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Journal articles on the topic 'Protein-tyrosine kinase Testis. Rats'

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Published: 4 June 2021

Last updated: 6 February 2022

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1

Zhang, Jiayi, Ching-hang Wong, Weiliang Xia та ін. "Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-β-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model". Endocrinology 146, № 3 (2005): 1268–84. http://dx.doi.org/10.1210/en.2004-1194.

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Using a well characterized model of cell-cell actin-based adherens junction (AJ) disruption by suppressing the intratesticular testosterone level in adult rats with testosterone-estradiol implants, we have confirmed earlier findings that Sertoli-germ cell AJ dynamics are regulated by the activation of kinases via putative signaling pathways but with some unexpected findings as follows. First, the loss of germ cells from the seminiferous epithelium during androgen suppression was associated with a surge in myotubularin-related protein 2 (MTMR2, a lipid phosphatase, in which adult MTMR2−/− mice

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Girke, Vera, Josephine Tabea Tauer, Ingmar Glauche, Lorenz Hofbauer, and Meinolf Suttorp. "Impact of Long-Term Tyrosine Kinase Inhibitor Exposure on Spermatogenesis in Juvenile Rats." Blood 128, no. 22 (2016): 1884. http://dx.doi.org/10.1182/blood.v128.22.1884.1884.

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Abstract Background: Pediatric patients with chronic myeloid leukemia (CML) are exposed to off-target side effects from long-term treatment with tyrosine kinase inhibitors (TKIs) which have been observed with increasing watchfulness in the last years (Hijiya N, et al. BLOOD 127:392, 2016). TKIs inhibit c-kit and platelet-derived growth factor receptors (PDGF-R alpha/beta) which are known to regulate spermatogenesis (Zhang M, et al. SCI REP 4:5936, 2014). The influence of TKIs on spermatogenesis in pediatric patients with CML is not fully understood yet (Samis J, et al. PEDIATR BLOOD CANCER 63:

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Tang, Elizabeth I., Will M. Lee, and C. Yan Cheng. "Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis." Endocrinology 2016, no. 1 (2016): 47–62. http://dx.doi.org/10.1210/en.2015-1962.

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Abstract Germ cell transport across the seminiferous epithelium during spermatogenesis requires the intricate coordination of cell junctions, signaling proteins, and both actin- and microtubule (MT)-based cytoskeletons. Although the involvement of cytoskeletons in germ cell transport has been suggested, the precise mechanism(s) remains elusive. Based on growing evidencethat actin and MT interactions underlie fundamental cellular processes, such as cell motility, it is unlikely that actin- and MT-based cytoskeletons work independently to regulate germ cell transport in the testis. Using rats tr

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Toyoshima, Yuka, Reiko Tokita, Yoichiro Ohne, et al. "Dietary protein deprivation upregulates insulin signaling and inhibits gluconeogenesis in rat liver." Journal of Molecular Endocrinology 45, no. 5 (2010): 329–40. http://dx.doi.org/10.1677/jme-10-0102.

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This study was undertaken to elucidate the effects of dietary protein deprivation on glucose metabolism and hepatic insulin signaling in rats. The results of glucose and pyruvate tolerance tests in rats fed with a 12% casein diet (12C) and a protein-free diet (PF) indicated that protein deprivation enhanced clearance of blood glucose and suppressed gluconeogenesis. Correspondingly, the mRNA level of hepatic phosphoenolpyruvate carboxykinase, a key gluconeogenic enzyme, was suppressed by dietary protein deprivation. In PF-fed rats, total tyrosine phosphorylation of insulin receptor (IR) in the

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El Morsy, EM, and MAE Ahmed. "Protective effects of lycopene on hippocampal neurotoxicity and memory impairment induced by bisphenol A in rats." Human & Experimental Toxicology 39, no. 8 (2020): 1066–78. http://dx.doi.org/10.1177/0960327120909882.

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Bisphenol A (BPA) is used to produce polycarbonate plastic and epoxy resins which are used in many consumer products. Most people encounter BPA in their daily routines. However, it has been heavily reported that BPA has a neurotoxic effect. The present study aimed to investigate the effect of lycopene on cognitive deficits induced by a high dose of BPA focusing on mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway, oxidative stress, apoptosis, and memory retrieval in adult male rats. Therefore, 72 rats were divided into four groups: control group, BPA g

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Cancilla, B., A. Davies, M. Ford-Perriss, and GP Risbridger. "Discrete cell- and stage-specific localisation of fibroblast growth factors and receptor expression during testis development." Journal of Endocrinology 164, no. 2 (2000): 149–59. http://dx.doi.org/10.1677/joe.0.1640149.

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Fibroblast growth factors (FGFs) are a family of heparin binding proteins involved in many biological processes. These growth factors act through tyrosine kinase receptors (FGFRs); we have previously used immunohistochemistry to study FGFRs-1-4 in foetal, immature and adult rat testes, and found a discrete cell- and stage-specific localisation. Alternative mRNA splicing of FGFRs-1-3 leads to functional variants (IIIb and IIIc) with distinct ligand binding affinities, therefore we have identified the specific expression of functional FGFR variants and the expression and localisation of FGF liga

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Zamoner, Ariane, Kátia Padilha Barreto, Danilo Wilhelm Filho, et al. "Propylthiouracil-induced congenital hypothyroidism upregulates vimentin phosphorylation and depletes antioxidant defenses in immature rat testis." Journal of Molecular Endocrinology 40, no. 3 (2008): 125–35. http://dx.doi.org/10.1677/jme-07-0089.

