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1

Andersson, Daniel, David Svec, Cathrine Pedersen, Jørn Henriksen, and Anders Ståhlberg. "Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons." International Journal of Molecular Sciences 19, no. 10 (2018): 3185. http://dx.doi.org/10.3390/ijms19103185.

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Analyzing rare DNA and RNA molecules in limited sample sizes, such as liquid biopsies and single cells, often requires preamplification, which makes downstream analyses particularly sensitive to polymerase chain reaction (PCR) generated contamination. Herein, we assessed the feasibility of performing Cod uracil-DNA N-glycosylase (Cod UNG) treatment in combination with targeted preamplification, using deoxyuridine triphosphate (dUTP) to eliminate carry-over DNA. Cod UNG can be completely and irreversibly heat inactivated, a prerequisite in preamplification methods, where any loss of amplicons i
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2

Ridley, K. D., and A. M. Scott. "High-reflectivity phase conjugation using Brillouin preamplification." Optics Letters 15, no. 14 (1990): 777. http://dx.doi.org/10.1364/ol.15.000777.

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3

Elmirghani, J. M. H., and R. A. Cryan. "Jitter implications on PPM using optical preamplification." Electronics Letters 30, no. 1 (1994): 60–62. http://dx.doi.org/10.1049/el:19940032.

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4

Hsieh, Kuangwen, Guojie Zhao, and Tza-Huei Wang. "Applying biosensor development concepts to improve preamplification-free CRISPR/Cas12a-Dx." Analyst 145, no. 14 (2020): 4880–88. http://dx.doi.org/10.1039/d0an00664e.

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5

Wang, Wei, and Sameer Sonkusale. "An Approach for a Wide Dynamic Range Low-Noise Current Readout Circuit." Journal of Low Power Electronics and Applications 10, no. 3 (2020): 23. http://dx.doi.org/10.3390/jlpea10030023.

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Designing low-noise current readout circuits at high speed is challenging. There is a need for preamplification stages to amplify weak input currents before being processed by conventional integrator based readout. However, the high current gain preamplification stage usually limits the dynamic range. This article presents a 140 dB input dynamic range low-noise current readout circuit with a noise floor of 10 fArms/sq(Hz). The architecture uses a programmable bidirectional input current gain stage followed by an integrator-based analog-to-pulse conversion stage. The programmable current gains
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6

Andersson, Daniel, Nina Akrap, David Svec, et al. "Properties of targeted preamplification in DNA and cDNA quantification." Expert Review of Molecular Diagnostics 15, no. 8 (2015): 1085–100. http://dx.doi.org/10.1586/14737159.2015.1057124.

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7

Kane, S., J. May, J. Miyamoto, and I. Shipsey. "An aging study of a MICROMEGAS with GEM preamplification." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 515, no. 1-2 (2003): 261–65. http://dx.doi.org/10.1016/j.nima.2003.09.008.

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8

Razavi, M., and J. H. Shapiro. "Wireless optical communications via diversity reception and optical preamplification." IEEE Transactions on Wireless Communications 4, no. 3 (2005): 975–83. http://dx.doi.org/10.1109/twc.2005.847102.

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9

D'Aquila, Richard T., Lawrence J. Bechtel, Joseph A. Videler, Joseph J. Eron, Paul Gorczyca, and Joan C. Kaplan. "Maximizing sensitivity and specificity of PCR by preamplification heating." Nucleic Acids Research 19, no. 13 (1991): 3749. http://dx.doi.org/10.1093/nar/19.13.3749.

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10

Zahn, Hans, Adi Steif, Emma Laks, et al. "Scalable whole-genome single-cell library preparation without preamplification." Nature Methods 14, no. 2 (2017): 167–73. http://dx.doi.org/10.1038/nmeth.4140.

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11

Bencheikh, K., O. Lopez, I. Abram, and J. A. Levenson. "Improvement of photodetection quantum efficiency by noiseless optical preamplification." Applied Physics Letters 66, no. 4 (1995): 399–401. http://dx.doi.org/10.1063/1.114201.

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12

Kärtner, F. X., and H. A. Haus. "QND-measurement of twin beams by phase sensitive preamplification." Applied Physics B: Lasers and Optics 64, no. 2 (1997): 219–23. http://dx.doi.org/10.1007/s003400050167.

