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1

Louis, Vanessa. "Human promoter characterization." DigitalCommons@Robert W. Woodruff Library, Atlanta University Center, 2010. http://digitalcommons.auctr.edu/dissertations/153.

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The basic-helix-loop-helix (bHLH) protein family plays an innate role in cellular activities. A pivotal member of this family, the Inhibitor of differentiation (Id) proteins, is linked to cell proliferation, differentiation, and tumorigenesis via inhibiting the DNA binding of bHLH proteins. There are four isoforms of Id genes - Id1-4 are uniquely expressed. In prostate cancer cells, Id3 is up-regulated and targets cellular proliferation and metastasis. However, the mechanism that triggers Id3 expression in prostate cancer is unknown. Therefore, a series of experiments were performed in order t
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2

Kilcup, Michael. "Characterization of the murine Unp promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32539.pdf.

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3

Ezpeleta, Jessica. "The characterization of the ABF-1 promoter." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/559.

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The basic helix-loop-helix (bHLH) family oftranscription factors consists of proteins involved in cellular proliferation and differentiation. The HLH structure plays a key role in protein-protein dimerization and with the DNA target sites, referred to as E boxes containing the consensus DNA sequence CANNTG. One class of mammalian class I bHLH proteins includes products of the E2A gene, which result from alternative splicing (E12, E47, and ITF), E2-2 and HEB. E2A proteins have also been detected in most cell lines with high levels of expression in lymphoid- and pancreatic cells. It has also bee
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4

Bodner, Caroline. "Identification and characterization of the murine Meis1 promoter." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=83966.

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MEIS1 (myeloid ecotropic viral integration site 1) is a member of the TALE class of homeodomain-containing proteins. MEIS1, along with related family members PBX and PREP1, bind DNA cooperatively with HOX proteins to regulate transcription. Meis1 is implicated in many developmental processes. Moreover, deregulation of Meis1 is involved in leukemogenesis. To better understand the mechanisms of Meis1 transcriptional regulation, we set out to map and characterize the Meis1 promoter. Results from RNase protection and 5' RACE suggest that Meis1 has multiple transcription start sites (TSSs) l
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5

Morris, Terry Lynn. "Molecular characterization of the fepA-fes bidirectional promoter in escherichia coli." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3025640.

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6

Jacobs, Sarah Margaret. "CHARACTERIZATION OF A PROMOTER REARRANGEMENT AND A SECOND PROMOTER IN THE HUMAN C-MYB PROTO-ONCOGENE." VCU Scholars Compass, 1993. http://scholarscompass.vcu.edu/etd/4938.

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The human cellular proto-oncogene c-myb has been implicated as important in the regulation of hematopoietic cell growth and differentiation. Aberrant expression of this gene and chromosomal aberrations near the c-myb locus have been associated with a number of carcinogenic processes. An alternatively spliced CDNA clone of c-myb, pMbm-2, contains unique 5’ sequences which replace exon 1. The existence of this 5’ divergent CDNA clone led us into a study of the promoter activity of the c-myb gene. Intron 1 of c-myb is highly conserved between human and mouse throughout the intron, while only thos
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7

Blundell, Matthew Charles. "Characterization of the promoter in a human B19 parvovirus." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/29011.

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The nucleotide sequence of the B19-Wi isolate of an autonomous human parvovirus was determined and compared with the sequence of the closely related isolate, B19-Au. The B19-Wi genome was similar to the B19-Au genome, as shown from DNA sequence analyses. It had been previously suggested from the sequence of the B19-Au genome, that the termini may be imperfect inverted terminal repeats. The additional sequence present on the right-hand terminus of B19-Wi supported that supposition. The hairpin termini of the B19 genome were of the same type as those found in adeno-associated parvoviruses, and s
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8

Chen, Zhiqi. "Cloning and characterization of the human CD200 promoter region." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ63146.pdf.

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9

Altmann, Stephen. "Molecular Characterization of Zebrafish Interferon, MX, and MX Promoter." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/AltmannSM2003.pdf.

