Log in

Relevant bibliographies by topics / Primordial germ cells (PGCs) / Dissertations / Theses

To see the other types of publications on this topic, follow the link: Primordial germ cells (PGCs).

Dissertations / Theses on the topic 'Primordial germ cells (PGCs)'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 dissertations / theses for your research on the topic 'Primordial germ cells (PGCs).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Souza, Aline Fernanda de. "Epigenética da reprogramação em células germinativas embrionárias caninas." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-24042017-172715/.

Full text

Abstract:

As células germinativas primordiais (CGPs) são as precursoras dos gametas, capazes de gerar um novo indivíduo os quais transmitirão os materiais genéticos para as futuras gerações. Normalmente, a linha germinal de mamífero é determinada por fatores genéticos e epigenéticos que possuem funções essenciais para guiar na direção e desenvolvimento das CGPs, bem como das células germinativas embrionárias (CGEs). A reprogramação epigenética é fundamental para a regulação do genoma durante o desenvolvimento das células germinativas responsáveis por originar a linhagem gametogênica nos mamíferos. A met

APA, Harvard, Vancouver, ISO, and other styles

2

Nakaki, Fumio. "Induction of mouse germ-cell fate by transcription factors in vitro." Kyoto University, 2014. http://hdl.handle.net/2433/188684.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Al-Thani, Rawda. "Primordial germ cells of the chick embryo." Thesis, University of Reading, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315524.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Li, Ying. "Transgenic birds from transformed primordial germ cells." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385118.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Yoon, Christina Migyung 1970. "Idenficiation of the zebrafish primordial germ cells." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/43551.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Wigmore, Kip. "The primordial germ cells of the goat fetus." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ43237.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Seisenberger, Stefanie. "Reprogramming the epigenome of mouse primordial germ cells." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610019.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Vick, Lorraine Mary. "Genetic manipulation of fowl via primordial germ cells." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317960.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Pogool, Satian. "Factors controlling migration of avian primordial germ cells." Thesis, University of Manchester, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531435.

Full text

Abstract:

Avian embryos have played an important role in the study of vertebrate development but further understanding of their development may also benefit the commercial poultry industry. Manipulation and use of primordial germ cells (PGCs) as a vehicle for constructed genotypes will be increasingly important for future improvement of commercial poultry including the turkey. However, the manipulation and use of PGCs will depend on a basic understanding of PGC migration. PGCs are known to migrate considerable distances before colonising the gonad. Previous work in the chick and quail have suggested tha

APA, Harvard, Vancouver, ISO, and other styles

10

Grabole, Nils. "Functional analysis of Prdm14 in primordial germ cells and stem cells." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608092.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Petyim, Somsim. "Gene regulation during development of human primordial germ cells." Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.665476.

Full text

Abstract:

A critical event during embryonic development is the segregation of the germ line from the soma. The germ line founders, or primordial germ cells (PO C), are the precursors of the gametes, which enable the transmission of the genetic material to future generations. In rodents, the PGC precursors are segregated from somatic lineages before the start of gastrulation. In several model organisms segregation of the germ line is accomplished by maternally inherited transcriptional repressors contained in the egg's germ plasm. In mammals, PGC specification is regulated by epigenesis, a mechanism by w

APA, Harvard, Vancouver, ISO, and other styles

12

Intarapat, S. "Isolation and characterisation of chick embryonic primordial germ cells." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1344049/.

Full text

Abstract:

Embryonic stem cells (cESCs) can be isolated from chick embryos, with the ability to contribute to all somatic lineages in chimaeras, but not to the germ line. However, lines of chicken embryonic germ cells (cEGCs), which are able to contribute to the germ line, can be established from chicken primordial germ cells (cPGCs). However very little is known about these cells, or about the changes that accompany the establishment of gonadal cells as self-renewing cell lines. This thesis presents a detailed study of the properties of cPGCs and the parent tissue from which they are derived. Gene expre

APA, Harvard, Vancouver, ISO, and other styles

13

Garcia-Castro, Martin Ignacio. "Interaction between migrating mouse primordial germ cells and extracellular matrix molecules." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389802.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Zhang, Haixin. "Gene regulatory network and epigenetic reprogramming of pig primordial germ cells." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33452/.

