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Dissertations / Theses on the topic 'Protein display'

Author: Grafiati
Published: 28 May 2022
Last updated: 30 July 2025

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1

Nilsson, Mikael. "Protein-DNA recognition : in vitro evolution and characterization of DNA-binding proteins /." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4269.

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2

Löfblom, John. "Staphylococcal surface display for protein engineering and characterization." Doctoral thesis, KTH, Skolan för bioteknologi (BIO), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

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Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. Directed evolution has provided researchers with a tool for circumventing our limited knowledge and hence the possibility to create novel molecules that by no means could have been designed today. The approach is based on construction of high-complexity combinatorial librarie
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Löfblom, John. "Staphylococcal surface display for protein engineering and characterization /." Stockholm : Bioteknologi, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4584.

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4

Wheeler, Lucas. "The Evolution of Metal and Peptide Binding in the S100 Protein Family." Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23178.

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Proteins perform an incredible array of functions facilitated by a diverse set of biochemical properties. Changing these properties is an essential molecular mechanism of evolutionary change, with major questions in protein evolution surrounding this topic. How do new functional biochemical features evolve? How do proteins change following gene duplication events? I used the S100 protein family as a model to probe these aspects of protein evolution. The S100s are signaling proteins that play a diverse range of biological roles binding Calcium ions, transition metal ions, and other prote
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5

Mizrachi, Eshchar. "Solubility, particle formation and immune display of trimers of major capsid protein 7 of African horsesickness virus fused with enhanced green fluorescent protein." Diss., Pretoria : [s.n.], 2009. http://upetd.up.ac.za/thesis/available/etd-06082009-161825.

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6

Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/30392/1/Andrew_Pow_Thesis.pdf.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precurso
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7

Pow, Andrew James. "Protein complementation assay as a display system for screening protein libraries in the intracellular environment." Queensland University of Technology, 2008. http://eprints.qut.edu.au/30392/.

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A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precurso
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8

Gragg, Megan Ellen. "Mutant Rhodopsins in Autosomal Dominant Retinitis Pigmentosa Display Variable Aggregation Properties." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1522935340252319.

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9

Kronqvist, Nina. "Staphylococcal surface display in directed evolution." Doctoral thesis, KTH, Molekylär Bioteknologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-11555.

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Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. In this approach, generally termed directedevolution, high-complexity combinatorial libraries are created from which affinity proteins areisolated using an appropriate selection method, thus cir
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10

Meyer, Scott C. "Non-Covalent Selection Methodologies Utilizing Phage Display." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/194059.

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In nature, non-covalent interactions are as important and dynamic as they are elusive. As such, the study of non-covalent interactions both in vivo and in vitro has proven to be challenging. Given the potential benefits of elucidating protein-protein, ligand-receptor, and other biologically relevant interactions, the development of methodologies for the study of non-covalent interactions is an attractive goal.Biologically encoded protein and peptide libraries that connect the genotypic information with the expressed phenotype have emerged in recent years as powerful methods for studying non
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11

Fan, Yan Baranger Anne M. Katzenellenbogen John A. Zhao Huimin Silverman Scott K. "Exploring protein-RNA interactions with site-directed mutagenesis and phage display." Urbana, IL.: University of Illinois, 2009. http://hdl.handle.net/2142/14755.

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12

Camacho-Soto, Karla. "Selective Control of Protein Kinases and Phosphatases." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/578903.

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The reversible phosphorylation of proteins plays a key role in nearly every aspect of cell life. This essential post-translational modification controls a myriad of cellular events from cell survival, differentiation, and migration to apoptosis. Two classes of enzymes, kinases and phosphatases, tightly control all phosphorylation events. Perturbation in the activity of any member of these classes of enzymes has been linked to numerous diseases including cancer, metabolic disorders, immune disorders and neurological disorders. Therefore, there is a great interest among the scientific community
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13

Fleetwood, Filippa. "Bacterial display systems for engineering of affinity proteins." Doctoral thesis, KTH, Proteinteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-156420.

