Academic literature on the topic 'Preserved gene sequences'

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Journal articles on the topic "Preserved gene sequences"

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Corinaldesi, Cinzia, Marco Barucca, Gian Marco Luna, and Antonio DellAnno. "Preservation, origin and genetic imprint of extracellular DNA in permanently anoxic deep-sea sediments." Molecular ecology 20, no. 462 (2010): 454. https://doi.org/10.1111/j.1365-294X.2010.04958.x.

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Molecular approaches that target the total DNA pool recovered from permanently anoxic marine ecosystems have revealed an extraordinary diversity of prokaryotes and unicellular eukaryotes. However, the presence of gene sequences contained within the extracellular DNA pool is still largely neglected. We have investigated the preservation, origin and genetic imprint of extracellular DNA recovered from permanently anoxic deep-sea sediments of the Black Sea. Despite high DNase activities, huge amounts of total extracellular DNA were found in both the surface and sub-surface sediment layers, suggest
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Meek, K., T. Eversole, and J. D. Capra. "Conservation of the most JH proximal Ig VH gene segment (VHVI) throughout primate evolution." Journal of Immunology 146, no. 7 (1991): 2434–38. http://dx.doi.org/10.4049/jimmunol.146.7.2434.

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Abstract The human VHVI gene segment, the sole member of the VHVI gene family, is remarkable in that it is the most D-proximal VH gene segment and is apparently nonpolymorphic. Here we report that the VHVI gene segment has been remarkably preserved in primate evolution. We were unable to detect RFLP among several primates, and nucleotide sequences of several VHVI gene segments showed remarkable conservation. No differences were detected in the nucleotide sequences of the VHVI gene segment from three unrelated chimpanzees. These findings suggest that the VHVI gene segment has been strongly sele
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Sekizaki, Tsutomu, Hiroomi Akashi, and Nobuyuki Terakado. "Nucleotide sequences of the genes for Escherichia coli heat-stable enterotoxin I of bovine, avian, and porcine origins." American Journal of Veterinary Research 46, no. 4 (1985): 909–12. https://doi.org/10.2460/ajvr.1985.46.04.909.

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SUMMARY The nucleotide sequences of cloned Escherichia coli heat-stable toxin 1 (STI) genes isolated from bovine, avian, and porcine origins were determined. They were found to be almost identical to that of Tn1681. The nucleotide sequences were completely preserved in bovine and avian genes, whereas the porcine gene had different sequences at 3 positions in the external region of STI structural genes, as compared with Tn1681. The amino acid sequences of the STI genes of the 3 animal origins corresponded to STIa, which had initially been found in a bovine strain.
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Medeiros, Camila de, Gilberto Aguiar Pereira, Janyeli Dorini Silva de Freitas, et al. "Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean." Semina: Ciências Agrárias 41, no. 6supl2 (2020): 3067–80. http://dx.doi.org/10.5433/1679-0359.2020v41n6supl2p3067.

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Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products we
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Onele, A. O., A. V. Chasov, T. V. Trifonova, and F. V. Minibayeva. "Ascorbate peroxidase of moss Dicranum scoparium: gene identification, enzyme activity." Доклады Академии наук 489, no. 4 (2019): 424–28. http://dx.doi.org/10.31857/s0869-56524894424-428.

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In present work, the APX gene encoding ascorbate peroxidase in the moss Dicranum scoparium was for the first time cloned and sequenced, high homology of APX with ascorbate peroxidase genes of the mosses Grimmia pilifera and Physcomitrella patens was shown. The structure of the protein was characterized using bioinfomatics approach and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of D. scoparium shoots. When exposed to heat shock, a decrease in the acti-vity of ascorbate peroxidase correlated w
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Amatya, Bhaba. "DNA Barcoding of Cyprinid Fish Chagunius chagunio Hamilton,1822 from Phewa Lake, Nepal." International Journal of Biology 11, no. 4 (2019): 88. http://dx.doi.org/10.5539/ijb.v11n4p88.