Full text

Abstract:

Primordial germ cells (PGC) are the precursors of the gametes. The mechanisms of PGC induction, specification and development are very well characterized in rodents, however recent investigations have demonstrated that the mechanisms of germ cell development differ significantly between mice and humans. Since the knowledge of PGC development in non-rodents is very limited, and early human embryos cannot be accessed it is important to establish a new model for PGC development with relevance to humans. In this thesis, I use pig embryo as a model for investigating PGC development in non-rodent ma

APA, Harvard, Vancouver, ISO, and other styles

15

STALLOCK, JAMES PATRICK. "THE ROLE OF BAX IN APOPTOSIS OF ECTOPIC PRIMORDIAL GERM CELLS IN THE MOUSE." University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1046891667.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Erhardt, Sylvia. "Epigenetic changes during development of murine primordial germ cells and early embryos." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619772.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

McCormick, Rachel Jacqueline. "Investigations into Xpat, a novel gene expressed in the germ plasm and primordial germ cells of Xenopus laevis." Thesis, University of Warwick, 2001. http://wrap.warwick.ac.uk/3093/.

Full text

Abstract:

To determine the expression pattern of XPAT (Xenopus primordial germ cell associated transcript) protein in Xenopus oocytes, XPAT-GFP fusion proteins were generated. When XPAT was amino-terminally tagged with GFP it became localised to the nuclei of stage VI Xenopus oocytes. However, when carboxy-terminally tagged with GFP, XPAT also translocated to the vegetal pole of stage VI oocytes. XPAT-GFP formed particles (1 to 2.5mm in diameter) which aggregated into large (10 to 50mm) granular structures at the vegetal pole. These particles looked exactly like those seen after in situ hybridisation to

APA, Harvard, Vancouver, ISO, and other styles

18

Adhikari, Deepak. "Signaling pathways in the development of female germ cells." Doctoral thesis, Umeå universitet, Institutionen för medicinsk kemi och biofysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-88309.

Full text

Abstract:

Primordial follicles are the first small follicles to appear in the mammalian ovary. Women are born with a fixed number of primordial follicles in the ovaries. Once formed, the pool of primordial follicles serves as a source of developing follicles and oocytes. The first aim of this thesis was to investigate the functional role of the intra-oocyte signaling pathways, especially the phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin complex 1 (mTORC1) pathways in the regulation of primordial follicle activation and survival. We found that a primordial follicle remains dorman

APA, Harvard, Vancouver, ISO, and other styles

19

Macdonald, Joni. "In vitro culture and transposon-mediated genetic modification of chicken primordial germ cells." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/6498.

Full text

Abstract:

Primordial germ cells (PGCs) are the embryonic precursors of the germ cell lineage. Segregation of the chicken germ line from somatic cells occurs very early in embryonic development. By day two of incubation chicken PGCs can be isolated from the circulating blood. The in vitro culture of chicken PGCs has significant potential as a tool for the investigation of germ cell development and as a cell-based system for the production of genetically modified chickens. The isolation, culture and manipulation of migratory chicken PGCs reported previously have not been independently validated. Initial a

APA, Harvard, Vancouver, ISO, and other styles

20

Yamaguchi, Shinpei. "Conditional Knockdown of Nanog Induces Apoptotic Cell Death in Mouse Migrating Primordial Germ Cells." Kyoto University, 2010. http://hdl.handle.net/2433/97931.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Murase, Yusuke. "Long-term expansion with germline potential of human primordial germ cell-like cells in vitro." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/261607.

Full text

Abstract:

京都大学<br>0048<br>新制・課程博士<br>博士(医学)<br>甲第22880号<br>医博第4674号<br>新制||医||1047(附属図書館)<br>京都大学大学院医学研究科医学専攻<br>(主査)教授篠原隆司, 教授近藤玄, 教授万代昌紀<br>学位規則第4条第1項該当<br>Doctor of Medical Science<br>Kyoto University<br>DFAM

APA, Harvard, Vancouver, ISO, and other styles

22

Yamada, Yukiko. "Deciphering molecular mechanisms that regulate programmed cell death of primordial germ cells in Drosophila melanogaster." [Ames, Iowa : Iowa State University], 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3337369.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Stunden, Carolyn. "Selection for primordial germ cells in the stage X chick embryo using magnetic-activated cell sorting." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ43274.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Gu, Ying. "A Traveling Niche: The Role of Steel Factor in Mouse Primordial Germ Cell Development." University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1321370449.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Glover, James David. "Investigating the role of PRDM14 in the avian germ cell lineage using a novel inducible DNA transposon system." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/18008.