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10

Johnson, Sabrina D. "Characterization of the Pichia pastoris alcohol oxidase I promoter." Scholarly Commons, 2003. https://scholarlycommons.pacific.edu/uop_etds/575.

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The methylotrophic yeast, Pichia past oris, is one of the most respected and widely used systems today. The ability of this yeast to produce large masses of protein and metabolize methanol as a sole source of carbon and energy is attributed to the highly induceable Alcohol Oxidase I promoter (AOXI). Despite of the disperse popularity and use of this promoter over the last 15 years, little is known about the transcription controls at a molecular level. A 5'>3' deletion analysis of the AOXI promoter was perrormed to gain understanding of the promoter's regulation and provided insight to the appr
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11

Penniket, Carolyn Renee. "Tissue-specific gene expression and promoter characterization in triticale." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2013, 2013. http://hdl.handle.net/10133/3373.

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Triticale (x Triticosecale Whitm.) is a cereal with favorable agronomic traits for a Canadian bioproduction platform crop. Appropriate tissue sampling times were determined and gene expression profiles were evaluated in five triticale seed tissues and eleven vegetative tissues using the Affymetrix Wheat GeneChip®. Genes that were expressed, not expressed, tissue-specific, tissue-enriched and developmentally regulated were identified. The percentage of probe sets on the wheat GeneChip with gene ontology annotations was improved from less than 3% to over 76% using homologous sequence identificat
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12

com, tdschoep@yahoo, and Tobias Delavilla Schoep. "Isolation and Characterization of Pseudobutyrivibrio ruminis Gene Promoters." Murdoch University, 2004. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050527.112003.

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A family of E. coli - P. ruminis shuttle-plasmids was constructed to allow the isolation and characterization of gene promoters from the rumen bacterium P. ruminis. The promoter rescue plasmid pBK was used to isolate a total of 4 genomic DNA fragments that promoted transcription in P. ruminis strains 0/10. These promoters, and an additional promoter, previously isolated from P. ruminis strain OR38 (Schoep, 1999), were identified by their ability to initiate expression of a promoterless ermAM gene in P. ruminis. Within 4 of the fragments, a total of 5 transcription start sites were identified i
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13

Chan, Tung-lei, and 陳冬妮. "Promoter characterization of testis specific protein, Y-linked like2 (TSPYL2)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41290410.

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14

Warshamana, Gnana Sakuntala. "Interactions of T7 RNA polymerase with its promoters : Part I: T7 promoter contacts essential for promoter activity in vivo ; Part II: Isolation and characterization of a mutant T7 RNA polymerase with altered promoter specificity." Diss., Georgia Institute of Technology, 1992. http://hdl.handle.net/1853/26303.

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15

De, Souza Rebecca Anne Grace. "Characterization of the Huntingtin gene promoter and Huntingtin transcriptional regulation." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/52951.

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Huntington’s disease (HD) is a late onset, neurological, autosomal dominant genetic disorder. Despite being associated to a defined genetic mutation within the huntingtin gene (HTT), little is known about its transcriptional regulation. HTT is expressed, at varying levels, throughout the body. At the current time, the transcriptional regulation mechanisms controlling this differential expression pattern are unknown. Previous studies have focused on the genomic region directly preceding HTT’s transcriptional start site. The purpose of this thesis was to utilize the current understanding of mamm
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16

Kim, Soo-yeon Sammy. "Characterization of a BMP responsive element in the Tlx2 promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/MQ46093.pdf.

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17

Jain, Nishant Rajkumar. "Characterization and functional analysis of the P2Y₂R gene promoter." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/4629.

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Thesis (M.S.)--University of Missouri-Columbia, 2006.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on April 21, 2009) Includes bibliographical references.
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18

Bianchi, Allison A. "Characterization and application of Escherichia coli stress promoter-lacZ fusions /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/8072.

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19

Chan, Tung-lei. "Promoter characterization of testis specific protein, Y-linked like 2 (TSPYL2)." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41290410.

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20

Rocha, Pedro. "Promoter trapping in Arabidopsis thaliana : characterization of T-DNA tagged lines." Thesis, University of Leicester, 1999. http://hdl.handle.net/2381/29798.

