Relevant bibliographies by topics / Protein Dynamics - Confocal Microscopy / Dissertations / Theses
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Dissertations / Theses on the topic 'Protein Dynamics - Confocal Microscopy'

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Published: 7 September 2023

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1

Nilufar, Rahimova. "Real-time dynamics of IκBαdegradation studied with Kusabira-Orange 2 fusion proteins". 京都大学 (Kyoto University), 2016. http://hdl.handle.net/2433/217147.

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2

Gösch, Michael. "Microfluidic analysis and parallel confocal detection of single molecules /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-663-4/.

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3

Piguet, Joachim. "Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics." Doctoral thesis, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-178147.

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Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. A receptor associated protein, rapsyn, acts as a linker between the receptor and the other components of the synaptic suramolecular assembly. Advances in fluorescence microscopy have allowed to measure the behavior of a single receptor in the cell membrane. In this work single-
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4

Elmlund, Hans. "Protein structure dynamics and interplay : by single-particle electron microscopy." Doctoral thesis, Stockholm : Teknik och hälsa, Technology and Health, Kungliga Tekniska högskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4669.

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5

Guo, Qing. "Single Molecule Optical Magnetic Tweezers Microscopy Studies of Protein Dynamics." Bowling Green State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1435334948.

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6

SCIPIONI, LORENZO. "Local image correlation methods for the characterization of subcellular structure and dynamics by confocal and super-resolution microscopy." Doctoral thesis, Università degli studi di Genova, 2018. http://hdl.handle.net/11567/929279.

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This thesis work aspires to present a new concept for the application of correlation techniques to the study of the cellular environment. By exploiting local analysis in combination to a fast fit-free technique (the phasor approach) we provide an exhaustive high-resolution analysis of structural and dynamic properties while maintaining a reasonable computation time. The dissertation will be articulated as follows: In CHAPTER 1 we aim to provide the reader with a description of the techniques that will be exploited during the rest of the dissertation together with the open questions and proble
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7

Vallejo, Rodriguez Johana. "Compartmentation of glycolysis to a plasma membrane domain : role of caveolin-1 as a scaffolding protein for phosphofructokinase /." Free to MU Campus, others may purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3137759.

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8

Ljunglöf, Anders. "Direct observation of biomolecule adsorption and spatial distribution of functional groups in chromatographic adsorbent particles." Doctoral thesis, Uppsala University, Surface Biotechnology, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1602.

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<p>Confocal microscopy has been used as a tool for studying adsorption of biomolecules to individual chromatographic adsorbent particles. By coupling a fluorescent dye to protein molecules, their penetration into single adsorbent particles could be observed visually at different times during batch uptake. By relating the relative fluorescence intensity obtained at different times to the value at equilibrium, the degree of saturation versus time could be constructed. The use of two different fluorescent dyes for protein labeling and two independent detectors, allowed direct observation of a two
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9

des, Georges Amédée. "Regulation of tubulin dynamics by the +Tip tracking protein Mal3." Thesis, University of Cambridge, 2008. https://www.repository.cam.ac.uk/handle/1810/256531.

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The Microtubule (MT) network is a central component of the eukaryotic cell cytoskeleton. In the fission yeast S. pombe, a complex of three proteins specifically tracks MT +ends and stabilizes MTs in the cell. It is composed of the proteins Mal3, Tip1 and Tea2. Mal3, the S. pombe homologue of EB1, is a highly conserved ubiquitous protein found to be at the centre of many MT related processes. Tip1 is a CLIP170 homologue and Tea2 a kinesin-like motor protein. The mechanism by which they target the growing end of MTs and stabilize them is still unknown. A combination of biochemistry, electron mic
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10

Roy, Chowdhury Susovan. "Single-Molecule Force Manipulation and Nanoscopic Imaging of Protein Structure-Dynamics-Function Relationship." Bowling Green State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu162707900722617.

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11

Huber, Scott David. "On Protein Recruitment Dynamics in Clathrin-Mediated Endocytosis and its Relation to Membrane Tension." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1545921340769193.

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12

Torella, Joseph Peter. "Confocal single-molecule fluorescence as a tool for investigating biomolecular dynamics in vitro and in vivo." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:f57d1984-8db9-4d79-b333-f1be507ca3bf.

