Relevant bibliographies by topics / Promoter Characterization

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Promoter Characterization'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 September 2023

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Journal articles on the topic "Promoter Characterization"

1

Nevoigt, Elke, Jessica Kohnke, Curt R. Fischer, Hal Alper, Ulf Stahl, and Gregory Stephanopoulos. "Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae." Applied and Environmental Microbiology 72, no. 8 (2006): 5266–73. http://dx.doi.org/10.1128/aem.00530-06.

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ABSTRACT The strong overexpression or complete deletion of a gene gives only limited information about its control over a certain phenotype or pathway. Gene function studies based on these methods are therefore incomplete. To effect facile manipulation of gene expression across a full continuum of possible expression levels, we recently created a library of mutant promoters. Here, we provide the detailed characterization of our yeast promoter collection comprising 11 mutants of the strong constitutive Saccharomyces cerevisiae TEF1 promoter. The activities of the mutant promoters range between
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Zhang, Xiaojuan, Zhixuan Yao, Yanting Duan, Xiaomei Zhang, Jinsong Shi, and Zhenghong Xu. "Investigation of specific interactions between T7 promoter and T7 RNA polymerase by force spectroscopy using atomic force microscope." Biochemical Journal 475, no. 1 (2018): 319–28. http://dx.doi.org/10.1042/bcj20170616.

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The specific recognition and binding of promoter and RNA polymerase is the first step of transcription initiation in bacteria and largely determines transcription activity. Therefore, direct analysis of the interaction between promoter and RNA polymerase in vitro may be a new strategy for promoter characterization, to avoid interference due to the cell's biophysical condition and other regulatory elements. In the present study, the specific interaction between T7 promoter and T7 RNA polymerase was studied as a model system using force spectroscopy based on atomic force microscope (AFM). The sp
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Daxhelet, Guy, Philippe Gilot, and Philippe Hoet. "Cloning and characterization of transcriptional promoters fromBacillus subtilisphage 2C." Canadian Journal of Microbiology 42, no. 9 (1996): 919–26. http://dx.doi.org/10.1139/m96-118.

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Phage 2C is a Bacillus subtilis lytic phage, whose genome contains hydroxymethyluracil in place of thymine. To isolate promoters of early phage genes involved in the take-over of cellular metabolism, 2C DNA libraries were constructed in promoter-probe plasmids replicating in Escherichia coli and B. subtilis. Four different 2C DNA fragments strongly expressed reporter genes in E. coli but not in B. subtilis. All fragments originated from unique sequences of the genome and not from its terminal redundancies. One fragment was sequenced. Despite the presence of an σA-RNA polymerase binding site up
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Gopaul, Krishna K., Patricia C. Brooks, Jean-François Prost, and Elaine O. Davis. "Characterization of the Two Mycobacterium tuberculosis recA Promoters." Journal of Bacteriology 185, no. 20 (2003): 6005–15. http://dx.doi.org/10.1128/jb.185.20.6005-6015.2003.

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ABSTRACT The recA gene of Mycobacterium tuberculosis is unusual in that it is expressed from two promoters, one of which, P1, is DNA damage inducible independently of LexA and RecA, while the other, P2, is regulated by LexA in the classical way (E. O. Davis, B. Springer, K. K. Gopaul, K. G. Papavinasasundaram, P. Sander, and E. C. Böttger, Mol. Microbiol. 46:791-800, 2002). In this study we characterized these two promoters in more detail. Firstly, we localized the promoter elements for each of the promoters, and in so doing we identified a mutation in each promoter which eliminates promoter
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Zahradka, Peter, Dawn E. Larson, and Bruce H. Sells. "Characterization of a mammalian ribosomal protein gene promoter." Biochemistry and Cell Biology 68, no. 6 (1990): 949–56. http://dx.doi.org/10.1139/o90-140.

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The presence of specific promoter elements, notably the TATA and GC boxes, has been useful for categorizing genes transcribed by RNA polymerase II. The gene for the murine ribosomal protein (r-protein) L32 lacks both of these elements, although it has GC-rich regions. The conditions required for its optimal synthesis in vitro, however, resemble the properties of promoters containing TATA (adenovirus major late promoter) rather than GC boxes (dihydrofolate reductase). To further investigate the relationship of the r-protein gene to different promoter elements, transcription competition analyses
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Gosselin, P., A. P. Makrigiannis, R. Nalewaik, and S. K. Anderson. "Characterization of the Ly49I promoter." Immunogenetics 51, no. 4-5 (2000): 326–31. http://dx.doi.org/10.1007/s002510050626.

