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Journal articles on the topic 'Pratylenchus'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 June 2025

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1

TOWNSHEND, J. L. "POPULATION DENSITIES OF FOUR SPECIES OF ROOT-LESION NEMATODES (Pratylenchus) IN THE OAT CULTIVARS, SAIA AND OAC WOODSTOCK." Canadian Journal of Plant Science 69, no. 3 (July 1, 1989): 903–5. http://dx.doi.org/10.4141/cjps89-105.

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The population densities of four species of root-lesion nematodes (Pratylenchus) were examined in two cultivars of oat (Avena sativa L.) grown in greenhouse pots. The cultivar Saia harbored fewer P. neglectus and P. penetrans than the cultivar OAC Woodstock, while both cultivars were similar hosts for P. sensillatus. Pratylenchus crenatus did not develop in either cultivar.Key words: Oat, population density, Pratylenchus crenatus, Pratylenchus neglectus, Pratylenchus penetrans, Pratylenchus sensillatus
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2

Majd Taheri, Zahra, Zahra Majd Taheri, Zahra Tanha Maafi, Zahra Majd Taheri, Zahra Tanha Maafi, Sergei A. Subbotin, Zahra Majd Taheri, et al. "Molecular and phylogenetic studies on Pratylenchidae from Iran with additional data on Pratylenchus delattrei, Pratylenchoides alkani and two unknown species of Hirschmanniella and Pratylenchus." Nematology 15, no. 6 (2013): 633–51. http://dx.doi.org/10.1163/15685411-00002707.

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Thirteen species of Pratylenchidae: Pratylenchus coffeae, P. delattrei, P. loosi, P. neglectus, P. penetrans, P. pseudopratensis, P. thornei, P. vulnus, Pratylenchus sp., Pratylenchoides alkani, P. ritteri, Hirschmanniella sp. and Zygotylenchus guevarai were collected from different crops and plants throughout Iran. The specimens were identified using morphological and molecular methods. Morphometrics and morphology are given for Pratylenchus sp., P. delattrei, Pratylenchoides alkani and Hirschmanniella sp. The D2-D3 expansion segments of the 28S rRNA gene were amplified and sequenced for all 13 species studied. Diagnostic PCR-ITS-RFLP profiles are given for Pratylenchus delattrei, P. penetrans, P. pseudopratensis, Pratylenchus sp., Pratylenchoides alkani and P. ritteri. Pratylenchus neglectus and P. thornei, collected from cereal fields, P. loosi from tea plantations, P. coffeae from banana, P. penetrans from ornamental plants, P. vulnus from pines and Z. guevarai from almonds showed a high level of similarity in the D2-D3 sequences with corresponding GenBank sequences. Nucleotide differences between Iranian populations and reference species were in the intraspecific range. Pratylenchus delattrei, found in vegetable fields, and Pratylenchus sp. from palm rhizosphere, formed a highly supported clade with P. zeae, the two former species being morphologically very close to the latter except in tail shape. Pratylenchus pseudopratensis, from cereal fields, clustered with P. vulnus with low support. Phylogenetic relationships within Pratylenchus species were mainly congruent with those obtained in previous studies. Despite the morphological similarities between P. ritteri and P. alkani, the D2-D3 of 28S rRNA gene sequences differed by 5 bp. Hirschmanniella sp., from a rice field, formed a clade with H. loofi and H. kwazuna.
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3

Troccoli, Alberto, Elena Fanelli, Pablo Castillo, Gracia Liébanas, Alba Cotroneo, and Francesca De Luca. "Pratylenchus vovlasi sp. Nov. (Nematoda: Pratylenchidae) on Raspberries in North Italy with a Morphometrical and Molecular Characterization." Plants 10, no. 6 (May 26, 2021): 1068. http://dx.doi.org/10.3390/plants10061068.

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Root-lesion nematode species rank third only to root-knot and cyst nematodes as having the greatest economic impact on crops worldwide. A survey of plant-parasitic nematodes associated with decaying raspberries (Rubus sp.) in northern Italy revealed that root-lesion nematodes were the most frequently occurring species among other phytonematodes. Several Pratylenchus species have been associated with Rubus sp. in Canada (Quebec, British Columbia) and USA (North Carolina, Maryland, New Jersey) including P. penetrans and P. crenatus. In the roots and rhizosphere of symptomatic raspberries, nematodes of two Pratylenchus spp. were detected. Detailed morphometrics of the two root-lesion nematode isolates were consistent with Pratylenchus crenatus and with an undescribed Pratylenchus species. The extracted nematodes were observed and measured as live and fixed materials and subsequently identified by integrative taxonomy (morphometrically and molecularly). The latter species is described herein as Pratylenchus vovlasi sp. nov., resulting morphometrically closest to P. mediterraneus and phylogenetically to P. pratensis. The molecular identification of Pratylenchus vovlasi sp. nov. was carried out by sequencing the ITS region, D2-D3 expansion domains of the 28S rRNA gene and a partial region of the nuclear hsp90 gene. ITS-RFLP and sequence analyses revealed that Pratylenchus vovlasi sp. nov. had species-specific restriction profiles with no corresponding sequences present in the database. The phylogenetic relationships with ITS and D2-D3 sequences placed the Pratylenchus vovlasi sp. nov. in a clade with P. pratensis and P. pseudopratensis. This research confirms the occurrence of cryptic biodiversity within the genus Pratylenchus as well as the need for an integrative approach to the identification of Pratylenchus species.
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4

Handoo, Zafar A., Guiping Yan, Mihail R. Kantor, Danqiong Huang, Intiaz A. Chowdhury, Addison Plaisance, Gary R. Bauchan, and Joseph D. Mowery. "Morphological and Molecular Characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a New Root-Lesion Nematode Species on Soybean in North Dakota, USA." Plants 10, no. 1 (January 17, 2021): 168. http://dx.doi.org/10.3390/plants10010168.

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Root-lesion nematodes (Pratylenchus spp.) of the genus Pratylenchus Filipjev, 1936, are among the most important nematode pests on soybean (Glycine max (L.) Merr.), along with soybean cyst and root-knot nematodes. In May 2015 and 2016, a total of six soil samples were collected from a soybean field in Walcott, Richland County, ND and submitted to the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), USDA, ARS, MD for analysis. Later, in 2019, additional nematodes recovered from a greenhouse culture on soybean originally from the same field were submitted for further analysis. Males, females, and juveniles of Pratylenchus sp. were recovered from soil and root samples and were examined morphologically and molecularly. DNA from single nematodes were extracted, and the nucleotides feature of three genomic regions targeting on the D2–D3 region of 28S rDNA and ITS rDNA and mitochondrial cytochrome oxidase subunit I (COX1) gene were characterized. Phylogeny trees were constructed to ascertain the relationships with other Pratylenchus spp., and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to provide a rapid and reliable differentiation from other common Pratylenchus spp. Molecular features indicated that it is a new, unnamed Pratylenchus sp. that is different from morphologically closely related Pratylenchus spp., including P. convallariae, P. pratensis, P. fallax, and P. flakkensis. In conclusion, both morphological and molecular observations indicate that the North Dakota isolate on soybean represents a new root-lesion nematode species which is named and described herein as Pratylenchus dakotaensis n. sp.
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5

de la Peña, Eduardo, Maurice Moens, and Gerrit Karssen. "Distribution and diversity of root-lesion nematodes (Pratylenchus spp.) associated with Ammophila arenaria in coastal dunes of Western Europe." Nematology 9, no. 6 (2007): 881–901. http://dx.doi.org/10.1163/156854107782331289.

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AbstractThe distribution and diversity of Pratylenchus species associated with Ammophila arenaria was investigated in its natural range of distribution. Twelve localities with vigorous stands of A. arenaria along the European Atlantic and Mediterranean coasts were sampled. The populations were identified based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2D3 region. Pratylenchus spp. were present in all of the sampled sites. A total of 19 populations were detected belonging to Pratylenchus dunensis, P. brzeskii, P. pratensis or P. penetrans. Pratylenchus dunensis was widely distributed from Blakeney Point (UK) to Comporta (Portugal). Pratylenchus brzeskii was found in South European localities along the Atlantic coast and also in the Mediterranean region. Pratylenchus pratensis was found associated with A. arenaria for the first time and occurred at different locations along the Atlantic coast. Pratylenchus penetrans was only detected in Biarritz (France). The P. dunensis populations from the south west Iberian Peninsula differed from the original P. dunensis description and showed two incisures on the lip region instead of one. Pratylenchus brzeskii populations did not vary morphologically from the original descriptions; however, the range of their morphometrical characters was wider than that of the type population. The D2D3 rDNA region revealed large interspecific and low intraspecific variation, supporting the morphological identification. The phylogenetic relationships of the populations with respect to other species of the genus were inferred from partial sequences of the rDNA and positioned P. dunensis within the same group as P. convallariae, P. penetrans and P. fallax.
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6

Ryss, A. Y. "Genus Pratylenchus Filipjev: multientry and monoentry keys and diagnostic relationships (Nematoda: Tylenchida: Pratylenchidae)." Zoosystematica Rossica 10, no. 2 (April 22, 2002): 241–55. http://dx.doi.org/10.31610/zsr/2001.10.2.241.

