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Simandjuntak, Suddin, and Mokosuli Samuel. "Isolation and Identification of Thermophilic Bacteria, Producer of Amylase Enzyme, from Lake Linow, North Sulawesi." Journal of Pure and Applied Microbiology 12, no. 2 (June 30, 2018): 543–54. http://dx.doi.org/10.22207/jpam.12.2.13.
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Lalam, Chandra Mouli, P. Y. Naidu, and T. Srinivasan. "Isolation and Screening of Lactobacillus Bacteria for Ability to Produce Antibiotics." International Letters of Natural Sciences 32 (January 2015): 68–76. http://dx.doi.org/10.18052/www.scipress.com/ilns.32.68.
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Antibiotic is one of the important commercially exploited secondary metabolites produced by bacteria and used in a wide range. Most of the antibiotics used today are isolated from the microbes. Bacteria are easy to culture, isolate, maintain and to improve their strain. Isolation of lactic acid bacteria (LAB) from soil, yoghurt and cheese was carried out. LAB were cultivated on De Man Rogosa Sharpe (MRS) agar and were characterized based on colony morphology, cell shape and biochemical tests. Out of eight samples analyzed, 96 isolates were identified as LAB. The bacterial isolates were identified as Lactococcus Lactis, Lactobacillus Brevis, Lactobacillus casei, Pediococcus damnosus, Lactobaciilus rhamnosus, Lactobacillus Plantarum, Lactobacillus pentosus, Enterococcus feacalis, Staphylococcus Simulans.Using MRS broth, the isolated LAB were screened for production of bacteriocins. Further, 96 LAB screened for bacteriocin production, 12 LAB were identified as bacteriocin producers. Out of 12 LAB, Enterococcus faecium (CST-1) was identified as potential bacteriocin producer against Bacillus subtlis MTCC-10403 Pseudomonas aureginosa MTCC-4676 microorganisms used while Pediococcus damnosus had the least bacteriocin activity against Staphylococcus aureus and Bacillus subtilis.
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Shah, Binod Kumar, Sapana Sharma, Gita Shakya, and Bishnu Prasad Upadhyay. "Multiple drug resistant Vibrio cholerae, Salmonella and Shigella from Nepalgunj Cholera outbreak and different hospitals of Nepal." Nepalese Journal of Biosciences 2 (January 24, 2013): 31–39. http://dx.doi.org/10.3126/njbs.v2i0.7487.
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Diarrhoea is a major health problem throughout the world, and responsible for high morbidity and mortality in Nepal. The crosssectional prospective study was carried out to screen ESBL producer from MDR Vibrio Cholerae, Salmonella and Shigella from 268 diarrhoeal stools from Nepalgunj Cholera outbreak and different hospitals of Nepal during April 2010 to January 2011. The specimens were processed by standard microbiological methods and confirmed with serology. Altogether 14.18% of bacteria were isolated with 8.21% V. cholerae O1 El Tor Ogawa, 2.24% Shigella flexneri B and 3.73% Salmonella spp. Highest bacterial culture (47.36%) were isolated in Kathmandu while highest V. cholerae (77.27%) were isolated in Nepalgunj. The highest number of Salmonella spp. and Shigella spp. were isolated from Kanti Children’s Hospital. Highest bacteria isolation (47.36%) and highest V. cholerae isolation (81.81%) were observed in the August. The bacteria isolation was significantly associated with places and months (p<0.05). However, there was no statistical difference in the bacteria isolation with sex (P>0.05). 100% V. cholerae, 100% Shigella spp. and 80% Salmonella spp. were MDR while only one Salmonella Cholerasuis was found ESBL producer. DOI: http://dx.doi.org/10.3126/njbs.v2i0.7487 Nepalese Journal of Biosciences 2 : 31-39 (2012)
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Leonita, Shinta, Maria Bintang, and Fachriyan Hasmi Pasaribu. "Isolation and Identification of Endophytic Bacteria from Ficus variegata Blume as Antibacterial Compounds Producer." Current Biochemistry 2, no. 3 (November 23, 2016): 116–28. http://dx.doi.org/10.29244/cb.2.3.116-128.
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Endophytic bacteria can produce antibacterial compounds. Their existence in the medicinal plant of Ficus variegata Blume enables the production of bioactive compounds similar to those contained by the host plants. The purpose of this study was to isolate and identify endophytic bacteria from F. variegata which is potential to produce antibacterial compounds. The methods used include isolation of endophytic bacteria from leaves, stem, aerial root, and fruit of F. variegata plants. Antibacterial activity assay was done against four types of bacteria i.e. Staphylococcus aureus, Bacillus subtillis, Escherichia coli, and Psedomonas aeruginosa. Identification of endophytic bacteria was conducted based on morphological analysis, biochemical test, and molecular analysis of 16S rRNA. Endophytic bacterial culture was extracted by ethyl acetate and analyzed by GC-MS. A total of 29 isolates of endophytic bacteria were obtained from F. variegata. The BH2 isolate was found to have potential activity. Analysis of 16S rRNA showed that BH2 isolate was related to Pseudomonas aeruginosa strain SV1 with 99 % identity. The result of GC-MS analysis showed that the antibacterial compound was Nonanoic acid ethyl ester
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Abdul Rahim, Ainihayati, Wan Nurul Hakimah Wan Azmi, Noor Azlina Ibrahim, Wan Nurul Iffan Sofea Wan Mohamad Safari, and Khomaizon Abdul Kadir Pahirul Zaman. "Isolation of Potential Biosurfactant Producer from Oil Contaminated Soil and Water." Journal of Tropical Resources and Sustainable Science (JTRSS) 5, no. 3 (December 31, 2017): 140–44. http://dx.doi.org/10.47253/jtrss.v5i3.659.
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Biosurfactants are extracellular macromolecules produced by bacteria, yeast, and fungi when grown on different carbon sources. It has the ability to reduce the surface tension of a liquid, interfacial tensions between two liquids and between a liquid and a solid. This study was conducted to isolate potential biosurfactant producers from oil-contaminated soil and water. Soil and water samples were obtained from the food court area in front of Universiti Malaysia Kelantan, Jeli Campus. Isolation of biosurfactant producing bacteria was carried out on minimal salt medium (MSM) supplemented with palm oil as the sole carbon source. Five potential biosurfactant producers; WS2, WS4, WS5, SS2 and SS5 were successfully isolated and identified by 16S rRNA analysis. Isolate WS4, SS2 and SS5 showed the highest similarity to Klebsiella sp and the other two isolates, WS2 and SS5 showed the highest similarity to Pseudomonas sp. and Nanobacterium sp. respectively. While Klebsiella sp. and Pseudomonas sp. were reported as prevalent biosurfactant producer, no report is available on the production of biosurfactants by Nanobacterium sp. All isolates showed variation in biosurfactant characterization assays which are emulsification test, drop collapse test, oil spreading test, blood haemolysis and blue agar plate assay.
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Tilay, Ashwini, and Uday Annapure. "Novel Simplified and Rapid Method for Screening and Isolation of Polyunsaturated Fatty Acids Producing Marine Bacteria." Biotechnology Research International 2012 (August 15, 2012): 1–8. http://dx.doi.org/10.1155/2012/542721.
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Bacterial production of polyunsaturated fatty acids (PUFAs) is a potential biotechnological approach for production of valuable nutraceuticals. Reliable method for screening of number of strains within short period of time is great need. Here, we report a novel simplified method for screening and isolation of PUFA-producing bacteria by direct visualization using the H2O2-plate assay. The oxidative stability of PUFAs in growing bacteria towards added H2O2 is a distinguishing characteristic between the PUFAs producers (no zone of inhibition) and non-PUFAs producers (zone of inhibition) by direct visualization. The confirmation of assay results was performed by injecting fatty acid methyl esters (FAMEs) produced by selected marine bacteria to Gas Chromatography-Mass Spectrometry (GCMS). To date, this assay is the most effective, inexpensive, and specific method for bacteria producing PUFAs and shows drastically reduction in the number of samples thus saves the time, effort, and cost of screening and isolating strains of bacterial PUFAs producers.
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Kaur, Kuldeep, Vikrant Dattajirao, Vikas Shrivastava, and Uma Bhardwaj. "Isolation and Characterization of Chitosan-Producing Bacteria from Beaches of Chennai, India." Enzyme Research 2012 (August 5, 2012): 1–6. http://dx.doi.org/10.1155/2012/421683.
