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1

Shock, Everett L. "Catalysing methane production." Nature 368, no. 6471 (April 1994): 499–500. http://dx.doi.org/10.1038/368499a0.

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2

Xin, Jia-ying, Jun-ru Cui, Jian-zhong Niu, Shao-feng Hua, Chun-gu Xia, Shu-ben Li, and Li-min Zhu. "Production of methanol from methane by methanotrophic bacteria." Biocatalysis and Biotransformation 22, no. 3 (May 2004): 225–29. http://dx.doi.org/10.1080/10242420412331283305.

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3

Wilkinson, J. M. "Methane production by ruminants." Livestock 17, no. 4 (July 2012): 33–35. http://dx.doi.org/10.1111/j.2044-3870.2012.00125.x.

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4

Minami, K. "Methane from rice production." Fertilizer Research 37, no. 3 (1994): 167–79. http://dx.doi.org/10.1007/bf00748935.

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5

Haitl, Martina, Tomáš Vítěz, Tomáš Koutný, Radovan Kukla, Tomáš Lošák, and Ján Gaduš. "Use of G-phase for biogas production." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 60, no. 6 (2012): 89–96. http://dx.doi.org/10.11118/actaun201260060089.

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Biogas is very promising renewable energy resource. The number of biogas plants increase every year. Currently there is a demand for new ways of organic waste treatment from production of different commodities. One of the technologies which produce waste is biodiesel production. One of the wastes from the biodiesel production is G-phase which is mainly consisted from glycerol and methanol. The aim of work was to find the effect of G-phase addition, to fermented material, on biogas resp. methane production. Two lab-scale batch anaerobic fermentation tests (hydraulic retention time 14 and 22 days) under mesophilic temperature conditions 38.5 °C have been performed. The positive effect of G-phase addition to methane production has been found. G-phase was added in three different amounts of inoculums volume 0.5 %, 1% and 1.5 %. The highest absolute methane production has been achieved by 1.5 % addition of G-phase. However it was also found difference in specific methane production due to use of different inoculum consisted of swine or cow manure. The specific methane production in hydraulic retention time of 14 days has been for the same G-phase dose 1.5 % higher for swine manure, 0.547 m3∙kg−1 of organics solids compare with cow liquid manure 0.474 m3∙kg−1 of organics solids.
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6

Mujiyo, M., B. H. Sunarminto, E. Hanudin, J. Widada, and J. Syamsiyah. "Methane production potential of soil profile in organic paddy field." Soil and Water Research 12, No. 4 (October 9, 2017): 212–19. http://dx.doi.org/10.17221/58/2016-swr.

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The use of organic fertilizers in the organic paddy/rice field can increase methane (CH<sub>4</sub>) production, which leads to environmental problems. In this study, we aimed to determine the CH<sub>4</sub> production potential (CH<sub>4</sub>-PP) by a soil profile from samples using flood incubation. Soil properties (chemical, physical, and biological) were analyzed from soil samples of three different paddy farming systems (organic, semi-organic, and conventional), whilst soil from teak forest was used as the control. A significant relationship was determined between soil properties and CH<sub>4</sub>-PP. The average amount of CH<sub>4</sub>-PP in the organic rice field profile was the highest among all the samples (1.36 µg CH<sub>4</sub>/kg soil/day). However, the CH<sub>4</sub> oxidation potential (CH<sub>4</sub>-OP) is high as well, as this was a chance of mitigation options should focus on increasing the methanotrophic activity which might reduce CH<sub>4</sub> emissions to the atmosphere. The factor most influencing CH<sub>4</sub>-PP is soil C-organic (C<sub>org</sub>). C<sub>org</sub> and CH<sub>4</sub>-PP of the top soil of organic rice fields were 2.09% and 1.81 µg CH<sub>4</sub>/kg soil/day, respectively. As a consequence, here the mitigation options require more efforts than in the other farming systems. Soil with various amounts of C<sub>org</sub> reached a maximum point of CH<sub>4</sub>-PP at various time after incubation (20, 15, and 10 days for the highest, medium, and the lowest amounts of C<sub>org</sub>, respectively). A high amount of C<sub>org</sub> provided enough C substrate for producing a higher amount of CH<sub>4</sub> and reaching its longer peak production than the low amount of C<sub>org</sub>. These findings also provide guidance that mitigation option reduces CH<sub>4 </sub>emissions from organic rice fields and leads to drainage every10–20 days before reaching the maximum CH<sub>4</sub>-PP.
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7

Patel, Sanjay K. S., Jae-Hoon Jeong, Sanjeet Mehariya, Sachin V. Otari, Bharat Madan, Jung Rim Haw, Jung-Kul Lee, Liaoyuan Zhang, and In-Won Kim. "Production of Methanol from Methane by Encapsulated Methylosinus sporium." Journal of Microbiology and Biotechnology 26, no. 12 (December 28, 2016): 2098–105. http://dx.doi.org/10.4014/jmb.1608.08053.

