Relevant bibliographies by topics / Primordial germ cells (PGCs)

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Primordial germ cells (PGCs)'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "Primordial germ cells (PGCs)"

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Xin-Yan, Tang, Zeng Wei-Dong, Mi Yu-Ling, Liu Hong-Yun, and Zhang Cai-Qiao. "Isolation, culture and characterization of chicken primordial germ cells." Chinese Journal of Agricultural Biotechnology 3, no. 3 (2006): 183–88. http://dx.doi.org/10.1079/cjb2006107.

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AbstractPrimordial germ cells (PGCs) were isolated from the genital ridges of chicken (Gallus domesticus) embryos at the 19th stage and purified by Ficoll density-gradient centrifugation. PGCs were co-cultured with somatic cells in preliminary culture and subcultured. Identification of PGCs was carried out by histochemical methods, including alkaline phosphatase (AKP) and periodic acid–Schiff (PAS). The proliferating activity of PGCs in subculture was demonstrated by immunocytochemistry of proliferating cell nuclear antigen (PCNA). Meanwhile, proliferating PGCs were compared under different cu
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Stott, D., and C. C. Wylie. "Invasive behaviour of mouse primordial germ cells in vitro." Journal of Cell Science 86, no. 1 (1986): 133–44. http://dx.doi.org/10.1242/jcs.86.1.133.

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We have isolated migrating primordial germ cells (PGCs) from 10.5-day mouse embryos and studied their behaviour when cultured on a mouse embryo fibroblast (STO) cell line. Living and fixed PGCs were identified by fluorescent labelling with a monoclonal antibody specific for PGCs in the culture system used. The behaviour of the cells was studied using interference reflexion microscopy (IRM) and time-lapse video cinematography. The IRM pattern displayed by PGCs is typical of highly motile cell types, the cells lack focal contacts and possess large areas of close contacts indicative of weak membr
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De Felici, Massimo. "Nuclear Reprogramming in Mouse Primordial Germ Cells: Epigenetic Contribution." Stem Cells International 2011 (2011): 1–15. http://dx.doi.org/10.4061/2011/425863.

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The unique capability of germ cells to give rise to a new organism, allowing the transmission of primary genetic information from generation to generation, depends on their epigenetic reprogramming ability and underlying genomic totipotency. Recent studies have shown that genome-wide epigenetic modifications, referred to as “epigenetic reprogramming”, occur during the development of the gamete precursors termed primordial germ cells (PGCs) in the embryo. This reprogramming is likely to be critical for the germ line development itself and necessary to erase the parental imprinting and setting t
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Hayashi, Yohei, Kei Otsuka, Masayuki Ebina, et al. "Distinct requirements for energy metabolism in mouse primordial germ cells and their reprogramming to embryonic germ cells." Proceedings of the National Academy of Sciences 114, no. 31 (2017): 8289–94. http://dx.doi.org/10.1073/pnas.1620915114.

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Primordial germ cells (PGCs), undifferentiated embryonic germ cells, are the only cells that have the ability to become gametes and to reacquire totipotency upon fertilization. It is generally understood that the development of PGCs proceeds through the expression of germ cell-specific transcription factors and characteristic epigenomic changes. However, little is known about the properties of PGCs at the metabolite and protein levels, which are directly responsible for the control of cell function. Here, we report the distinct energy metabolism of PGCs compared with that of embryonic stem cel
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Park, Eunsook, Bobae Lee, Bruce E. Clurman, and Keesook Lee. "NUP50 is necessary for the survival of primordial germ cells in mouse embryos." REPRODUCTION 151, no. 1 (2016): 51–58. http://dx.doi.org/10.1530/rep-14-0649.

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Nucleoporin 50 kDa (NUP50), a component of the nuclear pore complex, is highly expressed in male germ cells, but its role in germ cells is largely unknown. In this study, we analyzed the expression and function of NUP50 during the embryonic development of germ cells using NUP50-deficient mice. NUP50 was expressed in germ cells of both sexes at embryonic day 15.5 (E15.5), E13.5, and E12.5. In addition, NUP50 expression was also detected in primordial germ cells (PGCs) migrating into the genital ridges at E9.5. The gonads of Nup50−/− embryos of both sexes contained few PGCs at both E11.5 and E12
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Güralp, H., K. Pocherniaieva, M. Blecha, T. Policar, M. Pšenička, and T. Saito. "Migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation." Czech Journal of Animal Science 62, No. 3 (2017): 121–29. http://dx.doi.org/10.17221/40/2016-cjas.

