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Dissertations / Theses on the topic 'Proteine phos'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Anba, Jamila. "Biosynthese et exportation d'une proteine periplasmique, phos, chez escherichia coli k-12." Aix-Marseille 2, 1987. http://www.theses.fr/1987AIX22062.

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La surproduction par escherichia coli de la proteine periplasmique affine pour le phosphate (phos) provoque l'accumulation du precurseur de phos a la fois dans la membrane interne et le cytoplasme. Seul le precurseur membranaire peut etre mature et exporte, alors que le precurseur cytoplasmique subit une coupure lente de sa sequence signal et donne naissance a une forme "pseudo-mature" cytoplasmique. Existence d'un captage entre la synthese et l'exportation. Une application du systeme phos a ete realisee pour la production du facteur de liberation de l'hormone de croissance humaine (hgrf) chez e. Coli. En carence de phosphate, la proteine hybride phos-hgrf est synthetisee et exportee vers le periplasme

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2

Loney, Erica. "PhoR, PhoP and MshC: Three essential proteins of Mycobacterium tuberculosis." University of Toledo / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1396606314.

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3

Gardner, Stewart G. "Studies of PhoU in Escherichia coli: Metal Binding, Dimerization,Protein/Protein Interactions, and a Signaling Complex Model." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5685.

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Phosphate is an essential nutrient for all forms of life. Escherichia coli has a PhoR/PhoB two component regulatory system that controls the expression of various genes whose products allow the cell to thrive in low phosphate environments. The signaling mechanism of the PhoR/PhoB system has been studied and the phosphorylation cascade that controls gene expression is well understood. What is still unknown is how PhoR senses the phosphate level of the environment. The PstS, PstC, PstA, PstB, and PhoU proteins play a role in this signal sensing. This work confirms the hypothesis that the PstSCAB complex senses the environmental phosphate and that phosphate signal is passed through PhoU to PhoR. Further, this work characterizes residues important for interaction on PhoU and PhoR and identifies a structural model for interaction. This model points to a potential mechanism for PhoU mediated signaling to PhoR. We tested this model with direct coupling analysis and obtained further confirmation. Further use of these techniques may elucidate more of the interactions necessary for proper phosphate signaling.

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4

Anba, Jamila. "Biosynthèse et exportation d'une protéine périplasmique, PhoS, chez Escherichia coli K-12." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37602327q.

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5

Johns, Kristine Dawn. "Evidences for Protein-Protein Interactions Between PstB and PhoU in the Phosphate Signaling Complex of Escherichia coli." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/3932.

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The PstSCAB2 complex serves the dual function of being a phosphate transporter as well as the primary sensor of phosphate for the Pho regulon. PhoU is an integral protein required for the signal from PstSCAB2 to be transmitted to PhoR. Our hypothesis is that conformational changes of PstSCAB2 during the phosphate transport process are the mechanism by which information about environmental phosphate levels are transduced to the cell. Additionally, we propose that direct protein-protein interactions between PhoU and the alternating conformations of PstSCAB2 mediate PhoU interactions with PhoR. By means of genetic and biochemical approaches, we have found substantial evidence supporting both these hypotheses.

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6

Cho, Uhn-Soo. "Structural studies of protein phosphatase 2A, simian virus 40 small t antigen, and the PhoQ sensor domain /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/5677.

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7

Ghimire, Jenisha. "Role of Ime4 Protein in PHO Regulon of S.cerevisiae." ScholarWorks@UNO, 2015. http://scholarworks.uno.edu/td/2037.

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In the yeast Saccharomyces cerevisiae, the IME4 methyltransferase, interacts genetically with methyl binding protein, Pho92, to affect the expression of PHO regulon target genes. Cells mutant in IME4 or PHO92 show increases in the RNA abundance of PHO regulon target genes. The increase in the RNA abundance of the PHO regulon target genes is not additive in the cells double mutant in IME4 and PHO92. Hence, Ime4 and Pho92 interact in a single pathway in PHO regulon. Surprisingly, cells overexpressing IME4 and MUM2 shows increase in some PHO regulon target genes, indicating that IME4 affects the PHO regulon target genes through multiple mechanisms in different conditions. A promoter swap experiment revealed that one of the PHO regulon mRNAs that codes for phosphatase, PHO5, is a direct target of Ime4. Further experiments are required to examine whether the same is true for all PHO regulon mRNAs.

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8

Gardner, Stewart G. "Genetic analysis of conserved residues in PhoU of Escherichia coli." Diss., CLICK HERE for online access, 2005. http://contentdm.lib.byu.edu/ETD/image/etd934.pdf.