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Chitosan is a deacetylated product of chitin produced by chitin deacetylase, an enzyme that hydrolyses acetamido groups of N-acetylglucosamine in chitin. Chitosan is a natural polymer that has great potential in biotechnology and in the biomedical and pharmaceutical industries. Commercially, it is produced from chitin via a harsh thermochemical process that shares most of the disadvantages of a multistep chemical procedure. It is environmentally unsafe and not easily controlled, leading to a broad and heterogeneous range of products. An alternative or complementary procedure exploiting the enzymatic deacetylation of chitin could potentially be employed, especially when a controlled and well-defined process is required. In this study, 20 strains of bacteria were isolated from soil samples collected from different beaches of Chennai, India. Of these 20 bacterial strains, only 2 strains (S3, S14) are potent degrader of chitin and they are also a good producer of the enzyme chitin deacetylase so as to release chitosan.
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SUNNY, FRANSISCA, TRI HANDAYANI KURNIATI, and ARIANI HATMANTI. "ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR." BIOMA 11, no. 1 (March 30, 2015): 42. http://dx.doi.org/10.21009/bioma11(1).5.
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ABSTRACT
Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial compounds. coral and sponge-ssociated bacteria are potential producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with Staphylococcus aureus, Bacillus subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate was isolated from corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid.
Key words: antibacterial activity, association, characterization, coral, isolation, sponge
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A N Zamani, N., T. E Tengku Zainal Mulok, R. Mat Nor, and . "Isolation and Molecular Identification of Biosurfactant Producing Soil Bacteria." International Journal of Engineering & Technology 7, no. 4.14 (December 24, 2019): 77. http://dx.doi.org/10.14419/ijet.v7i4.14.27475.
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Biosurfactants are amphiphilic compound, having hydrophilic and hydrophobic moieties enabling them to reduce surface and interfacial tension at the surface. Their unique properties are applied in various industries such as foaming and wetting agents, emulsifiers, detergents and bioremediation. A total of 98 isolates showed biosurfactant activity using hemolytic activity, drop collapse test and oil spreading assay. All isolates were rod-shaped, Gram positive and majority of them were non-endospore former. Only the isolates showing the highest percentage of emulsification index (E24) and ability to reduce tension were used for species identification using 16S rDNA gene sequencing which were isolates A1(6) and A2(1). Both isolates were identified as Bacillus sp. cp-h50 and Bacillus sp. XT-24 respectively, rod-shaped, endospore former and Gram positive. The biosurfactant produced by both species showed high emulsification index (E24) (A1(6), 63.3% and A2(1), 46.7%) and good surfactant capacity. The size of amplified gene of 16S rDNA gene was approximately 1.5 kb. These features provide evidence that both species could be a potential biosurfactant producer with proper optimization for the production of biosurfactant. The biosurfactant produced by both bacterial species were identified as surfactin using Fourier Transform Infrared Spectroscopy (FTIR).
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İspirli, Hümeyra, and Enes Dertli. "Isolation and identification of exopolysaccharide producer lactic acid bacteria from Turkish yogurt." Journal of Food Processing and Preservation 42, no. 1 (May 24, 2017): e13351. http://dx.doi.org/10.1111/jfpp.13351.
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KHALILA, RUHUL, Lenni Fitri, and SUHARTONO SUHARTONO. "Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia." Microbiology Indonesia 14, no. 1 (August 11, 2020): 4. http://dx.doi.org/10.5454/mi.14.1.4.
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Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.
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Mulyawati, Alifia Issabella, Tri Ardyati, and Yoga Dwi Jatmiko. "Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa." Jurnal Biodjati 4, no. 1 (May 28, 2019): 21–30. http://dx.doi.org/10.15575/biodjati.v4i1.4194.
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Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations.
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Saputri, Dina Dyah, Maria Bintang, and Fachriyan H. Pasaribu. "Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer." Current Biochemistry 2, no. 2 (August 3, 2015): 86–98. http://dx.doi.org/10.29244/cb.2.2.86-98.
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Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.
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Aslam, Muhammad, Faiz-Ul-Hassan Nasim, Rana Ruhi, Hassan Murad, Samina Ejaz, Muhammad Shafiq Choudhary, Ghulam Mustafa, Muhammad Ashraf, and Jameel Rehman. "Isolation and Characteristics of Biotechnologically Important Antagonistic Thermophilic Bacteria from Rhizosphere of Haloxylon salicornicum." Polish Journal of Microbiology 67, no. 1 (March 9, 2018): 49–58. http://dx.doi.org/10.5604/01.3001.0011.6142.
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Rhizobacteria are an active part of microbial population in the rhizosphere of plants. In this study, twenty rhizobacteria were isolated from the rhizosphere of a perennial grass, Haloxylon salicornicum, found in Cholistan desert, an arid landmass near Bahawalpur Pakistan, in one set of experimental conditions. Colony characteristics, biochemical and molecular analyses of these isolates were performed. All isolates were bacilli, gram positive with off-white colonies and exhibited typical bacilli colony morphology. None of the isolates was gelatinase, urease, indole, H2S and catalase producer. Eleven isolates were amylase producers and 8 isolates were acid producers. All isolates fermented glucose, 3 fermented lactose and 19 fermented fructose. Molecular data revealed that out of twenty isolates, 14 isolates showed 91–99% identity with Brevibacillus borstelensis, 4 with Bacillus subtilis (97–98%) and 2 with Bacillus licheniformis (94–99%) through BLAST analysis. All identified bacterial isolates cladded with their respective groups in the phylogenetic tree. Many (11–15 out of 20) of the isolates were more effective in inhibiting growth of the tested bacterial strains as compared to the positive control (Ampicillin 50 μg/disc). We conclude that bacilli are the predominant form populating rhizosphere of this desert grass. Among the isolated bacteria Brevibacillus borstelensis, Bacillus subtilis and Bacillus licheniformis are the most predominant species.
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Ertanto, Tomi. "Isolation of Poly-3-Hydroxyalkanoates (PHA) producer bacteria as eco-friendly biodegradable plastic." Journal of Bioscience and Bioengineering 108 (November 2009): S81. http://dx.doi.org/10.1016/j.jbiosc.2009.08.238.
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Khan, Ruby. "ISOLATION OF ANTIBIOTIC PRODUCING BACTERIA FROM SOIL." Current Trends in Natural Sciences 10, no. 19 (July 31, 2021): 407–15. http://dx.doi.org/10.47068/ctns.2021.v10i19.054.
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Antibiotics are a major secondary metabolite produced by a wide range of bacteria. The microbes developed various antibiotics that could be used to treat various infectious diseases. Are useful In vitro isolation, the culture and care of bacteria are quite simple, and we can easily improve their stress. The main soil pathogens of the Bacillus species are caused by important antibiotics such as bactericidal Endospores produced by the Bacillus species are very resistant. They are always found to inhibit the growth of other microbes. In the present research study, soil bacteria with antimicrobial activity have been screened and isolated. Subsequently, various pathogenic bacterial lawns were prepared to check the antimicrobial activity against various pathogens. Different zones are observed against different pathogenic bacteria. Comparison of antimicrobial activity of soil isolation with different antibiotic discs as well as various pathogenic bacteria. A clear zone of soil isolates of 5 mm, 15 mm, 21 mm, 12 mm, 30 mm, 32 mm and 40 mm against germs or pathogenic bacteria. The zones produced by antibiotic discs against pathogenic bacteria were zones of 5 mm, 10 mm, 12 mm, 15 mm, 20 mm, and 21 mm observed.
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Aktar, Lovely, Farhana Islam Khan, Tahmina Islam, Shawon Mitra, and Mihir Lal Saha. "Isolation and Characterization of Indigenous Lipase Producing Bacteria from Lipid?rich Environment." Plant Tissue Culture and Biotechnology 26, no. 2 (December 10, 2016): 243–53. http://dx.doi.org/10.3329/ptcb.v26i2.30574.
