Log in

Relevant bibliographies by topics / Preserved gene sequences / Journal articles

To see the other types of publications on this topic, follow the link: Preserved gene sequences.

Journal articles on the topic 'Preserved gene sequences'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Preserved gene sequences.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Corinaldesi, Cinzia, Marco Barucca, Gian Marco Luna, and Antonio DellAnno. "Preservation, origin and genetic imprint of extracellular DNA in permanently anoxic deep-sea sediments." Molecular ecology 20, no. 462 (2010): 454. https://doi.org/10.1111/j.1365-294X.2010.04958.x.

Full text

Abstract:

Molecular approaches that target the total DNA pool recovered from permanently anoxic marine ecosystems have revealed an extraordinary diversity of prokaryotes and unicellular eukaryotes. However, the presence of gene sequences contained within the extracellular DNA pool is still largely neglected. We have investigated the preservation, origin and genetic imprint of extracellular DNA recovered from permanently anoxic deep-sea sediments of the Black Sea. Despite high DNase activities, huge amounts of total extracellular DNA were found in both the surface and sub-surface sediment layers, suggest

APA, Harvard, Vancouver, ISO, and other styles

2

Meek, K., T. Eversole, and J. D. Capra. "Conservation of the most JH proximal Ig VH gene segment (VHVI) throughout primate evolution." Journal of Immunology 146, no. 7 (1991): 2434–38. http://dx.doi.org/10.4049/jimmunol.146.7.2434.

Full text

Abstract:

Abstract The human VHVI gene segment, the sole member of the VHVI gene family, is remarkable in that it is the most D-proximal VH gene segment and is apparently nonpolymorphic. Here we report that the VHVI gene segment has been remarkably preserved in primate evolution. We were unable to detect RFLP among several primates, and nucleotide sequences of several VHVI gene segments showed remarkable conservation. No differences were detected in the nucleotide sequences of the VHVI gene segment from three unrelated chimpanzees. These findings suggest that the VHVI gene segment has been strongly sele

APA, Harvard, Vancouver, ISO, and other styles

3

Sekizaki, Tsutomu, Hiroomi Akashi, and Nobuyuki Terakado. "Nucleotide sequences of the genes for Escherichia coli heat-stable enterotoxin I of bovine, avian, and porcine origins." American Journal of Veterinary Research 46, no. 4 (1985): 909–12. https://doi.org/10.2460/ajvr.1985.46.04.909.

Full text

Abstract:

SUMMARY The nucleotide sequences of cloned Escherichia coli heat-stable toxin 1 (STI) genes isolated from bovine, avian, and porcine origins were determined. They were found to be almost identical to that of Tn1681. The nucleotide sequences were completely preserved in bovine and avian genes, whereas the porcine gene had different sequences at 3 positions in the external region of STI structural genes, as compared with Tn1681. The amino acid sequences of the STI genes of the 3 animal origins corresponded to STIa, which had initially been found in a bovine strain.

APA, Harvard, Vancouver, ISO, and other styles

4

Medeiros, Camila de, Gilberto Aguiar Pereira, Janyeli Dorini Silva de Freitas, et al. "Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean." Semina: Ciências Agrárias 41, no. 6supl2 (2020): 3067–80. http://dx.doi.org/10.5433/1679-0359.2020v41n6supl2p3067.

Full text

Abstract:

Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products we

APA, Harvard, Vancouver, ISO, and other styles

5

Onele, A. O., A. V. Chasov, T. V. Trifonova, and F. V. Minibayeva. "Ascorbate peroxidase of moss Dicranum scoparium: gene identification, enzyme activity." Доклады Академии наук 489, no. 4 (2019): 424–28. http://dx.doi.org/10.31857/s0869-56524894424-428.

Full text

Abstract:

In present work, the APX gene encoding ascorbate peroxidase in the moss Dicranum scoparium was for the first time cloned and sequenced, high homology of APX with ascorbate peroxidase genes of the mosses Grimmia pilifera and Physcomitrella patens was shown. The structure of the protein was characterized using bioinfomatics approach and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of D. scoparium shoots. When exposed to heat shock, a decrease in the acti-vity of ascorbate peroxidase correlated w

APA, Harvard, Vancouver, ISO, and other styles

6

Amatya, Bhaba. "DNA Barcoding of Cyprinid Fish Chagunius chagunio Hamilton,1822 from Phewa Lake, Nepal." International Journal of Biology 11, no. 4 (2019): 88. http://dx.doi.org/10.5539/ijb.v11n4p88.

Full text

Abstract:

The present study is the first of its type that uses a technique of DNA barcoding to determine identification and relationship of a species of fish from Phewa lake, Nepal. The mitochondrial DNA from two ethanol-preserved samples of fish, randomly collected from Phewa lake, was extracted using Gene AllExgene TMtissue extraction kit. 650 base pair of mitochondrial cytochrome c oxidase subunit 1 (CO1) was amplified using a cocktail of four primers and was sequenced bidirectionaly using Sanger sequence method. The DNA sequences were edited using AliView software. The sequences confirmed Chagunius

APA, Harvard, Vancouver, ISO, and other styles

7

Li, Aihong, Jianbiao Zhou, David Zuckerman, et al. "Sequence analysis of clonal immunoglobulin and T-cell receptor gene rearrangements in children with acute lymphoblastic leukemia at diagnosis and at relapse: implications for pathogenesis and for the clinical utility of PCR-based methods of minimal residual disease detection." Blood 102, no. 13 (2003): 4520–26. http://dx.doi.org/10.1182/blood-2003-05-1455.

