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Dissertations / Theses on the topic 'Protein S'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Hon, Jiří. "Vyhledávání příbuzných proteinů s modifikovanou funkcí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234914.

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Protein engineering is a young dynamic discipline with great amount of potential practical applications. However, its success is primarily based on perfect knowledge and usage of all existing information about protein function and structure. To achieve that, protein engineering is supported by plenty of bioinformatic tools and analysis. The goal of this project is to create a new tool for protein engineering that would enable researchers to identificate related proteins with modified function in still growing biological databases. The tool is designed as an automated workflow of existing bioin

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2

He, Xuhua. "Vitamin K-dependent anticoagulant protein S biochemical and histochemical studies /." Lund : Dept. of Clinical Chemistry, Wallenberg Laboratory, University of Lund, University Hospital MAS, 1994. http://catalog.hathitrust.org/api/volumes/oclc/39693810.html.

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3

Radu, Claudia Maria. "Study of the origin of platelets coagulation protein S by human megakaryocyte cultures and characterization of platelets protein S in patients with inherited protein S deficiency." Doctoral thesis, Università degli studi di Padova, 2009. http://hdl.handle.net/11577/3426476.

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Protein S (PS) is a vitamin K dependent plasma glycoprotein with multiple functions in coagulation, inflammation and apoptosis. The molecular weight of PS is approximately 70 kDa and its concentration in plasma is about 25 mg/L. In human plasma 40% of PS circulates as free form and the remaining 60% is complexes with complement C4b-binding protein, a component of the complement system. PS circulating in plasma is mainly derived from liver synthesis but, in addition, endothelial cells, testicular Leydig cells and a megakaryocytic cell line (MEG 01) can synthesize PS. Platelet contain PS, but wh

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4

Wardlaw, Christopher. "Protein-protein interactions underlying damage checkpoint activation in S. pombe." Thesis, University of Sussex, 2014. http://sro.sussex.ac.uk/id/eprint/48121/.

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DNA damage can lead to the accumulation of mutations and diseases such as cancer. It is therefore integral for cells to identify this damaged DNA and promote its repair. To carry out this function eukaryotic cells have evolved signal transduction pathways known as the DNA structure checkpoints. Much of the molecular mechanism underlying these pathways is still far from understood. The work in this thesis uses the model organism Schizosaccharomyces pombe to investigate these mechanisms, with a particular focus on the TopBP1 homolog Rad4. TopBP1 plays an essential scaffolding role in the initiat

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5

Simmonds, Rachel Elizabeth. "Protein S deficiency and familial thrombophilia." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267993.

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6

Hamel, Laura Dawn. "Targeting Autopalmitoylation to Modulate Protein S-Palmitoylation." Scholar Commons, 2015. http://scholarcommons.usf.edu/etd/5960.

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Palmitoylation refers to the covalent attachment of fatty acids, such as palmitate, onto the cysteine residues of proteins. This process may subsequently alter their localization and function. Nearly all of the enzymes that catalyze palmitoylation, zDHHC protein acyl transferases (PATs), are implicated in neurological disorders, infectious diseases, and cancer in humans. Of particular interest to those who study palmitoylation are Ras family GTPas and zDHHC9-GCP16, the zDHHC PAT that palmitoylates Ras proteins. Erf2-Erf4 is the zDHHC PAT that palmitoylates Ras proteins in Saccharomyces cerevis

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7

Hughes, Qunitin William. "Hormonal regulation of the anticoagulant Protein S." University of Western Australia. School of Surgery and Pathology, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0247.

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[Truncated abstract] Every year thousands of individuals suffer from thrombotic related complications that in some cases can be fatal and every year millions of women take some form of hormonal contraceptive. In some cases, there is a cause and effect relationship between the two as users of the combined oral contraceptive pill have an increased risk of developing a thrombotic event. Increased circulating levels of oestrogen cause a prothrombotic shift in the coagulation cascade resulting from upregulation of several procoagulant proteins and a decrease of key anticoagulant proteins. One of th

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8

Zheng, Bin. "RGS proteins : bridging the "GAP"s between G protein signaling and membrane trafficking /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2002. http://wwwlib.umi.com/cr/ucsd/fullcit?p3059905.