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13

Del Gaudio, S., A. Cirillo, G. Di Bernardo, U. Galderisi, and M. Cipollaro. "A preamplification approach to GMO detection in processed foods." Analytical and Bioanalytical Chemistry 396, no. 6 (2009): 2135–42. http://dx.doi.org/10.1007/s00216-009-3199-5.

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14

Del Gaudio, Stefania, Alessandra Cirillo, Giovanni Di Bernardo, et al. "Preamplification Procedure for the Analysis of Ancient DNA Samples." Scientific World Journal 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/734676.

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In ancient DNA studies the low amount of endogenous DNA represents a limiting factor that often hampers the result achievement. In this study we extracted the DNA from nine human skeletal remains of different ages found in the Byzantine cemetery of Abdera Halkidiki and in the medieval cemetery of St. Spiridion in Rhodes (Greece). Real-time quantitative polymerase chain reaction (qPCR) was used to detect in the extracts the presence of PCR inhibitors and to estimate the DNA content. As mitochondrial DNA was detected in all samples, amplification of nuclear targets, as amelogenin and the polymor
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15

Itoh, Y., and R. Kobayashi. "Evaluation of ABO and Lewis genotypes using primer extension preamplification." Forensic Science International 113, no. 1-3 (2000): 139–41. http://dx.doi.org/10.1016/s0379-0738(00)00275-9.

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16

Delbart, A., J. Derré, Y. Giomataris, F. Jeanneau, and I. Papadopoulos. "Performance of MICROMEGAS with preamplification at high intensity hadron beams." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 478, no. 1-2 (2002): 205–9. http://dx.doi.org/10.1016/s0168-9002(01)01758-2.

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17

De Souza, K., and T. P. Newson. "Brillouin-based fiber-optic distributed temperature sensor with optical preamplification." Optics Letters 25, no. 18 (2000): 1331. http://dx.doi.org/10.1364/ol.25.001331.

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18

Lewin, Peter A., Mark E. Schafer, and Robert C. Chivers. "Factors affecting the choice of preamplification for ultrasonic hydrophone probes." Ultrasound in Medicine & Biology 13, no. 3 (1987): 141–48. http://dx.doi.org/10.1016/0301-5629(87)90142-6.

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19

Lunyak, Victoria V., Michael Ezrokhi, Heidi S. Smith, and Susan A. Gerbi. "Developmental Changes in the Sciara II/9A Initiation Zone for DNA Replication." Molecular and Cellular Biology 22, no. 24 (2002): 8426–37. http://dx.doi.org/10.1128/mcb.22.24.8426-8437.2002.

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ABSTRACT Developmentally regulated initiation of DNA synthesis was studied in the fly Sciara at locus II/9A. PCR analysis of nascent strands revealed an initiation zone that spans ∼8 kb in mitotic embryonic cells and endoreplicating salivary glands but contracts to 1.2 to 2.0 kb during DNA amplification of DNA puff II/9A. Thus, the amplification origin occurs within the initiation zone used for normal replication. The initiation zone left-hand border is constant, but the right-hand border changes during development. Also, there is a shift in the preferred site for initiation of DNA synthesis d
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20

Abraham, Neeta A., Anne C. Campbell, Warren D. Hirst, and Catherine L. Nezich. "Optimization of small-scale sample preparation for high-throughput OpenArray analysis." Journal of Biological Methods 8, no. 1 (2021): e143. http://dx.doi.org/10.14440/jbm.2021.339.

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OpenArray is one of the most high-throughput qPCR platforms available but its efficiency can be limited by sample preparation methods that are slow and costly. To optimize the sample workflow for high-throughput qPCR processing by OpenArray, small-scale sample preparation methods were compared for compatibility with this system to build confidence in a method that maintains quality and accuracy while using less starting material and saving time and money. This study is the first to show that the Cells-to-CT kit can be used to prepare samples within the dynamic range of OpenArray directly from
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21

Inoue, K. "Gain-clamped fiber amplifier with a short length of preamplification fiber." IEEE Photonics Technology Letters 11, no. 9 (1999): 1108–10. http://dx.doi.org/10.1109/68.784203.

