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Journal articles on the topic 'Promoteur 35S'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Govindarajulu, Manjula, James M. Elmore, Thomas Fester, and Christopher G. Taylor. "Evaluation of Constitutive Viral Promoters in Transgenic Soybean Roots and Nodules." Molecular Plant-Microbe Interactions® 21, no. 8 (2008): 1027–35. http://dx.doi.org/10.1094/mpmi-21-8-1027.

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The efficiency of β-glucuronidase (GUS) expression was evaluated with five viral promoters to identify the most suitable promoter or promoters for use in soybean hairy roots, including applications to study the symbiotic interaction with Bradyrhizobium japonicum. Levels of GUS activity were fluorimetrically and histochemically assayed when the GUS (uidA) gene was driven by the Cauliflower mosaic virus (CaMV) 35S promoter and enhanced 35S (E35S) promoter, the Cassava vein mosaic virus (CsVMV) promoter, the Figwort mosaic virus (FMV) promoter, and the Strawberry vein banding virus (SVBV2) promot

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2

Song, Guo-Qing, Kenneth C. Sink, Peter W. Callow, Rebecca Baughan, and James F. Hancock. "Evaluation of a Herbicide-resistant Trait Conferred by the Bar Gene Driven by Four Distinct Promoters in Transgenic Blueberry Plants." Journal of the American Society for Horticultural Science 133, no. 4 (2008): 605–11. http://dx.doi.org/10.21273/jashs.133.4.605.

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Four chimeric bialaphos resistance (bar) genes driven by different promoters were evaluated for production of herbicide-resistant ‘Legacy’ blueberry plants (73.4% Vaccinium corymbosum L. and 25% Vaccinium darrowi Camp) through Agrobacterium tumefaciens (Smith & Towns.) Conn.-mediated transformation. When the bars were used as selectable marker genes, different promoters yielded different transformation frequencies. Three chimeric bar genes with the promoter nopaline synthase (nos), cauliflower mosaic virus (CaMV) 35S, or CaMV 34S yielded transgenic plants, whereas a synthetic (Aocs)3AmasPm

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3

Castañón, S., M. S. Marín, J. M. Martín-Alonso, et al. "Immunization with Potato Plants Expressing VP60 Protein Protects against Rabbit Hemorrhagic Disease Virus." Journal of Virology 73, no. 5 (1999): 4452–55. http://dx.doi.org/10.1128/jvi.73.5.4452-4455.1999.

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ABSTRACT The major structural protein VP60 of rabbit hemorrhagic disease virus (RHDV) has been produced in transgenic potato plants under the control of a cauliflower mosaic virus 35S promoter or a modified 35S promoter that included two copies of a strong transcriptional enhancer. Both types of promoters allowed the production of specific mRNAs and detectable levels of recombinant VP60, which were higher for the constructs carrying the modified 35S promoter. Rabbits immunized with leaf extracts from plants carrying this modified 35S promoter showed high anti-VP60 antibody titers and were full

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4

Miroshnichenko, Dmitry, Aleksey Firsov, Vadim Timerbaev, et al. "Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato." Plants 9, no. 11 (2020): 1520. http://dx.doi.org/10.3390/plants9111520.

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Various plant-derived promoters can be used to regulate ectopic gene expression in potato. In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) gene. The strengths of StUbi, StGBSS, StPat, and StLhca3 promoters were compared with the conventional constitutive CaMV 35S promoter in various organs (leaves, stems, roots, and tubers) of greenhouse-grown plants. The final amount of gene product was determined at the post-transcriptional level using histochemica

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Brehm, Ilka, Regina Preisig-Müller, and Helmut Kindl. "Grapevine Protoplasts as a Transient Expression System for Comparison of Stilbene Synthase Genes Containing cGMP-Responsive Promoter Elements." Zeitschrift für Naturforschung C 54, no. 3-4 (1999): 220–29. http://dx.doi.org/10.1515/znc-1999-3-412.

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Abstract A method for preparing elicitor-responsive protoplasts from grapevine cells kept in suspension culture was established. The protoplasts were employed in order to perform transient gene expression experiments produced by externally added plasmids. Using the gene coding for bacterial β-glucuronidase as the reporter gene, the transient expression under the control of various promoters of stilbene synthase genes were analyzed. The elicitor-responsiveness of promoters from grapevine genes and heterologous promoters were assayed: the grapevine stilbene synthase gene VST-1 and pine stilbene

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6

Sanfaçon, Hélène. "Regulation of mRNA formation in plants: lessons from the cauliflower mosaic virus transcription signals." Canadian Journal of Botany 70, no. 5 (1992): 885–99. http://dx.doi.org/10.1139/b92-113.

