Relevant bibliographies by topics / Preparation of inoculum

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Preparation of inoculum'

Author: Grafiati
Published: 4 June 2021
Last updated: 7 February 2022

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Journal articles on the topic "Preparation of inoculum"

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Webb, C., and S. P. Kamat. "Improving fermentation consistency through better inoculum preparation." World Journal of Microbiology & Biotechnology 9, no. 3 (May 1993): 308–12. http://dx.doi.org/10.1007/bf00383069.

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GONZÁLEZ, H. H. L., A. PACIN, G. BOENTE, E. MARTÍNEZ, and S. RESNIK. "Influence of Inoculum Preparation and Volume on Growth of Mycotoxigenic Molds." Journal of Food Protection 58, no. 4 (April 1, 1995): 430–33. http://dx.doi.org/10.4315/0362-028x-58.4.430.

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The influence of the volume and the aw of the inoculum, adjusted or not with glycerol to the aw of the medium, on growth of mycotoxigenic species of fungi was determined for Aspergillus flavus, Aspergillus ochraceus, Penicillium citrinum, Penicillium viridicatum, Fusarium tricinctum and Microdochium nivale. Statistical analysis of the data was based on the application of multivariate techniques. It was seen that at constant volumes (10 μl), P. viridicatum, A. ochraceus and M. nivale show no significant growth differences whether or not the aw of the inoculum is adjusted; but significant differences were observed with A. flavus, P. citrinum and F. tricinctum. Moreover, significant differences in growth between adjusted and unadjusted aw levels are also present when there are different inoculum volumes for A. flavus and P. citrinum. Thus, it is appropriate to adjust the inoculum to the same aw level as that of the medium or the food to be considered. In all cases, where significant differences were present, greater colony diameters were observed when the aw was adjusted. With the same inoculum preparation, different inoculum volumes also affect fungus growth, producing greater colony diameters with increments in the volume.
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REID, ANNE N., COURTNEY CONKLIN, KIMBERLY BEATON, NORA DONAHUE, EMILY JACKSON, BRIANNA LoCASCIO, CRISTINA MARSOCCI, EMILY SZEMREYLO, and KATLIN SZEMREYLO. "Inoculum Preparation Conditions Influence Adherence of Salmonella enterica Serovars to Red Leaf Lettuce (Lactuca sativa)." Journal of Food Protection 84, no. 5 (January 7, 2021): 857–68. http://dx.doi.org/10.4315/jfp-20-301.

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ABSTRACT Salmonella enterica has been increasingly linked to outbreaks involving consumption of fresh produce. Although researchers have identified genes whose products are involved in mediating S. enterica–plant interactions, the use of various experimental approaches, serovars, and plant types has generated variable and conflicting data. The purpose of this study was to determine whether conditions under which inocula are prepared for in vitro plant interaction studies influence the outcome of these studies. Seven S. enterica serovars were grown in media that differed in salinity and physical state with incubation at 25 or 37°C. These cultures were then used to inoculate red leaf lettuce, and adherent microbes were subsequently recovered. Although all Salmonella serovars were influenced by inoculum preparation conditions, the amount of variation differed. Analysis of pooled serovar data revealed that inocula prepared from either agar plates or biphasic cultures had higher levels of interaction with red leaf lettuce than those prepared from broth cultures. Incubation at 37°C enhanced adherence after 30 s or 5 days of contact time, and adherence after 1 h of contact time was increased in low-salt medium. Broth inoculum cultures were highly influenced by medium salinity and incubation temperature, whereas plate and biphasic inoculum cultures were only minimally affected. Therefore, inocula prepared from bacteria grown on plates or in biphasic culture would be most suitable for evaluation of strategies used to interfere with plant-Salmonella interactions. However, pooled data mask serovar-specific responses, and care should be taken when extrapolating these findings to individual serovars. The previous association of a serovar with outbreaks involving leafy greens was not correlated with levels of interaction with red leaf lettuce, suggesting that the occurrence of these serovars in or on these commodities does not reflect their fitness in the plant environment. HIGHLIGHTS
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SIMPSON, CATHERINE A., IFIGENIA GEORNARAS, YOHAN YOON, JOHN A. SCANGA, PATRICIA A. KENDALL, and JOHN N. SOFOS. "Effect of Inoculum Preparation Procedure and Storage Time and Temperature on the Fate of Listeria monocytogenes on Inoculated Salami." Journal of Food Protection 71, no. 3 (March 1, 2008): 494–501. http://dx.doi.org/10.4315/0362-028x-71.3.494.