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Congenital hypothyroidism was induced in rats by adding 0.05% 6-propyl-2-thiouracil in the drinking water from day 9 of gestation, and continually up to postnatal day 15. Structural alterations observed by light microscopy of seminiferous tubules and by transmission electron microscopy of Sertoli cells of treated animals were consistent with hypothyroid condition. Hypothyroidism was also associated with high phospho-p38 mitogen-activated protein kinase and decreased phospho-extracellular signal-regulated kinase 1/2 levels. Furthermore, the phosphorylation and the immunoreactivity of cytoskelet

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Bouraïma-Lelong, Hélène, Marion Vanneste, Christelle Delalande, Leïla Zanatta, Slawomir Wolczynski, and Serge Carreau. "Aromatase gene expression in immature rat Sertoli cells: age-related changes in the FSH signalling pathway." Reproduction, Fertility and Development 22, no. 3 (2010): 508. http://dx.doi.org/10.1071/rd09168.

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Aromatase, the enzyme responsible for the transformation of androgens into oestrogens, is encoded by the cyp19 gene expressed in the testis. The aim of the present study was to analyse the evolution of aromatase gene expression under FSH control in rat Sertoli cells between 10 and 30 days post partum, corresponding to the end of the proliferative period of Sertoli cells, establishment of the blood–testis barrier and acquisition of the mature phenotype. The maximum stimulatory effect of FSH on aromatase gene expression was obtained in 20-day-old rat Sertoli cells, compared with cells from 10- a

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Catizone, A., G. Ricci, J. Del Bravo, and M. Galdieri. "Hepatocyte growth factor modulates in vitro survival and proliferation of germ cells during postnatal testis development." Journal of Endocrinology 189, no. 1 (2006): 137–46. http://dx.doi.org/10.1677/joe.1.06528.

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The hepatocyte growth factor (HGF) is a pleiotropic cytokine that influences mitogenesis, motility and differentiation of many different cell types by its tyrosine kinase receptor c-Met. We previously demonstrated that the c-Met/HGF system is present and functionally active during postnatal testis development. We found also that spermatozoa express c-Met and that HGF has a positive effect on the maintenance of sperm motility. In the present paper, we extend our study on the germ cells at different stages of differentiation during the postnatal development of the testis. We demonstrate that c-m

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Michel, U., J. Esselmann, and E. Nieschlag. "Expression of follistatin messenger ribonucleic acid in Sertoli cell-enriched cultures: regulation by epidermal growth factor and protein kinase C-dependent pathway but not by follicle-stimulating hormone and protein kinase A-dependent pathway." Acta Endocrinologica 129, no. 6 (1993): 525–31. http://dx.doi.org/10.1530/acta.0.1290525.

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In this study the localization and regulation of steady-state follistatin messenger ribonucleic acid (mRNA) levels in testicular cell cultures were examined with a solution-hybridization assay using a specific 32P-labelled cytosolic RNA antisense probe for follistatin and a 35S-labelled cytosolic RNA antisense probe for cyclophilin as internal standard. Testes from immature rats were dispersed with collagenase and fractionated in Sertoli and Leydig cell-enriched cultures. Follistatin mRNA was mainly localized to the Sertoli cell-enriched fraction and the expression of follistatin mRNA could be

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Shaif, Noof, Daehyuk Jang, Donghyun Cho, Sunmi Kim, Dae Seo, and Insop Shim. "The Antidepressant-Like Effect of Lactate in an Animal Model of Menopausal Depression." Biomedicines 6, no. 4 (2018): 108. http://dx.doi.org/10.3390/biomedicines6040108.

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Background: This study aimed to investigate the antidepressant-like effect of lactate and elucidate its mechanisms in ovariectomized rats with repeated stress. Methods: Two experiments were conducted on female rats in which all groups, except normal, were ovariectomized and underwent immobilization for 14 days. Lactate was administered orally (100, 250, and 500 mg/kg) for 14 consecutive days, and the rats’ cutaneous body temperature was measured during the same period. Depression-like behavior in rats was assessed by the tail suspension test (TST) and forced swimming test (FST). Furthermore, e

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Lucas, Thaís F. G., Luciana S. Amaral, Catarina S. Porto та Luis E. M. Quintas. "Na+/K+-ATPase α1 isoform mediates ouabain-induced expression of cyclin D1 and proliferation of rat Sertoli cells". REPRODUCTION 144, № 6 (2012): 737–45. http://dx.doi.org/10.1530/rep-12-0232.

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Novel roles for the interaction of cardiotonic steroids to Na+/K+-ATPase have been established in recent years. The aim of this study was to investigate the intracellular signaling events downstream the action of ouabain on Na+/K+-ATPase in Sertoli cell obtained from immature rats. Treatment of Sertoli cells with ouabain (1 μM) induced a rapid and transient increase in the extracellular signal-regulated kinase (ERK1/2 or MAPK3/1) and phosphatidylinositol 3-kinase (PI3K)/serine–threonine protein kinase (AKT) phosphorylation. Also, ouabain upregulated the expression of cyclin D1 and incorporatio

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Haller, Meade, Qianxing Mo, Akira Imamoto, and Dolores J. Lamb. "Murine model indicates 22q11.2 signaling adaptor CRKL is a dosage-sensitive regulator of genitourinary development." Proceedings of the National Academy of Sciences 114, no. 19 (2017): 4981–86. http://dx.doi.org/10.1073/pnas.1619523114.