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The cauliflower mosaic virus (CaMV) transcription signals are common tools of plant molecular biologists. In this article, the transcription signals are discussed in light of the life cycle of CaMV, a plant pararetrovirus. Production of mature 35S RNA, the terminally redundant genomic RNA, is regulated by the 35S promoter, a very strong promoter, and by the polyadenylation signal that is present twice on the RNA but recognized only at its 3′ end. Dissection of the promoter has identified several organ-specific elements acting in concert to express the 35S RNA in most plant cells. Studies on th

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7

Last, David I., and Danny J. Llewellyn. "A detoxification gene in transgenicNicotiana tabacumconfers 2,4-D tolerance." Weed Science 47, no. 4 (1999): 401–4. http://dx.doi.org/10.1017/s0043174500091980.

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TransgenicNicotiana tabacumwith tolerance to 2,4-D has previously been produced using a bacterial 2,4-D-dioxygenase gene (tfdA) driven by the 35S promoter of cauliflower mosaic virus. Using promoters from thePisum sativumplastocyanin gene (petE) and anArabidopsis thalianahistone gene (H4A), we demonstrate that similar protection from 2,4-D can be obtained in transgenicN. tabacumby targeting expression oftfdAto either meristematic tissues or chloroplast-containing tissues. As with the 35S promoter constructs, the plants are tolerant but not completely resistant; very young seedlings in particul

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8

Smith*, Alan G., Nicole Gardner, and Tracy A. Melberg. "Differential Expression of rolC Results in Unique Plant Phenotypes." HortScience 39, no. 4 (2004): 756C—756. http://dx.doi.org/10.21273/hortsci.39.4.756c.

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As residential lot sizes decrease, there is an increased demand for new, small-statured landscape plants to fit into the smaller lots. One promising method to create smaller plants is by introducing a dwarfing gene into a plant of interest. A dwarfing gene that has been identified is the rolC gene from Agrobacterium rhizogenes. Expression of rolC in plants has been shown to cause decreased height and internode length, increased branching, and modified leaf size in a several species. Although the effects of the rolC gene have been well characterized for many plant species, most research has con

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9

Pauli, Sandra, Helen M. Rothnie, Gang Chen, Xiaoyuan He, and Thomas Hohn. "The Cauliflower Mosaic Virus 35S Promoter Extends into the Transcribed Region." Journal of Virology 78, no. 22 (2004): 12120–28. http://dx.doi.org/10.1128/jvi.78.22.12120-12128.2004.

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ABSTRACT A 60-nucleotide region (S1) downstream of the transcription start site of the cauliflower mosaic virus 35S RNA can enhance gene expression. By using transient expression assays with plant protoplasts, this activity was shown to be at least partially due to the effect of transcriptional enhancers within this region. We identify sequence motifs with enhancer function, which are normally masked by the powerful upstream enhancers of the 35S promoter. A repeated CT-rich motif is involved both in enhancer function and in interaction with plant nuclear proteins. The S1 region can also enhanc

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10

PRADHAN, Sriharsa, Nigel A. R. URWIN, Gareth I. JENKINS, and Roger L. P. ADAMS. "Effect of CWG methylation on expression of plant genes." Biochemical Journal 341, no. 3 (1999): 473–76. http://dx.doi.org/10.1042/bj3410473.

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The presence of two DNA methyltransferases in Pisum raises the possibility that they serve different functions. In vitro methylation of CWG sequences in the strong cauliflower mosaic virus 35S promoter had no effect on reporter gene expression. In contrast, in vitro methylation of CWG sequences in the relatively weak, CG-deficientPhaseolus vulgaris rbcS2 promoter inhibited transcription. Expression of both constructs was strongly inhibited by extensive CG methylation. A search of published plant promoter sequences revealed that the CG content of promoters is very variable, with some promoters

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SOROKIN, ANATOLY A., ALEXANDR A. OSYPOV, TIMUR R. DZHELYADIN, PETR M. BESKARAVAINY та SVETLANA G. KAMZOLOVA. "ELECTROSTATIC PROPERTIES OF PROMOTER RECOGNIZED BYE. COLIRNA POLYMERASE Eσ70". Journal of Bioinformatics and Computational Biology 04, № 02 (2006): 455–67. http://dx.doi.org/10.1142/s0219720006002077.

