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Journal articles on the topic 'Proteine rvsp'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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1

Kim, Young Soo, Wei Li, Ji Hye Kim, Hwan-Suck Chung, and Jang-Gi Choi. "Anti-Influenza Activity of an Ethyl Acetate Fraction of a Rhus verniciflua Ethanol Extract by Neuraminidase Inhibition." Oxidative Medicine and Cellular Longevity 2020 (October 29, 2020): 1–15. http://dx.doi.org/10.1155/2020/8824934.

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Antigenic mismatch can cause influenza vaccines to be ineffective, and influenza viruses resistant to antiviral drugs are rising. Thus, development of antiviral agents against these viruses is an immediate need. Rhus verniciflua (RVS) has long been used in herbal medicine and as a nutritional supplement. The effect of RVS and its components on influenza virus has not, however, been reported. We found that RVS treatment significantly reduced viral replication when evaluated with green fluorescent protein- (GFP-) tagged virus (influenza A virus, A/PR/8/34-GFP) in Madin–Darby canine kidney (MDCK)

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2

Tran, Pham-Tue-Hung, Naveed Asghar, Urban Höglund, et al. "Development of a Multivalent Kunjin Virus Reporter Virus-Like Particle System Inducing Seroconversion for Ebola and West Nile Virus Proteins in Mice." Microorganisms 8, no. 12 (2020): 1890. http://dx.doi.org/10.3390/microorganisms8121890.

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Kunjin virus (KUNV) is an attenuated strain of the severe neurotropic West Nile virus (WNV). The virus has a single-strand positive-sense RNA genome that encodes a polyprotein. Following gene expression, the polyprotein is cleaved into structural proteins for viral packaging and nonstructural proteins for viral replication and expression. Removal of the structural genes generate subgenomic replicons that maintain replication capacity. Co-expression of these replicons with the viral structural genes produces reporter virus-like particles (RVPs) which infect cells in a single round. In this stud

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3

Bohning, Kelly, Stephanie Sonnberg, Hui-Ling Chen, et al. "A high throughput reporter virus particle microneutralization assay for quantitation of Zika virus neutralizing antibodies in multiple species." PLOS ONE 16, no. 4 (2021): e0250516. http://dx.doi.org/10.1371/journal.pone.0250516.

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Zika virus is a Flavivirus, transmitted via Aedes mosquitos, that causes a range of symptoms including Zika congenital syndrome. Zika has posed a challenging situation for health, public and economic sectors of affected countries. To quantitate Zika virus neutralizing antibody titers in serum samples, we developed a high throughput plate based Zika virus reporter virus particle (RVP) assay that uses an infective, non-replicating particle encoding Zika virus surface proteins and capsid (CprME) and a reporter gene (Renilla luciferase). This is the first characterization of a Zika virus RVP assay

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Sun, Cheuk-Kwan, Yen-Yi Zhen, Hung-I. Lu, et al. "Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model." Stem Cells International 2014 (2014): 1–19. http://dx.doi.org/10.1155/2014/316214.

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We tested the hypothesis that Lipofectamine siRNA delivery to deplete transient receptor potential cation channel (TRPC) 1 protein expression can suppress hypoxia-induced pulmonary arterial hypertension (PAH) in mice. Adult male C57BL/6 mice were equally divided into group 1 (normal controls), group 2 (hypoxia), and group 3 (hypoxia + siRNA TRPC1). By day 28, right ventricular systolic pressure (RVSP), number of muscularized arteries, right ventricle (RV), and lung weights were increased in group 2 than in group 1 and reduced in group 3 compared with group 2. Pulmonary crowded score showed sim

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Morawe, Tobias, Mona Honemann-Capito, Walter von Stein, and Andreas Wodarz. "Loss of the extraproteasomal ubiquitin receptor Rings lost impairs ring canal growth in Drosophila oogenesis." Journal of Cell Biology 193, no. 1 (2011): 71–80. http://dx.doi.org/10.1083/jcb.201009142.

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In Drosophila melanogaster oogenesis, there are 16 germline cells that form a cyst and stay connected to each other by ring canals. Ring canals allow the cytoplasmic transport of proteins, messenger ribonucleic acids, and yolk components from the nurse cells into the oocyte. In this paper, we describe the protein Rings lost (Rngo) and show that it is required for ring canal growth in germline cysts. rngo is an essential gene, and germline clones of a rngo-null allele show defects in ovary development, including mislocalization of ring canal proteins and fusion of germline cells. Rngo appears t

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Ju, Jeong-Sun, Rodrigo A. Fuentealba, Sara E. Miller, et al. "Valosin-containing protein (VCP) is required for autophagy and is disrupted in VCP disease." Journal of Cell Biology 187, no. 6 (2009): 875–88. http://dx.doi.org/10.1083/jcb.200908115.

