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Journal articles on the topic 'Proteine antigelo'

Author: Grafiati

Published: 9 March 2023

Last updated: 19 July 2025

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1

Fernández-Quintero, Monica L., Johannes R. Loeffler, Franz Waibl, Anna S. Kamenik, Florian Hofer, and Klaus R. Liedl. "Conformational selection of allergen-antibody complexes—surface plasticity of paratopes and epitopes." Protein Engineering, Design and Selection 32, no. 11 (2019): 513–23. http://dx.doi.org/10.1093/protein/gzaa014.

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Abstract Antibodies have the ability to bind various types of antigens and to recognize different antibody-binding sites (epitopes) of the same antigen with different binding affinities. Due to the conserved structural framework of antibodies, their specificity to antigens is mainly determined by their antigen-binding site (paratope). Therefore, characterization of epitopes in combination with describing the involved conformational changes of the paratope upon binding is crucial in understanding and predicting antibody-antigen binding. Using molecular dynamics simulations complemented with str

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O’Rourke, Sara M., Giora I. Morozov, Jacob T. Roberts, Adam W. Barb, and Nikolaos G. Sgourakis. "Production of soluble pMHC-I molecules in mammalian cells using the molecular chaperone TAPBPR." Protein Engineering, Design and Selection 32, no. 12 (2019): 525–32. http://dx.doi.org/10.1093/protein/gzaa015.

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Abstract Current approaches for generating major histocompatibility complex (MHC) Class-I proteins with desired bound peptides (pMHC-I) for research, diagnostic and therapeutic applications are limited by the inherent instability of empty MHC-I molecules. Using the properties of the chaperone TAP-binding protein related (TAPBPR), we have developed a robust method to produce soluble, peptide-receptive MHC-I molecules in Chinese Hamster Ovary cells at high yield, completely bypassing the requirement for laborious refolding from inclusion bodies expressed in E.coli. Purified MHC-I/TAPBPR complexe

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Sinitsyn, B. F. "Detecting a psoriatic antigen analogous to infectious prion proteins." Russian Journal of Infection and Immunity 9, no. 3-4 (2019): 589–94. http://dx.doi.org/10.15789/2220-7619-2019-3-4-589-594.

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Until now, psoriatic antigen as a specific antigen derived from some infectious agent potentially related to origin of psoriasis has not been identified, thereby strongly arguing against infectious theory of psoriasis. However, the lack of specific psoriasis-associated antigens may be theoretically accounted for by an idea that psoriatic antigen could be analogous to infectious prion proteins (PrPSc analogue). It might be identical to some epidermal protein in psoriasis-free subjects that might differ antigenically from related normal protein by resistance to digestive enzymes similarly to PrP

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Adams, John H., and Ronald D. Smith. "Differential extraction of antigens of Anaplasma marginale." American Journal of Veterinary Research 49, no. 2 (1988): 257–60. https://doi.org/10.2460/ajvr.1988.49.02.257.

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SUMMARY Strain-, group-, and genus-specific antigens of the Florida isolate of Anaplasma marginale had different solubilities in zwitterionic, nonionic, and anionic detergents. On the basis of their solubility in nonionic detergent, antigens were grouped into 3 classes: (i) a 108-kilodalton (kD) group-specific antigen and a series of poorly defined antigens (70 to 100 kD) were completely soluble in nonionic detergent; (ii) 96 kD and 75 kD group-specific antigens, and a 91-kD strain-specific antigen were completely insoluble in nonionic detergent; and (iii) a 47-kD group-specific antigen and a

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Bowtell, D. D. L., R. B. Saint, M. D. Rickard, and G. F. Mitchell. "Immunochemical analysis of Taenia taeniaeformis antigens expressed in Escherichia coli." Parasitology 93, no. 3 (1986): 599–610. http://dx.doi.org/10.1017/s0031182000081300.

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SUMMARYPreviously we reported the isolation of several Escherichia coli clones expressing fragments of Taenia taeniaeformis antigens as β-galactosidase fused proteins (Bowtell, Saint, Rickard & Mitchell, 1984). Here we describe the isolation of additional antigen-expressing clones from a larval cDNA library and the assignment of these clones to 7 antigen families. These were isolated with a polyspecific rabbit antiserum raised to the oncosphere. Since this serum was capable of reacting with a large number of antigens, it was important to develop techniques for rapidly determining the ident

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Lajoie, Jason M., Yong Ku Cho, Dustin Frost, Samantha Bremner, Lingjun Li, and Eric V. Shusta. "A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes." Protein Engineering, Design and Selection 32, no. 5 (2019): 219–30. http://dx.doi.org/10.1093/protein/gzz035.

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Abstract Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening of antibodies that bind to membrane protein complexes, enabling discovery of antibodies that target antigens involved in a functional protein-protein interaction of interest. For this proof-of-concept study, we focused on the receptor-mediated endocytosis machinery at the blood-brain barri

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Delaney, Kristen N., and Steven B. Mizel. "A vaccine containing recombinant poxvirus proteins and flagellin promotes protective immunity against vaccinia virus (132.17)." Journal of Immunology 182, no. 1_Supplement (2009): 132.17. http://dx.doi.org/10.4049/jimmunol.182.supp.132.17.