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Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. An attractive technology for this purpose is cell surface display, offering many advantages, such as the quantitative isolation of high-affinity library members using flow-cytometric cell sorting. This thesis describes the development, evaluation and use of bacterial display technologies for the engineering of affinity proteins. Affinity proteins
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14

Palà, Pujadas Judith. "Synthesis of Sonic Hedgehog protein and development of peptide binders using phage display libraries." Doctoral thesis, Universitat de Barcelona, 2018. http://hdl.handle.net/10803/552403.

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Chemical protein synthesis has become a potent tool for the synthesis of proteins, especially those which are not easily accessed by DNA recombinant methods, such as proteins with posttranslational modifications. The key reaction in chemical protein synthesis is Native Chemical Ligation, which implies a chemoselective ligation between two unprotected peptide fragments in aqueous solution. It requires a cysteine residue at the N-terminal of one peptide, and a thioester moiety at the C-terminal of the other fragment. Several strategies have been developed for the synthesis of C-terminal thioeste
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15

McLean, Hector Alexander. "Application of phage display to the study of toxin-receptor interactions." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301779.

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16

Grouzdev, D. S., M. V. Dziuba, D. V. Kurek, and A. I. Ovchinnikov. "The Method for Protein Display on the Surface of Bacterial Magnetic Nanoparticles." Thesis, Sumy State University, 2013. http://essuir.sumdu.edu.ua/handle/123456789/35488.

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In this study, we present a comprehensive approach to the design and development of magnetosomes with antibodies immobilized on their surface by integration in membrane in vitro. Designed fusion proteins Mbb and Mistbb consisted of anchor proteins and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used for development of IgG-binding magnetosomes. The magnetosome membrane protein MamC and membrane protein of Bacillus subtilis Mistic were selected as anchor proteins. Using Response Surface Methodology (RSM), the high level of fusion proteins integration into bacterial n
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17

Niemand, Jandeli. "A phage display study of interacting peptide binding partners of malarial S-Adenosylmethionine decarboxylase/Ornithine decarboxylase." Diss., University of Pretoria, 2007. http://hdl.handle.net/2263/24105.

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Due to the increasing resistance against the currently used antimalarial drugs, novel chemotherapeutic agents that target new metabolic pathways for the treatment of malarial infections are urgently needed. One approach to the drug discovery process is to use interaction analysis to find proteins that are involved in a specific metabolic pathway that has been identified as a drug target. Protein-protein interactions in such a pathway can be preferential targets since a) there is often greater structural variability in protein-protein interfaces, which can lead to more effective differentiation
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18

Lamba, Vandana. "Development of Potent and Selective Bivalent Inhibitors for Protein Kinases Utilizing Phage Display." Diss., The University of Arizona, 2012. http://hdl.handle.net/10150/265559.

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Protein kinases function as key regulators in a variety of signaling pathways by executing the phosphorylation of a variety of protein substrates. Perturbation in the activity of numerous proteins kinases has been implicated in a large number of diseases including cancer, diabetes, inflammation and neurological disorders. Therefore, selective modulation of kinase activity is highly desirable for the dissection of complex signaling pathways and substantiating therapeutic targets. To develop potent and selective inhibitors for an array of kinases, our group has developed a fragment based bivalen
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19

George, Miriam Thankam. "Immunological Characterization Of Duffy Binding Protein Of Plasmodium vivax." Scholar Commons, 2015. http://scholarcommons.usf.edu/etd/5689.

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Plasmodium vivax Duffy binding protein (DBP) is an essential ligand for reticulocyte invasion making it a premier asexual blood stage vaccine candidate. However, strain-specific immunity due to DBPII allelic variation may complicate vaccine efficacy, suggesting that an effective DBPII vaccine needs to target immune responses to conserved epitopes that are potential targets of strain-transcending neutralizing immunity. Anti DBPII monoclonal antibodies, which were previously characterized by COS7 cell binding assay as inhibitory and non-inhibitory to DBPII-erythrocyte binding, were mapped to DBP
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20

Alm, Tove. "Interaction engineered three-helix bundle domains for protein recovery and detection." Doctoral thesis, KTH, Proteomik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-12823.

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HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. Traditionally, monoclonal antibodies have been the natural choice for biotechnological and therapeutic applications due to their ability to bind a huge range of different molecules and their natural good affinity. However, the large size of antibodies (15
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21

Åstrand, Mikael. "Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications." Doctoral thesis, KTH, Proteinteknologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-186279.