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A comparative analysis of electrostatic patterns for 359 σ70-specific promoters and 359 nonpromoter regions on electrostatic map of Escherichia coli genome was carried out. It was found that DNA is not a uniformly charged molecule. There are some local inhomogeneities in its electrostatic profile which correlate with promoter sequences. Electrostatic patterns of promoter DNAs can be specified due to the presence of some distinctive motifs which differ for different promoter groups and may be involved as signal elements in differential recognition of various promoters by the enzyme. Some specif

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Varchenko, O. I., B. M. Krasyuk, A. A. Fedchunov, O. V. Zimina, M. F. Parii, and Yu V. Symonenko. "Genetic constructs creation using Golden Gate cloning method." Faktori eksperimental'noi evolucii organizmiv 25 (August 30, 2019): 190–96. http://dx.doi.org/10.7124/feeo.v25.1163.

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Aim. Creation of genetic constructions to study the effects of various regulatory elements, namely promoters, on the expression of GFP reporter protein. Methods. For creation genetic constructs, the method of molecular cloning Golden Gate was used, which allows the rapid creation of genetic vectors using IIS type restriction enzymes and T4 DNA liga-ses. Results. For research six different promoters were selected, namely the 35S CaMV (Cauliflower Mosaic Virus), double 35S CaMV promoter, promoters of the RbcS2B and RbcS1B genes encoding a small subunit of ribulozobisphosphate carboxylase (RuBisC

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13

Ma, Hongmei, Margaret Pooler, and Robert Griesbach. "Ratio of Myc and Myb Transcription Factors Regulates Anthocyanin Production in Orchid Flowers." Journal of the American Society for Horticultural Science 133, no. 1 (2008): 133–38. http://dx.doi.org/10.21273/jashs.133.1.133.

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Many studies have examined anthocyanin gene expression in colorless tissues by introducing anthocyanin regulatory genes of the MYC/R and MYB/C1 families. Expression of the two regulatory genes under the control of a strong promoter generally results in high anthocyanin accumulation. However, such approaches usually have a negative effect on growth and development of the recovered plants. In this study the author used two promoters of different strengths—a weak (Solanum tuberosum L. polyubiquitin Ubi3) and a strong (double 35S) promoter—and generated two sets of expression constructs with the Z

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14

Ho, Mae-Wan, and Joe Cummins. "New evidence links CaMV 35S promoter to HIV transcription." Microbial Ecology in Health and Disease 21, no. 3-4 (2009): 172–74. http://dx.doi.org/10.3109/08910600903495053.

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15

Okumura, Azusa, Asahi Shimada, Satoshi Yamasaki, et al. "CaMV-35S promoter sequence-specific DNA methylation in lettuce." Plant Cell Reports 35, no. 1 (2015): 43–51. http://dx.doi.org/10.1007/s00299-015-1865-y.

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16

Hille, Annette, Akua Badu-Antwi, Daniela Holzer, and Friedrich A. Grässer. "Lysine residues of Epstein–Barr virus-encoded nuclear antigen 2 do not confer secondary modifications via ubiquitin or SUMO-like proteins but modulate transcriptional activation." Journal of General Virology 83, no. 5 (2002): 1037–42. http://dx.doi.org/10.1099/0022-1317-83-5-1037.

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Epstein–Barr virus nuclear antigen 2 (EBNA2) is essential for transformation through activation of viral and cellular genes. Within 487 residues, EBNA2 contains six lysine (K) residues (positions 335, 357, 359, 363, 366 and 480), which were mutated to arginine (R) residues, either individually or in combination, and tested for subcellular localization, mobility by SDS–PAGE and transactivation of three promoters. All mutants featuring the K480R mutation within the nuclear localization signal were partially cytoplasmic with a reduced level of transactivation of the latent membrane protein 1 (LMP

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17

Myers, J. Michele, and Philipp W. Simon. "Optimization of Parameters for Particle Bombardment of Genes to Garlic." HortScience 31, no. 4 (1996): 616c—616. http://dx.doi.org/10.21273/hortsci.31.4.616c.