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Tabular (multientry) key to Pratylenchus is presented, and functioning of the computerized multientry image-operating key developed on the basis of the stepwise computer diagnostic system BIKEY-PICKEY is described. Monoentry key to Pratylenchus is given, and diagnostic relationships are analysed with the routine taxonomic methods as well as with the use of BIKEY diagnostic system and by the cluster tree analysis using STATISTICA program package. The synonymy Pratylenchus scribneri Steiner in Sherbakoff & Stanley, 1943 = P. jordanensis Hashim, 1983, syn. n. is established. Conclusion on the transition from amphimixis to parthenogenesis as one of the leading evolutionary factors for Pratylenchus is drawn.
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7

Leiva, Nathalia Petronila F., Simone M. de Santana-Gomes, Monique Thiara R. e. Silva, André Vinícius Zabini, Luz Marina G. Velázquez, and Claudia Regina Dias-Arieira. "Correlations Between Pratylenchus and Meloidogyne Populations, Soil Chemical Properties, Soil Texture, and Nutritional Status of Soybean Crops in Paraguay." Journal of Agricultural Science 15, no. 5 (April 15, 2023): 78. http://dx.doi.org/10.5539/jas.v15n5p78.

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Nematodes cause great damage to soybean crops in Paraguay. Studies have investigated correlations between phytonematodes and soil chemical and physical properties, but little is known about correlations with the nutritional status of soybean crops. This study aimed to assess correlations between Pratylenchus, Meloidogyne, soil chemical properties, soil texture, and the nutritional status of soybean. The experiment was carried out in Paraguay in areas of commercial soybean cultivation infested with nematodes, totaling 83 collection sites. Analyses of nematodes in soil and root samples, chemical characterization of soil acidity, fertility, and texture, and chemical characterization of soybean leaves were performed, totaling 36 variables. Data were subjected to principal component analysis. Soil Al3+ favored the development of Pratylenchus populations. Organic carbon negatively influenced Meloidogyne. K+ and Mg2+ negatively affected Pratylenchus and Meloidogyne, respectively. Pratylenchus and Meloidogyne correlated negatively with clay contents. In sandy soils, there was a negative correlation between Pratylenchus and sand content. Pratylenchus and Meloidogyne led to an increase in foliar Ca and a decrease in foliar P. Soil fertility management can be used as part of the integrated management of Pratylenchus and Meloidogyne. It is worth mentioning that, in field studies, the complexity of biotic and abiotic factors in the crop system may contribute to diverging results, making it difficult to establish a single response pattern, especially when some factors affect others.
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8

Bucki, Patricia, Xue Qing, Pablo Castillo, Abraham Gamliel, Svetlana Dobrinin, Tamar Alon, and Sigal Braun Miyara. "The Genus Pratylenchus (Nematoda: Pratylenchidae) in Israel: From Taxonomy to Control Practices." Plants 9, no. 11 (November 2, 2020): 1475. http://dx.doi.org/10.3390/plants9111475.

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Due to Israel’s successful agricultural production and diverse climatic conditions, plant-parasitic nematodes are flourishing. The occurrence of new, previously unidentified species in Israel or of suggested new species worldwide is a consequence of the continuous withdrawal of efficient nematicides. Among plant-parasitic nematodes, migratory endoparasitic species of the genus Pratylenchus are widely distributed in vegetable and crop fields in Israel and are associated with major reductions in quality and yield. This review focuses on the occurrence, distribution, diagnosis, pathogenicity, and phylogeny of all Pratylenchus species recorded over the last few decades on different crops grown throughout Israel—covering early information from nematologists to recent reports involving the use of molecular phylogenetic methodologies. We explore the accepted distinction between Pratylenchus thornei and Pratylenchus mediterraneus isolated from Israel’s northern Negev region, and address the confusion concerning the findings related to these Pratylenchus species. Our recent sampling from the northern Negev revealed the occurrence of both P. thornei and P. mediterraneus on the basis of molecular identification, indicating P. mediterraneus as a sister species of P. thornei and their potential occurrence in a mixed infection. Finally, the efficiencies of common control measures taken to reduce Pratylenchus’ devastating damage in protected crops and field crops is discussed.
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9

Nguyen, Dang Minh Chanh, and Woo Jin Jung. "Nematicidal properties of crude extracts obtained from medicinal plants against root-lesion nematode Pratylenchus coffeae." Journal of Vietnamese Environment 6, no. 3 (November 5, 2014): 264–69. http://dx.doi.org/10.13141/jve.vol6.no3.pp264-269.

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To investigate nematicidal activity against the root-lesion nematode, Pratylenchus coffeae, the methanolic extracts were obtained from 5 medicinal plants in Vietnam. Methanol extracts of the 5 samples were screened for nematicidal activity against Pratylenchus coffeae in vitro. Of the plant extracts tested, a 5 mg/ml concentration of Terminalia nigrovenulosa extract showed the highest level (95.0%) of nematicidal activity against Pratylenchus coffeae at 9 h after treatment. The remainder of the plant extracts was followed by Cinnamomum camphora (66.7%), Jasminum suptriplinerve (30.8%), Premna integrifolia (6.7%), and Treptocaulon juventas (5.8%). A 2.0 mg/mlconcentration of T. nigrovenulosa resulted in 13.3, 42.5, 62.5 and 86.7% at 1, 3, 5 and 7 h after treatment, respectively against Pratylenchus coffeae. Concentration of 0.1, 0.5, 1.0 and 2.0 mg/ml of T. nigrovenulosa resulted in 26.7, 71.7, 78.3 and 86.7% effectiveness respectively, at 7 h after treatment. Also, at 3 days after exposure to T. nigrovenulosa, hatch was inhibited by 60.0, 45.8, 17.5 and 5.8% at 0.1, 0.5, 1.0 and 2.0 mg/ml, respectively. Hatch was inhibited by 65.0, 50.8, 21.7 and 6.7% at 0.1, 0.5, 1.0 and 2.0 mg/ml respectively, at 6 days after exposure to T. nigrovenulosa. The mortality rate and hatch inhibition increased with increasing incubation time for crude extract of T. nigrovenulosa.
 Trong nghiên cứu này, 5 cây thuốc (Cinnamomum camphora, Jasminum suptriplinerve, Premna integrifolia, Terminalia nigrovenulosa, Treptocaulon juventas) đã được sử dụng làm nguyên liệu chiết xuất bằng methanol để thử hoạt tính kháng tuyến trùng Pratylenchus coffeae. Kết quả cho thấy chiết xuất từ cây Terminalia nigrovenulosa biểu hiện hoạt tính kháng tuyến trùng cao nhất (95,0%) sau 9 giờ xử lý với nồng độ 5 mg/ml. Hoạt tính kháng tuyến trùng của các chất chiết xuất được biểu hiện theo thứ tự giảm dần lần lượt là C. camphora (66,7%), J. suptriplinerve (30,8%), P. integrifolia (6,7%) và T. juventas (5,8%). Với nồng độ 2 mg/ml của chất chiết xuất từ T. nigrovenulossa biểu hiện hoạt tính gây chết tuyến trùng Pratylenchuss coffeae là 13,3; 42,5; 62,5 và 86,7% sau 1, 3, 5 và 7 giờ xử lý theo thứ tự. Ở cùng thời điểm sau xử lý 7 giờ, các nồng độ 0,1; 0,5; 1,0 và 2,0 mg/ml chiết xuất của T. nigrovenulossa có hoạt tính gây chết tuyến trùng Pratylenchus coffeae lần lượt là 26,7; 71,7; 78,3 và 86,7%. Bên cạnh đó, chất chiết xuất của T. nigrovenulossa cũng cho thấy tỷ lệ ức chế trứng nở cao. Cụ thể là sau 3 ngày xử lý với chất chiết xuất của T. nigrovenulossa, tỷ lệ ức chế trứng không nở là 60,0; 45,8; 17,5 và 5,8% at 0,1; 0,5; 1,0 và 2,0 mg/ml theo thứ tự. Sau 6 ngày xử lý với chất chiết xuất của T. nigrovenulossa, tỷ lệ ức chế trứng nở là 65,0; 50,8; 21,7 và 6,7% tại nồng độ 0,1; 0,5; 1,0 và 2,0 mg/ml theo thứ tự. Tỷ lệ gây chết và tỷ lệ ức chế của chất chiết xuất phụ thuộc vào cả thời gian và nồng độ xử lý.
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10

Thiruchchelvan, N., S. Casonato, G. Mikunthan, L. M. Condron, R. Moukarzel, and M. Kularathna. "Prevalence and abundance of plant-parasitic nematodes associated with corn (Zea mays L.) in Anuradhapura, Sri Lanka  ." Journal of the National Science Foundation of Sri Lanka 52, no. 3 (October 10, 2024): 299–309. http://dx.doi.org/10.4038/jnsfsr.v52i3.11729.