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Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. In this thesis, the albumin-binding domain is explored as a scaffold for engineering novel affinity proteins with the possible benefit of combining a prolonged serum half-life with specific targeting in a single small scaffold protein. Previously, a library was created by randomizing surf
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22

Stein, Viktor. "A protein display system based on human O⁶-alkylguanine alkyltransferase and in vitro compartmentalisation." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611232.

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23

Ramkissoon, Yashin Danjay. "Interaction cloning by phage display : protein interactions of the human testis determining factor, SRY." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627297.

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24

Filipovic, Nedim. "Phage display to identify functional resistance mutations to Rigosertib." Scholarship @ Claremont, 2017. http://scholarship.claremont.edu/cmc_theses/1475.

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In vitro protein selection has had major impacts in the field of protein engineering. Traditional screens assay individual proteins for specific function. Selection, however, analyzes a pool of mutants and yields the best variants. Phage display, a successful selection technique, also provides a reliable link between variant phenotype and genotype. It can also be coupled with high throughput sequencing to map protein mutations; potentially highlighting vital mutations in variants. We propose to apply this technique to cancer therapy. RAF, a serine/threonine kinase, is critical for cell regulat
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25

Lehtiö, Janne. "Functional studies and engineering of family 1 carbohydrate-binding modules." Doctoral thesis, KTH, Biotechnology, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3211.

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<p>The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives.</p><p>The characteristics and specificity of CBM1s from the<i>Trichoderma reesei</i>Cel7A and Cel6A, along with severalother wild type and mutated CBMs, were studied using bindingexperiments and transmission electron microscopy (TEM). Datafrom the binding studies confirmed that the presence of onetrypto
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26

Shomin, Carolyn. "A BIVALENT METHODOLOGY FOR TARGETING PROTEIN KINASES: CONJUGATING PHAGE DISPLAY SELECTED CYCLIC PEPTIDES TO STAUROSPORINE." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202950.

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Protein kinases constitute essential biological and target class owing to the vital function of reversible phosphorylation catalyzed by these enzymes. With more than 500 kinases in the human genome, containing conserved structure and overlapping function, pose challenging targets for inhibition. Alternative methods for targeting protein kinases remain warranted as the traditional methods are biased toward ATP-competitive compounds. These methods have yielded successful therapeutics, however toxicity due to nonselectivity and limited development potential due to intense drug discovery effort
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27

Sheth, Vrunda. "Visualization of protein 3D structures in reduced represetnation with simultaneous display of intra and inter-molecular interactions /." Online version of thesis, 2009. http://hdl.handle.net/1850/10857.

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28

Hjelm, Barbara. "Epitope mapping of antibodies towards human protein targets." Doctoral thesis, KTH, Proteomik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-59529.

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This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. Techniques for epitope mapping in a streamlined manner are therefore needed, particularly in high throughput projects as the Human Protein Atlas that aims to systematically generate two antibodies with se
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Al, Qaraghuli Mohammed. "Investigating the antibody recognition of different hapten classes using a combination of phage display and protein modelling." Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=214816.

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30

Wikman, Maria. "Rational and combinatorial protein engineering for vaccine delivery and drug targeting." Doctoral thesis, Stockholm : Department of Biotechnology, Royal Insitute of Technology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-231.

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31

Grimm, Sebastian. "Ribosome display for selection and evolution of affibody molecules." Doctoral thesis, KTH, Molekylär Bioteknologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-33191.

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Affinity proteins are invaluable tools in biotechnological and medical applications. This thesis is about combinatorial protein engineering principles for the generation of novel affinity proteins to purify mouse immunoglobulin, detect a potential cancer marker protein or inhibit a cell proliferation pathway. In a first study, ribosome display was for the first time applied to the selection of so-called affibody molecules, including the design of a ribosome display gene cassette, initial test enrichment experiments and the selection of binders against murine IgG1. One of the selected binders (
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32

Nilvebrant, Johan. "An albumin-binding domain as a scaffold for bispecific affinity proteins." Doctoral thesis, KTH, Proteomik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-105425.