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The spectrum of congenital anomalies affecting either the upper tract (kidneys and ureters) or lower tract (reproductive organs) of the genitourinary (GU) system are fundamentally linked by the developmental origin of multiple GU tissues, including the kidneys, gonads, and reproductive ductal systems: the intermediate mesoderm. Although ∼31% of DiGeorge/del22q11.2 syndrome patients exhibit GU defects, little focus has been placed on the molecular etiology of GU defects in this syndrome. Among del22q11.2 patients exhibiting GU anomalies, we have mapped the smallest relevant region to only five

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Li, Hua, Vassilios Papadopoulos, Branislav Vidic, Martin Dym, and Martine Culty. "Regulation of Rat Testis Gonocyte Proliferation by Platelet-Derived Growth Factor and Estradiol: Identification of Signaling Mechanisms Involved*." Endocrinology 138, no. 3 (1997): 1289–98. http://dx.doi.org/10.1210/endo.138.3.5021.

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Abstract To determine what factors regulate gonocyte proliferation in newborn rats, we first examined the expression of several signal transduction molecules by immunocytochemistry in 3-day-old rat testis sections. We found that gonocytes specifically expressed the ι and ζ isoforms of protein kinase (PK) C (PKC) and the phosphatidylinositol 3-kinase (PI 3-K). Because both the ζPKC and PI 3-K have been shown to play a role in platelet-derived growth factor (PDGF)-induced cell proliferation, we examined the effects of PDGF on gonocytes. For this, we developed a method to obtain highly purified a

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Nicholls, P. K., P. G. Stanton, K. L. Walton, R. I. McLachlan, L. O'Donnell, and C. A. Harrison. "148. HORMONALLY REGULATED miRNAS TARGET THE TUBULOBULBAR COMPLEX IN THE TESTIS." Reproduction, Fertility and Development 22, no. 9 (2010): 66. http://dx.doi.org/10.1071/srb10abs148.

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Spermatogenesis is absolutely dependent on follicle stimulating hormone (FSH) and androgens; acute suppression of these hormones inhibits germ cell development and thus sperm production. The removal of intercellular junctions and release of spermatids by the Sertoli cell, a process known as spermiation, is particularly sensitive to acute hormone suppression(1). To define the molecular mechanisms that mediate FSH and androgen effects in the testis, we investigated the expression and hormonal regulation of micro-RNAs (miRNA), small non-coding RNAs that regulate protein translation and modify cel

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Zanatta, Leila, Hélène Bouraïma-Lelong, Christelle Delalande, Fátima R. M. B. Silva та Serge Carreau. "Regulation of aromatase expression by 1α,25(OH)2 vitamin D3 in rat testicular cells". Reproduction, Fertility and Development 23, № 5 (2011): 725. http://dx.doi.org/10.1071/rd10163.

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It is well known that the vitamin D endocrine system is involved in physiological and biochemical events in numerous tissues, especially gut, bone and kidney but also testis. Therefore, in this study the effect and mechanisms of action of 1α,25(OH)2 vitamin D3 (1,25D) on aromatase gene expression in immature rat Sertoli cells were evaluated. Vitamin D receptor transcripts were present in immature Sertoli cells as well as in adult testicular germ cells and somatic cells. The treatment of immature Sertoli cells with 100 nM 1,25D increased the amount of aromatase transcript, mainly in 30-day-old

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SASAKI, Takashi, Jun KOTERA, and Kenji OMORI. "Novel alternative splice variants of rat phosphodiesterase 7B showing unique tissue-specific expression and phosphorylation." Biochemical Journal 361, no. 2 (2002): 211–20. http://dx.doi.org/10.1042/bj3610211.

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cDNA species coding for novel variants of cyclic-AMP-specific phosphodiesterases (PDEs), namely the PDE7B family, were isolated from rats and characterized. Rat PDE7B1 (RNPDE7B1) was composed of 446 amino acid residues. Rat PDE7B2 (RNPDE7B2) and PDE7B3 (RNPDE7B3), which possessed unique N-terminal sequences, consisted of 359 and 459 residues respectively. Northern hybridization analysis showed that rat PDE7B transcripts were particularly abundant in the striatum and testis. PCR analyses revealed that rat PDE7B2 transcripts were restricted to the testis and that low levels of PDE7B3 transcripts

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Colón, Eugenia, Farasat Zaman, Magnus Axelson, et al. "Insulin-Like Growth Factor-I Is an Important Antiapoptotic Factor for Rat Leydig Cells during Postnatal Development." Endocrinology 148, no. 1 (2007): 128–39. http://dx.doi.org/10.1210/en.2006-0835.

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The present investigation examines the influence of IGF-I and the role of IGF-I receptor (IGF-IR) in the apoptosis/survival of Leydig cells. Immunohistochemical analysis of the rat testis at different ages revealed that the level of the phosphorylated IGF-IR increases from birth to d 20 of postnatal life, remaining high in the adult testis. Western blotting revealed that this level is higher in Leydig cells isolated from 40-d-old than from 10- or 60-d-old rats. Application of the terminal deoxyribonucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay revealed that

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Fischman, K., J. C. Edman, G. M. Shackleford, J. A. Turner, W. J. Rutter, and U. Nir. "A murine fer testis-specific transcript (ferT) encodes a truncated Fer protein." Molecular and Cellular Biology 10, no. 1 (1990): 146–53. http://dx.doi.org/10.1128/mcb.10.1.146.

