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Journal articles on the topic 'Potato Micropropagation Medium'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Cioloca, Mihaela, Andreea Tican, Nina BÄƒrÄƒscu, and Monica Popa. "Effect of Combined Growth Regulators on in vitro Multiplication of Recalcitrant Potato Varieties." Romanian Agricultural Research 38 (2021): 109–16. http://dx.doi.org/10.59665/rar3811.

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The potato varieties studied in this paper were created at the National Institute of Research and Development for Potato and Sugar Beet BraÅŸov. They have a high biological production capacity and are attractive and very appreciated by farmers, potato producers, consumers and processors, but manifest recalcitrance to the in vitro culture techniques. Thus we tried to find an optimal culture medium variant for these varieties, in order to obtain healty potato seed material in a short time. The study compared the effects of five different culture media using MS medium containing various concentra

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2

Vinh, Tran Thai, H’ Yon Niê Bing, Dang Thi Tham, Nguyen Thi Thanh Hang, Vu Kim Cong, and Nong Van Duy. "Micropropagation of Paphiopedilum x Dalatense." Vietnam Journal of Biotechnology 19, no. 1 (2021): 155–63. http://dx.doi.org/10.15625/1811-4989/14550.

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Paphiopedilum x dalatense is a beautiful orchid species with large flowers in variable colors and leaves covered with stripes and beautiful unseen mosaic spots. Recently, many people exploit this species, causing it becomes very rare. In this study, we studied the effects of various organic matter: potato, banana and tryptone, yeast powder, peptone on the growth and development of P. dalatense shoots as well as the effects of NAA and humic acid on in vitro rooting of this orchid were investigated. The research results showed that MS medium supplemented with 100 g/L banana in combination with 1

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3

Venkatasalam, E. P., Jyoti Latawa, S. K. Chakrabarti, et al. "Modified medium for micropropagation of recalcitrant potato cv. Kufri Jyoti." Indian Journal of Horticulture 72, no. 4 (2015): 574. http://dx.doi.org/10.5958/0974-0112.2015.00107.3.

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4

Kujeke, G. T., T. C. Chitendera, R. T. Masekesa, et al. "Micropropagation of Livingstone Potato (Plectranthus esculentus N.E.Br)." Advances in Agriculture 2020 (June 6, 2020): 1–11. http://dx.doi.org/10.1155/2020/8364153.

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Livingstone potato (Plectranthus esculentus N.E.Br) is an underutilised indigenous root vegetable grown by communal farmers in the eastern provinces of Zimbabwe. It is vegetatively propagated using unimproved retained tubers from the previous season. The risk of disease carryover is therefore high, leading to poor yields. The objective of the study was to exploit the tissue culture technique of micropropagation to produce a mass supply of healthy planting material for improved productivity. Two experiments were conducted: firstly, to determine the best explant type and secondly, to determine t

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5

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, and Snehasis Jana. "Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Potato Micropropagation Medium." American Journal of Bioscience and Bioengineering 3, no. 5 (2015): 106–13. https://doi.org/10.11648/j.bio.20150305.24.

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Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanni

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Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Khemraj Bairwa, and Snehasis Jana. "Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Potato Micropropagation Medium." American Journal of Bioscience and Bioengineering 3, no. 5 (2015): 106–13. https://doi.org/10.5281/zenodo.168534.

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Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanni

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7

Durnikin, D. A., N. A. Kolpakov, K. Y. Guseva, and A. V. Matsyura. "In vitro micropropagation and ex vitro rooting of some potato varieties." Ukrainian Journal of Ecology 9, no. 4 (2019): 679–89. http://dx.doi.org/10.15421/2019_810.

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The article presents the results on selection of optimal concentrations of nutrient media components and nutrient solution at the stages of clonal micropropagation of potato varieties Lyubava, Kemerovochanin, and Tuleevskiy (actual reproduction, rooting in vitro, adaptation to ex vitro conditions). The influence of some components of the nutrient medium (sucrose, agar-agar, growth regulators, namely α-naphthyl acetic acid, β-indolyl acetic acid, and β-indolyl propionic acid) was studied at the stages of reproduction and rooting in order to obtain regenerants of the studied potato varieties. Th

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8

Kane, Michael E., and Charles Lane. "Micropropagation of Wetland Plants: Sagittaria latifolia." HortScience 32, no. 3 (1997): 547B—547. http://dx.doi.org/10.21273/hortsci.32.3.547b.

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Many wetland plant species used for aquascaping and wetland revegetation projects are collected from donor wetland sites for planting elsewhere. Increased demand for wetland plants has lead to over-collection and subsequent environmental damage to these donor sites. Micropropagation provides an ecologically sound alternative to field collection and allows for production of under utilized wetland species and genotypes that are either slow-growing or difficult to propagate using conventional methods. Sagittaria latifolia Willd. (Duck-potato), a rhizomatous herbaceous wetland species, was establi

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9

Fedorova, Yulia, Larisa Fedorova, and Margarita Zaitseva. "Influence of the complex of vitamins Pikovit in culture in vitro on the growth and development of potato microplants." BIO Web of Conferences 43 (2022): 02004. http://dx.doi.org/10.1051/bioconf/20224302004.