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Dutt, Manjul, Zhijian T. Li, Sadanand Dhekney, and Dennis J. Gray. "(285) Characterization of a Composite Promoter from Genomic Sequences of Grapevine." HortScience 41, no. 4 (2006): 1053C—1053. http://dx.doi.org/10.21273/hortsci.41.4.1053c.

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Genetic transformation of plants necessitates the use of promoters to control transgene expression. Numerous promoters have been isolated from a wide range of organisms for use in plants. However, many of these natural promoters exhibit relatively low activity and/or have limited use. To provide an alternative, we constructed a composite promoter (EP) using a genomic DNA sequence and a 35 bp TATA-containing fragment from the 2S albumin (VvAlb1) gene core promoter of grapevine. The 0.9-kb genomic sequence was identified after TAIL-PCR, based on the presence of several unique cis-acting elements
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Cazzonelli, Christopher Ian, and Jeff Velten. "In vivo characterization of plant promoter element interaction using synthetic promoters." Transgenic Research 17, no. 3 (2007): 437–57. http://dx.doi.org/10.1007/s11248-007-9117-8.

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Bacha, Sardar, Nadia Iqbal, Aftab Bashir, Farah Deeba, Muhammad Asif, and Joshua Yuan. "Evaluation of Cis-Regulatory Elements of Dicot ?-TbSt Promoter." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 2, no. 3 (2021): 23–38. http://dx.doi.org/10.52700/jmmg.v2i3.35.

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The eukaryotic gene expression is controlled by a regulatory region called promoter. Many plant promoters have been characterized for regulatory motifs. There are three types of plant promoters i.e. inducible, constitutive and tissue specific on basis of regulatory motifs. Plant sources have been searched for isolation of strong promoters that are being utilized in molecular biology research. The researchers need to address IPR issues for utilizing the strong patented promoters for the expression of their transgenes. The identification and characterization of strong dicot promoter is necessary
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García, Pilar, Juan Evaristo Suárez, Victoria Bascarán, and Ana Rodríguez. "Isolation and characterization of promoters from the Lactobacillus casei temperate bacteriophage A2." Canadian Journal of Microbiology 43, no. 11 (1997): 1063–68. http://dx.doi.org/10.1139/m97-151.

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Random Sau3AI DNA fragments from the temperate Lactobacillus bacteriophage A2 were cloned into the promoter-probe plasmid pGKV210. Seven DNA fragments with promoter activity were selected, after transformation of Escherichia coli and Lactococcus lactis, subsp. lactis, through the chloramphenicol resistance they conferred to the corresponding clones. The seven promoters were functional in Lactobacillus casei. Their strength was analysed by measuring the levels of chloramphenicol resistance and chloramphenicol acetyltransferase activity induced in each host. The nucleotide sequences of these fra
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Dissertations / Theses on the topic "Promoter Characterization"

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Louis, Vanessa. "Human promoter characterization." DigitalCommons@Robert W. Woodruff Library, Atlanta University Center, 2010. http://digitalcommons.auctr.edu/dissertations/153.

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The basic-helix-loop-helix (bHLH) protein family plays an innate role in cellular activities. A pivotal member of this family, the Inhibitor of differentiation (Id) proteins, is linked to cell proliferation, differentiation, and tumorigenesis via inhibiting the DNA binding of bHLH proteins. There are four isoforms of Id genes - Id1-4 are uniquely expressed. In prostate cancer cells, Id3 is up-regulated and targets cellular proliferation and metastasis. However, the mechanism that triggers Id3 expression in prostate cancer is unknown. Therefore, a series of experiments were performed in order t
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Kilcup, Michael. "Characterization of the murine Unp promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32539.pdf.

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Ezpeleta, Jessica. "The characterization of the ABF-1 promoter." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/559.