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A cDNA for a potential tyrosine kinase-encoding mRNA was isolated from a mouse testis cDNA library. In a survey of eight mouse tissues, a transcript of 2.4 kilobases restricted to testis tissue was found. The mRNA encodes a 453-amino-acid protein of 51,383 daltons, the smallest tyrosine kinase protein ever described. RNA synthesized from the cDNA template directs the synthesis of a 51,000-Mr protein in a cell-free translation system. The carboxy-terminal 409 amino acids are 98 and 90% identical to the carboxy halves of the rat and human Fer proteins, respectively. This suggests that the cDNA r

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Fischman, K., J. C. Edman, G. M. Shackleford, J. A. Turner, W. J. Rutter, and U. Nir. "A murine fer testis-specific transcript (ferT) encodes a truncated Fer protein." Molecular and Cellular Biology 10, no. 1 (1990): 146–53. http://dx.doi.org/10.1128/mcb.10.1.146-153.1990.

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A cDNA for a potential tyrosine kinase-encoding mRNA was isolated from a mouse testis cDNA library. In a survey of eight mouse tissues, a transcript of 2.4 kilobases restricted to testis tissue was found. The mRNA encodes a 453-amino-acid protein of 51,383 daltons, the smallest tyrosine kinase protein ever described. RNA synthesized from the cDNA template directs the synthesis of a 51,000-Mr protein in a cell-free translation system. The carboxy-terminal 409 amino acids are 98 and 90% identical to the carboxy halves of the rat and human Fer proteins, respectively. This suggests that the cDNA r

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Xu, Qian, Shasha Yang, Fangzhen Wu, et al. "Congrong Shujing Granule-Induced GRP78 Expression Reduced Endoplasmic Reticulum Stress and Neuronal Apoptosis in the Midbrain in a Parkinson’s Disease Rat Model." Evidence-Based Complementary and Alternative Medicine 2020 (September 30, 2020): 1–12. http://dx.doi.org/10.1155/2020/4796236.

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The main pathological changes inherent in Parkinson’s disease (PD) are degeneration and loss of dopamine neurons in the midbrain and formation of Lewy bodies. Many studies have shown that the pathogenesis of PD is closely related to endoplasmic reticulum (ER) oxidative stress. This study combined various traditional Chinese medicines to prepare Congrong Shujing granules (CSGs). The optimal dose combination of the ingredients was identified by experimental intervention in SH-SY5Y cells in vitro. A PD rat model was established by intraperitoneal injection of rotenone sunflower oil emulsion. The

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Miki, Yuka, Lalitha Devi, Yuji Imai, Naojiro Minami, Tsuyoshi Koide, and Sandeep Goel. "Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice." Reproduction, Fertility and Development 32, no. 10 (2020): 893. http://dx.doi.org/10.1071/rd19445.

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The PDZ-binding kinase (PBK) protein is localised exclusively in spermatogenic cells, such as spermatogonia, spermatocytes and round spermatids, of the adult testis. However, its role in male fertility remains unknown. Analysis of adult Pbk-knockout (KO) male mice showed no significant difference in the weight of the testes, epididymis and seminal vesicle compared with adult wild-type (WT) mice. There were no significant differences in testis morphology, tubule diameter and the number of offspring born to females mated with KO or WT male mice. Sperm number, motility and morphology did not diff

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Gu, Rui-Min, Yuan Wei, John R. Falck, U. Murali Krishna, and Wen-Hui Wang. "Effects of protein tyrosine kinase and protein tyrosine phosphatase on apical K+ channels in the TAL." American Journal of Physiology-Cell Physiology 281, no. 4 (2001): C1188—C1195. http://dx.doi.org/10.1152/ajpcell.2001.281.4.c1188.

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We have previously demonstrated that the protein level of c-Src, a nonreceptor type of protein tyrosine kinase (PTK), was higher in the renal medulla from rats on a K-deficient (KD) diet than that in rats on a high-K (HK) diet (Wang WH, Lerea KM, Chan M, and Giebisch G. Am J Physiol Renal Physiol 278: F165–F171, 2000). We have now used the patch-clamp technique to investigate the role of PTK in regulating the apical K channels in the medullary thick ascending limb (mTAL) of the rat kidney. Inhibition of PTK with herbimycin A increased NP o, a product of channel number ( N) and open probability

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Frankel, Mark, Ararat J. Ablooglu, Joseph W. Leone, et al. "Intrasteric Inhibition of ATP Binding Is Not Required To Prevent Unregulated Autophosphorylation or Signaling by the Insulin Receptor." Molecular and Cellular Biology 21, no. 13 (2001): 4197–207. http://dx.doi.org/10.1128/mcb.21.13.4197-4207.2001.

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ABSTRACT Receptor tyrosine kinases may use intrasteric inhibition to suppress autophosphorylation prior to growth factor stimulation. To test this hypothesis we made an Asp1161Ala mutant in the activation loop that relieved intrasteric inhibition of the unphosphorylated insulin receptor (IR) and its recombinant cytoplasmic kinase domain (IRKD) without affecting the activated state. Solution studies with the unphosphorylated mutant IRKD demonstrated conformational changes and greater catalytic efficiency from a 10-fold increase ink cat and a 15-fold-lowerK m ATP althoughK m peptide was unchange

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Majumdar, Adhip P. N., and James R. Goldenring. "Localization and significance of pp55, a gastric mucosal membrane protein with tyrosine kinase activity." American Journal of Physiology-Gastrointestinal and Liver Physiology 274, no. 5 (1998): G863—G870. http://dx.doi.org/10.1152/ajpgi.1998.274.5.g863.