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8

Kussmaul, Martin, Markus Wilimzig, and Eberhard Bock. "Methanotrophs and Methanogens in Masonry." Applied and Environmental Microbiology 64, no. 11 (November 1, 1998): 4530–32. http://dx.doi.org/10.1128/aem.64.11.4530-4532.1998.

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ABSTRACT Methanotrophs were present in 48 of 225 stone samples which were removed from 19 historical buildings in Germany and Italy. The average cell number of methanotrophs was 20 CFU per g of stone, and their activities ranged between 11 and 42 pmol of CH4 g of stone−1 day−1. Twelve strains of methane-oxidizing bacteria were isolated. They belonged to the type II methanotrophs of the genera Methylocystis,Methylosinus, and Methylobacterium. In masonry, growth substrates like methane or methanol are available in very low concentrations. To determine if methane could be produced by the stone at rates sufficient to support growth of methanotrophs, methane production by stone samples under nonoxic conditions was examined. Methane production of 0.07 to 215 nmol of CH4 g of stone−1 day−1 was detected in 23 of 47 stone samples examined. This indicated the presence of the so-called “mini-methane”-producing bacteria and/or methanogenic archaea. Methanotrophs occurred in nearly all samples which showed methane production. This finding indicated that methanotrophs depend on biogenic methane production in or on stone surfaces of historical buildings.
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9

Domashenko, A. M., A. L. Dovbish, R. V. Darbinyan, A. I. Lyapin, and V. A. Peredel'skii. "Analysis of Liquefied Methane Production Technology Depending on Methane Purity and Production Volume." Chemical and Petroleum Engineering 40, no. 3/4 (March 2004): 145–48. http://dx.doi.org/10.1023/b:cape.0000033665.68451.1c.

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10

Zhou, Yue, Marco J. Castaldi, and Tuncel M. Yegulalp. "Experimental Investigation of Methane Gas Production from Methane Hydrate." Industrial & Engineering Chemistry Research 48, no. 6 (March 18, 2009): 3142–49. http://dx.doi.org/10.1021/ie801004z.

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11

Harpalani, Satya, Basanta K. Prusty, and Pratik Dutta. "Methane/CO2Sorption Modeling for Coalbed Methane Production and CO2Sequestration." Energy & Fuels 20, no. 4 (July 2006): 1591–99. http://dx.doi.org/10.1021/ef050434l.

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12

Kunkel, Benny, Dominik Seeburg, Tim Peppel, Matthias Stier, and Sebastian Wohlrab. "Combination of Chemo- and Biocatalysis: Conversion of Biomethane to Methanol and Formic Acid." Applied Sciences 9, no. 14 (July 12, 2019): 2798. http://dx.doi.org/10.3390/app9142798.

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In the present day, methanol is mainly produced from methane via reforming processes, but research focuses on alternative production routes. Herein, we present a chemo-/biocatalytic oxidation cascade as a novel process to currently available methods. Starting from synthetic biogas, in the first step methane was oxidized to formaldehyde over a mesoporous VOx/SBA-15 catalyst. In the second step, the produced formaldehyde was disproportionated enzymatically towards methanol and formic acid in equimolar ratio by formaldehyde dismutase (FDM) obtained from Pseudomonas putida. Two processing routes were demonstrated: (a) batch wise operation using free formaldehyde dismutase after accumulating formaldehyde from the first step and (b) continuous operation with immobilized enzymes. Remarkably, the chemo-/biocatalytic oxidation cascades generate methanol in much higher productivity compared to methane monooxygenase (MMO) which, however, directly converts methane. Moreover, production steps for the generation of formic acid were reduced from four to two stages.
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13

Adamovics, Aleksandrs M., Semjons A. Ivanovs, and Vilis S. Dubrovskis. "Methane Production From Industrial Hemp." Agricultural Machinery and Technologies 13, no. 2 (April 28, 2019): 20–26. http://dx.doi.org/10.22314/2073-7599-2018-13-2-20-26.

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Abstract. Due to the increasing shortage of fossil fuels, the use of alternative energy sources is becoming even more popular. In Latvia, maize is predominantly used for the production of biogas, and other crops are being studied for this purpose. (Research purpose) To study the productivity of industrial hemp varieties (Cannabis sativa L.) and the possibility of obtaining biogas from hemp. (Materials and methods) Field experiments on hemp productivity were carried out on sod calcareous, heavy dusty sand clay soils in 2012-2014. Ten industrial varieties of hemp – 'Bialobrzeskie', 'Futura 75', 'Fedora 17', 'Santhica 27', 'Beniko', 'Ferimon', 'Epsilon 68', 'Tygra', 'Wojko', and 'Uso 31' – were sown with a seeding rate of 50 kilogram per hectare at the background of fertilizers: nitrogen – 120, phosphoric oxide – 90, potassium oxide – 150 kilogram per hectare. Hemp was sown on 10-square meter plots in mid-May, in triplicate. Hemp was harvested at the beginning of seed ripening phase. The whole crop of green mass was calculated on a completely dry matter. The fermentation process for the production of biogas, the average yield of methane, and other parameters were studied in the Laboratory of Bioenergetics of the Latvia University of Life Sciences and Technologies, using small-sized bioreactors. (Results and discussion) The dry matter yield of hemp obtained in the agro-climatic conditions of Latvia averaged 13.32- 17.78 tons per hectare. For an average of three years (2012-2014), higher yields of dry matter were obtained from the varieties of 'Futura 75' (17.76 tons per hectare) and 'Tygra’ (16.31 tons per hectare). The average amount of methane obtained from the 'Uso 31' leaves was 0.365 litre from one gramme of dry organic matter, which is a very good result as compared to other energy crops, for example, corn silage (0.319-0.330 litre from one gramme of dry organic matter in Latvia). (Conclusions) The research has demonstrated that hemp can be successfully used to produce biogas, and hemp leaves are the most suitable starting material.
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14