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Two A. thaliana lines transgenic for the promoter-trap vector pgusBin19 were studied. Line AtEN101 lacked a phenotype but reporter gene (gus) expression exhibited temporal and spatial regulation, including in the developing embryo. This pattern was unaltered when AtEN101 was used as a functional marker in two embryo-defective mutant backgrounds tested. In roots of AtEN101 seedlings, gus expression was repressed by ABA and modified by auxins, in a sucrose-modulated manner. The tagged genomic region in the mutant and wildtype, and cDNAs mapping to it were isolated. The cDNAs derived from two ove
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21

Li, Jack Jun. "Cloning and characterization of the rat bone sialoprotein (BSP) gene promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ35220.pdf.

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22

Patten, Cheryl L. "Isolation and characterization of a bacterial auxin gene and its promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ65255.pdf.

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23

Schlisner, Rebecca Joy. "Isolation and Characterization of the Y32G9A.8 Promoter in C. elegans." Digital Archive @ GSU, 2006. http://digitalarchive.gsu.edu/biology_theses/8.

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The over-expression of Down syndrome cell adhesion molecules (DSCAMs) is partially responsible for the mental retardation associated with Down syndrome. Previous work in our lab showed that a DSCAM homolog in C. elegans, Y32G9A.8, is expressed at all developmental stages and appears to be crucial for survival. In an effort to map the expression pattern, I used the Genome Sciences Centre’s primer design program (http://elegans.bcgsc.bc.ca/gfp_primers/) to design a GFP promoter fusion product that was used to monitor gene expression. The results indicate that Y32G9A.8 is expressed in the animal’
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24

Johnson, Sabrina D. "Characterization of the Pichia pastoris alcohol oxidase I promoter : a thesis." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/575.

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The methylotrophic yeast, Pichia past oris, is one of the most respected and widely used systems today. The ability of this yeast to produce large masses of protein and metabolize methanol as a sole source of carbon and energy is attributed to the highly induceable Alcohol Oxidase I promoter (AOXI). Despite of the disperse popularity and use of this promoter over the last 15 years, little is known about the transcription controls at a molecular level. A 5'>3' deletion analysis of the AOXI promoter was perrormed to gain understanding of the promoter's regulation and provided insight to the appr
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25

Rife, Terrie K. "The characterization of a Human Nitric Oxide Synthase I Promoter Complex /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488192119262512.

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26

Zhang, Shuang. "Tandem Affinity Purification of Myogenin and Characterization of the Tcap Promoter." OpenSIUC, 2010. https://opensiuc.lib.siu.edu/theses/205.

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Myogenesis is orchestrated by a family of four related bHLH transcription factors; MyoD, Myf5, Myogenin and Mrf4. The MRFs form heterodimers with E proteins and bind to the promoters or enhancers of muscle specific genes. Of these four, the Myogenic Regulatory Factor (MRF), myogenin, has been shown to play an important role during terminal differentiation of skeletal muscle. However, little is known about myogenin's function during myogenesis. In order to reveal myogenin associated factors, we performed tandem affinity purification of myogenin. The TAP purification with HEK293 cells elucidate
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27

Gurney, Austin Louis. "Characterization of the promoter region of the gene for phosphoenolpyruvate carboxykinase (GTP)." Case Western Reserve University School of Graduate Studies / OhioLINK, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=case1056548533.

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28

Gao, Jinxin. "Regulatory elements in the Staphylococcus aureus protein A (Spa) gene promoter and characterization of SarS binding to the Spa promoter /." Search for this dissertation online, 2004. http://wwwlib.umi.com/cr/ksu/main.

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29

Cai, Xuediao. "Synthesis and Characterization of Pyrrole Based Adhesion Promoter Systems on Oxide Substrates." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1109334028224-37122.