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9

Badgandi, Hemant B. "Biology Facilitated by Heme Proteins as Seen in Cimex Nitrophorin and Ecdysone Inducible Protein 75." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/196147.

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This dissertation is a study in how heme facilitates biology using two heme proteins as examples. I write about my mechanistic studies on Cimex nitrophorin and preliminary studies on Ecdysone inducible protein 75, respectively. Nitrophorins are salivary heme proteins used by bloodfeeding insects to deliver NO to the victim, leading to vasodilation and antihemostasis. The bedbug nitrophorin cNP, a thiolate heme protein accomplishes this via an unusual heme-assisted S-nitrosation reaction, requiring proximal ligand cleavage. This dissertation explores this mechanism through mutational, cryst

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10

Rezende, Suely Meireles. "The molecular basis of hereditary protein S deficiency." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289821.

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11

Grundy, Nicholas Matthew. "Protein S-thiolation and oxidative stress in plants." Thesis, Durham University, 2002. http://etheses.dur.ac.uk/3950/.

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The tripeptides glutathione (GSH; γglutamyl-cysteinyl-glycine) and homoglutathione (hGSH; γglutamyl-cysteinyl-β-alanine) are abundant cytosolic tripeptides in legumes. The reactive cysteinyl sulphydryl group enables GSH or hGSH to act as the major cellular redox buffer through the formation of disulphides with other GSH/hGSH molecules. GSH can also form disulphides with cysteinyl groups within proteins, which is termed 5-thiolation, a reversible modification, protecting proteins from irreversible inactivation of thiol residues, as well as being important in regulating protein activity. Followi

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12

Libkind, Marianna. "SiaA: A Heme Protein." Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/chemistry_hontheses/2.

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The protein SiaA (Streptococcal iron acquisition) is involved in heme uptake in the bacterium Streptococcus pyogenes. It is difficult to obtain this protein in its fully holo form (completely loaded with heme). To increase the concentration of heme in the growing cell, we added ä-aminolevulinic acid (ALA) and ferrous sulfate (FeSO4), precursors of heme, to the growth media. Neither increasing the concentration of heme in vivo, nor growth at lower temperature for longer times, increased the production of holoprotein. The classical method of measuring the concentration of heme in a newly discove

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13

Christ, Stefan [Verfasser], and Ulrich [Akademischer Betreuer] Mühlenhoff. "Analyse von posttranslationalen Modifikationen an Fe/S-Proteinen und Protein-Protein-Interaktionen zwischen Fe/S-Assemblierungsfaktoren in Mitochondrien von S. cerevisiae / Stefan Christ. Betreuer: Ulrich Mühlenhoff." Marburg : Philipps-Universität Marburg, 2016. http://d-nb.info/1099594308/34.

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14

Turdzeladze, Tamta [Verfasser], and A. S. [Akademischer Betreuer] Ulrich. "Protein-Protein-Wechselwirkungen des bakteriellen mechanosensitiven Kanals MscL / Tamta Turdzeladze. Betreuer: A. S. Ulrich." Karlsruhe : KIT-Bibliothek, 2011. http://d-nb.info/1019361956/34.

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15

Bhaduri, Samyabrata. "Regulation of CDK1 Activity during the G1/S Transition in S. cerevisiae through Specific Cyclin-Substrate Docking: A Dissertation." eScholarship@UMMS, 2014. http://escholarship.umassmed.edu/gsbs_diss/871.

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Several cell cycle events require specific forms of the cyclin-CDK complexes. It has been known for some time that cyclins not only contribute by activating the CDK but also by choosing substrates and/or specifying the location of the CDK holoenzyme. There are several examples of B-type cyclins identifying certain peptide motifs in their specific substrates through a conserved region in their structure. Such interactions were not known for the G1 class of cyclins, which are instrumental in helping the cell decide whether or not to commit to a new cell cycle, a function that is non-redundant wi

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16

Schwarz, Mathias. "Protein S-100B, diagnostischer und prognostischer Faktor nach Schädelhirntrauma." [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-55852.