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Pikeperch Sander lucioperca is a valuable fish in Europe, and basic information about its embryonic development, especially primordial germ cell (PGC) migration, is important for use in biotechnology. We categorized pikeperch embryonic development into six stages as in other fish species: zygote, cleavage, blastula, gastrula, segmentation, and hatching and described PGC migration. PGCs were visualized by injection of synthesized green fluorescent protein (GFP) within the 3’untranslated region (UTR) mRNA of nanos3. GFP-positive PGCs appeared in all embryos at approximately 100% epiboly. Time-la
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Higaki, Shogo, Yoshiki Eto, Yutaka Kawakami, et al. "Production of fertile zebrafish (Danio rerio) possessing germ cells (gametes) originated from primordial germ cells recovered from vitrified embryos." REPRODUCTION 139, no. 4 (2010): 733–40. http://dx.doi.org/10.1530/rep-09-0549.

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This study aimed to produce fertile zebrafish (Danio rerio) possessing germ cells (gametes) that originated from cryopreserved primordial germ cells (PGCs). First, to improve the vitrification procedure of PGCs in segmentation stage embryos, dechorionated yolk-intact and yolk-removed embryos, the PGCs of which were labeled with green fluorescent protein, were cooled rapidly after serial exposures to equilibration solution (ES) and vitrification solution (VS), which contained ethylene glycol, DMSO, and sucrose. Yolk removal well prevented ice formation in the embryos during cooling and improved
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Akita, Yasuko, and Masami Wakahara. "Cytological analyses of factors which determine the number of primordial germ cells (PGCs) in Xenopus laevis." Development 90, no. 1 (1985): 251–65. http://dx.doi.org/10.1242/dev.90.1.251.

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Correlation of the number of primordial germ cells (PGCs) at stage 47 with the amount of germ plasm at the 8-cell stage and with the number of the germ-plasm-containing cells (GPCCs) was analysed using two different laboratory-raised colonies of Xenopus laevis, HD and J groups. The average number of PGCs in J group tadpoles was significantly larger than that in HD group tadpoles. The amount of germ plasm in J group embryos was also demonstrated to be larger than in HD group embryos. The amount of germ plasm was related positively to the number of GPCCs at the 8-cell stage and to the resulting
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Chojnacka-Puchta, L., K. Kasperczyk, G. Płucienniczak, D. Sawicka, and M. Bednarczyk. "Primordial germ cells (PGCs) as a tool for creating transgenic chickens." Polish Journal of Veterinary Sciences 15, no. 1 (2012): 181–88. http://dx.doi.org/10.2478/v10181-011-0132-6.

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Primordial germ cells (PGCs) as a tool for creating transgenic chickens The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived f
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Soo Kang, Kyung, Hyung Chul Lee, Hyun Jeong Kim, et al. "Spatial and temporal action of chicken primordial germ cells during initial migration." REPRODUCTION 149, no. 2 (2015): 179–87. http://dx.doi.org/10.1530/rep-14-0433.

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In most animals, primordial germ cells (PGCs) originate from an extragonadal region and migrate across the embryo to the gonads, where they differentiate and function. During their migration, PGCs move passively by morphogenetic movement of the embryo or move actively through signaling molecules. To uncover the underlying mechanism of first-phase PGC migration toward the germinal crescent in chickens, we investigated the spatial and temporal action of PGCs during primitive streak formation. Exogenously transplanted PGCs migrated toward the anterior region of the embryo and the embryonic gonads
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Dissertations / Theses on the topic "Primordial germ cells (PGCs)"

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Souza, Aline Fernanda de. "Epigenética da reprogramação em células germinativas embrionárias caninas." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-24042017-172715/.

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As células germinativas primordiais (CGPs) são as precursoras dos gametas, capazes de gerar um novo indivíduo os quais transmitirão os materiais genéticos para as futuras gerações. Normalmente, a linha germinal de mamífero é determinada por fatores genéticos e epigenéticos que possuem funções essenciais para guiar na direção e desenvolvimento das CGPs, bem como das células germinativas embrionárias (CGEs). A reprogramação epigenética é fundamental para a regulação do genoma durante o desenvolvimento das células germinativas responsáveis por originar a linhagem gametogênica nos mamíferos. A met
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Nakaki, Fumio. "Induction of mouse germ-cell fate by transcription factors in vitro." Kyoto University, 2014. http://hdl.handle.net/2433/188684.

