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Journal articles on the topic 'Protein expression; Fusobacterium nucleatum; biofilm'

Author: Grafiati

Published: 10 December 2022

Last updated: 31 July 2025

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1

Schnurr, Etyene, Pune N. Paqué, Thomas Attin, et al. "Staphylococcus aureus Interferes with Streptococci Spatial Distribution and with Protein Expression of Species within a Polymicrobial Oral Biofilm." Antibiotics 10, no. 2 (2021): 116. http://dx.doi.org/10.3390/antibiotics10020116.

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We asked whether transient Staphylococcus aureus in the oral environment synergistically interacts with orally associated bacterial species such as Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus mutans, and Veillonella dispar (six-species control biofilm 6S). For this purpose, four modified biofilms with seven species that contain either the wild type strain of the S. aureus genotype (USA300-MRSA WT), its isogenic mutant with MSCRAMM deficiency (USA300-MRSA ΔMSCRAMM), a methicillin-sensitive S. aureus (ST72-MSSA-) or a methicillin-resistant S.

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Sasaki-Imamura, Takako, Akira Yano, and Yasuo Yoshida. "Production of Indole from l-Tryptophan and Effects of These Compounds on Biofilm Formation by Fusobacterium nucleatum ATCC 25586." Applied and Environmental Microbiology 76, no. 13 (2010): 4260–68. http://dx.doi.org/10.1128/aem.00166-10.

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ABSTRACT The l-tryptophan degradation product indole is a purported extracellular signaling molecule that influences biofilm formation in various bacteria. Here we analyzed the mechanisms of indole production in Fusobacterium nucleatum and the effects of tryptophan and indole on F. nucleatum planktonic and biofilm cells. The amino acid sequence deduced from the fn1943 gene in F. nucleatum ATCC 25586 was 28% identical to that deduced from tnaA in Escherichia coli, which encodes tryptophanase catalyzing the β-elimination of l-tryptophan to produce indole. The fn1943 gene was cotranscribed with t

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Honma, Kiyonobu, Elina Mishima, Satoru Inagaki, and Ashu Sharma. "The OxyR homologue in Tannerella forsythia regulates expression of oxidative stress responses and biofilm formation." Microbiology 155, no. 6 (2009): 1912–22. http://dx.doi.org/10.1099/mic.0.027920-0.

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Tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. In this study, we sought to characterize a T. forsythia ORF with close similarity to bacterial oxidative stress response sensor protein OxyR. To analyse the role of this OxyR homologue, a gene deletion mutant was constructed and characterized. Aerotolerance, survival after hydrogen peroxide challenge and transcription levels of known bacterial antioxidant g

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Fong, Karen P., Whasun O. Chung, Richard J. Lamont, and Donald R. Demuth. "Intra- and Interspecies Regulation of Gene Expression by Actinobacillus actinomycetemcomitansLuxS." Infection and Immunity 69, no. 12 (2001): 7625–34. http://dx.doi.org/10.1128/iai.69.12.7625-7634.2001.

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ABSTRACT The cell density-dependent control of gene expression is employed by many bacteria for regulating a variety of physiological functions, including the generation of bioluminescence, sporulation, formation of biofilms, and the expression of virulence factors. Although periodontal organisms do not appear to secrete acyl-homoserine lactone signals, several species, e.g., Porphyromonas gingivalis,Prevotella intermedia, and Fusobacterium nucleatum, have recently been shown to secrete a signal related to the autoinducer II (AI-2) of the signal system 2 pathway inVibrio harveyi. Here, we repo

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Bachtiar, Endang W., Boy M. Bachtiar, Ardiana Kusumaningrum, et al. "ACE2 expression in saliva of patients with COVID-19 and its association with Candida albicans and Aggregatibacter actinomycetemcomitans." F1000Research 11 (May 23, 2022): 557. http://dx.doi.org/10.12688/f1000research.111965.1.

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Background: A relationship between oral microbiota and susceptibility to SARS-CoV-2 infection has been extensively studied. However, the relationship between oral commensal flora and expression of angiotensin-converting enzyme 2 (ACE2) remains to be established. In this observational study, we collected saliva from patients with COVID-19 and evaluated the relationship between ACE2 expression and Candida albicans as well as with selected gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Veillonella parvula). We investigated how this may be directly or i

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Bachtiar, Endang W., Boy M. Bachtiar, Ardiana Kusumaningrum, et al. "ACE2 expression in saliva of patients with COVID-19 and its association with Candida albicans and Aggregatibacter actinomycetemcomitans." F1000Research 11 (September 12, 2022): 557. http://dx.doi.org/10.12688/f1000research.111965.2.