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In Fischer 344 rats, induction of gastric mucosal proliferative activity, whether the result of aging or injury or occurring after administration of epidermal growth factor, gastrin, or bombesin, is associated with a rise in tyrosine kinase activity and tyrosine phosphorylation of several mucosal proteins, including a protein with a molecular mass of 53–55 kDa. We hypothesized that this phosphotyrosine membrane protein (referred to as pp55) may play a role in regulating gastric mucosal cell proliferation and differentiation. Purification and subsequent immunoprecipitation studies now show that

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Ludwig, Lynda M., Dorothee Weihrauch, Judy R. Kersten, Paul S. Pagel, and David C. Warltier. "Protein Kinase C Translocation and Src Protein Tyrosine Kinase Activation Mediate Isoflurane-induced Preconditioning In Vivo." Anesthesiology 100, no. 3 (2004): 532–39. http://dx.doi.org/10.1097/00000542-200403000-00011.

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Background The authors tested the hypotheses that protein kinase C (PKC)-specific isoform translocation and Src protein tyrosine kinase (PTK) activation play important roles in isoflurane-induced preconditioning in vivo. Methods Rats (n = 125) instrumented for measurement of hemodynamics underwent 30 min of coronary artery occlusion followed by 2 h of reperfusion and received 0.9% saline (control); PKC inhibitors chelerythrine (5 mg/kg), rottlerin (0.3 mg/kg), or PKC-epsilonV1-2 peptide (1 mg/kg); PTK inhibitors lavendustin A (1 mg/kg) or 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]py

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Wang, Wenhui, Kenneth M. Lerea, Mary Chan, and Gerhard Giebisch. "Protein tyrosine kinase regulates the number of renal secretory K channels." American Journal of Physiology-Renal Physiology 278, no. 1 (2000): F165—F171. http://dx.doi.org/10.1152/ajprenal.2000.278.1.f165.

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The apical small conductance (SK) channel plays a key role in K secretion in the cortical collecting duct (CCD). A high-K intake stimulates renal K secretion and involves a significant increase in the number of SK channels in the apical membrane of the CCD. We used the patch-clamp technique to examine the role of protein tyrosine kinase (PTK) in regulating the activity of SK channels in the CCD. The application of 100 μM genistein stimulated SK channels in 11 of 12 patches in CCDs from rats on a K-deficient diet, and the mean increase in NP o, a product of channel number ( N) and open probabil

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Zhang, H., Y. Huang, and H. L. Chen. "Isozymes of nuclear protein tyrosine kinase during chemical induced hepatocarcinogenesis in rats." Journal of Cancer Research and Clinical Oncology 123, no. 9 (1997): 527–29. http://dx.doi.org/10.1007/s004320050099.

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Zhang, H., Y. Huang, and H. L. Chen. "Isozymes of nuclear protein tyrosine kinase during chemical induced hepatocarcinogenesis in rats." Journal of Cancer Research and Clinical Oncology 123, no. 9 (1997): 527–29. http://dx.doi.org/10.1007/bf01192209.

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Qi, A., T. Wuliji, and Y. Zhang. "395 OVINE SPERMATOGONIA ISOLATION AND IN VITRO GROWTH ASSAY." Reproduction, Fertility and Development 22, no. 1 (2010): 354. http://dx.doi.org/10.1071/rdv22n1ab395.

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Spermatogonia, as adult stem cells from the male reproductive system, are attracting strong interest from those studying male reproductive gamete preservation and developing new approaches in transgenic animals. Protein gene product 9.5 (PGP9.5) is a marker of sheep spermatogonia, which has been validated by J. R. Rodriguez-Sosa et al. (2006).The objective of this study was to develop an in vitro culture system for spermatogonial growth. Twenty 2- to 3-month-old rams were randomly selected at a local slaughter house for testicular tissue collection. Two-step enzymatic digestion methods were us

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Lin, Dao-Hong, Hyacinth Sterling, Kenneth M. Lerea, et al. "K depletion increases protein tyrosine kinase-mediated phosphorylation of ROMK." American Journal of Physiology-Renal Physiology 283, no. 4 (2002): F671—F677. http://dx.doi.org/10.1152/ajprenal.00160.2002.

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We purified His-tagged ROMK1 and carried out in vitro phosphorylation assays with 32P-radiolabeled ATP to determine whether ROMK1 protein is a substrate for PTK. Addition of active c-Src and [32P]ATP to the purified ROMK1 protein resulted in the phosphorylation of the ROMK1 protein. However, c-Src did not phosphorylate R1Y337A in which tyrosine residue 337 was mutated to alanine. Furthermore, phosphopeptide mapping identified two phosphopeptides from the trypsin-digested ROMK1 protein. In contrast, no phosphorylated peptide has been found in the trypsin-digested R1Y337A protein. This suggested

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Gerrard, Gareth, Caroline Cloke, Ian H. Gabriel, Katayoun Rezvani, Jane F. Apperley, and Letizia Foroni. "WT1 and PRAME Expression in CML: Quantitation by Real-Time PCR and Correlation with BCR-ABL Expression Levels." Blood 112, no. 11 (2008): 4225. http://dx.doi.org/10.1182/blood.v112.11.4225.4225.