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Plant parasitic nematodes (PPN) are important pests of corn (Zea mays L.) Globally, while corn production has recently expanded in Sri Lanka. The population abundance, distribution, and pathological effects of nematodes that adversely affect corn production in Sri Lanka are poorly understood. This study was conducted to identify the prevalence of major PPN genera, population abundance, and their reproduction associated with corn fields in the Anuradhapura district. Over 92% of the corn fields were positive for PPN from the fields sampled in February 2021. Major PPN genera identified were Pratylenchus (71.4%) Helicotylenchus (28.6%), Meloidogyne (21.4%), Criconemella (21.4%), and Hoplolaimus (35.7%). The mean population of the Pratylenchus was 2020 kg-1 of soil, which was above the threshold level (1000 Pratylenchus kg-1 of soil), while other genera were lower than the threshold. All genera, except Meloidogyne, were observed from the intensive sampling from the selected fields during the next cropping season from November 2021 (seedling stage) to February 2022 (harvesting stage). Pratylenchus abundance in all the fields was lower than the threshold at the seedling stage and it was 2 to 10 times higher during the harvesting stage, except in Kelenikawewa where the initial Pratylenchus population (1865 kg-1 of soil) was also above the threshold. According to our results, Pratylenchus have the potential to impact corn yield in Anuradhapura. Future field experiments will determine the impact of PPN on corn production in Sri Lanka.
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11

Puerari, Heriksen H., Claudia R. Dias-Arieira, Mara M. Moura, Fabio Biela, Fernando M. Chiamolera, and Tatiana P. L. da Cunha. "Reação de porta-enxertos de videira a Pratylenchus brachyurus e Pratylenchus zeae." Tropical Plant Pathology 37, no. 3 (June 2012): 220–22. http://dx.doi.org/10.1590/s1982-56762012000300010.

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12

Riley, I. T., and S. J. Kelly. "Endoparasitic nematodes in cropping soils of Western Australia." Australian Journal of Experimental Agriculture 42, no. 1 (2002): 49. http://dx.doi.org/10.1071/ea01054.

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Soil samples were collected in Western Australia from cereal fields at about harvest time to determine levels of the endoparasitic nematodes, Pratylenchus and Heterodera. A systematic survey in 1997 and 1998 centred on the 40 shires with the highest proportion of cereal cropping. One hundred and eighty samples were collected in 1997 and 227 in 1998 at 10-km intervals along north–south transects 35 km apart. A targeted survey in 1997 included 98 soil samples from fields selected by growers as having poor productivity without an evident cause. No Heterodera cysts were found in any survey sample but these could be extracted from soils previously known to be infested. Pratylenchus neglectus was most commonly detected followed by P. thornei and P. zeae. Populations identified as P. brachyurus, P. penetrans, P. scribneri, and an undescribed species similar to P. thornei were also found. Overall Pratylenchus was extracted from 63% of samples (mean 1.1, median 0.3, max. 22.0/mL of soil). Maps of the data indicate that Pratylenchus populations were aggregated with some areas having relatively light infestations. There was a positive relationship with the intensity of cereal cropping and a negative relationship with pulse cropping. The findings indicate that potentially damaging Pratylenchus numbers occur in a significant proportion of fields and highlight the need to develop and implement strategies to lower population densities.
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13

WingChing-Jones, Rodolfo, and Luis Salazar Figueroa. "Yearly nematode population variation in Stargrass, Tannergrass, Smutgrass and Setariagrass in Alajuela, Costa Rica." UNED Research Journal 7, no. 1 (June 5, 2015): 49–57. http://dx.doi.org/10.22458/urj.v7i1.861.

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Nematode populations can affect productivity in cattle grasslands.Every 30 days from January 2004 to January 2005 we measured nematodepopulations in grass rhizospheres in northeastern Costa Rica.The substrate, sampling moment, and their interaction, were significant factors in the populations (p<0,05). We found Pratylenchus sp.,Helicotylenchus sp., Tylenchus sp. and non-parasitic nematodes (1,02;2,36, 1,28 y 3,87 log10 N° nematodes per 100g of roots respectively)in Stargrass throughout the year. Criconemella sp., Hemicycliophorasp., Ditylenchus sp. and Xiphinema sp. were occasionally identified. In Smutgrass, Meloidogyne sp., Pratylenchus sp., Paratylenchus sp and non-parasitic nematodes were identified (2,56; 2,51; 3,47 y 3,53 log10 N° nematodes per 100g of roots respectively).The size of plant-arasitic nematode populations in the roots was surprisingly high. We also found Pratylenchus sp., Helicotylenchus sp. and non-parasitic nematodes in Setariagrass roots, with densities of 2,92, 2,02 and 3,57 log10 N° nematodes per 100g respectively. Finally, in Tannergrass, Pratylenchus sp., Helicotylenchus sp. and non-parasitic nematodes were present (3,99; 1,47 and 3,79 log10 N°. nematodes roots 100g respectively).
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Smiley, Richard W., Guiping Yan, and Jennifer A. Gourlie. "Selected Pacific Northwest Rangeland and Weed Plants as Hosts of Pratylenchus neglectus and P. thornei." Plant Disease 98, no. 10 (October 2014): 1333–40. http://dx.doi.org/10.1094/pdis-12-13-1295-re.

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Eighteen rangeland plants and 16 weed species were assayed in the greenhouse for efficiency as hosts of Pratylenchus neglectus and P. thornei. Hosting ability ratings were assigned using the ratio of final versus initial nematode density and by comparing the final nematode density to that of susceptible wheat controls. Good hosts of both Pratylenchus spp. included thickspike bluegrass ‘Critana’, smooth brome ‘Manchar’, seven wheatgrasses, and jointed goatgrass. Good hosts of P. neglectus but not P. thornei included two hairy vetches, western wheatgrass ‘Rosana’, big bluegrass ‘Sherman’, tall wheatgrass ‘Alkar’, green foxtail, kochia, large crabgrass, palmer amaranth, redroot pigweed, tumble mustard, and wild oat. Good hosts of P. thornei but not P. neglectus included hard fescue ‘Durar’, sheep fescue ‘Blacksheep’, downy brome, and rattail fescue. Poor or minor hosts of both Pratylenchus spp. included two alfalfas, dandelion, horseweed, lambsquarters, prostrate spurge, and Russian thistle. These assays will provide guidance for transitioning rangeland into crop production and for understanding the role of weeds on densities of Pratylenchus spp. in wheat-production systems.
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Smiley, Richard W., Jason G. Sheedy, and Sandra A. Easley. "Vertical Distribution of Pratylenchus spp. in Silt Loam Soil and Pacific Northwest Dryland Crops." Plant Disease 92, no. 12 (December 2008): 1662–68. http://dx.doi.org/10.1094/pdis-92-12-1662.

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Dryland field crops in the Pacific Northwest United States are commonly produced in silt loams infested by the root-lesion nematodes Pratylenchus neglectus and P. thornei. Soils at 30 sites in Oregon were sampled from 0 to 120 cm depth to examine the vertical distribution of these Pratylenchus spp. Both species were distributed through entire soil profiles of all cropping systems. Populations were generally greatest in the surface 30 cm, but sometimes high populations were detected at depths greater than 45 cm. Sampling to 30 cm depth allowed detection of more than 50% of the population in most sites, while sampling to 45 cm depth yielded more than 75% of the population in over 75% of the sites evaluated. Therefore, soil samples should be collected to 30 to 45 cm depth to accurately estimate populations of Pratylenchus spp. in dryland crops produced on silt loams in the Pacific Northwest. Populations of Pratylenchus spp. were found to be related to the most recently planted crop, with populations after barley, after wheat, and during summer fallow being detected in ascending order.
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Gray, Michael, Horacio Lopez-Nicora, Tesfamariam Mekete, Terry Niblack, and Kimberly Reynolds. "Distribution and diversity of root-lession nematode (Pratylenchus spp.) associated with Miscanthus × giganteus and Panicum virgatum used for biofuels, and species identification in a multiplex polymerase chain reaction." Nematology 13, no. 6 (2011): 673–86. http://dx.doi.org/10.1163/138855410x538153.

