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Dissertations / Theses on the topic 'Potato virus X (PVX)'

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1

Leveau, Aymeric. "Caractérisation structurale de l'éliciteur du virus X de la pomme de terre (PVX) : recherche, chez les plantes cultivées, de gènes hôtes, impliqués dans la résistance liée à Rx." Thesis, Evry-Val d'Essonne, 2012. http://www.theses.fr/2012EVRY0036.

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De nos jours, les agents pathogènes des plantes, engendrent encore des pertes agricoles importantes. A terme, la solution la plus adaptée semble être la création de variétés de plantes cultivées présentant une résistance génétique à large spectre et durable. Créer de telles plantes nécessite la compréhension des mécanismes de résistance mis en jeu dans des pathosystèmes modèles, tels que celui impliquant le gène de résistance Rx et le virus X de la pomme de terre. Dans ce système, la protéine Rx intervient dans la perception d’un facteur d'avirulence issu du virus: sa protéine de capside (Cp).
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2

Jobsri, Jantipa. "Induction of protective antigen-specific anti-tumour immunity using vaccines incorporating immunoenhancing properties of the coat protein from the potato virus X (PVX)." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/362530/.

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DNA fusion vaccines encoding the idiotypic (Id) single chain variable fragments of B-cell malignancies fused to the potato virus X coat protein (PVXCP) were shown to enhance anti Id antibody and T-cell responses which resulted in protection in lymphoma and myeloma models. I further explored the use of PVXCP to enhance induction of anti -tumour immunity. The aims of this study were to generate two types of Id tumour vaccines against the BCL1 lymphoma, a multimeric Id-PVXCP fusion protein and a PVX particle displaying Id antigen, and to compare their performance with the DNA fusion vaccine. The
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3

Werschallová, Markéta. "Mutační analýza obalového proteinu X viru bramboru (PVX)." Master's thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-258763.

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The thesis deals with the mutational analysis of conserved amino acids of Potato virus X coat protein (PVX CP). The importance of selected amino acids for the spread of the virus in the plant should be determined. Nicotiana benthamiana was selected as an experimental plant. Mutations of the PVX CP were based on the comparison of PVX CP amino acid sequence with the sequence of Papaya mosaic virus coat protein (PapMV CP), the only representative of potexvirus, which includes PVX, with the described crystal structure of the CP. The importance of certain amino acids for interaction of coat prote
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4

Brandolini, Andrea G. "Breeding potato for resistance to leafroll virus combined with immunity to PVX and PVY." Thesis, University of Reading, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302990.

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5

Cruz, Simon Santa. "Analysis of resistance to potato virus X in potato." Thesis, University of East Anglia, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359280.

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6

Chapman, Sean Nicholas. "A molecular analysis of potato virus X." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386398.

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7

Forsyth, Alexander Mark. "Resistance to potato virus X specified by the Nb gene of potato." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294657.

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8

Adams, S. E. "Studies on mechanics and occurrence of resistance of potato virus X." Thesis, Imperial College London, 1986. http://hdl.handle.net/10044/1/37916.

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9

Bendahmane, Abdelhafid. "Analysis of a gene-for-gene interaction associated with Rx-mediated resistance to potato virus X." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389350.

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10

Perraki, Artemis. "La Rémorine, une protéine végétale impliquée dans la propagation virale ; implication des modifications post-traductionnelles." Thesis, Bordeaux 2, 2012. http://www.theses.fr/2012BOR21997/document.

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Les Rémorines (REM) du groupe 1 sont des protéines spécifiques du monde végétale. Malgré leur caractère hydrophile elles sont localisées à la membrane plasmique. La phosphorylation des REM serait potentiellement impliquée dans la signalisation précoce et la défense des végétaux contre les pathogènes. Benschop et al. (2007) détecte AtREM1.3 (Arabidopsis thaliana, groupe 1b) phosphorylée en réponse au traitement par l'éliciteur générale flg22, tandis que Widjaja et al. (2008) a suggéré que la phosphorylation de AtREM1.2 est potentiellement impliquée dans la signalisation précoce à l'infection pa
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11

Davenport, Guy Francis. "A study of the RNA-dependent RNA polymerase of potato virus X and its use in transgenic resistance." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317994.

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12

Lee, Karin L. "High Aspect Ratio Viral Nanoparticles for Cancer Therapy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1467714833.

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13

Cheng, Jacqueline Kwok Lam. "Characterization of the RNA translocation of Potato Virus X (PVX) into chloroplast." 2007. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=788864&T=F.

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14

Arruda, Amanda. "Identification of a Chloroplastic "RNA Tractor" within the Genome of Potato Virus X (PVX)." Thesis, 2009. http://hdl.handle.net/1807/18149.

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Previous studies have detected the presence of the capsid protein (CP) and CP RNA of PVX in chloroplasts of transgenic plants expressing the PVX CP and 8kD genes from nuclear transgenes. CP RNA was also found in the chloroplasts of transgenic plants with mutations that eliminated either the CP or the 8kD protein. To further investigate the potential “RNA Tractor” activity of the PVX RNA, various constructs containing limited regions of the PVX 8kD and CP genes were produced and used to transform tobacco plants. RT-PCR analyses of chloroplastic RNA ascertained the presence of the RNA transcrip
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15

Ju, Ho-Jong. "Subcellular localization and role of potato virus X (PVX) TGBp2 and TGBp3 in virus movement." 2007. http://digital.library.okstate.edu/etd/umi-okstate-2168.pdf.