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To isolate and characterize lipase producing bacteria from lipid?rich environment and screen the best lipolytic indigenous bacteria a study was made. For the isolation of bacteria, oil based wastewater and soil were collected from ten different sampling sites. Four different media were used for study the aerobic heterotrophic bacterial count. The highest bacterial count (1.56 × 107 cfu/gm) was observed in dairy farm soil and lowest (8.3 × 102 cfu/ml) in the Buriganga river water. The highest percentage (94.51) of lipase producing bacteria was found in edible oil mill soil and lowest (23.44) in the Buriganga river water. Among the total isolates 30 showed better lipase activity. Potential ten lipase producers were taken for molecular identification. Among them, nine genera were matched with their conventional identification but conventionally identified Acetobacter liquifaciens was found to be as Stenotrophomonas maltophilia. The enzyme produced by the isolated bacteria could be used for the treatment of lipid?rich wastewater.Plant Tissue Cult. & Biotech. 26(2): 243-253, 2016 (December)
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Jannah, Akyunul, Aulanni`am Aulanni`am, Tri Ardyati, and Suharjono Suharjono. "Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran." Indonesian Journal of Chemistry 18, no. 3 (August 30, 2018): 514. http://dx.doi.org/10.22146/ijc.26783.
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Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 16S rDNA showed that BE 8 belongs to Bacillus subtilis and BE 14 in Bacillus cereus.
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Islam, MS, M. Abdullah Yusuf, M. Shah Jahirul Hoque Chowdhury, and M. Afzal Hossain. "ESBL producing Gram Negative Aerobic Bacteria Isolated from Burn Wound Infection with Their Antibiogram in Dhaka." Journal of Science Foundation 10, no. 2 (February 14, 2014): 63–69. http://dx.doi.org/10.3329/jsf.v10i2.17959.
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Background: Infection is an important cause of mortality in burns. Rapidly emerging nosocomial pathogens and the problem of multi-drug resistance necessitates periodic review of isolation patterns and antibiogram in the burn ward. Objective: The purpose of the present study was to see the frequency of bacteria in burn wound with their antibiotic sensitivity pattern. Method: This cross sectional study was conducted in the laboratory of Department of Microbiology at Dhaka Medical College, Dhaka and samples were collected from the burn unit of Dhaka Medical College Hospital, Dhaka. Bacterial isolates from 108 wound swabs taken from burn patients were identified by conventional biochemical methods and antimicrobial susceptibility was performed. Result: Out of 98 bacteria E. coli (20.4%) was most common and 25.0% of these bacteria were ESBL producer. Out of 14 Klebsiella species ESBL producer was in 6(42.9%). ESBL producing Proteus species (21.4%) and Pseudomonas species (14.9%) were also detected. Klebsiella (33.91%) was the predominant organism closely followed by Pseudomonas (31.84%). Conclusion: E. coli is the most common ESBL producing bacteria causing the burn wound infection. DOI: http://dx.doi.org/10.3329/jsf.v10i2.17959 J Sci Found, 2012;10(2):63-69
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Cacchio, Paola, and Maddalena Del Gallo. "A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications." Geosciences 9, no. 11 (November 14, 2019): 479. http://dx.doi.org/10.3390/geosciences9110479.
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Bacterial calcium-carbonate precipitation (BCP) has been studied for multiple applications such as remediation, consolidation, and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper, we studied CaCO3 precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria, and Alphaproteobacteria are the dominant bacteria involved in CaCO3 precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO3, mostly in the form of calcite. In this study, Biolog® EcoPlate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO3 producer strains.
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Mubarik, Nisa Rachmania, and Iah Novi Maslahah. "Isolation and Characterization of Indole-3-Acetic Acid Producing Bacteria from Cow Urine." Jurnal Biodjati 4, no. 1 (May 28, 2019): 96–104. http://dx.doi.org/10.15575/biodjati.v4i1.4481.
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Cow urine contains urea as nitrogen source, therefore it can be expected to isolate the beneficial bacteria for plants, for example indole-3-acetic acid (IAA) or auxin producing bacteria. The objective of research was to obtain IAA producing bacteria from cow urine, to characterize bacterial isolate, and to measure its ability to stimulate the growth of green bean seedlings (Vigna radiata). The methods used in this study were collecting urine from cow cattle, obtaining IAA-pro-ducing bacteria from urine, measuring IAA using Salkowski method, and applying selected bacterial supernatants on green bean seedling plants. The number of IAA producing bacteria that was successfully purified was 18 isolates. There are five isolates, namely US 5, BS1, BS 2, BS 4 and BS 5 which have the ability to solubelize phosphate on Pikovskaya agar. The five isolates were also able to fix free nitro-gen on N Free media and did not show hypersensitivity on tobacco leaves. The results of the growth of isolates in blood agar showed positive for US 5 and BS 2 as beta hemolysin producers. Further-more, isolate BS 4 was chosen to produce exogenous IAA quantita-tively. Isolate BS 4 produced IAA 6.364 ppm at the 45 h incubation at stationary phase. The use of BS 4 supernatant on green bean seed-lings showed an effect on plant height and lateral root length better than control (without treatment) on 6 days after planting. Morpho-logical characteristic of isolate BS 4 was rod shape, Gram positive, endospore producing, aerobic, and had similarity with genus Bacillus
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Prihatiningsih, Nur, Heru Adi Djatmiko, and Puji Lestari. "SCREENING OF COMPETENT RICE ROOT ENDOPHYTIC BACTERIA TO PROMOTE RICE GROWTH AND BACTERIAL LEAF BLIGHT DISEASE CONTROL." Jurnal Hama dan Penyakit Tumbuhan Tropika 20, no. 1 (April 26, 2020): 78–84. http://dx.doi.org/10.23960/j.hptt.12078-84.
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Screening of competent rice root endophytic bacteria to promote rice growth and bacterial leaf blight disease control. This study was aimed to collect isolate endophytic bacterial of rice roots which able to produce IAA, determine the effect of endophytic bacteria application in stimulating rice plant growth, and evaluate the potential of rice root endophytic bacteria for controlling bacterial leaf blight. This reasearch was carried out at the Screen House, Plant Protection Laboratory, and Agrohorti Laboratory of the Agriculture Faculty, Jenderal Soedirman University. Isolation of rice root endophytic bacteria was carried out by purposive random sampling from several marginal lands. The results showed that 8 isolates of rice root endophytic bacteria were able to produce IAA, ranging from 57.56 to 79.33 ppm and B07 isolate from Serayu produced the highest amount of IAA. The B04 and B07 isolates were contributed to increase the rice plant growth. The application of rice root endophytic bacteria was effective in controlling bacterial leaf blight.
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Belfiore, Carolina, Ana P. Santos, Manuel Contreras, and Maria E. Farias. "Isolation and Characterization of Plant Growth Promoting Bacteria Isolated from Andean Soil as Potential Inoculants of Soybean Seeds." Environment and Natural Resources Research 8, no. 3 (September 30, 2018): 203. http://dx.doi.org/10.5539/enrr.v8n3p203.
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Argentina is the leading exporter of soybean oil and flour, and the third largest producer of grain. Since, the crop is a matter of great importance to the national economy. Their production depends on the soil as their main resource to ensure a good productive capacity, so it is necessary to preserve the physical, chemical and biological properties of the soil. Although, the indiscriminate use of chemical fertilizers, disturb them. In recent years, there has been a trend towards cleaner production to reduce the use of chemical. One of the alternatives involves biological means through the use of plant growth promoting bacteria. These group of bacteria colonize the rhizosphere of plants and stimulate the plant growth by several mechanisms.
The objective of this work was to characterize, identify and evaluate the growth promoting effect of 13 strains isolated from the Andean vegetation rhizosphere. The bacterial isolates were Enterobacteria, Stenotrophomonas, Pseudomonas, Nocardiodes, Bacillus, Exiguobacterium, Acinetobacter and Lactococcus genera. The results of the biochemical characterization determined that from the 13 bacterial strains, which produce siderophores, 11 possess the catalase enzyme, 10 fixate nitrogen, 12 produce the protease enzyme, 12 solubilize phosphorus, and 11 produce indoleacetic acid.
The application of different inoculums to the seeds, allowed to obtain plants with longer stem length, more developed roots, larger and more intense coloration leaves than the control plants. The results encourage deeper studies to achieve the formulation of inoculums to use as a biofertilizer, which would replace chemical fertilizers or reduce their doses.
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Rangaswamy, B. E., K. P. Vanitha, and Basavaraj S. Hungund. "Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit." International Journal of Polymer Science 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/280784.