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In the conditions of the North-West zone of Russia, studies of Pikovit vitamin complex preparation were carried out. Since the main way to obtain a healthy seed is clonal micropropagation. Therefore, in the course of the work, the process and method of clonal micropropagation of Russian potato varieties using new preparations were improved, the selection of nutrient media at all stages of cultivation was carried out with the Pikovit preparation at the concentration of 0.5 to 1.5 mg/l. As a result, it was found that use of the Pikovit vitamin complex has a positive effect on the ontogenesis of

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10

Amlesom, Winta Semere, Taddese Mehari, and Brhan Khiar Saleh. "Evaluation of Different Starches as Gelling Agents for Micropropagation of Potato." Journal of Agricultural Science 13, no. 5 (2021): 144. http://dx.doi.org/10.5539/jas.v13n5p144.

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Unavailability of clean planting material is a major constraint of potato production in Eritrea. In vitro multiplication is proved to be a reliable solution; however, due to high media cost and initial investment, its use has been limited. The current study was carried out to evaluate the effectiveness of three starches (corn, potato and barley) of both laboratory and commercial grades as agar substitute, for micropropagation of potato. Single nodes of potato were sub-cultured into a fresh modified Murashige and Skoog (MS) medium supplemented with sucrose (3% w/v) and gelled with 50 g/l of six

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11

Kumar Trivedi, Mahendra. "Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Potato Micropropagation Medium." American Journal of Bioscience and Bioengineering 3, no. 5 (2015): 106. http://dx.doi.org/10.11648/j.bio.20150305.24.

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12

Barsukova, E. N., I. V. Kim, and T. N. Сhekushkina. "IMPROVEMENT AND IN VITRO MICROPROPAGATION OF POTATO VARIETIES FOR DISEASE-FREE SEED GROWING." Far Eastern Agrarian Herald, no. 4 (2018): 20–26. https://doi.org/10.22450/1999-6837-2018-4-20-26.

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The quality of potato seed material is one of the main factors determining its yield. Under the long-term reproduction potato accumulates diseases, mostly viral. Viral diseases cause the decrease in yield 2-3 times, worsen the quality of tubers. It is possible to solve this problem by creating virusresistant varieties, as well as by switching seed production to a virus-free base (healthy basis). Creation of the virus – free collection under the in vitro conditions is the basis for the development of potato seed production in any region of the Russian Federation. In this regard, the purpose of

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13

Tham, Dang Thi, H’Yon Nie Bing, Nguyen Thi Thanh Hang, et al. "Micropropagation of dendrobium heterocarpum lindl." Vietnam Journal of Biotechnology 16, no. 1 (2018): 127–35. http://dx.doi.org/10.15625/1811-4989/16/1/13457.

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Dendrobium heterocarpum Lindl. is an endangered species which is currently used as ornamental pot plant for its beautiful flowers. An increase in collection for trade or any other purposes may lead to a dramatic decrease in the population of this species, thus becoming rare or endangered species in the near future. In this study, effects of plant growth regulators (BA, NAA, IAA, IBA, TDZ) and natural supplements (carrot, potato, and banana extracts) on protocorm like bodies (PLBs) formation; growth and development of shoot; and root regeneration of D. heterocarpum Lindl. as well as type of sub

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14

A., Elmurodov,, Abdullaeva, Yu., and Abdullaeva, S. "Efficiency of Growing in vitro Seed Tubers of Potato Varieties in the Conditions of the Zarafshan Valley." Bulletin of Science and Practice, no. 1 (January 15, 2023): 173–81. http://dx.doi.org/10.33619/2414-2948/86/23.

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The article presents the results of micropropagation of potato varieties under in vitro conditions. Botanical seeds and tubers of Arnova and Picasso varieties of potatoes were taken as the basis of the objects of study. Under laboratory conditions, the minitubers obtained on the basis of meristem cells were grown in the improved nutrient Murashige and Skoog medium. Intensive reproduction is studied and efficiency is presented.

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15

Kalashnikova, E., R. Kirakosyan, V. Zaynullin, and A. Yudin. "In vitro reproduction of promising potato varieties for cultivation in the Komi Republic." Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences, no. 7 (December 22, 2023): 36–41. http://dx.doi.org/10.19110/1994-5655-2023-7-36-41.

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The paper deals with the results of clonal micropropagation of promising potato varieties for cultivation in the conditions of the Komi Republic. The study material is three potato varieties (Zyryanets, Pechorsky, Vychegodsky), characterized by the high resistance to late blight and abiotic stress factors in the conditions of the Komi Republic. The primary explants were weakly germinated eyeholes, as well as cuttings and shoot tips isolated from 14- and 30-day-old potato plants which underwent surface sterilization and then were cultivated in the Murashige–Skoog nutrient medium containing vari

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16

Fatima, Jabeen, Arshad Muhammad, Muhammad Nasir Qayyum Mir, Shah Zaman Muhammad, and Shafique Iqra. "Exploring the effects of indole butyric acid (IBA) on in vitro growth of potato (Solanum tuberosum)." Advances in Agriculture and Biology 4, no. 1 (2021): 29–33. https://doi.org/10.63072/aab.21005.