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Although dry/semidry fermented sausages are characterized as being of low-to-moderate risk for human listeriosis on a per-serving and per-annum basis, data are lacking relative to the fate of postprocessing Listeria monocytogenes contamination during storage of such products. This study evaluated the effect of inoculum preparation and storage conditions on the fate of L. monocytogenes on vacuum-packaged salami. Commercially produced salami was sliced and inoculated (4 ± 1.3 log CFU/cm2) with one of four types of inocula. All inocula consisted of the same 10-strain L. monocytogenes composite, cultivated as individual strains prior to mixing for inoculation. Active cultures of individual strains were prepared (30°C, 24 h) in either tryptic soy broth (containing 0.25% glucose) plus 0.6% yeast extract (TSBYE), tryptic soy broth without glucose plus 0.6% yeast extract (TSBYE–G), TSBYE–G plus 1% glucose (TSBYE+G), or in TSBYE, and then habituated (7°C, 72 h) in sterile salami homogenate (10% [wt/wt] with distilled water). Inoculated salami slices were vacuum packaged, stored at 4, 12, or 25°C, and analyzed (three samples per treatment in each of two replicates) periodically for surviving bacterial counts. In general, pathogen levels decreased during storage and reached levels below the detection limit (−0.4 log CFU/cm2) between 27 and 90 days of storage, depending on temperature of storage and inoculum type. Death rates (log CFU/cm2/day) were found to increase as storage temperature increased, with the exception of the acid-adapted (TSBYE+G) cells, which decreased more rapidly at 4°C than at 12 or 25°C. The habituated inoculum was inactivated at a faster rate than other inocula at 12 and 25°C, but performed similarly to nonadapted (TSBYE–G) and partially acid-adapted (TSBYE) inocula at 4°C. These data may be used to supplement existing information for use in future risk assessments.
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Pfaller, M. A., L. Burmeister, M. S. Bartlett, and M. G. Rinaldi. "Multicenter evaluation of four methods of yeast inoculum preparation." Journal of Clinical Microbiology 26, no. 8 (1988): 1437–41. http://dx.doi.org/10.1128/jcm.26.8.1437-1441.1988.

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Supakod, S., and A. Wongwicharn. "Cheap Media for Inoculum Preparation of Acetic Acid Bacteria." Advanced Materials Research 506 (April 2012): 575–78. http://dx.doi.org/10.4028/www.scientific.net/amr.506.575.

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Acetic acid bacteria are used in the production of many kinds of food product: Acetobacter aceti, a starter culture of vinegar fermentation; Acetobacter xylinum, a starter culture of bacterial cellulose production (nata de coco). The objective of this research was to find cheap media for the preparation of seed culture of these bacteria. Coconut water, banana juice and a mixture of coconut water and banana juice (ratio 1:1) were used as inoculation media and compared with HS and GEY, the commonly used media for acetic acid bacteria. Acetobacter aceti TISTR102, Acetobacter xylinum TISTR975, Acetobacter xylinum AGR60 and the isolated Acetobacter xylinum Coc5 were used as the test strains. The pH and total sugar of all media were adjusted as the control media (HS & GEY) at 5.0 and 2% (w/v), respectively. The results found that all strains grew well in each medium and viable cells achieved the level of at least 106 CFU/ml when cultured for 12 hours at 30°C, 200 rpm. The result shows that constitutes of agricultural product such as coconut water and banana juice can be used as cheap inoculation media for acetic acid bacteria.
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Guarro, J., I. Pujol, C. Aguilar, C. Llop, and J. Fernandez-Ballart. "Inoculum preparation for in-vitro susceptibility testing of filamentous fungi." Journal of Antimicrobial Chemotherapy 42, no. 3 (September 1, 1998): 385–87. http://dx.doi.org/10.1093/jac/42.3.385.