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AbstractThe distribution of Pratylenchus spp. from bioenergy field plots in six states (Illinois, Iowa, South Dakota, Kentucky, Tennessee, Georgia) of the USA were surveyed. The species were identified based on morphology and morphometrics and further characterised based on fragment sequences of the 28S rRNA of the D2-D3 region. The region revealed variations in sequencing information that supports the morphological identification. In this work, six Pratylenchus spp. were detected: Pratylenchus brachyurus, P. crenatus, P. hexincisus, P. neglectus, P. penetrans and P. scribneri. Pratylenchus scribneri, P. crenatus, and P. penetrans were distributed most widely, with detection of 34, 29 and 15%, respectively. Pratylenchus hexincisus, P. brachyurus and P. neglectus were distributed sporadically, with detection rates of 10.0, 2.6 and 2.0%, respectively. A one-step multiplex PCR was developed for the simultaneous detection of P. scribneri, P. crenatus and P. penetrans. Sequence data from this research and NCBI were used to generate different primer sets that are species-specific. We have therefore designed three sets of primers that discriminate P. scribneri, P. crenatus and P. penetrans in multiplex PCR. All the tested primers have shown specificity and have no cross-reaction with the non-target species. When used in a uniplex, duplex and triplex PCR, the selected three primers gave a unique electrophoretic DNA banding pattern characterised by a single DNA fragment for P. scribneri (ca 750), P. crenatus (ca 690), and P. penetrans (ca 520). The method could be used for routine diagnostic programmes.
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FATEMI, EHSAN, and HABIBALLAH CHAREHGANI. "Root Lesion Nematode Pratylenchus thornei." Plant Pathology Science 7, no. 1 (March 1, 2018): 28–39. http://dx.doi.org/10.29252/pps.7.1.28.

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18

Pereira, Amanda Casagrande, Luciana Claudia Toscano, Alexandra Botelho Abreu, Noemi Souza Vieira, and Pamella Mingotti Dias. "OCORRÊNCIA DE NEMATÓIDES FITOPARASITOS EM SOLO CULTIVADO COM ALGODÃO E SOJA." JOURNAL OF NEOTROPICAL AGRICULTURE 2, no. 4 (December 4, 2015): 14–19. http://dx.doi.org/10.32404/rean.v2i4.276.

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O objetivo da pesquisa foi identificar a dinâmica populacional das espécies de nematóides nas culturas de algodão e soja em Cassilândia-MS. O experimento foi instalado na Universidade Estadual de Mato Grosso do Sul (UEMS), em duas áreas com 500 m² para cada cultura. As coletas de solo e raiz foram realizadas a cada 15 dias desde o início até o término das culturas, totalizando oito coletas. As amostras coletadas foram encaminhadas para o laboratório da Fundação Chapadão, em Chapadão do Sul-MS. Os nematóides identificados nas culturas foram dos gêneros Pratylenchus, Meloidogyne, Heterodera, Helicotylenchus, Mesocriconema, sendo a maior população encontrada para o gênero Pratylenchus, variando de 10 a 100 indivíduos/100 cm3 de solo para nos cultivos de soja e algodão. Quanto à ocorrência nas raízes houve variação de 560 a 5100 indivíduos/10 g para a soja, e de 70 a 2400/10 g para algodão. Os nematóides encontrados nas culturas de algodão e soja foram: Pratylenchus sp., P. zeae, P. brachyurus e Heterodera sp. O gênero Helicotylenchus sp. infectou somente o algodão e Meloidogyne sp. e Mesocriconema sp. exclusivamente a cultura da soja.Palavras-chave: Fitonematóides, Helicotylenchus, Pratylenchus, Heterodera, Meloidogy
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Cepeda Siller, Melchor, Jesús García Camargo, and Martín A. Alonso Gómez. "Identificación y dinámica poblacional de Pratylenchus spp. en manzano (Pyrus malus L.) en Arteaga, Coahuila." Revista Agraria 7, no. 2 (December 1, 1991): 174–83. http://dx.doi.org/10.59741/agraria.v7i2.94.

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Dentro de los principales problemas parasitológicos que se presentan en el cultivo del manzano en las regiones productoras del mundo, se encuentra el nematodo de la lesión Pratylenchus spp., por lo que el presente trabajo tiene como objetivos principales el identificar las especies de Pratylenchus asociadas a la rizófera del manzano en dos huertas (riego y temporal) ubicadas en el cañón de la Carbonera, Municipio de Arteaga, Coahuila; y a la vez, determinar la dinámica poblacional del nematodo y el número de generaciones presentes en el año en las mencionadas huertas. Bajo las condiciones en las que se desarrolló el trabajo, se definió la presencia de Pratylenchus penetrans y Pratylenchus brachyurus, determinándose esta última especie como la más importan- te para las huertas mencionadas. La población presente en muestras de suelo de la huerta de riego presentó dos generaciones en mayo y septiembre y en el sistema radical en los meses de mayo, julio y agosto; en la huerta de temporal se encontraron tres generaciones en los meses de mayo, agosto y octubre y en el sistema radical en los meses de mayo, julio y septiembre.
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20

Tanimola, A. A., O. Olotu, and A. O. Asimiea. "Occurrence, Diversity and Abundance of Nematode Pests of Pineapple (Ananas comosus) in Two Local Government Areas of Rivers State, Nigeria." Journal of Applied Sciences and Environmental Management 25, no. 4 (October 11, 2021): 665–75. http://dx.doi.org/10.4314/jasem.v25i4.29.

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Plant-parasitic nematodes (PPNs) contribute to yield losses in pineapple production. Limited studies have been conducted on plant-parasitic nematodes on pineapple despite reports of PPNs infecting it. A survey was conducted in Obio-Akpor and Ikwerre Local Government Areas (LGAs) of Rivers State to determine the occurrence, abundance and diversity of PPNs associated with pineapple. A total of 60 composite samples were collected from the two LGAs. Nematodes were extracted from soil and root samples using the modified Baermann technique and identified using standard procedures. Data were processed using relative frequency of occurrence (RFOC), relative importance values (RIVs), diversity indices, nematode population was calculated and analysis of variance. The most occurring plant-parasitic nematodes were Pratylenchus, Helicotylenchus, Meloidogyne, Scutellonema and Tylenchulus. In Obio Akpor LGA, Helicotylenchus with RFOC 33.3% was the most occurring nematode pests, while Pratylenchus was the most important plant-parasitic nematode (RIV 33.8%). In Ikwerre LGA, both Helicotylenchus and Pratylenchus have the same occurrence (RFOC 33.3%) with Pratylenchus being the most important nematode pest. Pratylenchus species with RIV of 52.4% were the most important PPNs associated with pineapple in both Obio-Akpor and Ikwerre LGAs. Dominance and Shannon- Weiner indices among the PPNs are 0.58 and 0.86, respectively. In Ikwerre and Obio-Akpor LGAs, Aluu (1530) and Rumuekini (630) pineapple farms have the highest population of PPNs, respectively. Ikwerre LGA (2,150) pineapple farms had more PPNs than Obio-Akpor LGA (660). Nematode pests are associated with pineapple in the two LGAs and could be contributing to yield losses.
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21

Tanimola, A. A., O. Olotu, and A. O. Asimiea. "Occurrence, Diversity and Abundance of Nematode Pests of Pineapple (Ananas comosus) in Two Local Government Areas of Rivers State, Nigeria." Journal of Applied Sciences and Environmental Management 25, no. 4 (October 11, 2021): 665–75. http://dx.doi.org/10.4314/jasem.v25i4.29.

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Plant-parasitic nematodes (PPNs) contribute to yield losses in pineapple production. Limited studies have been conducted on plant-parasitic nematodes on pineapple despite reports of PPNs infecting it. A survey was conducted in Obio-Akpor and Ikwerre Local Government Areas (LGAs) of Rivers State to determine the occurrence, abundance and diversity of PPNs associated with pineapple. A total of 60 composite samples were collected from the two LGAs. Nematodes were extracted from soil and root samples using the modified Baermann technique and identified using standard procedures. Data were processed using relative frequency of occurrence (RFOC), relative importance values (RIVs), diversity indices, nematode population was calculated and analysis of variance. The most occurring plant-parasitic nematodes were Pratylenchus, Helicotylenchus, Meloidogyne, Scutellonema and Tylenchulus. In Obio Akpor LGA, Helicotylenchus with RFOC 33.3% was the most occurring nematode pests, while Pratylenchus was the most important plant-parasitic nematode (RIV 33.8%). In Ikwerre LGA, both Helicotylenchus and Pratylenchus have the same occurrence (RFOC 33.3%) with Pratylenchus being the most important nematode pest. Pratylenchus species with RIV of 52.4% were the most important PPNs associated with pineapple in both Obio-Akpor and Ikwerre LGAs. Dominance and Shannon- Weiner indices among the PPNs are 0.58 and 0.86, respectively. In Ikwerre and Obio-Akpor LGAs, Aluu (1530) and Rumuekini (630) pineapple farms have the highest population of PPNs, respectively. Ikwerre LGA (2,150) pineapple farms had more PPNs than Obio-Akpor LGA (660). Nematode pests are associated with pineapple in the two LGAs and could be contributing to yield losses.
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22

Swibawa, I. Gede, Irma Amaliah, and Titik Nur Aeny. "PENGARUH INFESTASI NEMATODA Pratylenchus TERHADAP PERTUMBUHAN TANAMAN NENAS [Ananas comosus (L.) Merr.]." Jurnal Hama dan Penyakit Tumbuhan Tropika 1, no. 1 (March 2, 2001): 25–28. http://dx.doi.org/10.23960/j.hptt.1125-28.