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Mutations in valosin-containing protein (VCP) cause inclusion body myopathy (IBM), Paget's disease of the bone, and frontotemporal dementia (IBMPFD). Patient muscle has degenerating fibers, rimmed vacuoles (RVs), and sarcoplasmic inclusions containing ubiquitin and TDP-43 (TARDNA-binding protein 43). In this study, we find that IBMPFD muscle also accumulates autophagosome-associated proteins, Map1-LC3 (LC3), and p62/sequestosome, which localize to RVs. To test whether VCP participates in autophagy, we silenced VCP or expressed adenosine triphosphatase–inactive VCP. Under basal conditions, loss

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7

Hanna, Sheri L., Theodore C. Pierson, Melissa D. Sanchez, Asim A. Ahmed, Mariam M. Murtadha, and Robert W. Doms. "N-Linked Glycosylation of West Nile Virus Envelope Proteins Influences Particle Assembly and Infectivity." Journal of Virology 79, no. 21 (2005): 13262–74. http://dx.doi.org/10.1128/jvi.79.21.13262-13274.2005.

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ABSTRACT West Nile virus (WNV) encodes two envelope proteins, premembrane (prM) and envelope (E). While the prM protein of all WNV strains contains a single N-linked glycosylation site, not all strains contain an N-linked site in the E protein. The presence of N-linked glycosylation on flavivirus E proteins has been linked to virus production, pH sensitivity, and neuroinvasiveness. Therefore, we examined the impact of prM and E glycosylation on WNV assembly and infectivity. Similar to other flaviviruses, expression of WNV prM and E resulted in the release of subviral particles (SVPs). Removing

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Yip, Matthew C. J., Nicholas O. Bodnar, and Tom A. Rapoport. "Ddi1 is a ubiquitin-dependent protease." Proceedings of the National Academy of Sciences 117, no. 14 (2020): 7776–81. http://dx.doi.org/10.1073/pnas.1902298117.

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TheSaccharomyces cerevisiaeprotein Ddi1 and its homologs in higher eukaryotes have been proposed to serve as shuttling factors that deliver ubiquitinated substrates to the proteasome. Although Ddi1 contains both ubiquitin-interacting UBA and proteasome-interacting UBL domains, the UBL domain is atypical, as it binds ubiquitin. Furthermore, unlike other shuttling factors, Ddi1 and its homologs contain a conserved helical domain (helical domain of Ddi1, HDD) and a retroviral-like protease (RVP) domain. The RVP domain is probably responsible for cleavage of the precursor of the transcription fact

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9

Reuter, Jon D., Beatriz E. Vivas-Gonzalez, Daniel Gomez, et al. "Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease." Journal of Virology 76, no. 17 (2002): 8900–8909. http://dx.doi.org/10.1128/jvi.76.17.8900-8909.2002.

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ABSTRACT Immunizations with live recombinant vesicular stomatitis viruses (rVSV) expressing foreign viral proteins have successfully protected animals from challenges with several heterologous viruses. We developed an rVSV expressing the major capsid protein (L1) of cottontail rabbit papillomavirus (CRPV) and tested the efficacy of protection following CRPV challenge. An rVSV expressing L1 of CRPV (VSV-L1) was characterized for the protective ability afforded by intranasal, intradermal, or intramuscular vaccination in rabbits subsequently challenged with CRPV. Protein expression of L1 in VSV-L

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10

DeMarco, Vincent G., Javad Habibi, Adam T. Whaley-Connell, et al. "Oxidative stress contributes to pulmonary hypertension in the transgenic (mRen2)27 rat." American Journal of Physiology-Heart and Circulatory Physiology 294, no. 6 (2008): H2659—H2668. http://dx.doi.org/10.1152/ajpheart.00953.2007.

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The transgenic (mRen2)27 (Ren2) rat overexpresses mouse renin in extrarenal tissues, causing increased local synthesis of ANG II, oxidative stress, and hypertension. However, little is known about the role of oxidative stress induced by the tissue renin-angiotensin system (RAS) as a contributing factor in pulmonary hypertension (PH). Using male Ren2 rats, we test the hypothesis that lung tissue RAS overexpression and resultant oxidative stress contribute to PH and pulmonary vascular remodeling. Mean arterial pressure (MAP), right ventricular systolic pressure (RVSP), and wall thickness of smal

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11

van den Pol, Anthony N., Kevin P. Dalton, and John K. Rose. "Relative Neurotropism of a Recombinant Rhabdovirus Expressing a Green Fluorescent Envelope Glycoprotein." Journal of Virology 76, no. 3 (2002): 1309–27. http://dx.doi.org/10.1128/jvi.76.3.1309-1327.2002.

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ABSTRACT A new recombinant vesicular stomatitis virus (rVSV) that expresses green fluorescent protein (GFP) on the cytoplasmic domain of the VSV glycoprotein (G protein) was used in the mouse as a model for studying brain infections by a member of the Mononegavirales order that can cause permanent changes in behavior. After nasal administration, virus moved down the olfactory nerve, first to periglomerular cells, then past the mitral cell layer to granule cells, and finally to the subventricular zone. Eight days postinoculation, rVSV was eliminated from the olfactory bulb. Little sign of infec

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Ou, Zhi-Jun, Wei Wei, Da-De Huang, et al. "l-Arginine restores endothelial nitric oxide synthase-coupled activity and attenuates monocrotaline-induced pulmonary artery hypertension in rats." American Journal of Physiology-Endocrinology and Metabolism 298, no. 6 (2010): E1131—E1139. http://dx.doi.org/10.1152/ajpendo.00107.2010.