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The basic helix-loop-helix (bHLH) family oftranscription factors consists of proteins involved in cellular proliferation and differentiation. The HLH structure plays a key role in protein-protein dimerization and with the DNA target sites, referred to as E boxes containing the consensus DNA sequence CANNTG. One class of mammalian class I bHLH proteins includes products of the E2A gene, which result from alternative splicing (E12, E47, and ITF), E2-2 and HEB. E2A proteins have also been detected in most cell lines with high levels of expression in lymphoid- and pancreatic cells. It has also bee
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Bodner, Caroline. "Identification and characterization of the murine Meis1 promoter." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=83966.

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MEIS1 (myeloid ecotropic viral integration site 1) is a member of the TALE class of homeodomain-containing proteins. MEIS1, along with related family members PBX and PREP1, bind DNA cooperatively with HOX proteins to regulate transcription. Meis1 is implicated in many developmental processes. Moreover, deregulation of Meis1 is involved in leukemogenesis. To better understand the mechanisms of Meis1 transcriptional regulation, we set out to map and characterize the Meis1 promoter. Results from RNase protection and 5' RACE suggest that Meis1 has multiple transcription start sites (TSSs) l
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Morris, Terry Lynn. "Molecular characterization of the fepA-fes bidirectional promoter in escherichia coli." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3025640.

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Jacobs, Sarah Margaret. "CHARACTERIZATION OF A PROMOTER REARRANGEMENT AND A SECOND PROMOTER IN THE HUMAN C-MYB PROTO-ONCOGENE." VCU Scholars Compass, 1993. http://scholarscompass.vcu.edu/etd/4938.

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The human cellular proto-oncogene c-myb has been implicated as important in the regulation of hematopoietic cell growth and differentiation. Aberrant expression of this gene and chromosomal aberrations near the c-myb locus have been associated with a number of carcinogenic processes. An alternatively spliced CDNA clone of c-myb, pMbm-2, contains unique 5’ sequences which replace exon 1. The existence of this 5’ divergent CDNA clone led us into a study of the promoter activity of the c-myb gene. Intron 1 of c-myb is highly conserved between human and mouse throughout the intron, while only thos
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Blundell, Matthew Charles. "Characterization of the promoter in a human B19 parvovirus." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/29011.

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The nucleotide sequence of the B19-Wi isolate of an autonomous human parvovirus was determined and compared with the sequence of the closely related isolate, B19-Au. The B19-Wi genome was similar to the B19-Au genome, as shown from DNA sequence analyses. It had been previously suggested from the sequence of the B19-Au genome, that the termini may be imperfect inverted terminal repeats. The additional sequence present on the right-hand terminus of B19-Wi supported that supposition. The hairpin termini of the B19 genome were of the same type as those found in adeno-associated parvoviruses, and s
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Chen, Zhiqi. "Cloning and characterization of the human CD200 promoter region." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ63146.pdf.

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Altmann, Stephen. "Molecular Characterization of Zebrafish Interferon, MX, and MX Promoter." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/AltmannSM2003.pdf.

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Johnson, Sabrina D. "Characterization of the Pichia pastoris alcohol oxidase I promoter." Scholarly Commons, 2003. https://scholarlycommons.pacific.edu/uop_etds/575.

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The methylotrophic yeast, Pichia past oris, is one of the most respected and widely used systems today. The ability of this yeast to produce large masses of protein and metabolize methanol as a sole source of carbon and energy is attributed to the highly induceable Alcohol Oxidase I promoter (AOXI). Despite of the disperse popularity and use of this promoter over the last 15 years, little is known about the transcription controls at a molecular level. A 5'>3' deletion analysis of the AOXI promoter was perrormed to gain understanding of the promoter's regulation and provided insight to the appr
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Books on the topic "Promoter Characterization"

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Matheson, Kimberly. Functional characterization of the RHD promoter. National Library of Canada, 2002.

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Spek, C. Arnold. Characterization of the human protein C gene promoter. University of Leiden, 1998.

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Chen, Zhiqi. Cloning and characterization of the human CD200 promoter region. National Library of Canada, 2001.

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Kim, Soo-yeon Sammy. Characterization of a BMP responsive element in the Tlx2 promoter. National Library of Canada, 1999.

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Li, Jack Jun. Cloning and characterization of the rat bone sialoprotein (BSP) gene promoter. University of Toronto, Faculty of Dentistry], 1998.