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Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified asGluconacetobactersp. RV28,Enterobactersp. RV11, andPseudomonassp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources usingGluconacetobactersp. RV28. The strain produced 4.7 g/L of cellulose at optimum growth conditions of temperature (30°C), pH (6.0), sucrose (2%), peptone (0.5%), and inoculum density (5%). Characterization of microbial cellulose was done by scanning electron microscopy (SEM).
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Liu, Xiao Ye, Zi Rui Guo, Yong Juan Zhang, Zhan Qing Wang, and Yong Feng Li. "The Molecular Characterization and Hydrogen Production of a New Species of Anaerobe in Wastewater Treatment Process for H2 Recovery." Advanced Materials Research 113-116 (June 2010): 1049–52. http://dx.doi.org/10.4028/www.scientific.net/amr.113-116.1049.
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The isolation and identification of hydrogen production bacteria(HPB)with high yield and high evolution rate is an important foundation of fermented hydrogen production process by anaerobic digesting high strength organic wastewater. An improved Hungater rolling tubes technique and a plate method of culture bottle (PMCB) were employed to enumerate and isolate the anaerobes. The HPB-RL medium was designed specially for isolating and culturing anaerobic hydrogen bacteria. Culture temperature was at 37°C and 5.5 of pH. About 90 strains of bacteria were isolated, in which, a strain of the hydrogen producer with high yield and high evolution rate was isolated and named Rennanqi12. The isolates were straight rods, Grams positive, strictly anaerobic, no spore-forming bacteria, whole bacteria flagella, having capsule, 3~4 metachromatic granules. The strain of Rennanqi12 could produce hydrogen using molasses wastewater as the carbon resource. The isolate Rennanqi12 has the closest relationship with the strain C. cellulose for the 94% homology of 16S rDNA. The strain of Rennanqi12 might be a new species that belongs to a new genus. The amount of hydrogen-producing (YH2)is 1880.5ml/L medium and the maximum hydrogen-producing rate(QH2)is 28.2 mmol/g•(dry•cell)•h when R12 digested substrates were glucose.
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Abdul Halim, Nabila Huda, Nur Syarafina Mohd Zahir, Nor Munirah Mohd Amin, and Hanani Ahmad Yusof @ Hanafi. "Distribution of Hyaluronidase-producing Staphylococcus aureus and Staphylococcus epidermidis Isolated from Palm Skin and Anterior Nares of Healthy Malaysian Adults." Journal of Clinical and Health Sciences 5, no. 1 (June 23, 2020): 42. http://dx.doi.org/10.24191/jchs.v5i1.9820.
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Introduction: The distribution of Staphylococcus aureus and Staphylococcus epidermidis among Malaysian healthy adults and their capability to produce enzyme hyaluronidase are less reported. Hyaluronidase degrade hyaluronic acid in animal connective tissue and facilitate bacterial spreading in host body. This study aims to identify the distribution of both Staphylococci species in healthy subject, the hyaluronidase producer among the isolates and the association of the latter with site of isolation (palm skin and anterior nares) and gender ofthe host. Methods: A total of 108 swab samples were collected from anterior nares and palm of 54 healthy subjects. The bacteria were identified through microscopic and biochemical tests, before screened for hyaluronidase production using hyaluronic acid diffusion rapid plate method. Results: Total of 139 bacterial isolates were identified; 68 isolates are S. aureus, 63 S. epidermidis and 8 other bacterial species. Staphylococcus aureus was highly isolated from palm (57%) than anterior nares (47%). On the contrary for S. epidermidis was highly isolated from anterior nares (53%) than from palm skin (43%). Equal proportion was found for both species in male and female subject. A total of 77 (59%) isolates produced hyaluronidase; 55%are S. aureus and 45% are S. epidermidis. Hyaluronidase-producer isolates are equally found between anterior nares (56%) and palm skin (61%) or male (57%) and female subject (60%) regardless of Staphylococcal species. No significant value was recorded for any analysis. Conclusion: Capability of commensal S. aureus and S. epidermidis isolated from healthy subject to produce hyaluronidase may indicate their potential as opportunistic pathogen whenever the opportunity arises in any way.
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Mahulette, Ferymon, Nisa Rachmania Mubarik, Antonius Suwanto, and Widanarni Widanarni. "Isolation and Characterization of Lactic Acid Bacteria from Inasua." Journal of Tropical Biodiversity and Biotechnology 1, no. 2 (April 11, 2017): 71. http://dx.doi.org/10.22146/jtbb.16380.
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Inasua is a traditionally product of wet salt fish fermentation produced by Teon, Nila and Serua (TNS) Communities in Central Maluku, Indonesia. The community made this fermented fish to anticipate the lean time when fisherman could not go to sea. The fish that used as inasua raw material is demersal fishes that live around coral reefs, such as Samandar fish (Siganatus guttatus), Gala-gala fish (Lutjanus sp.) and Sikuda fish (Lethrinus ornatus). The objective of the research was to isolate and characterize of bacterial indigenous in Inasua from three producers in Seram Island. The measurement of pH from inasua samples were 5.9, 5.0 and 5.8, respectively. The highest number of lactic acid bacteria was found from Gala – gala inasua was 2,5x107 cfu/g sample. Isolation of all isolates bacteria from inasua showed that a total of 7 isolates of bacteria was obtained from Samadar inasua, 9 isolates from Gala-gala inasua, and 7 isolates from Sikuda inasua. From a total of 23 isolates, only 6 isolates had characteristic as lactic acid bacteria that were Gram positive, negative catalase, and cocci shape. The microscopic characteristics of the isolates are coccid in pairs or uniforms which combine to form tetrads. Carbohydrate utilization test of selected isolate by using API 50 CHB kit indicated that 13 carbohydrates are fermented by these isolates after incubation for 48 hours. The research was concluded that the dominant bacteria in inasua sample is cocci-lactic acid bacteria.Keywords : fermented fish, inasua, lactic acid bacteria, MRSA medium
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Khalifeh, A., B. Roozbehani, A. M. Moradi, S. Imani Moqadam, and M. Mirdrikvand. "Isolation of Crude Oil from Polluted Waters Using Biosurfactants Pseudomonas Bacteria: Assessment of Bacteria Concentration Effects." Engineering, Technology & Applied Science Research 3, no. 2 (April 7, 2013): 396–401. http://dx.doi.org/10.48084/etasr.265.
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Biological decomposition techniques and isolation of environmental pollutions using biosurfactants bacteria are effective methods of environmental protection. Surfactants are amphiphilic compounds that are produced by local microorganisms and are able to reduce the surface and the stresses between surfaces. As a result, they will increase solubility, biological activity, and environmental decomposition of organic compounds. This study analyzes the effects of biosurfactants on crude oil recovery and its isolation using pseudomonas sea bacteria species. Preparation of biosurfactants was done in glass flasks and laboratory conditions. Experiments were carried out to obtain the best concentration of biosurfactants for isolating oil from water and destroying oil-in-water or water-in-oil emulsions in two pH ranges and four saline solutions of different concentrations. The most effective results were gained when a concentration of 0.1% biosurfactants was applied.
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Lee, Ng Sieh, and Ainihayati Abdul Rahim. "Isolation and Characterization of Polyhydroxyalkanoates (PHAs) Producers from Kg Batu Melintang hotspring." Journal of Tropical Resources and Sustainable Science (JTRSS) 8, no. 1 (July 13, 2021): 1–4. http://dx.doi.org/10.47253/jtrss.v8i1.482.
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The increasing awareness on the negative environmental impact of petroleum-based plastics has driven industries to explore more efficient biodegradable polymers for production of bioplastic. Polyhydroxyalkanoates (PHAs) is one of the potential biodegradable polymers to replace petroleum-based plastic. It is synthesized and accumulated as intracellular granules in microorganism. In this study, polyhydroxyalkanoates (PHAs) producing bacteria were successfully isolated from sediment collected from Kg. Batu Melintang hotspring. Isolation process was carried on Minimal Salt Medium (MSM) agar supplemented with excess glucose as a carbon source. Potential PHA producers were screened by using Nile Blue staining plate assay. Out of 144 bacterial isolates, 12 bacterial isolates which showed strong orange fluorescence under ultraviolet (UV) light (365nm) were selected for further identification by morphological characterization and biochemical analysis. Based on the result obtained, possible species for Gram positive rod shape bacteria B75 and B87 is Corynebacterium kutsceri meanwhile Gram negative rod shape bacteria A4, A12, A50, A68, B2, B13, B22, B31, B73 and C3 showed affiliation to Citrobacter sp., Enterobacter sp., Erwinia sp., Klebsiella sp., Proteus sp., Salmonella sp., Serratia sp., Shigella sp., and Yersinia sp.