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Confocal single-molecule fluorescence is a powerful tool for monitoring conformational dynamics, and has provided new insight into the enzymatic activities of complex biological molecules such as DNA and RNA polymerases. Though useful, such studies are typically qualitative in nature, and performed almost exclusively in highly purified, in vitro settings. In this work, I focus on improving the methodology of confocal single-molecule fluorescence in two broad ways: (i) by enabling the quantitative identification of molecular dynamics in proteins and nucleic acids in vitro, and (ii) developing t
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13

Sadler, Emma Elizabeth. "Single-molecule fluorescence studies of KirBac1.1." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:214fcd74-7384-4ade-ac17-7cac5c44a05c.

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Inwardly rectifying potassium (Kir) channels are essential for controlling the excitability of eukaryotic cells, forming a key part of the inter-cellular signalling system in multi-cellular organisms. However, as prokaryotic (KirBac) channels are less technically challenging to study in vitro and have been shown to be directly homologous to eukaryotic channels, they are often studied in lieu of their mammalian counterparts. A vital feature of Kir and KirBac channels is their mechanism for opening and closing, or their gating: this study predominantly features observations of open and/or closed
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14

Cao, Jin. "Single Molecular Spectroscopy and Atomic Force Manipulation of Protein Conformation and Dynamics." Bowling Green State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1416588612.

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15

KATIYAR, AMIT. "Development of Novel Mesoporous Silicates for Bioseparations and Biocatalysis." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1204765205.

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16

Wang, Dan. "Profiling Cell Surface Sialylation and Desialylation Dynamics of Immune Cells." Cleveland State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1468086662.

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17

Landgraf, Dirk. "Quantifying Localizations and Dynamics in Single Bacterial Cells." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10612.

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Levels of macromolecules fluctuate both spatially and temporally in individual cells. Such heterogeneity could be exploited for bet hedging in uncertain environments, or be suppressed by negative feedback if perturbations are deleterious. For the master stress-response regulator in Escherichia coli, RpoS, both of these scenarios have been suggested. RpoS levels are also exceedingly low and controlled by the ClpXP protease, which reportedly displays extreme spatial heterogeneity. However, little is known quantitatively about RpoS dynamics. This is partly because no functional protein fusions ex
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18

Stadler, Charlotte. "Towards subcellular localization of the human proteome using bioimaging." Doctoral thesis, KTH, Proteomik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-103616.

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Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. The objective of the studies presented in this doctoral thesis was to systematically explore the human proteome at the subcellular level using bioimaging and to develop techniques for validation of
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19

Jablonski, Amy E. "Optically modulated fluorescent proteins." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/52327.

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Optical modulation has shown the selective and sensitive signal improvement in high background systems in cell imaging; however, cell applications are still limited due to biocompatibility and delivery issues. Fluorescent proteins have a variety of optically accessible states that make them ideal candidates for investigation of modulatability. Combining the optical modulation technique with the biocompatibility of fluorescent proteins is a major advance. This work focuses on evaluation fluorescent proteins and their optical states for modulation, as well demonstrations of cellular imaging.
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20

Lundberg, Emma. "Bioimaging for analysis of protein expression in cells and tissues using affinity reagents." Doctoral thesis, Stockholm : School of biotechnology, Royal institute of technology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4862.

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21

Dhakal, Santosh. "Impact of High Pressure Processing on Immunoreactivity and SomePhysico-chemical Properties of Almond Milk." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1374017194.

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22

Dhital, Bharat. "Single-molecule interfacial electron transfer dynamics in solar energy conversion." Bowling Green State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1477997482545831.

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23

Vogel, Alexander, Jörg Nikolaus, Katrin Weise, et al. "Interaction of the human N-Ras protein with lipid raft model membranes of varying degrees of complexity." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-191006.

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Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), and unsaturated phospholipids show stable phase separation and are often used as model systems of lipid rafts. Yet, their ability to reproduce raft properties and function is still debated. We investigated the properties and functional aspects of three lipid raft model systems of varying degrees of biological relevance – PSM/POPC/Chol, DPPC/POPC/Chol, and DPPC/DOPC/Chol – using 2H solidstate nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, and atomic force microscopy. While
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24

Coffin, Jared M. "THE ROLE OF PROTEIN AS A FOAM BOOSTER IN THE PRESENCE OF OIL." Miami University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1564440064244048.