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Karaskov, Elizabeta. In vivo characterization of ordered factor recruitment at CIITA inducible promoter IV. National Library of Canada, 2003.

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Shufflebottom, Diane. Characterization of the promoter activities of two different phenylalanine ammonia-lyase genes. University of East Anglia, 1992.

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Kim, Richard Hyung-Jun. Characterization of the human bone-sialoprotein BSP gene and its promoter sequence. National Library of Canada, 1993.

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Gill, Robert James Montgomery. Characterization of the human RB1 promoter and of elements involved in transcriptional autoregulation. National Library of Canada = Bibliothèque nationale du Canada, 1993.

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Paramaswara, Bamini. Characterization of the promoter for the human factor I (C3b/C4b inactivator) gene. National Library of Canada, 1998.

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Book chapters on the topic "Promoter Characterization"

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Chen, Hsiao-Huei, and Alexandre F. R. Stewart. "Characterization of Cardiac Gene Promoter Activity." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-030-0_12.

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Chung-Bok, M. I., and M. Watford. "Characterization of the Hepatic Glutaminase Promoter." In Contributions to Nephrology. KARGER, 1997. http://dx.doi.org/10.1159/000059843.

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Iland, Harry J., Jacqui Thorn, and Peter Molloy. "Characterization of N-RAS Promoter Mutations In Leukemia." In Molecular Biology of Hematopoiesis 5. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0391-6_26.

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Guo, Ailong, Ping Qin, Weiwei Shi, Yuyin Li, and Aipo Diao. "Cloning and Characterization of the TMEPAI Gene Promoter." In Lecture Notes in Electrical Engineering. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46318-5_7.

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Zarain-Herzberg, Angel, and Georgina Alvarez-Fernández. "The SERCA2 gene: genomic organization and promoter characterization." In Signal Transduction and Cardiac Hypertrophy. Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0347-7_35.

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Thorn, J. T., A. V. Todd, D. Warrilow, F. Watt, P. L. Molloy, and H. J. Iland. "Characterization of the Human N-ras Promoter Region." In The Superfamily of ras-Related Genes. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-6018-6_11.

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Echeverria, Manuel, Dominique Tremousaygue, Thomas Roscoe, Katia Wassmann, Paul Penon, and Michel Delseny. "Characterization of Protein Factors Interacting with the rDNA Promoter Region." In Plant Molecular Biology. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78852-9_38.

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Li, Ian M. H., Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, and George Bou-Gharios. "Characterization of Mesenchymal-Fibroblast Cells Using the Col1a2 Promoter/Enhancer." In Fibrosis. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7113-8_10.

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Teixeira, K. R. S., T. Morgan, D. Meletzus, R. Galler, J. I. Baldani, and C. Kennedy. "Acetobacter diazotrophicus: nifA-Like Gene and Characterization of a nif-Like Promoter." In Biological Nitrogen Fixation for the 21st Century. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_69.

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Han, Xiaobin, Andrea M. Budreau, and Russell W. Chesney. "Cloning and Characterization of the Promoter Region of the Rat Taurine Transporter (TauT) Gene." In Advances in Experimental Medicine and Biology. Springer US, 2002. http://dx.doi.org/10.1007/0-306-46838-7_9.

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Conference papers on the topic "Promoter Characterization"

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Lin, Sheng-Yi, Yi-Hua Lai, Hsuan-Yu Chen, et al. "Abstract 749: Characterization of p54nrb/NonO promoter in NSCLC." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-749.

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Abelniece, Zane, Helle-Mai Piirsoo, Hugo Mandar, and Aile Tamm. "CHARACTERIZATION AND ACTIVITY OF COBALT BASED SBA-15 SUPPORTED CATALYSTS FOR CARBON DIOXIDE HYDROGENATION." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/4.1/s17.02.