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17

Li, Yaxiao. "Diverse roles of protein S-acyl transferases in Arabidopsis thaliana." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.715280.

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S-acylation, commonly known as S-palmitoylation, is a reversible posttranslational lipid modification in which fatty acid, usually palmitic acid, covalently attaches to specific cysteine residues of proteins via thioester bonds. Palmitoylation enhances the hydrophobicity of proteins and contributes to their membrane association. It plays roles in protein trafficking, signalling, protein-protein interaction, protein stability and other important cellular functions. A family of Protein S-acyl Transferases (PATs) is responsible for this reaction. PATs are multi-pass transmembrane proteins that po

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18

Arnljots, Björn. "On prevention of microarterial thrombosis role of protein C and protein S and thrombin inhibition /." Lund : Dept. of Plastic and Reconstructive Surgery, Malmö University Hospital, University of Lund, 1995. http://catalog.hathitrust.org/api/volumes/oclc/39055869.html.

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19

The, Juliana. "Structural Studies of Thioredoxin S-nitrosation and Detection of Protein S-nitrosothiols by Phosphine Derivatization." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/312667.

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S-nitrosylation (or S-nitrosation) has emerged as an important pathway of non-classical nitric oxide signaling. This post-translational modification involves the transfer of a nitroso group onto a cysteine residue and has been shown to regulate protein function. However, very little is known about the mechanism and structure-dependent factors of the modification. Understanding of S-nitrosothiol chemistry has lagged behind that for the classical nitric oxide signaling pathway due to challenges and limitations of current detection methods of S-nitrosothiols. The S-N bond is typically labile and

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20

Cunha, Hilda Helena Souza da. "Protein?ria e ?cido ?rico s?rico maternos em pacientes com s?ndrome de HELLP." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2012. http://tede2.pucrs.br/tede2/handle/tede/1708.

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Made available in DSpace on 2015-04-14T13:35:36Z (GMT). No. of bitstreams: 1 443764.pdf: 1879751 bytes, checksum: a84345f48429752c882a6b33df97af04 (MD5) Previous issue date: 2012-08-28<br>Objective: To evaluate the association of maternal serum uric acid (UA) and proteinuria with clinical and demographic data of pregnant women with preeclampsia syndrome (PES) complicated by HELLP syndrome. Methods: One hundred and nine pregnant women were divided into two groups: group 1 - HELLP pregnant women with PES complicated by HELLP syndrome (n=64); group 2 PES pregnant women with PES but no HEL

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21

Boyes, Barry Edward. "An immunochemical and immunocytochemical study of the S-100b protein." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/24485.

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This thesis describes an immunochemical and immunocytochemical study of the bovine brain S-lOOb protein. The two major forms of the S-100 isoproteins (S-lOOa and S-lOOb) were purified to apparent homogeneity from bovine brain. A polyclonal rabbit antiserum to the S-lOOb protein was prepared. The antiserum was characterized by solid phase immunochemical methods. The S-lOOb derived antiserum displayed a high degree of specificity for S-lOOb, but also crossreacted with the purified S-lOOa protein. The characteristics of the immunochemical reactivity of the antiserum towards these two isoproteins

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22

Norte, Valia. "Identification of human lactoferrin binding protein(s) in Helicobacter pylori." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285774.

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23

Kinns, H. J. "Mapping the molecular structure of the S-layer protein SbsB." Thesis, University College London (University of London), 2010. http://discovery.ucl.ac.uk/19488/.

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Surface layer (S-layer) proteins form 2-dimensional crystalline structures at the cell surface of most eubacteria and all archaea. S-layers are generally composed of a single self-assembling (glyco)protein species and can fulfil a number of roles including shape maintenance, exo-enzyme adhesion, and virulence promotion. Engineered S-layers carrying functional inserts at high density also show potential in nanotechnology. However, there is currently no complete structure at atomic resolution available for any S-layer protein. This work is engaged in the (i) structural elucidation of the SbsB S-

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24

Andersson, Helena. "Characterisation of the molecular basis of protein S anticoagulant function." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/6130.