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l-Arginine can attenuate pulmonary hypertension (PH) by a mechanism that are not fully understood. This study investigated the molecule mechanism of l-arginine attenuating PH. Sprague Dawley rats were treated with monocrotaline (MCT) with or without l-arginine for 3 or 5 wk. Right ventricular systolic pressure (RVSP), right heart hypertrophy, survival rate, pulmonary artery wall thickness, nitric oxide (NO) concentration, and superoxide anion (O2·−) generation in the lung were measured. Expressions of endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90), phosphorylation o

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13

Liu, Yang, Pengwei Huang, Ming Tan, et al. "Rotavirus VP8*: Phylogeny, Host Range, and Interaction with Histo-Blood Group Antigens." Journal of Virology 86, no. 18 (2012): 9899–910. http://dx.doi.org/10.1128/jvi.00979-12.

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The distal portion of rotavirus (RV) VP4 spike protein (VP8*) is implicated in binding to cellular receptors, thereby facilitating viral attachment and entry. While VP8* of some animal RVs engage sialic acid, human RVs often attach to and enter cells in a sialic acid-independent manner. A recent study demonstrated that the major human RVs (P[4], P[6], and P[8]) recognize human histo-blood group antigens (HBGAs). In this study, we performed a phylogenetic analysis of RVs and showed further variations of RV interaction with HBGAs. On the basis of the VP8* sequences, RVs are grouped into five P g

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Cruz, J. Agustin, Eileen M. Bauer, Andres I. Rodriguez, et al. "Chronic hypoxia induces right heart failure in caveolin-1−/− mice." American Journal of Physiology-Heart and Circulatory Physiology 302, no. 12 (2012): H2518—H2527. http://dx.doi.org/10.1152/ajpheart.01140.2011.

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Caveolin-1 (Cav-1)−/− mice develop mild pulmonary hypertension as they age. In this study, we sought to determine the effect of chronic hypoxia, an established model of pulmonary hypertension, on young Cav-1−/− mice with no measurable signs of pulmonary hypertension. Exposure of Cav-1−/− mice to chronic hypoxia resulted in an initial rise in right ventricular (RV) systolic pressure (RVSP) similar to wild-type (WT) mice. By three weeks RVSP decreased in the Cav-1−/− mice, whereas it was maintained in WT mice. The drop in RVSP in Cav-1−/− mice was accompanied by decreased cardiac output, increas

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15

Youn, Ji-Young, Helena Friesen, Takuma Kishimoto, et al. "Dissecting BAR Domain Function in the Yeast Amphiphysins Rvs161 and Rvs167 during Endocytosis." Molecular Biology of the Cell 21, no. 17 (2010): 3054–69. http://dx.doi.org/10.1091/mbc.e10-03-0181.

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BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in the budding yeast Saccharomyces cerevisiae, Rvs161 and Rvs167, are required for endocytosis. We have explored the mechanism of N-BAR-domain function in the endocytosis process using a combined biochemical and genetic approach. We show that the purified Rvs161–Rvs167 complex binds to liposomes in a curvature-indepen

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16

Chaisakul, Janeyuth, Orawan Khow, Kulachet Wiwatwarayos, et al. "A Biochemical and Pharmacological Characterization of Phospholipase A2 and Metalloproteinase Fractions from Eastern Russell’s Viper (Daboia siamensis) Venom: Two Major Components Associated with Acute Kidney Injury." Toxins 13, no. 8 (2021): 521. http://dx.doi.org/10.3390/toxins13080521.

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Acute kidney injury (AKI) following Eastern Russell’s viper (Daboia siamensis) envenoming is a significant symptom in systemically envenomed victims. A number of venom components have been identified as causing the nephrotoxicity which leads to AKI. However, the precise mechanism of nephrotoxicity caused by these toxins is still unclear. In the present study, we purified two proteins from D. siamensis venom, namely RvPLA2 and RvMP. Protein identification using LCMS/MS confirmed the identity of RvPLA2 to be snake venom phospholipase A2 (SVPLA2) from Thai D. siamensis venom, whereas RvMP exhibit

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17

West, James, Julie Harral, Kirk Lane, et al. "Mice expressing BMPR2R899X transgene in smooth muscle develop pulmonary vascular lesions." American Journal of Physiology-Lung Cellular and Molecular Physiology 295, no. 5 (2008): L744—L755. http://dx.doi.org/10.1152/ajplung.90255.2008.

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Familial pulmonary arterial hypertension (PAH) is associated with mutations in bone morphogenetic protein type II receptor (BMPR2). Many of these mutations occur in the BMPR2 tail domain, leaving the SMAD functions intact. To determine the in vivo consequences of BMPR2 tail domain mutation, we created a smooth muscle-specific doxycycline-inducible BMPR2 mutation with an arginine to termination mutation at amino acid 899. When these SM22-rtTA x TetO7-BMPR2R899X mice had transgene induced for 9 wk, starting at 4 wk of age, they universally developed pulmonary vascular pruning as assessed by fluo

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Mire, Chad E., Derek Dube, Sue E. Delos, Judith M. White, and Michael A. Whitt. "Glycoprotein-Dependent Acidification of Vesicular Stomatitis Virus Enhances Release of Matrix Protein." Journal of Virology 83, no. 23 (2009): 12139–50. http://dx.doi.org/10.1128/jvi.00955-09.