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22

Bouzid, Belloui, M. K. Abdullah, and M. A. Mahdi. "High gain Er3+-doped fiber amplifier with double-pass preamplification stage." Microwave and Optical Technology Letters 48, no. 5 (2006): 866–68. http://dx.doi.org/10.1002/mop.21501.

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23

Casas, Eduardo, and Brian W. Kirkpatrick. "Evaluation of Different Amplification Protocols for Use in Primer-Extension Preamplification." BioTechniques 20, no. 2 (1996): 219–25. http://dx.doi.org/10.2144/96202st02.

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24

Hillier, David I., David N. Winter, and Nicholas W. Hopps. "Pulse generation and preamplification for long pulse beamlines of Orion laser facility." Applied Optics 49, no. 16 (2010): 3006. http://dx.doi.org/10.1364/ao.49.003006.

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25

Hassun, P. A., M. R. B. Mello, L. P. C. Porto, and J. F. Garcia. "Bovine embryo sexing by primer extension preamplification polymerase chain reaction (PEP - PCR)." Theriogenology 51, no. 1 (1999): 398. http://dx.doi.org/10.1016/s0093-691x(99)91957-x.

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26

Fonte, P., V. Peskov, and B. D. Ramsey. "A study of breakdown limits in microstrip gas counters with preamplification structures." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 416, no. 1 (1998): 23–31. http://dx.doi.org/10.1016/s0168-9002(98)00649-4.

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27

Soto, Marcelo A., Gabriele Bolognini, and Fabrizio Di Pasquale. "Long-range simplex-coded BOTDA sensor over 120km distance employing optical preamplification." Optics Letters 36, no. 2 (2011): 232. http://dx.doi.org/10.1364/ol.36.000232.

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28

Zhou, Huijuan, Chongfeng Sun, Mo Chen, Wei Chen, and Zhou Meng. "Characteristics of a Brillouin-erbium fiber laser based on Brillouin pump preamplification." Applied Optics 51, no. 29 (2012): 7046. http://dx.doi.org/10.1364/ao.51.007046.

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29

Arneson, N., S. Hughes, R. Houlston, and S. Done. "Whole-Genome Amplification by Improved Primer Extension Preamplification PCR (I-PEP-PCR)." Cold Spring Harbor Protocols 2008, no. 2 (2008): pdb.prot4921. http://dx.doi.org/10.1101/pdb.prot4921.

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30

Leonhardt, U., and H. Paul. "High-Accuracy Optical Homodyne Detection with Low-Efficiency Detectors: "Preamplification" from Antisqueezing." Physical Review Letters 72, no. 26 (1994): 4086–89. http://dx.doi.org/10.1103/physrevlett.72.4086.

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31

Schaaff, F., Heike Wedemann та Eberhard Schwinger. "Analysis of sex and ΔF508 in single amniocytes using primer extension preamplification". Human Genetics 98, № 2 (1996): 158–61. http://dx.doi.org/10.1007/s004390050180.

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32

Iwanczyk, Jan S. "Advances in mercuric iodide X-ray detectors and low noise preamplification systems." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 283, no. 2 (1989): 208–14. http://dx.doi.org/10.1016/0168-9002(89)91357-0.

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33

Peleg, Ofer, Gad Baneth, Osnat Eyal, Jacob Inbar, and Shimon Harrus. "Use of Chimeric DNA-RNA Primers in Quantitative PCR for Detection of Ehrlichia canis and Babesia canis." Applied and Environmental Microbiology 75, no. 19 (2009): 6393–98. http://dx.doi.org/10.1128/aem.00720-09.

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ABSTRACT To overcome the problem of nonspecific by-products in quantitative PCR (qPCR) assays, we constructed DNA-RNA chimeric primers and evaluated their use in the detection and quantification of the Ehrlichia canis 16S rRNA, Babesia canis Hsp70, and canine β-actin genes. Several RNA bases were incorporated into specific positions in the DNA primers, while no RNA stretches were allowed. qPCR reactions were carried out without preamplification steps. This resulted in decreased formation of undesirable by-products and a 10-fold increase in assay sensitivity.
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34

Sun, Fengzhu, and Michael S. Waterman. "Whole Genome Amplification and Branching Processes." Advances in Applied Probability 29, no. 03 (1997): 629–68. http://dx.doi.org/10.1017/s0001867800028287.