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P., Yendania Grevitara, Badriyatur Rahma F., Hellen Septirangga P., Irma Dahlia Y., and Endang Suarsini. "Isolation and Identification of Cellulose Degrading Bacteria from Banana Peel Compost." El-Hayah 7, no. 1 (May 3, 2019): 6–11. http://dx.doi.org/10.18860/elha.v7i1.7241.
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Cellulolytic bacteria are bacteria that have the ability to hydrolyze cellulose complexes into smaller oligosaccharides and eventually become glucose. Glucose is used as a carbon and energy source for bacterial growth. This study was conducted to isolate the cellulose degrading bacteria from banana peel compost that produce cellulose enzymes based on the clear zone that visible around the colony. The cellulolytic activity was determined by the ability of bacteria to hydrolyze the Carboxymethyl Cellulose (CMC) substrate. Determination of cellulolytic activity is known based on cellulolytic index calculation, the diameter total minus the diameter of the colony and divided by the diameter of the colony. The result of five bacterial isolates was found but only one bacterium had the potential to be a cellulose degradation. Based on the Microbact Gram-Negative Identification System, the bacterium is Burkholderia cepacia. These bacteria have an important role in nature as decomposers of various complex compounds, such as cellulose, hemicellulose, lignin, and pectin.
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Mawati, Sefi Desfeni, Esti Harpen, and Hilma Putri Fidyandini. "SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN." Journal of Aquatropica Asia 6, no. 1 (July 6, 2021): 1–7. http://dx.doi.org/10.33019/aquatropica.v6i1.2458.
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Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.
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Rinto, Rinto, Ratih Dewanti, Sedarnawati Yasni, and Maggy Thenawidjaja Suhartono. "ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT PENGHASIL INHIBITOR ENZIM HMG-KoA REDUKTASE DARI BEKASAM SEBAGAI AGEN PEREDUKSI KOLESTEROL." Jurnal Agritech 35, no. 03 (October 6, 2015): 309. http://dx.doi.org/10.22146/agritech.9342.
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The purpose of this research was to obtain statins producer bacteria as a HMG-CoA reductase (HMGR) enzyme inhibitor to reduced cholesterol biosynthesis. Stages of this research were the isolation of compactin and lovastatin resistant bacteria, statin production, analysis of culture extracts to inhibition of HMG-CoA reductase and identification of bacteria. The results showed that the 20 isolates of compactin and lovastatin resistant bacteria, there are 5 bacterial isolates produced statins. They were L3.3.4; C3.4.2; C3.3.5; C3.4.4 and L3.3.3; with the statins content were 9.491;1.536; 0.065; 0.060; and 0.040 ppm. Selection of the 5 bacterial isolates resulted 2 bacteria which had inhibition ability to HMGR enzyme activity. They were Lactobacillus acidophilus and Lactobacillus delbruckii sp. delbruckii with inhibitory ability were 66.67% and 58.33%, respectively.Keywords: L. acidophilus, L. delbrucki, HMGR inhibitor, cholesterol, bekasamABSTRAKPenelitian ini bertujuan memperoleh bakteri penghasil statin sebagai inhibitor enzim HMG-KoA reduktase (HMGR), penghambat sintesis kolesterol. Tahapan penelitian yang dilakukan adalah isolasi bakteri yang resisten terhadap compactin dan lovastatin, produksi statin, uji penghambatan ekstrak dari kultur bakteri terhadap HMG-KoA reduktasedan identifikasi bakteri. Hasil penelitian menunjukan bahwa dari 20 isolat bakteri yang resisten terhadap compactin maupun lovastatin, terdapat 5 isolat bakteri yang potensial menghasilkan statin, yaitu isolat L3.3.4; C3.4.2; C3.3.5; C3.4.4 dan L3.3.3; dengan kandungan statin berturut-turut adalah 9.491; 1,536; 0,065, 0,060, dan 0,040 ppm. Seleksiterhadap 5 isolat menghasilkan 2 bakteri yang mempunyai kemampuan penghambatan terhadap aktivitas enzim ¸Â•ÝWtu Lactobacillus acidophilus dan Lactobacillus delbruckii sp. delbruckii dengan kemampuan penghambatan berturut-turut adalah 66,67% dan 58,33%.Kata kunci: L. acidophilus, L. delbrucki, inhibitor HMGR, kolesterol, bekasam
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Mozumder, N. H. M. R., M. Akhtaruzzaman, M. A. Bakr, and F. Tuj Zohra. "Study on Isolation and Partial Purification of Lactase (β-galactosidase) Enzyme from Lactobacillus Bacteria Isolated from Yogurt." Journal of Scientific Research 4, no. 1 (December 26, 2011): 239. http://dx.doi.org/10.3329/jsr.v4i1.8478.
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Lactase has many applications in dairy industry including for the treatment of lactose intolerance. The present study was conducted to identify the activity of lactase enzyme produced by Lactobacillus bacteria isolated from yogurts available in Dhaka city. The strains were identified to be gram positive, catalase negative, fermentative and lactase producer when cultured on selective MRS agar media by using standard bacteriological procedures and techniques. The study revealed that enzymes produced by lactobacilli were capable to produce glucose from substrate lactose in lactose modified media using lactase assay Kit Glu IB and their highest protein concentration (17.25 mg/ml) was observed in the supernatant of culture media isolated from L. lactis. Highest total activity (850.69 U/l) and specific activity (50.04 U/mg) of lactase enzyme was observed in the strain of L. bulgaricus. The crude extract which showed highest activity was further purified by ammonium sulphate precipitation followed by anion exchange column chromatography (DEAE cellulose). Final specific activity and fold purification of lactase enzyme reached to 62.80 U/mg and 1.47 respectively. The highest physic-chemical properties (Effect of pH and temperature) of lactase enzyme were observed at PH 6.0 which was 43.98 U/mg of protein and at 70°c temperature which was 111.11 U/mg of protein.Keywords: β-Galactosidase; Specific activity; DEAE cellulose; Fold purification; Yogurt.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.8478J. Sci. Res. 4 (1), 239-249 (2012)
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Pinyaphong, Porntippa, and Pensiri Sriburee. "Isolation of Bacteria from Clarifier Effluent of Sour Pork Industry Able to Produce 1,3-Propanediol from Crude Glycerol." Applied Mechanics and Materials 855 (October 2016): 37–41. http://dx.doi.org/10.4028/www.scientific.net/amm.855.37.
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The objective of this research was to isolate bacteria able to utilize glycerol as carbon source and study the optimal condition of 1,3-propanediol production from crude glycerol. The bacteria B-2, B-4 and B-7 could grow and produce 1,3-propanediol in basal medium formula 1,2 and 3 that containing glycerol as energy and carbon sources. The bacteria B-2 produced highest 1,3-propanediol in basal medium formula 2. In addition, the various factors that influenced to crude glycerol fermentation in basal medium formula 2 by the bacteria B-2 were investigated. It was found that the optimal conditions of 1,3-propanediol production were 10% bacterial inoculums and crude glycerol about 50 g/L was added. pH of basal medium was adjusted around 7 and the bacterial culture was incubated at 30°C with shaking at 100 rpm for 3 days. The highest yield of 1,3-propanediol was obtained about 0.4167 g/L.
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Viju, Nadarajan, Nagarajan Ezhilraj, Chellamnadar Vaikundavasagom Sunjai Shankar, Stanislaus Mary Josephine Punitha, and Sathianeson Satheesh. "Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)." Nova Biotechnologica et Chimica 17, no. 2 (December 1, 2018): 115–24. http://dx.doi.org/10.2478/nbec-2018-0012.