Full text

Abstract:

Primordial germ cells (PGCs) are the precursors of the germ cell lineage that eventually differentiate into mature spermatozoa and oocytes. Although present throughout the animal kingdom, the specification and migration of PGCs differs widely between species. In vertebrates, avians are evolutionary divergent from mammals and therefore allow a comparative system in which to study germ cell development in higher organisms. Unlike mouse, PGCs can be isolated from the chicken embryo, expanded and cultured long term in vitro. Analysis of these cells showed that cultured chicken PGCs maintain the ch

APA, Harvard, Vancouver, ISO, and other styles

26

Wade, Alexander James. "Turkey (Meleagris gallopavo) primordial germ cells and the potential for using embryonic chimeras to safeguard commercial turkey strains." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.495050.

Full text

Abstract:

Diseases such as avian influenza (Al) virus can result in the destruction of whole chicken and turkey flocks. Many valuable and rare strains of poultry are maintained on farms worldwide, making them particularly susceptible to outbreaks of such diseases and resulting in a loss of valuable genetic material from the turkey gene pool, with consequences for the poultry Industry. Primordial germ cells (PGCs) are the stem cell precursors to the gametes and they take a complex migratory route throughout the developing avian embryo. starting from the epiblast and moving to the germinal crescent, blood

APA, Harvard, Vancouver, ISO, and other styles

27

Yamashiro, Chika. "Generation of human oogonia from induced pluripotent stem cells in vitro." Kyoto University, 2019. http://hdl.handle.net/2433/242826.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Ahmed, Manal Bayomi Mahmoud. "The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells." [S.l.] : [s.n.], 2001. http://webdoc.sub.gwdg.de/diss/2002/ahmed/ahmed.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Ishikura, Yukiko. "In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225510.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Jin, Yehwa. "Research towards the effective disruption of reproductive competence in Nile tilapia Oreochromis niloticus." Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28382.

Full text

Abstract:

Reproductive containment in farmed fish is highly desired for sustainable aquaculture to prevent genetic introgression with wild conspecifics and enhance productivity by suppressing sexual maturation. A number of strategies have already been implemented or have been tested in commercially important fish (e.g. triploidy, monosexing, hormonal therapies); however, they either do not result in 100% containment, or they cannot be applied to all species. One promising new approach consists in disrupting primordial germ cells (PGCs), at the origin of germline cells, to induce sterility. The work carr

APA, Harvard, Vancouver, ISO, and other styles

31

Mouka, Aurélie. "Analyse des variations du nombre de copies d'ADN dans une cohorte d'hommes infertiles et génération de modèles génétiques d’étude de la méiose à partir de cellules iPS de patients infertiles." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS300/document.

Full text

Abstract:

L’infertilité représente un problème majeur de santé publique en concernant 10 à 15% des couples en âge de procréer. Un facteur masculin est responsable de l’infertilité du couple dans près de la moitié des cas. Pour environ 30% d'entre eux, l'étiologie reste inexpliquée. Le premier axe du travail a concerné l’étude moléculaire d’une cohorte de patients infertiles (azoospermie non-obstructive/cryptozoospermie ou désordre du développement sexuel ou DSD) pour lesquels les analyses du caryotype standard et/ou des microdélétions des régions AZF par PCR n’ont pas permis d’expliquer le phénotype. L'

APA, Harvard, Vancouver, ISO, and other styles

32

Chen, Yi-Chen, and 陳奕臣. "Establishment of avian biodiversity preservation platform via primordial germ cells (PGCs)." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/5f4d35.