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We evaluated the efficiency of transformation in garlic for promoter activity, osmoticum effect and shaker speed using particle bombardment as the method of gene delivery. Callus was produced from root segments on a modified B-5 medium for four garlic clones. Suspension cultures were then established on a modified B-5 medium + 2,4-D using 6-month-old callus. Cells were collected by vacuum filtration and the Bio-Rad PDS-1000/He system was used to deliver genes. The activities of CaMV 35S, maize Adh1, and rice Act promoters were evaluated for transient expression using the β-glucuronidase (GUS)

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18

Galli, Mary, Angie Theriault, Dong Liu, and Nigel M. Crawford. "Expression of the Arabidopsis Transposable Element Tag1 Is Targeted to Developing Gametophytes." Genetics 165, no. 4 (2003): 2093–105. http://dx.doi.org/10.1093/genetics/165.4.2093.

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Abstract The Arabidopsis transposon Tag1 undergoes late excision during vegetative and germinal development in plants containing 35S-Tag1-GUS constructs. To determine if transcriptional regulation can account for the developmental control of Tag1 excision, the transcriptional activity of Tag1 promoter-GUS fusion constructs of various lengths was examined in transgenic plants. All constructs showed expression in the reproductive organs of developing flowers but no expression in leaves. Expression was restricted to developing gametophytes in both male and female lineages. Quantitative RT-PCR ana

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19

Schornack, Sebastian, Kristin Peter, Ulla Bonas, and Thomas Lahaye. "Expression Levels of avrBs3-Like Genes Affect Recognition Specificity in Tomato Bs4- But Not in Pepper Bs3-Mediated Perception." Molecular Plant-Microbe Interactions® 18, no. 11 (2005): 1215–25. http://dx.doi.org/10.1094/mpmi-18-1215.

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The tomato Bs4 disease resistance gene mediates recognition of avrBs4-expressing strains of the bacterial spot pathogen Xanthomonas campestris pv. vesicatoria to give a hypersensitive response (HR). Here, we present the characterization of the Bs4 promoter and its application for lowlevel expression of bacterial type III effector proteins in planta. Real-time polymerase chain reaction showed that Bs4 is constitutively expressed at low levels and that transcript abundance does not change significantly upon infection with avrBs4-containing xanthomonads. A 302-bp promoter fragment was found to be

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20

Shirasawa-Seo, Naomi, Yoshitaka Sano, Shigeo Nakamura, et al. "Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco." Journal of General Virology 86, no. 6 (2005): 1851–60. http://dx.doi.org/10.1099/vir.0.80790-0.

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Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level o

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SHIRASAWA-SEO, Naomi, Ichiro MITSUHARA, Shigeo NAKAMURA та ін. "Constitutive Promoters Available for Transgene Expression Instead of CaMV 35S RNA Promoter : Arabidopsis Promoters of Tryptophan Synthase Protein β Subunit and Phytochrome B". Plant Biotechnology 19, № 1 (2002): 19–26. http://dx.doi.org/10.5511/plantbiotechnology.19.19.

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22

Efremova, Larisa N., Svetlana R. Strelnikova, Guzel R. Gazizova, Elena A. Minkina, and Roman A. Komakhin. "A Synthetic Strong and Constitutive Promoter Derived from the Stellaria media pro-SmAMP1 and pro-SmAMP2 Promoters for Effective Transgene Expression in Plants." Genes 11, no. 12 (2020): 1407. http://dx.doi.org/10.3390/genes11121407.

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Synthetic promoters are vital for genetic engineering-based strategies for crop improvement, but effective methodologies for their creation and systematic testing are lacking. We report here on the comparative analysis of the promoters pro-SmAMP1 and pro-SmAMP2 from Stellaria media ANTIMICROBIAL PEPTIDE1 (AMP1) and ANTIMICROBIAL PEPTIDE2 (AMP2). These promoters are more effective than the well-known Cauliflower mosaic virus 35S promoter. Although these promoters share about 94% identity, the pro-SmAMP1 promoter demonstrated stronger transient expression of a reporter gene in Agrobacterium infi

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Loopstra, Carol A., Arthur K. Weissinger, and Ronald R. Sederoff. "Transient gene expression in differentiating pine wood using microprojectile bombardment." Canadian Journal of Forest Research 22, no. 7 (1992): 993–96. http://dx.doi.org/10.1139/x92-133.