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AbstractBacteria associated with surfaces have been frequently cited as a potential source for the isolation of bioactive metabolites. In this study, bacteria associated with marine gastropod, Babylonia sp. were isolated and screened for antibacterial activity against biofilm-forming bacteria. The antibiofilm and antifouling effect of the selected surface- associated bacterial strains were examined under in vitro and in vivo conditions. Results showed that the extracellular polymeric substances (EPS) of the bacterial strain CML associated with gastropod species considerably reduced the adhesion of biofilm-forming bacteria on glass coupons. Besides, the antifouling coat prepared by incorporating of this EPS into polyurethane varnish prevented the settlement of biofoulers on test substratum submerged in marine waters. The functional groups present in the EPS were analyzed using FT-IR. The bacterium responsible for the production of the bioactive EPS was identified as Bacillus subtilis subsp. by 16S rRNA gene sequencing. More detailed characterization of the identified bioactive EPS could lead to the isolation of a novel natural antifouling product.
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Ahsan, Sunjukta, Rayhan Mahmud, Kajal Ahsan, and Shamima Begum. "Isolation of Multidrug Resistant Bacteria from Aspirates of Cancer Patients." Bangladesh Journal of Microbiology 35, no. 1 (January 15, 2019): 61–66. http://dx.doi.org/10.3329/bjm.v35i1.39805.
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Infections due to Gram-negative bacteria are common affairs in cancer patients during aggressive therapy. The present study characterizedmulti-drug resistant bacteria (MDR) isolated from cancer aspirates collected from patients admitted to the National Cancer Hospital in Dhaka, Bangladesh. A total of 210 aspirate samples were collected from cancer patients. Out of 210 samples Acinetobacter spp.led the list of isolates (8.89%, n=45). Of these species, 50% exhibited resistance to Amoxycillin and Nitrofurantoin, each, 25% exhibited resistant to Cefotaxime, Azithromycin, Ciprofloxacin, Clindamycin, and Sulfamethoxazole. A total of 33.33% of the Bordetella spp.which accounted 6.67%of the total isolates exhibited resistance to Cefotaxime. All oftheLegionellapneumophila,comprising 4.4%of the isolated species, wereresistant to Cefotaxime, Azithromycin, and Clindamycin.In contrast, 50% were resistant to Cefotaxime, Azithromycin, and Ceftriaxone. Of the Escherichia coli(4.4%, n=45) isolated,50% exhibited resistance to Cefotaxime, Clindamycin, Ceftriaxone, Amoxycillinand Sulfamethoxazole.The only isolate of Klebsiella sp. was demonstrated to be an ESBL producer. The isolation of multidrug resistant bacteria from cancer patients is of particular concern in Bangladesh where cancer and drug resistance are both common phenomena but treatment facilities are poor. To our knowledge this is the first report of the isolation of drug resistant bacteria from cancer patients from Dhaka city.
Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 61-66
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R Wan Abdul Razak, W., N. J Mohamad Yusuf, A. Abdul-Aziz, S. K Navaratnam, I. Zubir, and E. E Rizlan Ross. "Screening and Isolation of Polyhydroxyalkanoates (PHA)-Producing Bacteria from Landfill by using Cocoa Pod Husks as Carbon Source." International Journal of Engineering & Technology 7, no. 4.14 (December 24, 2019): 51. http://dx.doi.org/10.14419/ijet.v7i4.14.27470.
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Polyhydroxyalkanoates (PHA) are bioplastics, produced by various bacteria as food and energy reservoir. PHA is an alternative for synthetic plastic because they are environmentally friendly and can be degraded naturally by microorganisms. One of the important factors for the growth of PHA producing bacteria is an excess of carbon supply. In order to reduce the overall cost of PHA production, a low cost pure substrate, which is cocoa pod husks (CPH) was used as a carbon source. The objectives of this study were to isolate and screen PHA producing bacteria from landfill samples which are leachate and soil, to identify the PHA producing bacteria by using morphological characterization and 16s rDNA gene sequencing and to determine the best percentage of CPH that can be used as a carbon source for PHA producing bacteria. PHA producing bacteria from leachate and soil from landfill in Jeram, Selangor were screened by using Nile Blue A staining method. Two potential PHA producers with the brightest fluorescence under UV light from each samples were isolated and characterized by using morphological and molecular identification. Results of morphological identification shows all bacterial isolates have a rod shape and have a capsule, three bacterial isolates (L4, S3, S5) have an endospore while the remaining does not have endospore (L1). Three out of four were Gram positive bacteria (L4, S3, S5) and the remaining was Gram negative bacteria (L1). These isolates were confirmed of their identity as K. pneumoniae (L1), B. cereus (L4 & S3) and B. toyonensis (S5) using 16s rDNA gene sequencing. Different concentration of CPH, which are 2% (w/v), 5% (w/v), 7% (w/v) and 10% (w/v) were used to study the best percentage of CPH that can be used as carbon source. PHA accumulation was the highest at 7% (w/v) for all bacterial species tested and lowest at 10% (w/v) CPH except for B. toyonensis. Therefore, K. pneumoniae, B. cereus and B. toyonensis which isolated from landfill show the ability to produce PHA and the used of 7% (w/v) cocoa pod husks as carbon source give the highest PHA accumulation.
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Maarisit, Ismariani, Esther D. Angkouw, Remy E. P. Mangindaan, Natalie D. C. Rumampuk, Henky Manoppo, and Elvy Like Ginting. "Isolation and Antibacterial Activity Test of Seagrass Epiphytic Symbiont Bacteria Thalassia hemprichii from Bahowo Waters, North Sulawesi." Jurnal Ilmiah PLATAX 9, no. 1 (June 29, 2021): 115. http://dx.doi.org/10.35800/jip.9.1.2021.34320.
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Seagrass is a higher plant and has the ability to produce bioactive compounds such as antibacterial. Seagrass is also a host to a variety of bacteria. Bacteria that live in the host will produce the same compounds as the host's body. The utilization of symbiotic bacteria with seagrasses as producers of bioactive compounds such as antibacterial can be used as a solution to reduce excessive seagrass uptake in nature. On the other hand, bacteria have the advantage of being fast and easy to grow and can be mass-produced and more economical. This study aims to isolate and test the antibacterial activity of the epiphytic bacteria of seagrass symbionts. Epiphytic bacteria of seagrass symbionts were grown on Nutrient Agar media directly in the field and bacterial isolation was carried out based on the morphological characteristics of the bacterial isolates. The antibacterial activity test was carried out using the disc method with the test bacteria Stapylococcus aureus, Streptococcus mutans, Escherichia coli, Salmonella thypi, and antibiotics as positive controls. The ability of bacteria to produce antibacterial was indicated by the formation of an inhibition zone around the paper disc containing the epiphytic bacteria of the seagrass symbiont T. hemprichii. A total of 3 isolates of epiphytic bacteria were isolated from T. hemprichii seagrass from Bahowo Waters, Tongkaina Village, Bunaken District, these isolates are namely Epifit 1, Epiphyte 2, and Epiphyte 3. Epiphyte 2 isolate had antibacterial activity against S. mutans, S. aureus, and S. thypi test bacteria, Epiphyte 3 isolate had antibacterial activity against S. mutans, and S. thypi test bacteria.Key words: Bacteria; Antibacterial; T. hemprichii; symbionts; BahowoAbstrakLamun merupakan tumbuhan tingkat tinggi dan memiliki kemampuan menghasilkan senyawa bioaktif seperti antibakteri. Lamun juga merupakan tempat hidup atau inang dari berbagai bakteri. Bakteri yang hidup pada inang akan menghasilkan senyawa yang sama dengan tubuh inangnya. Pemanfaatan bakteri yang bersimbiosis dengan lamun sebagai produsen senyawa bioaktif seperti antibakteri dapat dijadikan sebagai solusi dalam mengurangi pengambilan lamun yang berlebihan di alam. Dilain pihak, bakteri memiliki keunggulan karena pertumbuhan bakteri yang cepat dan mudah tumbuh, dapat diproduksi secara massal dan lebih ekonomis. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antibakteri dari bakteri epifit simbion lamun T. hemprichii dari Perairan Bahowo. Bakteri epifit simbion lamun ditumbuhkan pada media NA secara langsung di lapangan dan isolasi bakteri dilaksanakan berdasarkan karateristik morfologi isolat bakteri. Uji aktivitas bakteri dilakukan menggunakan metode cakram dengan bakteri uji S. aureus, S. mutans, E. coli, dan S. thypi dan antibiotik sebagai kontrol positif. Kemampuan bakteri menghasilkan antibakteri ditandai dengan terbentuknya zona hambat disekitar kertas cakram yang mengandung bakkteri epifit simbion lamun T. hemprichii. Sebanyak 3 isolat bakteri epifit berhasil diisolasi pada lamun T. hemprichii dari Perairan Bahowo, Kelurahan Tongkaina, Kecamatan Bunaken yaitu Epifit 1, Epifit 2, dan Epifit 3. Isolat epifit 3 memiliki aktivitas antibakteri terhadap bakteri uji S. thypi, isolat Epifit 2 terhadap bakteri uji S. mutans, S. aureus, dan S. thypi, isolat Epifit 3 terhadap bakteri uji S. mutans, dan S. thypi.Kata kunci: Bakteri; Antibakteri; T. hemprichii; Simbion; Bahowo
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Raina, Jean-Baptiste, Dianne Tapiolas, Cherie A. Motti, Sylvain Foret, Torsten Seemann, Jan Tebben, Bette L. Willis, and David G. Bourne. "Isolation of an antimicrobial compound produced by bacteria associated with reef-building corals." PeerJ 4 (August 18, 2016): e2275. http://dx.doi.org/10.7717/peerj.2275.