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25

Chen, Huiyi. "System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10044.

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Gene expression is a fundamental process in the cell and is made up of two parts – the information flow from DNA to RNA, and from RNA to protein. Here, we examined specific sub-processes in Escherichia coli gene expression using newly available tools that permit genome-wide analysis. We begin our studies measuring mRNA and protein abundances in single cells by single-molecule fluorescence microscopy, and then focus our attention to studying RNA generation and degradation by high throughput sequencing. The details of the dynamics of gene expression can be observed from fluctuations in mRNA and
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26

Derelli, Davide. "Self-assembly of colloidal particles - a combination of experimental and simulation approaches." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2019. http://amslaurea.unibo.it/18076/.

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In this project, self-assembly behaviour of colloidal particles was investigated by a combination of both computer simulations and experimental approach. In particular, a Brownian Dynamics algorithm was used to simulate either steep-repulsive spheres or spherocylinders in a shrinking spherical confinement. In accordance with literature, in the former case packed spheres were shown to crystallize into a distinctive icosahedral structure. In the latter study, spherocylinders clearly revealed a local tendency to form smectic layers. After the synthesis of micro-sized fluorescent-labelled silica s
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27

Rahimi, Khameneh Shabnam. "Assessment of Retroviruses as Potential Vectors for the Cell Delivery of Prions." Thèse, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23472.

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Transmissible spongiform encephalopathies (TSEs) or prion diseases are a class of fatal brain disorders better known as Creutzfeldt-Jacob Disease (CJD) in humans, bovine spongiform encephalopathy (BSE) in cattle, scrapie in sheep, and chronic wasting disease (CWD) in deer and elk. The infectious agent responsible for these diseases is a misfolded prion protein capable of catalyzing a conformational change in normal cellular prion proteins (PrPC) into aberrant disease-causing structural isoforms (PrPSc). Although the etiological agent for TSEs has clearly been defined as PrPSc, there are import
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28

Vogel, Alexander, Jörg Nikolaus, Katrin Weise, et al. "Interaction of the human N-Ras protein with lipid raft model membranes of varying degrees of complexity." de Gruyter, 2014. https://ul.qucosa.de/id/qucosa%3A14050.

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Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), and unsaturated phospholipids show stable phase separation and are often used as model systems of lipid rafts. Yet, their ability to reproduce raft properties and function is still debated. We investigated the properties and functional aspects of three lipid raft model systems of varying degrees of biological relevance – PSM/POPC/Chol, DPPC/POPC/Chol, and DPPC/DOPC/Chol – using 2H solidstate nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, and atomic force microscopy. While
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29

Knowles-Barley, Seymour Francis. "Proteins, anatomy and networks of the fruit fly brain." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/6177.

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Our understanding of the complexity of the brain is limited by the data we can collect and analyze. Because of experimental limitations and a desire for greater detail, most investigations focus on just one aspect of the brain. For example, brain function can be studied at many levels of abstraction including, but not limited to, gene expression, protein interactions, anatomical regions, neuronal connectivity, synaptic plasticity, and the electrical activity of neurons. By focusing on each of these levels, neuroscience has built up a detailed picture of how the brain works, but each level is u
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30

Padilla, Roberto. "Discovering the Potential of Photoluminescent Ruthenium(II) Complexes as Photodynamic Therapy Agents." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/78190.

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Anthracene was attached to light activated, ruthenium-based DNA disruptors to probe their distribution in cancer cells. The objective of this research is to understand the photophysical properties (Chapter 2), photoreactivity toward DNA and proteins (Chapter 3), and localization within cancer cells (Chapter 4) of ruthenium complexes that demonstrate promise as photodynamic therapy (PDT) agents. [(AnthbpyMe)(bpy)Ru(dpp)]2+ (1) and [(AnthbpyMe)2Ru(dpp)]2+ (2) absorb visible light with metal-to-ligand charge transfer (MLCT) transitions at 459 nm (16,000 M-1 cm-1 ) and 461 nm (21,000 M-1 cm-1 ),
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31

Klajner, Piotr. "Experimental study of the kinetics of two systems : DNA complexation by the NCp7 protein and probe dynamics in a glassy colloidal suspension." Phd thesis, Université de Strasbourg, 2012. http://tel.archives-ouvertes.fr/tel-00858886.