Peled, Y. "Methane Production and Colon Cancer." Gastroenterology 88, no. 5 (May 1985): 1294. http://dx.doi.org/10.1016/s0016-5085(85)80111-6.

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15

Hill, Julian, Chris McSweeney, André-Denis G. Wright, Greg Bishop-Hurley, and Kourosh Kalantar-zadeh. "Measuring Methane Production from Ruminants." Trends in Biotechnology 34, no. 1 (January 2016): 26–35. http://dx.doi.org/10.1016/j.tibtech.2015.10.004.

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16

Hackstein, J. H., and C. K. Stumm. "Methane production in terrestrial arthropods." Proceedings of the National Academy of Sciences 91, no. 12 (June 7, 1994): 5441–45. http://dx.doi.org/10.1073/pnas.91.12.5441.

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17

Yan, J. Q., P. H. Liao, and K. V. Lo. "Methane production from cheese whey." Biomass 17, no. 3 (January 1988): 185–202. http://dx.doi.org/10.1016/0144-4565(88)90113-8.

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18

Ghanem, K. M., A. H. El-Refai, and M. A. El-Gazaerly. "Methane production from beet pulp." Resources, Conservation and Recycling 6, no. 3 (May 1992): 267–75. http://dx.doi.org/10.1016/0921-3449(92)90036-2.

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19

Grossart, Hans-Peter. "Methane Production in Oxic Environments." Video Proceedings of Advanced Materials 2, no. 2 (April 1, 2021): 2021–02118. http://dx.doi.org/10.5185/vpoam.2021.02118.

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20

Michalkiewicz, Beata. "Assessment of the possibility of the methane to methanol transformation." Polish Journal of Chemical Technology 10, no. 2 (January 1, 2008): 20–26. http://dx.doi.org/10.2478/v10026-008-0023-5.

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Assessment of the possibility of the methane to methanol transformation The methane to methanol conversion via esterification is an interesting method which makes it possible to eliminate the otherwise necessary phase of obtaining synthesis gas. On the basis of laboratory investigations mass balances for this process were determined. Preliminary assessment of the way of conducting the process and possibilities of practical applications of this technology was also made. It was pointed out that regardless of any possible modifications of methane to methanol conversion via esterification redundant sulfuric acid will always be produced during ester hydrolysis. Production of methanol from methane using this method can only be done when it is combined with producing other substances, which needs using H2SO4.
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21

Penger, Jörn, Ralf Conrad, and Martin Blaser. "Stable Carbon Isotope Fractionation by Methylotrophic Methanogenic Archaea." Applied and Environmental Microbiology 78, no. 21 (August 17, 2012): 7596–602. http://dx.doi.org/10.1128/aem.01773-12.

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ABSTRACTIn natural environments methane is usually produced by aceticlastic and hydrogenotrophic methanogenic archaea. However, some methanogens can use C1compounds such as methanol as the substrate. To determine the contributions of individual substrates to methane production, the stable-isotope values of the substrates and the released methane are often used. Additional information can be obtained by using selective inhibitors (e.g., methyl fluoride, a selective inhibitor of acetoclastic methanogenesis). We studied stable carbon isotope fractionation during the conversion of methanol to methane inMethanosarcina acetivorans,Methanosarcina barkeri, andMethanolobus zinderiand generally found large fractionation factors (−83‰ to −72‰). We further tested whether methyl fluoride impairs methylotrophic methanogenesis. Our experiments showed that even though a slight inhibition occurred, the carbon isotope fractionation was not affected. Therefore, the production of isotopically light methane observed in the presence of methyl fluoride may be due to the strong fractionation by methylotrophic methanogens and not only by hydrogenotrophic methanogens as previously assumed.
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22

Mudge, L. K., E. G. Baker, D. H. Mitchell, and M. D. Brown. "Catalytic Steam Gasification of Biomass for Methanol and Methane Production." Journal of Solar Energy Engineering 107, no. 1 (February 1, 1985): 88–92. http://dx.doi.org/10.1115/1.3267660.