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Pratylenchus is an important parasitic nematode on pineapples in the world. This plant parasitic nematodes may cause severe damages on roots, resulted in retarded plant growth. The experiment consisted of four Pratylenchus population levels per plant with 4 replications. The experiment was conducted on June to September 1999, on PT. Great Giant Pineapple Company (GGPC) in Central Lampung District. The aim of the research was to study the nematode population effects on root damages and plant growth. The result showed that smooth cayenne variety was a favorable host of Pratylenchus. Rapid rate of nematode population occurred in three months. The root damage of infested plant with as much as 100 - 300 nematodes per plant was up to 20.39 - 31.72 percent and caused canopy wet weight was a half lower than those untreated plant.
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23

Blair, B. L., G. R. Stirling, and P. J. L. Whittle. "Distribution of pest nematodes on sugarcane in south Queensland and relationship to soil texture, cultivar, crop age and region." Australian Journal of Experimental Agriculture 39, no. 1 (1999): 43. http://dx.doi.org/10.1071/ea98085.

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Summary. Five plant–parasitic nematode species were found to be widespread on sugarcane crops surveyed in south Queensland, namely Pratylenchus zeae, Meloidogyne javanica, Paratrichodorus minor, Helicotylenchus dihystera and Tylenchorhynchus annulatus. Apart from Meloidogyne, high nematode populations were found in most soil types, suggesting more extensive crop losses could be occurring than previously estimated. The most important pests were P. zeae and M. javanica, as they were often found at high densities and their pathogenicity on sugarcane is established. Mean densities for most nematode genera did not differ significantly between sugarcane cultivars, except that fewer Pratylenchus were associated with cultivar CP51-21 than other cultivars surveyed. The density of Pratylenchus in roots was significantly higher in plant crops than in ratoon crops, whereas the density of Paratrichodorus was highest in first and second ratoons.
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24

Oliveira, Kethelin Cristine Laurindo de, Dejânia Vieira de Araújo, Tainara Porcher, João Paulo Ascari, Danielle Storck-Tonon, Cinthia Ramona Jiraneck da Rosa, Renan da Costa Vieira, Matheus Peixoto de Lima, Sumaya Ferreira Guedes, and Domingos de Jesus Rodrigues. "Distribuição populacional do nematoide das lesões radiculares no estado de Mato Grosso, Brasil." Research, Society and Development 11, no. 17 (December 19, 2022): e25111738203. http://dx.doi.org/10.33448/rsd-v11i17.38203.

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O nematoide das lesões radiculares tem causado danos econômicos crescentes nas culturas de explorações agropecuárias no Brasil, especialmente na região Centro-Oeste. Objetivou-se neste trabalho conhecer a distribuição do nematoide das lesões radiculares, Pratylenchus spp., no Estado de Mato Grosso, possibilitando conhecer as localidades com maior concentração deste nematoide ao longo do tempo. Através de dados obtidos de laudos emitidos no período de 2006 a 2015 foram realizadas análises de abundância, frequência de ocorrência, autocorrelação e confeccionados mapas da densidade populacional de Pratylenchus spp. no Estado. Também foram analisados dados anuais de temperatura média, pluviosidade anual, produtividade e área plantada no Estado no período. O gênero Pratylenchus está presente em 85% das amostras, apresentando perante a outros gêneros fitopatogênicos uma abundância de 76% de incidência. Os municípios de Sapezal (12%), Sorriso (9%), Diamantino (8%), Campo Novo do Parecis (8%), Primavera do leste (6%), Sinop (5%), Nova Mutum (4%) e Querência (4%) foram os que apresentaram maior abundancia de Pratylenchus spp., indicando alto nível de infestação do patógeno na região central do Estado de Mato Grosso, sendo o ano de 2011 o que apresentou maior infestação. Observou-se que houve aumento na área plantada, porém a produtividade se manteve próxima a média de 3,405 Kg ha-1, anos com pluviosidade acima de 1100 mm apresentaram aumento populacional do nematoide, a temperatura média de 25°C demonstrou ser ideal para seu desenvolvimento, demonstrando que o Pratylenchus spp. possui um ambiente favorável no Estado de Mato Grosso para seu aumento populacional, devido as condições climáticas serem propicias, tendo o produtor que utilizar táticas de manejo para o seu controle para minimizar perdas em suas lavouras.
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25

Carvalho, Rezanio Martins, Andrea Chaves Fiuza Porto, Elvira Maria Regis Pedrosa, Thais Fernanda da Silva Vicente, Luana Maria Alves Da Silva, Risoneide de Cássia Zeferino Silva, Emanuel Feitosa de Assunção, and Lilian Margarete Paes Guimarães. "Flutuação populacional de Meloidogyne e Pratylenchus em campos cultivados com diferentes variedades de cana-de-açúcar em áreas de estados do nordeste do Brasil." DELOS: DESARROLLO LOCAL SOSTENIBLE 16, no. 44 (July 3, 2023): 1203–20. http://dx.doi.org/10.55905/rdelosv16.n44-013.

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A cana-de-açúcar é uma das principais fontes de açúcar, álcool, aguardente além de ser usada para geração de eletricidade e também como ração animal. Dentre os patógenos que parasitam a cultura se destacam os nematoides. Para se adotar medidas eficazes no manejo dos fitonematoides é imprescindível considerar não apenas a espécie do patógeno, mas também o quanto deste está presente na área. Os levantamentos populacionais são importantes para identificação da comunidade nematológica presente na área, e determinação da distribuição desses patógenos numa determinada localidade. O objetivo do trabalho foi avaliar a flutuação populacional de fitonematoides ao longo de nove anos de manejo da cultura em áreas do Rio Grande do Norte, Alagoas e Pernambuco com o cultivo das seguintes variedades de cana-de-açúcar RB813804, RB867515, RB92579, SP791011 e SP813250 entre os anos de 2009 a 2018. Foi observado que as populações de Pratylenchus e Meloidogyne aumentou ao decorrer dos anos avaliados, sendo que nos anos de 2017 verificou-se as maiores populações para Pratylenchus e 2009 para Meloidogyne registrando-se a ocorrência de Meloidogyne e Pratylenchus em todas as variedades avaliadas, as maiores densidades do nematoide das galhas foi observada nas variedades RB813804, e SP791011 e menores nas variedades RB867515 RB92579. Com relação a Pratylenchus observou-se que RB867515, SP813250, apresentaram as maiores densidades de Pratylenchus enquanto que a SP791011 apresentou as menores densidades populacionais. No decorrer dos anos avaliados as populações dos nematoides aumentaram, isso se deve principalmente o fato das variedades cultivadas serem suscetíveis; outro fator que contribuiu foram os solos de textura arenosa que predominam nas usinas avaliadas facilitando o aumento da densidade populacional dos nematoides.
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26

Cui, Jiang-Kuan, Wen-Kun Huang, Huan Peng, Yan Lv, Ling-An Kong, Hui-Xia Li, Shu-Jie Luo, Yan Wang, and De-Liang Peng. "Efficacy Evaluation of Seed-Coating Compounds Against Cereal Cyst Nematodes and Root Lesion Nematodes on Wheat." Plant Disease 101, no. 3 (March 2017): 428–33. http://dx.doi.org/10.1094/pdis-06-16-0862-re.

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Cereal cyst nematodes (Heterodera avenae and H. filipjevi) and root lesion nematodes (Pratylenchus spp.) have been found to infect cereals in 16 provinces of China. To develop a nematicide that effectively controls nematodes, two novel chemical products, methylene bis thiocyanate (MBT) and MBT + thiamethoxam (MTT); four common pesticides, fipronil + chlorpyrifos (FIC), emamectin benzoate, imidacloprid, and Bacillus thuringiensis; and one fungicide, iprodione, were tested as seed coatings for the control of cereal cysts and root lesion nematodes from 2013 to 2015. Wheat seeds were treated with these seven seed coatings before sowing, and changes in the numbers of Heterodera spp. and Pratylenchus spp. were recorded during three different growth stages. Wheat yields were also compared after harvest. All treatments reduced the numbers of Pratylenchus in wheat and of cysts and eggs of Heterodera in the soil compared with the untreated control. Among the treatments, application of MTT or FIC was more effective than that of the other treatments for nematode control, and the other treatments had similar effects. The results of this study have demonstrated that MTT and FIC applied as seed treatments effectively reduce the number of cysts, inhibit the reproduction of Heterodera and Pratylenchus, and enhance wheat yields. MTT and FIC are thus suitable for controlling nematodes on wheat under natural field conditions.
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Nguyễn, Gia Huy, Thị Thu Nga Nguyễn, Vũ Phến Trần та Văn Hòa Nguyễn. "Nghiên cứu thành phần tuyến trùng ký sinh thực vật trên cây đậu phộng (Arachis hypogaea) tại tỉnh Trà Vinh". CTU Journal of Science 60, № 4 (30 серпня 2024): 157–66. http://dx.doi.org/10.22144/ctujos.2024.403.