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In this study the effect of different concentration of indole butyric acid (IBA) was examined on <em>in vitro</em> propagation of potato (<em>Solanum tuberosum</em>). Three doses of IBA (1 ml, 2 ml, and 3 ml) were applied in liquid medium to potato explants of Asterix variety. The main purpose of this study was to optimize the dosage of IBA that would develop the best results for potato micropropagation. The experiments were conducted at Gilgit Baltistan's Agriculture Lab. Various parameters such as number of roots, roots length, and total weight were measured after exposing Asterix explants t

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17

Nasution, Melvi Wahyu Angraini, Lutfhi Aziz Mahmud Siregar, and Mohammad Basyuni. "Optimization medium at the propagation and formation stage of micro tubers some varieties of potato." Indonesian Journal of Agricultural Research 8, no. 1 (2025): 9–16. https://doi.org/10.32734/injar.v8i1.16798.

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Potato cultivation in Indonesia is inadequate and experiences fluctuations. One of the obstacles is the limited availability of land for seed propagation and the high cost of in vitro media. The aim of this research is to increase the efficiency of MS media (Murashige and Skoog) for cultivating several potato varieties to form micro tubers in vitro. The research was conducted at the Biotechnology Laboratory, Faculty of Agriculture, USU Medan from June to November 2023. The research was carried out by planting node cuttings from four potato cultivars (Granola, Dayang Sumbi, Atlantic Malang, and

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18

Gagarina, Irina, and Anna Popova. "The effect of quercetin on the regeneration of potato micro plants under in vitro conditions." АгроЭкоИнфо 4, no. 52 (2022): 30. http://dx.doi.org/10.51419/202124430.

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Potato biotechnology in vitro has found wide application in the primary seed production of this crop - in the technologies of rehabilitation, micropropagation, depositing varietal collections in vitro, obtaining microtuber planting material. The article considers the effect of quercetin on the growth and development of potato micro plants under in vitro conditions. The objectives of the study included the morphological assessment of potato regenerants on nutrient media of various mineral compositions, as well as the study of the response of potato mericlons to the presence of quercetin in the

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19

Nagy, Alexandra Mihaela, Paula Oros, Corina Cătană, Maria Mihaela Antofie, and Camelia Sava Sand. "In Vitro Cultivation of Purple-Fleshed Potato Varieties: Insights into Their Growth and Development." Horticulturae 9, no. 4 (2023): 425. http://dx.doi.org/10.3390/horticulturae9040425.

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Purple-fleshed potatoes (PFP) are varieties of Solanum tuberosum L., which recently have been recorded to be more and more cultivated and consumed in all European countries, including Romania, as they are promoted for their content in bioactive compounds and benefits to human health. This paper presents a micropropagation protocol study for PFP varieties already traded into the Romanian market, namely Blue Danube (BD), Salad Blue (SB), Violet Negretin (VN), and Violet Queen (VQ). These varieties were tested for in vitro micropropagation also considering asepsis, initiation, callus formation, a

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20

Klanrit, Preekamol, Khanittha Lila, Paramaporn Netsawang, Phakamas Siangsanor, Pornthap Thanonkeo, and Sudarat Thanonkeo. "Effect of Organic Additives on the Micropropagation of Asparagus officinalis." Horticulturae 9, no. 11 (2023): 1244. http://dx.doi.org/10.3390/horticulturae9111244.

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In vitro propagation is one of the most promising techniques for the large-scale clonal propagation of Asparagus officinalis. The aim of this study was to investigate the effect of organic additives, including coconut water (CW), banana homogenate (BH), and potato homogenate (PH), on A. officinalis shoot and root proliferation. The results revealed that CW, specifically at 20% (v/v), was the most effective organic additive for promoting shoot and root formation in the in vitro plantlets. Furthermore, the longest shoot and root lengths were also observed in the MS medium supplemented with 20% (

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21

Hajare, Sunil Tulshiram, Nitin Mahendra Chauhan, and Girum Kassa. "Effect of Growth Regulators on In Vitro Micropropagation of Potato (Solanum tuberosum L.) Gudiene and Belete Varieties from Ethiopia." Scientific World Journal 2021 (February 8, 2021): 1–8. http://dx.doi.org/10.1155/2021/5928769.

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Aim. Potato (Solanum tuberosum L.) is one of the important crops in Ethiopia which has a crucial role in nutritional security, poverty alleviation, and income generation. The aim of the present investigation is to develop an efficient in vitro propagation protocol for Belete and Gudiene potato varieties by using lateral bud as explants. Materials and Methods. Shoot initiation was achieved by inoculating buds on full-strength MS Murashige and Skoog medium (MS) fortified with variable concentrations of BAP and NAA. Basal MS was used as control throughout the experiment. Results. Results of our s

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22

Yessilbekova, Ye Ye, T. S. Khosnutdinova, X. O. Bogdanova, G. T. Dolanbayeva, and Ye A. Zhakmanova. "Influence of a selenium-containing stimulant on the growth, development and yield potential of potato plants (solanum tuberosum)." IOP Conference Series: Earth and Environmental Science 1010, no. 1 (2022): 012144. http://dx.doi.org/10.1088/1755-1315/1010/1/012144.