Full text

Abstract:

AbstractImmunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements provide clonal markers useful for diagnosis and measurement of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL). We analyzed the sequences of Ig and TCR gene rearrangements obtained at presentation and relapse in 41 children with ALL to study clonal stability, which has important implications for monitoring MRD, during the course of the disease. In 42%, all original Ig and/or TCR sequences were conserved. In 24%, one original sequence was preserved but the other lost, and in 14% the original sequences

APA, Harvard, Vancouver, ISO, and other styles

8

HIROSE, MAMIKO, MASAYUKI OSAWA, and EUICHI HIROSE. "DNA barcoding of hermit crabs of genus Clibanarius Dana, 1852 (Anomura: Diogenidae) in the Ryukyu Islands, southwestern Japan." Zootaxa 2414, no. 1 (2010): 59. http://dx.doi.org/10.11646/zootaxa.2414.1.5.

Full text

Abstract:

The diogenid hermit crab genus Clibanarius Dana, 1852 is a group of decapod crustaceans commonly found in tropical or subtropical shallow waters. The species of the genus are usually characterized by distinct color patterns in life, but the identification of colorless specimens preserved in ethanol is sometimes very difficult. DNA barcoding can facilitate species identification and recognition. In this study, we determined partial sequences of the cytochrome oxidase I (COI) gene of 11 Clibanarius species commonly found in the intertidal zones of the Ryukyu Archipelago, southwestern Japan. The

APA, Harvard, Vancouver, ISO, and other styles

9

Wagner, M., and R. P. Perry. "Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA." Molecular and Cellular Biology 5, no. 12 (1985): 3560–76. http://dx.doi.org/10.1128/mcb.5.12.3560-3576.1985.

Full text

Abstract:

Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant of the TACTAAC motif, which is ubiquitous in yeast introns and known to play a criti

APA, Harvard, Vancouver, ISO, and other styles

10

Wagner, M., and R. P. Perry. "Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA." Molecular and Cellular Biology 5, no. 12 (1985): 3560–76. http://dx.doi.org/10.1128/mcb.5.12.3560.

Full text

Abstract:

Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant of the TACTAAC motif, which is ubiquitous in yeast introns and known to play a criti

APA, Harvard, Vancouver, ISO, and other styles

11

Spielmann, Nadine, Diane Ilsley, Jian Gu, et al. "The Human Salivary RNA Transcriptome Revealed by Massively Parallel Sequencing." Clinical Chemistry 58, no. 9 (2012): 1314–21. http://dx.doi.org/10.1373/clinchem.2011.176941.

Full text

Abstract:

Abstract BACKGROUND Evaluation of the salivary transcriptome is an emerging diagnostic technology with discriminatory power for disease detection. This study explored massively parallel sequencing for providing nucleotide-level sequence information for each RNA in saliva. METHODS Transcriptome profiling with the SOLiD™ system was applied to RNA isolated from unstimulated cell-free saliva (CFS) and whole saliva (WS) from healthy human volunteers. Sequenced reads were aligned to human genome build 18 and the Human Oral Microbiome Database (HOMD). RESULTS Massively parallel sequencing enabled the

APA, Harvard, Vancouver, ISO, and other styles

12

Ivorová, Silvia, Anna Kopčaková, Peter Pristaš, and Svetlana Kišidayová. "Morphometric and Molecular Analysis of Five-Spine Epidinium Morphotypes Taken from the Rumen of European Bison, Bison bonasus." Life 13, no. 12 (2023): 2350. http://dx.doi.org/10.3390/life13122350.

Full text

Abstract:

An important feature of ruminal ciliates is their phenotypic plasticity, which makes their identification difficult. The common manifestation of the phenotypic plasticity in rumen ciliates is a change in their cell size and caudal spination. We analyzed various morphotypes of Epidinium with five caudal processes (spines) taken from the rumen of European bison (Bison bonasus). In the study, the cluster analysis and K-means analysis of morphometric data could not distinguish very similar morphotypes of Epidinium with five caudal processes. However, the morphotype of E. parvicaudatum prevailed (7

APA, Harvard, Vancouver, ISO, and other styles

13

Widłak, Wiesława, Natalia Vydra, Volha Dudaladava, Dorota Scieglińska, Bolesław Winiarski, and Zdzisław Krawczyk. "The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene." Acta Biochimica Polonica 54, no. 1 (2007): 107–12. http://dx.doi.org/10.18388/abp.2007_3275.

Full text

Abstract:

The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expr

APA, Harvard, Vancouver, ISO, and other styles

14

Malkani, Naila, Saqiba Khalid, Sania Ashraf, et al. "Molecular identification of museum preserved type specimens of fish species using DNA barcoding." Lahore Garrison University Journal of Life Sciences 6, no. 03 (2022): 200–215. http://dx.doi.org/10.54692/lgujls.2022.0603223.