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In the first part of this thesis, we study the kinetics of the complexation of a double-stranded DNA byNCp7 protein. To do this, we study the evolution of mechanical properties of DNA and its complexation by stretching the DNA/NCp7 complex with a optical trap. We observed that the persistence length of the complex decreases progressively during the complexation. Using astatistical model we describe the evolution of the flexibility of DNA complexed with NCp7. Our main result is that the fraction phi of base pairs that have reacted is not a linear function of time at low phi.We interpret our res
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32

Hopkinson, Devan. "Bioresponsive liposomes to target drug release in alveolar macrophages." Thesis, University of Manchester, 2017. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.713597.

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Tuberculosis is one of the most prevalent infectious diseases globally due to the successful survival mechanisms displayed by Mycobacterium tuberculosis (Mtb). Mtb primarily infects alveolar macrophages (AMs) and is able to live intracellularly for extended periods of time due to a number of virulence factors which inhibit the antibacterial mechanisms of the AMs. This aspect of the Mtb life cycle means TB treatments suffer from poor bioavailability and efficacy. Additionally, the rise in resistant strains of Mtb means the use of higher doses and the use of alternative second and third line dru
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33

Iordanov, Iordan. "Structure and dynamics of the outer membrane protein A from Klebsiella pneumoniae : a joint NMR–SMFS–proteolysis and MS approach." Toulouse 3, 2012. http://thesesups.ups-tlse.fr/1602/.

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"KpOmpA est une protéine de la membrane externe de K. Pneumoniae. Elle fait partie de la famille des "outer membrane protein A", OmpA. KpOmpA est une protéine constituée de deux domaines: un domaine transmembranaire structuré en tonneau ß et une partie soluble, périplasmique. Le domaine transmembranaire de KpOmpA présente une homologie importante avec celle d'OmpA d'E. Coli dont la structure a été déterminée par cristallographie aux rayons X et spectroscopie RMN. OmpA d'E. Coli est responsable lors de la formation de biofilm. Elle a un rôle d'adhésine et d'invasine. Elle est la cible préférent
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34

Roussel, Céline. "Etude du rôle des chélateurs calciques sur les oscillations du potentiel membranaire neuronal: approche expérimentale et théorique." Doctoral thesis, Universite Libre de Bruxelles, 2006. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210854.

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Les neurones sont des cellules excitables capables de coder et transmettre l’information sous forme d’oscillations du potentiel membranaire. Cette activité électrique est produite par une modification des flux ioniques transmembranaires. Les neurones constituent un exemple d’oscillateur cellulaire dont la dynamique non linéaire permet l’apparition d’une activité électrique complexe. Dans ce système, les ions calciques sont des messagers intracellulaires importants. Ils servent de médiateur entre un signal électrique et un signal chimique, par une modulation de l’activité enzymatique de certain
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35

Schwarzfischer, Michael [Verfasser], Fabian J. [Akademischer Betreuer] Theis, and Hans-Werner [Akademischer Betreuer] Mewes. "Quantification and analysis of single-cell protein dynamics in stem cells using time-lapse microscopy / Michael Schwarzfischer. Gutachter: Fabian J. Theis ; Hans-Werner Mewes. Betreuer: Fabian J. Theis." München : Universitätsbibliothek der TU München, 2014. http://d-nb.info/1060482452/34.

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36

Pasqualin, Côme. "Dynamique calcique dans les cardiomyocytes de veines pulmonaires de rat : une hétérogénéité source d'arythmie ?" Thesis, Tours, 2016. http://www.theses.fr/2016TOUR3808/document.

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Les activités électriques ectopiques à l’origine des épisodes de fibrillation atriale pourraient être dues à des échanges calciques anormaux dans les cardiomyocytes (CM) de veine pulmonaire (VP). Le cycle du calcium des CM de VP a donc été caractérisé et comparé à celui des CM d’oreillette gauche (OG) et de ventricule gauche (VG). Des outils ont été développés pour mesurer la régularité d’organisation des réseaux de tubules transverses et la contractilité des CM de VP. Contrairement aux CM d’OG et de VG, l’organisation hétérogène du réseau de tubules dans la population des CM de VP conduit à u
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37

Chen, X. "TAGGING BIOCONTROL STREPTOMYCES TO STUDY LETTUCE COLONIZATION." Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/345187.