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Abstract Introduction. Chronic myeloid leukaemia (CML) is a malignant clonal myeloproliferative disorder characterised by the presence of the Philadelphia chromosome (Ph) and expression of the BCR-ABL fusion protein with constitutive tyrosine kinase activity and multi-target dysregulation. Standard therapy employs the use of tyrosine kinase inhibitors, such as Imatinib mesylate, with a high level of success. However, in a proportion of patients this approach is sub-optimal necessitating the use other treatment modalities. Allogeneic stem cell transplantation (allo-SCT) is established and poten

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Demeule, Michel, Sylvie Giroux, Gérard F. Murphy, and Richard Béliveau. "Cyclosporin treatment alters protein phosphorylation in kidney membranes." Biochemistry and Cell Biology 72, no. 3-4 (1994): 143–51. http://dx.doi.org/10.1139/o94-021.

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Phosphorylation, protein carboxyl methylation, and ADP-ribosylation were assayed in renal basolateral membranes and brush border membranes isolated from rats treated by subcutaneous administration of 5 or 10 mg/(kg∙day) of cyclosporin A (CsA) for 10 days to investigate potential alterations in signal transduction in kidney cortex. Protein carboxyl methylation of class II measured in membranes and in cytosolic fraction was not affected by CsA treatment. ADP-ribosylation performed in the presence of pertussis or cholera toxin was also similar in control and treated rats. However, changes in phos

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Craig, Andrew W. B., Ralph Zirngibl, Karen Williams, Lesley-Ann Cole, and Peter A. Greer. "Mice Devoid of Fer Protein-Tyrosine Kinase Activity Are Viable and Fertile but Display Reduced Cortactin Phosphorylation." Molecular and Cellular Biology 21, no. 2 (2001): 603–13. http://dx.doi.org/10.1128/mcb.21.2.603-613.2001.

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ABSTRACT The ubiquitous Fer protein-tyrosine kinase has been proposed to regulate diverse processes such as cell growth, cell adhesion, and neurite outgrowth. To gain insight into the biological function of Fer, we have targeted the fer locus with a kinase-inactivating missense mutation (ferD743R ). Mice homozygous for this mutation develop normally, have no overt phenotypic differences from wild-type mice, and are fertile. Since these mice lack both Fer and the testis-specific FerT kinase activities, these proteins are clearly not essential for development and survival. No differences were ob

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Piccoletti, R., P. Maroni, P. Bendinelli, and A. Bernelli-Zazzera. "Rapid stimulation of mitogen-activated protein kinase of rat liver by prolactin." Biochemical Journal 303, no. 2 (1994): 429–33. http://dx.doi.org/10.1042/bj3030429.

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Intraperitoneal prolactin administration to female rats caused a rapid and transient stimulation of hepatic mitogen-activated kinase (MAP kinase) activity measured in vitro as cytosolic phosphotransferase capacity towards two specific substrates. Myelin basic protein kinase activity of MAP kinase immunoprecipitates confirmed the specificity and magnified the prolactin effect. Immunoblot experiments with anti-(MAP kinase) and anti-phosphotyrosine antibodies showed changes in both electrophoretic mobility and phosphotyrosine content of 40 and 44 kDa isoenzymes suggesting that prolactin affects t

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Hei, Yong-Jiang, Xunsheng Chen, Jack Diamond, and John H. McNeill. "Distribution of MAP kinase, S6 kinase, and casein kinase II in rat tissues: activation by insulin in spleen." Biochemistry and Cell Biology 72, no. 1-2 (1994): 49–53. http://dx.doi.org/10.1139/o94-008.

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We examined the distribution of mitogen-activated protein (MAP) kinase, S6 kinase, and casein kinase II (CK-II) in the muscle, spleen, brain, and testes of Wistar rats. It was observed that spleen extracts contained the highest activity of all the kinases. Anion-exchange chromatography of spleen extracts by a MonoQ column resolved a single peak of myelin basic protein phosphotransferase activity that eluted after the usual position of the previously described p42 and p44 MAP kinases. Immunoblotting of the peak fractions with anti-MAP kinase antibody did not detect any immunoreactive bands that

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Singh, Durgesh Kumar, Rohit Kumar Deshmukh, Praveen Kumar Narayanan, Sisinthy Shivaji, and Archana Bharadwaj Siva. "SRC family kinases in hamster spermatozoa: evidence for the presence of LCK." Reproduction 153, no. 5 (2017): 655–69. http://dx.doi.org/10.1530/rep-16-0591.

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Sperm capacitation is a prerequisite for successful fertilization. Increase in tyrosine phosphorylation is considered the hallmark of capacitation and attempts to understand its regulation are ongoing. In this regard, we attempted to study the role of SRC family kinases (SFKs) in the hamster sperm functions. Interestingly, we found the presence of the lymphocyte-specific protein tyrosine kinase, LCK, in mammalian spermatozoa and further characterized it in terms of its localization and function. LCK was found in spermatozoa of several species, and its transcript was identified in the hamster t

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Maru, Y., H. Hirai, M. C. Yoshida, and F. Takaku. "Evolution, expression, and chromosomal location of a novel receptor tyrosine kinase gene, eph." Molecular and Cellular Biology 8, no. 9 (1988): 3770–76. http://dx.doi.org/10.1128/mcb.8.9.3770.