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ABSTRACT To study vesicular stomatitis virus (VSV) entry and uncoating, we generated a recombinant VSV encoding a matrix (M) protein containing a C-terminal tetracysteine Lumio tag (rVSV-ML) that could be fluorescently labeled using biarsenical compounds. Quantitative confocal microscopy showed that there is a transient loss of fluorescence at early times after the initiation of endocytosis of rVSV-ML-Green (rVSV-MLG) virions, which did not occur when cells were treated with bafilomycin A1. The reduction in fluorescence occurred 5 to 10 min postentry, followed by a steady increase in fluoresce

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Yu, Lei, Yingfeng Tu, Xueling Jia, et al. "Resveratrol Protects Against Pulmonary Arterial Hypertension in Rats via Activation of Silent Information Regulator 1." Cellular Physiology and Biochemistry 42, no. 1 (2017): 55–67. http://dx.doi.org/10.1159/000477115.

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Background/Objectives: The polyphenol resveratrol (Rev) has been found to exhibit various beneficial effects including prevention of pulmonary arterial hypertension (PAH). The present study was designed to investigate the action and potential mechanism of Rev on PAH, focusing on the role of SIRT1 (Silent Information Regulator 1) in apoptosis of pulmonary artery smooth muscle cells (PASMCs). Methods: PAH rats were established by exposure to hypoxia for 21 days. Rev and SRT1720 (a selective SIRT1 activator) were used to reverse PAH by gavaging rats. PASMCs were confronted with hypoxia for 24 h o

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Diggins, Nicole L., and Donna J. Webb. "APPL1 is a multifunctional endosomal signaling adaptor protein." Biochemical Society Transactions 45, no. 3 (2017): 771–79. http://dx.doi.org/10.1042/bst20160191.

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Endosomal adaptor proteins are important regulators of signaling pathways underlying many biological processes. These adaptors can integrate signals from multiple pathways via localization to specific endosomal compartments, as well as through multiple protein–protein interactions. One such adaptor protein that has been implicated in regulating signaling pathways is the adaptor protein containing a pleckstrin homology (PH) domain, phosphotyrosine-binding (PTB) domain, and leucine zipper motif 1 (APPL1). APPL1 localizes to a subset of Rab5-positive endosomes through its Bin–Amphiphysin–Rvs and

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CELLA, GIUSEPPE, FABRIZIO VIANELLO, FRANCO COZZI, et al. "Effect of Bosentan on Plasma Markers of Endothelial Cell Activity in Patients with Secondary Pulmonary Hypertension Related to Connective Tissue Diseases." Journal of Rheumatology 36, no. 4 (2009): 760–67. http://dx.doi.org/10.3899/jrheum.080542.

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Objective.To evaluate plasma markers of endothelial cell activity in patients with pulmonary arterial hypertension (PAH) induced by connective tissue diseases (CTD) before and after 3-month administration of bosentan.Methods.We quantified E, L and P-selectin (sE-S, sL-S, sP-S), thrombomodulin (TM), monocyte-chemotactic protein 1 (MCP-1), human soluble CD40 ligand (sCD40L), and nitric oxide (NO) in 18 patients and 18 controls. We evaluated right ventricular systolic pressure (RVSP) and the 6-minute walk test (6-MWT).Results.All plasma markers but sL-S and TM at Time 0 were significantly higher

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Cero, Fadila Telarevic, Vigdis Hillestad, Ivar Sjaastad, et al. "Absence of the inflammasome adaptor ASC reduces hypoxia-induced pulmonary hypertension in mice." American Journal of Physiology-Lung Cellular and Molecular Physiology 309, no. 4 (2015): L378—L387. http://dx.doi.org/10.1152/ajplung.00342.2014.

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Pulmonary hypertension is a serious condition that can lead to premature death. The mechanisms involved are incompletely understood although a role for the immune system has been suggested. Inflammasomes are part of the innate immune system and consist of the effector caspase-1 and a receptor, where nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) is the best characterized and interacts with the adaptor protein apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC). To investigate whether ASC and NLRP3 inflammasome compone

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HEMSLEY, S., N. COLE, P. CANFIELD, and M. D. P. WILLCOX. "Protein microanalysis of animal tears." Research in Veterinary Science 68, no. 3 (2000): 207–9. http://dx.doi.org/10.1053/rvsc.1999.0358.

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Douglas, Lois M., Stephen W. Martin, and James B. Konopka. "BAR Domain Proteins Rvs161 and Rvs167 Contribute to Candida albicans Endocytosis, Morphogenesis, and Virulence." Infection and Immunity 77, no. 9 (2009): 4150–60. http://dx.doi.org/10.1128/iai.00683-09.