Full text

Abstract:

博士<br>國立臺灣大學<br>生物科技研究所<br>107<br>Primordial germ cells (PGCs) are the progenitors of gametes and could be seen as the key elements for germplasm preservation and the management of avian genetic biodiversity. To obtain the gametes and offspring, a recipient with no endogenous germ cells would be ideal to improve the germline transmission of the donor transplanted PGCs. In duck species, such bird exists as represented by the mule duck, produced by the intergeneric crossing between Muscovy drake (Cairina moschata) and female domestic duck (Anas platyrhynchos). This hybrid has normal somatic deve

APA, Harvard, Vancouver, ISO, and other styles

33

Ahmed, Manal Bayomi Mahmoud. "The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells." Doctoral thesis, 2002. http://hdl.handle.net/11858/00-1735-0000-0006-ABE4-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Hsu, Ya-Wen, and 許雅紋. "Targeted ablation of primordial germ cell (PGC) in the Larval zebrafish." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/03945949404705897651.

Full text

Abstract:

碩士<br>國立臺灣海洋大學<br>生物科技研究所<br>96<br>Escherichia coli Nitroreductase (NTR) is a enzyme that needs prodrugs to induce it. It can not only be used in non-divided or divided cells, but also controls the toxicity and time. When removing the prodrugs, the damaged cells will restore. Thus NTR is a very good tool to study the function and the development of organs. Use Sperm-t, SAMP14, ZP3, and AKAP would be specific in gonad to be the promoters and forming gene sequences. Use microinjection to settle the gene lines. Wait for approximately 3 months , cut part of fishtail to get genomic DNA .Test them t

APA, Harvard, Vancouver, ISO, and other styles

35

Li, Chun-Hsien Vincent, and 李俊賢. "Cryopreservation of primordial germ cells of poultry embryos." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/92299986893820737573.

Full text

Abstract:

碩士<br>國立成功大學<br>生物科技研究所<br>89<br>This thesis aims at preserving the PGCs (primordial germ cells) from domestic poultry species, including Native Chicken, White Leghorn, Muscovy, White Tsaiya and Brow Tsaiya. It will be a convenient and constant source of PGCs for the production of transgenic poultry. Meanwhile, it also provide an alternative way in preserving germplasm of poultry, especially the female genome, which can not be achieved by freezing of spermatozoa.The freezing solution composes of cultrue medium M199,chicken serum (CS) and dimethyl sulfoxide (DMSO). The percentage of DMSO are fi

APA, Harvard, Vancouver, ISO, and other styles

36

Wang, I.-Ling, and 王怡玲. "Non-viral Transfection of Duck Primordial Germ Cells." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/33002962487866247531.

Full text

Abstract:

碩士<br>國立成功大學<br>生物科技研究所<br>89<br>This project firstly aims at setting up the optimal transfection conditions for duck PGCs(primordial germ cells) by nonviral methods, including lipofection with FuGENE and electroporation. The efficiency of pEGFP-N1 transfection in duck PGCs by these methods will be compared. Then, the transfected PGCs will be microinjected into recipient duck embryos of stage 14-16. The gonad will be isolated when the manipulated embryos reached stage 27-28 or stage 32-34, and the exogenous DNA will be detected by PCR amplification. For the transfection conditio

APA, Harvard, Vancouver, ISO, and other styles

37

Lou, Chiao-Yun, and 樓喬云. "Isolation and culture of chicken primordial germ cells." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/92041653922027444139.

Full text

Abstract:

碩士<br>國立中興大學<br>動物科學系所<br>104<br>Primordial germ cells (PGCs）, the progenitor cells of gametes, are able to differentiate into spermatozoa and oocytes, which carry the genetic information throughout generation by sexual reproduction. Therefore, with the germ line-transmissible ability, PGCs hold the potential to produce transgenic animals and could be used as a versatile tool for the conservation of endangered animals. However, an optimized in vitro culture system to expand the number of PGCs and keep their undifferentiated status is required before application of PGCs-related techniques. Henc

APA, Harvard, Vancouver, ISO, and other styles

38

Cheng, Yu-Chi, and 鄭鈺錡. "To evaluate three labeling approaches of chicken primordial germ cells." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/94865676417997403389.