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Background: A relationship between oral microbiota and susceptibility to SARS-CoV-2 infection has been extensively studied. However, the relationship between oral commensal flora and expression of angiotensin-converting enzyme 2 (ACE2) remains to be established. In this observational study, we collected saliva from patients with COVID-19 and evaluated the relationship between ACE2 expression and Candida albicans as well as with selected gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Veillonella parvula). We investigated how this may be directly or i

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Kaplan, C. W., R. Lux, T. Huynh, A. Jewett, W. Shi, and S. Kinder Haake. "Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein." Journal of Dental Research 84, no. 8 (2005): 700–704. http://dx.doi.org/10.1177/154405910508400803.

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The periodontal pathogen Fusobacterium nucleatum induces apoptosis in lymphocytes. We previously identified the autotransporter protein Fap2 in F. nucleatum strain PK1594 that induced apoptosis in lymphocytes when expressed in Escherichia coli. In this study, we identified protein homologs of Fap2 in the transformable F. nucleatum strain ATCC 23726, to determine their role in the induction of apoptosis in lymphocytes. We used a new gene-inactivation vector conferring thiamphenicol resistance (pHS31) to construct a mutant deficient in one of the homologs, aim1. Transcriptional analyses demonstr

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Mendes, Reila Tainá, Daniel Nguyen, Danielle Stephens, et al. "Endothelial Cell Response to Fusobacterium nucleatum." Infection and Immunity 84, no. 7 (2016): 2141–48. http://dx.doi.org/10.1128/iai.01305-15.

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Vascular response is an essential aspect of an effective immune response to periodontal disease pathogens, as new blood vessel formation contributes to wound healing and inflammation. Gaining a greater understanding of the factors that affect vascular response may then contribute to future breakthroughs in dental medicine. In this study, we have characterized the endothelial cell response to the common bacteriumFusobacterium nucleatum, an important bridging species that facilitates the activity of late colonizers of the dental biofilm. Endothelial cells were infected withFusobacterium nucleatu

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Uitto, Veli-Jukka, Daniel Baillie, Qiang Wu, et al. "Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells." Infection and Immunity 73, no. 2 (2005): 1171–79. http://dx.doi.org/10.1128/iai.73.2.1171-1179.2005.

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ABSTRACT Fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. Initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. Production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. We studied binding of F. nucleatum to human epithelial cells (HaCaT keratinocyte line) and subsequent cell signaling related to collagenase

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Bachrach, Gilad, Susan Kinder Haake, Alon Glick, et al. "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System." Applied and Environmental Microbiology 70, no. 12 (2004): 6957–62. http://dx.doi.org/10.1128/aem.70.12.6957-6962.2004.

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ABSTRACT Fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. Studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. Plasmids were isolated from eight strains of F. nucleatum. The smallest plasmid, pKH9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. DNA sequence analysis of pKH9 revealed an open reading frame (ORF) encoding a putative autonomous rolling circle replication protein

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Cores Ziskoven, Pablo, Andressa V. B. Nogueira, Lorena S. Gutierrez, et al. "Apelin Enhances the Effects of Fusobacterium nucleatum on Periodontal Ligament Cells In Vitro." International Journal of Molecular Sciences 24, no. 5 (2023): 4733. http://dx.doi.org/10.3390/ijms24054733.

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This study aimed to explore effects of Fusobacterium nucleatum with or without apelin on periodontal ligament (PDL) cells to better understand pathomechanistic links between periodontitis and obesity. First, the actions of F. nucleatum on COX2, CCL2, and MMP1 expressions were assessed. Subsequently, PDL cells were incubated with F. nucleatum in the presence and absence of apelin to study the modulatory effects of this adipokine on molecules related to inflammation and hard and soft tissue turnover. Regulation of apelin and its receptor (APJ) by F. nucleatum was also studied. F. nucleatum resul

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Lee, Seok-Joo, та Bong-Kyu Choi. "Involvement of NLRP10 in IL-1α induction of oral epithelial cells by periodontal pathogens". Innate Immunity 23, № 7 (2017): 569–77. http://dx.doi.org/10.1177/1753425917722610.

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This study investigated the pathogenesis of periodontitis and the role of nucleotide-binding oligomerization domain-like receptor protein 10 (NLRP10). The human oral epithelial cell line HOK-16B was infected with two periodontal pathogens, Tannerella forsythia and Fusobacterium nucleatum, at various MOIs. RT-PCR and immunoblotting demonstrated that infection increased mRNA and protein expression of NLRP10, respectively. The siRNA-mediated NLRP10 knockdown significantly reduced IL-1α expression and secretion. Both bacteria induced phosphorylation of ERK, JNK and p38 MAP kinases in HOK-16B cells

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Zhou, Ting, Xianhong Meng, Daxiu Wang, Weiran Fu, and Xinrui Li. "Neutrophil Transcriptional Deregulation by the Periodontal Pathogen Fusobacterium nucleatum in Gastric Cancer: A Bioinformatic Study." Disease Markers 2022 (August 18, 2022): 1–10. http://dx.doi.org/10.1155/2022/9584507.