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Al-Thani, Rawda. "Primordial germ cells of the chick embryo." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315524.

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Li, Ying. "Transgenic birds from transformed primordial germ cells." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385118.

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Yoon, Christina Migyung 1970. "Idenficiation of the zebrafish primordial germ cells." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/43551.

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Wigmore, Kip. "The primordial germ cells of the goat fetus." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ43237.pdf.

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Seisenberger, Stefanie. "Reprogramming the epigenome of mouse primordial germ cells." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610019.

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Vick, Lorraine Mary. "Genetic manipulation of fowl via primordial germ cells." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317960.

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Pogool, Satian. "Factors controlling migration of avian primordial germ cells." Thesis, University of Manchester, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531435.

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Avian embryos have played an important role in the study of vertebrate development but further understanding of their development may also benefit the commercial poultry industry. Manipulation and use of primordial germ cells (PGCs) as a vehicle for constructed genotypes will be increasingly important for future improvement of commercial poultry including the turkey. However, the manipulation and use of PGCs will depend on a basic understanding of PGC migration. PGCs are known to migrate considerable distances before colonising the gonad. Previous work in the chick and quail have suggested tha
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Grabole, Nils. "Functional analysis of Prdm14 in primordial germ cells and stem cells." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608092.

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Book chapters on the topic "Primordial germ cells (PGCs)"

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Labosky, Patricia A., and Brigid L. M. Hogan. "Mouse Primordial Germ Cells." In METHODS IN MOLECULAR BIOLOGY™. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-483-8_12.

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Tagami, Takahiro, Daichi Miyahara, and Yoshiaki Nakamura. "Avian Primordial Germ Cells." In Advances in Experimental Medicine and Biology. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3975-1_1.

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Wu, Ji, Zhuxia Zheng, Hu Wang, Xingxing Mei, Xingbao Ding, and Xiaoyong Li. "Primordial Germ Cells and Germ Line Stem Cells." In Translational Medicine Research. Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-017-7273-0_1.

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McLaren, Anne. "Primordial Germ Cells in Mammals." In Organization of the Early Vertebrate Embryo. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4899-1618-1_1.

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Motono, Makoto, Hiroyuki Komatsu, Yoshinori Kawabe, Ken-Ichi Nishijima, and Shinji Iijima. "Sterilization of Chicken Primordial Germ Cells." In Animal Cell Technology: Basic & Applied Aspects. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-9646-4_26.

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Smith, Emily Y., and James L. Resnick. "Immunomagnetic Purification of Murine Primordial Germ Cells." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-011-3_4.

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Hayashi, Yohei, and Yasuhisa Matsui. "Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells." In Stem Cells and Aging. Springer New York, 2018. http://dx.doi.org/10.1007/7651_2018_164.

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Sarkar, Saumya, and Rajender Singh. "Primordial germ cells: Origin, migration and testicular development." In Molecular Signaling in Spermatogenesis and Male Infertility. CRC Press, 2019. http://dx.doi.org/10.1201/9780429244216-1.

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Aflatoonian, Behrouz, and Harry D. Moore. "Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells." In Springer Protocols Handbooks. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-267-0_7.

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Labosky, Patricia A., Denise P. Barlow, and Brigid L. M. Hogan. "Embryonic Germ Cell Lines and Their Derivation from Mouse Primordial Germ Cells." In Ciba Foundation Symposium 182 - Germline Development. John Wiley & Sons, Ltd., 2007. http://dx.doi.org/10.1002/9780470514573.ch9.

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Conference papers on the topic "Primordial germ cells (PGCs)"

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Soni, Abhishek, Nupur Bansal, A. K. Dhull, Vivek Kaushal, and A. K. Chauhan. "Pure primary non gestational choriocarcinoma ovary – diagnostic dilemma and treatment intricacy." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685331.

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Introduction: Germ cell tumors of the ovary include all neoplasm derived from primordial germ cells of the embryonal gonad. Five percent of germ cell tumors are malignant, representing three to five per cent of all ovarian carcinomas of which pure primary non-gestational ovarian choriocarcinoma accounts for less than one per cent of ovarian tumors. Primary choriocarcinoma of ovary could be gestational or nongestational in origin. They pose diagnostic challenges in reproductive age group patients because of elevated human chorionic gonadotrophin (hCG). Non-gestational choriocarcinoma (NGCO) is
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