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Bacterial communities associated with healthy corals produce antimicrobial compounds that inhibit the colonization and growth of invasive microbes and potential pathogens. To date, however, bacteria-derived antimicrobial molecules have not been identified in reef-building corals. Here, we report the isolation of an antimicrobial compound produced byPseudovibriosp. P12, a common and abundant coral-associated bacterium. This strain was capable of metabolizing dimethylsulfoniopropionate (DMSP), a sulfur molecule produced in high concentrations by reef-building corals and playing a role in structuring their bacterial communities. Bioassay-guided fractionation coupled with nuclear magnetic resonance (NMR) and mass spectrometry (MS), identified the antimicrobial as tropodithietic acid (TDA), a sulfur-containing compound likely derived from DMSP catabolism. TDA was produced in large quantities byPseudovibriosp., and prevented the growth of two previously identified coral pathogens,Vibrio coralliilyticusandV. owensii, at very low concentrations (0.5 μg/mL) in agar diffusion assays. Genome sequencing ofPseudovibriosp. P12 identified gene homologs likely involved in the metabolism of DMSP and production of TDA. These results provide additional evidence for the integral role of DMSP in structuring coral-associated bacterial communities and underline the potential of these DMSP-metabolizing microbes to contribute to coral disease prevention.
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Ifandi, Slamet, and Muh Alwi. "Isolation of Thermophilic Bacteria from Bora Hot Springs in Central Sulawesi." Biosaintifika: Journal of Biology & Biology Education 10, no. 2 (August 29, 2018): 291–97. http://dx.doi.org/10.15294/biosaintifika.v10i2.14905.
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Thermophilic bacteria can survive at high temperature, in which hot spring is one of its habitats. Indonesia has many hot springs with potential as a habitat for thermophilic bacteria. The purpose of this study was to obtain isolates thermophilic bacteria from Bora hotspring located in Central Sulawesi. This study applied a descriptive-observational study design, characteristics of bacterial properties identified using conventional methods according to the Bergey’s Manual of Determinative Bacteriology. The study was conducted in 3 stages. The first stage was bacteria cultivation on the appropriate media, followed by stage of isolated and the last stage by identified characteristics of thermophilic bacteria which included microscopic and macroscopic morphology, Physiological and biochemistry test. The results of the isolates indicted 4 representative isolated of thermophilic bacteria from Bora Hot Spring namely TM022, TM023, TM024, TM026. The bacteria isolates obtained were bacillus, coccus and Gram negative and positive, while the physiological test of all isolates were able to grow and showed changes in the medium. This study is useful in providing characteristic of indigenous thermophilic bacteria isolates that produces thermostable enzymes.
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Aqueveque, Pedro, Timm Anke, and Olov Sterner. "The Himanimides, New Bioactive Compounds From Serpula Himantoides (Fr.)Karst." Zeitschrift für Naturforschung C 57, no. 3-4 (April 1, 2002): 257–62. http://dx.doi.org/10.1515/znc-2002-3-410.
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In a screening of basidiomycete cultures from Chile for the production of antibiotics we identified a Serpula himantoides strain as a producer of metabolites inhibiting the growth of bacteria and fungi. Bioactivity guided purification resulted in the isolation of four new antibiotics. Their structures were elucidated by spectroscopic methods. All four compounds are succinimide and maleimide derivatives, of which two are N-hydroxylated
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Huynh, Phong X. "Isolation and selection of microorganisms in cocoa fermentation." Journal of Agriculture and Development 18, no. 4 (August 30, 2019): 51–61. http://dx.doi.org/10.52997/jad.7.04.2019.
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The objectives of this study were to investigate the change of microorganisms involved in cocoa (Theobroma cacao) fermentation and then to isolate, characterize and select the important microorganisms in cocoa fermentation. The results showed that microbial quantities continuously changed during cocoa fermentation and the highest quantity of dominant microorganisms at different stages of fermentation process as 8.03 log cfu/g of yeast, 6.34 log cfu/g of mold, 7.77 log cfu/g of lactic acid bacteria, 7.87 log cfu/g of acetic acid bacteria, 7.25 log cfu/g of Bacillus, and 10.93 log cfu/g of the total aerobic bacteria. There were nine yeast isolates belonging 5 genera of Saccharomyces, Kluyveromyces, Brettanomyces, Candida and Cystofilobasidium; 9 mould isolates belonging to 2 genera of Rhizopus and Aspergillus; 11 acetic acid bacteria isolates belonging to Acetobacter; and 13 spore-forming bacterial isolates belonging to Bacillus isolates. Three isolates of yeast (CY-1a, CY-1b, CY-2a) belonging to Kluyveromyces possessed the high fermentative capacity and 4 Acetobacter isolates (CAAB-1d, CAAB-1a, CAAB-1e and CAAB-2d) produced high amounts of acetic acid.
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Kurniawan, Ardiansyah, Suci Puspita Sari, Euis Asriani, Andi Kurniawan, Abu Bakar Sambah, and Asep Awaludin Prihanto. "Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka." International Journal of Applied Biology 3, no. 1 (June 29, 2019): 7. http://dx.doi.org/10.20956/ijab.v3i1.5848.
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Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.
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Sepriana, Citra, and Eti Sumiati. "Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen." Jurnal Penelitian Pendidikan IPA 6, no. 1 (January 29, 2020): 101. http://dx.doi.org/10.29303/jppipa.v6i1.340.
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This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.
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Cho, Hye-sun, Munjeong Choi, Yunjung Lee, Hyoim Jeon, Byeongyong Ahn, Nagasundarapandian Soundrarajan, Kwonho Hong, Jin-Hoi Kim, and Chankyu Park. "High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide." International Journal of Molecular Sciences 22, no. 8 (April 16, 2021): 4149. http://dx.doi.org/10.3390/ijms22084149.
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The efficiency of existing cell lysis methods to isolate nucleic acids from diverse bacteria varies depending on cell wall structures. This study tested a novel idea of using broad-spectrum antimicrobial peptides to improve the lytic efficiency of hard-to-lyse bacteria and characterized their differences. The lysis conditions of Staphylococcus aureus using recombinant porcine myeloid antimicrobial peptide 36 (PMAP-36), a broad-spectrum pig cathelicidin, was optimized, and RNA isolation was performed with cultured pellets of ten bacterial species using various membranolytic proteins. Additionally, three other antimicrobial peptides, protegrin-1 (PG-1), melittin, and nisin, were evaluated for their suitability as the membranolytic agents of bacteria. However, PMAP-36 use resulted in the most successful outcomes in RNA isolation from diverse bacterial species. The amount of total RNA obtained using PMAP-36 increased by ~2-fold compared to lysozyme in Salmonella typhimurium. Streptococci species were refractory to all lytic proteins tested, although the RNA yield from PMAP-36 treatment was slightly higher than that from other methods. PMAP-36 use produced high-quality RNA, and reverse transcription PCR showed the efficient amplification of the 16S rRNA gene from all tested strains. Additionally, the results of genomic DNA isolation were similar to those of RNA isolation. Thus, our findings present an additional option for high quality and unbiased nucleic acid isolation from microbiomes or challenging bacterial strains.
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Franco, Wendy, Ilenys Pérez-Díaz, Lauren Connelly, and Joscelin Diaz. "Isolation of Exopolysaccharide-Producing Yeast and Lactic Acid Bacteria from Quinoa (Chenopodium Quinoa) Sourdough Fermentation." Foods 9, no. 3 (March 13, 2020): 337. http://dx.doi.org/10.3390/foods9030337.