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The technical and economic feasibility of producing specific gas products by the catalytic gasification of biomass is presented in this paper. Active catalysts were developed for generation of synthesis gases from wood by steam gasification. A trimetallic catalyst, Ni-Co-Mo on silica-alumina doped with 2 wt% Na, was found to retain activity indefinitely for generation of a methanol synthesis gas from wood at 750° C (1380° F) and 100 kPa (1 atm) absolute pressure. Potassium carbonate was an effective catalyst for conversion of wood to synthesis gases and methane-rich gas and should be economically viable. Results of development studies at 1000 kPa (10 atm) absolute pressure are presented.
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23

Nuñez, Guillermo M., Rosa J. Fenoglio, and Daniel E. Resasco. "Enhanced methane production from methanol decomposition over Pt/TiO2 catalysts." Reaction Kinetics and Catalysis Letters 40, no. 1 (March 1989): 89–94. http://dx.doi.org/10.1007/bf02235144.

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24

Minami, Kiyoshi, Yuichi Tanimoto, Masaharu Tasaki, Shigemichi Ogawa, and Kazuo Okamura. "Influence of pH on methane and sulfide production from methanol." Journal of Fermentation Technology 66, no. 1 (January 1988): 117–21. http://dx.doi.org/10.1016/0385-6380(88)90137-9.

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25

Hoppe, Wieland, Stefan Bringezu, and Nadine Wachter. "Economic assessment of CO2-based methane, methanol and polyoxymethylene production." Journal of CO2 Utilization 27 (October 2018): 170–78. http://dx.doi.org/10.1016/j.jcou.2018.06.019.

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26

Xin, Jia Ying, Jia Liang Jiang, Shuai Zhang, Chao Ze Yan, Ying Xin Zhang, Jing Dong, and Chun Gu Xia. "Use of CAS Colorimetric Assays to Evaluate the Effect of Copper Ion on Methanobactin Production by Methylosinus trichosporium 3011." Advanced Materials Research 549 (July 2012): 50–53. http://dx.doi.org/10.4028/www.scientific.net/amr.549.50.

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Methanobactin (mb) is a small copper-binding chromopeptide produced by methanotrophs. In this paper, a quantitative assay method for the content of mb was developed. The mb produced by Methylosinus trichosporium 3011growth with methane and methanol as carbon sources were detected from the culture supernatants by the CAS colorimetric assay at wavelengths 605 nm. The aim of this study was to evaluate the effect of copper ion on mb production by methane-growth and methanol-growth Methylosinus trichosporium 3011. The results of our experiments prove that Methylosinus trichosporium 3011 is able to utilize methanol as sole source of carbon and energy to produce mb. Cells grown on both methane and methanol exhibited differences in the accumulations of mb which were dependent on the concentration of copper (Ⅱ) present in the growth medium. An increase in the concentration of copper (Ⅱ) in the growth medium decreased mb content in the supernatant solutions. However, the mb was shown to exhibit maximal concentration at 0.5µmol/L copper (Ⅱ) with methanol as carbon source in contrast to the mb from cells grown on methane which as maximum concentration at 0 µmol/L copper (Ⅱ).
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27

Xin, Jia-Ying, Ning Xu, Sheng-Fu Ji, Yan Wang, and Chun-Gu Xia. "Epoxidation of Ethylene by Whole Cell Suspension of Methylosinus trichosporium IMV 3011." Journal of Chemistry 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/9191382.

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Methane monooxygenase (MMO) has been found in methanotrophic bacteria, which catalyzes the epoxidation of gaseous alkenes to their corresponding epoxides. The whole cell suspension of Methylosinus trichosporium IMV 3011 was used to produce epoxyethane from ethylene. The optimal reaction time and initial ethylene concentration for ethylene epoxidation have been described. The product epoxyethane is not further metabolized and accumulates extracellularly. Thus, exhaustion of reductant and the inhibition of toxic products make it difficult to accumulate epoxyethane continuously. In order to settle these problems, regeneration of cofactor NADH was performed in batch experiments with methane and methanol. The amount of epoxyethane formed before cosubstrate regeneration was between 0.8 and 1.0 nmol/50 mg cells in approximately 8 h. Combining data from 7 batch experiments, the total production of epoxyethane was 2.2 nmol. Production of epoxyethane was improved (4.6 nmol) in 10% gas phase methane since methane acts as an abundant reductant for epoxidation. It was found that the maximum production of epoxyethane (6.6 nmol) occurs with 3 mmol/L methanol. The passive effect of epoxyethane accumulation on epoxyethane production capacity of Methylosinus trichosporium IMV 3011 in batch experiments was studied. Removal of product was suggested to overcome the inhibition of epoxyethane production.
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28

Nozhevnikova, Alla N., C. Holliger, A. Ammann, and A. J. B. Zehnder. "Methanogenesis in sediments from deep lakes at different temperatures (2–70°C)." Water Science and Technology 36, no. 6-7 (September 1, 1997): 57–64. http://dx.doi.org/10.2166/wst.1997.0575.