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For grafting polypyrrole on oxidized surfaces, 3-substituted pyrrole alkyl phosphonic acids, 11-(pyrrol-3-yl undecyl) trimethoxysilanes and 1-substituted pyrrole alkyl organosilanes with different chain length were designed and successfully synthesized as adhesion promoters. These new derivatives were studied for their adsorption behavior on oxide substrates and chemical or electrochemical deposition of polypyrrole over modified oxide surface or electrodes. Several analytical techniques such as contact angle measurement, surface plasmon resonance spectroscopy (SPR), UV-VIS Spectroscopy, grazin
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30

Karantzoulis-Fegaras, Fotula. "Molecular characterization of the proximal human endothelial nitric oxide synthase (eNOS) promoter." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0021/NQ45733.pdf.

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31

Chu, Ying-ying Jamie. "Characterization of the promoter of dehalogenase IVa gene of Burkholderia sp. MBA4." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37840150.

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32

Chu, Ying-ying Jamie, and 朱盈盈. "Characterization of the promoter of dehalogenase IVa gene of Burkholderia sp. MBA4." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37840150.

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33

Cai, Xuediao. "Synthesis and Characterization of Pyrrole Based Adhesion Promoter Systems on Oxide Substrates." Doctoral thesis, Technische Universität Dresden, 2004. https://tud.qucosa.de/id/qucosa%3A24463.

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For grafting polypyrrole on oxidized surfaces, 3-substituted pyrrole alkyl phosphonic acids, 11-(pyrrol-3-yl undecyl) trimethoxysilanes and 1-substituted pyrrole alkyl organosilanes with different chain length were designed and successfully synthesized as adhesion promoters. These new derivatives were studied for their adsorption behavior on oxide substrates and chemical or electrochemical deposition of polypyrrole over modified oxide surface or electrodes. Several analytical techniques such as contact angle measurement, surface plasmon resonance spectroscopy (SPR), UV-VIS Spectroscopy, grazin
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34

Baek, Daehyun. "Characterization of evolutionarily conserved mammalian alternative splicing and alternative promoters /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/8036.

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35

Lefever, Mark. "DESIGN, SYNTHESIS AND CHARACTERIZATION OF HELICAL OPIOID GLYCOPEPTIDES AND FLUORESCENT DERIVATIVES INCLUDING OPTIMIZATION OF SERINE GLYCOSYLATION UTILIZING SUGAR ACETATES." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/193791.

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Our effort to provide an efficient route to serine glycosides with utility in glycopeptide synthesis has led to the identification of two particularly effective promoters of O-glycosylation. Indium(III) bromide and scandium(III) triflate were shown to be superior promoters of microwave accelerated O-glycosylation utilizing peracetyl carbohydrate donors. 247, 249 These Lewis acids afforded several advantages over previously described promoters including, increased yields, tolerance to moisture, decreased environmental toxicity, ease of work up, and increased reproducibility. Both affected the m
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36

Mohd, Fazli Bin Farida Asras. "Molecular breeding and biochemical characterization of an oleaginous fungus Mortierella alpina for prostaglandin F2α production". Kyoto University, 2019. http://hdl.handle.net/2433/242698.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第21821号<br>農博第2334号<br>新制||農||1067(附属図書館)<br>学位論文||H31||N5193(農学部図書室)<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授 小川 順, 教授 植田 充美, 教授 栗原 達夫<br>学位規則第4条第1項該当
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37

Rawat, Reetika. "Characterization of the promoter of SmCP, the gene encoding Solanum melongena cysteine proteinase." Thesis, Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B34740156.

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38

Little, Natasha W. "Characterization of IAPLTR1 subclasses and bidirectional promoter activity : "making sense of it all"." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/61086.

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Endogenous retroviruses (ERVs) are over five-times more prevalent than gene coding sequences in the mouse and human genomes (5). The long terminal repeats (LTRs) of these elements are promoter-enhancers that can have many regulatory effects on the host genome (3). Intracisternal A-Particles (IAPs) are a highly active, murine-specific class of ERV that is known to have strong LTR-driven promoter activity (10). In a recent study, a sequence divergence-based subclass nomenclature was suggested for several classes of IAPLTR – including IAPLTR1 (59). However, it remained unknown whether these high
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39

de, Belle Ian D. "Characterization of c-fos promoter binding proteins in normal and neoplastic liver cells." Thesis, University of Ottawa (Canada), 1995. http://hdl.handle.net/10393/10392.