Full text

Abstract:

碩士<br>國立中興大學<br>生命科學系所<br>100<br>In early developmental stages of chick embryo, primordial germ cells (PGCs) derived from the germinal crescent and migrated through the blood circulation into the genital ridge, which later develop into gonad. After entering the gonad, PGCs differentiate into germ cells. The migration and differentiation mechanisms of PGCs vary among different species. The PGC migration path in zebrafish and mouse was reported by transgenic animal and live recording. However, in chicken embryos, only time snaps of PGC migration process were reported; yet a complete recording of

APA, Harvard, Vancouver, ISO, and other styles

39

Blaser, Heiko. "Molecular and cellular Mechanisms controlling Primordial Germ Cell Migration in Zebrafish." Doctoral thesis, 2006. http://hdl.handle.net/11858/00-1735-0000-0006-B6DD-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Huang, Alexander, and 黃建璋. "Production of germline chimeric ducks by transfer of primordial germ cells." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/90332166500720257376.

Full text

Abstract:

碩士<br>國立成功大學<br>生物科技研究所<br>88<br>The purpose of this experiment was to produce germline chimeric ducks by transferring the primordial germ cells（PGCs）from the different duck species.PGCs about 200 as the donor cells from the gonads of the advance incubated donor Muscovy duck embryos was transplanted into the blood circulation of the advance incubated embryos of the Brown Tsiya ducks as the injected recipient target by the microinjection technology, and was recovered to perform the migration movement continuously by nature in the blood vessels of these recipient embryos. The Brown Tsiya ducks（B

APA, Harvard, Vancouver, ISO, and other styles

41

Chang, Yung-Hsuan, and 張永琁. "In Vitro Reprogramming and Differentiation Potential of Rabbit Primordial Germ Cells." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/85241971762234982898.

Full text

Abstract:

碩士<br>東海大學<br>畜產與生物科技學系<br>103<br>Primordial germ cells (PGCs) starting as germline precursor cells can give rise to mature germ cells in vivo via a cascade of differentiation processes. They can also be reprogrammed in vitro into pluripotent stem cells, i.e., embryonic germ cells (EGCs). To understand the rabbit PGC migration process in early developmental stages, we anatomized New Zealand White rabbit fetuses at different days of development. Before 9.5 dpc, PGCs in the rabbit fetus were hardly isolatable due to the undistinguishable structure of genital ridge, whereas at the same time point

APA, Harvard, Vancouver, ISO, and other styles

42

Strasser, Markus. "Molecular Mechanisms in Primordial Germ Cell Development in Zebrafish." Doctoral thesis, 2007. http://hdl.handle.net/11858/00-1735-0000-0006-ACDA-C.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Gonzales, David S. "Gonadal germ cell chimeras produced by microinjecting primordial germ cells into the vasculature of early chicken embros [sic]." 1989. http://catalog.hathitrust.org/api/volumes/oclc/22301916.html.

Full text

Abstract:

Thesis (M.S.)--University of Wisconsin--Madison, 1989.<br>Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 59-65).

APA, Harvard, Vancouver, ISO, and other styles

44

Lin, Hsiu-Hung, and 林修弘. "Construction of HBsAg Plasmid and Transfection of Chicken Primordial Germ Cells and Spermatozoa." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/95571799464756909125.

Full text

Abstract:

碩士<br>國立成功大學<br>生物科技研究所碩博士班<br>91<br>Edible vaccines have been studied in transgenic plants, and it has been shown that transgenic plant containing human hepatitis B virus antigen (HBsAg) was able to induce a primary immune response. This study is going to establish the protocols for producing HBsAg by poultry egg. The HBsAg gene was construced into the vector of pEGFP-N1 and transfected into the cells of gonads of chicken embryos at stage 26-28 by electroporation. After three days of culture, the cells pellet which contained primordial germ cells (PGCs) and the cultured medium of transfecte

APA, Harvard, Vancouver, ISO, and other styles

45

Lu, Huang-Ting, and 盧奐婷. "Establishment of the long-term in vitro culture system for chicken primordial germ cells." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/u5n4rd.