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Abstract Potato (Solanum tuberosum) is one of the most consumed crops in Kazakhstan. The basis for modern potato cultivation technology is the use of healthy and virus-free material. One of these methods is micropropagation of potato plants in vitro on a nutrient medium. The addition of stimulants to the nutrient medium allows for rapid and healthy plant growth. One of the effective growth stimulants is the selenium-containing drug DAFS 25. It is well known that selenium has a beneficial effect on plant growth, flowering, adaptation to changing environmental conditions and plant resistance to

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23

Le, Giang K. T., Tinh V. Nguyen, Ngan K. T. Tran, Vy T. H. Dang, and Phong V. Nguyen. "PLANT REGENERATION THROUGH PROTOCORM-LIKE BODIES DERIVED FROM STEM THIN LAYER OF Anubias barteri var. Nana Petite." BIOTROPIA 31, no. 3 (2024): 349–58. https://doi.org/10.11598/btb.2024.31.3.2214.

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Article Highlights- Effective induction of PLBs from stem thin layer and shoot regeneration using specific BAP and auxin combinations.- Significant role of potato extract in enhancing shoot regeneration.- Highest shoot regeneration rate achieved with 3 mg/L BAP concentration. AbstractAnubias barteri var. nana Petite, a highly valued ornamental aquatic plant in the Araceae family, faces challenges in vegetative propagation due to its slow multiplication rate. This study established a robust micropropagation protocol for rapid and efficient multiplication of A. barteri var. nana Petite. Explants

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24

Saakian, Alexander. "Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski." АгроЭкоИнфо 4, no. 46 (2021): 17. http://dx.doi.org/10.51419/20214417.

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Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms

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25

Parveen, Fazlima, Mahmuda Khatun, and Aparna Islam. "Micropropagation of Environmental Stress Tolerant Local Potato (Solanum tuberosum L.) Varieties of Bangladesh." Plant Tissue Culture and Biotechnology 24, no. 1 (2014): 101–9. http://dx.doi.org/10.3329/ptcb.v24i1.19218.

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In vitro response of four explants namely, leaf, shoot apex, nodal and internodal, in three stress tolerant Bangladeshi potato varieties, viz. Zaubilati, Shadaguti and Challisha were tested. Of all the varieties, Shadaguti responded best for all the explants. Among the explants nodal segment responded best, followed by shoot apex. For all these explants and varieties, shoot regeneration response was tested in response to two cytokines, BAP and Kn. When compared between BAP and Kn supplementation, Kn treatment performed better than that of BAP for nodal and shoot apex, while opposite was observ

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26

Lee, Yung-I. "Micropropagation of Cypripedium formosanum Hayata through Axillary Buds from Mature Plants." HortScience 45, no. 9 (2010): 1369–72. http://dx.doi.org/10.21273/hortsci.45.9.1369.

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A micropropagation protocol for an endangered slipper orchid species, Cypripedium formosanum Hayata, through axillary buds from adult plants has been developed. The season of explant collection is crucial for the initial success of an aseptic culture. Explants collected in the middle of January gave the highest percentage of explant survival (54.2%) and shoot-forming percentage (41.7%). Of the two cytokinins tested, N6-benzyladenine (BA) was found to be superior to thidiazuron for normal shoot formation. The optimum result was obtained in quarter-strength Murashige and Skoog medium containing

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Shakeela, Azam, Hussain Azhar, Shah Zaman Muhammad, Hussain Shah Sabir, and Muhammad Nasirqayyum Mir. "Effect of phytohormones on root development of potato (Solanum tuberosum L.)." Advances in Agriculture and Biology 2, no. 1 (2019): 8–13. https://doi.org/10.63072/aab.19002.

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This research study investigated the effect of phytohormones on root development in three varieties (Barna, Kuroda, and Bartina) of potato (<em>Solanum tuberosum</em> L.). The study was conducted at the Tissue Culture Laboratory, Agriculture Research Directorate, Gilgit. The Murashige and Skoog medium was used as the plant growth medium for the experiments. Different concentrations of indole-3-butyric acid (IBA) and Gibberellic acid (GA3) were supplemented to the medium to find out their effects on root development. The root length, root weight, number of roots, and total root weight were meas

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Kubota, Chieri, and Toyoki Kozai. "Growth and Net Photosynthetic Rate of Solanun tuberosum in Vitro under Forced and Natural Ventilation." HortScience 27, no. 12 (1992): 1312–14. http://dx.doi.org/10.21273/hortsci.27.12.1312.