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ABSTRACT The Candida albicans plasma membrane plays critical roles in growth and virulence and as a target for antifungal drugs. Three C. albicans genes that encode Bin-Amphiphysin-Rvs homology domain proteins were mutated to define their roles in plasma membrane function. The deletion of RVS161 and RVS167, but not RVS162, caused strong defects. The rvs161Δ mutant was more defective in endocytosis and morphogenesis than rvs167Δ, but both were strongly defective in polarizing actin patches. Other plasma membrane constituents were still properly localized, including a filipin-stained domain at t

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Pranesh and S. Ramesh. "Variability for grain protein content among germplasm accessions and advanced breeding lines in dolichos bean (Lablab purpureus L. Sweet var. Lignosus Prain)." Plant Genetic Resources: Characterization and Utilization 17, no. 03 (2018): 289–92. http://dx.doi.org/10.1017/s1479262118000424.

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AbstractProtein energy malnutrition (PEM) is prevalent in south-east Asian countries including India. Breeding and introduction of grain protein-rich varieties of legumes such as dolichos bean is considered as cost-effective approach to combat PEM. Exploitation of genetic variability within germplasm accessions (GAs) and/or breeding populations is the short-term strategy for identification and delivery of protein-rich dolichos bean cultivars to cater to the immediate needs of the farmers and target population. A set of 118 dolichos bean genotypes consisting of 96 GAs and 20 advanced breeding l

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Merrill, Liana, Susan Malley, and Margaret A. Vizzard. "Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 305, no. 2 (2013): R147—R156. http://dx.doi.org/10.1152/ajpregu.00089.2013.

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Stress exacerbates symptoms of functional lower urinary tract disorders including interstitial cystitis (IC)/bladder pain syndrome (BPS) and overactive bladder (OAB) in humans, but mechanisms contributing to symptom worsening are unknown. These studies address stress-induced changes in the structure and function of the micturition reflex using an animal model of stress in male rats. Rats were exposed to 7 days of repeated variate stress (RVS). Target organ (urinary bladder, thymus, adrenal gland) tissues were collected and weighed following RVS. Evans blue (EB) concentration and histamine, mye

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Ke, Xingrao, Hollis Johnson, Xigang Jing, et al. "Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model." Physiological Genomics 50, no. 10 (2018): 828–36. http://dx.doi.org/10.1152/physiolgenomics.00047.2018.

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Decreased expression of endothelial nitric oxide synthase (eNOS), a key mediator of perinatal transition, characterizes persistent pulmonary hypertension of the newborn (PPHN) in neonates and a fetal lamb model; the mechanisms are unclear. We investigated whether increased DNA CpG methylation at the eNOS promoter in estrogen response elements (EREs) and altered histone code together contribute to decreased eNOS expression in PPHN. We isolated pulmonary artery endothelial cells (PAEC) from fetal lambs with PPHN induced by prenatal ductus arteriosus constriction from 128 to 136 days gestation or

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Gkourtsa, Areti, Janny van den Burg, Karin Strijbis, et al. "Identification and Characterization of Rvs162/Rvs167-3, a Novel N-BAR Heterodimer in the Human Fungal Pathogen Candida albicans." Eukaryotic Cell 14, no. 2 (2014): 182–93. http://dx.doi.org/10.1128/ec.00282-14.

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ABSTRACT Membrane reshaping resides at the core of many important cellular processes, and among its mediators are the BAR (Bin, Amphiphysin, Rvs) domain-containing proteins. We have explored the diversity and function of the Rvs BAR proteins in Candida albicans and identified a novel family member, Rvs167-3 (orf19.1861). We show that Rvs167-3 specifically interacts with Rvs162 to form a stable BAR heterodimer able to bind liposomes in vitro . A second, distinct heterodimer is formed by the canonical BAR proteins Rvs161 and Rvs167. Purified Rvs161/Rvs167 complex also binds liposomes, indicating

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Yasuda, Tadashi, Yuji Tada, Nobuhiro Tanabe, Koichiro Tatsumi, and James West. "Rho-kinase inhibition alleviates pulmonary hypertension in transgenic mice expressing a dominant-negative type II bone morphogenetic protein receptor gene." American Journal of Physiology-Lung Cellular and Molecular Physiology 301, no. 5 (2011): L667—L674. http://dx.doi.org/10.1152/ajplung.00423.2010.

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Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by a sustained elevation in the pulmonary artery pressure and subsequent right heart failure. The activation of Rho/Rho-kinase activity and the beneficial effect of Rho-kinase inhibition have been demonstrated in several experimental models of pulmonary hypertension. However, it remains unclear whether Rho-kinase inhibitors can also be used against pulmonary hypertension associated with mutations in the type II bone morphogenetic protein receptor (BMPRII) gene. Transgenic mice expressing a dominant-negative BMPRI

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Cooper, David, Kevin J. Wright, Priscilla C. Calderon, et al. "Attenuation of Recombinant Vesicular Stomatitis Virus-Human Immunodeficiency Virus Type 1 Vaccine Vectors by Gene Translocations and G Gene Truncation Reduces Neurovirulence and Enhances Immunogenicity in Mice." Journal of Virology 82, no. 1 (2007): 207–19. http://dx.doi.org/10.1128/jvi.01515-07.