Full text

Abstract:

Type specimens of organisms provide the basis for the identification of species. Proper phenotypic data supported by genetic evidence is crucial for every type of specimen keeping in view its significance. Several methods of species identification both morphological and genetic are being used. DNA barcoding using a fragment of cytochrome c oxidase subunit I (COI) mitochondrial gene is gaining popularity because of its accuracy and efficiency. In this study, six type specimens of endemic fishes from Pakistan preserved at Stephenson Natural History Museum, GC University, Lahore, Pakistan were an

APA, Harvard, Vancouver, ISO, and other styles

15

Richardson, Christine, and Maria Jasin. "Coupled Homologous and Nonhomologous Repair of a Double-Strand Break Preserves Genomic Integrity in Mammalian Cells." Molecular and Cellular Biology 20, no. 23 (2000): 9068–75. http://dx.doi.org/10.1128/mcb.20.23.9068-9075.2000.

Full text

Abstract:

ABSTRACT DNA double-strand breaks (DSBs) may be caused by normal metabolic processes or exogenous DNA damaging agents and can promote chromosomal rearrangements, including translocations, deletions, or chromosome loss. In mammalian cells, both homologous recombination and nonhomologous end joining (NHEJ) are important DSB repair pathways for the maintenance of genomic stability. Using a mouse embryonic stem cell system, we previously demonstrated that a DSB in one chromosome can be repaired by recombination with a homologous sequence on a heterologous chromosome, without any evidence of genome

APA, Harvard, Vancouver, ISO, and other styles

16

Roth, Andreas, Marga Fischer, Mohamed E. Hamid, Sabine Michalke, Wolfgang Ludwig, and Harald Mauch. "Differentiation of Phylogenetically Related Slowly Growing Mycobacteria Based on 16S-23S rRNA Gene Internal Transcribed Spacer Sequences." Journal of Clinical Microbiology 36, no. 1 (1998): 139–47. http://dx.doi.org/10.1128/jcm.36.1.139-147.1998.

Full text

Abstract:

Interspecific polymorphisms of the 16S rRNA gene (rDNA) are widely used for species identification of mycobacteria. 16S rDNA sequences, however, do not vary greatly within a species, and they are either indistinguishable in some species, for example, in Mycobacterium kansasii and M. gastri, or highly similar, for example, in M. malmoense and M. szulgai. We determined 16S-23S rDNA internal transcribed spacer (ITS) sequences of 60 strains in the genus Mycobacterium representing 13 species (M. avium, M. conspicuum, M. gastri, M. genavense, M. kansasii,M. malmoense, M. marinum, M. shimoidei, M. si

APA, Harvard, Vancouver, ISO, and other styles

17

Corinaldesi, C., M. Tangherlini, G. M. Luna, and A. Dell'Anno. "Extracellular DNA can preserve the genetic signatures of present and past viral infection events in deep hypersaline anoxic basins." Proceedings of the Royal Society B: Biological Sciences 281, no. 1780 (2014): 20133299. http://dx.doi.org/10.1098/rspb.2013.3299.

Full text

Abstract:

Deep hypersaline anoxic basins (DHABs) of the Mediterranean Sea are among the most extreme ecosystems on Earth and host abundant, active and diversified prokaryotic assemblages. However, factors influencing biodiversity and ecosystem functioning are still largely unknown. We investigated, for the first time, the impact of viruses on the prokaryotic assemblages and dynamics of extracellular DNA pool in the sediments of La Medee, the largest DHAB found on Earth. We also compared, in La Medee and L'Atalante sediments, the diversity of prokaryotic 16S rDNA sequences contained in the extracellular

APA, Harvard, Vancouver, ISO, and other styles

18

Ge, Jiayun, Yannan Bai, Bo Tang, Dong Wei, and Maolin Yan. "The Gene Signature Associated with Hepatocellular Carcinoma in Patients with Nonalcoholic Fatty Liver Disease." Journal of Oncology 2021 (April 2, 2021): 1–9. http://dx.doi.org/10.1155/2021/6630535.

Full text

Abstract:

Background. Nonalcoholic fatty liver disease (NAFLD) is becoming a critical risk of hepatocellular carcinoma (HCC). As both NAFLD and HCC are heterogeneous diseases, this study aims to identify the similarity between the subtypes of NAFLD and HCC based on gene modules. Methods. Coexpressed gene modules were extracted for both NAFLD and HCC. The association between the coexpressed gene modules of NAFLD and HCC was evaluated by Fisher’s exact test. The overlapping coexpressed gene module was validated in three independent human NAFLD datasets. Furthermore, the preserved gene module was assessed

APA, Harvard, Vancouver, ISO, and other styles

19

GIBSON, W., J. G. PILKINGTON, and J. M. PEMBERTON. "Trypanosoma melophagium from the sheep ked Melophagus ovinus on the island of St Kilda." Parasitology 137, no. 12 (2010): 1799–804. http://dx.doi.org/10.1017/s0031182010000752.