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Background. Infection with the periodontal pathogen Fusobacterium nucleatum (F. nucleatum) has been associated with gastric cancer. The present study is aimed at uncovering the putative biological mechanisms underlying effects of F. nucleatum–mediated neutrophil transcriptional deregulation in gastric cancer. Materials and Methods. A gene expression dataset pertaining to F. nucleatum-infected human neutrophils was utilized to identify differentially expressed genes (DEGs) using the GEO2R tool. Candidate genes associated with gastric cancer were sourced from the “Candidate Cancer Gene Database”

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Ji, Suk, Ji Eun Shin, Young Sook Kim, Ju-Eun Oh, Byung-Moo Min, and Youngnim Choi. "Toll-Like Receptor 2 and NALP2 Mediate Induction of Human Beta-Defensins by Fusobacterium nucleatum in Gingival Epithelial Cells." Infection and Immunity 77, no. 3 (2008): 1044–52. http://dx.doi.org/10.1128/iai.00449-08.

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ABSTRACT We previously reported that infection by Fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (HBD-2) and HBD-3 by gingival epithelial cells. The aim of this study was to characterize the pattern recognition receptors (PRRs) and regulatory mechanisms involved in the induction of HBD-2 and -3 expression by F. nucleatum in gingival epithelial cells. Immortalized human gingival epithelial HOK-16B cells were infected with live or heat-killed F. nucleatum, and the expression of HBDs and interleukin-8 (IL-8) was examined by real-time reverse transcription-PC

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Nogueira, Andressa V. B., Marjan Nokhbehsaim, Anna Damanaki, et al. "Effect of Bacterial Infection on Ghrelin Receptor Regulation in Periodontal Cells and Tissues." International Journal of Molecular Sciences 23, no. 6 (2022): 3039. http://dx.doi.org/10.3390/ijms23063039.

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The effect of bacterial infection on the expression of growth hormone secretagogue receptor (GHS-R) was investigated in periodontal cells and tissues, and the actions of ghrelin were evaluated. GHS-R was assessed in periodontal tissues of rats with and without periodontitis. Human gingival fibroblasts (HGFs) were exposed to Fusobacterium nucleatum in the presence and absence of ghrelin. GHS-R expression was determined by real-time PCR and immunocytochemistry. Furthermore, wound healing, cell viability, proliferation, and migration were evaluated. GHS-R expression was significantly higher at pe

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Hung, Shu-Chen, Pei-Rong Huang, Fiona Q. Bui, and David Ojcius. "NLRX1 modulates the balance between NLRP3 inflammasome activation and NF-kB signaling during Fusobacterium nucleatum infection." Journal of Immunology 196, no. 1_Supplement (2016): 62.1. http://dx.doi.org/10.4049/jimmunol.196.supp.62.1.

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Abstract NOD-like receptors (NLRs) play an important role in regulation of host innate immunity, yet their role in periodontitis remains to be defined. NLRX1, a member of the NLR family that localizes to mitochondria, modulates mitochondrial ROS (mROS) generation. mROS has been shown to activate the NLRP3 inflammasome, yet the role of NLRX1 in NLRP3 inflammasome activation has not been examined. In this study, we revealed the mechanism by which NLRX1 positively regulates ATP-elicited NLRP3 inflammasome activation through mROS in gingival epithelial cells (GECs). Fluorescence microscopy showed

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Asai, Y., Y. Ohyama, Y. Taiji, et al. "Treponema medium Glycoconjugate Inhibits Activation of Human Gingival Fibroblasts Stimulated with Phenol-Water Extracts of Periodontopathic Bacteria." Journal of Dental Research 84, no. 5 (2005): 456–61. http://dx.doi.org/10.1177/154405910508400511.

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Oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. Here, we examined the effects of Treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum subsp. nucleatum, and Actinobacillus actinomycetemcomitans. The phenol-water extracts activated human gingival fibroblasts to mediate IL-8 production, as well as IL-8 mRNA expression, phosphorylation of p38 mitogen-activated protein kinase, and expressi

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Zhao, Tian, Jiaqi Chen, Shuai Liu, et al. "Transcriptome analysis of Fusobacterium nucleatum reveals differential gene expression patterns in the biofilm versus planktonic cells." Biochemical and Biophysical Research Communications 593 (February 2022): 151–57. http://dx.doi.org/10.1016/j.bbrc.2021.11.075.

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Kim, D. J., J. H. Rho, B. H. Woo, et al. "Periodontal Pathogens Modulate Lipid Flux via Fatty Acid Binding Protein 4." Journal of Dental Research 98, no. 13 (2019): 1511–20. http://dx.doi.org/10.1177/0022034519880824.