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Sobkowiak, Sylwester, and Jadwiga Śliwka. "Phytophthora Infestans: Isolation of Pure Cultures, Storage and Inoculum Preparation." Plant Breeding and Seed Science 76, no. 1 (December 20, 2017): 9–15. http://dx.doi.org/10.1515/plass-2017-0015.

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Abstract Phytophthora infestans causes potato and tomato late blight, economically the most important disease of these plant species. The Oomycete pathogen is frequently sampled, isolated to pure cultures, stored, and characterized. The knowledge of its diversity, migrations and evolution is essential for breeding resistant plants and for designing appropriate control strategies. The article presents methods for collection, storage and preparation of P. infestans isolates for inoculation of plant tissues, based on the publication by Zarzycka (2001), later updated and modified.
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Sautour, M., P. Dantigny, M. C. Guilhem, and M. Bensoussan. "Influence of inoculum preparation on the growth of Penicillium chrysogenum." Journal of Applied Microbiology 95, no. 5 (November 2003): 1034–38. http://dx.doi.org/10.1046/j.1365-2672.2003.02073.x.

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Wang, Z. G., D. Xiang, X. B. Wang, and C. F. Li. "Preparation of an inoculum ofGluconacetobacter xylinuswithout mutants in shaken culture." Journal of Applied Microbiology 121, no. 3 (July 7, 2016): 713–20. http://dx.doi.org/10.1111/jam.13193.

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Dissertations / Theses on the topic "Preparation of inoculum"

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Oliveira, Cristina Duda de [UNESP]. "Enxertia de plantas de pimentão em Capsicum spp. no manejo de nema tóides de galha." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/105253.

