20 for compound 1and 37, 25 , 17 mm for compound 2.
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Book chapters on the topic "Potato dextrose Broth"

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Suman, K., and Lathika Das. "Isolation of Yeast and Extraction of Superoxide Dismutase and ß Glucanase and Its Application in Agriculture." In Convergence of Technology & Biology - Transforming Life Sciences. Shanlax Publications, 2025. https://doi.org/10.34293/ctbtls.2025.ch020.

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Enzymes are highly efficient biocatalysts researched for industrial-scale catalysis because of their several distinct advantages. The present research includes the extraction of enzymes that have agricultural applications from yeast. The yeast cells were used to produce enzymes that had agricultural applications. The yeast cells were first isolated from two different curd sources. Isolation was done by serial dilution followed by the spread plate technique was employed for the collected curd samples. The isolates were grown on specific media like Yeast Potato Dextrose (YPD) medium. Cultural, and morphological study was performed for the selected colonies. The isolated yeast cells were screened for the production of enzymes like superoxide dismutase and β Glucanase. These enzymes are Significant and have extensive applications in agriculture. The yeast cells were then grown on YPD broth and incubated at 30°C for 24 h in an orbital shaking incubator at 180 rpm. The cells were centrifuged and lysed with cell-specific buffer for extraction of enzymes. Qualitative and Quantitative analysis was performed on the crude enzyme suspension to understand the activity rate of superoxide dismutase and β glucanase. The main study involves the action of the enzymes, superoxide dismutase and β glucanase on seed germination and plant growth. These enzymes can be used in modern agriculture to gain tolerance to stress conditions and reduce reliance on chemical inputs, making agriculture more eco-friendly.
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Conference papers on the topic "Potato dextrose Broth"

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Vassilev, Nikolay, Luis Garcia del Moral Garrido, Vanessa Martos Nunes, Giuseppe Falvo D�Urso Labate, and Maria Vassileva. "GLYCEROL-BASED FERMENTATION BY PLANT GROWTH PROMOTING ASPERGILLUS NIGER FOR ITS FURTHER FORMULATION AND APPLICATION IN TOMATO GROWTH." In SGEM International Multidisciplinary Scientific GeoConference 24. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/6.1/s25.24.

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Sustainable crop production includes methods of growing food in a responsible manner avoiding application and dependence on chemically produced fertilizers and pesticides. The latter means development of approaches that lead to environmentally mild inputs based on the production-consumption-recycling principle. Biofertilizers are an important tool to achieve sustainable crop production. In this work, we report the results of experiments on growth and spore/mycelium production of plant growth promoting A. niger applying standard nutritional medium (potato-dextrose broth, PDB) enriched with 3% insoluble phosphate (20 to 200 mesh hydroxyapatite of animal-bone origin, HABO) and 0 to 80 g/L glycerol (a by-product of biodiesel production. Results showed the ability of A. niger to acidify the medium with the highest titratable acidity of 28.9 mmol/1 (at 5% of glycerol) and solubilize animal bone char under these conditions. As a second stage of the experimental work, the resulting final products were used to formulate gel-based inoculant. Both, the spores and mycelium produced during the fermentation process were further used as a base for formulation to make the biofertilizer production the key in the Sustainable Agriculture. Storage of the resulting products reported here was facilitated by the presence of glycerol in the formulation system.
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