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Protein S has an established role in the protein C anticoagulant pathway as a cofactor for anticoagulant protein C (APC) and has also recently been shown to serve as a cofactor enhancing the anticoagulant activity of tissue factor pathway inhibitor (TFPI). Despite its physiological role and clinical importance, the molecular bases of its functions are not fully understood. The aim of my thesis was to clarify the molecular mechanisms involved in the protein S interaction with APC and TFPI. More than 30 point or composite protein S variants were constructed and analysed during this project. Thes

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25

Eifart, Patricia. "Visualisierung und Charakterisierung der S-Protein vermittelten Fusion von Coronaviren." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2008. http://dx.doi.org/10.18452/15733.

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Die Fusionsreaktion des Coronavirus MHV wird vom S-Protein vermittelt. In der vorliegenden Arbeit wurde der Eintrittsweg von MHV-A59 in Mauszellen untersucht. Die Infektivität kann durch lysosomotrope Substanzen und Inhibitoren der Clathrin-abhängigen Endozytose gehemmt werden. Der Eintritt von MHV-A59 in Mauszellen erfolgt über die Clathrin-abhängige Endozytose und setzt die anschließende Fusion der viralen und zellulären Membran bei niedrigem pH-Wert voraus. Fluoreszenzmikroskopische Studien zur Interaktion fluoreszenzmarkierter MHV-A59 Partikel mit Mauszellen bestätigen, dass MHV-A59 über E

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26

DeMoor, Janice Marie Carleton University Dissertation Biology. "Expression of an Arabidopsis Thaliana 2S Albumin storage protein gene in transgenic Nicotiana Tabacum and Brassica Napus." Ottawa, 1992.

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27

Li, Hui. "Computational studies of protein pK(a)s and metalloprotein reduction potentials." Diss., University of Iowa, 2004. http://ir.uiowa.edu/etd/121.

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28

Molik, Sabine. "Das plastidäre Rieske Fe/S-Protein Analyse des Transport- und Assemblierungsprozesses /." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975694057.

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29

Salter, A. Hugh. "Genetics and biogensis of the pea chloroplast Rieske Fe-S protein." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335798.

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30

Hall, Adrian John. "Molecular analysis of the factor IX and protein S gene promoters." Thesis, University of Sheffield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364379.

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31

Frost, Victoria. "Regulation of protein synthesis by the rapamycin sensitive signalling pathways(s)." Thesis, University of Sussex, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360493.

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32

Lin, Su-Jiun. "Structure/function analysis of the essential protein Rad4TopBP1 in S. pombe." Thesis, University of Sussex, 2011. http://sro.sussex.ac.uk/id/eprint/6326/.

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Rad4TopBP1, a multiple BRCT domain protein, is essential for initiation of replication and participates in checkpoint responses following genotoxic treatment during the S and G2 phases of the cell cycle. Rad4 interacts with several proteins that are involved in initiation of DNA replication (i.e. Sld2) or activation of Chk1 and/or Cds1 checkpoint kinases (i.e. Rad9, Crb2, and Rad3). However, it remained unclear how Rad4 and its homologues are regulated to coordinate these diverse functions. This PhD project presents a comprehensive structure/function analysis of the fission yeast Rad4TopBP1. I

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33

Kuroda, Shun'ichi. "STUDIES ON HEPATITIS B VIRUS ENVELOPE PROTEIN CONTAINING PRE-S PEPTIDE." Kyoto University, 1992. http://hdl.handle.net/2433/168904.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである<br>Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第8019号<br>論農博第1796号<br>新制||農||640(附属図書館)<br>学位論文||H4||N2518(農学部図書室)<br>UT51-92-U255<br>(主査)教授左右田健次, 教授駒野徹, 教授清水昌<br>学位規則第4条第2項該当

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34

Janes, Simon. "Novel isoforms & functions of the S. pombe Rad9 checkpoint protein." Thesis, Bangor University, 2012. https://research.bangor.ac.uk/portal/en/theses/novel-isoforms-and-functions-of-the-spombe-rad9-checkpoint-protein(a4fe3b1b-1fbc-476a-8e45-a12bff6a7096).html.