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Partial sequence analysis of the genomic eph locus revealed that the splicing points of kinase domain-encoding exons were completely distinct from those of the other protein tyrosine kinase members reported, suggesting that this is the earliest evolutionary split within this family. In Northern (RNA) blot analysis, the eph gene was expressed in liver, lung, kidney, and testis of rat, and screening of 25 human cancers of various cell types showed preferential expression in cells of epithelial origin. Overexpression of eph mRNA was found in a hepatoma and a lung cancer without gene amplification

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Maru, Y., H. Hirai, M. C. Yoshida, and F. Takaku. "Evolution, expression, and chromosomal location of a novel receptor tyrosine kinase gene, eph." Molecular and Cellular Biology 8, no. 9 (1988): 3770–76. http://dx.doi.org/10.1128/mcb.8.9.3770-3776.1988.

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Partial sequence analysis of the genomic eph locus revealed that the splicing points of kinase domain-encoding exons were completely distinct from those of the other protein tyrosine kinase members reported, suggesting that this is the earliest evolutionary split within this family. In Northern (RNA) blot analysis, the eph gene was expressed in liver, lung, kidney, and testis of rat, and screening of 25 human cancers of various cell types showed preferential expression in cells of epithelial origin. Overexpression of eph mRNA was found in a hepatoma and a lung cancer without gene amplification

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Zhu, Ding Liang, Thierry Herembert, and Pierre Marche. "Mitogenic Events Induced by Vasopressin in Aortic Fibroblasts from Spontaneously Hypertensive Rats." Clinical Science 85, no. 1 (1993): 57–61. http://dx.doi.org/10.1042/cs0850057.

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1. To further explore the mechanisms of arterial growth, we investigated the signalling pathways through which arginine-vasopressin acts as a mitogen in cultured adventitial aortic fibroblasts of the spontaneously hypertensive rat, and we examined the mechanisms involved in the hyperresponsiveness to arginine-vasopressin of fibroblasts from spontaneously hypertensive rats compared with fibroblasts from Wistar-Kyoto rats. 2. Arginine-vasopressin-induced [3H]thymidine incorporation was used to determine the peptide mitogenicity. Arginine-vasopressin-triggered hydrolysis of phosphoinositides by p

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Lima, Fabio B., Ubiratan F. Machado, Ione Bartol, et al. "Pinealectomy causes glucose intolerance and decreases adipose cell responsiveness to insulin in rats." American Journal of Physiology-Endocrinology and Metabolism 275, no. 6 (1998): E934—E941. http://dx.doi.org/10.1152/ajpendo.1998.275.6.e934.

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Although the pineal gland influences several physiological systems, only a few studies have investigated its role in the intermediary metabolism. In the present study, male Wistar rats, pinealectomized or sham-operated 6 wk before the experiment, were submitted to both intravenous glucose tolerance tests (IVGTT) and insulin binding as well as glucose transport assays in isolated adipocytes. The insulin receptor tyrosine kinase activity was assessed in liver and muscle. The insulin secretory response during the IVGTT was impaired, particularly in the afternoon, and the glucose transport respons

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Cheung, Herman H., Norio Takagi, Lucy Teves, Richard Logan, M. Christopher Wallace, and James W. Gurd. "Altered Association of Protein Tyrosine Kinases with Postsynaptic Densities after Transient Cerebral Ischemia in the Rat Brain." Journal of Cerebral Blood Flow & Metabolism 20, no. 3 (2000): 505–12. http://dx.doi.org/10.1097/00004647-200003000-00009.

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Transient cerebral ischemia results in an increase in the tyrosine phosphorylation of proteins associated with postsynaptic densities (PSDs). The authors investigated the possible mechanisms behind this increase by analyzing isolated PSDs for protein tyrosine kinase activity and for the presence of specific tyrosine kinases. Transient (15 minutes) global ischemia was produced in adult rats by four-vessel occlusion, and PSDs were isolated immediately after ischemia or after 20 minutes or 6 hours of reperfusion. Tyrosine phosphorylation of several PSD proteins, including the N-methyl-d-aspartate

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Cybulsky, A. V., P. R. Goodyer, and A. J. McTavish. "Epidermal growth factor receptor activation in developing rat kidney." American Journal of Physiology-Renal Physiology 267, no. 3 (1994): F428—F436. http://dx.doi.org/10.1152/ajprenal.1994.267.3.f428.

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Epidermal growth factor (EGF) binding increases in late-gestational rat kidney and then falls toward basal adult levels postnatally during the 1st wk. We report that the increase in EGF binding is accompanied by an increase in EGF receptor (EGFR) protein and activation of EGFR tyrosine kinase. Multiple proteins were endogenously tyrosine phosphorylated in kidney membranes from fetal rats, and the phosphorylation pattern was similar in rats ranging from 16 to 21 days of gestation. Tyrosine phosphorylation was, however, almost undetectable in 12-wk adult rat kidneys (controls). Among the phospho

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Tureaud, J., F. H. Sarkar, S. E. Fligiel, et al. "Increased expression of EGFR in gastric mucosa of aged rats." American Journal of Physiology-Gastrointestinal and Liver Physiology 273, no. 2 (1997): G389—G398. http://dx.doi.org/10.1152/ajpgi.1997.273.2.g389.