Full text

Abstract:

SUMMARYThe sheep ked has been largely eradicated in the UK but persists in the feral Soay sheep of St Kilda in the Outer Hebrides. Sheep keds transmit Trypanosoma melophagium, but parasitaemias are typically cryptic and this trypanosome has not been recorded in the St Kilda sheep. Trypanosomes were detected by PCR in preserved keds and were also found in gut smears from live keds; one infected gut was used to establish the trypanosome in vitro. Examination of the morphology of bloodstream forms from culture confirmed its identity as T. melophagium. Most keds were found to harbour the trypanoso

APA, Harvard, Vancouver, ISO, and other styles

20

Allaby, Robin G., Monica Banerjee, and Terence A. Brown. "Evolution of the high molecular weight glutenin loci of the A, B, D, and G genomes of wheat." Genome 42, no. 2 (1999): 296–307. http://dx.doi.org/10.1139/g98-114.

Full text

Abstract:

We used PCR to obtain phylogenetically informative sequences from the high molecular weight glutenin genes of wheat. The validity of partial sequence comparisons as a means of studying glutenin phylogenetics was established by constructing neighbour-joining trees from partial alignments of 12 published glutenin allele sequences. PCR was then used to obtain 20 novel glutenin allele sequences from various Triticum and Aegilops species, including a 3000 year old preserved wheat. A neighbour-joining tree derived from all known glutenin allele sequences had eight clades, representing the eight loci

APA, Harvard, Vancouver, ISO, and other styles

21

Rourke, Ian J., Jens F. Rehfeld, Morten Møller, and Anders H. Johnsen. "Characterization of the Cholecystokinin and Gastrin Genes from the Bullfrog, Rana catesbeiana: Evolutionary Conservation of Primary and Secondary Sites of Gene Expression." Endocrinology 138, no. 4 (1997): 1719–27. http://dx.doi.org/10.1210/endo.138.4.5084.

Full text

Abstract:

Abstract The gastrin and cholecystokinin (CCK) genes, and the complementary DNAs they encode, have been isolated and sequenced from the bullfrog, Rana catesbeiana. The CCK gene promoter region possess the same four well characterized transcriptional control elements as the human CCK gene, namely an E-box, AP-1 binding site, Sp1 site, and TATA box. In contrast, no obvious regulatory motifs are conserved in the gastrin gene. Alignment of the bullfrog preprohormone sequences with other members of the CCK/gastrin peptide family showed that preproCCK has been conserved to a greater degree during ev

APA, Harvard, Vancouver, ISO, and other styles

22

Palena, A., A. Blau, G. Stamatoyannopoulos, and NP Anagnou. "Eastern European (delta beta) zero-thalassemia: molecular characterization of a novel 9.1-kb deletion resulting in high levels of fetal hemoglobin in the adult [see comments]." Blood 83, no. 12 (1994): 3738–45. http://dx.doi.org/10.1182/blood.v83.12.3738.3738.

Full text

Abstract:

Abstract A novel deletion in the human beta-globin gene cluster associated with increased levels of fetal hemoglobin (HbF) in adult life was molecularly characterized in a member of a family of Eastern European descent. The phenotype of the deletion, documented in five members of the family, shows mild hypochromia and microcytosis (mean corpuscular Hb, 24 to 25.9 pg; mean corpuscular volume, 74 to 78.5 fL) but high production of HbF (13% to 24%) with heterocellular distribution (36% to 86% F cells). Extensive restriction enzyme mapping of the beta-globin cluster and sequencing of the region en

APA, Harvard, Vancouver, ISO, and other styles

23

Palena, A., A. Blau, G. Stamatoyannopoulos, and NP Anagnou. "Eastern European (delta beta) zero-thalassemia: molecular characterization of a novel 9.1-kb deletion resulting in high levels of fetal hemoglobin in the adult [see comments]." Blood 83, no. 12 (1994): 3738–45. http://dx.doi.org/10.1182/blood.v83.12.3738.bloodjournal83123738.

Full text

Abstract:

A novel deletion in the human beta-globin gene cluster associated with increased levels of fetal hemoglobin (HbF) in adult life was molecularly characterized in a member of a family of Eastern European descent. The phenotype of the deletion, documented in five members of the family, shows mild hypochromia and microcytosis (mean corpuscular Hb, 24 to 25.9 pg; mean corpuscular volume, 74 to 78.5 fL) but high production of HbF (13% to 24%) with heterocellular distribution (36% to 86% F cells). Extensive restriction enzyme mapping of the beta-globin cluster and sequencing of the region encompassin

APA, Harvard, Vancouver, ISO, and other styles

24

Akafyi, Danlami Elisha, Iliya Shehu Ndams, Ishaya Haruna Nock, et al. "Emergence and genetic diversity of zoonotic Onchocerca species among human populations in Taraba State, Nigeria." German Journal of Microbiology 3, no. 2 (2023): 12–19. http://dx.doi.org/10.51585/gjm.2023.2.0023.

Full text

Abstract:

In the present study, the genetic diversity of Onchocerca (O.) species was investigated by amplifying a fragment of the 16S rRNA gene from the parasite obtained in some endemic areas in Taraba State, Nigeria. Three local government areas were selected: two onchocerciasis-endemic and one non-onchocerciasis-endemic regions. A total of 211 skin snips using a sterile sclera punch were obtained from consenting participants, males and females residing within the areas for at least ten years or since birth, by convenience sampling methods. The emerged microfilariae were examined microscopically. Nine

APA, Harvard, Vancouver, ISO, and other styles

25

Ibarguchi, Gabriela, Vicki L. Friesen, and Stephen C. Lougheed. "Defeating numts: Semi-pure mitochondrial DNA from eggs and simple purification methods for field-collected wildlife tissues." Genome 49, no. 11 (2006): 1438–50. http://dx.doi.org/10.1139/g06-107.