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16

Mitra, Ruchira. "Subcellular localization and role of Potato virus X (PVX) TGBp2 in virus cell-to-cell movement." 2005. http://digital.library.okstate.edu/etd/umi-okstate-1236.pdf.

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17

Draghici, Heidrun-Katharina. "Funktionelle Charakterisierung der Replikations- und Rekombinationsfunktionen der RNA-abhängigen RNA-Polymerase (RdRp) des Potato virus X (PVX)." Doctoral thesis, 2009. http://hdl.handle.net/11858/00-1735-0000-0006-B032-7.

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18

Mekuria, Tefera Asamenew. "A study of vascular movement of potato virus X (PVX) TGBp1, TGBp2 and CP using the Commelina yellow mottle virus promoter and GFP." 2007. http://digital.library.okstate.edu/etd/umi-okstate-2355.pdf.

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19

Draghici, Heidrun-Katharina [Verfasser]. "Funktionelle Charakterisierung der Replikations- und Rekombinationsfunktionen der RNA-abhängigen RNA-Polymerase (RdRp) des Potato virus X (PVX) / vorgelegt von Heidrun-Katharina Draghici." 2008. http://d-nb.info/994766424/34.

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20

Su, Chun-Wei, and 蘇峻暐. "Expression of Chimeric Bamboo mosaic virus Coat Protein by Potato Virus X vector." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/87923675310679024079.

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碩士<br>國立中興大學<br>生物科技學研究所<br>102<br>Foot-and-mouth disease virus (FMDV) is a major plague in animal farming. The present vaccine used is produced by chemical inactivation which may has the risk of live virus contamination. Therefore, the subunit vaccine can be considering as a safer choice for future vaccine production. Accordingly, a Bamboo mosaic virus (BaMV) based viral vector expressing FMDV epitope - BVP1 recombinant virus had been established in previous study, expressing FMDV VP1 epitope by fusing it to the N-terminal of BaMV coat protein (CP). But since the BaMV host range is relatively
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21

Bamunusinghe, Devinka C. N. "Determination of sub-cellular localization of potato virus X replicase during infection." 2008. http://digital.library.okstate.edu/etd/umi-okstate-2717.pdf.

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22

Mann, Krinpreet. "POTENTIAL COMPLEMENTATION OF POTATO VIRUS X MOVEMENT WITH GRAPEVINE RUPESTRIS STEM PITTING-ASSOCIATED VIRUS TRIPLE GENE BLOCK PROTEINS." Thesis, 2011. http://hdl.handle.net/10214/2920.

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A movement protein Potato virus X (PVX) chimera virus (PVX.GFP(CH3)) bearing the grapevine virus Grapevine rupestris stem pitting-associated virus (GRSPaV) triple gene block proteins (TGB) (denoted P1, P2 and P3) instead of the PVX TGB was delivered into N. benthamiana and other related species by agro-inoculation. This movement protein PVX chimera virus was found to be unable to support the local and systemic movement of PVX in cis. Local and systemic movement of this PVX chimera virus was restored in trans by the dianthovirus Red clover necrotic mosaic virus (RCNMV) movement protein and by a
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23

Hillhouse, Brian Jason. "Screening of biflavonoid compounds and British Columbian bryophytes for antiviral activity against potato virus X." Thesis, 2003. http://hdl.handle.net/2429/14125.

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Plant viruses are responsible for causing significant losses to the agricultural industry. Recent investigations of antiviral compounds have suggested that biflavonoids may be a group of compounds which cause powerful inhibition to a broad spectrum of viral pathogens. Due to the high content of biflavonoids reported in bryophytes, this project has screened 50 bryophyte samples which represent 41 species for Potato Virus X (PVX) inhibition using a local lesion assay in Chenopodium quinoa. As a result of this screening, bryophytes have been identified as a prominent antiviral group; 29 of the 41
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24

Hu, Bin. "Cis-acting elements important for potato virus X minus-strand RNA synthesis in vitro and in vivo." 2006. http://www.lib.ncsu.edu/theses/available/etd-02032006-151554/unrestricted/etd.pdf.

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25

Lidický, Ondřej. "Heterologní exprese onkoproteinu E7 lidského papilomaviru (HVP 16)." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-285123.

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Production of vaccines and pharmaceutical proteins in plants is a promising nascent technology with a great potential to provide high-quality, safe and non-expensive production and delivery platform. In this work we studied the experimental vaccine against human papillomavirus based on modified plant pathogen - Potato virus X (PVX). The experimental vaccine is based on PVX virus particles decorated with genetically fused HPV-E7 oncoprotein. These chimeric virus particles should be able to activate strong and specific cellular immune response. However the modification of the PVX coat protein wi
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26

Vaculík, Petr. "Biotechnologické využití rostlinných virů." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-351027.

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The latest model of tertiary structure of capsid protein of potato virus X (PVX CP) was used as a template to design new insertion sites suitable for the preparation of PVX-based antigen presentation system. Based on this model, seven insertion sites (A-G) located in putative surface loops were tested. As an antigen inserted into these sites was used 17 amino acids long epitope derived from human papillomavirus type 16 E7 oncoprotein (E7 epitope) fused with either 6xHis tag or StrepII tag in both possible orientations (6xHis-E7 and E7-6xHis, StrepII-E7 and E7-StrepII). Prior to plant expressio
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