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A strong correlation between chronic periodontitis and systemic diseases (e.g., cardiovascular disease, metabolic disorders) has been suggested for several decades. However, the evidence supporting this correlation is restricted primarily to epidemiologic studies, with only a few experimental outcomes confirming such a correlation and providing information about the underlying molecular mechanisms. To reveal a correlation between periodontitis and systemic diseases as well as a relevant molecular pathway, we investigated the effects of Porphyromonas gingivalis and Fusobacterium nucleatum, whic

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Scano, Alessandra, Sara Fais, Giuliana Ciappina, et al. "Oxidative Stress by H2O2 as a Potential Inductor in the Switch from Commensal to Pathogen in Oncogenic Bacterium Fusobacterium nucleatum." Antioxidants 14, no. 3 (2025): 323. https://doi.org/10.3390/antiox14030323.

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Background: Fusobacterium nucleatum is a pathobiont that plays a dual role as both a commensal and a pathogen. The oral cavity typically harbors this anaerobic, Gram-negative bacterium. At the same time, it is closely linked to colorectal cancer due to its potential involvement in tumor progression and resistance to chemotherapy. The mechanism by which it transforms from a commensal to a pathogen remains unknown. For this reason, we investigated the role of oxidative status as an initiatory factor in changing the bacterium’s pathogenicity profile. Methods: A clinical strain of F. nucleatum sub

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Padma, Somrita, Ritwik Patra, Parth Sarthi Sen Gupta, Saroj Kumar Panda, Malay Kumar Rana, and Suprabhat Mukherjee. "Cell Surface Fibroblast Activation Protein-2 (Fap2) of Fusobacterium nucleatum as a Vaccine Candidate for Therapeutic Intervention of Human Colorectal Cancer: An Immunoinformatics Approach." Vaccines 11, no. 3 (2023): 525. http://dx.doi.org/10.3390/vaccines11030525.

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Colorectal cancer (CRC) is one of the most common cancers and is the second-highest in cancer-related deaths worldwide. The changes in gut homeostasis and microbial dysbiosis lead to the initiation of the tumorigenesis process. Several pathogenic gram-negative bacteria including Fusobacterium nucleatum are the principal contributors to the induction and pathogenesis of CRC. Thus, inhibiting the growth and survival of these pathogens can be a useful intervention strategy. Fibroblast activation protein-2 (Fap2) is an essential membrane protein of F. nucleatum that promotes the adherence of the b

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Al-Ahmad, Ali, Kira Wollensak, Sibylle Rau, et al. "How Do Polymer Coatings Affect the Growth and Bacterial Population of a Biofilm Formed by Total Human Salivary Bacteria?—A Study by 16S-RNA Sequencing." Microorganisms 9, no. 7 (2021): 1427. http://dx.doi.org/10.3390/microorganisms9071427.

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Antimicrobial surface modifications are required to prevent biomaterial-associated biofilm infections, which are also a major concern for oral implants. The aim of this study was to evaluate the influence of three different coatings on the biofilm formed by human saliva. Biofilms grown from human saliva on three different bioactive poly(oxanorbornene)-based polymer coatings (the protein-repellent PSB: poly(oxanorbornene)-based poly(sulfobetaine), the protein-repellent and antimicrobial PZI: poly(carboxyzwitterion), and the mildly antimicrobial and protein-adhesive SMAMP: synthetic mimics of an

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Karacic, Jelena, Moritz Ruf, Johannes Herzog, Monika Astasov-Frauenhoffer, and Philipp Sahrmann. "Effect of Dentifrice Ingredients on Volume and Vitality of a Simulated Periodontal Multispecies Biofilm." Dentistry Journal 12, no. 5 (2024): 141. http://dx.doi.org/10.3390/dj12050141.

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The aim of this in vitro study was to investigate the effect of different toothpaste ingredients on biofilm volume and vitality in an established non-contact biofilm removal model. A multi-species biofilm comprising Porphyromonas gingivalis, Streptococcus sanguinis, and Fusobacterium nucleatum was grown on protein-coated titanium disks. Six disks per group were exposed to 4 seconds non-contact brushing using a sonic toothbrush. Four groups assessed slurries containing different ingredients, i.e., dexpanthenol (DP), peppermint oil (PO), cocamidopropyl betaine (CB), and sodium hydroxide (NaOH),

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Hasegawa, Yoshiaki, Jeffrey J. Mans, Song Mao, et al. "Gingival Epithelial Cell Transcriptional Responses to Commensal and Opportunistic Oral Microbial Species." Infection and Immunity 75, no. 5 (2007): 2540–47. http://dx.doi.org/10.1128/iai.01957-06.

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ABSTRACT Transcriptional profiling and ontology tools were utilized to define the biological pathways of gingival epithelial cells modulated by coculture with the oral commensal Streptococcus gordonii and the opportunistic commensal Fusobacterium nucleatum. Overall, F. nucleatum and S. gordonii perturbed the gingival epithelial cell transcriptome much less significantly than the oral pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans perturbed the transcriptome, indicating that there was a greater degree of host adaptation by the commensal species (M. Handfield, J. J.