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Thành phần tuyến trùng ký sinh thực vật (PPNs) liên quan đến cây đậu phộng được thực hiện tại các huyện Duyên Hải, Cầu Ngang và Trà Cú, tỉnh Trà Vinh. Phương pháp nghiên cứu dựa vào đặc điểm hình thái học và các chỉ số đo về hình thái lượng kết hợp một số chỉ số đánh giá đặc điểm sinh học quần xã trong đất. Kết quả khảo sát đã ghi nhận được 17 loài, 10 giống PPNs thuộc 8 họ bao gồm: Pratylenchus coffeae, P. brachyurus, Ditylenchus ausafi, Helicotylenchus crenacauda, Hirschmanniella oryzae, H. mucronata, Mesocriconema curvatum, M. ornatum, Mesocriconema sp., Meloidogyne graminicola, Tylenchorhynchus leviterminalis, T. mashhoodi, T. nudus, T. annulatus, Tylenchus sp., Longidorus elongatus và Xiphinema elongatum. Trong đó, tần suất hiện diện của giống Mesocriconema phổ biến trên nhóm đất cát chiếm 100% và đã ghi nhận sự xuất hiện của 3 giống Pratylenchus, Tylenchorhynchus và Hirschmanniella trong rễ cây đậu phộng; đồng thời đã ghi nhận sự hiện diện của giống tuyến trùng Pratylenchus phổ biến trong trái đậu phộng có triệu chứng đốm đen. Hai giống Mesocriconema và Pratylenchus tìm thấy trong nghiên cứu này là tác nhân ký sinh quan trọng trên cây đậu phộng và cần tiến hành các biện pháp phòng trừ.
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Olthof, Th H. A., and M. S. Wolynetz. "Pratylenchus penetrans and P. neglectus in tubers of potato (Solanum tuberosum) in Ontario." Canadian Journal of Plant Science 71, no. 4 (October 1, 1991): 1251–56. http://dx.doi.org/10.4141/cjps91-173.

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Root-lesion nematodes (Pratylenchus penetrans) were extracted from peels of potato tubers (Solanum tuberosum 'Russet Burbank'). The average number of P. penetrans recovered over a 2-wk period in a mist chamber from four batches of tubers, stored for 3–18 wk at 7 °C in a cold room, ranged from 11 to 1925 nematodes tuber−1. An average of 374 Pratylenchus neglectus tuber−1 were recovered from the peels of potato Norchip, grown in soil containing on average 4370 nematodes kg−1 of soil at harvest, and stored for 19 wk at 7 °C. P. penetrans and P. neglectus were recovered only from the outer layer of potato tubers; none was found in the inner core. Planting halves of tubers containing an average of 1925 P. penetrans tuber−1 into nematode-free soil resulted in soil population densities of 2225 P. penetrans kg−1 of soil and root population densities of 884 nematodes root system−1 after 8 wk in the greenhouse. Although many, if not most, potato fields in southern Ontario are already infested by P. penetrans, these studies have shown that stored, infested tubers can initiate a new infestation. Key words: Peel, potato tuber, Pratylenchus neglectus, Pratylenchus penetrans, root-lesion nematode, Solanum tuberosum
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29

B, Avhad Sunil, and Hiware Chandrashekhar J. "Studies on Population Dynamics of Pratylenchus sp. (Filipjev, 1936) about Soil Abiotic Factor in the Mulberry Field at Aurangabad, Maharashtra, India." Shanlax International Journal of Arts, Science and Humanities 8, S1-Feb (February 6, 2021): 67–72. http://dx.doi.org/10.34293/sijash.v8is1-feb.3933.

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In the present study, the monthly population fluctuation of Pratylenchus sp. (Filipjev, 1936) is ascertain about soil temperature, moisture, pH in mulberry (Morus alba L.) field with economic importance within the sericulture. The studies target is to grasp the influence and impact of those soil abiotic factors on the population of those plant-parasitic nematodes and Correlation coefficients (r) between mean population Pratylenchus spand different soil abiotic factors in Aurangabad Mulberry garden.
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30

Waeyenberge, Lieven, Alexander Ryss, Maurice Moens, Jorge Pinochet, and Thierry Vrain. "Molecular characterisation of 18 Pratylenchus species using rDNA Restriction Fragment Length Polymorphism." Nematology 2, no. 2 (2000): 135–42. http://dx.doi.org/10.1163/156854100509024.

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AbstractThe RFLP technique was used to establish a reliable diagnostic method for 18 Pratylenchus species: Pratylenchus agilis, P. bolivianus, P. brachyurus, P. coffeae, P. crenatus, P. fallax, P. goodeyi, P. loosi, P. mediterraneus, P. neglectus, P. penetrans, P. pratensis, P. pseudocoffeae, P. scribneri, P. subranjani, P. thornei, P. vulnus and P. zeae. The polymerase chain reaction (PCR) amplified the ITS regions from all species and populations examined and revealed large differences in length, ranging in size from approximately 900 to 1250 bp. The rDNA fragments were digested with five restriction enzymes (CfoI, DdeI, HindIII, HpaII, and PstI). All Pratylenchus species can be differentiated from each other by a combination of at least two enzymes. CfoI differentiated all nematode species with the exception of P. fallax, P. penetrans and P. pseudocoffeae. P. fallax was further separated by a DdeI restriction, and P. pseudocoffeae by a PstI digestion. Intraspecific RFLP were observed. Upon CfoI, DdeI, HindIII, or HpaII digestion, it was possible to separate the three P. coffeae populations studied from each other. La technique RFLP a été utilisée pour créer une méthode fiable de diagnostic pour 18 espèces de Pratylenchus: Pratylenchus agilis, P. bolivianus, P. brachyurus, P. coffeae, P. crenatus, P. fallax, P. goodeyi, P. loosi, P. mediterraneus, P. neglectus, P. penetrans, P. pratensis, P. pseudocoffeae, P. scribneri, P. subranjani, P. thornei, P. vulnus et P. zeae. La réaction de polymérisation en chaîne (PCR) a amplifié les régions de l’ITS pour toutes les espèces et populations étudiées et a mis en évidence de grandes différences dans la taille des gammes de longueur, de 900 à 1250 bp approximativement. Les fragments de rDNA ont été digérés à l’aide de cinq enzymes de restriction (CfoI, DdeI, HindIII, HpaII, and PstI). Toutes les espèces de Pratylenchus ont pu être différenciées les unes des autres par une combinaison d’au moins deux enzymes. CfoI a différencié toutes les espèces à l’exception de P. fallax, P. penetrans et P. pseudocoffeae. P. fallax a été ultérieurement séparé par une restriction DdeI, et P. pseudocoffeae par une digestion PstI. Des RFLP intraspécifiques ont été observés. Par les digestions CfoI, DdeI, HindIII, ou HpaII, il s’est révélé possible de séparer les unes des autres les trois populations étudiées de P. coffeae.
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Mekete, Tesfamariam, Kimberly Reynolds, Horacio D. Lopez-Nicora, Michael E. Gray, and Terry L. Niblack. "Plant-Parasitic Nematodes Are Potential Pathogens of Miscanthus × giganteus and Panicum virgatum Used for Biofuels." Plant Disease 95, no. 4 (April 2011): 413–18. http://dx.doi.org/10.1094/pdis-05-10-0335.

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A survey of Miscanthus × giganteus and switchgrass plots throughout the midwestern and southeastern United States was conducted to determine the occurrence and distribution of plant-parasitic nematodes associated with these biofuel crops. During 2008, rhizosphere soil samples were collected from 24 Miscanthus × giganteus and 38 switchgrass plots in South Dakota, Iowa, and Illinois. Additional samples were collected from 11 Miscanthus × giganteus and 10 switchgrass plots in Illinois, Kentucky, Georgia, and Tennessee the following year. The 11 dominant genera recovered from the samples were Pratylenchus, Helicotylenchus, Xiphinema, Longidorus, Heterodera, Hoplolaimus, Tylenchorhynchus, Criconemella, Paratrichodorus, Hemicriconemoides, and Paratylenchus. Populations of Helicotylenchus, Xiphinema, and Pratylenchus were common and recorded in 90.5, 83.8, and 91.9% of the soil samples from Miscanthus × giganteus, respectively, and in 91.6, 75, and 83.3% of the soil samples from switchgrass, respectively. Prominence value (PV) (PV = population density × √frequency of occurrence/10) was calculated for the nematodes identified. Helicotylenchus had the highest PV (PV = 384) and was followed by Xiphinema (PV = 152) and Pratylenchus (PV = 72). Several of the nematode species associated with the two biofuels crops were plant parasites. Of these, Pratylenchus penetrans, P. scribneri, P. crenatus, Helicotylenchus pseudorobustus, Hoplolaimus galeatus, X. americanum, and X. rivesi are potentially the most damaging pests to Miscanthus × giganteus and switchgrass. Due to a lack of information, the damaging population thresholds of plant-parasitic nematodes to Miscanthus × giganteus and switchgrass are currently unknown. However, damage threshold value ranges have been reported for other monocotyledon hosts. If these damage threshold value ranges are any indication of the population densities required to impact Miscanthus × giganteus and switchgrass, then every state surveyed has potential for yield losses due to plant-parasitic nematodes. Specifically, Helicotylenchus, Xiphinema, Pratylenchus, Hoplolaimus, Tylenchorhynchus, Criconemella, and Longidorus spp. were all found to have population densities within or above the threshold value ranges reported for other monocotyledon hosts.
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Navas-Cortés, Juan, Blanca Landa, Nicola Vovlas, Gracia Liébanas, Pablo Castillo, Juan Palomares-Rius, and Sergei Subbotin. "Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae." Nematology 12, no. 3 (2010): 429–51. http://dx.doi.org/10.1163/138855409x12559479585043.