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Made available in DSpace on 2014-06-11T19:33:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-22Bitstream added on 2014-06-13T19:23:54Z : No. of bitstreams: 1 oliveira_cd_dr_jabo_prot.pdf: 6450834 bytes, checksum: 54ef6c225a3ecd6cbc9589a230eb0ac4 (MD5)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O presente trabalho teve por objetivos avaliar: 1 - a utilização de aspersão de água e o repouso das suspensões de ovos e juvenis de Meloídogyne spp. pelos períodos de O; 5; 10; 15; 20; 25 e 30 minutos, na eliminação de espumas retidas sobre estes; 2 - a resistência a Me/oidogyne incogníta de 64 genótipos de pimenta Capsícum chínense; cinco da C. annuum; dois da C. baccatum e um da C. frutescens, e de dez cultivares comerciais de pimentão; 3 - a resistência a M. javaníca e a M. mayaguensis dos genótipos resistentes a M. íncognita; 4 - a compatibilidade da enxertia, dos genótipos resistentes a M. íncogníta, com os híbridos de pimentão Rubia R, Margarita e Maximos, nas fases de muda, de crescimento e na produtiva, e 5 - a manutenção da resistência dos porta-enxertos a M. íncogníta, ao final do ciclo da cultura. Os experimentos de aspersão de água e de repouso das suspensões foram conduzidos no Laboratório de Nematologia da UNESP-FCAV, em Jaboticabal-SP, no ano de 2004. Após a trituração das raízes de Capsicum spp., no experimento de aspersão de água, as suspensões foram vertidas na peneira com orifícios de 0,074 mm (200 mesh) sobre a peneira com orifícios de 0,025 mm (500 mesh) e lavadas, sendo as espumas, retidas sobre os ovos e juvenis na peneira de 500 mesh, aspergidas ou não com 50 mL de água, por 20 segundos. No experimento de repouso, após a trituração, as suspensões ficaram em repouso pelos períodos estabelecidos, sendo, na seqüência, vertidas sobre as peneiras e lavadas. Os experimentos de resistência de Capsicum spp. a Meloidogyne spp., de compatibilidade de enxertia e de manutenção da resistência a nematóides de galha, foram conduzidos em casa de vegetação, em Jaboticabal-SP, entre os anos de 2004 e 2005...
The aim of the present work was to study: 1 - the utilization of water sprinkling and letting suspensions of eggs and juveniles of Meloidogyne spp. stand for periods of O; 5; 10; 15; 20; 25 and 30 min, to eliminate foam remaining on them; 2 resistance to Me/oidogyne incognita in 64 pepper genotypes of the species Capsicum chinense, five of C. Annuum, two of C. baccatum and one of C. frutescens, and of ten commercial pepper cultivars; 3 - resistance to M. javanica and M. mayaguensis in genotypes resistant to M. incognita; 4 - compatibility of grafting of genotypes resistant to M. incognita with the bell pepper hybrids Rubia R, Margarita and Maximos, during seedling, growth and productive phases; and 5 - maintenance of resistance of rootstocks to M. incognita at the end of the cultivation cycle. The experiments studying water sprinkling and letting suspensions stand were conducted in the Nematology Laboratory of UNESP-FCAV, at Jaboticabal-SP, during 2004. In nematode application with water sprinkling, the roots of Capsicum spp. were triturated, and the suspensions were poured over a sieve with a pore size of 0.074 mm (200 mesh) on top of a sieve with a pore size of 0.025 mm (500 mesh) and washed; the foam retained on the eggs and juveniles on the 500-mesh was sprinkled or not using 50 mL of water. In the experiment of letting suspensions stand, after trituration the suspensions were allowed to stand for the periods established, after which they were poured over the sieves and washed. The experiments on the resistance of Capsicum spp. to Meloidogyne spp., the compatibility of grafting and the maintenance of resistance to root-knot nematodes were conducted in a greenhouse, at Jaboticabal-SP, between 2004 and 2005. In experiments on resistance of the genotypes to Meloidogyne spp., ten plants were used per genotype, where each plant was considered a repetition... (Complete abstract click electronic access below)
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Oliveira, Cristina Duda de. "Enxertia de plantas de pimentão em Capsicum spp. no manejo de nema tóides de galha /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/105253.