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Abstract Bacterial flagellin is a potent adjuvant that enhances adaptive immune responses to a variety of antigens. The vaccinia virus antigens L1R and B5R are highly immunogenic in the context of the parent virus, but recombinant forms of the proteins are only weakly immunogenic. Therefore, we evaluated the response to these antigens when flagellin was used as an adjuvant. We found that flagellin promoted a robust antigen-specific humoral response to poxvirus antigens delivered intranasally and intramuscularly, but intramuscular immunization was more efficient. Flagellin/poxvirus antigen fusi

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Hassan, Husain, Tariq S. AL-Hadithi, and Hadi M. Al-Sakee. "Experimental trial with a heat-shocked protoscolex extract as a vaccine candidate for protection against hydatid disease." Turkiye Parazitol Derg 40 (February 6, 2016): 1–8. https://doi.org/10.5152/tpd.2016.3993.

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Cystic echinococcosis is distributed worldwide and is an important public health challenge in many countries. The present study was an experimental trial to use hydatid antigens derived from viable protoscoleces cultivated at 37 and 45°C for 4 h as a vaccine candidate for protection against hydatid infection. Methods: Balb/c mice were immunized with hydatid antigens extracted from protoscoleces exposed to 37 and 45°C as well as partially purified hydatid antigens containing 30, 60, and 90 µg of heat shock protein 70 administered with or without an adjuvant. Results: Crude antigen

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van der Kant, Rob, Joschka Bauer, Anne R. Karow-Zwick та ін. "Adaption of human antibody λ and κ light chain architectures to CDR repertoires". Protein Engineering, Design and Selection 32, № 3 (2019): 109–27. http://dx.doi.org/10.1093/protein/gzz012.

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Abstract Monoclonal antibodies bind with high specificity to a wide range of diverse antigens, primarily mediated by their hypervariable complementarity determining regions (CDRs). The defined antigen binding loops are supported by the structurally conserved β-sandwich framework of the light chain (LC) and heavy chain (HC) variable regions. The LC genes are encoded by two separate loci, subdividing the entity of antibodies into kappa (LCκ) and lambda (LCλ) isotypes that exhibit distinct sequence and conformational preferences. In this work, a diverse set of techniques were employed including m

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Satyaprakash, Kaushik, Wiqar Ahmed Khan, Nandkishor Namdeorao Zade, Sandeep Prabhakarrao Chaudhari, and Shilpshri Vasant Shinde. "Characterization of somatic and metabolic antigens of Cysticercus cellulosae and determination of immunodominant proteins." Veterinarski arhiv 92, no. 4 (2022): 399–409. http://dx.doi.org/10.24099/vet.arhiv.1300.

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The present study shows the characterization of four somatic antigens (Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA) and Membrane-Body Antigen (MBA)) and one metabolic antigen (Excretory-Secretory Antigen (ESA)) prepared from Cysticercus cellulosae originated from a naturally infected pig. Immunodominat proteins were determined using hyper immune rabbit sera. The Sodium Dodecyl SulphatePolyacrylamide Gel Electrophoresis (SDS-PAGE) profile of the antigens revealed different numbers and patterns of protein bands in the range of 11.80 to 176.74 kDa. The electrophoretic

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Rennert, Paul, Lan Wu, Lihe Su, Roy Lobb, and Christine Ambrose. "160 Evaluation and development of dual and triple antigen targeting CAR-T Engager proteins for Her2-positive CNS metastases and solid tumors." Journal for ImmunoTherapy of Cancer 9, Suppl 2 (2021): A170. http://dx.doi.org/10.1136/jitc-2021-sitc2021.160.

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BackgroundSolid tumors display pronounced antigen heterogeneity and clinical studies have shown that antigen escape from therapy occurs rapidly, limiting the persistence and efficacy of CAR T cells. Here we present dual and triple-antigen binding proteins that bridge CAR T cells to multiple antigens, allowing a simultaneous attack on tumor antigens by a single CAR T antibody domain. We call these CAR T Engager proteins. CAR T Engager proteins can be encoded into lentiviral vectors for secretion from CAR T cells, can be encoded into oncolytic viral vectors for secretion from transduced tumor ce

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Di Benedetto, Roberta, Luisa Massai, Mark Wright, et al. "Adjuvanted Modified Bacterial Antigens for Single-Dose Vaccines." International Journal of Molecular Sciences 25, no. 21 (2024): 11461. http://dx.doi.org/10.3390/ijms252111461.

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Alum is the most used vaccine adjuvant, due to its safety, low cost and adjuvanticity to various antigens. However, the mechanism of action of alum is complex and not yet fully understood, and the immune responses elicited can be weak and antigen-dependent. While several antigens rapidly desorb from alum upon exposure to serum, phosphorylated proteins remain tightly bound through a ligand-exchange reaction with surface hydroxyls on alum. Here, bacterial proteins and glycoconjugates have been modified with phosphoserines, aiming at enhancing the binding to alum and prolonging their bioavailabil

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Folkvord, J. M., D. Viders, A. Coleman-Smith, and R. A. Clark. "Optimization of immunohistochemical techniques to detect extracellular matrix proteins in fixed skin specimens." Journal of Histochemistry & Cytochemistry 37, no. 1 (1989): 105–13. http://dx.doi.org/10.1177/37.1.2461979.