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Methanogenic degradation of organic matter occurs in a wide temperature range from psychrophilic to extreme thermophilic conditions. Mesophilic and thermophilic methanogenesis is relatively well investigated, but little is known about low temperature methanogenesis and psychrophilic methanogenic communities. The aim of the present work was to study methanogenesis in a wide range of temperatures with samples from sediments of deep lakes. These sediments may be considered deposits of different types of microorganisms, which are constantly exposed to low temperatures. The main question was how psychrophilic methanogenic microbial communities compare to mesophilic and thermophilic ones. Methanogenesis in a temperature range of 2–70°C was investigated using sediment samples from Baldegger lake (65 m) and Soppen lake (25 m), Switzerland. Methane production from organic matter of sediments occurred at all temperatures tested. An exponential dependence of methane production rate was found between 2 and 30°C. Methanogenesis occurred even at 70°C. At the same time stable methane production from organic matter of sediments was observed at temperatures below 10°C. Methanogenic microbial communities were enriched at different temperatures. The communities enriched at 4–8°C had the highest activity at low temperatures indicating that a specific psychrophilic community exists. Addition of substrates such as cellulose, volatile fatty acids (butyrate, propionate, acetate), methanol and H2/CO2 stimulated methane production at all temperatures. H2/CO2 as well as methanol were directly converted to methane under thermophilic conditions. At low temperatures these substrates were converted to methane by a two-step process. First acetate was formed, followed by methane production from acetate. When acetate concentrations were high, acetoclastic methanogenesis was inhibited at low temperatures. This reaction appears to be one of the “bottle neck” in psychrophilic methanogenesis.
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Liu, Yong, Matteo Strumendo, and Hamid Arastoopour. "Numerical Simulation of Methane Production from a Methane Hydrate Formation." Industrial & Engineering Chemistry Research 47, no. 8 (April 2008): 2817–28. http://dx.doi.org/10.1021/ie071398b.

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30

Frenzel, Peter, and Ulrike Bosse. "Methyl fluoride, an inhibitor of methane oxidation and methane production." FEMS Microbiology Ecology 21, no. 1 (September 1996): 25–36. http://dx.doi.org/10.1111/j.1574-6941.1996.tb00330.x.

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31

Vallero, M. V. G., G. Lettinga, and P. N. L. Lens. "Assessment of compatible solutes to overcome salinity stress in thermophilic (55¡C) methanol-fed sulfate reducing granular sludges." Water Science and Technology 48, no. 6 (September 1, 2003): 195–202. http://dx.doi.org/10.2166/wst.2003.0396.

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High NaCl concentrations (25 g.L-1) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and methane production. The addition of different osmoprotectants, viz. glutamate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55°C) UASB reactors.
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32

Yoo, Yeon-Sun, Ji-Sun Han, Chang-Min Ahn, Dong-Hee Min, Woo-Jong Mo, Soon-Uk Yoon, Jong-Gyu Lee, Jong-Yeon Lee, and Chang-Gyun Kim. "Characteristics of Methanol Production Derived from Methane Oxidation by Inhibiting Methanol Dehydrogenase." Journal of Korean Society of Environmental Engineers 33, no. 9 (September 30, 2011): 662–69. http://dx.doi.org/10.4491/ksee.2011.33.9.662.

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33

Deines, Peter, and Jonathan Grey. "Site-specific methane production and subsequent midge mediation within Esthwaite Water, UK." Archiv für Hydrobiologie 167, no. 1-4 (October 5, 2006): 317–34. http://dx.doi.org/10.1127/0003-9136/2006/0167-0317.

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34

Akanmu, Abiodun Mayowa, Abubeker Hassen, and Festus Adeyemi Adejoro. "Gas Production, Digestibility and Efficacy of Stored or Fresh Plant Extracts to Reduce Methane Production on Different Substrates." Animals 10, no. 1 (January 16, 2020): 146. http://dx.doi.org/10.3390/ani10010146.

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Natural compounds such as plant secondary metabolites (PSM) can be used to replace antibiotic growth promoters as rumen modifiers. In this study, the effectiveness of stored and freshly extracted Aloe vera (AV), Azadirachta indica (AZ), Moringa oleifera (MO), Jatropha curcas (JA), Tithonia diversifolia (TD) and Carica papaya (CP) crude extract and monensin on in vitro gas and methane production, organic matter digestibility (IVOMD) and volatile fatty acids (VFA) were evaluated using a total mixed ration (TMR), lucerne or Eragrostis curvula substrates. Fresh extracts were processed from the same batch of frozen (−20 °C) plant material a few days before the trial while the stored extracts were extracted and stored at 4 °C for 12 months prior to the study. Extraction was done by solubilising 50 g freeze-dried plant material in 500 mL 100% methanol. Four mL of reconstituted 50 mg crude extract per 1000 mL distilled water was added per incubation vial, which already contained 400 mg substrate and in vitro fermentation, and gas production and IVOMD evaluation were carried out using standard procedures. Results showed that storing plant extracts for 12 months did not affect the activity or stability of metabolites present in the crude extracts, as shown by the lack of differences in total gas production (TGP) and methane produced between fresh or stored extracts across the substrates. In the TMR substrate, plant extracts increased IVOMD but did not affect TGP and methane production, whereas monensin did not have any effect. Plant extracts increased IVOMD of Eragrostis substrate and supressed methane production to a greater extent than monensin (p < 0.05). It can be concluded that storing plant extracts for up to 12 months did not compromise their efficacy. In addition, the use of 50 mg/kg of AV, AZ, MO, JA, TD and CP extract to a forage-based diet will reduce methane production while improving feed digestibility.
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35