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Growth and net photosynthetic rate of potato (Solanum tuberosum L.) `Benimaru' plantlet in vitro were studied under a conventional photomixotrophic condition [with 20 g sucrose/liter in the medium and under 70 μmol·m-2·s-1 photosynthetic photon flux (PPF)] with minimal ventilation (MV) and under photoautotrophic conditions (without sugar in the medium and under 190 μmol·m-2·s-l PPF) with enhanced natural ventilation using an air diffusive filter (DV) or with forced ventilation (FV). Fresh weight of the plantlets cultured in the FV and DV treatments was 2.4 times that of the plantlets cultured

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Niu, Genhua, Makio Hayashi, and Toyoki Kozai. "Simulation of the Time Courses of CO2 Concentration in the Culture Vessel and Net Photosynthetic Rates of Potato Plantlets Cultured Photoautotrophically and Photomixotrophically in Vitro under Different Lighting Cycles." HortScience 32, no. 3 (1997): 515F—515. http://dx.doi.org/10.21273/hortsci.32.3.515f.

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Potato (Solanum tuberosum L. cv. Benimaru) plantlets were cultured under four lighting cycles (photoperiod/dark period: 16 h/8 h, 4 h/2 h, 1 h/0.5 h, and 0.25 h/0.125 h) photoautotrophically (without sugar in the medium), and photomixotrophically (with sugar in the medium) in vitro for 28 days. Simulations of time courses of CO2 concentration in the vessel (Ci) and dry weight accumulation of the plantlets cultured photoautotrophically were conducted using a previously developed model (Niu and Kozai, 1997). While underestimation and overestimation of time courses of Ci in some treatments were o

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30

Afoussatou, ZERBO, SOME Koussao, SORO Monique, et al. "Evaluation of the Effects of Growth Media on Potato (Solanum tubérosum L.) Plantlets Production in Burkina Faso." Annual Research & Review in Biology 40, no. 7 (2025): 14–23. https://doi.org/10.9734/arrb/2025/v40i72265.

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Aims: To identify an appropriate culture medium suitable for micropropagation of plantlets with good root proliferation and stem growth of potato. Study Design: In Burkina Faso, potato growers face difficulties in obtaining potato seeds. In general, most of the imported seeds partially meet the quality requirements of potato production. Furthermore, these seeds are not accessible in terms of cost and are unavailable in time. In vitro culture can remedy this problem by providing quality seeds. Place and Duration of Study: This study was conducted in the laboratory of the Institut de l’Environne

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Novikov, O. O., M. S. Romanova, E. V. Khaksar, N. I. Leonova та E. I. Kosinova. "The eﬀects of diﬀerent compositions of growth media on the development of microplants of the Solnechny potato variety". Siberian Herald of Agricultural Science 51, № 6 (2022): 11–21. http://dx.doi.org/10.26898/0370-8799-2021-6-2.

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The results of studying the eﬀect of nutrient media of various compositions on the growth of improved micro-plants of potatoes of the Solnechny variety grown under laboratory conditions in vitro are presented. Six compositions of the nutrient medium were studied: standard Murashige-Skuga medium modiﬁed for micropropagation (considered as a control), modiﬁed Murashige-Skuga medium with a reduced content of mineral components (up to 1/2 and up to 1/3), modiﬁed Murashige-Skuga medium with an increased content of agar-agar (10 g/l), modiﬁed Murashige-Skuga medium with a reduced content of agar-aga

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32

Ciobanu, Iustina Brînduşa, Dana Constantinovici, and L. Creţu. "The Dynamics of Cell Division in Some Local Potato Varieties Cultivated in Vitro on Micropropagation Medium." Cercetari agronomice in Moldova 45, no. 3 (2012): 71–78. http://dx.doi.org/10.2478/v10298-012-0057-y.

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Abstract This study was performed to reveal the changes in cell division, as a result of the prolonged period of subculture on micropropagation medium, of five local varieties of Solanum tuberosum L. maintained on in vitro collection at Suceava Genebank, Romania. For this purpose it was used the Murashige-Skoog medium (MS- 1962) with addition of 40 g/l sucrose, and 6 mg/l daminozide. The effect of prolonged period of subculture up to two and 12 months was expressed as mitotic index and frequency of cells with abnormal division. Mitotic index ranged from 20.1 to 22.1% after 12 days, between 15.

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Grigoryan, M. A., A. A. Starchikov, O. V. Tkachenko, G. L. Burygin, and L. G. Kurasova. "Comparison of the effect of Azospirillum baldaniorum sp245 and exogenous indolyl-3-acetic acid on the growth of ten varieties of potato microclones in in vitro culture." IOP Conference Series: Earth and Environmental Science 1010, no. 1 (2022): 012162. http://dx.doi.org/10.1088/1755-1315/1010/1/012162.