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The ability of the biological control agents (BCAs) to colonize plant tissues is an important feature involved in microbe-assisted plant protection. Plant-microbe interaction research increased especially in the last decade thanks to technological revolution. Molecular methods and the development of advanced microscopic techniques allow researchers to explore gene expression and localization of beneficial microorganisms within plants. The green fluorescent protein (GFP) and its modified version, enhanced GFP (EGFP), more adapt for expression in mammalian cells and GC-rich actinomycetes like St
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38

D'Augustin, de Bourguisson Ostiane. "Caractérisation de la dynamique de l'ADN-glycosylase OGG1 et de résidus responsables de son interaction avec l'ADN en cellules vivantes." Electronic Thesis or Diss., Rennes 1, 2022. http://www.theses.fr/2022REN1B060.

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L’ADN est constamment soumis à de nombreux stress, menaçant son intégrité et, par conséquent, le bon fonctionnement cellulaire. Pour y faire face, la cellule dispose de tout un arsenal de voies de réparation. L’une des altération du génome les plus fréquentes est l’oxydation de la guanine en 8-oxoguanine (8-oxoG). La 8-oxoG possède un fort potentiel mutagène du fait de son appariement préférentiel avec l’adénine au lieu de la cytosine lors de la réplication. Ainsi, elle doit être détectée et réparée à temps pour éviter la fixation dans le génome de mutation par transversion G:C vers T:A. Cette
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39

Balakrishnan, Nair Gireeshkumar. "Particle diffusion in protein gels and at interfaces." Thesis, Le Mans, 2012. http://www.theses.fr/2012LEMA1002/document.

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L'objectif de la thèse était d'étudier la mobilité de traceurs particulaires dans des milieuxcomplexes par microscopie confocale à balayage laser (CLSM) combinée avec le suivi demultiple particules (MPT) et le recouvrement de fluorescence après photoblanchiment (FRAP).Tout d'abord, nous avons étudié la diffusion de particules dans les gels formés par des protéinesglobulaires. Dans ce but, des gels avec structures variés ont été préparés en faisant varier lesconcentrations en protéine et en sel. La structure a été caractérisée par l'analyse des imagesobtenues par CLSM en termes de fonction de c
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40

Teeling-Smith, Richelle Marie. "Single Molecule Electron Paramagnetic Resonance and Other Sensing and Imaging Applications with Nitrogen-Vacancy Nanodiamond." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1424779811.

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41

Collins, Patrick. "The Characterisation of Putative Nuclear Pore-Anchoring Proteins in Arabidopsis thaliana." Thesis, University of Canterbury. Biological Sciences, 2013. http://hdl.handle.net/10092/8885.

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The nuclear pore complex (NPC) is perhaps the largest protein complex in the eukaryotic cell, and controls the movement of molecules across the nuclear envelope. The NPC is composed of up to 30 proteins termed nucleoporins (Nups), each grouped in different sub-complexes. The transmembrane ring sub-complex is composed of Nups responsible for anchoring the NPC to the nuclear envelope. Bioinformatic analysis has traced all major sub-complexes of the NPC back to the last eukaryotic common ancestor, meaning that the nuclear pore structure and function is conserved amongst all eukaryotes. In this st
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42

Jouanny-Bouyer, Eléonore. "Stabilisation d’émulsions d’intérêt pharmaceutique par des protéines et des polysaccharides : exemples de la β-lactoglobuline, de la gomme arabique et de la gomme xanthane". Thesis, Paris 11, 2011. http://www.theses.fr/2011PA114802/document.

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L’objectif de cette étude a été de formuler et caractériser des émulsions simples huile/eau d’intérêt pharmaceutique stabilisées par de la β-lactoglobuline (β-lg), de la gomme arabique (GA), de la gomme xanthane (GX) et des mélanges β-lg:GA et β-lg:GX. Les concentrations massiques totales des dispersions de biopolymères étaient de 1 % et ont été augmentées à 2,5 % si les émulsions formulées n’étaient pas stables. Le mélange β-lg:GA a été réalisé à pH 4,2 afin de permettre la formation de complexes par interactions électrostatiques attractives entre la β-lg et la GA. Deux ratios β-lg:GA ont été
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43

Schwenen, Lando Lantbert Gregor. "Untersuchung einzelner SNARE-vermittelter Membranfusionsereignisse auf planaren porenüberspannenden Membranen." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0023-9650-F.