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ABSTRACT Recombinant vesicular stomatitis virus (rVSV) has shown great potential as a new viral vector for vaccination. However, the prototypic rVSV vector described previously was found to be insufficiently attenuated for clinical evaluation when assessed for neurovirulence in nonhuman primates. Here, we describe the attenuation, neurovirulence, and immunogenicity of rVSV vectors expressing human immunodeficiency virus type 1 Gag. These rVSV vectors were attenuated by combinations of the following manipulations: N gene translocations (N4), G gene truncations (CT1 or CT9), noncytopathic M gene

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Karotki, Lena, Juha T. Huiskonen, Christopher J. Stefan, et al. "Eisosome proteins assemble into a membrane scaffold." Journal of Cell Biology 195, no. 5 (2011): 889–902. http://dx.doi.org/10.1083/jcb.201104040.

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Spatial organization of membranes into domains of distinct protein and lipid composition is a fundamental feature of biological systems. The plasma membrane is organized in such domains to efficiently orchestrate the many reactions occurring there simultaneously. Despite the almost universal presence of membrane domains, mechanisms of their formation are often unclear. Yeast cells feature prominent plasma membrane domain organization, which is at least partially mediated by eisosomes. Eisosomes are large protein complexes that are primarily composed of many subunits of two Bin–Amphiphysin–Rvs

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Simunovic, Mijo, Emma Evergren, Ivan Golushko, et al. "How curvature-generating proteins build scaffolds on membrane nanotubes." Proceedings of the National Academy of Sciences 113, no. 40 (2016): 11226–31. http://dx.doi.org/10.1073/pnas.1606943113.

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Bin/Amphiphysin/Rvs (BAR) domain proteins control the curvature of lipid membranes in endocytosis, trafficking, cell motility, the formation of complex subcellular structures, and many other cellular phenomena. They form 3D assemblies that act as molecular scaffolds to reshape the membrane and alter its mechanical properties. It is unknown, however, how a protein scaffold forms and how BAR domains interact in these assemblies at protein densities relevant for a cell. In this work, we use various experimental, theoretical, and simulation approaches to explore how BAR proteins organize to form a

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Gao, H., Y. Dong, RF Machado, and J. Chen. "ID: 9: AN IL7 MONOCLONAL ANTIBODY RESCUES HYPOXIA-MEDIATED PULMONARY HYPERTENSION IN MICE." Journal of Investigative Medicine 64, no. 4 (2016): 921.3–922. http://dx.doi.org/10.1136/jim-2016-000120.22.

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RationalePreviously we showed that human plasma IL7, protein levels of IL7 receptor (IL7R) in total lung homogenate and pulmonary artery endothelial cells (PAECs)/PASMCs were significantly elevated in PAH patients. IL7 promotes PASMC proliferation. IL7R-deficient (IL7R−/−) mice are protected from hypoxia-mediated pulmonary hypertension (HPH). We sought to study whether blocking IL7 via anti-IL7 antibodies can reverse experimental PH in mice.MethodsMale C57BL/6 mice (7 week old) were exposed to normoxia or hypoxia (10% O2) for 4 weeks (n=6 per group). At Day 12 after hypoxia exposure, a monoclo

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Foley, Heather D., Miguel Otero, Jan M. Orenstein, Roger J. Pomerantz, and Matthias J. Schnell. "Rhabdovirus-Based Vectors with Human Immunodeficiency Virus Type 1 (HIV-1) Envelopes Display HIV-1-Like Tropism and Target Human Dendritic Cells." Journal of Virology 76, no. 1 (2002): 19–31. http://dx.doi.org/10.1128/jvi.76.1.19-31.2002.

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ABSTRACT We describe replication-competent, vaccine strain-based rabies viruses (RVs) that lack their own single glycoprotein and express, instead, a chimeric RV-human immunodeficiency virus type 1 (HIV-1) envelope protein composed of the ectodomain and transmembrane domains of HIV-1 gp160 and the cytoplasmic domain of RV G. The envelope proteins from both X4 (NL4-3)- and R5X4 (89.6)-tropic HIV-1 strains were utilized. These recombinant viruses very closely mimicked an HIV-1- like tropism, as indicated by blocking experiments. Infection was inhibited by SDF-1 on cells expressing CD4 and CXCR4

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Castillo-Galán, Sebastian, German A. Arenas, Roberto V. Reyes, Bernardo J. Krause, and Rodrigo Iturriaga. "Stim-activated TRPC-ORAI channels in pulmonary hypertension induced by chronic intermittent hypoxia." Pulmonary Circulation 10, no. 1_suppl (2020): 13–22. http://dx.doi.org/10.1177/2045894020941484.

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Obstructive sleep apnea (OSA), a breathing disorder featured by chronic intermittent hypoxia (CIH) is associated with pulmonary hypertension (PH). Rodents exposed to CIH develop pulmonary vascular remodeling and PH, but the pathogenic mechanisms are not well known. Overexpression of Stim-activated Transient Receptor Potential Channels (TRPC) and Calcium Release-Activated Calcium Channel Protein (ORAI) TRPC-ORAI Ca2+ channels (STOC) has been involved in pulmonary vascular remodeling and PH in sustained hypoxia. However, it is not known if CIH may change STOC levels. Accordingly, we studied the

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Kanai, Yuta, Misa Onishi, Takahiro Kawagishi, et al. "Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus." Journal of Virology 95, no. 2 (2020): e01374-20. http://dx.doi.org/10.1128/jvi.01374-20.