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Whole genome amplification is important for multipoint mapping by sperm or oocyte typing and genetic disease diagnosis. Polymerase chain reaction is not suitable for amplifying long DNA sequences. This paper studies a new technique, designated PEP-primer-extension-preamplification, for amplifying long DNA sequences using the theory of branching processes. A mathematical model for PEP is constructed and a closed formula for the expected target yield is obtained. A central limit theorem and a strong law of large numbers for the number of kth generation target sequences are proved.
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35

Sun, Fengzhu, and Michael S. Waterman. "Whole Genome Amplification and Branching Processes." Advances in Applied Probability 29, no. 3 (1997): 629–68. http://dx.doi.org/10.2307/1428080.

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Whole genome amplification is important for multipoint mapping by sperm or oocyte typing and genetic disease diagnosis. Polymerase chain reaction is not suitable for amplifying long DNA sequences. This paper studies a new technique, designated PEP-primer-extension-preamplification, for amplifying long DNA sequences using the theory of branching processes. A mathematical model for PEP is constructed and a closed formula for the expected target yield is obtained. A central limit theorem and a strong law of large numbers for the number of kth generation target sequences are proved.
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36

Xie, Xin, Tamara Gjorgjieva, Zaynoun Attieh, et al. "Microfluidic Nano-Scale qPCR Enables Ultra-Sensitive and Quantitative Detection of SARS-CoV-2." Processes 8, no. 11 (2020): 1425. http://dx.doi.org/10.3390/pr8111425.

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A major challenge in controlling the COVID-19 pandemic is the high false-negative rate of the commonly used RT-PCR methods for SARS-CoV-2 detection in clinical samples. Accurate detection is particularly challenging in samples with low viral loads that are below the limit of detection (LoD) of standard one- or two-step RT-PCR methods. In this study, we implemented a three-step approach for SARS-CoV-2 detection and quantification that employs reverse transcription, targeted cDNA preamplification, and nano-scale qPCR based on a commercially available microfluidic chip. Using SARS-CoV-2 synthetic
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37

Thomson, Emma, Camilla L. C. Ip, Anjna Badhan, et al. "Comparison of Next-Generation Sequencing Technologies for Comprehensive Assessment of Full-Length Hepatitis C Viral Genomes." Journal of Clinical Microbiology 54, no. 10 (2016): 2470–84. http://dx.doi.org/10.1128/jcm.00330-16.

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Affordable next-generation sequencing (NGS) technologies for hepatitis C virus (HCV) may potentially identify both viral genotype and resistance genetic motifs in the era of directly acting antiviral (DAA) therapies. This study compared the ability of high-throughput NGS methods to generate full-length, deep, HCV sequence data sets and evaluated their utility for diagnostics and clinical assessment. NGS methods using (i) unselected HCV RNA (metagenomics), (ii) preenrichment of HCV RNA by probe capture, and (iii) HCV preamplification by PCR implemented in four United Kingdom centers were compar
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38

Kays, J. S., Chao Li, and G. D. Nicol. "Expression of sphingosine 1-phosphate receptors in the rat dorsal root ganglia and defined single isolated sensory neurons." Physiological Genomics 44, no. 18 (2012): 889–901. http://dx.doi.org/10.1152/physiolgenomics.00053.2012.

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Previously, we demonstrated that sphingosine 1-phosphate (S1P) increased the excitability of small-diameter sensory neurons, in part, through activation of S1P receptor 1 (S1PR1), suggesting that other S1PRs can modulate neuronal excitability. Therefore, studies were undertaken to establish the expression profiles of S1PRs in the intact dorsal root ganglion (DRG) and in defined single isolated sensory neurons. To determine mRNA expression of S1PRs in the DRG, SYBR green quantitative PCR (qPCR) was used. To determine the expression of S1PR mRNAs in single neurons of defined diameters, a preampl
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39

Baak-Pablo, Renee, Vincent Dezentje, Henk-Jan Guchelaar, and Tahar van der Straaten. "Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification." Journal of Molecular Diagnostics 12, no. 6 (2010): 746–49. http://dx.doi.org/10.2353/jmoldx.2010.100047.

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40

Dark, J., G. Nunn, J. D. Cressler, and D. Davidović. "Increasing the signal-to-noise ratio of magnetic tunnel junctions by cryogenic preamplification." Journal of Applied Physics 125, no. 16 (2019): 163902. http://dx.doi.org/10.1063/1.5059326.