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Although in Fischer-344 rats aging is found to be associated with increased gastric mucosal proliferative activity, little is known about the intracellular events that regulate this process. The present investigation examines the age-related changes in gastric mucosal tyrosine kinase activity and expression of epidermal growth factor receptor(EGFR) and its structural and functional analog p185c-erbB-2, the protein product of c-erbB-2/c-neu protooncogene. We observed a significantly higher intrinsic tyrosine kinase activity and tyrosine phosphorylation of EGFR and p185c-erbB-2 in the gastric mu

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Nomoto, Yoshitsugu, Yohsuke Miyagawa, Tadayoshi Shiraishi, et al. "Tyrosine-Specific Protein Kinase Participates in the Pathogenesis of Acute Immune Complex Alveolitis in Rats." Respiration 61, no. 1 (1994): 19–22. http://dx.doi.org/10.1159/000196307.

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Lin, Dao-Hong, Hyacinth Sterling, Baofeng Yang, Steven C. Hebert, Gerhard Giebisch, and Wen-Hui Wang. "Protein tyrosine kinase is expressed and regulates ROMK1 location in the cortical collecting duct." American Journal of Physiology-Renal Physiology 286, no. 5 (2004): F881—F892. http://dx.doi.org/10.1152/ajprenal.00301.2003.

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We previously demonstrated that dietary K intake regulates the expression of Src family PTK, which plays an important role in controlling the expression of ROMK1 in plasma membrane (Wei Y, Bloom P, Lin D-H, Gu RM, and Wang WH. Am J Physiol Renal Physiol 281: F206–F212, 2001). In the present study, we used the immunofluorescence staining technique to demonstrate the presence of c-Src, a member of Src family PTK, in the thick ascending limb (TAL) and collecting duct. Confocal microscopy shows that c-Src is highly expressed in the renal cortex and outer medulla. Moreover, c-Src and ROMK are coexp

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47

Rivard, N., G. Rydzewska, J. S. Lods, J. Martinez, and J. Morisset. "Pancreas growth, tyrosine kinase, PtdIns 3-kinase, and PLD involve high-affinity CCK-receptor occupation." American Journal of Physiology-Gastrointestinal and Liver Physiology 266, no. 1 (1994): G62—G70. http://dx.doi.org/10.1152/ajpgi.1994.266.1.g62.

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Cholecystokinin (CCK), CCK octapeptide (CCK-8), and caerulein (Cae) are the most potent trophic factors in the pancreas when given exogenously or released from the intestine. Recent studies have suggested that this growth-promoting effect of CCK was initiated through the occupation of the CCKA receptor. This study was then undertaken to determine whether occupation of the high- or low-affinity CCKA receptor is involved in the growth process and to establish which transduction signals have been selectively activated. As an answer to the first question, rats were infused with CCK JMV-180, a CCKA

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Dosil, M., S. Wang, and I. R. Lemischka. "Mitogenic signalling and substrate specificity of the Flk2/Flt3 receptor tyrosine kinase in fibroblasts and interleukin 3-dependent hematopoietic cells." Molecular and Cellular Biology 13, no. 10 (1993): 6572–85. http://dx.doi.org/10.1128/mcb.13.10.6572.

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Flk2/Flt3 is a recently identified receptor tyrosine kinase expressed in brain, placenta, testis, and primitive hematopoietic cells. The mitogenic signalling potential and biochemical properties of Flk2/Flt3 have been analyzed by using a chimeric receptor composed of the extracellular domain of the human colony-stimulating factor 1 receptor and the transmembrane and cytoplasmic domains of murine Flk2/Flt3. We demonstrate that colony-stimulating factor 1 stimulation of the Flk2/Flt3 kinase in transfected NIH 3T3 fibroblasts leads to a transformed phenotype and generates a full proliferative res

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Dosil, M., S. Wang, and I. R. Lemischka. "Mitogenic signalling and substrate specificity of the Flk2/Flt3 receptor tyrosine kinase in fibroblasts and interleukin 3-dependent hematopoietic cells." Molecular and Cellular Biology 13, no. 10 (1993): 6572–85. http://dx.doi.org/10.1128/mcb.13.10.6572-6585.1993.

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Flk2/Flt3 is a recently identified receptor tyrosine kinase expressed in brain, placenta, testis, and primitive hematopoietic cells. The mitogenic signalling potential and biochemical properties of Flk2/Flt3 have been analyzed by using a chimeric receptor composed of the extracellular domain of the human colony-stimulating factor 1 receptor and the transmembrane and cytoplasmic domains of murine Flk2/Flt3. We demonstrate that colony-stimulating factor 1 stimulation of the Flk2/Flt3 kinase in transfected NIH 3T3 fibroblasts leads to a transformed phenotype and generates a full proliferative res

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50

Carter, Rebecca W., та Nancy L. Kanagy. "Tyrosine kinases regulate intracellular calcium during α2-adrenergic contraction in rat aorta". American Journal of Physiology-Heart and Circulatory Physiology 283, № 4 (2002): H1673—H1680. http://dx.doi.org/10.1152/ajpheart.01034.2001.

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We have demonstrated enhanced contractile sensitivity to the α2-adrenoreceptor (α2-AR) agonist UK-14304 in arteries from rats made hypertensive with chronic nitric oxide synthase (NOS) inhibition (LHR) compared with arteries from normotensive rats (NR); additionally, this contraction requires Ca2+ entry. We hypothesized that tyrosine kinases augment α2-AR contraction in LHR arteries by increasing Ca2+. The tyrosine kinase inhibitor tyrphostin 23 significantly attenuated UK-14304 contraction of denuded thoracic aortic rings from NR and LHR. However, tyrphostin 23 did not alter UK-14304 contract

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