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The present study is the first of its type that uses a technique of DNA barcoding to determine identification and relationship of a species of fish from Phewa lake, Nepal. The mitochondrial DNA from two ethanol-preserved samples of fish, randomly collected from Phewa lake, was extracted using Gene AllExgene TMtissue extraction kit. 650 base pair of mitochondrial cytochrome c oxidase subunit 1 (CO1) was amplified using a cocktail of four primers and was sequenced bidirectionaly using Sanger sequence method. The DNA sequences were edited using AliView software. The sequences confirmed Chagunius
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Li, Aihong, Jianbiao Zhou, David Zuckerman, et al. "Sequence analysis of clonal immunoglobulin and T-cell receptor gene rearrangements in children with acute lymphoblastic leukemia at diagnosis and at relapse: implications for pathogenesis and for the clinical utility of PCR-based methods of minimal residual disease detection." Blood 102, no. 13 (2003): 4520–26. http://dx.doi.org/10.1182/blood-2003-05-1455.

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AbstractImmunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements provide clonal markers useful for diagnosis and measurement of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL). We analyzed the sequences of Ig and TCR gene rearrangements obtained at presentation and relapse in 41 children with ALL to study clonal stability, which has important implications for monitoring MRD, during the course of the disease. In 42%, all original Ig and/or TCR sequences were conserved. In 24%, one original sequence was preserved but the other lost, and in 14% the original sequences
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HIROSE, MAMIKO, MASAYUKI OSAWA, and EUICHI HIROSE. "DNA barcoding of hermit crabs of genus Clibanarius Dana, 1852 (Anomura: Diogenidae) in the Ryukyu Islands, southwestern Japan." Zootaxa 2414, no. 1 (2010): 59. http://dx.doi.org/10.11646/zootaxa.2414.1.5.

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The diogenid hermit crab genus Clibanarius Dana, 1852 is a group of decapod crustaceans commonly found in tropical or subtropical shallow waters. The species of the genus are usually characterized by distinct color patterns in life, but the identification of colorless specimens preserved in ethanol is sometimes very difficult. DNA barcoding can facilitate species identification and recognition. In this study, we determined partial sequences of the cytochrome oxidase I (COI) gene of 11 Clibanarius species commonly found in the intertidal zones of the Ryukyu Archipelago, southwestern Japan. The
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Wagner, M., and R. P. Perry. "Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA." Molecular and Cellular Biology 5, no. 12 (1985): 3560–76. http://dx.doi.org/10.1128/mcb.5.12.3560-3576.1985.

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Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant of the TACTAAC motif, which is ubiquitous in yeast introns and known to play a criti
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Wagner, M., and R. P. Perry. "Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA." Molecular and Cellular Biology 5, no. 12 (1985): 3560–76. http://dx.doi.org/10.1128/mcb.5.12.3560.

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Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant of the TACTAAC motif, which is ubiquitous in yeast introns and known to play a criti
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Book chapters on the topic "Preserved gene sequences"

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Myrick, Dorkina. "Perspective Chapter: Ethical Implications and International Human Rights Protections of Gene Editing of DNA Sequences." In Genome Editing - Recent Advances and Applications [Working Title]. IntechOpen, 2025. https://doi.org/10.5772/intechopen.1009025.

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CRISPR-Cas9 studies have implicated gene editing of DNA sequences for medical treatment of diseases such as sickle cell anemia, thalassemia, AIDS, blindness, muscular dystrophy, Huntington’s disease, and cystic fibrosis. Moreover, gene editing technology has contributed to the detection of viruses responsible for the dissemination of communicable diseases such as COVID-19. Despite current scientific progress, many uses of gene editing of DNA sequences are ethically questionable, bordering upon antithetical to principles of human rights protections. The history of genetics, genetic modification
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Srivastava, Yashdeep, Keshawanand Tripathi, and Narendra Kumar. "Synthesis of complementary DNA (C-DNA): Principles, methodology, and applications in gene expression studies." In Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis. Deep Science Publishing, 2025. https://doi.org/10.70593/978-93-49307-52-0_26.