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AbstractA new amphimictic species, Pratylenchus hispaniensis n. sp., parasitising the roots of gum cistus in Andújar (Jaén), southern Spain, is described. The new species is characterised by the presence of numerous males and by the female having a lip region with three annuli, a divided face, a robust stylet (14.5-17.0 μm) with rounded knobs, lateral fields with four lines, V = 80-84, a round spermatheca full of sperm, well developed post-vulval uterine sac and an obliquely truncate tail with irregularly annulated terminus. Morphologically this species is related to P. bhatti, P. kralli, P. mediterraneus, P. pseudofallax and P. thornei. A phenetic study of the 25 most useful diagnostic morphological and allometric characters for Pratylenchus species was done using multivariate factor and linear discriminant analyses. In the factor analysis the first seven factors accounted for 71.1% of the total variance of the characters selected. These factors were related to female tail, pharyngeal overlap, reproductive behaviour, stylet length, L/post-vulval uterine sac ratio, body length and number of lip annuli. Discriminant analysis differentiated Pratylenchus spp. from the three valid species of Zygotylenchus. The results of the phylogenetic analysis based on sequences of the D2-D3 expansion regions of 28S, partial 18S and ITS rRNA genes confirmed the close relationship of P. hispaniensis n. sp. with P. mediterraneus and inferred molecular affinity with P. brzeskii, P. neglectus and P. thornei, in spite of variation in the position of P. hispaniensis n. sp. in the clades. Additional phylogenetic analyses based on the same sets of sequences for P. hispaniensis n. sp., Zygotylenchus guevarai and other Pratylenchidae indicated that Pratylenchus includes several paraphyletic lineages; however, likelihood tests did not reject monophyly of the genus. The inclusion of Pratylenchus, Zygotylenchus, Hirschmanniella, Nacobbus and Apratylenchus in Pratylenchidae was supported.
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Waweru, Beth Wangui, Njira Njira Pili, Wim M. L. Wesemael, Cecilia Wanjau, Misghina G. Teklu, and Godelieve Gheysen. "Population dynamics and damage threshold of Pratylenchus n. sp. and Meloidogyne javanica on finger millet." Nematology 25, no. 10 (October 5, 2023): 1157–68. http://dx.doi.org/10.1163/15685411-bja10283.

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Summary Finger millet (Eleusine coracana) is a staple crop for subsistence farmers and is primarily cultivated in arid and semi-arid regions. Nevertheless, there are limited data available on the occurrence of plant-parasitic nematodes that specifically target finger millet, and the damage arising from such infections. In this study, we investigated the population dynamics, damage threshold and tolerance of the lesion nematode, Pratylenchus n. sp., and the root-knot nematode, Meloidogyne javanica, on finger millet ‘P-224’. We used 11 initial population densities (Pi) of second-stage juveniles (J2): 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32 and 64 J2 (g soil)−1 for M. javanica and the same densities of mixed life-stages for Pratylenchus n. sp. The logistic growth model, Seinhorst yield loss and population dynamic models were fitted to the shoot height, plant biomass and final nematode population density. Based on the population dynamics model parameters, maximum multiplication rates and maximum population densities for each nematode species were estimated. The maximum multiplication rates (a) were 32.39 and 17.46, whilst the maximum population densities (M) were 18.83 nematodes (g soil)−1 and 19.78 J2 (g soil)−1 for Pratylenchus n. sp. and M. javanica respectively. The maximum height reached (C) was negatively affected by increasing Pi for both nematode genera, while the rate of growth (B) and the time to reach was not affected based on the logistic model. A tolerance limit (T) of 1.70 nematodes (g soil)−1 and 0.65 J2 (g soil)−1; relative minimum grain yield (m) of 0.23 and 0.40 for Pratylenchus n. sp. and M. javanica was found, respectively. The results indicate that ‘P-224’ is a good host for both Pratylenchus n. sp. and M. javanica, which significantly reduce yield. These findings can be used as a base to develop effective nematode management strategies for finger millet.
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Elhady, Ahmed, Torsten Thünen, Frank Höppner, Christiane Balko, Holger Heuer, and Johannes Hallmann. "Evaluation of soybean cultivars for their susceptibility to root-lesion nematodes under temperate conditions." Nematology 21, no. 5 (2019): 523–31. http://dx.doi.org/10.1163/15685411-00003232.

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Summary Soybean, Glycine max, is a relatively new crop in Europe gaining increasing interest for its potential to fix atmospheric nitrogen and thus increase sustainability of modern agricultural production systems. Under temperate conditions, root-lesion nematodes (Pratylenchus spp.) are one of the most prominent plant-parasitic nematode taxa of economic concern in soybean. Here, we investigated the susceptibility of soybean cultivars towards naturally occurring populations of Pratylenchus spp. and their effects on soybean yield. At the site Bundesallee the cultivars ‘Abelina’, ‘Primus’ and ‘Taifun3’ supported the highest infestation and multiplication of a mixed population of P. neglectus and P. crenatus, while ‘Sultana’ and ‘Solena’ were least susceptible. At the field site Groß Lüsewitz, ‘Primus’ and ‘Merlin’ cultivars were significantly more susceptible to P. neglectus than ‘Sultana’. Soybean yield was reduced by high initial densities of Pratylenchus spp. Results are expected to contribute to a better nematode management.
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Dias-Arieira, Cláudia Regina, Cleber Furlanetto, Simone de Melo Santana, Davi Antonio Oliveira Barizão, Regina Cássia Ferreira Ribeiro, and Heloísa Maria Formentini. "Fitonematoides associados a frutíferas na região Noroeste do Paraná, Brasil." Revista Brasileira de Fruticultura 32, no. 4 (November 26, 2010): 1064–71. http://dx.doi.org/10.1590/s0100-29452010005000119.

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Com o objetivo de estudar a ocorrência de nematoides fitoparasitos em frutíferas cultivadas na região noroeste do Paraná, realizou-se um levantamento, envolvendo 124 amostras de solo e raízes coletadas de 19 espécies de frutíferas, em 15 municípios, no período de dezembro/2007 a fevereiro/2009. As amostras foram submetidas a extrações e avaliadas sob microscópio óptico. Foram constatados nove diferentes gêneros de nematoides. Em citros, a espécie mais frequente e abundante foi Tylenchulus semipenetrans, sendo também recuperados das amostras os gêneros Meloidogyne, Pratylenchus, Helicotylenchus, Xiphinema, Trichodorus, Mesocriconema e Dolichodorus. Nas demais frutíferas, os gêneros observados foram Meloidogyne, Pratylenchus, Helicotylenchus e Hemicycliophora. A maior abundância de Pratylenchus brachyurus ocorreu em abacaxizeiro, Meloidogyne incognita em figueira e caquizeiro, e Helicotylenchus dihystera e H. multicinctus em bananeira. Os principais gêneros de fitonematoides foram constatados em aproximadamente 50% das amostras, podendo representar risco para fruteiras da região se não manejados adequadamente.
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JESUS, ALNIUSA MARIA DE, ELVIRA MARIA REGIS PEDROSA, JOÃO BATISTA RIBEIRO DA SILVA REIS, and ANDRÉA CHAVES FIUZA PORTO. "MORPHOLOGICAL AND MORPHOMETRIC CHARACTERIZATION OF PRATYLENCHUS SPECIES IN SUGARCANE CROPPING AREAS OF PERNAMBUCO STATE." Revista Caatinga 33, no. 3 (September 2020): 599–607. http://dx.doi.org/10.1590/1983-21252020v33n303rc.