Full text

Abstract:

Mitochondrial DNA (mtDNA) continues to play a pivotal role in phylogeographic, phylogenetic, and population genetic studies. PCR amplification with mitochondrial primers often yields ambiguous sequences, in part because of the coamplification of nuclear copies of mitochondrial genes (numts) and true mitochondrial heteroplasmy arising from mutations, hybridization with paternal leakage, gene duplications, and recombination. Failing to detect numts or to distinguish the origin of such homologous sequences results in the incorrect interpretation of data. However, few studies obtain purified mtDNA

APA, Harvard, Vancouver, ISO, and other styles

26

Hsu, Wen-Kuang, Shih-Chieh Lee, and Pei-Luen Lu. "A Useful Technical Application of the Identification of Nucleotide Sequence Polymorphisms and Gene Resources for Cinnamomum osmophloeum Kaneh. (Lauraceae)." Forests 10, no. 4 (2019): 306. http://dx.doi.org/10.3390/f10040306.

Full text

Abstract:

The plant genus Cinnamomum contains economically important evergreen aromatic trees and shrubs belonging to the laurel family, Lauraceae. Our study tree species Cinnamomum osmophloeum Kaneh. (CO) has high economic value in Taiwan. The present study attempts to identify the gene resources of Cinnamomum osmophloeum Kaneh. by analyzing the nucleotide sequences of the partial noncoding internal transcribed spacer 2 (pITS2) of the ribosomal DNA and the trnL-trnF chloroplast genome. Seventy-three geographical strains of Cinnamomum osmophloeum, preserved in the Lien Hua-Chin Research Center of the Fo

APA, Harvard, Vancouver, ISO, and other styles

27

Vivien, Régis, Inge Werner, and Benoit J. D. Ferrari. "Simultaneous preservation of the DNA quality, the community composition and the density of freshwater oligochaetes for the development of genetically based biological indices." PeerJ 6 (December 5, 2018): e6050. http://dx.doi.org/10.7717/peerj.6050.

Full text

Abstract:

IntroductionOligochaetes are recognized as valuable bioindicators of sediment quality in streams and lakes. The development of an oligochaete index based on the identification of specimens using DNA barcodes requires a method for simultaneously preserving the DNA quality and information on the specimen density and oligochaete community composition. Absolute ethanol optimally preserves DNA but fixation of freshwater oligochaetes with this medium can cause disintegration and fragmentation of specimens. Here, we investigated the possibility to preserve oligochaete specimens in low-pH formalin and

APA, Harvard, Vancouver, ISO, and other styles

28

TOMASONI, SUSANNA, NADIA AZZOLLINI, FEDERICA CASIRAGHI, MAURIZIO C. CAPOGROSSI, GIUSEPPE REMUZZI, and ARIELA BENIGNI. "CTLA4Ig Gene Transfer Prolongs Survival and Induces Donor-Specific Tolerance in a Rat Renal Allograft." Journal of the American Society of Nephrology 11, no. 4 (2000): 747–52. http://dx.doi.org/10.1681/asn.v114747.

Full text

Abstract:

Abstract. Organ transplantation requires lifelong antirejection therapy, which carries the risk of infection and cancer. A revolutionary approach is to transduce the organ graft with immunomodulatory genes to render them tolerated with no need of systemic immunosuppression. Prolonged allograft survival was achieved by adenovirus-mediated transduction of the cold-preserved kidney with sequences encoding CTLA4Ig, a recombinant fusion protein that blocks T cell activation. Organ expression of the transgene was achieved associated with mild infiltration of mononuclear cells in the transfected kidn

APA, Harvard, Vancouver, ISO, and other styles

29

Miranda, Jorge, Lina Violet-Lozano, Samia Barrera, et al. "Candidatus Rickettsia colombianensi in ticks from reptiles in Córdoba, Colombia." Veterinary World 13, no. 9 (2020): 1764–70. http://dx.doi.org/10.14202/vetworld.2020.1764-1770.

Full text

Abstract:

Background and Aim: Wildlife animals are reservoirs of a large number of microorganisms pathogenic to humans, and ticks could be responsible for the transmission of these pathogens. Rickettsia spp. are the most prevalent pathogens found in ticks. This study was conducted to detect Rickettsia spp. in ticks collected from free-living and illegally trafficked reptiles from the Department of Córdoba, Colombia. Materials and Methods: During the period from October 2011 to July 2014, ticks belonging to the family Ixodidae were collected, preserved in 96% ethanol, identified using taxonomic keys, and

APA, Harvard, Vancouver, ISO, and other styles

30

Osman, N., C. A. Kozak, I. F. McKenzie, and P. M. Hogarth. "Structure and mapping of the gene encoding mouse high affinity Fc gamma RI and chromosomal location of the human Fc gamma RI gene." Journal of Immunology 148, no. 5 (1992): 1570–75. http://dx.doi.org/10.4049/jimmunol.148.5.1570.