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We have used microprojectile bombardment to obtain transient expression of the reporter gene β-glucuronidase in differentiating wood (secondary xylem) of loblolly pine (Pinustaeda L.), thereby providing a method for studying expression of introduced DNA in this important tissue. β-Glucuronidase activity can be observed in different cell types, including tracheids, ray parenchyma, and axial parenchyma associated with resin canals. Microprojectile bombardment can be used to identify active promoters and to compare the relative activities of different promoters in specific cell types. We have stu

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Buckner, Cindy M., та Charles P. Moran. "A Region in Bacillus subtilisςH Required for Spo0A-Dependent Promoter Activity". Journal of Bacteriology 180, № 18 (1998): 4987–90. http://dx.doi.org/10.1128/jb.180.18.4987-4990.1998.

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ABSTRACT Spo0A activates transcription in Bacillus subtilis from promoters that are used by two types of RNA polymerase, RNA polymerase containing the primary sigma factor, ςA, and RNA polymerase containing a secondary sigma factor, known as ςH. The region of ςA near positions 356 to 359 is required for Spo0A-dependent promoter activation, possibly because Spo0A interacts with this region of ςA at these promoters. To determine if the amino acids in the corresponding region of ςH are also important in Spo0A-dependent promoter activation, we examined the effects of single alanine substitutions a

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Yamasaki, Satoshi, Masayuki Oda, Hiroyuki Daimon, et al. "Epigenetic modifications of the 35S promoter in cultured gentian cells." Plant Science 180, no. 4 (2011): 612–19. http://dx.doi.org/10.1016/j.plantsci.2011.01.008.

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Kasahara, Koji, Kazushige Ohtsuki, Sewon Ki, et al. "Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae." Molecular and Cellular Biology 27, no. 19 (2007): 6686–705. http://dx.doi.org/10.1128/mcb.00876-07.

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ABSTRACT HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1

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Dong, Liu, Fan, et al. "The Artificial Promoter rMdAG2I Confers Flower-specific Activity in Malus." International Journal of Molecular Sciences 20, no. 18 (2019): 4551. http://dx.doi.org/10.3390/ijms20184551.

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Genetic modifications of floral organs are important in the breeding of Malus species. Flower-specific promoters can be used to improve floral organs specifically, without affecting vegetative organs, and therefore developing such promoters is highly desirable. Here, we characterized two paralogs of the Arabidopsis thaliana gene AGAMOUS (AG) from Malus domestica (apple): MdAG1 and MdAG2. We then isolated the second-intron sequences for both genes, and created four artificial promoters by fusing each intron sequence to a minimal 35S promoter sequence in both the forward and reverse directions.

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Lipp, M., R. Schilling, S. Wiest, G. Laux, and G. W. Bornkamm. "Target sequences for cis-acting regulation within the dual promoter of the human c-myc gene." Molecular and Cellular Biology 7, no. 4 (1987): 1393–400. http://dx.doi.org/10.1128/mcb.7.4.1393.

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Recombinant plasmids of the human c-myc promoter-leader region and the bacterial chloramphenicol acetyltransferase (cat) gene were constructed. After transfection into different rodent and human cells, the 862-base-pair (bp) PvuII fragment carrying both c-myc promoters and 350 bp of the untranslated leader conferred 1/15 to 1/30 of the CAT activity mediated by the simian virus 40 promoter. The presence of additional sequences upstream of the PvuII fragment had an overall negative effect on c-myc promoter activity detectable by titration analysis with small amounts of transfected plasmid DNA. T

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Lipp, M., R. Schilling, S. Wiest, G. Laux, and G. W. Bornkamm. "Target sequences for cis-acting regulation within the dual promoter of the human c-myc gene." Molecular and Cellular Biology 7, no. 4 (1987): 1393–400. http://dx.doi.org/10.1128/mcb.7.4.1393-1400.1987.

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Recombinant plasmids of the human c-myc promoter-leader region and the bacterial chloramphenicol acetyltransferase (cat) gene were constructed. After transfection into different rodent and human cells, the 862-base-pair (bp) PvuII fragment carrying both c-myc promoters and 350 bp of the untranslated leader conferred 1/15 to 1/30 of the CAT activity mediated by the simian virus 40 promoter. The presence of additional sequences upstream of the PvuII fragment had an overall negative effect on c-myc promoter activity detectable by titration analysis with small amounts of transfected plasmid DNA. T

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Bertioli, David J., Matthew Smoker, and Paul R. Burrows. "Nematode-Responsive Activity of the Cauliflower Mosaic Virus 35S Promoter and Its Subdomains." Molecular Plant-Microbe Interactions® 12, no. 3 (1999): 189–96. http://dx.doi.org/10.1094/mpmi.1999.12.3.189.