Dong, Jing, Jia Ying Xin, Ying Xin Zhang, Lin Lin Chen, Hong Ye Liang, and Chun Gu Xia. "Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis." Advanced Materials Research 160-162 (November 2010): 171–75. http://dx.doi.org/10.4028/www.scientific.net/amr.160-162.171.

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Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.
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36

Zaccara, Antonella, Alice Petrucciani, Ismael Matino, Teresa Annunziata Branca, Stefano Dettori, Vincenzo Iannino, Valentina Colla, Michael Bampaou, and Kyriakos Panopoulos. "Renewable Hydrogen Production Processes for the Off-Gas Valorization in Integrated Steelworks through Hydrogen Intensified Methane and Methanol Syntheses." Metals 10, no. 11 (November 18, 2020): 1535. http://dx.doi.org/10.3390/met10111535.

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Within integrated steelmaking industries significant research efforts are devoted to the efficient use of resources and the reduction of CO2 emissions. Integrated steelworks consume a considerable quantity of raw materials and produce a high amount of by-products, such as off-gases, currently used for the internal production of heat, steam or electricity. These off-gases can be further valorized as feedstock for methane and methanol syntheses, but their hydrogen content is often inadequate to reach high conversions in synthesis processes. The addition of hydrogen is fundamental and a suitable hydrogen production process must be selected to obtain advantages in process economy and sustainability. This paper presents a comparative analysis of different hydrogen production processes from renewable energy, namely polymer electrolyte membrane electrolysis, solid oxide electrolyze cell electrolysis, and biomass gasification. Aspen Plus® V11-based models were developed, and simulations were conducted for sensitivity analyses to acquire useful information related to the process behavior. Advantages and disadvantages for each considered process were highlighted. In addition, the integration of the analyzed hydrogen production methods with methane and methanol syntheses is analyzed through further Aspen Plus®-based simulations. The pros and cons of the different hydrogen production options coupled with methane and methanol syntheses included in steelmaking industries are analyzed.
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37

Moss, Angela R., and D. I. Givens. "Methane production from weaned dairy heifer calves." Proceedings of the British Society of Animal Science 1998 (1998): 54. http://dx.doi.org/10.1017/s0308229600032670.

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Agriculture is one of the major sources of methane in the UK and the major contribution is that from the ruminant animal. Most current inventories include emissions from growing and adult cattle and it has been assumed that the young calf contributes little to the methane flux. There is a dearth of information for young cattle (65-110 kg liveweight) and the objective here was to provide methane data for this group of ruminants to assist in improving the UK inventories for methane.
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38

Chen, Luning, Zhiyuan Qi, Shuchen Zhang, Ji Su, and Gabor A. Somorjai. "Catalytic Hydrogen Production from Methane: A Review on Recent Progress and Prospect." Catalysts 10, no. 8 (August 2, 2020): 858. http://dx.doi.org/10.3390/catal10080858.

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Natural gas (Methane) is currently the primary source of catalytic hydrogen production, accounting for three quarters of the annual global dedicated hydrogen production (about 70 M tons). Steam–methane reforming (SMR) is the currently used industrial process for hydrogen production. However, the SMR process suffers with insufficient catalytic activity, low long-term stability, and excessive energy input, mostly due to the handling of large amount of CO2 coproduced. With the demand for anticipated hydrogen production to reach 122.5 M tons in 2024, novel and upgraded catalytic processes are desired for more effective utilization of precious natural resources. In this review, we summarized the major descriptors of catalyst and reaction engineering of the SMR process and compared the SMR process with its derivative technologies, such as dry reforming with CO2 (DRM), partial oxidation with O2, autothermal reforming with H2O and O2. Finally, we discussed the new progresses of methane conversion: direct decomposition to hydrogen and solid carbon and selective oxidation in mild conditions to hydrogen containing liquid organics (i.e., methanol, formic acid, and acetic acid), which serve as alternative hydrogen carriers. We hope this review will help to achieve a whole picture of catalytic hydrogen production from methane.
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39

Haryati, Tuti, A. P. Sinurat, B. Listian, H. Hamid, and T. Purwadaria. "Application of BS4-enzyme on the methane production from mixture of cattle manures and waste paper." Jurnal Ilmu Ternak dan Veteriner 21, no. 4 (January 11, 2018): 238. http://dx.doi.org/10.14334/jitv.v21i4.1524.