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Utilization of renewable energy sources is future solution for providing the word energy demand and to solve the problems of climate changes. Carbon dioxide is greenhouse gas and major contributor to the global warming. Hydrogenation process of carbon dioxide with renewable hydrogen will not only mitigate the problem of global warming but will also produce valuable products and renewable energy. The hydrogenation of carbon dioxide to value-added chemicals and fuels is a promising route, providing products such as carbon monoxide, methanol, hydrocarbons, and higher alcohols. Among these, the sy
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Rohinikumar, P., M. G. Muni Reddy, P. Venkateswarlu, and K. V. Ramesh. "Gas holdup in a bubble column in the presence of coaxially placed string of spheres promoter as internal." In INTERNATIONAL CONFERENCE ON FUNCTIONAL MATERIALS, CHARACTERIZATION, SOLID STATE PHYSICS, POWER, THERMAL AND COMBUSTION ENERGY: FCSPTC-2017. Author(s), 2017. http://dx.doi.org/10.1063/1.4990229.

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Yang, Qian, Yuzhen Lin, Quanhong Xu, Chi Zhang, and Chih-Jen Sung. "Cooling Effectiveness of Impingement/Effusion Cooling With and Without Turbulence Promoter Ribs." In ASME Turbo Expo 2012: Turbine Technical Conference and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/gt2012-69209.

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In order to solve the cooling problem of aeroengine, a variety of highly efficient cooling methods came into application, such as effusion wall, impingement/effusion wall, etc. The impingement/effusion cooling methods with and without turbulence promoter ribs are introduced here. In the impingement/promoter ribs/effusion walls, turbulence promoter ribs are on the coolant side of effusion wall so as to enhance the internal cooling. Numerical and experimental approaches were taken to study cooling effectiveness of impingement/effusion liners cooling with and without turbulence promoter ribs. Coo
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Brown, Robert V., Vanessa C. Gaerig, Jessica S. Fortin, and Tracy A. Brooks. "Abstract 3681: Helping Eve overcome ADAM: Characterization of the G-quadruplex in the ADAM15 core promoter." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-3681.

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Chen, Kun, Yong Jiang, Nan Wang, Xuegang Luo, Fuping Lu, and Tongcun Zhang. "Cloning, Characterization and Application of the Promoter Region of the Alkaline Protease Gene in Bacillus alcalophillus PB92." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162515.

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Ota, Takayo, Amy C. Clayton, Viji Shridhar, Ichiro Miyoshi, and Jeremy Chien. "Abstract 3276: Characterization of tumors in mouse oviduct-specific glycoprotein 1(Ogp1) promoter-driven SV40 large T antigen." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-3276.

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"Experimental in vitro characterization of TATA-binding protein to TATA-boxes affinity of Arabidopsis thaliana real promoter genes." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-363.

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Basyuni, Mohammad, Ridha Wati, Shigeyuki Baba, and Hirosuke Oku. "Characterization of cycloartenol synthase KcCAS promoter region from mangrove plant (Kandelia obovata Sheue, H. Y. Liu & J. Yong)." In INVENTING PROSPEROUS FUTURE THROUGH BIOLOGICAL RESEARCH AND TROPICAL BIODIVERSITY MANAGEMENT: Proceedings of the 5th International Conference on Biological Science. Author(s), 2018. http://dx.doi.org/10.1063/1.5050136.

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Ota, Takayo, Ronald J. Marler, Amy C. Clayton, et al. "Abstract 3272: Characterization of mouse oviductal glycoprotein (Ovgp1) promoter driven SV40 T large antigen: fallopian tube cancer and leiomyosarcoma mouse model." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3272.

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Reports on the topic "Promoter Characterization"

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Raviv, Michael, and K. W. Mudge. Chemical Identification, Physiological Characterization and Horticultural Applications of a Rooting Promoter Extracted from Avocado Tissues. United States Department of Agriculture, 1990. http://dx.doi.org/10.32747/1990.7695831.bard.

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Dougherty, Shona. Isolation and Functional Characterization of Prostate Tumor-Specific Hypoxia-Inducible Promoter/Enhancer Elements for Use in Gene Therapy. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada413596.

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Shapira, Roni, Judith Grizzle, Nachman Paster, Mark Pines, and Chamindrani Mendis-Handagama. Novel Approach to Mycotoxin Detoxification in Farm Animals Using Probiotics Added to Feed Stuffs. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592115.bard.