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Complete antigen visualization in the context of well-preserved tissue architecture is the goal of all immunohistochemical techniques. Frozen tissue section techniques achieve optimal antigen visualization but preserve tissue architecture poorly. On the other hand, formalin-fixed tissue section techniques preserve tissue architecture very well but result in antigen masking. Enzymatic digestion or salt extraction of formalin-fixed sections has been used to reestablish antigen expression. Recently acid-alcohol-fixed tissue has been used as a successful compromise between tissue architecture pres

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Hicham, Bouabe PhD. "Polypeptide Rearrangement Hypothesis and It's Implication in Genetic Diversity." Journal of Proteomics & Bioinformatics 1, no. 7 (2008): 336–45. https://doi.org/10.4172/jpb.1000042.

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Protein splicing is a post-translational process, in which a nested intervening sequence (intein) is spliced out of the interior of a polypeptide precursor, and the flanking protein fragments (exteins) are ligated to form a mature protein. This process was identified in yeast, bacteria and the plant jackbean, and recently for <strong>MHC class I</strong> antigen processing in vertebrates. Thus, it seems very likely that, besides antigens, functional proteins could be synthesized by post-translational splicing in vertebrates. Protein splicing indicates that proteins, after their trans

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Elkon, K. B., A. P. Parnassa, and C. L. Foster. "Lupus autoantibodies target ribosomal P proteins." Journal of Experimental Medicine 162, no. 2 (1985): 459–71. http://dx.doi.org/10.1084/jem.162.2.459.

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All nine SLE (systemic lupus erythematosus) sera with antiribosomal antibody activity targeted the same three ribosomal protein antigens, of molecular masses 38 and 17/19 kD when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. One serum reacted with an additional protein of approximately kD. Ribosomal subunit fractionation by composite gel electrophoresis and sucrose density ultracentrifugation showed that these proteins were part of the large subunit. Isoelectric focusing in agarose, and two-dimensional polyacrylamide gel electrophoresis revealed th

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Borovikov, S. N., А. Syzdykova, Z. A. Museipova, and T. G. Bakishev. "STUDYING THE DIAGNOSTIC VALUE OF RECOMBINANT CAMPYLO-BACTER JEJUNI ANTIGENS." HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY : Veterinary sciences, no. 2(002) (June 13, 2023): 20–26. http://dx.doi.org/10.51452/kazatuvc.2023.2(002).1408.

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This article describes the results of a study on the diagnostic value of Campylobacter jejuni recombinant antigens, Campylobacter Omp18 protein and major outer membrane protein (MOMP). In the present study, these proteins were used as antigens in enzyme im-munoassays to detect specific antibodies in the serum of cattle. Commercial native protein antigens were used to compare the effectiveness with similar studies. In total, 95 blood se rum samples from cattle from various farms in the northern region of the Republic of Ka-zakhstan were used. The greatest number of positive results was observed

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Bech-Nielsen, Steen, Linda L. Burianek, Elisabeth Spangler, Lawrence E. Heider, Glen F. Hoffsis, and C. Richard Dorn. "Characterization of Mycobacterium paratuberculosis antigenic proteins." American Journal of Veterinary Research 46, no. 11 (1985): 2418–20. https://doi.org/10.2460/ajvr.1985.46.11.2418.

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SUMMARY The characterization of a purified antigen from Mycobacterium paratuberculosis, recently made commercially available for use in serodiagnosis by enzyme-linked immunosorbent assay (elisa), of paratuberculosis in cattle was described. This assay had 89% specificity and 83% sensitivity for M paratuberculosis infection. The protein/polypeptide composition of the purified antigen was compared with that of a crude protoplasmic extract of strain 18 M paratuberculosis used in the agar-gel immunodiffusion test and elisa and with that of sonicated strain 19698 M paratuberculosis organisms grown

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Sette, A., L. Adorini, S. M. Colon, S. Buus, and H. M. Grey. "Capacity of intact proteins to bind to MHC class II molecules." Journal of Immunology 143, no. 4 (1989): 1265–67. http://dx.doi.org/10.4049/jimmunol.143.4.1265.

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Abstract Here we have demonstrated that denatured, but not native protein antigens can interact with Ia molecules. Thus, the failure of native antigens to be recognized as such by T cells appears to be at least in part due to a deficient antigen/Ia interaction. These results also support previous observations that some T cells can recognize denatured antigens without a further processing requirement. Moreover, a striking correlation was observed between the in vitro binding pattern of denatured proteins and the pattern of restriction of T cell responses elicited by immunization with the native

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Milosevic, Slavoljub, Uta Behrends, Dinesh Adhikary, and Josef Mautner. "Identification of Major Histocompatibility Complex Class II-Restricted Antigens and Epitopes of the Epstein-Barr Virus by a Novel Bacterial Expression Cloning Approach." Journal of Virology 80, no. 21 (2006): 10357–64. http://dx.doi.org/10.1128/jvi.01193-06.