Full text

Abstract:

碩士<br>國立成功大學<br>生物科技研究所碩博士班<br>92<br>Embryonic germ cells derived from primordial germ cells (PGCs) are undifferentiated and pluripotent. The objective of this study was to establish the long-term in vitro culture system for chicken PGCs. Primitive gonads were collected from 5.5-day-old (stage 28) chicken embryos and dissociated in 0.05% trypsin solution supplemented with 0.53mM EDTA. After centrifugation at 200×g for 5 min, these cells were seeded onto 4-well plates which were pre-coated with 0.1% gelatin. Each of the four different conditioned medium [CEF (non-inactivation), CEF (inactivatio

APA, Harvard, Vancouver, ISO, and other styles

46

Chiang, Chien-Yueh, and 江芊玥. "Production of mouse primordial germ cell-like cells by Xenopus egg extracts in conditioned medium." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/unkgn6.

Full text

Abstract:

博士<br>國立中興大學<br>動物科學系所<br>107<br>The female germ cells develop until the generation of mature oocytes that receive male gametes, which ensures the continuity of life. There are many challenges in in vitro reconstitution of the female germ cell lineage in reproductive research. Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be induced into primordial germ cell-like cells (PGCLCs) with the potential for regeneration of both genders of gametes. Even though the somatic or differentiated cells can be reprogrammed into iPSCs, they need to go through many stages of inducti

APA, Harvard, Vancouver, ISO, and other styles

47

Bernardo, Ana Isabel de Melo 1988. "Hitchhiking through the amnion/chorion: redefining the migratory route of primordial germ cells in chicken embryos." Master's thesis, 2011. http://hdl.handle.net/10451/4531.

Full text

Abstract:

Tese de mestrado. Biologia (Biologia Evolutiva e do Desenvolvimento). Universidade de Lisboa, Faculdade de Ciências, 2011<br>Primordial germ cells (PGCs) are the founders of the gametes. They are one of the first discernible embryonic cell types which are set aside very early in the developing embryo. As it happens in other organisms, in chicken embryos, PGCs migrate from their extraembryonic location to combine with the somatic component of the gonad. An ingression of PGCs from the hypoblast into the vascular system, around stage HH8-10, was previously addressed using SSEA-1 as a marker for P

APA, Harvard, Vancouver, ISO, and other styles

48

Mendes, Sara Mariana Eugénio Ferraz. "Testing a mesendoderm protocol in Smad5 and Smad 1/5 depleted embryonic stem cells for primordial germ cell induction." Master's thesis, 2015. http://hdl.handle.net/10451/22653.

Full text

Abstract:

Tese de mestrado em Biologia Evolutiva e do Desenvolvimento, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2015<br>As Células Germinais Primordiais (CGPs) são responsáveis pela continuidade das espécies. A formação de CGPs é dependente de uma rede complexa de sinalização molecular coordenada no espaço e no tempo durante o desenvolvimento embrionário. A especificação destas células consiste em manter as células pluripotentes durante o desenvolvimento do animal, até que este atinja a maturidade e seja capaz de se reproduzir. A instrução destas células vai no sentido opo

APA, Harvard, Vancouver, ISO, and other styles

49

Wei, Ke-Hsuan, and 魏可軒. "Primordial germ cells require heparan sulfate to properly migrate and multiply during early embryonic development in the zebrafish Daino rerio." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/37754369467624708039.

Full text

Abstract:

碩士<br>國立臺灣大學<br>動物科學技術學研究所<br>101<br>Heparan sulfate glycosaminoglycan (HS-GAG) is a linear polysaccharide ubiquitously residing on the animal cell surface and the extracellular matrix usually in the form of heparan sulfate proteoglycans. It binds many proteins, such as various chemokines, growth factors and morphogens, and in turn modulates intracellular signal transduction. After specified, zebrafish primordial germ cells (PGCs) migrate following specific routes from their sites of origin to gonadal ridge during early embryonic development. Previous studies showed that HS-GAGs participate

APA, Harvard, Vancouver, ISO, and other styles

50

Wolff, Eva. "Embryonic and foetal germ cell development in the marmoset monkey: comparative in situ and cell culture studies." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E4FB-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!