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<p class="abstrak2">Cellulose from abundant newspaper waste could be transformed into methane through anaerobic fermentation. This research was carried out to compare the gas production including methane between samples containing feces and waste paper mixture as inoculum and substrate, respectively and added with and without BS4 enzyme. The enzyme was produced in Indonesian Research Institute of Animal Produce (IRIAP) by growing Eupenicillium javanicum BS4 in coconut meals. There were three treatments, i.e., 30% manure (M30); 15 % manure + 15 % paper waste (MP 30); MP30 + 3 mL BS4 enzyme equal to 0.42 U/g dry matter (MPE30) The percentage of waste papers addition in feces was calculated on dry matter (DM) basis and every treatment had five replications. Total gas and methane productions were measured weekly, while dry matter losses were determined during 5 week fermentation. Interactions between treatments and incubation time were analyzed using completely randomized design each week. Kind of substrates influenced both total gas and methane productions during incubation time. Both waste papers and enzyme addition enhanced gas production. The highest total gas and methane productions for five weeks incubation were highly significantly observed (P&lt;0.01) in MP30 and MPE30 compared to M30. Addition of enzyme significantly increased total gas and methane productions in the first week. The highest methane and total gas yield/g dry matter were obtained by BS4 enzyme addition. It was concluded that BS4 enzyme is good in accelerating and increasing the transformation efficiency of waste paper and manure mixture for biogas production.</p><p><strong>Key Words</strong>: Methane, Fibrenolytic-Enzyme, Waste Papers, Cattle Manures</p>
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40

Thu, Nguyễn Thị Hiếu, and Đinh Thuý Hằng. "Isolation of a methane-oxydizing bacterium for the study on single cell protein production from methane." Vietnam Journal of Biotechnology 14, no. 3 (September 30, 2016): 581–88. http://dx.doi.org/10.15625/1811-4989/14/3/9876.

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Single cell protein (SCP) can be produced from biomass of different types of microorganisms that have high protein content such as yeast, filamentous fungi, algae and bacteria. In comparison to animal and plant protein sources, this kind of protein has several advantages, namely high protein and nutrient contents, being produced in fermenters with the use of variety of organic wastes, independence in agriculture land or climate conditions. Methane oxidizing bacteria (MOB) are considered as good candidates for SCP production and have been intensively studied recently. In the present study, a MOB strain BG3 was isolated from wastewater of an anaerobic digester via enrichment and isolation procedures using methane as the only carbon and energy sources. Strain BG3 comprised of oval-shaped cells of 0,8-1´16 -1,8 μm in size, almost nonmotile. Based on comparative analyses of the 16S rDNA partial sequences, strain BG3 was identified as a member of the Methylomonas genus, the most closely related species was Methylomonas koyamae (97% homology). This was also confirmed by analyses of sequence of the pmoA gene, encoding for a-subunit in the methane-monooxygenase in the strain. Besides methane, strain BG3 also utilized methanol for the growth. It has been shown that methane-fed culture of strain BG3 could produce 68.69 g crude protein per 100 g CDW and the according methane to biomass conversion efficiency was 2,8 m3 methane×kg-1 dry biomassas. Owing the capability of utilization of methane, the second important greenhouse gas, for the production of protein source for animal feed, strain BG3 would have a great application potential in Vietnam. Strain BG3 was designated as Methylomonas sp. BG3 and its 16S rDNA and pmoA gene sequences were deposited at the GenBank with accession numbers of KJ081955 and KJ081956, respectively.
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41

Prabhudessai, Vidhya, Bhakti Salgaonkar, Judith Braganca, and Srikanth Mutnuri. "Pretreatment of Cottage Cheese to Enhance Biogas Production." BioMed Research International 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/374562.

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This study evaluated the possibility of pretreating selected solid fraction of an anaerobic digester treating food waste to lower the hydraulic retention time and increase the methane production. The study investigated the effect of different pretreatments (thermal, chemical, thermochemical and enzymatic) for enhanced methane production from cottage cheese. The most effective pretreatments were thermal and enzymatic. Highest solubilisation of COD was observed in thermal pretreatment, followed by thermochemical. In single enzyme systems, lipase at low concentration gave significantly higher methane yield than for the experiments without enzyme additions. The highest lipase dosages decreased methane yield from cottage cheese. However, in case of protease enzyme an increase in concentration of the enzyme showed higher methane yield. In the case of mixed enzyme systems, pretreatment at 1 : 2 ratio of lipase : protease showed higher methane production in comparison with 1 : 1 and 2 : 1 ratios. Methane production potentials for different pretreatments were as follows: thermal 357 mL/g VS, chemical 293 mL/g VS, and thermochemical 441 mL/g VS. The average methane yield from single enzyme systems was 335 mL/g VS for lipase and 328 mL/g VS for protease. Methane potentials for mixed enzyme ratios were 330, 360, and 339 mL/g VS for 1 : 1, 1 : 2, and 2 : 1 lipase : protease, respectively.
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42

Catlett, Jennie L., Alicia M. Ortiz, and Nicole R. Buan. "Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production." Applied and Environmental Microbiology 81, no. 19 (July 10, 2015): 6528–37. http://dx.doi.org/10.1128/aem.01162-15.