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41

Dimaras, Pantelis, Oskan Tasinov, Desislava Ivanova, Yoana Kiselova-Kaneva, and Diana Ivanova. "Increasing detection sensitivity of low copy number transcripts through preamplification of cDNA molecules." Scripta Scientifica Medica 49, no. 2 (2017): 28. http://dx.doi.org/10.14748/ssm.v49i2.2180.

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42

Al-Mansoori, Mohammed Haydar, and Mohd A. Mahdi. "Tunable range enhancement of Brillouin-erbium fiber laser utilizing Brillouin pump preamplification technique." Optics Express 16, no. 11 (2008): 7649. http://dx.doi.org/10.1364/oe.16.007649.

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43

Sánchez-García, Jorge, Dominique Gallardo, Joaquima Navarro, et al. "A versatile strategy for preimplantation genetic diagnosis of haemophilia A based on F8-gene sequencing." Thrombosis and Haemostasis 96, no. 12 (2006): 839–45. http://dx.doi.org/10.1160/th06-05-069.

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SummaryPreimplantation genetic diagnosis (PGD) of hemophilia A (HA) and other X-linked diseases through sex selection implies that male embryos will be systematically discarded, even though 50% are unaffected. The objective of the present work was to develop a PGD protocol for direct mutation identification that could be applied to first polar bodies (1PBs) in several HA clinical cases. Single buccal cells from controls and patients, and 1PBs were subjected to primer extension preamplification (PEP) PCR followed by amplification of F8 gene coding and intronic flanking regions, and direct seque
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44

Mengual, Lourdes, Moisès Burset, Mercedes Marín-Aguilera, María Ribal, and Antonio Alcaraz. "Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays." BMC Research Notes 1, no. 1 (2008): 21. http://dx.doi.org/10.1186/1756-0500-1-21.

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45

Curry, M. J., T. D. England, N. C. Bishop, et al. "Cryogenic preamplification of a single-electron-transistor using a silicon-germanium heterojunction-bipolar-transistor." Applied Physics Letters 106, no. 20 (2015): 203505. http://dx.doi.org/10.1063/1.4921308.

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46

Maciejewska, Agnieszka, Joanna Jakubowska, and Ryszard Pawłowski. "Different Whole-Genome Amplification Methods as a Preamplification Tool in Y-Chromosome Loci Analysis." American Journal of Forensic Medicine and Pathology 35, no. 2 (2014): 140–44. http://dx.doi.org/10.1097/paf.0000000000000093.

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47

Tobben, H., and M. Karbsek. "Experimental and numerical results of optical preamplification in a laser Doppler anemometer receiving head." IEEE Transactions on Instrumentation and Measurement 49, no. 1 (2000): 10–13. http://dx.doi.org/10.1109/19.836300.

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48

Hemmerling, J., J. Jansen, M. Müller, and D. Haller. "Fetal gut laser microdissection in combination with RNA preamplification enables epithelial-specific transcriptional profiling." Journal of Immunological Methods 416 (January 2015): 189–92. http://dx.doi.org/10.1016/j.jim.2014.11.008.

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49

Pusch, Carsten M., Graeme J. Nicholson, Lutz Bachmann, and Michael Scholz. "Degenerate Oligonucleotide-Primed Preamplification of Ancient DNA Allows the Retrieval of Authentic DNA Sequences." Analytical Biochemistry 279, no. 1 (2000): 118–22. http://dx.doi.org/10.1006/abio.1999.4463.

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50

Kong, H. J., S. S. Lee, H. S. Kim, et al. "High-power Nd3+:glass laser system in KAIST (Sinmyung I)." Laser and Particle Beams 15, no. 2 (1997): 317–24. http://dx.doi.org/10.1017/s0263034600010612.

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A high-power Nd3+:glass laser system has been constructed and tested. This system consists of a master oscillator, a four-pass amplifier for preamplification, and five-stage amplifiers. The system has been demonstrated in excess of 80 J (2 TW) at 40-ps pulse duration. Final laser beam quality was quite good due to the compensation of the polarization distortion in the four-pass preamplifier, the minimization of the diffraction effect by the image relaying, and the elimination of high spatial frequency components by the spatial filtering. This enables us to obtain high laser output power withou
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