Full text

Abstract:

Abstract We describe the isolation and characterization of the gene encoding the mouse high affinity Fc receptor Fc gamma RI. Using a mouse cDNA Fc gamma RI probe four unique overlapping genomic clones were isolated and were found to encode the entire 9 kb of the mouse Fc gamma RI gene. Sequence analysis of the gene showed that six exons account for the entire Fc gamma RI cDNA sequences including the 5'- and 3'-untranslated sequences. The first and second exons encode the signal peptide; exons 3, 4, and 5 encode the extracellular Ig binding domains; and exon 6 encodes the transmembrane domain,

APA, Harvard, Vancouver, ISO, and other styles

31

Xiong, Bai, and Thomas D. Kocher. "Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae)." Genome 34, no. 2 (1991): 306–11. http://dx.doi.org/10.1139/g91-050.

Full text

Abstract:

Universal primers constructed from the 16S ribosomal RNA gene in the Drosophila yakuba mitochondrial genome were successfully used to amplify, via the polymerase chain reaction, the homologous region of mitochondrial DNA from seven black fly morphospecies. Amplification was achieved from single larval salivary glands and from single adults preserved in Carnoy's fixative (ethanol – acetic acid, 3:1), allowing DNA sequences and polytene chromosome banding pattern data to be gathered from the same individuals. Nucleotide sequences of the amplified DNA segment (347 base pairs) were obtained from a

APA, Harvard, Vancouver, ISO, and other styles

32

Southey, Bruce R., and Sandra L. Rodriguez-Zas. "Changes in Neuropeptide Prohormone Genes among Cetartiodactyla Livestock and Wild Species Associated with Evolution and Domestication." Veterinary Sciences 9, no. 5 (2022): 247. http://dx.doi.org/10.3390/vetsci9050247.

Full text

Abstract:

The impact of evolution and domestication processes on the sequences of neuropeptide prohormone genes that participate in cell–cell signaling influences multiple biological process that involve neuropeptide signaling. This information is important to understand the physiological differences between Cetartiodactyla domesticated species such as cow, pig, and llama and wild species such as hippopotamus, giraffes, and whales. Systematic analysis of changes associated with evolutionary and domestication forces in neuropeptide prohormone protein sequences that are processed into neuropeptides was un

APA, Harvard, Vancouver, ISO, and other styles

33

Bik, Holly M., Lawrence E. Hawkins, J. Alan Hughes, and John D. Lambshead. "Rapid decline of PCR amplification from genomic extracts of DESS-preserved, slide-mounted nematodes." Nematology 11, no. 6 (2009): 827–34. http://dx.doi.org/10.1163/156854109x422922.

Full text

Abstract:

Abstract Many studies use integrative methods to study both morphology and gene sequences of nematode species, yet there is little evidence to indicate the optimum criteria for merging taxonomic and molecular protocols. Preliminary evidence suggests that standard methods of desiccation and slide mounting nematode specimens in glycerin can sporadically result in degradation of genomic DNA. A time series experiment was constructed in order to assess whether this degradation of genomic DNA could be recorded and quantified. Two groups of nematode specimens were desiccated, mounted on slides, and s

APA, Harvard, Vancouver, ISO, and other styles

34

Rojas, Ana Maria, Gloria Fuentes, Antonio Rausell, and Alfonso Valencia. "The Ras protein superfamily: Evolutionary tree and role of conserved amino acids." Journal of Cell Biology 196, no. 2 (2012): 189–201. http://dx.doi.org/10.1083/jcb.201103008.

Full text

Abstract:

The Ras superfamily is a fascinating example of functional diversification in the context of a preserved structural framework and a prototypic GTP binding site. Thanks to the availability of complete genome sequences of species representing important evolutionary branch points, we have analyzed the composition and organization of this superfamily at a greater level than was previously possible. Phylogenetic analysis of gene families at the organism and sequence level revealed complex relationships between the evolution of this protein superfamily sequence and the acquisition of distinct cellul

APA, Harvard, Vancouver, ISO, and other styles

35

Bogomaz, Olesja D., Victoria D. Bemova, Nikita A. Mirgorodskii, and Tatiana V. Matveeva. "Evolutionary Fate of the Opine Synthesis Genes in the Arachis L. Genomes." Biology 13, no. 8 (2024): 601. http://dx.doi.org/10.3390/biology13080601.

Full text

Abstract:

Naturally transgenic plants are plants that have undergone Agrobacterium-mediated transformation under natural conditions without human involvement. Among Arachis hypogaea L., A. duranensis Krapov. & W.C. Greg, A. ipaensis Krapov. & W.C. Greg, A. monticola Krapov. & Rigoni, and A. stenosperma Krapov. & W.C. Greg are known to contain sequences derived from the T-DNA of “Agrobacterium”. In the present study, using molecular genetics and bioinformatic methods, we characterized natural transgenes in 18 new species from six sections of the genus Arachis. We found that small fragment

APA, Harvard, Vancouver, ISO, and other styles

36

Pancaningtyas, Sulistyani, Ariza Budi Tunjung Sari, and Dwi Suci Rahayu. "DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene." Pelita Perkebunan (a Coffee and Cocoa Research Journal) 38, no. 3 (2022): 179–89. http://dx.doi.org/10.22302/iccri.jur.pelitaperkebunan.v38i3.521.