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Complementary DNA (cDNA) synthesis from RNA is a pivotal technique in molecular biology that enables the conversion of RNA molecules into DNA copies (Gubler, and Hoffman, 1983). This process holds immense significance in various research fields, including gene expression analysis, functional genomics, and molecular cloning. By synthesizing cDNA from RNA templates, researchers can access a stable DNA form of RNA sequences, allowing for easier manipulation, amplification, and analysis (Green, and Sambrook, 2012). The synthesis of cDNA from RNA involves the reverse transcription (RT) of RNA molec
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Giannasi, David E. "FEASIBILITY OF OBTAINING COMPARATIVE GENE SEQUENCE DATA FROM PRESERVED AND FOSSIL MATERIALS." In Conservation of Plant Genes. Elsevier, 1992. http://dx.doi.org/10.1016/b978-0-12-044140-2.50010-7.

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Paterson, Andrew. "Application of the baculoviral expression system to signal transduction." In Signal Transduction. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199637218.003.0007.

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Abstract The Baculovirus Expression Vector System (BEYS) is an extremely versatile and powerful laboratory tool (1). In simplest terms, the BEYS allows the forced heterologous expression of recombinant protein in susceptible host strains of insect cells. Gene products from the simplest prokaryotes through to the higher eukaryotes have been expressed successfully in this system. Recombinant proteins are recovered in a folded and active form, and posttranslation modifications, such as phosphorylation, lipid acylation, and simple glycosylation are often preserved. Oligomerization is preserved als
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Bodner, Keith. "Israel’s Story in the Psalms." In The Psalms. Oxford University PressNew York, 2024. http://dx.doi.org/10.1093/oso/9780190916862.003.0004.

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Abstract This chapter probes the ways the Israelite songwriters produced various compositions that invite the community to reimagine important events and to highlight the experience of a particular occasion or perhaps to counter a prevailing ideology or belief system. An important genre is the historical psalms, where a sequence of episodes in Israel’s national history are poetically represented. Nearly a dozen psalms might be classified as historical (or at least contain longer historical reflections or have the characteristics of the genre), and three examples are discussed in this chapter:
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Diamantstein, T. "Interleukin-2 and the interleukin-2 receptor complex." In The Biology and Clinical Applications of Interleukin-2. Oxford University PressOxford, 1990. http://dx.doi.org/10.1093/oso/9780199631377.003.0003.

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Abstract Interleukin 2 (IL-2), originally designated as a T cell growth factor, is a glycoprotein with a molecular weight of 15.4 kD. The genes encoding the human, murine, and bovine IL-2 were cloned, their nucleotide sequences determined, and corresponding amino acid sequences deduced.-3 Human IL-2 consists of 133 amino acids and possesses an intramolecular disulfide bridge (between position 58 and 105) essential for the biological activity of the molecule. The position of the cysteine residues, 58 and 105, are also preserved in other species. These results demonstrate the importance of the f
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Conference papers on the topic "Preserved gene sequences"

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Ren, Z. F. "Nano Materials and Physics." In ASME 4th Integrated Nanosystems Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/nano2005-87045.

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Aligning carbon nanotubes in any way desired is very important for many fundamental and applied research projects. In this talk, I will first discuss how to grow them with controlled diameter, length, spacing, and periodicity using catalyst prepared by magnetron sputtering, electron beam (e-beam) lithography, electrochemical deposition, and nanosphere self-assembly. Then I will present our results of field emission property of both the aligned carbon nanotubes grown on flat substrates and random carbon nanotubes grown on carbon cloth. For the aligned carbon nanotubes arrays, I will present the
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