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Quinoa, a nutritional grain, can be used as an ingredient in gluten-free sourdoughs. This study characterizes quinoa flour spontaneous fermentation with emphasis in the isolation of exopolysaccharide (EPS) producer bacteria. Real, red and black grains were studied. Dough yield, microbiota composition and fermentation biochemistry were determined for a total of 36 quinoa flour fermentations. The fermentation biochemistry was monitored by high-performance liquid chromatography (HPLC) analysis, pH measurement and titratable acidity. Changes in the microbiota were monitored by plating on deMann Rogosa and Sharp 5 agar (MRS5) and yeast and mold agar (YMA) plates and with metagenetic analysis. The ability to produce exopolysaccharides was screened in selected lactic acid bacteria (LAB) isolates. Production of organic acids in the spontaneous fermentation dropped the pH to 4.0 ± 0.3. The community of presumptive LAB reached 8.37 ± 0.01 log colony forming units (CFU)/mL by day 8 of back-slopped fermentations. The microbiota was composed of Lactobacillus, Enterococcus, Leuconostoc, Lactococcus, Pediococcus and Weissella. P. pentosaceous, L. citreum and W. cibaria were able to produce EPS in a starch-rich medium. P. pentosaceous showed higher exopolysaccharide yield, rapid acidifying kinetics and was able to drop the dough broth pH to values below 4.0 and a positive fermentation quotient after 24 h of incubation. Therefore, the bacterium might be a potential candidate for quinoa sourdough production.
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Luna, Emily, Leon van Eck, Tony Campillo, Margaret Weinroth, Jessica Metcalf, Alvaro L. Perez-Quintero, Anna-Maria Botha, et al. "Bacteria Associated with Russian Wheat Aphid (Diuraphis noxia) Enhance Aphid Virulence to Wheat." Phytobiomes Journal 2, no. 3 (January 2018): 151–64. http://dx.doi.org/10.1094/pbiomes-06-18-0027-r.
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Phenotypic responses to biotic stresses are often studied as the interactions between two species; however, in the phytobiome, these responses frequently result from complex interactions involving several organisms. Here, we show that variation in chlorosis caused by Russian wheat aphid (Diuraphis noxia) feeding is determined, in part, by aphid-associated bacteria. Proteomic analysis of fluids injected into a sterile medium by the aphid during feeding indicate that 99% of the proteins are of bacterial origin. Of these, the greatest proportion are produced by bacteria in the order Enterobacteriales. Bacteria from five genera in four families that have the capacity to produce these proteins were isolated directly from aphids as well as from wheat leaves only after D. noxia feeding. By themselves or in combination, these bacteria were not virulent to wheat, even at high inoculum levels. Metagenomic analysis showed that the same five D. noxia-associated genera dominated the non-Buchnera component of the aphid microbiome, and that representation of these genera was reduced in aphids from colonies established after isolation of newborn nymphs from their mothers prior to feeding (isolated aphids). Isolation or treatment with antibiotics reduced bacterial numbers, and these aphids caused less feeding damage on wheat than non-isolated or non-antibiotic-treated aphids. Our data show that bacterial proteins are a significant component of Russian wheat aphid saliva, that the bacteria producing these proteins are associated with aphids and plants fed upon by aphids, and that these aphid-associated bacteria facilitate aphid virulence to wheat.
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Kim, A.-Leum, Seunghye Park, Yoon-Kyoung Hong, Ji-Hwan Shin, and Se-Hwan Joo. "Isolation and Characterization of Beneficial Bacteria from Food Process Wastes." Microorganisms 9, no. 6 (May 27, 2021): 1156. http://dx.doi.org/10.3390/microorganisms9061156.
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Significant quantities of food waste are accumulated globally on an annual basis, with approximately one-third of the food produced (equivalent to 1.3 billion tons of food) being wasted each year. A potential food waste recycling application is its utilization as a soil conditioner or fertilizer, whereby it increases the soil organic content and microbial biomass. This study evaluated the effectiveness of food waste as a microbial resource by analyzing the microbial community composition and isolating plant growth-promoting bacteria (PGPB) in food waste obtained from various sources. High-throughput sequencing identified 393 bacterial operational taxonomic units in the food process waste (FPW) samples. Moreover, the results showed that Firmicutes was abundant in the waste samples, followed by Bacteroidetes and Proteobacteria. A total of 92 bacteria were isolated from FPW. Moreover, the cultivable strains isolated from FPW belonged to the genus Bacillus, followed by Streptomyces and Proteus. Six isolated bacteria exhibited beneficial traits, including indole acetic acid production, antifungal resistance and extracellular lysis. FPW is a valuable microbial resource for isolation of PGPB, and its use as a fertilizer may enable a reduction in chemical fertilizer usage, thereby mitigating the corresponding adverse environmental impacts on sustainable crop development.
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Yang, Xu, En Huang, Chunhua Yuan, Liwen Zhang, and Ahmed E. Yousef. "Isolation and Structural Elucidation of Brevibacillin, an Antimicrobial Lipopeptide from Brevibacillus laterosporus That Combats Drug-Resistant Gram-Positive Bacteria." Applied and Environmental Microbiology 82, no. 9 (February 26, 2016): 2763–72. http://dx.doi.org/10.1128/aem.00315-16.
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ABSTRACTA new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistantStaphylococcus aureus, vancomycin-resistant strains ofEnterococcus faecalisandLactobacillus plantarum, and other Gram-positive bacteria. The producer strain, designated OSY-I1, was determined to beBrevibacillus laterosporusvia morphological, biochemical, and genetic analyses. The antimicrobial agent was extracted from cells of OSY-I1with isopropanol, purified by high-performance liquid chromatography, and structurally analyzed using mass spectrometry (MS) and nuclear magnetic resonance (NMR). The MS and NMR results, taken together, uncovered a linear lipopeptide consisting of 13 amino acids and an N-terminal C6fatty acid (FA) chain, 2-hydroxy-3-methylpentanoic acid. The lipopeptide (FA-Dhb-Leu-Orn-Ile-Ile-Val-Lys-Val-Val-Lys-Tyr-Leu-valinol, where Dhb is α,β-didehydrobutyric acid and valinol is 2-amino-3-methyl-1-butanol) has a molecular mass of 1,583.0794 Da and contains three modified amino acid residues: α,β-didehydrobutyric acid, ornithine, and valinol. The compound, designated brevibacillin, was determined to be a member of a cationic lipopeptide antibiotic family. In addition to its potency against drug-resistant bacteria, brevibacillin also exhibited low MICs (1 to 8 μg/ml) against selected foodborne pathogenic and spoilage bacteria, such asListeria monocytogenes,Bacillus cereus, andAlicyclobacillus acidoterrestris. Purified brevibacillin showed no sign of degradation when it was held at 80°C for 60 min, and it retained at least 50% of its antimicrobial activity when it was held for 22 h under acidic or alkaline conditions. On the basis of these findings, brevibacillin is a potent antimicrobial lipopeptide which is potentially useful to combat drug-resistant bacterial pathogens and foodborne pathogenic and spoilage bacteria.
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Kamallia, Sonia, M. Hasbi, and Budijono Budijono. "Isolation and Identification of Biosurfactant Producing Bacteria from Tofu Liquid Waste UD. Dika Putra, Riau Province." Ilmu Perairan (Aquatic Science) 9, no. 1 (March 30, 2021): 16. http://dx.doi.org/10.31258/jipas.9.1.p.16-22.
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Tofu liquid waste contains high levels of organic matter, especially protein and amino acids. These organic compounds can be proteins, carbohydrates and fats. Most of the bacteria are able to use oil or fat as a source of carbon and energy, bacteria that have this ability are often known as lipolytic bacteria. This study aims to obtain biosurfactant producing bacteria from tofu wastewater. This research was conducted from July - September 2020. The method used in this research is survey method and emulsification method. The media used for bacterial isolation were Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA). 6 of the isolates are able to produce biosurfactants. Morphological and biochemical characteristics indicate six bacterial genera, namely Genus Agrobacterium, Proteus, Proteus, Citrobacter, Enterobacter, and Serratia. The bacteria obtained are bacteria that have the potential to degrade oil in a polluted environment.