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ABSTRACTSpecies A rotaviruses (RVs) are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years. Currently available RV vaccines were adapted from wild-type RV strains by serial passage of cultured cells or by reassortment between human and animal RV strains. These traditional methods require large-scale screening and genotyping to obtain vaccine candidates. Reverse genetics is a tractable, rapid, and reproducible approach to generating recombinant RV vaccine candidates carrying any VP4 and VP7 genes that provide selected antigenicity. Here, we developed a

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O’DOHERTY, E., M. AHERNE, S. ENNIS, E. WEAVERS, J. F. ROCHE, and T. SWEENEY. "Prion protein gene polymorphisms in pedigree sheep in Ireland." Research in Veterinary Science 70, no. 1 (2001): 51–56. http://dx.doi.org/10.1053/rvsc.2000.0441.

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LUQUE, I., C. TARRADAS, R. ASTORGA, A. PEREA, H. J. WISSELINK, and U. VECHT. "The presence of muramidase released protein and extracellular factor protein in various serotypes ofStreptococcus suisisolated from diseased and healthy pigs in Spain." Research in Veterinary Science 66, no. 1 (1999): 69–72. http://dx.doi.org/10.1053/rvsc.1998.0242.

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Veltman, Douwe M., Giulio Auciello, Heather J. Spence, Laura M. Machesky, Joshua Z. Rappoport, and Robert H. Insall. "Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis." Biochemical Journal 436, no. 1 (2011): 45–52. http://dx.doi.org/10.1042/bj20101684.

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I-BAR (inverse-Bin/amphiphysin/Rvs)-domain-containing proteins such as IRSp53 (insulin receptor substrate of 53 kDa) associate with outwardly curved membranes and connect them to proteins involved in actin dynamics. Research on I-BAR proteins has focussed on possible roles in filopod and lamellipod formation, but their full physiological function remains unclear. The social amoeba Dictyostelium encodes a single I-BAR/SH3 (where SH3 is Src homology 3) protein, called IBARa, along with homologues of proteins that interact with IRSp53 family proteins in mammalian cells, providing an excellent mod

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IWASE, M., K. KIMURA, N. SASAKI, et al. "Canine leptin: cDNA cloning, expression and activity of recombinant protein." Research in Veterinary Science 68, no. 2 (2000): 109–14. http://dx.doi.org/10.1053/rvsc.1999.0342.

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Pinski, Amanda N., and Ilhem Messaoudi. "To B or Not to B: Mechanisms of Protection Conferred by rVSV-EBOV-GP and the Roles of Innate and Adaptive Immunity." Microorganisms 8, no. 10 (2020): 1473. http://dx.doi.org/10.3390/microorganisms8101473.

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Zaire Ebola virus (EBOV) is a member of the Filoviridae family of negative sense, single-stranded RNA viruses. EBOV infection causes Ebola virus disease (EVD), characterized by coagulopathy, lymphopenia, and multi-organ failure, which can culminate in death. In 2019, the FDA approved the first vaccine against EBOV, a recombinant live-attenuated viral vector wherein the G protein of vesicular stomatitis virus is replaced with the glycoprotein (GP) of EBOV (rVSV-EBOV-GP, Ervebo® by Merck). This vaccine demonstrates high efficacy in nonhuman primates by providing prophylactic, rapid, and post-exp

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Rao, Yijian, and Volker Haucke. "Membrane shaping by the Bin/amphiphysin/Rvs (BAR) domain protein superfamily." Cellular and Molecular Life Sciences 68, no. 24 (2011): 3983–93. http://dx.doi.org/10.1007/s00018-011-0768-5.

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Zhong, Ying, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing, and Wenbin Zhan. "Development and Characterization of Monoclonal Antibodies to the 32 kDa Viral Attachment Protein of Lymphocystis Disease Virus and Their Neutralizing Ability in Vitro." International Journal of Molecular Sciences 19, no. 9 (2018): 2536. http://dx.doi.org/10.3390/ijms19092536.

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In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral attachment protein (VAP) that specifically interacted with the 27.8 kDa cellular receptor from flounder Paralichthys olivaceus gill (FG) cells, and the recombinant VAP (rVAP) was expressed in Escherichia coli strain BL21 (DE3). In this study, monoclonal antibodies (MAbs) against 32 kDa VAP are produced by immunization of BALB/c mice with the rVAP. Seven hybridoma secreting MAbs were screened by enzyme-linked immunosorbent assay, five of which designated as 1C6, 1C8, 3B5, 3D11 and 3H10 are cloned

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Wang, Liang, Ziyi Yan, Helena Vihinen, et al. "FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function." Journal of Cell Biology 218, no. 1 (2018): 97–111. http://dx.doi.org/10.1083/jcb.201806191.

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Mitochondrial function is closely linked to its dynamic membrane ultrastructure. The mitochondrial inner membrane (MIM) can form extensive membrane invaginations known as cristae, which contain the respiratory chain and ATP synthase for oxidative phosphorylation. The molecular mechanisms regulating mitochondrial ultrastructure remain poorly understood. The Bin-Amphiphysin-Rvs (BAR) domain proteins are central regulators of diverse cellular processes related to membrane remodeling and dynamics. Whether BAR domain proteins are involved in sculpting membranes in specific submitochondrial compartm

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Vannier, Christian, Arlette Pesty, Mabel Jouve San-Roman, and Anne A. Schmidt. "The Bin/Amphiphysin/Rvs (BAR) Domain Protein Endophilin B2 Interacts with Plectin and Controls Perinuclear Cytoskeletal Architecture." Journal of Biological Chemistry 288, no. 38 (2013): 27619–37. http://dx.doi.org/10.1074/jbc.m113.485482.