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Abstract The article presents the results of a comparison of the effect of rhizospheric growth-stimulating bacteria of the Azospirillum baldaniorum Sp245 strain and exogenous indolyl-3-acetic acid on the growth of microclones of potato varieties Nevsky, Condor, Red Scarlett, Rosara, Avrora, Zhukovsky early, Ilyinsky, Vasilek, Fioletovy, Darenka at micropropagation in in vitro culture. The A. baldaniorum Sp245 strain is capable of synthesizing indolyl-3-acetic acid upon plant inoculation. Exogenous indolyl-3-acetic acid was introduced into the nutrient medium in three concentrations. The physio

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Samala, Sainiya, and Jaturon Thipwong. "Influences of Organic Additives on Asymbiotic Seed Germination of Dendrobium cruentum Rchb. f. for In Vitro Micropropagation." Trends in Sciences 20, no. 3 (2023): 4181. http://dx.doi.org/10.48048/tis.2023.4181.

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Dendrobium cruentum Rchb. f., a commercial orchid in Thailand, faces the risk of extinction and should be conserved, and propagated for commercial. Therefore, the aim of this research was to identify a suitable VW medium supplemented with organic additives for the micropropagation of D. cruentum through asymbiotic seed germination. The VW media supplemented with separate organic additives (coconut water (CW) (100 mL/L), ‘Hom Thong’ banana homogenate (BH) (1000 mg/L), and potato homogenate (PH) (1000 mg/L) or its combination was determined, and the seed germination rate (%), protocorm formation

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Schuld, B. A., M. D. Harrison, and J. Crane. "Symptomless infection with Clavibacter michiganensis subspecies sepedonicus during tissue culture propagation of potato." Canadian Journal of Plant Science 72, no. 3 (1992): 943–53. http://dx.doi.org/10.4141/cjps92-119.

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Nodal cuttings of tissue cultured potato (Solarium tuberosum L. ’Sangre’) were inoculated with the bacterial ring rot (BRR) pathogen, Clavibacter michiganensis subsp. sepedonicus (Cms). Under commercial tissue culturing conditions, the pathogen persisted through as many as three micropropagation cycles, each consisting of production of a plantlet from an apical cutting. Infected plantlets did not develop BRR symptoms of leaf wilt or chlorosis. Signs of bacterial presence, cloudiness of tissue culturing medium or foliar lesions, were not present when the inoculum level was low, 5.0 × 102 cfu mL

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Kirakosyan, Rima N., Elena A. Kalashnikova, Halid G. Abubakarov, et al. "Influence of Mineral Treatment, Plant Growth Regulators and Artificial Light on the Growth of Jewel Sweet Potato (Ipomoea batatas Lam. cv. Jewel) In Vitro." Life 13, no. 1 (2022): 52. http://dx.doi.org/10.3390/life13010052.

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Sweet potato (Ipomoea batatas (L.) Lam), a member of the bindweed family (Convolvulaceae Juss.), is well known for its food, medicinal, and industrial values. It is estimated that more than 7000 sweet potato cultivars have been bred to date. Jewel sweet potato (I. batatas Lam cv. Jewel) is one of the most popular cultivars of sweet potato grown today because of its high nutritional value, delicious taste, and is suitable for all processing methods. However, little is known about the micropropagation of jewel sweet potato. The purpose of this paper was to study the effect of three important fac

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H. Fadaladeen, Laylan, Rafail S. Toma, Ahmed A. Saheen, and Huda B. Ahmed. "A Rapid Micropropagation Protocol for Sweet Potato (Ipomoea batatas L.) Via Tissue Culture Technique." Diyala Agricultural Sciences Journal 14, no. 1 (2022): 31–39. http://dx.doi.org/10.52951/dasj.22140104.

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A rapid and reliable micropropagation protocol was developed for two sweet potato varieties, Mangawy and Mabrokat Al-Shimal, by testing various growth regulators and different carbon sources. Healthy cultures were taken from nodal segment explants taken from potted plants. Adding BA was more effective than Kinetin when used at the same concentration. The highest number of leaves per explant (5.22 leaves/ explant) and the longest shoots (2.88 cm) were achieved when 2 mg L-1 BA was added to the culture medium. For Mabrokat Al-Shimal, the highest mean length of shoots (3.77 cm) was obtained when

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Ariste, Arile, Ma del Carmen Ojeda Zacarías, Héctor Lozoya Saldaña, et al. "Optimized in vitro micropropagation and microtuber production in potato (Solanum tuberosum L.) through apical buds using hormone regulation and tissue culture techniques." Journal of Experimental Biology and Agricultural Sciences 13, no. 1 (2025): 86–96. https://doi.org/10.18006/2025.13(1).86.96.

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Potato is an essential crop worldwide, and optimizing micropropagation techniques is important for enhancing germplasm conservation and large-scale production. This study focuses on the in vitro propagation of two potato varieties, Agata and Fianna, emphasizing optimizing sterilization protocols, shoot induction, rooting, and microtuber production. Apical buds from healthy, disease-free plants were selected as explants. These buds were surface-sterilized using 70% ethanol and sodium hypochlorite (NaOCl) with Tween-20. The explants were excised from tuber sprouts and cultured on Murashige and S

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Rehana, S. Ahmed F. Zeba N. Husna A. and Hossain F. "Effect of sunlight and artificial light on micropropagation of potato (Solanum tuberosum L.) plantlets." Archives of Agriculture and Environmental Science 3, no. 2 (2018): 151–56. https://doi.org/10.26832/24566632.2018.030208.