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The ability of the c-fos gene to respond to a variety of stimuli reflects the complexity of its regulation. Indeed, aberrant regulation can lead to cellular transformation. The fine regulation of the gene relies on the interaction of protein factors interacting with a set of regulatory elements present within the promoter of the gene in a cooperative fashion. In these studies, multiprotein complexes capable of binding to the serum response element, the cAMP response element, and sis-conditioned medium response element, were identified in liver cell nuclei. A subset of these proteins was able t
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40

Paramaswara, Bamini. "Characterization of the promoter for the human factor I (C3b/C4b inactivator) gene." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ34077.pdf.

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41

Zarrin, Ali Akbar. "Characterization of the human recombination activating gene 1 (RAG1) and RAG2 promoter regions." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0024/NQ49915.pdf.

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42

Lai, King-yin. "Cloning and characterization of the demilune cells and parotid protein (Dcpp) gene promoter /." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36433834.

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43

鄭智強 and Chi-keung Cheng. "Structural organization of the mouse testin gene and characterization of its promoter sequence." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31224106.

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44

Lai, King-yin, and 賴景然. "Cloning and characterization of the demilune cells and parotid protein(Dcpp) gene promoter." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45010821.

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45

Wright, Kenneth Lynn. "Functional and Structural Characterization of a Human H4 Histone Gene Promoter: a Thesis." eScholarship@UMMS, 1990. https://escholarship.umassmed.edu/gsbs_diss/52.

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Expression of the cell cycle dependent FO10S human H4 histone gene is regulated at both the transcriptional and post-transcriptional levels. We have investigated the 5' promoter elements mediating the transcriptional aspects of its regulation. A detailed in vivo and in vitro transcriptional analysis of promoter deletion mutants from this gene has identified three positive regulatory elements and two potentially negative regulatory elements within the first 1000 base pairs upstream of the transcription initiation site. In addition, the minimal promoter located within the first 70 base pairs is
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46

Zukunft, Jörg. "Functional characterization of promoter polymorphisms in the human Cytochrome P450 2B6 gene (CYP2B6)." [S.l. : s.n.], 2005. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB11947836.

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47

Cheng, Chi-keung. "Structural organization of the mouse testin gene and characterization of its promoter sequence." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22424763.

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48

Stein, Marcello Francesco [Verfasser], and Lars [Akademischer Betreuer] Nitschke. "Characterization of the CD83 promoter, enhancer complex / Marcello Francesco Stein. Betreuer: Lars Nitschke." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2011. http://d-nb.info/1016377185/34.

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49

"Characterization of acetylcholinesterase and its promoter region in Tetraodon nigroviridis." 2006. http://library.cuhk.edu.hk/record=b5892899.

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Lau Suk Kwan.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2006.<br>Includes bibliographical references (leaves 128-150).<br>Abstracts in English and Chinese.<br>Acknowledgment --- p.i<br>Table of content --- p.ii<br>List of Figures --- p.x<br>List of Tables --- p.xiv<br>Abbreviation --- p.xv<br>Abstract --- p.xviii<br>論文摘要 --- p.xx<br>Chapter 1 --- Chapter 1 Introduction --- p.1<br>Chapter 1.1 --- Tetraodon nigroviridis --- p.1<br>Chapter 1.1.1 --- Background --- p.1<br>Chapter 1.1.2 --- Genomic Sequencing Project --- p.3<br>Chapter 1.1.3 --- Tetraodon nigroviridis as Stud
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50

Annala, Katja. "Characterization of promoter regions for design of a cell specific promoter." Thesis, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-340861.

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Global warming is one of the modern world’s biggest problems and renewable fuels are needed to meet this challenge. Biohydrogen is one alternative of a renewable fuel and can be produced from genetically modified cyanobacteria. Although the production is not yet efficient enough to compete with fossil fuels on a commercial level, it has potential. By gaining knowledge regarding pathways and regulatory mechanisms in cyanobacteria, genetic tools can be built and further enhance the production of biohydrogen. A synthetic promoter, which is active only in hydrogen producing cells, heterocysts, is
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