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Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. By means of combinatorial protein engineering and protein library technology, smaller antibody fragments or alternative non-immunoglobulin protein scaffolds can be engineered for various functions based on molecular recognition. In this thesis, a 46 amino acid small albumin-binding domain derived from streptococcal protein G was evaluated as a scaffold fo
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33

Osei, Awuku Kwabena. "Superantigen-like interaction of IVIG with antibody Fab fragments cloned by phage display technology." Doctoral thesis, [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=96462785X.

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34

Eklund, Malin. "Combinatorial protein engineering applied to enzyme catalysis and molecular recognition." Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3721.

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<p>The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. In one section of thework underlying this thesis, one such technique (phagedisplay), was used to isolateand identify protein librarymembers based on their catalytic or target molecule-bindingproperties.</p><p>In a fir
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35

Ding, Daniel N. "Membrane display of a fusion protein containing the ice-nucleation protein from Pseudomonas syringae and ScFv against c-myc oncoprotein in recombinant Escherichia coli." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0001/MQ42060.pdf.

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36

Sulic, Ana-marija. "Identification of tissue transglutaminase protein network." Doctoral thesis, Università degli studi di Trieste, 2011. http://hdl.handle.net/10077/4484.

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2009/2010<br>Tissue transglutaminase (TG2) is a multifunctional enyzme involved in cell growth and differentiantion, receptor mediated endocytosis, cell adhesion and morphology, stabilization of extracellular matrix, membrane trafficking and structure/function, signal transduction, regulation of cytoskeleton and apoptosis. Multiple lines of evidence suggest an involvement of TG2 autoimmune diseases, cancer and in neurodegenerative diseases, including Alzheimer's disease, progressive supranuclear palsy, Huntington's disease and Parkinson's disease. In all of the neurodegenerative diseases exa
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37

Gilbert, Michele. "Design of synthetic peptides that display cell binding and signaling sequences on calcium phosphate surfaces /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/8063.

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38

Ek, Moira. "Bacterial Display of a Tau-Binding Affibody Construct:Towards Affinity Maturation." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278580.

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Aggregation of microtubule-associated protein tau is involved in the pathology of several neurodegenerative diseases, including Alzheimer’s disease. The affibody TP4 has been shown to inhibit this aggregation process, and its target-binding positions were previously grafted onto a dimeric affibody scaffold, creating the sequestrin seqTP4. This project constitutes a part of the affinity maturation process of seqTP4, using two different bacterial display methods. An error-prone PCR library was first expressed on Staphylococcus carnosus cells for selection of variants with improved tau-binding pr
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39

Suharni. "Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor." Kyoto University, 2015. http://hdl.handle.net/2433/195961.

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40

TARASIUK, OLGA. "Phage display selection and development of monoclonal antibodies against Protein Disulfide Isomerase, a novel tumor associated antigen and potential target for cancer immunotherapy." Doctoral thesis, Università del Piemonte Orientale, 2018. http://hdl.handle.net/11579/105204.

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41

Crépin, Ronan. "Génération d'anticorps thérapeutiques par phage display dirigés contre le récepteur de la transferrine et le récepteur Kit." Paris 11, 2010. http://www.theses.fr/2010PA11T081.

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42

Rimbault, Charlotte. "Modulation des interactions impliquant les domaines PDZ par une approche d’évolution dirigée." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0438/document.

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Les interactions protéine-protéine (IPPs), complexes et dynamiques, sont le cœur des réseaux protéiques cellulaires. Au niveau des synapses excitatrices, la densité post-synaptique (PSD) est un exemple typique de réseau protéique dont la structure et la composition à l’échelle nanoscopique détermine la fonction cellulaire. Ainsi, la régulation dynamique de la composition de la PSD et des mouvements des récepteurs au glutamate dans ou hors de la PSD constitue la base des théories moléculaires actuelles sur l’apprentissage et la mémoire. Dans ce contexte, durant ma thèse, j’ai étudié une classe
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43

ZIPPILLI, Lorenza. "Epitope mapping of a mAb against the factor H binding protein (fHbp) of N. meningitidis by phage display." Doctoral thesis, Università degli studi del Molise, 2012. http://hdl.handle.net/11695/66305.