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T-2 toxin, a toxic product belongs to the trichothecene mycotoxins, attracts major interest because of its severe detrimental effects on the health of human and farm animals. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The hypothesis of this project was to develop a protecting system using probiotic bacteria that will express trichothecene 3-O-acetyltransferase (Tri101) that convert T-2 to a less toxic intermediate to reduce inges
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Zhou, Ting, Roni Shapira, Peter Pauls, Nachman Paster, and Mark Pines. Biological Detoxification of the Mycotoxin Deoxynivalenol (DON) to Improve Safety of Animal Feed and Food. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7613885.bard.

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The trichothecene deoxynivalenol (DON, vomitoxin), one of the most common mycotoxin contaminants of grains, is produced by members of the Fusarium genus. DON poses a health risk to consumers and impairs livestock performance because it causes feed refusal, nausea, vomiting, diarrhea, hemolytic effects and cellular injury. The occurrence of trichothecenes contamination is global and they are very resistant to physical or chemical detoxification techniques. Trichothecenes are absorbed in the small intestine into the blood stream. The overall objective of this project was to develop a protecting
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Lers, Amnon, and Gan Susheng. Study of the regulatory mechanism involved in dark-induced Postharvest leaf senescence. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7591734.bard.

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Postharvest leaf senescence contributes to quality losses in flowers and leafy vegetables. The general goal of this research project was to investigate the regulatory mechanisms involved in dark-induced leaf senescence. The regulatory system involved in senescence induction and control is highly complex and possibly involves a network of senescence promoting pathways responsible for activation of the senescence-associated genes. Pathways involving different internal signals and environmental factors may have distinctive importance in different leaf senescence systems. Darkness is known to have
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Xu, Jin-Rong, and Amir Sharon. Comparative studies of fungal pathogeneses in two hemibiotrophs: Magnaporthe grisea and Colletotrichum gloeosporioides. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7695585.bard.

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Plant pathogenic fungi have various life styles and different plant infection strategies. Hemibiotrophs like Magnaporthe grisea and Colletotrichum species develop specialized structures during plant infection. The goal of this study was to identify, characterize, and compare genes required for plant infection in M. grisea and C. gloeosporioides. Specific objectives are to: 1) further characterize genes identified in the preliminary studies of C. gloeosporioides and M. grisea;2) identify and characterize additional fungal genes tagged by GFP; and 3) identify in planta growth and appressorium-sp
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Epel, Bernard L., Roger N. Beachy, A. Katz, et al. Isolation and Characterization of Plasmodesmata Components by Association with Tobacco Mosaic Virus Movement Proteins Fused with the Green Fluorescent Protein from Aequorea victoria. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7573996.bard.

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The coordination and regulation of growth and development in multicellular organisms is dependent, in part, on the controlled short and long-distance transport of signaling molecule: In plants, symplastic communication is provided by trans-wall co-axial membranous tunnels termed plasmodesmata (Pd). Plant viruses spread cell-to-cell by altering Pd. This movement scenario necessitates a targeting mechanism that delivers the virus to a Pd and a transport mechanism to move the virion or viral nucleic acid through the Pd channel. The identity of host proteins with which MP interacts, the mechanism
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Barash, Itamar, J. Mina Bissell, Alexander Faerman, and Moshe Shani. Modification of Milk Composition via Transgenesis: The Role of the Extracellular Matrix in Regulating Transgene Expression. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570558.bard.

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Altering milk composition via transgenesis depends on three main factors. (1) The availability of an efficient regulatory sequences for targeting transgene(s) to the mammary gland; (2) a reliable in vitro model to test the expression of transgenes prior to their introduction to the animal genome; and (3) better understanding of the major factors which determine the rate of gene expression and protein synthesis. The current studies provide the necessary means and knowledge to alter milk protein composition via transgenesis. The following specific goals were achieved: a: Identifying regulatory r
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Dawson, William O., and Moshe Bar-Joseph. Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7586540.bard.

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Citrus is one of the major agricultural crops common to Israel and the United States, important in terms of nutrition, foreign exchange, and employment. The economy of both citrus industries have been chronically plagued by diseases caused by Citrus tristeza virus (CTV). The short term solution until virus-resistant plants can be used is the use of mild strain cross-protection. We are custom designing "ideal" protecting viruses to immunize trees against severe isolates of CTV by purposely inoculating existing endangered trees and new plantings to be propagated as infected (protected) citrus bu
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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer
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