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Resumo: O presente trabalho teve por objetivos avaliar: 1 - a utilização de aspersão de água e o repouso das suspensões de ovos e juvenis de Meloídogyne spp. pelos períodos de O; 5; 10; 15; 20; 25 e 30 minutos, na eliminação de espumas retidas sobre estes; 2 - a resistência a Me/oidogyne incogníta de 64 genótipos de pimenta Capsícum chínense; cinco da C. annuum; dois da C. baccatum e um da C. frutescens, e de dez cultivares comerciais de pimentão; 3 - a resistência a M. javaníca e a M. mayaguensis dos genótipos resistentes a M. íncognita; 4 - a compatibilidade da enxertia, dos genótipos resistentes a M. íncogníta, com os híbridos de pimentão Rubia R, Margarita e Maximos, nas fases de muda, de crescimento e na produtiva, e 5 - a manutenção da resistência dos porta-enxertos a M. íncogníta, ao final do ciclo da cultura. Os experimentos de aspersão de água e de repouso das suspensões foram conduzidos no Laboratório de Nematologia da UNESP-FCAV, em Jaboticabal-SP, no ano de 2004. Após a trituração das raízes de Capsicum spp., no experimento de aspersão de água, as suspensões foram vertidas na peneira com orifícios de 0,074 mm (200 mesh) sobre a peneira com orifícios de 0,025 mm (500 mesh) e lavadas, sendo as espumas, retidas sobre os ovos e juvenis na peneira de 500 mesh, aspergidas ou não com 50 mL de água, por 20 segundos. No experimento de repouso, após a trituração, as suspensões ficaram em repouso pelos períodos estabelecidos, sendo, na seqüência, vertidas sobre as peneiras e lavadas. Os experimentos de resistência de Capsicum spp. a Meloidogyne spp., de compatibilidade de enxertia e de manutenção da resistência a nematóides de galha, foram conduzidos em casa de vegetação, em Jaboticabal-SP, entre os anos de 2004 e 2005... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of the present work was to study: 1 - the utilization of water sprinkling and letting suspensions of eggs and juveniles of Meloidogyne spp. stand for periods of O; 5; 10; 15; 20; 25 and 30 min, to eliminate foam remaining on them; 2 resistance to Me/oidogyne incognita in 64 pepper genotypes of the species Capsicum chinense, five of C. Annuum, two of C. baccatum and one of C. frutescens, and of ten commercial pepper cultivars; 3 - resistance to M. javanica and M. mayaguensis in genotypes resistant to M. incognita; 4 - compatibility of grafting of genotypes resistant to M. incognita with the bell pepper hybrids Rubia R, Margarita and Maximos, during seedling, growth and productive phases; and 5 - maintenance of resistance of rootstocks to M. incognita at the end of the cultivation cycle. The experiments studying water sprinkling and letting suspensions stand were conducted in the Nematology Laboratory of UNESP-FCAV, at Jaboticabal-SP, during 2004. In nematode application with water sprinkling, the roots of Capsicum spp. were triturated, and the suspensions were poured over a sieve with a pore size of 0.074 mm (200 mesh) on top of a sieve with a pore size of 0.025 mm (500 mesh) and washed; the foam retained on the eggs and juveniles on the 500-mesh was sprinkled or not using 50 mL of water. In the experiment of letting suspensions stand, after trituration the suspensions were allowed to stand for the periods established, after which they were poured over the sieves and washed. The experiments on the resistance of Capsicum spp. to Meloidogyne spp., the compatibility of grafting and the maintenance of resistance to root-knot nematodes were conducted in a greenhouse, at Jaboticabal-SP, between 2004 and 2005. In experiments on resistance of the genotypes to Meloidogyne spp., ten plants were used per genotype, where each plant was considered a repetition... (Complete abstract click electronic access below)
Orientadora: Leila Trevizan Braz
Coorientador: David Ariovaldo Benzatto
Coorientador: Jaime Maia dos Santos
Banca: Arthur Bernardes Cecílio Filho
Banca: Clélia Aparecida Iunes Lapera
Banca: Rumy Goto
Banca: Izabel Cristina Leite
Doutor
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Murray, Dillon SD. "Influence of Inoculum Preparation upon Sensitivity of Common Food Borne Pathogens to Emulsion Based Antimicrobials." 2016. https://scholarworks.umass.edu/masters_theses_2/330.

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Antimicrobial optimization procedures use the most resistant bacterial culture that could be present in the food to determine the levels of treatment needed to ensure safety. These procedures usually only focus on one method of preparing these inoculums for testing despite prior research showing that the preparation of the culture can influence how the culture reacts to a treatment. In this work, planktonic cells grown in a liquid media and sessile cells grown on a similar solid media were subjected to identical emulsion based antimicrobial systems. The cultures were monitored over time and their numbers periodically enumerated. Weibullian destruction models were used to characterize bacterial death and the different inoculum preparations were separated using ANOVA statistical tests. Using these models highly significant differences between the different sessile and planktonic methods of growth were found. This difference was also found to not be related to the production of curli used in biofilm formation. These results suggest that the methods of inoculum preparation can be a significant factor in bacterial survival, a factor that should be included in food antimicrobial optimization procedures.
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LIN, YU-TING, and 林煜庭. "Inoculum Characteristics of Enzyme Profile Using API-ZYM Analysis Kit as well as Preparation and Analysis of Polysaccharides from Commercial Products by Trametes versicolor." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/f86z8s.