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9

Pang, Tin Yau. "Study of the surface structures and patterns of the hydrophobic-polar model of protein /." View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202007%20PANG.

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10

Ma, Chun-Wai. "Aboav-Weaire law in complex network and its applications in bioinformatics /." View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?PHYS%202005%20MA.

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11

GUIGUENO, AGNES. "Relation entre la toxicite, le repliement et l'exportation de proteines produits dans le periplasme de escherichia coli : analyse physiologique et genetique a l'aide de proteines hybrides dsba'-phoa." Paris, Institut national d'agronomie de Paris Grignon, 1998. http://www.theses.fr/1998INAP0001.

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La production massive de proteines dans le periplasme de escherichia coli provoque souvent des dysfonctionnements pouvant conduire a la mort cellulaire. Nous les avons analyses a l'aide de proteines hybrides modeles dsba'-phoa, obtenues par insertion du transposon tnphoa dans le gene dsba qui specifie la proteine disulfure oxydase dsba. Les proteines hybrides sont distinctes par la taille du fragment amino-terminal dsba'. Nous montrons que la region gln146-pro163 de dsba est indispensable pour le repliement de dsba et son activite. Les proteines hybrides contenant cette region (classe i) sont stables dans le periplasme contrairement a celles qui en sont depourvues (classe ii). Ces dernieres s'exportent moins bien que les proteines de classe i et inhibent particulierement le processus general d'exportation. A niveau moyen d'expression, elles ont un effet letal dans des bacteries depourvues de la protease periplasmique degp. En degradant le fragment dsba' non structure, degp protege les bacteries. La toxicite des proteines de classe ii dans une souche degp#- peut etre supprimee par des mutations chromosomiques qui reduisent toutes la synthese proteique generale. Certaines d'entre elles ameliorent l'exportation des proteines hybrides. La recherche de genes suppresseurs en copies multiples a permis d'identifier hptr, qui specifie un nouvel arn non traduit de 92 nucleotides. Sa surexpression reduit la quantite de proteine hybride bloquee dans la membrane et ameliore l'exportation d'autres proteines periplasmiques. L'arn hptr pourrait avoir une fonction regulatrice d'un type nouveau.

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12

Nordesjö, Olle. "Searching for novel protein-protein specificities using a combined approach of sequence co-evolution and local structural equilibration." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-275040.

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Greater understanding of how we can use protein simulations and statistical characteristics of biomolecular interfaces as proxies for biological function will make manifest major advances in protein engineering. Here we show how to use calculated change in binding affinity and coevolutionary scores to predict the functional effect of mutations in the interface between a Histidine Kinase and a Response Regulator. These proteins participate in the Two-Component Regulatory system, a system for intracellular signalling found in bacteria. We find that both scores work as proxies for functional mutants and demonstrate a ~30 fold improvement in initial positive predictive value compared with choosing randomly from a sequence space of 160 000 variants in the top 20 mutants. We also demonstrate qualitative differences in the predictions of the two scores, primarily a tendency for the coevolutionary score to miss out on one class of functional mutants with enriched frequency of the amino acid threonine in one position.

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13

Nakamura, Kenichi. "Phophilin, a small GTPase Pho-binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of sperm tail." Kyoto University, 1999. http://hdl.handle.net/2433/181745.

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14

Machaj, Agnieszka S. "Breast Cancer in PTEN Hamartoma Tumor Syndrome: Can a Predictive Fingerprint be Identified?" Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1397736695.

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15

Kwasnicka, Agnieszka. "Identification of PhoP-PhoQ homologues in Pseudomonas aeruginosa responsible for regulation of the outer membrane protein OprH." Thesis, 1999. http://hdl.handle.net/2429/9660.

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Expression of the Pseudomonas aeruginosa outer membrane protein OprH is induced in low magnesium growth conditions (Nicas and Hancock, 1980; 1983). This protein has been proposed to play a role in stabilizing the outer membrane in the absence of Mg²⁺ by interacting with LPS at sites where these cations would bind. Adaptation to magnesium limitation in Salmonella typhimurium has been shown to occur through activation of the two-component regulatory system, PhoP-PhoQ (Soncini et al., 1996). Putative PhoP and PhoQ proteins were identified in the P. aeruginosa genome through homology searches using the corresponding S. typhimurium protein sequences. The genes encoding these proteins were located directly downstream of the gene encoding OprH. Transcriptional linkage of oprH, phoP and phoQ was demonstrated and the hypothesis that this system regulates expression of OprH in P. aeruginosa was tested in the following study. Through construction of aphoP null mutants and transformation of this mutant with PhoP encoding plasmids, it was shown that PhoP is required for expression of OprH. Furthermore, PhoP was demonstrated to be an activator of oprH, phoP and phoQ transcription from a promoter upstream of oprH. In contrast, a phoQ null mutant showed high-level, unregulated activation of oprH and phoP transcription and OprH expression. Complementation of this mutant demonstrated a requirement for PhoQ in down regulation of transcription and response to magnesium. Analysis of the oprH promoter enabled identification of the start of transcription and delineation of the sequences required for regulated OprH expression to within 90 basepairs of the ATG.

