Academic literature on the topic 'Production of lipase'

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Dissertations / Theses on the topic "Production of lipase"

1

Salgueiro, Alexandra Amorim. "Lipase production in Candida lipolytica 1055." Thesis, University of St Andrews, 1994. http://hdl.handle.net/10023/14328.

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Microbial lipases are now increasingly employed in the redesign of animal fats and vegetable oils. Commercial scale manufacture of microbial lipases, which is limited, tends to be surrounded by secrecy and much remains to be clarified in terms of optimization of fermentation conditions. C. lipolytica metabolizes cheap carbon sources (from wastes and by-products), in particular non- carbohydrate substrates, accumulating lipid during the growth. As all potent producers of lipase are also amongst the lipid-accumulating microorganisms, this yeast shows potential as a major lipase producer. The fut
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2

Smith, Catherine J. "Production of a lipase from a Pseudomonad species." Thesis, Cranfield University, 1991. http://dspace.lib.cranfield.ac.uk/handle/1826/4183.

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A lipase-producing micro-organism was found to be a strain of Pseudomonas aeruginosa. When it was grown batchwise in a stirred 2 dM3 fermenter in a simple defined minimal salts medium containing yeast extract and glucose, it produced lipase at a level of 0.173 LU/cm3 without the need for a lipid substrate. Batch cultures of cells were fed glucose at constant rates. An optimum rate was found to be 0.50 g/dm3/h and this produced a maximum lipase activity of 0.80 LU/cm3. Medium composition was changed until it was possible to produce 25 g/dm3 dry weight of cells. Evidence of linear growth due to
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3

Ventura, Sónia Patrícia Marques. "Production of lipase by extractive fermentation with ionic liquids." Doctoral thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7023.

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Doutoramento em Engenharia Química<br>Novos processos fermentativos, designados por processos de Fermentação Extractiva, são caracterizados por apresentarem etapas de produção e extracção em simultâneo. A extracção líquido-líquido como técnica de separação é amplamente usado na indústria química pela sua simplicidade, baixo custo e facilidade de extrapolação de escala. No entanto o uso de solventes orgânicos nestes processos potencia os riscos ocupacionais e ambientais. Neste contexto, o uso de sistemas de duas fases aquosas baseados em líquidos iónicos, apresenta-se como uma técnica ef
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4

Lee, Seoung Yong. "Production and characterization of esterase-lipase from Lactobacillus casei subspecies." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61842.

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5

Smerdon, Gary Randall. "Production of human gastric lipase in the fission yeast Schizosaccharomyces pombe." Thesis, University of Exeter, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261145.

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6

Rodrigues, Roberta da Silva Bussamara. "Produção e caracterização de um biocatalisador heterogêneo para ser utilizado em aplicações industriais." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/17834.

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Nesse trabalho foram produzidas lipases da levedura Pseudozyma hubeiensis (HB85A) em reator de 14 L. Após produção da enzima, a lipase foi imobilizada por adsorção em suporte hidrofóbico por processo contínuo em reator de leito fixo. As melhores condições de imobilização foram: tempo de imobilização de 2 h e 29 min., pH de 4,76 e quantidade de enzima livre adicionada por grama de suporte de 1282 U/ g de suporte, sendo que, a máxima atividade da lipase imobilizada obtida foi de 143 U/g de suporte. O sobrenadante contendo lipase e o biocatalisador heterogênio foram caracterizados por planejament
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7

McCarthy, Conor Neil, and n/a. "Regulatory Elements Controlling Lipase and Metalloprotease Production in Pseudomonas fluorescens B52." Griffith University. School of Health Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20031015.124744.

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Psychrotrophic bacteria, such as Pseudomonas fluorescens B52, are a major cause of milk spoilage at refrigeration temperature due to the production of lipolytic and proteolytic enzymes. Regulatory mechanisms controlling the production of lipase and protease by the B52 lipA and aprX genes were investigated. Transposon mutagenesis identified the possible involvement of a poly-A polymerase enzyme which destabilises mRNA by 3' polyadenylation. A homologue of the E. coli EnvZ/OmpR two-component sensor/regulator system was identified by transposon mutagenesis and shown to repress lipase and protease
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8

KERBER, TATIANA DE MATTOS. "EXPERIMENTAL DESIGN AND METROLOGICAL EVALUATION OF LIPASE PRODUCTION BY YARROWIA LIPOLYTICA." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2007. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=10700@1.

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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO<br>Objetivo: Otimização da produção de lipases por células de Yarrowia lipolytica, através do planejamento experimental e avaliação dos aspectos metrológicos envolvidos no processo. Motivação: Embora existam muitos trabalhos na literatura que relatem a produção de lipases por microrganismos, poucos abordam a otimização do processo através do planejamento experimental e nenhum deles considera o rigor metrológico e a estimativa da incerteza de medição. Contextualização: As enzimas lipases serão objeto de estudo por apresentarem grande ver
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9

May, Michael 1972. "Production of Lipase by Candida bombicola in a self-cycling fermenter (SCF)." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27242.

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The Self-Cycling Fermentation (SCF) technique and an agar diffusion assay were used to follow the production of lipase by Candida bombicola. Repeatable lipase production profiles were determined for two different media. Each medium gave a considerably different profile.<br>When the broth contained oil, 60 to 80% of the lipase activity remained after freezing. All activity was lost when the broth did not contain oil.<br>The lipase was found to be associated with the cell. Up to a certain concentration of lipase, the presence of agar caused the lipase to disassociate from the cell and diffuse in
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10

Aryee, Alberta. "Immobilization of lipase and biodiesel production from fishery and animal processing waste." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110391.

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Biodiesel (BD) is conventionally produced by the transesterification of vegetable oils or animal fats with a monohydric alcohol and base catalyst, and known for its many technical and environmental advantages over petrodiesel. However, these oils are not economically viable feedstock due to their value as edible oils. Alternative, inexpensive feedstocks with minimal to no food value such as the large quantities of fishery and animal processing by-products which are often discarded as waste were explored in this project. Lipase was investigated as an alternative to chemical catalyst due to the
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