Full text

Abstract:

   Helopeltis sp. is one of the major pests of the cocoa plant. Helopeltis can decrease cocoa yield by 50 to 60%. Several species of Helopeltis spp. are still often misidentified due to similarity in morphology such as size and color. This study aimed to identify Helopeltis sp. through DNA barcoding using the mitochondrial cyto- chrome C oxidase subunit 1 (COI) gene. Analysis was carried out on 100 adults Helopeltis sp. from Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. Specimens were preserved in 96% ethyl alcohol

APA, Harvard, Vancouver, ISO, and other styles

37

Parenti, Lynne R., Diane E. Pitassy, Zeehan Jaafar, Kirill Vinnikov, Niamh E. Redmond, and Kathleen S. Cole. "Fishes collected during the 2017 MarineGEO assessment of Kāne‘ohe Bay, O‘ahu, Hawai‘i." Journal of the Marine Biological Association of the United Kingdom 100, no. 4 (2020): 607–37. http://dx.doi.org/10.1017/s0025315420000417.

Full text

Abstract:

AbstractWe report the results of a survey of the fishes of Kāne‘ohe Bay, O‘ahu, conducted in 2017 as part of the Smithsonian Institution MarineGEO Hawaii bioassessment. We recorded 109 species in 43 families. The most speciose families were Acanthuridae (11 species), Gobiidae (11 species), Pomacentridae (10) and Chaetodontidae (9 species). Nine of the species that we collected are known or suspected to be introduced to the Hawaiian Islands. Specimens were identified, measured and photographed. All specimen vouchers were fixed in formalin and ultimately transferred to 75% ethanol for long-term

APA, Harvard, Vancouver, ISO, and other styles

38

Jarzembowski, P., H. Berniak, A. Faltyn, A. Jakubska-Busse, and J. Proćków. "First Report of ‘Candidatus Phytoplasma asteris’ Associated with “Witches'-Brooms” on Jointleaf Rush (Juncus articulatus) in Poland." Plant Disease 99, no. 2 (2015): 281. http://dx.doi.org/10.1094/pdis-06-14-0614-pdn.

Full text

Abstract:

Juncus articulatus L. (Juncaceae) is a species of rush occurring in Eurasia, Canada, and the United States. In 2013, symptoms of “witches'-brooms,” similar to those associated with phytoplasma infection in other plants, were observed on jointleaf rush plants in Lower Silesia (southwest Poland), with some pests feeding on them. Livia junci (Liviinae, Hemiptera) is a small plant-feeding sap-sucking insect that affects monocotyledonous plants. To confirm the presence of phytoplasma in 15 examined plants, total DNA was extracted from 100 mg of inflorescence and leaf samples collected in July 2013

APA, Harvard, Vancouver, ISO, and other styles

39

Horie, Masayuki, Yuki Kobayashi, Tomoyuki Honda, et al. "An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus." Scientific Reports 6, no. 1 (2016): 25873. https://doi.org/10.5281/zenodo.13446864.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Abstract Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analys

APA, Harvard, Vancouver, ISO, and other styles

40

Horie, Masayuki, Yuki Kobayashi, Tomoyuki Honda, et al. "An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus." Scientific Reports 6, no. 1 (2016): 25873. https://doi.org/10.5281/zenodo.13446864.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Abstract Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analys

APA, Harvard, Vancouver, ISO, and other styles

41

Horie, Masayuki, Yuki Kobayashi, Tomoyuki Honda, et al. "An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus." Scientific Reports 6, no. 1 (2016): 25873. https://doi.org/10.5281/zenodo.13446864.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Abstract Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analys

APA, Harvard, Vancouver, ISO, and other styles

42

Horie, Masayuki, Yuki Kobayashi, Tomoyuki Honda, et al. "An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus." Scientific Reports 6, no. 1 (2016): 25873. https://doi.org/10.5281/zenodo.13446864.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Abstract Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analys

APA, Harvard, Vancouver, ISO, and other styles

43

Horie, Masayuki, Yuki Kobayashi, Tomoyuki Honda, et al. "An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus." Scientific Reports 6, no. 1 (2016): 25873. https://doi.org/10.5281/zenodo.13446864.

Full text

Abstract:

(Uploaded by Plazi for the Bat Literature Project) Abstract Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11.8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1,718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analys

APA, Harvard, Vancouver, ISO, and other styles

44

Florea, S., C. Machado, D. Zhang, D. G. Panaccione, and C. L. Schardl. "Towards the elimination of ergot alkaloid biosynthesis genes in Neotyphodium Coenophialum." NZGA: Research and Practice Series 13 (January 1, 2007): 477–79. http://dx.doi.org/10.33584/rps.13.2006.3127.

Full text

Abstract:

Neotyphodium coenophialum strain e19 from tall fescue cv. Kentucky 31 carries dmaW1 and dmaW2, two gene homologues that encode dimethylallyltryptophan synthase, the enzyme for the first step in ergot-alkaloid biosynthesis. In our effort to disrupt both homologues and ultimately obtain marker-free mutants, we are using a marker-exchange strategy employing the Cre/ loxP site-specific recombination system. Of 1522 transformants obtained and screened, three were likely dmaW2 disruptants because they gave no PCR product from the wild-type locus, but yielded the larger PCR fragment from the disrupti

APA, Harvard, Vancouver, ISO, and other styles

45

Arndt, Thomas, and Michael Wink. "Molecular Systematics, Taxonomy and Distribution of the Pyrrhura Picta–Leucotis Complex." Open Ornithology Journal 10, no. 1 (2017): 53–91. http://dx.doi.org/10.2174/1874453201710010053.