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16

Spierings, Aldegonda Maria Johanna. "Enterobacterial species-specific DNA probes based on the gene for outer membrane proteine PhoE = Enterobacteriële species-specifieke DNA probes gebaseerd op het gen coderend voor het buitenmembraaneiwit PhoE /." 1992. http://www.gbv.de/dms/bs/toc/131131567.pdf.

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17

Kelly, Mallory Anne. "Determining the roel of astringency mechanism of whey protein at acidic pHs." 2009. http://www.lib.ncsu.edu/theses/available/etd-08282009-170003/unrestricted/etd.pdf.

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18

Baumgarth, Birgit [Verfasser]. "Molekularbiologische Charakterisierung des ExpG-Proteins aus Sinorhizobium meliloti und Untersuchungen zur komplexen Regulation der exp-Genexpression durch MucR, PhoB und ExpG / vorgelegt von Birgit Baumgarth." 2004. http://d-nb.info/97248292X/34.

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19

Fojtíková, Veronika. "Určení kinetických parametrů pro enzymovou reakci katalyzovanou histidinkinasou s globinovou strukturou senzorové domény." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-333035.

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Two-component signal systems serve as basic stimulus-response coupling mechanism to allow organisms (predominantly bacteria) to sense and respond to changes in many environmental conditions. The prototypical system consists of two proteins, namely a histidine kinase, containing a sensor domain and catalytic kinase core, and a response regulator protein (RR protein). Extracellular stimuli are sensed by a histidine kinase sensor domain. Then ATP is bound to the catalytic kinase core and the γ-phosphoryl group is transferred to the conserved histidine residue. This phosphoryl group is subsequently transferred to a conserved aspartate residue within the RR protein. Phosphotransfer to the RR protein results in activation of a downstream effector domain that elicits the specific response (usually it is transcription activity, but a few RR proteins function as enzymes). The histidine kinase sensor domain is designed for specific ligand interactions. This master thesis focused on the unique histidine kinase containing a sensor domain with a globine structure, which coordinates a heme molecule, namely globin-coupled histidine kinase from Anaeromyxobacter sp. Fw 109-5 (AfGcHK) and its appropriate RR protein. The aim of this thesis was to study and characterize the phosphorylation activity of AfGcHK and RR...

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20

Atwood, Jane Adair. "Recombinant Escherichia coli producing an immobilised functional protein at the surface of bio-polyester beads : a novel application for a bio-bead : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand." 2008. http://hdl.handle.net/10179/818.

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Polyhydroxyalkanoates (PHAs) are polyesters, produced by many bacteria and some archaea. The most commonly characterised is polyhydroxybutyrate (PHB). Produced when nutrients are growth limiting and carbon available in excess, PHA serves as a carbon-energy storage material and forms generally spherical insoluble inclusions between 50-500nm in diameter in the cytoplasm. The key enzyme for PHA synthesis is the PHA synthase and this enzyme catalyses the polymerisation of (R)-3-hydroxy fatty acids into PHA. PHA synthase remains covalently attached to the growing polyester chain and is displayed on the surface of the PHA granule. Other proteins associated with PHA granules include depolymerases for mobilisation or degradation of granules, regulatory proteins and phasins, proteins that aid in PHA granule stability. PHA bio-beads displaying an IgG binding protein were produced and used to purify IgG from serum demonstrating that the PHA synthase can be engineered to display functional synthase fusion proteins at the PHA granule surface. Correctly folded eukaryotic proteins were also produced and displayed at the PHA granule surface as phasin fusion proteins. Multiple-functionality was also achievable by co-expression of various hybrid genes suggesting that this biotechnological bead production strategy might represent a versatile platform technology. The production of functional eukaryotic proteins at the PHA bead surface represents a novel in vivo matrix-assisted protein folding system. Protein engineering of PHA granule surface proteins provides a novel molecular tool for the display of antigens for FACS based analysis and offers promising possibilities in the development of future biotechnological production processes. Overall, the results obtained in this study strongly enhance the applied potential of these polyester beads in biotechnology and medicine.

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