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ABSTRACT Epstein-Barr virus (EBV)-specific T cells have been successfully used to treat or prevent EBV-positive lymphoproliferative disease in hematopoietic stem cell transplant recipients, but the antigens recognized by the infused CD4+ T cells have remained unknown. Here, we describe a simple procedure that permits the identification of viral T-helper (TH)-cell antigens and epitopes. This direct antigen identification method is based on the random expression of viral polypeptides fused to chloramphenicol acetyltransferase (CAT) in bacteria, which are subsequently fed to major histocompatibil

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Hisham, Yasmin, and Yaqoub Ashhab. "Identification of Cross-Protective Potential Antigens against PathogenicBrucellaspp. through Combining Pan-Genome Analysis with Reverse Vaccinology." Journal of Immunology Research 2018 (December 9, 2018): 1–15. http://dx.doi.org/10.1155/2018/1474517.

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Brucellosis is a zoonotic infectious disease caused by bacteria of the genusBrucella.Brucella melitensis,Brucella abortus, andBrucella suisare the most pathogenic species of this genus causing the majority of human and domestic animal brucellosis. There is a need to develop a safe and potent subunit vaccine to overcome the serious drawbacks of the live attenuatedBrucellavaccines. The aim of this work was to discover antigen candidates conserved among the three pathogenic species. In this study, we employed a reverse vaccinology strategy to compute the core proteome of 90 completed genomes: 55B

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Celedon, Paola Alejandra Fiorani, Leonardo Maia Leony, Ueriton Dias Oliveira, et al. "Stability Assessment of Four Chimeric Proteins for Human Chagas Disease Immunodiagnosis." Biosensors 11, no. 8 (2021): 289. http://dx.doi.org/10.3390/bios11080289.

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The performance of an immunoassay relies on antigen-antibody interaction; hence, antigen chemical stability and structural integrity are paramount for an efficient assay. We conducted a functional, thermostability and long-term stability analysis of different chimeric antigens (IBMP), in order to assess effects of adverse conditions on four antigens employed in ELISA to diagnose Chagas disease. ELISA-based immunoassays have served as a model for biosensors development, as both assess molecular interactions. To evaluate thermostability, samples were heated and cooled to verify heat-induced dena

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Izhberdeeva, Margarita P., Anastasiya A. Sautkina, Irina A. Barkova, and Dmitry V. Viktorov. "Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants." Journal of microbiology, epidemiology and immunobiology 100, no. 2 (2023): 203–8. http://dx.doi.org/10.36233/0372-9311-284.

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Introduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein. Purpose. To determine the antigenic identity of immunodominant proteins of different genovariants of B. anthracis isolated by gel chromatography and electrophoresis. Materials and methods. Culture filtrates of isogenic variants of B. anthracis strain 575/122 (pXO1+, pXO2+): R01 (pXO1+, pXO2); R00 (pXO1, pXO2) were used in the study. Gel chromatographic fractionation and ele

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Shin, A.-Rum, Kil-Soo Lee, Kang In Lee, et al. "Serodiagnostic Potential of Mycobacterium avium MAV2054 and MAV5183 Proteins." Clinical and Vaccine Immunology 20, no. 2 (2012): 295–301. http://dx.doi.org/10.1128/cvi.00649-12.

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ABSTRACTTheMycobacterium avium-M. intracellularecomplex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnosticM. tuberculosisantigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Amon

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Shortreed, Nicholas A., Anjali J. Panicker, Ailsa J. Dalgliesh, Katherine V. Gates, and Leigh G. Griffiths. "Immunoproteomic identification of in vivo response toward residual antigenic proteins in xenogeneic heart valve biomaterials." Journal of Immunology 204, no. 1_Supplement (2020): 161.9. http://dx.doi.org/10.4049/jimmunol.204.supp.161.9.

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Abstract Valve replacement is an important therapeutic approach for management of valvular heart disease, accounting for over 200,000 surgeries annually worldwide. Deficiencies of current heart valve prostheses led the NHLBI cardiac surgery working group to highlight the need for improved heart valve leaflet biomaterials. Recently, our group has engineered a novel method to eliminate antigens from bovine pericardium (BP) extracellular matrix (ECM) scaffolds, while retaining biomaterial structure-function properties and regenerative capacity. However, despite reducing BP-ECM scaffold antigen bu

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Otsuyama, Ken-ichiro, Hidehiro Tsuneoka, Kaori Kondou, et al. "Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using RefinedN-Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease." Journal of Clinical Microbiology 54, no. 4 (2016): 1058–64. http://dx.doi.org/10.1128/jcm.03009-15.