Full text

Abstract:

Introduction: The relationships within the Pyrrhura species complex are partly unresolved. In this study, a comprehensive phylogenetic analysis of the Pyrrhura picta-leucotis complex was carried out, covering all species except P. subandina. Material and Methods: We made a morphological analysis of 745 preserved specimens of all the taxa in different museums. Nucleotide sequences of the mitochondrial cytochrome gene were generated and used to reconstruct a molecular phylogeny of Pyrrhura. Results and Discussion: Our results show that the complex is divided into 6 main groups comprising 15 spec

APA, Harvard, Vancouver, ISO, and other styles

46

Trevisan, Bruna, Daniel M. C. Alcantara, Denis Jacob Machado, Fernando P. L. Marques, and Daniel J. G. Lahr. "Genome skimming is a low-cost and robust strategy to assemble complete mitochondrial genomes from ethanol preserved specimens in biodiversity studies." PeerJ 7 (September 13, 2019): e7543. http://dx.doi.org/10.7717/peerj.7543.

Full text

Abstract:

Global loss of biodiversity is an ongoing process that concerns both local and global authorities. Studies of biodiversity mainly involve traditional methods using morphological characters and molecular protocols. However, conventional methods are a time consuming and resource demanding task. The development of high-throughput sequencing (HTS) techniques has reshaped the way we explore biodiversity and opened a path to new questions and novel empirical approaches. With the emergence of HTS, sequencing the complete mitochondrial genome became more accessible, and the number of genome sequences

APA, Harvard, Vancouver, ISO, and other styles

47

Zhenilo, S. V., A. S. Sokolov, and E. B. Prokhortchou. "Epigenetics of Ancient DNA." Acta Naturae 8, no. 3 (2016): 72–76. http://dx.doi.org/10.32607/20758251-2016-8-3-72-76.

Full text

Abstract:

Initially, the study of DNA isolated from ancient specimens had been based on the analysis of the primary nucleotide sequence. This approach has allowed researchers to study the evolutionary changes that occur in different populations and determine the influence of the environment on genetic selection. However, the improvement of methodological approaches to genome-wide analysis has opened up new possibilities in the search for the epigenetic mechanisms involved in the regulation of gene expression. It was discovered recently that the methylation status of the regulatory elements of the HOXD c

APA, Harvard, Vancouver, ISO, and other styles

48

He, Jing, David M. Irwin, Rui Chen, and Ya-Ping Zhang. "Stepwise loss of motilin and its specific receptor genes in rodents." Journal of Molecular Endocrinology 44, no. 1 (2009): 37–44. http://dx.doi.org/10.1677/jme-09-0095.

Full text

Abstract:

Specific interactions among biomolecules drive virtually all cellular functions and underlie phenotypic complexity and diversity. Biomolecules are not isolated particles, but are elements of integrated interaction networks, and play their roles through specific interactions. Simultaneous emergence or loss of multiple interacting partners is unlikely. If one of the interacting partners is lost, then what are the evolutionary consequences for the retained partner? Taking advantages of the availability of the large number of mammalian genome sequences and knowledge of phylogenetic relationships o

APA, Harvard, Vancouver, ISO, and other styles

49

Frischer, Marc E., Peter J. Floriani, and Sandra A. Nierzwicki-Bauer. "Differential sensitivity of 16S rRNA targeted oligonucleotide probes used for fluorescence in situ hybridization is a result of ribosomal higher order structure." Canadian Journal of Microbiology 42, no. 10 (1996): 1061–71. http://dx.doi.org/10.1139/m96-136.

Full text

Abstract:

The use of 16S rRNA targeted gene probes for the direct analysis of microbial communities has revolutionized the field of microbial ecology, yet a comprehensive approach for the design of such probes does not exist. The development of 16S rRNA targeted oligonucleotide probes for use with fluorescence in situ hybridization (FISH) procedures has been especially difficult as a result of the complex nature of the rRNA target molecule. In this study a systematic comparison of 16S rRNA targeted oligonucleotide gene probes was conducted to determine if target location influences the hybridization eff

APA, Harvard, Vancouver, ISO, and other styles

50

Nogami, M., A. Kohda, H. Taguchi, M. Nakao, T. Ikemura, and K. Okumura. "Relative locations of the centromere and imprinted SNRPN gene within chromosome 15 territories during the cell cycle in HL60 cells." Journal of Cell Science 113, no. 12 (2000): 2157–65. http://dx.doi.org/10.1242/jcs.113.12.2157.

Full text

Abstract:

Investigations of imprinted regions provide clues that increase our understanding of the regulation of gene functions at higher order chromosomal domains. Here, the relative positions of the chromosome 15 centromere and the imprinted SNRPN gene in interphase nuclei of human myeloid leukemia HL60 cells were compared, because the homologous association of this imprinted chromosomal domain was previously observed in lymphocytes and lymphoblasts. Four targets including the chromosome 15 territory, its centromere, the SNRPN gene on this chromosome, and the nucleus, were visualized simultaneously in

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!