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ZHONG, FANGCHAO, XIAOXIA WEI, MAOSEN HUANG, et al. "Significance of Fusobacterium nucleatum Combined with SFRP2 and SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer." Polish Journal of Microbiology 74, no. 2 (2025): 218–31. https://doi.org/10.33073/pjm-2025-018.

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Abstract This study aimed to explore the combined detection of secreted frizzled-related protein-2 (SFRP2), Syndecan-2 (SDC2), and Fusobacterium nucleatum (Fn) in fecal samples for early colorectal cancer (CRC) screening. Public datasets were analyzed to evaluate the expression of SFRP2, SDC2, and Fn. The study included 30 pairs of tissue and 196 fecal samples. Gene expression of SFRP2, SDC2, F. nucleatum antigen adhesinA (fadA), and N-utilization substance G (nusG) was measured by qPCR. Correlations with Ki67, P53 expression, and immune infiltration were examined. The diagnostic performance o

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Haake, Susan Kinder, and Xiurong Wang. "Cloning and expression of FomA, the major outer-membrane protein gene from fusobacterium nucleatum T18." Archives of Oral Biology 42, no. 1 (1997): 19–24. http://dx.doi.org/10.1016/s0003-9969(96)00105-7.

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Zhang, Qian, Shuipeng Yu, Meilin Hu, et al. "Antibacterial and Anti-Inflammatory Properties of Peptide KN-17." Microorganisms 10, no. 11 (2022): 2114. http://dx.doi.org/10.3390/microorganisms10112114.

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Peri-implantitis, an infectious disease originating from dental biofilm that forms around dental implants, which causes the loss of both osseointegration and bone tissue. KN-17, a truncated cecropin B peptide, demonstrated efficacy against certain bacterial strains associated with peri-implantitis. This study aimed to assess the antibacterial and anti-inflammatory properties and mechanisms of KN-17. The effects of KN-17 on oral pathogenic bacteria were assessed by measuring its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Moreover, the cytotoxicity and a

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Memmert, S., A. Damanaki, A. V. B. Nogueira, et al. "Role of Cathepsin S in Periodontal Inflammation and Infection." Mediators of Inflammation 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/4786170.

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Cathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and infectious conditions elicited by interleukin-1β and Fusobacterium nucleatum, respectively. An array-based approach was used to analyze differential expression of autophagy-associated genes. Cathepsin S was upregulated most strongly and thus further studied in vitro at gene and protein levels. In vivo,

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Yoshida, Yasuo, Shuntaro Ito, Masaharu Kamo, et al. "Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine in Fusobacterium nucleatum subsp. nucleatum ATCC 25586." Microbiology 156, no. 7 (2010): 2260–69. http://dx.doi.org/10.1099/mic.0.039180-0.

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Fusobacterium nucleatum produces a large amount of the toxic metabolite hydrogen sulfide in the oral cavity. Here, we report the molecular basis of F. nucleatum H2S production, which is associated with two different enzymes: the previously reported Cdl (Fn1220) and the newly identified Lcd (Fn0625). SDS-PAGE analysis with activity staining revealed that crude enzyme extracts from F. nucleatum ATCC 25586 contained three major H2S-producing proteins. Two of the proteins with low molecular masses migrated similarly to purified Fn0625 and Fn1220. Their kinetic values suggested that Fn0625 had a lo

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Kang, Mi-Sun, Geun-Yeong Park, and A.-Reum Lee. "In Vitro Preventive Effect and Mechanism of Action of Weissella cibaria CMU against Streptococcus mutans Biofilm Formation and Periodontal Pathogens." Microorganisms 11, no. 4 (2023): 962. http://dx.doi.org/10.3390/microorganisms11040962.

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In this study, we evaluated the in vitro anti-biofilm, antibacterial, and anti-inflammatory activity of Weissella cibaria CMU (CMU), an oral probiotic, against periodontopathogens. Compared to other oral probiotics, CMU showed a superior inhibitory effect on the biofilm formation and growth of Streptococcus mutans on orthodontic wires and artificial teeth (p < 0.05). CMU exerted potent antibacterial effects against S. mutans and Porphyromonas gingivalis according to a line test. In human gingival fibroblasts (HGFs) stimulated by P. gingivalis, Fusobacterium nucleatum, or Prevotella intermed

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Zilm, Peter S., Alex Mira, Christopher J. Bagley, and Anthony H. Rogers. "Effect of alkaline growth pH on the expression of cell envelope proteins in Fusobacterium nucleatum." Microbiology 156, no. 6 (2010): 1783–94. http://dx.doi.org/10.1099/mic.0.035881-0.