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The conventional anti-Bartonella henselaeIgM enzyme-linked immunosorbent assay (IgM-ELISA) methods for diagnosing cat scratch disease (CSD) remain poor in both sensitivity and specificity. We sought to develop an IgM-ELISA with improved accuracy in the serodiagnosis of CSD by exploring the antigens that are most suitable for an ELISA. We prepared 5 different protein antigens: antigen I (sonicatedB. henselaewhole-cell antigen), antigen II (N-lauroyl-sarcosine-insoluble antigen), antigen III (processed sarcosine-soluble antigen), and antigen IV and antigen V (sarcosine-insoluble and sarcosine-so

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Valdez, Yanet, Weiling Mah, Monte M. Winslow, Lixin Xu, Peter Ling та Sarah E. Townsend. "Major Histocompatibility Complex Class II Presentation of Cell-associated Antigen Is Mediated by CD8α+ Dendritic Cells In Vivo". Journal of Experimental Medicine 195, № 6 (2002): 683–94. http://dx.doi.org/10.1084/jem.20010898.

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Antigen-specific B cells express major histocompatibility complex class II and can present antigen directly to T cells. Adoptive transfer experiments using transgenic B and T cells demonstrated that antigen-specific B cells can also efficiently transfer antigen to another cell for presentation to T cells in vivo. To identify the antigen-presenting cell that receives antigens from B cells, a strategy was developed to follow the traffic of B cell–derived proteins in vivo. B cells were labeled with the fluorescent dye CFSE and loaded with antigen, before adoptive transfer into recipient mice. Pop

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Trivedi, Vrunda, Changlin Yang, Elizabeth Ogando-Rivas, Kyle Dyson, and Duane Mitchell. "IMMU-16. IDENTIFICATION OF TUMOR REJECTION ANTIGENS FOR PRECISION ADOPTIVE CELLULAR THERAPY FOR PEDIATRIC GLIOMAS." Neuro-Oncology 25, Supplement_1 (2023): i53. http://dx.doi.org/10.1093/neuonc/noad073.203.

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Abstract BACKGROUND Identifying tumor rejection antigens remains a major barrier to developing effective antigen-directed immunotherapeutics and their application to pediatric brain tumors. Recent progress in the field of cancer immunogenomics has facilitated the search for tumor-specific antigens by applying comprehensive cancer genomics to tumor antigen discovery. Using a custom antigen prediction pipeline, we performed in silico antigen prediction across a broad array of antigen classes including neoantigens, tumor-associated antigens (TAAs), and fusion proteins for human and murine medullo

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Yu, Hong, Karuna P. Karunakaran, Xiaozhou Jiang, Caixia Shen, Peter Andersen, and Robert C. Brunham. "Chlamydia muridarum T Cell Antigens and Adjuvants That Induce Protective Immunity in Mice." Infection and Immunity 80, no. 4 (2012): 1510–18. http://dx.doi.org/10.1128/iai.06338-11.

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ABSTRACTMajor impediments to aChlamydiavaccine lie in discovering T cell antigens and polarizing adjuvants that stimulate protective immunity. We previously reported the discovery of three T cell antigens (PmpG, PmpF, and RplF) via immunoproteomics that elicited protective immunity in the murine genital tract infection model againstChlamydiainfection after adoptive transfer of antigen-pulsed dendritic cells. To expand the T cell antigen repertoire necessary for aChlamydiavaccine, we evaluated 10 newChlamydiaT cell antigens discovered via immunoproteomics in addition to the 3 antigens reported

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Barlow, Avlin K., Xin He, and Charles Janeway. "Exogenously Provided Peptides of a Self-antigen Can Be Processed into Forms that Are Recognized by Self–T Cells." Journal of Experimental Medicine 187, no. 9 (1998): 1403–15. http://dx.doi.org/10.1084/jem.187.9.1403.

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Major histocompatibility complex (MHC) class II molecules can present peptides derived from two different sources. The predominant source of peptide in uninfected antigen presenting cells (APCs) is from self-proteins that are synthesized within the cell and traffic through the MHC class II compartment. The other source of antigen is endocytosed proteins, which includes both self- and foreign proteins. Foreign protein antigens generate adaptive immune responses, whereas self-peptides stabilize the MHC class II heterodimer on the cell surface, allowing positive and negative selection of thymocyt

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Porrás, Analía, Stéphanie Gaillard, and Kathleen Rundell. "The Simian Virus 40 Small-t and Large-T Antigens Jointly Regulate Cell Cycle Reentry in Human Fibroblasts." Journal of Virology 73, no. 4 (1999): 3102–7. http://dx.doi.org/10.1128/jvi.73.4.3102-3107.1999.

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ABSTRACT Focus formation in human diploid fibroblasts (HDF cells) is known to require both the simian virus 40 (SV40) large-T and small-t antigens. Similarly, both SV40 proteins were required to stimulate confluent, density-arrested HDF cells to reenter the cell cycle. This study used defective recombinant adenoviruses to examine the roles of the individual SV40 proteins in altering specific steps in the cell cycle. Small-t antigen and, to a lesser extent, large-T antigen increased the level of the S phase cyclin cyclin A but without increasing the activity of associated cyclin kinases unless

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Brazdova, Andrea, Martina Vermachova, Jarmila Zidkova, Zdenka Ulcova-Gallova, and Gabriel Peltre. "Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility." Open Life Sciences 8, no. 9 (2013): 813–18. http://dx.doi.org/10.2478/s11535-013-0214-9.