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碩士
大葉大學
生物產業科技學系
104
Trametes versicolor (Coriolus versicolor) is a small polypore fungus that is an important part of the forest ecology as a recycler of dead and dying trees in forests throughout the world. Trametes versicolor is not only used as traditional Chinese medicine, the mycelium of Trametes versicolor produced polysaccharide complexes-glycopeptides have also been developed as "pharmaceutical". The main purpose of this study is as inoculum characteristics of enzyme profile using API-ZYM analysis kit by Trametes versicolor, investigating supernatant of submerged culture, comparing colonies cultured in plate with mycelia in enzyme profile between different generations, finger print, as well as preparation (dialysis, enzyme treatment time and thawing factor) and analysis of polysaccharides from commercial products by Trametes versicolor. Furthermore, analysis polysaccharides of molecular weight and the differences in monosaccharide composition. Results of show the different Trametes versicolor generations inoculum in submerged culture of supernatant and mycelium colonies, API-ZYM kit multi-enzyme analysis of enzyme profile of activity and the strength of the performance index. Enzyme profile of colonies shows better then submerged culture of supernatant and mycelium, and activity index is also strong, adapted to judge the algebraic difference in culturing fungi strains and quality control of various fungi basis. The supernatant and the mycelium extract with submerged culture produce different algebraic fingerprint, there is no significant difference between them, profile are presented five major peaks. Determining on profiling, compare mycelium extract and submerged culture of supernatant by Trametes versicolor in the dispersion, the main concentrated of the peak is simply judge the stability of species inoculum of quality. Using API-ZYM enzyme profile can be identified as existence or absence of wild collection among Trametes versicolor strains.The three methods to extract respectively (1) dialysis (2) non-alcoholic precipitation (3) alcohol precipitates analytical sample method, it shows three methods to " non-alcoholic precipitation " of polysaccharide content have the better resulte, and with "dialysis" and " alcohol precipitates" analysis of different product samples, the resulting data have high and low, polysaccharide content in "dialysis" Analysis of comparing "alcohol precipitates Act" is low. Dialysis samples, the time and thawing conditions enzyme treatment may affect the results of analysis of the polysaccharide from Trametes versicolor. The same source material, but different separation and purification will affect the proportion of fruiting bodies and mycelia from Trametes versicolor with monosaccharide content of the fermentation broth and the molecular weight range will be different. Based on the results, API-ZYM kit can be used to analyze Trametes versicolor LH-1 strain characteristics of different generations and as the basis for quality control of the inoculum, PSK related products commercially available phenol-sulfuric acid method when samples were analyzed for polysaccharide content of the sample pretreatment significantly affect the results of its analysis.
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Book chapters on the topic "Preparation of inoculum"

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Mark, G. L., J. Murphy, and Alan C. Cassells. "Microbial Characterisation and Preparation of Inoculum for in vitro Mycorrhization of Strawberry in Autotrophic Culture." In Pathogen and Microbial Contamination Management in Micropropagation, 345–50. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-015-8951-2_42.

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Sood, S., R. Singhal, S. Bhat, and A. Kumar. "Inoculum Preparation." In Comprehensive Biotechnology, 151–64. Elsevier, 2011. http://dx.doi.org/10.1016/b978-0-08-088504-9.00090-8.

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Sood, S., R. Singhal, S. Bhat, and A. Kumar. "Inoculum Preparation." In Comprehensive Biotechnology, 230–43. Elsevier, 2011. http://dx.doi.org/10.1016/b978-0-444-64046-8.00076-8.

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Kropáček, K., and P. Cudlín. "Preparation of Granulated Mycorrhizal Inoculum and Its Use in Forest Nurseries." In Interrelationships between Microorganisms and Plants in Soil, Proceedings of an International Symposium Liblice, 177–82. Elsevier, 1989. http://dx.doi.org/10.1016/s0166-2481(08)70212-5.

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Conference papers on the topic "Preparation of inoculum"

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Savoskina, O. A., S. I. Chebanenko, and Z. K. Kurbanova. "Micoherbicides and growth regulators in the cultivation of flax." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-149.

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Develop a biological preparation (a mixture of inoculum Urocystis agropyri /guack Agropyrum repens/ and Puccinia suaveollens /fragrant rust Cirsium setosum/) reduces the contamination of crops named weeds/. Growth regulators - Artafit and Lostor - effective against bacterial blight, Colletotrichum lini, Ozonium vinogradovi. It was shown that the and boost yields of flax.
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