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Fusobacterium nucleatum is a Gram-negative anaerobic organism that plays a central role in the development of periodontal diseases. The progression of periodontitis is associated with a rise in pH of the gingival sulcus which promotes the growth and expression of virulence factors by periodontopathic bacteria. We have previously reported that the expression of specific cytoplasmic proteins is altered by a shift in growth pH. In the present study we have compared cell envelope protein expression of F. nucleatum during chemostat growth at pH 7.2 and 7.8. From a total of 176 proteins resolved fro

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Krisanaprakornkit, Suttichai, Janet R. Kimball, Aaron Weinberg, Richard P. Darveau, Brian W. Bainbridge та Beverly A. Dale. "Inducible Expression of Human β-Defensin 2 byFusobacterium nucleatum in Oral Epithelial Cells: Multiple Signaling Pathways and Role of Commensal Bacteria in Innate Immunity and the Epithelial Barrier". Infection and Immunity 68, № 5 (2000): 2907–15. http://dx.doi.org/10.1128/iai.68.5.2907-2915.2000.

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ABSTRACT Human gingival epithelial cells (HGE) express two antimicrobial peptides of the β-defensin family, human β-defensin 1 (hBD-1) and hBD-2, as well as cytokines and chemokines that contribute to innate immunity. In the present study, the expression and transcriptional regulation of hBD-2 was examined. HBD-2 mRNA was induced by cell wall extract of Fusobacterium nucleatum, an oral commensal microorganism, but not by that of Porphyromonas gingivalis, a periodontal pathogen. HBD-2 mRNA was also induced by the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) and phorbol myristate

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Rath-Deschner, Birgit, Svenja Memmert, Anna Damanaki, et al. "CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies." Clinical Oral Investigations 24, no. 10 (2020): 3661–70. http://dx.doi.org/10.1007/s00784-020-03244-1.

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Abstract Objectives This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. Materials and methods The chemokine expression and protein levels in gingival biopsies from patients with and without periodontitis were analyzed by RT-PCR and immunohistochemistry. The chemokines were also analyzed in gingival biopsies from rats subjected to experimental periodontitis and/or orthodontic tooth movement. Additionally, chemokine levels were de

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Park, Jeong-Yong, Ji Yeon Lee, YongGyeong Kim, Byoung-Kook Kim, and Soo-Im Choi. "The Effect of Limosilactobacillus fermentum MG4717 on Oral Health and Biosafety." Microorganisms 13, no. 7 (2025): 1600. https://doi.org/10.3390/microorganisms13071600.

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Oral diseases such as periodontitis and dental caries, as well as conditions related to oral health such as halitosis, are closely associated with dysbiosis of the oral microbiota and continue to pose significant public health challenges worldwide. With the increasing resistance to existing antibiotics and side effects of chemical disinfectants, probiotics have emerged as promising alternatives for oral healthcare. This study aimed to evaluate the oral health efficacy and probiotic properties of Limosilactobacillus fermentum (L. fermentum) MG4717 isolated from the human oral cavity. L. ferment

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Gursoy, Ulvi K., Marja Pöllänen, Eija Könönen, and Veli-Jukka Uitto. "A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression." Journal of Periodontology 83, no. 2 (2012): 242–47. http://dx.doi.org/10.1902/jop.2011.110177.

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Huang, George T. J., Daniel Kim, Jonathan K. H. Lee, Howard K. Kuramitsu, and Susan Kinder Haake. "Interleukin-8 and Intercellular Adhesion Molecule 1 Regulation in Oral Epithelial Cells by Selected Periodontal Bacteria: Multiple Effects of Porphyromonas gingivalis via Antagonistic Mechanisms." Infection and Immunity 69, no. 3 (2001): 1364–72. http://dx.doi.org/10.1128/iai.69.3.1364-1372.2001.

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ABSTRACT Interaction of bacteria with mucosal surfaces can modulate the production of proinflammatory cytokines and adhesion molecules produced by epithelial cells. Previously, we showed that expression of interleukin-8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) by gingival epithelial cells increases following interaction with several putative periodontal pathogens. In contrast, expression of IL-8 and ICAM-1 is reduced after Porphyromonas gingivalis ATCC 33277 challenge. In the present study, we investigated the mechanisms that govern the regulation of these two molecules in bacteri

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Wu, Yixian, Songhe Guo, Fangfang Chen, et al. "Fn-Dps, a novel virulence factor of Fusobacterium nucleatum, disrupts erythrocytes and promotes metastasis in colorectal cancer." PLOS Pathogens 19, no. 1 (2023): e1011096. http://dx.doi.org/10.1371/journal.ppat.1011096.

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Fusobacterium nucleatum (Fn) is a critical colorectal cancer (CRC)-associated bacterium. DNA hunger/stationary phase protective proteins (Dps) are bacterial ferritins that protect DNA from oxidative stress. However, little is known about the regulatory roles of Fn-Dps towards host cellular functions. Here, we identified Fn-Dps from the culture supernatant of Fn by mass spectrometry, and prepared the recombinant of Fn-Dps protein. We show a novel virulence protein of Fn, Fn-Dps, which lyses and disrupts erythrocytes by the competition for iron acquisition. Also, Fn-Dps facilitates intracellular

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Ali Mohammed, Marwan Mansoor, Veronika Kuchařová Pettersen, Audun H. Nerland, Harald G. Wiker, and Vidar Bakken. "Label-free quantitative proteomic analysis of the oral bacteria Fusobacterium nucleatum and Porphyromonas gingivalis to identify protein features relevant in biofilm formation." Anaerobe 72 (December 2021): 102449. http://dx.doi.org/10.1016/j.anaerobe.2021.102449.