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Root-knot and cyst nematodes are obligate plant parasites that induce complex biotrophic feeding structures in host roots. The mechanisms by which nematodes regulate host gene expression to produce feeding sites are unknown. The cauliflower mosaic virus (CaMV) 35S promoter has been reported to be repressed strongly in the feeding sites of both root-knot and cyst nematodes. In contrast, other work has indicated that this promoter is partially active in some feeding sites. Considering the importance of the 35S promoter in biotechnology, we have defined the nematoderesponsive nature of this promo

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Feng, Zhengyan, Zhengjing Zhang, Kai Hua, et al. "A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis." International Journal of Molecular Sciences 19, no. 12 (2018): 3925. http://dx.doi.org/10.3390/ijms19123925.

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The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous plant species. In Arabidopsis, constitutive promoters usually result in a low efficiency of heritable mutation in the T1 generation. In this work, CRISPR/Cas9 gene editing efficiencies using different promoters to drive Cas9 expression were evaluated. Expression of Cas9 under the constitutive CaMV 35S promoter resulted in a 2.3% mutation rate in T1 plants and failed to produce homozygous mutations in the T1 and T2 generations. In contrast, expression of Cas9 under two cell division-specific promoters, YAO and C

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Tzean, Yuh, Ho-Hsiung Chang, Tsui-Chin Tu, et al. "Engineering Plant Resistance to Tomato Yellow Leaf Curl Thailand Virus Using a Phloem-Specific Promoter Expressing Hairpin RNA." Molecular Plant-Microbe Interactions® 33, no. 1 (2020): 87–97. http://dx.doi.org/10.1094/mpmi-06-19-0158-r.

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Transgenic approaches employing RNA interference (RNAi) strategies have been successfully applied to generate desired traits in plants; however, variations between RNAi transgenic siblings and the ability to quickly apply RNAi resistance to diverse cultivars remain challenging. In this study, we assessed the promoter activity of a cauliflower mosaic virus 35S promoter (35S) and a phloem-specific promoter derived from rice tungro bacilliform virus (RTBV) and their efficacy to drive RNAi against the endogenous glutamate-1-semialdehyde aminotransferase gene (GSA) that acts as a RNAi marker, throu

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Mestel, R., M. Yip, J. P. Holland, E. Wang, J. Kang, and M. J. Holland. "Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities." Molecular and Cellular Biology 9, no. 3 (1989): 1243–54. http://dx.doi.org/10.1128/mcb.9.3.1243.

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Sequences within the spacer region of yeast rRNA cistrons stimulate synthesis of the major 35S rRNA precursor in vivo 10- to 30-fold (E. A. Elion and J. R. Warner, Cell 39:663-673, 1984). Spacer sequences that mediate this stimulatory activity are located approximately 2.2 kilobases upstream from sequences that encode the 5' terminus of the 35S rRNA precursor. By utilizing a centromere-containing plasmid carrying a 35S rRNA minigene, a 160-base-pair region of spacer rDNA was identified by deletion mapping that is required for efficient stimulation of 35S rRNA synthesis in vivo. A 22-base-pair

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Mestel, R., M. Yip, J. P. Holland, E. Wang, J. Kang, and M. J. Holland. "Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities." Molecular and Cellular Biology 9, no. 3 (1989): 1243–54. http://dx.doi.org/10.1128/mcb.9.3.1243-1254.1989.

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Sequences within the spacer region of yeast rRNA cistrons stimulate synthesis of the major 35S rRNA precursor in vivo 10- to 30-fold (E. A. Elion and J. R. Warner, Cell 39:663-673, 1984). Spacer sequences that mediate this stimulatory activity are located approximately 2.2 kilobases upstream from sequences that encode the 5' terminus of the 35S rRNA precursor. By utilizing a centromere-containing plasmid carrying a 35S rRNA minigene, a 160-base-pair region of spacer rDNA was identified by deletion mapping that is required for efficient stimulation of 35S rRNA synthesis in vivo. A 22-base-pair

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Dutt, Manjul, Zhijian T. Li, Sadanand Dhekney, and Dennis J. Gray. "(285) Characterization of a Composite Promoter from Genomic Sequences of Grapevine." HortScience 41, no. 4 (2006): 1053C—1053. http://dx.doi.org/10.21273/hortsci.41.4.1053c.