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ABSTRACT The root-lesion nematodes Pratylenchus spp. have a wide geographic distribution and a large number of host plants. These migratory endoparasites are found associated with sugarcane around the world causing diseases that induce permanent losses in productivity. Although this genus has ordinarily been reported in low-producing sugarcane areas in northeastern Brazil, studies of the species occurrence are few and restricted to a very low number of sites and samples. This study aimed to morphologically and morphometrically characterize Pratylenchus species from different sugarcane regions of Pernambuco state, where composite samples of soil and roots were collected on 30 farms from three sugarcane mills. The external morphological characters of the nematode body from different populations had a diagnostic value compatible with those obtained in the literature, showing the dominance of P. zeae (87%) in the region. Atypical populations of the genus Pratylenchus were also found.
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Kawanobe, Masanori, Soh Sugihara, Naoko Miyamaru, Koichi Yoshida, Eito Nonomura, Hiroaki Oshiro, and Koki Toyota. "Distribution of Root-Lesion and Stunt Nematodes, and Their Relationship with Soil Properties and Nematode Fauna in Sugarcane Fields in Okinawa, Japan." Agronomy 10, no. 6 (May 27, 2020): 762. http://dx.doi.org/10.3390/agronomy10060762.

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Sugarcane cultivation in Japan has not yet focused on suppressing plant-parasitic nematodes. For proper nematode management, it is essential to know the spatial distribution of economically important plant-parasitic nematodes and free-living nematodes that play important roles in terrestrial ecosystems. We aimed to reveal nematode fauna and soil properties in 85 sugarcane fields of three major sugarcane producing islands in Japan, and to examine their relationship by using the mixed-effect model and by visualizing the spatial distributions using the inverse distance weighting (IDW) approach. The nematode community structures were analyzed by non-metric multidimensional scaling (NMDS). Among plant-parasitic nematodes in sugarcane, the root-lesion nematodes (Pratylenchus sp.) and the stunt nematodes (Tylenchorhynchus sp.) were widely distributed in these islands, yet the abundance and the species varied geospatially. Soil pH was significantly correlated with the abundance of Pratylenchus and Tylenchorhynchus species. The abundance of Pratylenchus and Tylenchorhynchus species were significantly correlated with soil pH. The abundance of Pratylenchus was significantly correlated with the abundance of free-living nematodes, the number of free-living nematode species, and exchangeable cation K+, as were the abundance of Tylenchorhynchus to the clay content and that of non-Tylenchorhynchus. This study also revealed that the three islands had different nematode faunas, which were explained especially by soil pH, texture, and exchangeable basic cations.
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Smiley, Richard W., Stephen Machado, Jennifer A. Gourlie, Larry C. Pritchett, Guiping Yan, and Erling E. Jacobsen. "Effects of Crop Rotations and Tillage on Pratylenchus spp. in the Semiarid Pacific Northwest United States." Plant Disease 97, no. 4 (April 2013): 537–46. http://dx.doi.org/10.1094/pdis-08-12-0788-re.

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There is interest in converting rainfed cropping systems in the Pacific Northwest from a 2-year rotation of winter wheat and cultivated fallow to direct-seed (no-till) systems that include chemical fallow, spring cereals, and food legume and brassica crops. Little information is available regarding effects of these changes on plant-parasitic nematodes. Eight cropping systems in a low-precipitation region (<330 mm) were compared over 9 years. Each phase of each rotation occurred each year. The density of Pratylenchus spp. was greater in cultivated than chemical fallow, became greater with increasing frequency of host crops, and was inversely associated with precipitation (R2 = 0.92, α < 0.01). Densities after harvesting mustard, spring wheat, winter wheat, and winter pea were greater (α < 0.01) than after harvesting spring barley or spring pea. Camelina also produced low densities. Winter wheat led to a greater density of Pratylenchus neglectus and spring wheat led to a greater density of P. thornei. Density of Pratylenchus spp. was correlated (R2 = 0.88, α < 0.01) but generally higher when detected by real-time polymerase chain reaction on DNA extracts from soil than when detected by a traditional method. Selection of different Pratylenchus spp. by different wheat cultivars or growth habit must be addressed to minimize the level of nematode risk to future plantings of intolerant crops.
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Bahmani, Jebraeil, Farhad Khozeini, Shapour Barooti, Saeed Rezaee, and Reza Ghaderi. "PLANT-PARASITIC NEMATODES ASSOCIATED WITH WALNUT IN THE SANANDEJ REGION OF WEST IRAN." Journal of Plant Protection Research 53, no. 4 (October 1, 2013): 404–8. http://dx.doi.org/10.2478/jppr-2013-0060.

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Abstract A survey of plant-parasitic nematodes associated with walnut was carried out in the Sanandej region, of the province of Kurdistan, in western Iran, during the 2011-2012 time period. After taking samples from fifty-four localities and then doing the routine laboratory work for processing, fixing, and mounting of the nematodes, twenty-one species of plant-parasitic nematodes were finally identified. Among the identified species: Cacopaurus pestis, Mesocriconema xenoplax, Pratylenchus vulnus and Meloidogyne incognita are likely to cause damage. Spiral (Helicotylenchus crassatus, H. digonicus, H. pseudorobustus and H. vulgaris), ring (Mesocriconema antipolitanum and M. xenoplax) and root-lesion nematodes (Pratylenchus neglectus, P. thornei, P. vulnus and P. delattrei) were the most predominant nematodes in the sampled area (frequencies in soil were 87.0, 77.8, and 72.2% and 46.3, 20.4 and 14.8% in root samples, respectively). In the present study, Pratylenchus delattrei, Trophurus lomus, Paratylenchus similis, Geocenamus stegus, Helicotylenchus crassatus, Scutellonema brachyurus and Meloidogyne incognita were reported as new species associated with walnut in Iran.
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40

Mokrini, Fouad, Salah-Eddine Laasli, Youssef Karra, Aicha El Aissami, and Abdelfattah A. Dababat. "Diversity and incidence of plant-parasitic nematodes associated with saffron (Crocus sativus L.) in Morocco and their relationship with soil physicochemical properties." Nematology 22, no. 1 (September 4, 2019): 87–102. http://dx.doi.org/10.1163/15685411-00003286.

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Summary Saffron (Crocus sativus) fields in Morocco’s Taliouine and Taznakht regions were surveyed between January and April 2018 to study the diversity and incidence of plant-parasitic nematodes and assess the effects of soil physicochemical properties on the nematodes. Fourteen nematode genera were identified in soil and root samples collected from 66 saffron fields. The most common plant-parasitic nematodes in the Taliouine region were Pratylenchus spp. and Helicotylenchus spp. In the Taznakht region, the most common nematodes were Pratylenchus spp., Tylenchorhynchus spp. and Ditylenchus dipsaci. Nematodes, particularly Pratylenchus spp. and Ditylenchus spp., were abundant and frequent throughout the region. Several nematode genera were significantly associated with soil texture and mineral content, indicating that soil properties play an important role in plant-parasitic nematode communities. This description of plant-parasitic nematode assemblages associated with saffron fields in Morocco and their relationship with soil physicochemical properties provides a starting point from which appropriate nematode management strategies can be implemented.
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Smiley, Richard W., Guiping Yan, and Jennifer A. Gourlie. "Selected Pacific Northwest Crops as Hosts of Pratylenchus neglectus and P. thornei." Plant Disease 98, no. 10 (October 2014): 1341–48. http://dx.doi.org/10.1094/pdis-12-13-1296-re.

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Thirty crop species and cultivars were assayed in the greenhouse for efficiency as hosts of Pratylenchus neglectus and P. thornei. Hosting ability ratings were assigned using the ratio of final versus initial nematode density and also by comparing the final nematode density to that of a susceptible wheat control. Good hosts of both Pratylenchus spp. included oat ‘Monida’, chickpea ‘Myles’, and lentil ‘Athena’ and ‘Morton’. Good hosts of P. neglectus but not of P. thornei included 10 Brassica spp. (5 canola, 2 mustard, and 3 camelina cultivars), chickpea ‘Sierra’, sudangrass ‘Piper’, and sorghum/sudangrass hybrid ‘Greentreat Plus’. Good hosts of P. thornei but not of P. neglectus included lentil ‘Skyline’ and pea ‘Granger’, ‘Journey’, and ‘Universal’. Poor or minor hosts of both Pratylenchus spp. included chickpea ‘Dwelley’, pea ‘Badminton’, safflower ‘Gila’, ‘Girard’, and ‘KN 144’, sunflower ‘2PD08’, flax ‘Pembina’, eastern gamagrass ‘Pete’, and switchgrass ‘Blackwell’. Results of these assays will provide guidance for improving crop rotation and cultivar selection efficiencies.
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42

"Pratylenchus thornei." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.43903.

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This datasheet on Pratylenchus thornei covers Identity, Overview, Distribution, Dispersal, Hosts/Species Affected, Diagnosis, Biology & Ecology, Natural Enemies, Impacts, Prevention/Control, Further Information.
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43

"Pratylenchus pratensis." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.43901.

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44

"Pratylenchus crenatus." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.40277156.

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45

"Pratylenchus rwandae." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.94198679.

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46

"Pratylenchus hippeastri." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.96108117.

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47

"Pratylenchus scribneri." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.43902.

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48

"Pratylenchus capsici." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.62902238.

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49

"Pratylenchus bolivianus." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.116527.

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50

"Pratylenchus speijeri." CABI Compendium CABI Compendium (January 7, 2022). http://dx.doi.org/10.1079/cabicompendium.119481.

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