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Huang, Angela, Anjana Saxena, and Mintoo Patel. "Abstract 2202: Role of FadA secreting Fusobacterium nucleatum in initiation and progression of colorectal cancer." Cancer Research 83, no. 7_Supplement (2023): 2202. http://dx.doi.org/10.1158/1538-7445.am2023-2202.

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Abstract Colorectal cancer (CRC) is a global disease with poor prognosis. There is an alarming increase in incidence of CRC in younger patients along with well recognized racial disparities. Recent data implicate a role of microbiome in initiation and progression of CRC. Fusobacterium nucleatum (Fn), an oral opportunistic pathogen, has emerged as one of the common pathogens associated with CRC. Fn transformation from commensal to pathogen requires expression of amyloid-like FadA adhesin that plays a key role in its attachment to the colonic mucosal cells resulting in inflammatory, oncogenic, a

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Feng, Yu-yang, Dong-zhu Zeng, Ya-nan Tong, et al. "Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum." PLOS ONE 14, no. 4 (2019): e0215088. http://dx.doi.org/10.1371/journal.pone.0215088.

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Karaca, Basar, Ozan Haliscelik, Mervi Gursoy, et al. "Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics." Microorganisms 10, no. 11 (2022): 2200. http://dx.doi.org/10.3390/microorganisms10112200.

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Previous studies have indicated that the exopolysaccharides of lactic acid bacteria exhibit antibiofilm activity against non-oral bacteria by preventing their initial adhesion to surfaces and by downregulating the expression of genes responsible for their biofilm formation. The aims of this study were to (1) characterize the exopolysaccharides (EPSs) of Lactobacillus plantarum EIR/IF-1 postbiotics, (2) test their antibiofilm effect on dual biofilms, and (3) evaluate their bacterial auto-aggregation, co-aggregation, and hydrocarbon-binding inhibitory activity. The EPSs were characterized by FTI

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Bhattacharyya, Sanghamitra, Santosh K. Ghosh, Bhumika Shokeen, et al. "FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6." Infection and Immunity 84, no. 5 (2016): 1446–56. http://dx.doi.org/10.1128/iai.01311-15.

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We previously identified a cell wall-associated protein fromFusobacterium nucleatum, a Gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hBD-2) in primary human oral epithelial cells (HOECs) and designated it FAD-I (Fusobacterium-associateddefensininducer). Here, we report differential induction of hBD-2 by different strains ofF. nucleatum; ATCC 25586 and ATCC 23726 induce significantly more hBD-2 mRNA than ATCC 10953. Heterologous expression of plasmid-bornefadIfrom the highly hBD-2-inducing strains in a ΔfadImutant of ATCC 10953 resulted in hBD-2 induction to le

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Karaca, Fadime, Susanne Bloch, Fabian L. Kendlbacher, Christian Behm, Christina Schäffer, and Oleh Andrukhov. "Vitamin D3 Modulates Inflammatory and Antimicrobial Responses in Oral Epithelial Cells Exposed to Periodontitis-Associated Bacteria." International Journal of Molecular Sciences 26, no. 14 (2025): 7001. https://doi.org/10.3390/ijms26147001.

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The oral epithelium is essential for maintaining oral health and plays a key role in the onset and progression of periodontitis. It serves as both a mechanical and immunological barrier and possesses antimicrobial activity. Vitamin D3, a hormone with known immunomodulatory functions, may influence oral epithelial responses. This study investigated the effects of two vitamin D3 metabolites on key immunological and antimicrobial functions of oral epithelial cells, both under basal conditions and during bacterial challenge. Ca9-22 oral epithelial cells were treated with 1,25(OH)2D3 or 25(OH)D3 in

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Baqui, A. A. M. A., Timothy F. Meiller, Jennifer J. Chon, Been-Foo Turng та William A. Falkler. "Granulocyte-Macrophage Colony-Stimulating Factor Amplification of Interleukin-1β and Tumor Necrosis Factor Alpha Production in THP-1 Human Monocytic Cells Stimulated with Lipopolysaccharide of Oral Microorganisms". Clinical Diagnostic Laboratory Immunology 5, № 3 (1998): 341–47. http://dx.doi.org/10.1128/cdli.5.3.341-347.1998.