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AbstractInfertility affects approximately 10% of couples at reproductive age. Semen constituents may be potential immunogenic structures for women. The aim of our work is to detect and identify sperm proteins interacting with serum IgG antibodies from women with fertility disorders. The biochemical characterization of sperm antigens was performed using one and two dimensional gel electrophoresis, both of which were followed by immunoblotting. The IgG-binding proteins of interest were identified using mass spectrometry. From the serum pool of 30 infertile women, we detected sperm antigens withi

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Krasilnikov, I. V., T. I. Vinogradova, M. Djonovic, et al. "TB skin test recombinant proteins as vaccine candidates." Genes & Cells 17, no. 2 (2022): 47–55. http://dx.doi.org/10.23868/202209007.

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Incomplete protection of BCG vaccines, high variability of tuberculosis strains, together with the growing antibiotic resistance of mycobacterium tuberculosis, actualize the need to develop new anti-tuberculosis vaccines. Several novel experimental candidate vaccines based on recombinant proteins, such as those based on the M. tuberculosis ESAT-6 and CFP-10 antigens, are currently being studied in clinical trials. The genome region coding for ESAT-6 and CFP-10 antigens is deleted in BCG strains, so the BCG-immunized individuals cannot develop an immune response against the recombinant ESAT-6/C

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Venditti, Paola, Paolo Bergamo, Riccardo Talevi, Giovanni sansone, and Paolo Abrescia. "Localisation and capacitation-dependent loss of buffalo sperm-coating antigens shared with rat sperm." Zygote 2, no. 1 (1994): 5–13. http://dx.doi.org/10.1017/s0967199400001714.

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SummaryThe heterodimeric sperm-coating protein CFS was previously localised on the middle-piece region of rat spermatozoa by anti-CFS rabbit antibodies. CFS-immunorelated antigens were detected in the secretion of the water buffalo seminal vesicle by protein electrophoresis and Western blotting. Spermatozoa from buffalo epididymal cauda were incubated with the rat antigen and, upon immunostaining with anti-CFS antibodies and goat anti-rabbit fluorescein isothiocyanate (FITC)-conjugated IgGs, CFS was found attached on both the post-acrosomal region and the tail. Indirect immunofluorescence anal

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Dykman, Lev A., Sergey A. Staroverov, Sergey V. Kozlov, et al. "Immunization of Mice with Gold Nanoparticles Conjugated to Thermostable Cancer Antigens Prevents the Development of Xenografted Tumors." International Journal of Molecular Sciences 23, no. 22 (2022): 14313. http://dx.doi.org/10.3390/ijms232214313.

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Gold nanoparticles as part of vaccines greatly increase antigen stability, antigen accumulation in the lymph nodes, and antigen uptake by antigen-presenting cells. The use of such particles as part of anticancer vaccines based on heat shock proteins to increase vaccine effectiveness is timely. We prepared and characterized nanoconjugates based on 15-nm gold nanoparticles and thermostable tumor antigens isolated from MH22a murine hepatoma cells. The whole-cell lysate of MH22a cells contained the main heat shock proteins. BALB/c mice were injected with the conjugates and then received transplant

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Xu, Xin, Mohamed I. Husseiny, Andreas Goldwich, and Michael Hensel. "Efficacy of Intracellular Activated Promoters for Generation of Salmonella-Based Vaccines." Infection and Immunity 78, no. 11 (2010): 4828–38. http://dx.doi.org/10.1128/iai.00298-10.

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ABSTRACT Salmonella enterica is a versatile vaccine carrier for heterologous antigens. One strategy for vaccine antigen delivery is the use of live attenuated S. enterica strains that translocate heterologous antigens into antigen-presenting cells by means of type III secretion systems (T3SS). The feasibility of this approach has been demonstrated in various experimental vaccination studies. The efficacy of recombinant live vaccines is critically influenced by the optimal level of attenuation and many other factors. For the rational design of approaches involving translocation by T3SS, additio

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Hubbard, A. L., J. R. Bartles, and L. T. Braiterman. "Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies." Journal of Cell Biology 100, no. 4 (1985): 1115–25. http://dx.doi.org/10.1083/jcb.100.4.1115.

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We have localized and identified five rat hepatocyte plasma membrane proteins using hybridoma technology in combination with morphological and biochemical methods. Three different membrane preparations were used as immunogens: isolated hepatocytes, a preparation of plasma membrane sheets that contained all three recognizable surface domains of the intact hepatocyte (sinusoidal, lateral, and bile canalicular), and a glycoprotein subfraction of that plasma membrane preparation. We selected monoclonal IgGs that were hepatocyte specific and localized them using both immunofluorescence on 0.5-micro

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Moens, Ugo, and Andrew Macdonald. "Effect of the Large and Small T-Antigens of Human Polyomaviruses on Signaling Pathways." International Journal of Molecular Sciences 20, no. 16 (2019): 3914. http://dx.doi.org/10.3390/ijms20163914.