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Proteins of the Bin/amphiphysin/Rvs (BAR) domain superfamily are essential in controlling the shape and dynamics of intracellular membranes. Here, we present evidence for the unconventional function of a member of the endophilin family of BAR and Src homology 3 domain-containing proteins, namely endophilin B2, in the perinuclear organization of intermediate filaments. Using mass spectrometry analysis based on capturing endophilin B2 partners in in situ pre-established complexes in cells, we unravel the interaction of endophilin B2 with plectin 1, a variant of the cytoskeleton linker protein pl

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Nishimura, Tamako, Nobuhiro Morone, and Shiro Suetsugu. "Membrane re-modelling by BAR domain superfamily proteins via molecular and non-molecular factors." Biochemical Society Transactions 46, no. 2 (2018): 379–89. http://dx.doi.org/10.1042/bst20170322.

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Lipid membranes are structural components of cell surfaces and intracellular organelles. Alterations in lipid membrane shape are accompanied by numerous cellular functions, including endocytosis, intracellular transport, and cell migration. Proteins containing Bin–Amphiphysin–Rvs (BAR) domains (BAR proteins) are unique, because their structures correspond to the membrane curvature, that is, the shape of the lipid membrane. BAR proteins present at high concentration determine the shape of the membrane, because BAR domain oligomers function as scaffolds that mould the membrane. BAR proteins co-o

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Zhu, Yang, Yuanmei Ma, Mijia Lu, et al. "Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein." Viruses 11, no. 5 (2019): 444. http://dx.doi.org/10.3390/v11050444.

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Human norovirus (HuNoV) is responsible for more than 95% of outbreaks of acute nonbacterial gastroenteritis worldwide. Despite major efforts, there are no vaccines or effective therapeutic interventions against this virus. Chicken immunoglobulin Y (IgY)-based passive immunization has been shown to be an effective strategy to prevent and treat many enteric viral diseases. Here, we developed a highly efficient bioreactor to generate high titers of HuNoV-specific IgY in chicken yolks using a recombinant vesicular stomatitis virus expressing HuNoV capsid protein (rVSV-VP1) as an antigen. We first

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TANIGUCHI, TAKASHI, YOSHIHIDE ASANO, KANAME AKAMATA, et al. "Serum Levels of Galectin-3: Possible Association with Fibrosis, Aberrant Angiogenesis, and Immune Activation in Patients with Systemic Sclerosis." Journal of Rheumatology 39, no. 3 (2012): 539–44. http://dx.doi.org/10.3899/jrheum.110755.

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Objective.Galectin-3 is a multifunctional protein implicated in a variety of biological processes including fibrosis, angiogenesis, and immune activation, all of which are associated with the development of systemic sclerosis (SSc). We investigated the clinical significance of serum galectin-3 levels in SSc.Methods.Serum galectin-3 levels were determined by a specific ELISA in 58 patients with SSc and 19 healthy controls.Results.Serum galectin-3 levels were significantly lower in patients with diffuse cutaneous SSc (dcSSc) than in controls (3.29 ± 3.27 ng/ml vs 4.91 ± 2.67 ng/ml, respectively;

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Tsujita, Kazuya, Shiro Suetsugu, Nobunari Sasaki, Masahiro Furutani, Tsukasa Oikawa, and Tadaomi Takenawa. "Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis." Journal of Cell Biology 172, no. 2 (2006): 269–79. http://dx.doi.org/10.1083/jcb.200508091.

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The conserved FER-CIP4 homology (FCH) domain is found in the pombe Cdc15 homology (PCH) protein family members, including formin-binding protein 17 (FBP17). However, the amino acid sequence homology extends beyond the FCH domain. We have termed this region the extended FC (EFC) domain. We found that FBP17 coordinated membrane deformation with actin cytoskeleton reorganization during endocytosis. The EFC domains of FBP17, CIP4, and other PCH protein family members show weak homology to the Bin-amphiphysin-Rvs (BAR) domain. The EFC domains bound strongly to phosphatidylserine and phosphatidylino

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Doherty, Gary J., and Richard Lundmark. "GRAF1-dependent endocytosis." Biochemical Society Transactions 37, no. 5 (2009): 1061–65. http://dx.doi.org/10.1042/bst0371061.

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The role of endocytosis in controlling a multitude of cell biological events is well established. Molecular and mechanistic characterization of endocytosis has predominantly focused on CME (clathrin-mediated endocytosis), although many other endocytic pathways have been described. It was recently shown that the BAR (Bin/amphiphysin/Rvs) and Rho GAP (GTPase-activating protein) domain-containing protein GRAF1 (GTPase regulator associated with focal adhesion kinase-1) is found on prevalent, pleiomorphic endocytic membranes, and is essential for the major, clathrin-independent endocytic pathway th

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