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Genetic transformation of plants necessitates the use of promoters to control transgene expression. Numerous promoters have been isolated from a wide range of organisms for use in plants. However, many of these natural promoters exhibit relatively low activity and/or have limited use. To provide an alternative, we constructed a composite promoter (EP) using a genomic DNA sequence and a 35 bp TATA-containing fragment from the 2S albumin (VvAlb1) gene core promoter of grapevine. The 0.9-kb genomic sequence was identified after TAIL-PCR, based on the presence of several unique cis-acting elements

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Li, Dandan, Rucong Xu, Dong Lv, et al. "Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter." International Journal of Molecular Sciences 21, no. 6 (2020): 1909. http://dx.doi.org/10.3390/ijms21061909.

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The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of diff

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Prat, Salomé, L. Willmitzer, and Jose J. Sánchez-Serrano. "Nuclear proteins binding to a cauliflower mosaic virus 35S truncated promoter." Molecular and General Genetics MGG 217, no. 2-3 (1989): 209–14. http://dx.doi.org/10.1007/bf02464883.

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Lam, Eric, and Nam-Hai Chua. "ASF-2: A Factor That Binds to the Cauliflower Mosaic Virus 35S Promoter and a Conserved GATA Motif in Cab Promoters." Plant Cell 1, no. 12 (1989): 1147. http://dx.doi.org/10.2307/3868912.

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Lam, E., and N. H. Chua. "ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters." Plant Cell 1, no. 12 (1989): 1147–56. http://dx.doi.org/10.1105/tpc.1.12.1147.

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Huang, Xiao-Fang, Binh Nguyen-Quoc, and Serge Yelle. "MOLECULAR CHARACTERIZATION AND TRANSIENT EXPRESSION OF MAIZE SUS1 PROMOTER." HortScience 29, no. 4 (1994): 253f—254. http://dx.doi.org/10.21273/hortsci.29.4.253f.

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Sucrose synthase (SS) is one of the key enzymes in plant carbohydrate metabolism. In maize, this enzyme is encoded by two genes, Sh1 and Sus1. We have isolated and determined the 5'-upstream sequence of maize Sus1 gene and compared it with the corresponding sequence in Sh1 gene. Sequence analysis revealed that there was a weak homology between the two promoters and no common sequence elements were found. To understand the differential regulation of the expression of the two genes, we constructed chimeric GUS fusions using the two promoters of SS genes. By using the biolistic system, we deliver

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Auriac, Marie-Christine, та Antonius C. J. Timmers. "Nodulation Studies in the Model Legume Medicago truncatula: Advantages of Using the Constitutive EF1α Promoter and Limitations in Detecting Fluorescent Reporter Proteins in Nodule Tissues". Molecular Plant-Microbe Interactions® 20, № 9 (2007): 1040–47. http://dx.doi.org/10.1094/mpmi-20-9-1040.

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The Cauliflower mosaic virus 35S promoter currently is being used in RNAi-based approaches for attenuating host gene expression during legume root nodule development and also for the expression of fluorescent reporters in nodule tissues. In this study, we have evaluated the expression of this promoter in the indeterminate nodules of the model plant Medicago truncatula. Our results clearly show that the 35S promoter is inactive in both the nodule meristem and in bacteroid-containing cells of the nodules. On the other hand, the Arabidopsis thaliana EF1α promoter was found to be strongly expresse

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Chu, Ling-Hui, and Shih-Tong Jeng. "Multiple transduction pathways regulate the 35S promoter with an ABA responsive element." Plant Science 163, no. 1 (2002): 23–32. http://dx.doi.org/10.1016/s0168-9452(02)00055-9.

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Benfey, P. N., and N. H. Chua. "The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants." Science 250, no. 4983 (1990): 959–66. http://dx.doi.org/10.1126/science.250.4983.959.

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PAPARINI, ANDREA, and VINCENZO ROMANO-SPICA. "Gene Transfer and Cauliflower Mosaic Virus Promoter 35S Activity in Mammalian Cells." Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes 41, no. 4 (2006): 437–49. http://dx.doi.org/10.1080/03601230600616957.

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Schnurr, J. A., and D. J. Guerra. "The CaMV-35S promoter is sensitive to shortened photoperiod in transgenic tobacco." Plant Cell Reports 19, no. 3 (2000): 279–82. http://dx.doi.org/10.1007/s002990050012.