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Fluorescent lamps (artificial light) and sunlight were used as lighting source in present study to identify feasibility of using sunlight in plant tissue culture laboratory. <em>In vitro</em>regenerated nodal segments of the cardinal variety of potato (<em>Solanum tuberosum</em> L.) were inoculated in MS medium without any growth regulator for shoot and root development. After 30 days of culture in an average temperature at 21.94±0.21°C, relative humidity 39.35±0.75%, various morphological responses i.e. plantlet height, diameter of plantlet, number of nodes per pla

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Abwe, Mercy Ngone, Monah Ndam Lawrence, Mungfu Njilar Rita, Oumar Doungous, and Eku Njock Thomas. "Effective medium for in vitro sprouting of the buds and multiplication of the plantlets, and identification of the fungal contaminant associated with the explants of Gnetum." GSC Biological and Pharmaceutical Sciences 16, no. 2 (2021): 001–13. https://doi.org/10.5281/zenodo.5195768.

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Plant tissue culture requires the optimization of growth media. <em>Gnetum,</em> known locally in Cameroon as “Eru” is an indigenous gymnospermous vegetable with diverse medicinal, nutritional, cultural and socio-economic values. This resource is over-exploited and expected to neighboring countries, resulting to increased scarcity in the forest. Preliminary work on the <em>in vitro</em> culture of nodal cuttings was faced by the problem of fungal contamination. It was therefore necessary to isolate and identify the fungal contaminant, optimize the surface steril

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Karyanti, Karyanti, Yosua Glen Kristianto, Hayat Khairiyah, et al. "PENGARUH WADAH KULTUR DAN KONSENTRASI SUMBER KARBON PADA PERBANYAKAN KENTANG ATLANTIK SECARA IN VITRO." Jurnal Bioteknologi & Biosains Indonesia (JBBI) 5, no. 2 (2018): 177. http://dx.doi.org/10.29122/jbbi.v5i2.3012.

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The Effect of Culture Container and Carbon Source Concentration on In Vitro Shoot Propagation of Atlantic PotatoABSTRACTPotato is a food commodity that has the potential to support food diversification in Indonesia. There is an increasing demand for Atlantic potatoes as the raw material for processed potato products. The demand, which has not been met by the increased production, has been the cause of the ongoing potato import activities in Indonesia. The limitation of producing quality Atlantic potato seeds economically is one of the obstacles to increasing the production of Atlantic potatoes

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Zahia, Kadi, Rached Kanouni Malika, Khammar Sara, et al. "Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro." Acta Scientifica Naturalis 7, no. 1 (2020): 8–25. http://dx.doi.org/10.2478/asn-2020-0003.

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AbstractThis work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and thei

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Kumlay, Ahmet Metin. "Combination of the Auxins NAA, IBA, and IAA with GA3Improves the Commercial Seed-Tuber Production of Potato (Solanum tuberosumL.) underIn VitroConditions." BioMed Research International 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/439259.

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The study compared the effects of1.0×MSmedium containing various concentrations ofα-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), alone or in combination with gibberellic acid (GA3) in micropropagation of three potato (Solanum tuberosumL.) cultivars Pasinler, Granola, and Caspar using binodal stem cuttings. The results testified improved regeneration on1.0×MSmedium containing variants of NAA, IAA, and IBA plus GA3on all cultivars. The minimum days to shoot induction on three cultivars ranged 4.25–5 d on1.0×MSmedium containing 0.25 mg L−1 GA3+1 mg L−

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Abubakarov, H. G., N. N. Sleptsov, A. V. Sumin, E. A. Kalashnikova, and R. N. Kirakosyan. "Influence of lightculture on obtaining planting material of Ipomoea batatas (L.) in vitro." IOP Conference Series: Earth and Environmental Science 1112, no. 1 (2022): 012112. http://dx.doi.org/10.1088/1755-1315/1112/1/012112.

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Abstract The current article presents the results of the influence of lighting conditions on the morphogenetic potential of Ipomoea batatas (L.) microcuttings in vitro. The studied objects were 4 sweet potato cultivars - Jewel, Purple, Porto Rico, Vinnitsa pink. Microcuttings of I. batatas were cultivated in vitro. We used nutrient medium with mineral salts (according to the prescription of Murashige and Skoog (MS)) and medium only with distilled water (without mineral salts). All nutrient media contained sucrose 2% and agar 0.7%. Hormones were not added to the nutrient media. We studied the i

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Nie Bing, H’Yon, Tran Thai Vinh, Vu Kim Cong, Dang Thi Tham, and Nong Van Duy. "\(\textit{In vitro}\) regeneration of \(\textit{Renanthera imschootiana}\) Rolfe from protocorm-like body." Academia Journal of Biology 45, no. 2 (2023): 47–59. http://dx.doi.org/10.15625/2615-9023/17155.