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Cancer is of paramount medical concern as an increasingly major contributor of disease-related fatalities of significant prevalence - particularly in the context of current statisticaLlstochastical epidemiological studies which predict that one in three people will contract cancer at some stage of their lives, whilst one in four of these patients will die as a consequence of their particular neoplastic-associated condition. The human Rad9 protein exists in two full-length isoforms (termed Rad9A and Rad9B) whose respective differentially-elevated levels and related expression profiles are disti

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35

Xu, Xiaojun [Verfasser], and A. S. [Akademischer Betreuer] Ulrich. "Protein-protein interactions in oligomers studied by solid-state NMR in biomembranes / Xiaojun Xu. Betreuer: A. S. Ulrich." Karlsruhe : KIT-Bibliothek, 2016. http://d-nb.info/1108453198/34.

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Niemand, Jandeli. "A phage display study of interacting peptide binding partners of malarial S-Adenosylmethionine decarboxylase/Ornithine decarboxylase." Diss., University of Pretoria, 2007. http://hdl.handle.net/2263/24105.

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Due to the increasing resistance against the currently used antimalarial drugs, novel chemotherapeutic agents that target new metabolic pathways for the treatment of malarial infections are urgently needed. One approach to the drug discovery process is to use interaction analysis to find proteins that are involved in a specific metabolic pathway that has been identified as a drug target. Protein-protein interactions in such a pathway can be preferential targets since a) there is often greater structural variability in protein-protein interfaces, which can lead to more effective differentiation

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37

Nookala, Ravi. "Mechanism(s) of resistance of Helicobacter pylori towards metronidazole." Thesis, University of East London, 2000. http://roar.uel.ac.uk/3868/.

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Metronidazole is an essential component of the triple therapy regimen against Helicobacter pylori infection. The development of resistance towards metronidazole results in failure to eradicate H. pylori completely. The main aim of the investigation was to understand further the mechanism(s) of resistance in H. pylori. The investigation focussed upon studying the role and function of NADH oxidase in metronidazole resistance. The NADH oxidase levels were shown to be significantly higher in metronidazole susceptible strains than in resistant strains. The purification and characterisation of the e

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38

Varga, Melinda. "Self-assembly of the S-layer protein of Sporosarcina ureae ATCC 13881." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-65141.

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Increasing the integration density of electron device components will necessitate the use of new nanofabrication paradigms that complement and extend existing technologies. One potential approach to overcome the current limitations of electron-beam lithography may involve the use of hybrid systems, in which existing lithographic techniques are coupled with “bottom up” approaches such as supramolecular self-assembly. In this respect, biological systems offer some unique possibilities as they combine both self-organization and spatial patterning at the nanometer length scale. In particular, Surf

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Hamnell-Pamment, Ylva. "Novel methods for the identification of cellular S-glutathionylated proteins and sites of glutathionedependent modification using affinity chromatography and proteomic analyses /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-248-9/.

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Marenna, Alessandra. "Staphylococcus aureus protein S1, an RNA chaperone involved in translation initiation and sRNA regulation." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ080/document.

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Bien que l'initiation de la traduction soit un processus conservé entre les bactéries, nous avons montré que le mécanisme par lequel les ARNm structurés sont reconnus et adaptés sur le ribosome diffère chez Staphylococcus aureus, un micro-organisme avec un bas taux de G+C et chez Escherichia coli. Une particularité du ribosome de S. aureus est l'absence de la protéine ribosomale S1, qui non seulement est plus courte que celle de E. coli mais qui possède également une organisation distincte des domaines. Mes expériences suggèrent que la protéine S1 (SauS1) favorise spécifiquement l'initiation d

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Ryzhkov, Pavel. "Bioengineering of S-layers: molecular characterization of the novel S-layer gene sslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1204126129404-82781.