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ABSTRACT Cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF), are used to assist in bone marrow recovery during cancer chemotherapy. Interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) play important roles in inflammatory processes, including exacerbation of periodontal diseases, one of the most common complications in patients who undergo this therapy. A human monocyte cell line (THP-1) was utilized to investigate IL-1β and TNF-α production following GM-CSF supplementation with lipopolysaccharide (LPS) from two oral microorganisms, Porphyromonas gingivali

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Thumbigere Math, V., P. Rebouças, P. A. Giovani, et al. "Periodontitis in Chédiak-Higashi Syndrome: An Altered Immunoinflammatory Response." JDR Clinical & Translational Research 3, no. 1 (2017): 35–46. http://dx.doi.org/10.1177/2380084417724117.

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Chédiak-Higashi syndrome (CHS), a rare autosomal recessive disorder caused by mutations in the lysosomal trafficking regulator gene (LYST), is associated with aggressive periodontitis. It is suggested that LYST mutations affect the toll-like receptor (TLR)–mediated immunoinflammatory response, leading to frequent infections. This study sought to determine the periodontal status of patients with classic (severe) and atypical (milder) forms of CHS and the immunoregulatory functions of gingival fibroblasts in CHS patients. In contrast to aged-matched healthy controls, atypical (n = 4) and classic

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Yun, In-Gyeong, Sun-Hee Ahn, Weon-Jong Yoon та ін. "Litsea japonica Leaf Extract Suppresses Proinflammatory Cytokine Production in Periodontal Ligament Fibroblasts Stimulated with Oral Pathogenic Bacteria or Interleukin-1β". International Journal of Molecular Sciences 19, № 9 (2018): 2494. http://dx.doi.org/10.3390/ijms19092494.

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Periodontal disease, a chronic disease caused by bacterial infection, eventually progresses to severe inflammation and bone loss. Regulating excessive inflammation of inflamed periodontal tissues is critical in treating periodontal diseases. The periodontal ligament (PDL) is primarily a connective tissue attachment between the root and alveolar bone. PDL fibroblasts (PDLFs) produce pro-inflammatory cytokines in response to bacterial infection, which could further adversely affect the tissue and cause bone loss. In this study, we determined the ability of Litsea japonica leaf extract (LJLE) to

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Loeurng, Vandara, Sao Puth, Seol Hee Hong, et al. "A Flagellin-Adjuvanted Trivalent Mucosal Vaccine Targeting Key Periodontopathic Bacteria." Vaccines 12, no. 7 (2024): 754. http://dx.doi.org/10.3390/vaccines12070754.

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Periodontal disease (PD) is caused by microbial dysbiosis and accompanying adverse inflammatory responses. Due to its high incidence and association with various systemic diseases, disease-modifying treatments that modulate dysbiosis serve as promising therapeutic approaches. In this study, to simulate the pathophysiological situation, we established a “temporary ligature plus oral infection model” that incorporates a temporary silk ligature and oral infection with a cocktail of live Tannerella forsythia (Tf), Pophyromonas gingivalis (Pg), and Fusobacterium nucleatum (Fn) in mice and tested th

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Aziz, Shahid, Faisal Rasheed, Tayyab Saeed Akhter, Rabaab Zahra, and Simone König. "Microbial Proteins in Stomach Biopsies Associated with Gastritis, Ulcer, and Gastric Cancer." Molecules 27, no. 17 (2022): 5410. http://dx.doi.org/10.3390/molecules27175410.

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(1) Background: Gastric cancer (GC) is the fourth leading cause of cancer-related deaths worldwide. Helicobacter pylori infection is a major risk factor, but other microbial species may also be involved. In the context of an earlier proteomics study of serum and biopsies of patients with gastroduodenal diseases, we explored here a simplified microbiome in these biopsies (H. pylori, Acinetobacter baumannii, Escherichia coli, Fusobacterium nucleatum, Bacteroides fragilis) on the protein level. (2) Methods: A cohort of 75 patients was divided into groups with respect to the findings of the normal

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Taubman, Martin A., Xiaozhe Han, Karen B. LaRosa, Sigmund S. Socransky, and Daniel J. Smith. "Periodontal Bacterial DNA Suppresses the Immune Response to Mutans Streptococcal Glucosyltransferase." Infection and Immunity 75, no. 8 (2007): 4088–96. http://dx.doi.org/10.1128/iai.00623-07.

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ABSTRACT Certain CpG motifs found in bacterial DNA enhance immune responses through Toll-like receptor 9 (TLR-9) and may also demonstrate adjuvant properties. Our objective was to determine if DNA from bacteria associated with periodontal disease could affect the immune response to other bacterial antigens in the oral cavity. Streptococcus sobrinus glucosyltransferase (GTF), an enzyme involved in dental caries pathogenesis, was used as a test antigen. Rowett rats were injected with aluminum hydroxide (alum) with buffer, alum-GTF, or alum-GTF together with either Escherichia coli DNA, Fusobacte

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Khan, Saadullah Jan. "Comparative In Silico Investigation of Biofilm vs Planktonic State Protein Expression in Fusobacterium nucleatum." Academia Letters, May 13, 2022. http://dx.doi.org/10.20935/al5312.

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