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Attualmente non è ancora disponibile alcun vaccino ampiamente totalmente efficace per la prevenzione della meningite di gruppo B. Uno dei più promettenti candidati per lo sviluppo di un vaccino è la proteina che lega il fattore H (factor H-binding protein o fHbp), una lipoproteina di superficie espressa in tutti i ceppi di Neisseria meningitidis. La sua funzione è quella di legare il fattore H (fH), una molecola chiave della via alternativa della cascata del complemento. Il patogeno, legando sulla sua superficie il fH, riesce a bloccare l'attivazione del complemento e ad eludere le difese immu
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Kurup, Sindhulakshmi. "Design of Oligosaccharide Libraries to Characterize Heparan Sulfate – Protein Interactions." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7095.

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45

Igloi, Zsofia. "Identifying novel interaction partners for the hepatitis C virus NS5A protein by screening a human SH3 domain phage display library." Thesis, University of Leeds, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574514.

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The hepatitis C virus is a worldwide cause of liver disease by infecting approximately 2 % of the population. One of its non-structural protein, NS5A, Is known to interact with many cellular proteins involved in cell signaling pathways. Doing so modifies the outcome of the signalling for the benefit of virus replication and survival. We have previously demonstrated that NSSA contains two Class II poly-proline motifs in the second low complexity sequence, with the consensus sequence Pro-X-X-Pro-X-Lys/ Arg, which mediate the interaction with SH3 domain containing proteins. Substitution of the pr
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Vasilca, Vlad. "Nanoscale spherical-supported membranes as novel platforms for improving the phage display screening of antibody mimetics against membrane protein targets." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/16301/.

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Membrane proteins represent the majority of therapeutic targets for the antibody-based drugs available today. These are routinely identified via phage display screening, but traditional antigen presentation methods require membrane protein targets to be detergent-solubilised in order to preserve their native conformations post-purification. Unfortunately, detergent solubilisation can not only lead to gradual target denaturation over time, but the detergent micelles can also occlude important epitopes on the extramembranous loops and thus prevent the discovery of antibody binders. The current t
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47

Andersson, Ken G. "Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging." Doctoral thesis, KTH, Proteinteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-213451.

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Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. Further, the thesis describes rational design of Affibody-based tracers, intended for future patient stratification using medical imaging. Fusing recombinant proteins to various autotransporters is a promising approach for efficient surface display on the surface of E. coli, as well as for construction of high-complexity libraries. In paper I, we successfully engineered an
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48

Watkinson, Jonathan I. "Characterization of two genes, trehalose-6-phosphate synthase/phosphatase and nucleotide binding protein, shown to be differentially regulated in roots of Cypripedium parviflorum var. pubescens grown with a mycorrhizal fungus Thanatephorus pennatus." Diss., Virginia Tech, 2002. http://hdl.handle.net/10919/27467.

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The analysis of gene changes associated with formation of the mycorrhizal symbiosis between orchid and fungi could have broad implications for plant pathogen interactions. Fungi associated with North American terrestrial orchids were once included in the pathogenic genus Rhizoctonia. This suggests that orchids are able to overcome or utilize normally pathogenic pathways to establish symbioses. A differential display technique was employed to analyze gene changes in orchid in response to a fungus. Samples of RNA from roots of Cypripedium parviflorum var. pubescens (CyPP) grown in the presenc
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Pruvost, Tiphanie. "Ingénierie moléculaire de la réactivité croisée inter-espèces d’anticorps thérapeutiques par Yeast Surface Display." Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPASQ074.

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Le succès des anticorps monoclonaux comme outils thérapeutiques s'explique en partie par leur grande spécificité pour leur cible. Celle-ci implique souvent qu'un anticorps développé contre une cible humaine échoue à la reconnaître chez les espèces utilisées lors des essais précliniques. L'objectif de ces travaux est de développer une méthode d'ingénierie de protéine permettant à un anticorps de reconnaître un antigène provenant d'espèces différentes en utilisant comme modèles deux anticorps reconnaissant la protéine LAMP1 humaine mais pas ses versions murine et simienne. Durant ce projet le pr
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Pham, Errek Manh Trung. "Producing A Peptide For Use In A Blood Biosensor For Injury Detection." Youngstown State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1607519672342672.

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