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S-layer proteins of S. ureae ATCC 13881 form on the cell surface an S-layer lattice with p4 square type symmetry and a period of about 13.5 nm. These lattices were shown to be the excellent nanotemplates for deposition of regular metal clusters. The synthesis of the S. ureae S-layer protein is highly efficient, the protein accounts for approximately 10-15 % of the total cell protein content, judged by the SDS-PAGE results. Besides, the S-layer protein production is tightly regulated, since only negligible amounts of S-layer proteins are observed in the medium at different cell growth phases. A

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Yasmin, Lubna. "Exoenzyme S of Pseudomonas aeruginosa : cellular targets and interaction with 14-3-3." Doctoral thesis, Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1411.

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Ahmad, Margaret. "Characterization of the CAN1 gene and its product in S. cerevisiae." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=72090.

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This work describes the characterization of the CAN1 gene, thought to encode the arginine permease of yeast. I have identified the RNA transcript of this gene and obtained the DNA sequence, which specifies a highly hydrophobic protein with multiple potential membrane-spanning domains. I use a gene fusion approach to identify sequences within the CAN1 protein that can translocate adjacent sequences across the E.R. membrane in vitro and extend these observations by finding that the topology of E.R. insertion is conserved up to the plasma membrane in vivo. Using a series of CAN1 gene fusions to t

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Meyer, Lauren Francis. "Meiosis-Specific Regulation of Centromeric Chromatin and Chromosome Segregation by a Transposase-Derived Protein." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:107029.

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Thesis advisor: Charles Hoffman<br>Faithful chromosome segregation is necessary for the successful completion of mitosis and meiosis. The centromere is the site of kinetochore and microtubule attachment during chromosome segregation, and it is critical that the centromere is properly formed and maintained. Many proteins contribute to centromere formation, and this process has been extensively studied during the mitotic cell cycle. However, the roles of the centromere and its associated proteins during meiosis and their contribution to the fidelity of chromosome segregation process are not as w

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45

Olech, Tony. "Neuropsychological functioning and protein S-100ℓ levels before and after carotid endarterectomy /". Adelaide, 2000. http://web4.library.adelaide.edu.au/theses/09AR.PS/09ar.pso449.pdf.

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Hockey, Verity Irena. "Characterising the molecular interaction between tissue factor pathway inhibitor and protein S." Thesis, Imperial College London, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.530472.

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47

Rakestraw, James A. "A directed evolution approach to engineering recombinant protein production in S. cerevisiae." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/38242.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006.<br>Vita.<br>Includes bibliographical references.<br>The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. coli and bovines, the single-celled organism S. cerevisiae has emerged as a well-qualified candidate due to its approachable genetic and fermentation att

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Chouchani, Edward Thomas. "Mitochondrial protein S-nitrosation in the living heart during ischaemia-reperfusion injury." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607866.

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49

Bielskienė, Kristina. "Analysis of the barley (Hordeum vulgare) tightly bound DNA-protein complexes." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20091202_111955-77123.

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Abstract:

Despite a great deal of research, the functional significance of tightly bound DNA-protein complexes is not yet clear, therefore these complexes are perfect object for pioneering research. Very little is known about plant TBP-DNA complexes. In this work we investigated barley TBP-DNA complexes from different organs (first leaves, roots and coleoptiles) at different developmental stages. We characterized individual components of tightly bound DNA-proteins complexes: polypeptides (TBP) and DNA. We isolated and characterized TBP proteins from barley first leaves, roots and coleoptiles of differen

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Hildreth, Sherry Boston. "Investigation of Protein-Protein Interactions among Nicotine Biosynthetic Enzymes and Characterization of a Nicotine Transporter." Diss., Virginia Tech, 2003. http://hdl.handle.net/10919/29698.

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Abstract:

Alkaloids are a class of plant secondary metabolites produced in about 20% of plant families. Domesticated tobacco, Nicotiana tabacum produces nicotine as the predominant alkaloid. The biosynthesis of nicotine occurs exclusively in the roots of tobacco, yet accumulates in the leaves of tobacco where it is acts as a defense compound to deter insect herbivory. The research detailed in this dissertation addresses two aspects of nicotine physiology in tobacco: 1) an investigation of hypothesized protein-protein interactions among nicotine biosynthetic enzymes and 2) the characterization of a novel

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