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ABSTRACTMethanogens are anaerobic archaea that grow by producing methane, a gas that is both an efficient renewable fuel and a potent greenhouse gas. We observed that overexpression of the cytoplasmic heterodisulfide reductase enzyme HdrABC increased the rate of methane production from methanol by 30% without affecting the growth rate relative to the parent strain. Hdr enzymes are essential in all known methane-producing archaea. They function as the terminal oxidases in the methanogen electron transport system by reducing the coenzyme M (2-mercaptoethane sulfonate) and coenzyme B (7-mercaptoheptanoylthreonine sulfonate) heterodisulfide, CoM-S-S-CoB, to regenerate the thiol-coenzymes for reuse. InMethanosarcina acetivorans, HdrABC expression caused an increased rate of methanogenesis and a decrease in metabolic efficiency on methylotrophic substrates. When acetate was the sole carbon and energy source, neither deletion nor overexpression of HdrABC had an effect on growth or methane production rates. These results suggest that in cells grown on methylated substrates, the cell compensates for energy losses due to expression of HdrABC with an increased rate of substrate turnover and that HdrABC lacks the appropriate electron donor in acetate-grown cells.
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43

NAGAO, Jiro. "Development of methane hydrate production method." Synthesiology English edition 5, no. 2 (2012): 88–95. http://dx.doi.org/10.5571/syntheng.5.88.

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44

McCaughey, W. P., K. Wittenberg, and D. Corrigan. "Methane production by steers on pasture." Canadian Journal of Animal Science 77, no. 3 (September 1, 1997): 519–24. http://dx.doi.org/10.4141/a96-137.

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In order to determine the quantity of methane (CH4) produced by steers on pasture, 16 steers with a mean weight of 356 ± 25 kg were randomly selected from a larger group of cattle (n = 48) to evaluate the effects of grazing management and monensin controlled release capsule (CRC) administration on ruminal CH4 production using the sulphur hexafluoride (SF6) tracer-gas technique. Pasture management treatments consisted of two grazing systems (continuous stocking or 10-paddock rotational stocking) at each of two stocking rates (low, 1.1 steer ha−1 or high, 2.2 steers ha−1) with two replications of each pasture treatment. Half of the animals on each pasture treatment were administered a monensin CRC delivering 270 mg d−1, and untreated animals served as controls. During the 140-d grazing season, one steer from each treatment-replicate combination was sampled to determine daily intake and CH4 production on four occasions. The chemical composition of diets differed between grazing management treatments and sampling periods. Voluntary intake and CH4 production, adjusted for differences in body weight, were unaffected by grazing management, sampling period or by monensin CRC administration and averaged 0.69 ± 0.1 L kg BW−1 d−1 across all grazing management treatments. The energy lost through eructation of CH4 averaged 4.5 ± 1.4% of gross energy intake. Key words: Methane, cattle, environment, digestion efficiency, pasture, forage
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45

Bhatta, Raghavendra, and Osamu Enishi. "Measurement of Methane Production from Ruminants." Asian-Australasian Journal of Animal Sciences 20, no. 8 (June 27, 2007): 1305–18. http://dx.doi.org/10.5713/ajas.2007.1305.

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46

NAGAO, Jiro. "Development of methane hydrate production method." Synthesiology 5, no. 2 (2012): 89–97. http://dx.doi.org/10.5571/synth.5.89.

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47

Watson, Andrea, and David B. Nedwell. "Methane production and emission from peat." Atmospheric Environment 32, no. 19 (October 1998): 3239–45. http://dx.doi.org/10.1016/s1352-2310(97)00501-3.

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48

Kobayashi, Fumihisa, Harumi Take, Chikako Asada, and Yoshitoshi Nakamura. "Methane production from steam-exploded bamboo." Journal of Bioscience and Bioengineering 97, no. 6 (January 2004): 426–28. http://dx.doi.org/10.1016/s1389-1723(04)70231-5.

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49

Hao, Wei Min, and Darold E. Ward. "Methane production from global biomass burning." Journal of Geophysical Research 98, no. D11 (1993): 20657. http://dx.doi.org/10.1029/93jd01908.

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50

SHIBATA, Masaki, Fuminori TERADA, Mitsunori KURIHARA, Takehiro NISHIDA, and Kazuo IWASAKI. "Estimation of Methane Production in Ruminants." Nihon Chikusan Gakkaiho 64, no. 8 (1993): 790–96. http://dx.doi.org/10.2508/chikusan.64.790.

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