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Renanthera imschootiana Rolfe is an extremely rare and endangered tropical epiphytic orchid. Studies on in vitro culture of R. imschootiana were conducted in order to conserve and increase the genetic pool of this wild orchid species. In this study, the effect of plant growth regulators (BA and NAA), ripe bananas in combination with potato, humic acid, and silver nanoparticles (AgNPs) were investigated to find a suitable condition for in vitro plant regeneration from R. imschootiana protocorm-like body (PLB). The results showed that after 45 days of culture, MS medium supplemented with 100 g r

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Yousef, Atheel N., Gharbia H. Danial, Diaa A. Ibrahim, and Sazan E. Barakat. "PROPAGATION AND CALLUS REGENERATION OF POTATO (SOLANUM TUBEROSUM L.) CULTIVAR ‘DESIREE’ UNDER SALT STRESS CONDITIONS." Science Journal of University of Zakho 12, no. 3 (2024): 294–98. http://dx.doi.org/10.25271/sjuoz.2024.12.3.1236.

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Under laboratory conditions, segments of potato with single nodes were exposed to varying doses of Sodium Chloride (NaCl) (0 mM, 20 mM, 40 mM, 60 mM, 80 mM and 100 mM), using the Murashige and Skoog growth medium to assess how NaCl salt stress affects micropropagation, callus formation, and regeneration in the ‘Desiree’ potato plant cultivar, and also seeks to determine the ability of this cultivar to thrive in salt stress conditions. The data were collected after a six-week period for each salt treatment. Remarkably, a significant increase in the mass of green and dry stems and roots was obse

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Singh, Tejinder, V. P. S. Panghal, Lila Bora, Love Sapra, Anita Choudhary, and Bichhinna Maitri Rout. "In vitro Propagation and Genetic Assessment by using Markers in Potato (Solanum tuberosum L.) cv Kufri Ganga." Environment and Ecology 41, no. 4 (2023): 2348–57. http://dx.doi.org/10.60151/envec/olzq4325.

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The purpose of this study was to determine the best types and concentrations of plant growth regulators to use during different stages of micropropagation, as well as to assess the genetic fidelity of tissue culture raised plants of a cv Kufri Ganga using reliable markers. According to the data on shoot bud initiation, a combination of 0.25 mgl-1 BAP and 0.5 mgl-1 Kinetin results in the greatest number of shoots per explant (3.4±0.11) in the shortest amount of time (3.2±0.09) days. The maximum number of in vitro shoots per shootlet (4.7±0.15) was recorded when auxins were combined with cytokin

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Asghari-Zakaria, R., B. Maleki-Zanjani, and E. Sedghi. "Effect of in vitro chitosan application on growth and minituber yield of Solanum tuberosum L." Plant, Soil and Environment 55, No. 6 (2009): 252–56. http://dx.doi.org/10.17221/1018-pse.

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In order to investigate the effects of soluble chitosan on plantlets growth <I>in vitro</I> and increase of minituber yield in potato micropropagation, plantlets of Agria cultivar were treated <I>in vitro</I>with soluble chitosan at different concentrations including 0, 5, 15, 50, 150, 500, 750 and 1000 mg/l added to the MS tissue culture medium. Plantlets were subsequently transferred to the greenhouse and minituber yield parameters were evaluated. At the concentrations of 750 and 1000 mg/l of chitosan the culture medium failed to solidify. Application of 500 mg/l of s

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Lugovtsova, Svetlana Yurievna, and Valentina Yurievna Stupko. "Concentration and ratio of IAA and gibberellic acid as factors to affect the effectiveness of potato micropropagation." Agrarian Scientific Journal, no. 1 (February 6, 2024): 32–38. http://dx.doi.org/10.28983/asj.y2024i1pp32-38.

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Nutrient media differed in the concentration and ratio of IAA and gibberellic acid (GA3) were compared concerning their effect on the rate of cloning of potato microplants in vitro. Microplants of the Red Scarlet variety served as the object. Hormone-free Murashige-Skoog medium (MS) was used as a control. The total cultivation period was 49 days. The rate of shoot growth and the internode formation was higher by 1.5 times on the media with phytohormones already in the first week of cultivation. From the 35th day until the end of the observations, the number of internodes on I1G2 media (1 mg/l

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Rout, Bichhinna Maitri, A. K. Bhatia, Lila Bora, Shohaib Sheikh Ayub Chauhan, Love Sapra, and Tejinder Singh. "Optimization of an Efficient Micropropagation Protocol and the Assessment of Genetic Fidelity of Solanum tuberosum L. cv Kufri Neelkanth." Environment and Ecology 41, no. 3D (2023): 2145–54. http://dx.doi.org/10.60151/envec/whwi5650.

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The aim of our experiment was to determine the optimal types and concentrations of plant growth regulators used during different stages of micropropagation and assess the genetic fidelity of tissue culture raised plants of a purple colored cv Kufri Neelkanth by using reliable markers. According to the shoot bud initiation data, it was found that utilizing a combination of 0.25 mg l-1 BAP and 0.5 mg l-1 Kinetin resulted in the greatest number of shoots per explant (3.5±0.12) within a timeframe of (3.3±0.08) days. The maximum number of in vitro shoots per shootlet (10.4±0.59) were recorded when

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