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Viruses are intracellular parasites that require a permissive host cell to express the viral genome and to produce new progeny virus particles. However, not all viral infections are productive and some viruses can induce carcinogenesis. Irrespective of the type of infection (productive or neoplastic), viruses hijack the host cell machinery to permit optimal viral replication or to transform the infected cell into a tumor cell. One mechanism viruses employ to reprogram the host cell is through interference with signaling pathways. Polyomaviruses are naked, double-stranded DNA viruses whose geno

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Andrew Nakibinge Kiboneka and Ronnie Mwesigwa. "A primer on immune responses and mechanisms." World Journal of Advanced Research and Reviews 18, no. 2 (2023): 233–43. http://dx.doi.org/10.30574/wjarr.2023.18.2.0814.

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The immune response is how your body recognizes and defends itself against bacteria, viruses, and substances that appear foreign and harmful. Innate, or nonspecific, immunity is the defense system with which you were born. It protects you against all antigens. Innate immunity involves barriers that keep harmful materials from entering your body. An antigen-presenting cell (APC) is a cell that displays antigen bound by major histocompatibility complex (MHC) proteins on its surface; this process is known as antigen presentation. T cells may recognize these complexes using their T cell receptors

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Andrew, Nakibinge Kiboneka, and Mwesigwa Ronnie. "A primer on immune responses and mechanisms." World Journal of Advanced Research and Reviews 18, no. 2 (2023): 233–43. https://doi.org/10.5281/zenodo.8379749.

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The immune response is how your body recognizes and defends itself against bacteria, viruses, and substances that appear foreign and harmful. Innate, or nonspecific, immunity is the defense system with which you were born. It protects you against all antigens. Innate immunity involves barriers that keep harmful materials from entering your body. An antigen-presenting cell (APC) is a cell that displays antigen bound by major histocompatibility complex (MHC) proteins on its surface; this process is known as antigen presentation. T cells may recognize these complexes using their T cell receptors

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Bowden, G. H., N. Nolette, A. S. McKee, and I. R. Hamilton. "The stability of outer-membrane protein and antigen profiles of a strain of Bacteroides intermedius grown in continuous culture at different pH and growth rates." Canadian Journal of Microbiology 37, no. 5 (1991): 368–76. http://dx.doi.org/10.1139/m91-060.

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The stability of the outer-membrane proteins and antigens of a strain of Bacteroides intermedius (VP1 8944 group genotype II) grown in contious culture at varying pH and growth rates (D = 0.025–0.2 h−1, pH 6.0–7.3) has been measured. The membranes showed nine major proteins (> 67–19.55 kilodaltons) and six major antigens (65–28 kilodaltons). Membrane proteins and antigens were stable under the conditions tested; the major proteins were detected in all membranes, and the antigen profiles tested with different antisera showed maximum similarities of 82–95%. Differences did occur in the amount

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Tajuelo, Ana, Eva Gato, Jesús Oteo-Iglesias, et al. "Deep Intraclonal Analysis for the Development of Vaccines against Drug-Resistant Klebsiella pneumoniae Lineages." International Journal of Molecular Sciences 25, no. 18 (2024): 9837. http://dx.doi.org/10.3390/ijms25189837.

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Despite its medical relevance, there is no commercial vaccine that protects the population at risk from multidrug-resistant (MDR) Klebsiella pneumoniae infections. The availability of massive omic data and novel algorithms may improve antigen selection to develop effective prophylactic strategies. Up to 133 exposed proteins in the core proteomes, between 516 and 8666 genome samples, of the six most relevant MDR clonal groups (CGs) carried conserved B-cell epitopes, suggesting minimized future evasion if utilized for vaccination. Antigens showed a range of epitopicity, functional constraints, a

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Doe, W., and P. Pavli. "Antigen Presentation in the Gut." Canadian Journal of Gastroenterology 4, no. 7 (1990): 267–70. http://dx.doi.org/10.1155/1990/527602.

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The induction of T cell responses requires recognition of antigens in association with class II major histocompatibility complex (MHC) proteins and specialized antigen-presenting cells. Candidate antigen-presenting cells in the gut include dendritic cells, macrophages, B lymphocytes, mucosal epithelial cells and endothelial cells. Dendritic cells isolated from normal human colon are potent inducers of primary immune responses and express high levels of class lI MHC proteins. Lamina propria macrophages display class II MHC proteins, can present antigens to sensitized T cells, may process antige

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Mavenyengwa, Rooyen T., Johan A. Maeland, and Sylvester R. Moyo. "Putative Novel Surface-Exposed Streptococcus agalactiae Protein Frequently Expressed by the Group B Streptococcus from Zimbabwe." Clinical and Vaccine Immunology 16, no. 9 (2009): 1302–8. http://dx.doi.org/10.1128/cvi.00133-09.