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Gatz, Christiane, Jens Katzek, Salome Prat, and Arnd Heyer. "Repression of the CaMV 35S promoter by the octopine synthase enhancer element." FEBS Letters 293, no. 1-2 (1991): 175–78. http://dx.doi.org/10.1016/0014-5793(91)81180-g.

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Rajeswaran, Rajendran, Sukumaran Sunitha, Padubidri V. Shivaprasad, Mikhail M. Pooggin, Thomas Hohn, and Karuppannan Veluthambi. "The Mungbean Yellow Mosaic Begomovirus Transcriptional Activator Protein Transactivates the Viral Promoter-Driven Transgene and Causes Toxicity in Transgenic Tobacco Plants." Molecular Plant-Microbe Interactions® 20, no. 12 (2007): 1545–54. http://dx.doi.org/10.1094/mpmi-20-12-1545.

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The Begomovirus transcriptional activator protein (TrAP/AC2/C2) is a multifunctional protein which activates the viral late gene promoters, suppresses gene silencing, and determines pathogenicity. To study TrAP-mediated transactivation of a stably integrated gene, we generated transgenic tobacco plants with a Mungbean yellow mosaic virus (MYMV) AV1 late gene promoter-driven reporter gene and supertransformed them with the MYMV TrAP gene driven by a strong 35S promoter. We obtained a single supertransformed plant with an intact 35S-TrAP gene that activated the reporter gene 2.5-fold. However, 1

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White, Elizabeth A., Charles L. Clark, Veronica Sanchez, and Deborah H. Spector. "Small Internal Deletions in the Human Cytomegalovirus IE2 Gene Result in Nonviable Recombinant Viruses with Differential Defects in Viral Gene Expression." Journal of Virology 78, no. 4 (2004): 1817–30. http://dx.doi.org/10.1128/jvi.78.4.1817-1830.2004.

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ABSTRACT The human cytomegalovirus (HCMV) IE2 86-kDa protein is a key viral transactivator and an important regulator of HCMV infections. We used the HCMV genome cloned as a bacterial artificial chromosome (BAC) to construct four HCMV mutants with disruptions in regions of IE2 86 that are predicted to be important for its transactivation and autoregulatory functions. Three of these mutants have mutations that remove amino acids 356 to 359, 427 to 435, and 505 to 511, which disrupts a region of IE2 86 implicated in the activation of HCMV early promoters, a predicted zinc finger domain, and a pu

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Arce-Johnson, Patricio, Ulrich Reimann-Philipp, Hal S. Padgett, Rafael Rivera-Bustamante, and Roger N. Beachy. "Requirement of the Movement Protein for Long Distance Spread of Tobacco Mosaic Virus in Grafted Plants." Molecular Plant-Microbe Interactions® 10, no. 6 (1997): 691–99. http://dx.doi.org/10.1094/mpmi.1997.10.6.691.

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Systemic spread of tobacco mosaic virus (TMV) that lacks a functional movement protein (TMVΔMP) was investigated in grafted tobacco (Nicotiana tabacum) plants. Transgenic plants that express the 30-kDa movement protein (MP) gene (MP) under the control of the rolC (phloem-specific) or pal2 (xylem-specific) promoters were unable to support systemic infection by the mutant virus, while plants that express the MP gene from the cauliflower mosaic virus 35S promoter (35S:MP) led to systemic infection. Doubly grafted plants were constructed in which plants containing the 35S:MP gene were used as root

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Takeda, Naoya, Kristina Haage, Shusei Sato, Satoshi Tabata, and Martin Parniske. "Activation of a Lotus japonicus Subtilase Gene During Arbuscular Mycorrhiza Is Dependent on the Common Symbiosis Genes and Two cis-Active Promoter Regions." Molecular Plant-Microbe Interactions® 24, no. 6 (2011): 662–70. http://dx.doi.org/10.1094/mpmi-09-10-0220.

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The subtilisin-like serine protease SbtM1 is strongly and specifically induced during arbuscular mycorrhiza (AM) symbiosis in Lotus japonicus. Another subtilase gene, SbtS, is induced during early stages of nodulation and AM. Transcript profiling in plant symbiosis mutants revealed that the AM-induced expression of SbtM1 and the gene family members SbtM3 and SbtM4 is dependent on the common symbiosis pathway, whereas an independent pathway contributes to the activation of SbtS. We used the specific spatial expression patterns of SbtM1 promoter β-d-glucuronidase (GUS) fusions to isolate cis ele

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