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44

Rino, José. "Dynamics and interactions of nuclear proteins revealed by quantitative photobleaching microscopy." Doctoral thesis, 2007. http://hdl.handle.net/10451/3329.

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Tese de doutoramento em Biofísica (Biofísica), apresentada à Universidade de Lisboa através da Faculdade de Ciências, 2007<br>The nucleus is a complex cellular organelle, exhibiting a high degree of organization and also a highly dynamic nature. Live cell imaging using fluorescent proteins (FPs) as molecular tags and photobleaching techniques have been essential in revealing the dynamic nature of the cell nucleus. In this thesis, these tools were used to study molecular dynamics and interactions inside this cellular compartment. Fluorescence Recovery After Photobleaching (FRAP) and Fluorescen
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45

Ge, Yifan. "Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques." Thesis, 2016. https://doi.org/10.7912/C2765K.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Plasma membranes are complex supramolecular assemblies comprised of lipids and membrane proteins. Both types of membrane constituents are organized in highly dynamic patches with profound impact on membrane functionality, illustrating the functional importance of plasma membrane fluidity. Exemplary, dynamic processes of membrane protein oligomerization and distribution are of physiological and pathological importance. However, due to the complexity of the plasma membrane, the underlying regulatory mechanisms of membrane protein organi
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46

Kuo, Kai-Wen, and 郭開文. "Application of Using Differential Confocal Microscopy with Optical Tweezers in Protein Elasticity Research." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/84727123743036163138.

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碩士<br>國立陽明大學<br>生醫光電工程研究所<br>93<br>Since the first invention in 1970 by Ashkin, optical tweezers have become a versatile non-invasive tool extensively used in controlling tiny biological samples, especially cytoskeleton related proteins. In optical tweezers, the precise measurement of axial motion of a trapped object in optical tweezers is not as well developed as that of the lateral motion. In this thesis we have introduced differential confocal microscopy (DCM), developed by Lee in 1997, into optical tweezers for the axial detection. DCM has advantageous features such as high depth resolutio
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47

Edwards, Amanda Nicole. "Microscopy Techniques for Investigating Interactions in Microbial Systems." 2011. http://trace.tennessee.edu/utk_graddiss/964.

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Biological interactions occur on multiple length scales, ranging from molecular to population wide interactions. This work describes the study of two specific areas of biological interactions in microbial systems: intracellular protein-protein interactions and cell-to-cell interactions. The implementation of optical and atomic force microscopy and the methodologies developed during this study proved to be invaluable tools for investigating these systems. Identifying and characterizing protein interactions are fundamental steps toward understanding complex cellular networks. We have developed
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Khmelinskaia, Alena. "Protein-monolayer interactions investigated by fluorescence microscopy and correlation spectroscopy." Master's thesis, 2013. http://hdl.handle.net/10451/9654.

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Tese de mestrado em Bioquímica, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2013<br>A membrana celular é composta por uma ampla variedade de lípidos e proteínas. Através do estabelecimento de interacções entre lípidos e proteínas, quer membranares quer solúveis, podem ser originadas heterogeneidades espaciais, levando à formação de ‘jangadas lipídicas’. Estes domínios transitórios desempenham um papel fundamental em diferentes cadeias de sinalização celular. Apesar da investigação desenvolvida na área, a membrana celular é ainda hoje um dos componentes celulares men
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Haselbach, David. "Conformational Dynamics of large protein Complexes." Doctoral thesis, 2014. http://hdl.handle.net/11858/00-1735-0000-0022-5DC2-1.

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Luensmann, Doerte. "Protein sorption to contact lenses and intraocular lenses." Thesis, 2009. http://hdl.handle.net/10012/4879.

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Purpose: To locate protein sorption on the surface and inside the matrix of soft contact lens materials and intraocular lenses (IOL). Methods: The proteins albumin and lysozyme were investigated as they are highly abundant in blood serum and tears, respectively. Proteins were conjugated with organic fluorescent probes and using confocal laser scanning microscopy (CLSM) the sorption profile to contact lenses and IOL could be determined. Radiolabeled protein was used for quantification purposes. • Albumin sorption to etafilcon A and lotrafilcon B was determined (Chapter 3) • Different fluoresce
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