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ABSTRACTGroup B streptococci (GBS) express a variety of surface-exposed and strain-variable proteins which function as phenotypic markers and as antigens which are able to induce protective immunity in experimental settings. Among these proteins, the chimeric and immunologically cross-reacting alpha-like proteins are particularly important. Another protein, R3, which has been less well studied, occurred at a frequency of 21.5% in GBS from Zimbabwe and, notably, occurred in serotype V strains at a frequency of 75.9%. Working with rabbit antiserum raised against the R3 reference strain ATCC 4944

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Romao, Susana, Nathalie Gasser, Andrea C. Becker, et al. "Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing." Journal of Cell Biology 203, no. 5 (2013): 757–66. http://dx.doi.org/10.1083/jcb.201308173.

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Antigen preservation for presentation is a hallmark of potent antigen-presenting cells. In this paper, we report that in human macrophages and dendritic cells, a subset of phagosomes gets coated with Atg8/LC3, a component of the molecular machinery of macroautophagy, and maintains phagocytosed antigens for prolonged presentation on major histocompatibility complex class II molecules. These Atg8/LC3-positive phagosomes are formed around the antigen with TLR2 agonists and require reactive oxygen species production by NOX2 for their generation. A deficiency in the NOX2-dependent formation of thes

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Geadkaew-Krenc, Amornrat, Rudi Grams, Wansika Phadungsil, et al. "Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis viverrini for Diagnostic Approach." Korean Journal of Parasitology 58, no. 4 (2020): 475–79. http://dx.doi.org/10.3347/kjp.2020.58.4.475.

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Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides

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Leguizamon, M. S., O. E. Campetella, M. B. Reyes, et al. "BloodstreamTrypanosoma cruziparasites from mice simultaneously express antigens that are markers of acute and chronic human Chagas disease." Parasitology 102, no. 3 (1991): 379–85. http://dx.doi.org/10.1017/s0031182000064337.

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Several recombinantTrypanosoma cruziproteins previously isolated were used as antigens to analyse antibody specificities present in sera from human infections. Some parasite proteins such as SAPA (Shed Acute Phase Antigen) are antigenic early after infection. Others, like antigens 1 and 30, are antigenic mainly during the chronic phase of the infection. To understand why different proteins are antigenic at different periods of infection, specificities of antibodies present in the sera of infected mice were compared with the antigens expressed by parasites collected directly from blood. Parasit

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Brázdová, Andrea, M. Vermachová, J. Zídková, et al. "Immunodominant Semen Proteins II: Contribution of Seminal Proteins to Female Immune Infertility." Folia Biologica 59, no. 5 (2013): 198–203. http://dx.doi.org/10.14712/fb2013059050198.

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Seminal fluid is a protective medium for sperm, but it also represents potential immunogenic structures for the female immune system. Antiseminal antibodies may threaten early fertilization. The aim of our work is to detect and identify seminal proteins that are related to female isoimmunization. In this report, we quantified serum anti-seminal IgG antibodies. Seminal proteins were analysed by two-dimensional gel electrophoresis followed by immunoblotting. To identify IgG-binding proteins of interest, a proteomic approach was selected. The dominant seminal antigens were detected within the rel

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Katakura, Yoshio, Takahiro Miyazaki, Hitomi Wada, et al. "Control of antibody–antigen interaction using anion-induced conformational change in antigen peptide." Protein Engineering, Design and Selection 13, no. 10 (2000): 719–24. http://dx.doi.org/10.1093/protein/13.10.719.

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Elkington, Rebecca, Susan Walker, Tania Crough, et al. "Ex Vivo Profiling of CD8+-T-Cell Responses to Human Cytomegalovirus Reveals Broad and Multispecific Reactivities in Healthy Virus Carriers." Journal of Virology 77, no. 9 (2003): 5226–40. http://dx.doi.org/10.1128/jvi.77.9.5226-5240.2003.

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ABSTRACT Human cytomegalovirus (HCMV) can establish both nonproductive (latent) and productive (lytic) infections. Many of the proteins expressed during these phases of infection could be expected to be targets of the immune response; however, much of our understanding of the CD8+-T-cell response to HCMV is mainly based on the pp65 antigen. Very little is known about T-cell control over other antigens expressed during the different stages of virus infection; this imbalance in our understanding undermines the importance of these antigens in several aspects of HCMV disease pathogenesis. In the p

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Koble, Christian, and Bruno Kyewski. "The thymic medulla: a unique microenvironment for intercellular self-antigen transfer." Journal of Experimental Medicine 206, no. 7 (2009): 1505–13. http://dx.doi.org/10.1084/jem.20082449.

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Central tolerance is shaped by the array of self-antigens expressed and presented by various types of thymic antigen-presenting cells (APCs). Depending on the overall signal quality and/or quantity delivered in these interactions, self-reactive thymocytes either apoptose or commit to the T regulatory cell lineage. The cellular and molecular complexity underlying these events has only recently been appreciated. We analyzed the ex vivo presentation of ubiquitous or tissue-restricted self-antigens by medullary thymic epithelial cells (mTECs) and thymic dendritic cells (DCs), the two major APC typ

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